Structured Review

Agilent technologies hplc system
SDS-PAGE gels stained with Coomassie blue, before and after depletion of the high abundant proteins. Lanes 1, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 are crude plasma samples from subjects enrolled for the analysis. Lanes 2, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25 are <t>MARS</t> “Top-7” <t>HPLC-treated</t> plasma samples for depletion of the high abundance proteins. Lane 3 is empty; M: molecular weight standard. 20 μg of proteins were loaded in each lane.
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1) Product Images from "Plasma Proteomic Profiling in Hereditary Breast Cancer Reveals a BRCA1-Specific Signature: Diagnostic and Functional Implications"

Article Title: Plasma Proteomic Profiling in Hereditary Breast Cancer Reveals a BRCA1-Specific Signature: Diagnostic and Functional Implications

Journal: PLoS ONE

doi: 10.1371/journal.pone.0129762

SDS-PAGE gels stained with Coomassie blue, before and after depletion of the high abundant proteins. Lanes 1, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 are crude plasma samples from subjects enrolled for the analysis. Lanes 2, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25 are MARS “Top-7” HPLC-treated plasma samples for depletion of the high abundance proteins. Lane 3 is empty; M: molecular weight standard. 20 μg of proteins were loaded in each lane.
Figure Legend Snippet: SDS-PAGE gels stained with Coomassie blue, before and after depletion of the high abundant proteins. Lanes 1, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 are crude plasma samples from subjects enrolled for the analysis. Lanes 2, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25 are MARS “Top-7” HPLC-treated plasma samples for depletion of the high abundance proteins. Lane 3 is empty; M: molecular weight standard. 20 μg of proteins were loaded in each lane.

Techniques Used: SDS Page, Staining, High Performance Liquid Chromatography, Molecular Weight

2) Product Images from "Salvia miltiorrhiza (Dan Shen) Significantly Ameliorates Colon Inflammation in Dextran Sulfate Sodium Induced Colitis"

Article Title: Salvia miltiorrhiza (Dan Shen) Significantly Ameliorates Colon Inflammation in Dextran Sulfate Sodium Induced Colitis

Journal: The American journal of Chinese medicine

doi: 10.1142/S0192415X13500742

HPLC analysis of Salvia miltiorrhiza extract (SME) and chemical structures of identified representative constituents in SME. (A) HPLC chromatogram of SME recorded at 280 nm. (B) Chemical structures of four identified compounds.
Figure Legend Snippet: HPLC analysis of Salvia miltiorrhiza extract (SME) and chemical structures of identified representative constituents in SME. (A) HPLC chromatogram of SME recorded at 280 nm. (B) Chemical structures of four identified compounds.

Techniques Used: High Performance Liquid Chromatography

3) Product Images from "Optimization for the Production of Surfactin with a New Synergistic Antifungal Activity"

Article Title: Optimization for the Production of Surfactin with a New Synergistic Antifungal Activity

Journal: PLoS ONE

doi: 10.1371/journal.pone.0034430

HPLC profile of surfactin produced by B. amyloliquefaciens MB199. The elution was monitored at 210 nm at a flow rate of 1 mL/min. The dashed line and the real line represent the HPLC profiles of surfactins produced in optimized and original culture media, respectively.
Figure Legend Snippet: HPLC profile of surfactin produced by B. amyloliquefaciens MB199. The elution was monitored at 210 nm at a flow rate of 1 mL/min. The dashed line and the real line represent the HPLC profiles of surfactins produced in optimized and original culture media, respectively.

Techniques Used: High Performance Liquid Chromatography, Produced, Flow Cytometry

4) Product Images from "A Novel Antioxidant Phenyl Disaccharide from Populus tremula Knotwood"

Article Title: A Novel Antioxidant Phenyl Disaccharide from Populus tremula Knotwood

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

doi: 10.3390/12020205

Analytical HPLC chromatogram of Populus tremula knotwood extract recorded at 314 nm. Inserts represent the chromatograms of the fractions F1 and F2 isolated using preparative HPLC.
Figure Legend Snippet: Analytical HPLC chromatogram of Populus tremula knotwood extract recorded at 314 nm. Inserts represent the chromatograms of the fractions F1 and F2 isolated using preparative HPLC.

Techniques Used: High Performance Liquid Chromatography, Isolation

5) Product Images from "The Scientific Basis and Advantage of Human Experiential Assessment in the quality control of Chinese Herbal Medicines exampling as Schisandrae Chinensis Fructus"

Article Title: The Scientific Basis and Advantage of Human Experiential Assessment in the quality control of Chinese Herbal Medicines exampling as Schisandrae Chinensis Fructus

Journal: Scientific Reports

doi: 10.1038/s41598-018-23619-5

HPLC chromatogram of SCF, the above one was sample solution, and the below one was mixed references solution ( A ). Content of lignans in different part of SCF ( B ). (This image was created by Dr. Zhou).
Figure Legend Snippet: HPLC chromatogram of SCF, the above one was sample solution, and the below one was mixed references solution ( A ). Content of lignans in different part of SCF ( B ). (This image was created by Dr. Zhou).

Techniques Used: High Performance Liquid Chromatography

6) Product Images from "DNA methylation on N6-adenine in mammalian embryonic stem cells"

Article Title: DNA methylation on N6-adenine in mammalian embryonic stem cells

Journal: Nature

doi: 10.1038/nature17640

LC-MS/MS data of N6-mA a N5]N6-mA was used as the internal standard. b , N6-mA levels are ultralow in adult tissues. c , No detection of DNA alkylation adducts, such as N1-mA, N3-mA or N3-mC in mouse ES cells or Alkbh1 knockout cells by MS. d , LC-MS/MS analysis of N1-mA or N6-mA digested from synthetic oligonucleotides (top) and ES cell DNA samples (bottom). e , ESI-QTOF-MS/MS spectra of analytical standard of N6-mA nucleosides (top) and N6-mA containing HPLC fraction from ES cells.
Figure Legend Snippet: LC-MS/MS data of N6-mA a N5]N6-mA was used as the internal standard. b , N6-mA levels are ultralow in adult tissues. c , No detection of DNA alkylation adducts, such as N1-mA, N3-mA or N3-mC in mouse ES cells or Alkbh1 knockout cells by MS. d , LC-MS/MS analysis of N1-mA or N6-mA digested from synthetic oligonucleotides (top) and ES cell DNA samples (bottom). e , ESI-QTOF-MS/MS spectra of analytical standard of N6-mA nucleosides (top) and N6-mA containing HPLC fraction from ES cells.

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Knock-Out, High Performance Liquid Chromatography

7) Product Images from "The Scientific Basis and Advantage of Human Experiential Assessment in the quality control of Chinese Herbal Medicines exampling as Schisandrae Chinensis Fructus"

Article Title: The Scientific Basis and Advantage of Human Experiential Assessment in the quality control of Chinese Herbal Medicines exampling as Schisandrae Chinensis Fructus

Journal: Scientific Reports

doi: 10.1038/s41598-018-23619-5

HPLC chromatogram of SCF, the above one was sample solution, and the below one was mixed references solution ( A ). Content of lignans in different part of SCF ( B ). (This image was created by Dr. Zhou).
Figure Legend Snippet: HPLC chromatogram of SCF, the above one was sample solution, and the below one was mixed references solution ( A ). Content of lignans in different part of SCF ( B ). (This image was created by Dr. Zhou).

Techniques Used: High Performance Liquid Chromatography

8) Product Images from "Human Herpesvirus 8 infection may contribute to oxidative stress in diabetes type 2 patients"

Article Title: Human Herpesvirus 8 infection may contribute to oxidative stress in diabetes type 2 patients

Journal: BMC Research Notes

doi: 10.1186/s13104-020-4935-3

Lipid concentrations in control and DM2 subjects. a Unsaturated fatty acids (UFA) and b cholesterol were extracted from plasma samples, separated, identified and quantified by HPLC as reported in “ Materials and methods ” section. No differences in UFA or cholesterol were found between DM2 and controls, either HHV8-positive or -negative. The data are expressed as the mean concentration values + SEM and significance was calculated by ANOVA and Bonferroni post hoc tests. CTR non-diabetic control subjects, DM2 diabetic subjects, HHV8 infected subjects (patterned bars)
Figure Legend Snippet: Lipid concentrations in control and DM2 subjects. a Unsaturated fatty acids (UFA) and b cholesterol were extracted from plasma samples, separated, identified and quantified by HPLC as reported in “ Materials and methods ” section. No differences in UFA or cholesterol were found between DM2 and controls, either HHV8-positive or -negative. The data are expressed as the mean concentration values + SEM and significance was calculated by ANOVA and Bonferroni post hoc tests. CTR non-diabetic control subjects, DM2 diabetic subjects, HHV8 infected subjects (patterned bars)

Techniques Used: High Performance Liquid Chromatography, Concentration Assay, Infection

9) Product Images from "How does curcumin work with poor bioavailability? Clues from experimental and theoretical studies"

Article Title: How does curcumin work with poor bioavailability? Clues from experimental and theoretical studies

Journal: Scientific Reports

doi: 10.1038/srep20872

The HPLC analysis of curcumin and its degradation products mixture and the amplified figure. The samples were subjected to Agilent Extend-C18 column, with an eluting solution (MeOH: H 2 O: HAc = 75: 25: 0.5, 1.0 mL/min) and the detection wavelength of 430 nm.
Figure Legend Snippet: The HPLC analysis of curcumin and its degradation products mixture and the amplified figure. The samples were subjected to Agilent Extend-C18 column, with an eluting solution (MeOH: H 2 O: HAc = 75: 25: 0.5, 1.0 mL/min) and the detection wavelength of 430 nm.

Techniques Used: High Performance Liquid Chromatography, Amplification, HAC Assay

10) Product Images from "In Vitro and In Vivo Characterization of Selected Fluorine-18 Labeled Radioligands for PET Imaging of the Dopamine D3 Receptor"

Article Title: In Vitro and In Vivo Characterization of Selected Fluorine-18 Labeled Radioligands for PET Imaging of the Dopamine D3 Receptor

Journal: Molecules

doi: 10.3390/molecules21091144

Stability of radioligands [ 18 F]1 and [ 18 F]2b in blood of living rats under isoflurane anesthesia. ( a , c ) Radio-chromatogram obtained by HPLC of rat plasma extract taken 15 min after i.v. administration of the radioligand; ( b , d ) Fraction of intact tracer and radiometabolite as a function of time. Points represent the mean ± SEM of duplicates.
Figure Legend Snippet: Stability of radioligands [ 18 F]1 and [ 18 F]2b in blood of living rats under isoflurane anesthesia. ( a , c ) Radio-chromatogram obtained by HPLC of rat plasma extract taken 15 min after i.v. administration of the radioligand; ( b , d ) Fraction of intact tracer and radiometabolite as a function of time. Points represent the mean ± SEM of duplicates.

Techniques Used: High Performance Liquid Chromatography

11) Product Images from "DNA methylation on N6-adenine in mammalian embryonic stem cells"

Article Title: DNA methylation on N6-adenine in mammalian embryonic stem cells

Journal: Nature

doi: 10.1038/nature17640

LC-MS/MS data of N6-mA a N5]N6-mA was used as the internal standard. b , N6-mA levels are ultralow in adult tissues. c , No detection of DNA alkylation adducts, such as N1-mA, N3-mA or N3-mC in mouse ES cells or Alkbh1 knockout cells by MS. d , LC-MS/MS analysis of N1-mA or N6-mA digested from synthetic oligonucleotides (top) and ES cell DNA samples (bottom). e , ESI-QTOF-MS/MS spectra of analytical standard of N6-mA nucleosides (top) and N6-mA containing HPLC fraction from ES cells.
Figure Legend Snippet: LC-MS/MS data of N6-mA a N5]N6-mA was used as the internal standard. b , N6-mA levels are ultralow in adult tissues. c , No detection of DNA alkylation adducts, such as N1-mA, N3-mA or N3-mC in mouse ES cells or Alkbh1 knockout cells by MS. d , LC-MS/MS analysis of N1-mA or N6-mA digested from synthetic oligonucleotides (top) and ES cell DNA samples (bottom). e , ESI-QTOF-MS/MS spectra of analytical standard of N6-mA nucleosides (top) and N6-mA containing HPLC fraction from ES cells.

Techniques Used: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Knock-Out, High Performance Liquid Chromatography

12) Product Images from "Characterization and Pharmacological Evaluation of Anti-Cellulite Herbal Product(s) Encapsulated in 3D-Fabricated Polymeric Microneedles"

Article Title: Characterization and Pharmacological Evaluation of Anti-Cellulite Herbal Product(s) Encapsulated in 3D-Fabricated Polymeric Microneedles

Journal: Scientific Reports

doi: 10.1038/s41598-020-63271-6

HPLC chromatogram of the aqueous methanolic extract of ( A ) V.agnus-castus and ( B ) T.indica .
Figure Legend Snippet: HPLC chromatogram of the aqueous methanolic extract of ( A ) V.agnus-castus and ( B ) T.indica .

Techniques Used: High Performance Liquid Chromatography

13) Product Images from "Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage"

Article Title: Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage

Journal: PeerJ

doi: 10.7717/peerj.5326

HPLC chromatograms of the EPS samples and standards. (A) standards, (B) EPS-YO175, (C) EPS-OF101. The peaks correspond to glucose (peak 1), xylose (peak 2), galactose (peak 3), fructose (peak 4), rhamnose (peak 5).
Figure Legend Snippet: HPLC chromatograms of the EPS samples and standards. (A) standards, (B) EPS-YO175, (C) EPS-OF101. The peaks correspond to glucose (peak 1), xylose (peak 2), galactose (peak 3), fructose (peak 4), rhamnose (peak 5).

Techniques Used: High Performance Liquid Chromatography

14) Product Images from "Simultaneous production of laccase and degradation of bisphenol A with Trametes versicolor cultivated on agricultural wastes"

Article Title: Simultaneous production of laccase and degradation of bisphenol A with Trametes versicolor cultivated on agricultural wastes

Journal: Bioprocess and Biosystems Engineering

doi: 10.1007/s00449-017-1783-1

The HPLC chromatogram of BPA degradation with T. versicolor . Culture time: a 0 days; b 10 days
Figure Legend Snippet: The HPLC chromatogram of BPA degradation with T. versicolor . Culture time: a 0 days; b 10 days

Techniques Used: High Performance Liquid Chromatography

15) Product Images from "Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups"

Article Title: Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2018.01840

Trans -acylation reaction assays of various AT4 FkbB mutants after substitution of Val187 with Asp, or Cys, Ile, Lys, Trp, and Phe with allmal- and ethmal-CoA as substrates and the Production of FK506 and FK520 in WT, YN06-01, and YN06-02. (A) The change of transferring allmal/ethmal unit to ACP10 FkbA in trans -acylation reactions by AT4 FkbB and its mutants V187D, V187C, V187I, V187K, V187W, and V187F. HPLC analyses of transferring allmal-CoA : ethmal-CoA = 1:1 to holo-ACP10 FkbA in the presence of V187K (B) and in the absence of AT4 FkbB (D) . MS analyses of transferring allmal-CoA : ethmal-CoA = 1:1 to holo-ACP10 FkbA in the presence of V187K (C) and in the absence of AT4 FkbB (E) . The peaks were assigned as follows: b 0 , holo-ACP10 FkbA ; b 1 , allmal-ACP10 FkbA ; b 2 , ethmal-ACP10 FkbA . (F) Production of FK506 and FK520 in WT, YN06-01 and YN06-02. Analyses of variance were conducted to determine the difference of WT and mutants using SPSS 20. The LSD multiple range tests were evaluated for significant differences among WT and mutants ( P
Figure Legend Snippet: Trans -acylation reaction assays of various AT4 FkbB mutants after substitution of Val187 with Asp, or Cys, Ile, Lys, Trp, and Phe with allmal- and ethmal-CoA as substrates and the Production of FK506 and FK520 in WT, YN06-01, and YN06-02. (A) The change of transferring allmal/ethmal unit to ACP10 FkbA in trans -acylation reactions by AT4 FkbB and its mutants V187D, V187C, V187I, V187K, V187W, and V187F. HPLC analyses of transferring allmal-CoA : ethmal-CoA = 1:1 to holo-ACP10 FkbA in the presence of V187K (B) and in the absence of AT4 FkbB (D) . MS analyses of transferring allmal-CoA : ethmal-CoA = 1:1 to holo-ACP10 FkbA in the presence of V187K (C) and in the absence of AT4 FkbB (E) . The peaks were assigned as follows: b 0 , holo-ACP10 FkbA ; b 1 , allmal-ACP10 FkbA ; b 2 , ethmal-ACP10 FkbA . (F) Production of FK506 and FK520 in WT, YN06-01 and YN06-02. Analyses of variance were conducted to determine the difference of WT and mutants using SPSS 20. The LSD multiple range tests were evaluated for significant differences among WT and mutants ( P

Techniques Used: Transferring, High Performance Liquid Chromatography, Mass Spectrometry

16) Product Images from "Mass-Based Fraction Collection of Crude Synthetic Peptides in Analytical and Preparative Scale"

Article Title: Mass-Based Fraction Collection of Crude Synthetic Peptides in Analytical and Preparative Scale

Journal: Journal of Biomolecular Techniques : JBT

doi:

Instrumental setup for mass-based fraction collection. The system comprises two flow paths. One flow path, the main flow, leads from the HPLC system to the Agilent active splitter and finally to the fraction collector. The second flow path, the make-up flow, leads from the make-up pump to the Agilent active splitter and finally to the MSD. For on-line mass detection, the active splitter transfers small aliquots from the main flow to the make-up flow.
Figure Legend Snippet: Instrumental setup for mass-based fraction collection. The system comprises two flow paths. One flow path, the main flow, leads from the HPLC system to the Agilent active splitter and finally to the fraction collector. The second flow path, the make-up flow, leads from the make-up pump to the Agilent active splitter and finally to the MSD. For on-line mass detection, the active splitter transfers small aliquots from the main flow to the make-up flow.

Techniques Used: Flow Cytometry, High Performance Liquid Chromatography

17) Product Images from "An L213A variant of β-glycosidase from Sulfolobus solfataricus with increased α-L-arabinofuranosidase activity converts ginsenoside Rc to compound K"

Article Title: An L213A variant of β-glycosidase from Sulfolobus solfataricus with increased α-L-arabinofuranosidase activity converts ginsenoside Rc to compound K

Journal: PLoS ONE

doi: 10.1371/journal.pone.0191018

HPLC profiles of the reaction solutions obtained after 4 h and 10 h for the production of C-K from ginsenoside Rc by (A) the wild-type and (B) L213A variant β - glycosidases from S . solfataricus .
Figure Legend Snippet: HPLC profiles of the reaction solutions obtained after 4 h and 10 h for the production of C-K from ginsenoside Rc by (A) the wild-type and (B) L213A variant β - glycosidases from S . solfataricus .

Techniques Used: High Performance Liquid Chromatography, Variant Assay

HPLC profiles of the reaction solutions obtained after 14 h for the production of C-K from ginsenoside Mc by (A) the wild-type and (B) L213A variant enzymes from S . solfataricus .
Figure Legend Snippet: HPLC profiles of the reaction solutions obtained after 14 h for the production of C-K from ginsenoside Mc by (A) the wild-type and (B) L213A variant enzymes from S . solfataricus .

Techniques Used: High Performance Liquid Chromatography, Variant Assay

18) Product Images from "Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage"

Article Title: Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage

Journal: PeerJ

doi: 10.7717/peerj.5326

HPLC chromatograms of the EPS samples and standards. (A) standards, (B) EPS-YO175, (C) EPS-OF101. The peaks correspond to glucose (peak 1), xylose (peak 2), galactose (peak 3), fructose (peak 4), rhamnose (peak 5).
Figure Legend Snippet: HPLC chromatograms of the EPS samples and standards. (A) standards, (B) EPS-YO175, (C) EPS-OF101. The peaks correspond to glucose (peak 1), xylose (peak 2), galactose (peak 3), fructose (peak 4), rhamnose (peak 5).

Techniques Used: High Performance Liquid Chromatography

19) Product Images from "(−)Doxazosin is a necessary component for the hypotensive effect of (±)doxazosin during long-term administration in conscious rats"

Article Title: (−)Doxazosin is a necessary component for the hypotensive effect of (±)doxazosin during long-term administration in conscious rats

Journal: Acta Pharmacologica Sinica

doi: 10.1038/aps.2013.154

Typical HPLC chromatograms obtained from a blank plasma sample determined using an achiral column (A); a rat plasma sample collected after the oral administration of a racemic doxazosin and determined using an achiral column (B); a blank plasma sample determined using a chiral column (C); the rat plasma sample aliquoted from that for (B) and determined by a chiral column (D). Peaks: 1) prazosin, 2) doxazosin, 3) (−)doxazosin, and 4) (+)doxazosin. For more experimental details, refer to the Materials and methods section.
Figure Legend Snippet: Typical HPLC chromatograms obtained from a blank plasma sample determined using an achiral column (A); a rat plasma sample collected after the oral administration of a racemic doxazosin and determined using an achiral column (B); a blank plasma sample determined using a chiral column (C); the rat plasma sample aliquoted from that for (B) and determined by a chiral column (D). Peaks: 1) prazosin, 2) doxazosin, 3) (−)doxazosin, and 4) (+)doxazosin. For more experimental details, refer to the Materials and methods section.

Techniques Used: High Performance Liquid Chromatography

20) Product Images from "(−)Doxazosin is a necessary component for the hypotensive effect of (±)doxazosin during long-term administration in conscious rats"

Article Title: (−)Doxazosin is a necessary component for the hypotensive effect of (±)doxazosin during long-term administration in conscious rats

Journal: Acta Pharmacologica Sinica

doi: 10.1038/aps.2013.154

Typical HPLC chromatograms obtained from a blank plasma sample determined using an achiral column (A); a rat plasma sample collected after the oral administration of a racemic doxazosin and determined using an achiral column (B); a blank plasma sample determined using a chiral column (C); the rat plasma sample aliquoted from that for (B) and determined by a chiral column (D). Peaks: 1) prazosin, 2) doxazosin, 3) (−)doxazosin, and 4) (+)doxazosin. For more experimental details, refer to the Materials and methods section.
Figure Legend Snippet: Typical HPLC chromatograms obtained from a blank plasma sample determined using an achiral column (A); a rat plasma sample collected after the oral administration of a racemic doxazosin and determined using an achiral column (B); a blank plasma sample determined using a chiral column (C); the rat plasma sample aliquoted from that for (B) and determined by a chiral column (D). Peaks: 1) prazosin, 2) doxazosin, 3) (−)doxazosin, and 4) (+)doxazosin. For more experimental details, refer to the Materials and methods section.

Techniques Used: High Performance Liquid Chromatography

21) Product Images from "The development of a GPR44 targeting radioligand [11C]AZ12204657 for in vivo assessment of beta cell mass"

Article Title: The development of a GPR44 targeting radioligand [11C]AZ12204657 for in vivo assessment of beta cell mass

Journal: EJNMMI Research

doi: 10.1186/s13550-018-0465-6

a HPLC Chromatograms of the semi-preparative purification, R t ([ 11 CAZ12204657) = 7–9 min. The lower trace shows radioactivity response, and the upper trace, absorbance response. The mobile phase system is CH 3 CN/0.1 M AF/sodium- l -ascorbate (35/65/0.5, v/v/w ) at a flow rate of 4 mL/min. b The analytical HPLC chromatogram of co-injection of standard and radiolabeled [ 11 C]AZ12204657; R t = 4–6 min. The mobile phase system is CH 3 CN/0.1 M AF/sodium- l -Ascorbate (25/75/0.5, v/v/w ) at a flow rate of 3 mL/min
Figure Legend Snippet: a HPLC Chromatograms of the semi-preparative purification, R t ([ 11 CAZ12204657) = 7–9 min. The lower trace shows radioactivity response, and the upper trace, absorbance response. The mobile phase system is CH 3 CN/0.1 M AF/sodium- l -ascorbate (35/65/0.5, v/v/w ) at a flow rate of 4 mL/min. b The analytical HPLC chromatogram of co-injection of standard and radiolabeled [ 11 C]AZ12204657; R t = 4–6 min. The mobile phase system is CH 3 CN/0.1 M AF/sodium- l -Ascorbate (25/75/0.5, v/v/w ) at a flow rate of 3 mL/min

Techniques Used: High Performance Liquid Chromatography, Purification, Radioactivity, Flow Cytometry, Injection

22) Product Images from "In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes"

Article Title: In vitro safety assessment of the strawberry tree (Arbutus unedo L.) water leaf extract and arbutin in human peripheral blood lymphocytes

Journal: Cytotechnology

doi: 10.1007/s10616-018-0218-4

HPLC–DAD chromatogram of the Arbutus unedo L. water leaf extract. The extract contained 10.7 mg arbutin per g of lyophilisate
Figure Legend Snippet: HPLC–DAD chromatogram of the Arbutus unedo L. water leaf extract. The extract contained 10.7 mg arbutin per g of lyophilisate

Techniques Used: High Performance Liquid Chromatography

23) Product Images from "Two-dimensional high performance liquid chromatography separation and tandem mass spectrometry detection of atrazine and its metabolic and hydrolysis products in urine"

Article Title: Two-dimensional high performance liquid chromatography separation and tandem mass spectrometry detection of atrazine and its metabolic and hydrolysis products in urine

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

doi: 10.1016/j.jchromb.2012.05.028

MS/MS chromatograms collected from a 50 μg/L spiked urine sample by connecting the mass spectrometer after the SCX-t SPE column without analytical columns (A), and by connecting the MS instrument after the analytical columns with the final 2D-HPLC–MS/MS
Figure Legend Snippet: MS/MS chromatograms collected from a 50 μg/L spiked urine sample by connecting the mass spectrometer after the SCX-t SPE column without analytical columns (A), and by connecting the MS instrument after the analytical columns with the final 2D-HPLC–MS/MS

Techniques Used: Mass Spectrometry, High Performance Liquid Chromatography

24) Product Images from "Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis"

Article Title: Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis

Journal: Scientific Reports

doi: 10.1038/s41598-017-16653-2

( a ) In vitro cleavage of Apo-S-Ac 3 ManNAz was monitored using HPLC system at 0, 3, 6, and 24 h post-incubation with Cas-3 (15 μg/ml). ( b ) As a control experiment, Apo-S-Ac 3 ManNAz was also monitored using HPLC system at 24 h post-incubation with 15 μg/ml of Cas-1, Cas-8, Cathepsin B and MMP-9, respectively. The generation of azido groups on the surface of non-apoptotic PC-3 tumor cells in vitro . ( c ) Time-dependent CLSM images of Ac 3 ManNAz (20 μM) or Apo-S-Ac 3 ManNAz (20 μM)-treated PC-3 tumor cells, followed by DBCO-Cy5.5 (200 nM) to visualize azido groups on the cell surface. Red = DBCO-Cy5.5 channel; Blue = DAPI channel. ( d ) Western blot analysis of azido groups (N 3 ) containing glycoproteins in cell lysates. The coomassie blue (CB) stain shows the total amount of glycoproteins in cell lysates. ( e ) Quantification of band intensity of azido groups of Western blot data using ImageJ software.
Figure Legend Snippet: ( a ) In vitro cleavage of Apo-S-Ac 3 ManNAz was monitored using HPLC system at 0, 3, 6, and 24 h post-incubation with Cas-3 (15 μg/ml). ( b ) As a control experiment, Apo-S-Ac 3 ManNAz was also monitored using HPLC system at 24 h post-incubation with 15 μg/ml of Cas-1, Cas-8, Cathepsin B and MMP-9, respectively. The generation of azido groups on the surface of non-apoptotic PC-3 tumor cells in vitro . ( c ) Time-dependent CLSM images of Ac 3 ManNAz (20 μM) or Apo-S-Ac 3 ManNAz (20 μM)-treated PC-3 tumor cells, followed by DBCO-Cy5.5 (200 nM) to visualize azido groups on the cell surface. Red = DBCO-Cy5.5 channel; Blue = DAPI channel. ( d ) Western blot analysis of azido groups (N 3 ) containing glycoproteins in cell lysates. The coomassie blue (CB) stain shows the total amount of glycoproteins in cell lysates. ( e ) Quantification of band intensity of azido groups of Western blot data using ImageJ software.

Techniques Used: In Vitro, High Performance Liquid Chromatography, Incubation, Confocal Laser Scanning Microscopy, Western Blot, Staining, Software

25) Product Images from "Pharmacokinetics and pharmacodynamics of linezolid in plasma/cerebrospinal fluid in patients with cerebral hemorrhage after lateral ventricular drainage by Monte Carlo simulation"

Article Title: Pharmacokinetics and pharmacodynamics of linezolid in plasma/cerebrospinal fluid in patients with cerebral hemorrhage after lateral ventricular drainage by Monte Carlo simulation

Journal: Drug Design, Development and Therapy

doi: 10.2147/DDDT.S168757

HPLC chromatogram of linezolid from CSF at 3 h after the infusion. Abbreviations: HPLC, high-performance liquid chromatography; CSF, cerebrospinal fluid.
Figure Legend Snippet: HPLC chromatogram of linezolid from CSF at 3 h after the infusion. Abbreviations: HPLC, high-performance liquid chromatography; CSF, cerebrospinal fluid.

Techniques Used: High Performance Liquid Chromatography

26) Product Images from "Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups"

Article Title: Substrate Specificity of Acyltransferase Domains for Efficient Transfer of Acyl Groups

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2018.01840

Trans -acylation reaction assays of various AT4 FkbB mutants after substitution of Val187 with Asp, or Cys, Ile, Lys, Trp, and Phe with allmal- and ethmal-CoA as substrates and the Production of FK506 and FK520 in WT, YN06-01, and YN06-02. (A) The change of transferring allmal/ethmal unit to ACP10 FkbA in trans -acylation reactions by AT4 FkbB and its mutants V187D, V187C, V187I, V187K, V187W, and V187F. HPLC analyses of transferring allmal-CoA : ethmal-CoA = 1:1 to holo-ACP10 FkbA in the presence of V187K (B) and in the absence of AT4 FkbB (D) . MS analyses of transferring allmal-CoA : ethmal-CoA = 1:1 to holo-ACP10 FkbA in the presence of V187K (C) and in the absence of AT4 FkbB (E) . The peaks were assigned as follows: b 0 , holo-ACP10 FkbA ; b 1 , allmal-ACP10 FkbA ; b 2 , ethmal-ACP10 FkbA . (F) Production of FK506 and FK520 in WT, YN06-01 and YN06-02. Analyses of variance were conducted to determine the difference of WT and mutants using SPSS 20. The LSD multiple range tests were evaluated for significant differences among WT and mutants ( P
Figure Legend Snippet: Trans -acylation reaction assays of various AT4 FkbB mutants after substitution of Val187 with Asp, or Cys, Ile, Lys, Trp, and Phe with allmal- and ethmal-CoA as substrates and the Production of FK506 and FK520 in WT, YN06-01, and YN06-02. (A) The change of transferring allmal/ethmal unit to ACP10 FkbA in trans -acylation reactions by AT4 FkbB and its mutants V187D, V187C, V187I, V187K, V187W, and V187F. HPLC analyses of transferring allmal-CoA : ethmal-CoA = 1:1 to holo-ACP10 FkbA in the presence of V187K (B) and in the absence of AT4 FkbB (D) . MS analyses of transferring allmal-CoA : ethmal-CoA = 1:1 to holo-ACP10 FkbA in the presence of V187K (C) and in the absence of AT4 FkbB (E) . The peaks were assigned as follows: b 0 , holo-ACP10 FkbA ; b 1 , allmal-ACP10 FkbA ; b 2 , ethmal-ACP10 FkbA . (F) Production of FK506 and FK520 in WT, YN06-01 and YN06-02. Analyses of variance were conducted to determine the difference of WT and mutants using SPSS 20. The LSD multiple range tests were evaluated for significant differences among WT and mutants ( P

Techniques Used: Transferring, High Performance Liquid Chromatography, Mass Spectrometry

27) Product Images from "An L213A variant of β-glycosidase from Sulfolobus solfataricus with increased α-L-arabinofuranosidase activity converts ginsenoside Rc to compound K"

Article Title: An L213A variant of β-glycosidase from Sulfolobus solfataricus with increased α-L-arabinofuranosidase activity converts ginsenoside Rc to compound K

Journal: PLoS ONE

doi: 10.1371/journal.pone.0191018

HPLC profiles of the reaction solutions obtained after 4 h and 10 h for the production of C-K from ginsenoside Rc by (A) the wild-type and (B) L213A variant β - glycosidases from S . solfataricus .
Figure Legend Snippet: HPLC profiles of the reaction solutions obtained after 4 h and 10 h for the production of C-K from ginsenoside Rc by (A) the wild-type and (B) L213A variant β - glycosidases from S . solfataricus .

Techniques Used: High Performance Liquid Chromatography, Variant Assay

HPLC profiles of the reaction solutions obtained after 14 h for the production of C-K from ginsenoside Mc by (A) the wild-type and (B) L213A variant enzymes from S . solfataricus .
Figure Legend Snippet: HPLC profiles of the reaction solutions obtained after 14 h for the production of C-K from ginsenoside Mc by (A) the wild-type and (B) L213A variant enzymes from S . solfataricus .

Techniques Used: High Performance Liquid Chromatography, Variant Assay

28) Product Images from "Pharmacokinetics and pharmacodynamics of linezolid in plasma/cerebrospinal fluid in patients with cerebral hemorrhage after lateral ventricular drainage by Monte Carlo simulation"

Article Title: Pharmacokinetics and pharmacodynamics of linezolid in plasma/cerebrospinal fluid in patients with cerebral hemorrhage after lateral ventricular drainage by Monte Carlo simulation

Journal: Drug Design, Development and Therapy

doi: 10.2147/DDDT.S168757

HPLC chromatogram of linezolid from CSF at 3 h after the infusion. Abbreviations: HPLC, high-performance liquid chromatography; CSF, cerebrospinal fluid.
Figure Legend Snippet: HPLC chromatogram of linezolid from CSF at 3 h after the infusion. Abbreviations: HPLC, high-performance liquid chromatography; CSF, cerebrospinal fluid.

Techniques Used: High Performance Liquid Chromatography

29) Product Images from "Automated production of [18F]VAT suitable for clinical PET study of vesicular acetylcholine transporter"

Article Title: Automated production of [18F]VAT suitable for clinical PET study of vesicular acetylcholine transporter

Journal: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine

doi: 10.1016/j.apradiso.2015.09.010

Chromatograms of representative semi-preparative HPLC purification of [ 18 F]VAT. Agilent Zorbax SB-C18 column, 38% acetonitrile in 62% 0.1 M ammonium formate, v/v, pH 6.5, 4 mL/min. Panel A: radiochemical trace and panel B: UV trace at 254 nm.
Figure Legend Snippet: Chromatograms of representative semi-preparative HPLC purification of [ 18 F]VAT. Agilent Zorbax SB-C18 column, 38% acetonitrile in 62% 0.1 M ammonium formate, v/v, pH 6.5, 4 mL/min. Panel A: radiochemical trace and panel B: UV trace at 254 nm.

Techniques Used: High Performance Liquid Chromatography, Purification

Chromatograms of representative semi-preparative HPLC purification of 2-[ 18 F]fluoroethyl tosylate. Agilent Zorbax SB-C18 column, 50% acetonitrile in 50% 0.1 M ammonium formate, v/v, pH 6.5, 5 mL/min. Panel A: radiochemical trace and panel B: UV trace
Figure Legend Snippet: Chromatograms of representative semi-preparative HPLC purification of 2-[ 18 F]fluoroethyl tosylate. Agilent Zorbax SB-C18 column, 50% acetonitrile in 50% 0.1 M ammonium formate, v/v, pH 6.5, 5 mL/min. Panel A: radiochemical trace and panel B: UV trace

Techniques Used: High Performance Liquid Chromatography, Purification

30) Product Images from "(−)Doxazosin is a necessary component for the hypotensive effect of (±)doxazosin during long-term administration in conscious rats"

Article Title: (−)Doxazosin is a necessary component for the hypotensive effect of (±)doxazosin during long-term administration in conscious rats

Journal: Acta Pharmacologica Sinica

doi: 10.1038/aps.2013.154

Typical HPLC chromatograms obtained from a blank plasma sample determined using an achiral column (A); a rat plasma sample collected after the oral administration of a racemic doxazosin and determined using an achiral column (B); a blank plasma sample determined using a chiral column (C); the rat plasma sample aliquoted from that for (B) and determined by a chiral column (D). Peaks: 1) prazosin, 2) doxazosin, 3) (−)doxazosin, and 4) (+)doxazosin. For more experimental details, refer to the Materials and methods section.
Figure Legend Snippet: Typical HPLC chromatograms obtained from a blank plasma sample determined using an achiral column (A); a rat plasma sample collected after the oral administration of a racemic doxazosin and determined using an achiral column (B); a blank plasma sample determined using a chiral column (C); the rat plasma sample aliquoted from that for (B) and determined by a chiral column (D). Peaks: 1) prazosin, 2) doxazosin, 3) (−)doxazosin, and 4) (+)doxazosin. For more experimental details, refer to the Materials and methods section.

Techniques Used: High Performance Liquid Chromatography

31) Product Images from "Annona muricata Leaf Extract Triggered Intrinsic Apoptotic Pathway to Attenuate Cancerous Features of Triple Negative Breast Cancer MDA-MB-231 Cells"

Article Title: Annona muricata Leaf Extract Triggered Intrinsic Apoptotic Pathway to Attenuate Cancerous Features of Triple Negative Breast Cancer MDA-MB-231 Cells

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

doi: 10.1155/2018/7972916

The extracted-ion chromatograms obtained for the A. muricata leaf extract using HPLC-QTOF . (a) EIC of m/z 595.4574 (± 10.0 ppm) [C 35 H 62 O 7 +H]+; (b) EIC of m/z 597.4730 (± 10.0 ppm) [C 35 H 64 O 7 +H]+; (c) EIC of m/z 613.4679 (± 10.0 ppm) [C 35 H 64 O 8 +H]+; (d) EIC of m/z 629.4629 (± 10.0 ppm) [C 35 H 64 O 9 +H]+.
Figure Legend Snippet: The extracted-ion chromatograms obtained for the A. muricata leaf extract using HPLC-QTOF . (a) EIC of m/z 595.4574 (± 10.0 ppm) [C 35 H 62 O 7 +H]+; (b) EIC of m/z 597.4730 (± 10.0 ppm) [C 35 H 64 O 7 +H]+; (c) EIC of m/z 613.4679 (± 10.0 ppm) [C 35 H 64 O 8 +H]+; (d) EIC of m/z 629.4629 (± 10.0 ppm) [C 35 H 64 O 9 +H]+.

Techniques Used: High Performance Liquid Chromatography

32) Product Images from "The Ameliorating Effect of Steamed and Fermented Codonopsis lanceolata on Scopolamine-Induced Memory Impairment in Mice"

Article Title: The Ameliorating Effect of Steamed and Fermented Codonopsis lanceolata on Scopolamine-Induced Memory Impairment in Mice

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

doi: 10.1155/2013/464576

HPLC chromatogram of six phenolic standard compounds (a) and fermented C. lanceolata extract (b). GA: gallic acid; CA: caffeic acid; VA: vanillic acid; 4-CA: 4-coumaric acid; t-FA: trans-ferulic acid; C: caffeine.
Figure Legend Snippet: HPLC chromatogram of six phenolic standard compounds (a) and fermented C. lanceolata extract (b). GA: gallic acid; CA: caffeic acid; VA: vanillic acid; 4-CA: 4-coumaric acid; t-FA: trans-ferulic acid; C: caffeine.

Techniques Used: High Performance Liquid Chromatography

33) Product Images from "Cloning, Functional Characterization and Site-Directed Mutagenesis of 4-Coumarate: Coenzyme A Ligase (4CL) Involved in Coumarin Biosynthesis in Peucedanum praeruptorum Dunn"

Article Title: Cloning, Functional Characterization and Site-Directed Mutagenesis of 4-Coumarate: Coenzyme A Ligase (4CL) Involved in Coumarin Biosynthesis in Peucedanum praeruptorum Dunn

Journal: Frontiers in Plant Science

doi: 10.3389/fpls.2017.00004

Substrate specificity of recombinant Pp4CL1. HPLC analysis and Q-TOF-MS identification of reaction products generated by recombinant Pp4CL1 protein. (A) p -Coumaric acid as substrate. (B) Ferulic acid as substrate. (C) Caffeic acid as substrate. (D) Isoferulic acid as substrate. (E) o -Coumaric acid as substrate. (F) Cinnamic acid as substrate.
Figure Legend Snippet: Substrate specificity of recombinant Pp4CL1. HPLC analysis and Q-TOF-MS identification of reaction products generated by recombinant Pp4CL1 protein. (A) p -Coumaric acid as substrate. (B) Ferulic acid as substrate. (C) Caffeic acid as substrate. (D) Isoferulic acid as substrate. (E) o -Coumaric acid as substrate. (F) Cinnamic acid as substrate.

Techniques Used: Recombinant, High Performance Liquid Chromatography, Mass Spectrometry, Generated

34) Product Images from "Electroporation-enhanced transdermal diclofenac sodium delivery into the knee joint in a rat model of acute arthritis"

Article Title: Electroporation-enhanced transdermal diclofenac sodium delivery into the knee joint in a rat model of acute arthritis

Journal: Drug Design, Development and Therapy

doi: 10.2147/DDDT.S161703

Diclofenac concentrations in the serum (black columns) and the synovial washing fluid (gray columns) measured by HPLC (Series 1). Notes: Diclofenac sodium gel was applied topically above the knee joint (topical), or EP was applied for 8 min over the knee joint (EP) after the diclofenac gel dispersed. Samples of blood and synovial washing fluid were collected 10 min after application. Data are presented as means ± SD. # p
Figure Legend Snippet: Diclofenac concentrations in the serum (black columns) and the synovial washing fluid (gray columns) measured by HPLC (Series 1). Notes: Diclofenac sodium gel was applied topically above the knee joint (topical), or EP was applied for 8 min over the knee joint (EP) after the diclofenac gel dispersed. Samples of blood and synovial washing fluid were collected 10 min after application. Data are presented as means ± SD. # p

Techniques Used: High Performance Liquid Chromatography

Time sequence of interventions. Notes: In the first series, the diclofenac concentration in the serum and the synovial washing fluid was measured by HPLC. In the second experimental series, tests for secondary mechanical touch sensitivity and heat-provoked paw withdrawal were performed 24 h after arthritis induction with C/K and for knee joint swelling measurements 48 h after the challenge. In the third series, IVM examinations of the synovial membrane were performed 6 h after the challenge. Abbreviations: C/K, carrageenan/kaolin; D, diclofenac treatment; HPLC, high-performance liquid chromatography; IVM, intravital videomicroscopy; S, sample taken.
Figure Legend Snippet: Time sequence of interventions. Notes: In the first series, the diclofenac concentration in the serum and the synovial washing fluid was measured by HPLC. In the second experimental series, tests for secondary mechanical touch sensitivity and heat-provoked paw withdrawal were performed 24 h after arthritis induction with C/K and for knee joint swelling measurements 48 h after the challenge. In the third series, IVM examinations of the synovial membrane were performed 6 h after the challenge. Abbreviations: C/K, carrageenan/kaolin; D, diclofenac treatment; HPLC, high-performance liquid chromatography; IVM, intravital videomicroscopy; S, sample taken.

Techniques Used: Sequencing, Concentration Assay, High Performance Liquid Chromatography

35) Product Images from "No synergism between bis(propyl)-cognitin and rasagiline on protecting dopaminergic neurons in Parkinson's disease mice"

Article Title: No synergism between bis(propyl)-cognitin and rasagiline on protecting dopaminergic neurons in Parkinson's disease mice

Journal: Neural Regeneration Research

doi: 10.4103/1673-5374.189201

B3C, rasagiline and their combination reduced MPTP-induced decrease of dopamine and its metabolites in mice. (A) Representative chromatographic profiles of dopamine, DOPAC and HVA detected by ECD-HPLC. At 15 th day after MPTP injection, (B) the content of striatal dopamine, (C) the content of striatal DOPAC, and (D) the content of striatal HVA were analyzed by ECD-HPLC. All data are expressed as the mean ± SEM; n = 8 mice/group. ## P
Figure Legend Snippet: B3C, rasagiline and their combination reduced MPTP-induced decrease of dopamine and its metabolites in mice. (A) Representative chromatographic profiles of dopamine, DOPAC and HVA detected by ECD-HPLC. At 15 th day after MPTP injection, (B) the content of striatal dopamine, (C) the content of striatal DOPAC, and (D) the content of striatal HVA were analyzed by ECD-HPLC. All data are expressed as the mean ± SEM; n = 8 mice/group. ## P

Techniques Used: Mouse Assay, High Performance Liquid Chromatography, Injection

36) Product Images from "Metal-free ribonucleotide reduction powered by a DOPA radical in Mycoplasma pathogens"

Article Title: Metal-free ribonucleotide reduction powered by a DOPA radical in Mycoplasma pathogens

Journal: Nature

doi: 10.1038/s41586-018-0653-6

Metal analysis and radical generation in the presence of chelator. a) Representative TXRF spectra measured for Mf R2 (blue, at 664 μM) and Fe-reconstituted class Ia Ec R2 (orange, at 635 μM), on the 5 to 10 keV energy range. The spectra have been scaled using the peak size of the Ga internal standard and offset slightly in the Y-direction for clarity. K-level X-ray emission lines are indicated with arrows. For elements, where both Kα and Kβ lines are present, they are specified. Otherwise, arrows indicate Kα lines. Experiments were repeated three times. b) Concentrations of Mn, Fe, Co, Ni, Cu and Zn were measured in the active Mf R2, Mf NrdI and Mf R1 protein solutions and in their respective buffers, as well as in a solution of E. coli class Ia R2 protein reconstituted with Fe in vitro . Mean concentrations and SD of measurements on 3 independently prepared samples for each sample are reported. The concentrations were converted to metal to protein molar ratio. The measurements show that none of the Mf RNR proteins contain a significant amount of metal as opposed to Ec R2a which as expected contains on the order of 2 metal ions per monomer also after a desalting step. Buffer 1 is the buffer system used for Mf R2, i.e. 25 mM HEPES-Na pH 7, 50 mM NaCl. Buffer 2 is the buffer system used for Mf R1 and Mf NrdI, i.e. 25 mM Tris-HCl pH 8, 50 mM NaCl. The protein purification involves a Ni-affinity step which is likely the reason for nickel being the dominating metal species in the sample. c) HPLC based in vitro assays show that RNR activity can be restored after Mf R2 is quenched by hydroxyurea. Mf R2 is regenerated by the addition of Mf NrdI followed by redox cycling with dithionite and oxygen containing buffer (green) (see main ). Reactivation and activity are observed also in the presence of a metal chelator (EDTA 0.3 mM, blue). Addition of extra metals (0.2 mM of each Mn, Fe, Co, Ni, Cu, Zn) does not improve the activity recovery (pink). Fig. 2d
Figure Legend Snippet: Metal analysis and radical generation in the presence of chelator. a) Representative TXRF spectra measured for Mf R2 (blue, at 664 μM) and Fe-reconstituted class Ia Ec R2 (orange, at 635 μM), on the 5 to 10 keV energy range. The spectra have been scaled using the peak size of the Ga internal standard and offset slightly in the Y-direction for clarity. K-level X-ray emission lines are indicated with arrows. For elements, where both Kα and Kβ lines are present, they are specified. Otherwise, arrows indicate Kα lines. Experiments were repeated three times. b) Concentrations of Mn, Fe, Co, Ni, Cu and Zn were measured in the active Mf R2, Mf NrdI and Mf R1 protein solutions and in their respective buffers, as well as in a solution of E. coli class Ia R2 protein reconstituted with Fe in vitro . Mean concentrations and SD of measurements on 3 independently prepared samples for each sample are reported. The concentrations were converted to metal to protein molar ratio. The measurements show that none of the Mf RNR proteins contain a significant amount of metal as opposed to Ec R2a which as expected contains on the order of 2 metal ions per monomer also after a desalting step. Buffer 1 is the buffer system used for Mf R2, i.e. 25 mM HEPES-Na pH 7, 50 mM NaCl. Buffer 2 is the buffer system used for Mf R1 and Mf NrdI, i.e. 25 mM Tris-HCl pH 8, 50 mM NaCl. The protein purification involves a Ni-affinity step which is likely the reason for nickel being the dominating metal species in the sample. c) HPLC based in vitro assays show that RNR activity can be restored after Mf R2 is quenched by hydroxyurea. Mf R2 is regenerated by the addition of Mf NrdI followed by redox cycling with dithionite and oxygen containing buffer (green) (see main ). Reactivation and activity are observed also in the presence of a metal chelator (EDTA 0.3 mM, blue). Addition of extra metals (0.2 mM of each Mn, Fe, Co, Ni, Cu, Zn) does not improve the activity recovery (pink). Fig. 2d

Techniques Used: IA, In Vitro, Protein Purification, High Performance Liquid Chromatography, Activity Assay

A new RNR subclass able to rescue an Escherichia coli strain lacking aerobic RNR. a) Sequence alignment of the new R2 protein groups to a number of standard, di-metal containing, R2 proteins. Purple background indicates the 6 normally essential metal-binding residues, only 3 of which are conserved. Two variants are observed in which 3 carboxylate metal ligands are either substituted for valine, proline and lysine (VPK variant) or for glutamine, serine and lysine (QSK variant). The normally radical harboring tyrosine residue is shown with a green background. b) Taxonomic distribution of NrdF2. QSK/VPK encoding organisms and their collected RNR class repertoire. As common for class I RNRs, several genomes encoding the QSK or VPK variant also harbor other RNRs. The QSK clusters are found only in Actinobacteria, whereas the VPK clusters are also found in Firmicutes, Tenericutes, Chlamydiae and Fusobacteria. c) Expression of the MfnrdFIE operon induced by addition of 0.1% v/v L-arabinose (green) rescued the JEM164 double knockout (Δ nrdAB , Δ nrdEF ) strain while when gene expression was suppressed with 0.1% v/v D-glucose (brown) the strain failed to recover, as did the strain lacking the vector (red). Growth curves (average of 3 experiments with SD) are shown. d) Mf NrdI activates Mf R2 in an O 2 dependent reaction. HPLC based in vitro RNR activity assays show no activity for R2 protein expressed separately in E. coli (red), while aerobic co-expression with MfnrdI and MfnrdE (green) or MfnrdI (orange) produced an active R2 protein. Anaerobic co-expression (yellow) or incubation of the active R2 with hydroxyurea (light blue) abolishes the activity. Partial activity could be regenerated by the addition of Mf NrdI and redox cycling with dithionite and oxygen, blue and maroon for one and two reduction-oxidation cycles respectively. Data points are shown for triplicate experiments.
Figure Legend Snippet: A new RNR subclass able to rescue an Escherichia coli strain lacking aerobic RNR. a) Sequence alignment of the new R2 protein groups to a number of standard, di-metal containing, R2 proteins. Purple background indicates the 6 normally essential metal-binding residues, only 3 of which are conserved. Two variants are observed in which 3 carboxylate metal ligands are either substituted for valine, proline and lysine (VPK variant) or for glutamine, serine and lysine (QSK variant). The normally radical harboring tyrosine residue is shown with a green background. b) Taxonomic distribution of NrdF2. QSK/VPK encoding organisms and their collected RNR class repertoire. As common for class I RNRs, several genomes encoding the QSK or VPK variant also harbor other RNRs. The QSK clusters are found only in Actinobacteria, whereas the VPK clusters are also found in Firmicutes, Tenericutes, Chlamydiae and Fusobacteria. c) Expression of the MfnrdFIE operon induced by addition of 0.1% v/v L-arabinose (green) rescued the JEM164 double knockout (Δ nrdAB , Δ nrdEF ) strain while when gene expression was suppressed with 0.1% v/v D-glucose (brown) the strain failed to recover, as did the strain lacking the vector (red). Growth curves (average of 3 experiments with SD) are shown. d) Mf NrdI activates Mf R2 in an O 2 dependent reaction. HPLC based in vitro RNR activity assays show no activity for R2 protein expressed separately in E. coli (red), while aerobic co-expression with MfnrdI and MfnrdE (green) or MfnrdI (orange) produced an active R2 protein. Anaerobic co-expression (yellow) or incubation of the active R2 with hydroxyurea (light blue) abolishes the activity. Partial activity could be regenerated by the addition of Mf NrdI and redox cycling with dithionite and oxygen, blue and maroon for one and two reduction-oxidation cycles respectively. Data points are shown for triplicate experiments.

Techniques Used: Sequencing, Binding Assay, Variant Assay, Expressing, Double Knockout, Plasmid Preparation, High Performance Liquid Chromatography, In Vitro, Activity Assay, Produced, Incubation

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Article Title: The Scientific Basis and Advantage of Human Experiential Assessment in the quality control of Chinese Herbal Medicines exampling as Schisandrae Chinensis Fructus
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Injection:

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Software:

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    Agilent technologies vtr hplc
    <t>HPLC</t> chromatograms of <t>VtR</t> and chemical structure of (+)-vitisin A ( 1 ), (−)-vitisin B ( 2 ), and ampelopsin C ( 3 ). Retention times for internal standard (4-hydroxy-3-methoxycinnamaldehyde, IS), ampelopsin C ( 3 ), (+)-vitisin A ( 1 ), and (−)-vitisin B ( 2 ) were 15.0, 21.1, 25.7, and 29.7 min, respectively. The traced component of peak 4 with a retention time of 17.9 min was resveratrol.
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    Agilent technologies agilent 1200 hplc
    Performance comparison between EO-pumped <t>HPLC</t> and <t>Agilent</t> 1200 HPLC. (a) HPLC setup with high-pressure on-chip EO pump. L1 and L2: two loops on 10-port valve; E(i): eluent i; V: 10 nL injection valve; C: Waters Atlantis C18 column (75 μm i.d. and 100 mm length); D: Linear UVIS 200 absorbance detector (210 nm); and W: waste. Inset: the other position of the 10-port valve. 6 kV was applied across all pump channels. (b) Typical chromatograms for trypsin digests of BSA, TF, and human IgG from the EO-pumped HPLC. The eluent contained a constant 0.1% trifluoroacetic acid and varying amount of acetonitrile in water. The acetonitrile concentration was initially increased by 3% every 3 min until 56%, then increased by 2% every 2 min until 60%, and then remained at 60% until the completion of the run (see the gradient profile in the top panel). The elution pressure was about 70–80 bar, and the pump rate was ∼160 nL/min. (c) Results from Agilent 1200 HPLC. A flow splitter was used between the 10 nL injection valve and Agilent 1200 pump. The flow rate of the Agilent pump was set at 90 μL/min, resulting in an elution rate of ∼160 nL/min. The gradient stayed at 5% for the first 2 min, then went from 5% to 20% in 15 min, 20% to 40% in the next 30 min, 40% to 60% in the next 40 min, and stayed at 60% to complete the separation. All other conditions were the same as in (b).
    Agilent 1200 Hplc, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies 1100 capillary hplc ion trap ms system
    <t>HPLC-ESI</t> + -MS/MS analysis of oxazolone in enzymatic hydrolysates of rat liver DNA (300 μg) following off-line HPLC purification ( Scheme 2 ). An Agilent <t>1100</t> series capillary HPLC was interfaced to a Thermo-Finnigan TSQ Quantum Ultra mass spectrometer. A Thermo Hypersil-Keystone Hypercarb column (0.5 × 100 mm, 5 μm) was eluted at a flow rate of 12 μl/min with a gradient of isopropanol/acetonitrile (3:1) (solvent B) in 0.05% acetic acid (solvent A). The spray voltage was set to 3.1 kV, the source temperature was 250°C, and the sheath gas pressure was 30 psi. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 247.1→87.1 [M + 2H − dR − CO 2 ] + , and m/z 251.1→91.1 for oxazolone and 15 N 4 -oxazolone, respectively.
    1100 Capillary Hplc Ion Trap Ms System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies afs high performance liquid chromatography hplc analysis
    <t>HPLC-ELSD</t> chromatograms of standard saponins ( A ) and <t>AFS</t> ( B ). ( a ) Anhuienoside E. ( b ) Glycoside St-I4a. ( c ) 3- O - α - l -Rhamnopyranosyl (1 → 2)- β - d -glucopyranosyl oleanolic acid 28- O - β - d -glucopyranosyl (1 → 6)- β - d -glucopyranosyl ester. ( d ) Hemsgiganoside B. ( e ) Flaccidoside II. ( f ) Hederasaponin B.
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    Image Search Results


    HPLC chromatograms of VtR and chemical structure of (+)-vitisin A ( 1 ), (−)-vitisin B ( 2 ), and ampelopsin C ( 3 ). Retention times for internal standard (4-hydroxy-3-methoxycinnamaldehyde, IS), ampelopsin C ( 3 ), (+)-vitisin A ( 1 ), and (−)-vitisin B ( 2 ) were 15.0, 21.1, 25.7, and 29.7 min, respectively. The traced component of peak 4 with a retention time of 17.9 min was resveratrol.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: A Special Ingredient (VtR) Containing Oligostilbenes Isolated from Vitis thunbergii Prevents Bone Loss in Ovariectomized Mice: In Vitro and In Vivo Study

    doi: 10.1155/2013/409421

    Figure Lengend Snippet: HPLC chromatograms of VtR and chemical structure of (+)-vitisin A ( 1 ), (−)-vitisin B ( 2 ), and ampelopsin C ( 3 ). Retention times for internal standard (4-hydroxy-3-methoxycinnamaldehyde, IS), ampelopsin C ( 3 ), (+)-vitisin A ( 1 ), and (−)-vitisin B ( 2 ) were 15.0, 21.1, 25.7, and 29.7 min, respectively. The traced component of peak 4 with a retention time of 17.9 min was resveratrol.

    Article Snippet: HPLC Analysis of VtR HPLC was conducted on an Agilent 1100 series equipped with a G1311A QuatPump, G1379A degasser, G1315B photodiode array detector, and a 1200 series G1329A ALS.

    Techniques: High Performance Liquid Chromatography

    Performance comparison between EO-pumped HPLC and Agilent 1200 HPLC. (a) HPLC setup with high-pressure on-chip EO pump. L1 and L2: two loops on 10-port valve; E(i): eluent i; V: 10 nL injection valve; C: Waters Atlantis C18 column (75 μm i.d. and 100 mm length); D: Linear UVIS 200 absorbance detector (210 nm); and W: waste. Inset: the other position of the 10-port valve. 6 kV was applied across all pump channels. (b) Typical chromatograms for trypsin digests of BSA, TF, and human IgG from the EO-pumped HPLC. The eluent contained a constant 0.1% trifluoroacetic acid and varying amount of acetonitrile in water. The acetonitrile concentration was initially increased by 3% every 3 min until 56%, then increased by 2% every 2 min until 60%, and then remained at 60% until the completion of the run (see the gradient profile in the top panel). The elution pressure was about 70–80 bar, and the pump rate was ∼160 nL/min. (c) Results from Agilent 1200 HPLC. A flow splitter was used between the 10 nL injection valve and Agilent 1200 pump. The flow rate of the Agilent pump was set at 90 μL/min, resulting in an elution rate of ∼160 nL/min. The gradient stayed at 5% for the first 2 min, then went from 5% to 20% in 15 min, 20% to 40% in the next 30 min, 40% to 60% in the next 40 min, and stayed at 60% to complete the separation. All other conditions were the same as in (b).

    Journal: Analytical Chemistry

    Article Title: High-Pressure Open-Channel On-Chip Electroosmotic Pump for Nanoflow High Performance Liquid Chromatography

    doi: 10.1021/ac4040345

    Figure Lengend Snippet: Performance comparison between EO-pumped HPLC and Agilent 1200 HPLC. (a) HPLC setup with high-pressure on-chip EO pump. L1 and L2: two loops on 10-port valve; E(i): eluent i; V: 10 nL injection valve; C: Waters Atlantis C18 column (75 μm i.d. and 100 mm length); D: Linear UVIS 200 absorbance detector (210 nm); and W: waste. Inset: the other position of the 10-port valve. 6 kV was applied across all pump channels. (b) Typical chromatograms for trypsin digests of BSA, TF, and human IgG from the EO-pumped HPLC. The eluent contained a constant 0.1% trifluoroacetic acid and varying amount of acetonitrile in water. The acetonitrile concentration was initially increased by 3% every 3 min until 56%, then increased by 2% every 2 min until 60%, and then remained at 60% until the completion of the run (see the gradient profile in the top panel). The elution pressure was about 70–80 bar, and the pump rate was ∼160 nL/min. (c) Results from Agilent 1200 HPLC. A flow splitter was used between the 10 nL injection valve and Agilent 1200 pump. The flow rate of the Agilent pump was set at 90 μL/min, resulting in an elution rate of ∼160 nL/min. The gradient stayed at 5% for the first 2 min, then went from 5% to 20% in 15 min, 20% to 40% in the next 30 min, 40% to 60% in the next 40 min, and stayed at 60% to complete the separation. All other conditions were the same as in (b).

    Article Snippet: Figure b,c presents a performance comparison between chromatograms from the EO-pumped HPLC (Figure b) and those from an Agilent 1200 HPLC (Figure c).

    Techniques: High Performance Liquid Chromatography, Chromatin Immunoprecipitation, Injection, Concentration Assay, Flow Cytometry

    HPLC-ESI + -MS/MS analysis of oxazolone in enzymatic hydrolysates of rat liver DNA (300 μg) following off-line HPLC purification ( Scheme 2 ). An Agilent 1100 series capillary HPLC was interfaced to a Thermo-Finnigan TSQ Quantum Ultra mass spectrometer. A Thermo Hypersil-Keystone Hypercarb column (0.5 × 100 mm, 5 μm) was eluted at a flow rate of 12 μl/min with a gradient of isopropanol/acetonitrile (3:1) (solvent B) in 0.05% acetic acid (solvent A). The spray voltage was set to 3.1 kV, the source temperature was 250°C, and the sheath gas pressure was 30 psi. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 247.1→87.1 [M + 2H − dR − CO 2 ] + , and m/z 251.1→91.1 for oxazolone and 15 N 4 -oxazolone, respectively.

    Journal: Nucleic Acids Research

    Article Title: Quantitative analysis of the oxidative DNA lesion, 2,2-diamino-4-(2-deoxy-?-d-erythro-pentofuranosyl)amino]-5(2H)-oxazolone (oxazolone), in vitro and in vivo by isotope dilution-capillary HPLC-ESI-MS/MS

    doi: 10.1093/nar/gkl596

    Figure Lengend Snippet: HPLC-ESI + -MS/MS analysis of oxazolone in enzymatic hydrolysates of rat liver DNA (300 μg) following off-line HPLC purification ( Scheme 2 ). An Agilent 1100 series capillary HPLC was interfaced to a Thermo-Finnigan TSQ Quantum Ultra mass spectrometer. A Thermo Hypersil-Keystone Hypercarb column (0.5 × 100 mm, 5 μm) was eluted at a flow rate of 12 μl/min with a gradient of isopropanol/acetonitrile (3:1) (solvent B) in 0.05% acetic acid (solvent A). The spray voltage was set to 3.1 kV, the source temperature was 250°C, and the sheath gas pressure was 30 psi. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 247.1→87.1 [M + 2H − dR − CO 2 ] + , and m/z 251.1→91.1 for oxazolone and 15 N 4 -oxazolone, respectively.

    Article Snippet: Our HPLC-ESI-MS/MS method for 8-oxo-dG employed selected reaction monitoring of the transitions 284.2 [M + H]+ →168.2 [M + 2H − dR]+ (8-oxo-dG) and 289.2 [M + H]+ →173.2 [M + 2H − dR]+ (15 N5 –8-oxo-dG) on an Agilent 1100 capillary HPLC- Ion Trap MS system.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Purification, Flow Cytometry

    HPLC-ESI + -MS/MS analysis of 8-oxo-dG in an enzymatic hydrolysate of rat liver DNA (80 μg). An Agilent 1100 series capillary HPLC-ion trap MS system was used. A Zorbax SB C18 column (0.5 × 150 mm, 5 (m) was maintained at 10°C and eluted at a flow rate of 12 (l/min with a gradient of methanol (solvent B) in 15 mM ammonium acetate (solvent A). The mass spectrometer was operated in the positive ion MS/MS mode. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 284.1→168.0 (M + 2H − dR) + for 8-oxo-dG and the corresponding transition m/z 289.1→173 for [ 15 N 5 ]-8-oxo-dG.

    Journal: Nucleic Acids Research

    Article Title: Quantitative analysis of the oxidative DNA lesion, 2,2-diamino-4-(2-deoxy-?-d-erythro-pentofuranosyl)amino]-5(2H)-oxazolone (oxazolone), in vitro and in vivo by isotope dilution-capillary HPLC-ESI-MS/MS

    doi: 10.1093/nar/gkl596

    Figure Lengend Snippet: HPLC-ESI + -MS/MS analysis of 8-oxo-dG in an enzymatic hydrolysate of rat liver DNA (80 μg). An Agilent 1100 series capillary HPLC-ion trap MS system was used. A Zorbax SB C18 column (0.5 × 150 mm, 5 (m) was maintained at 10°C and eluted at a flow rate of 12 (l/min with a gradient of methanol (solvent B) in 15 mM ammonium acetate (solvent A). The mass spectrometer was operated in the positive ion MS/MS mode. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 284.1→168.0 (M + 2H − dR) + for 8-oxo-dG and the corresponding transition m/z 289.1→173 for [ 15 N 5 ]-8-oxo-dG.

    Article Snippet: Our HPLC-ESI-MS/MS method for 8-oxo-dG employed selected reaction monitoring of the transitions 284.2 [M + H]+ →168.2 [M + 2H − dR]+ (8-oxo-dG) and 289.2 [M + H]+ →173.2 [M + 2H − dR]+ (15 N5 –8-oxo-dG) on an Agilent 1100 capillary HPLC- Ion Trap MS system.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Flow Cytometry

    HPLC-ELSD chromatograms of standard saponins ( A ) and AFS ( B ). ( a ) Anhuienoside E. ( b ) Glycoside St-I4a. ( c ) 3- O - α - l -Rhamnopyranosyl (1 → 2)- β - d -glucopyranosyl oleanolic acid 28- O - β - d -glucopyranosyl (1 → 6)- β - d -glucopyranosyl ester. ( d ) Hemsgiganoside B. ( e ) Flaccidoside II. ( f ) Hederasaponin B.

    Journal: Chinese Medicine

    Article Title: Crude triterpenoid saponins from Anemone flaccida (Di Wu) exert anti-arthritic effects on type II collagen-induced arthritis in rats

    doi: 10.1186/s13020-015-0052-y

    Figure Lengend Snippet: HPLC-ELSD chromatograms of standard saponins ( A ) and AFS ( B ). ( a ) Anhuienoside E. ( b ) Glycoside St-I4a. ( c ) 3- O - α - l -Rhamnopyranosyl (1 → 2)- β - d -glucopyranosyl oleanolic acid 28- O - β - d -glucopyranosyl (1 → 6)- β - d -glucopyranosyl ester. ( d ) Hemsgiganoside B. ( e ) Flaccidoside II. ( f ) Hederasaponin B.

    Article Snippet: HPLC analysis of AFS High performance liquid chromatography (HPLC) analysis was performed using an Agilent 1260 system (Agilent Corp., USA) equipped with a quaternary pump, an auto plate-sampler, a thermostatically controlled column apartment, and an evaporative light scattering detector (ELSD; Alltech Corp., USA).

    Techniques: High Performance Liquid Chromatography