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Phenomenex hplc separation
In vivo production of (2-ethyl-pyridin-4-yl)methanol ( 5 ) from <t>ETA</t> by whole cells of MTb. A culture of MTb strain H37Rv at OD 650 of 1.0 was concentrated 10-fold in 7H9 media, and [1- 14 C]ETA at 0.01 μg/ml was added. ( A ) After incubation at 37°C metabolism of [1- 14 C]ETA was visualized by TLC and autoradiography. Lanes a–h correspond to sequential filtered culture samples taken at 0.2, 0.25, 0.75, 1.5, 2.5, 5.0, 8.5, and 25 h, respectively. Lane i represents media autooxidation after 25 h of incubation without bacterial cells. The metabolites observed cochromatographed with commercial and characterized synthetic samples of ETA ( 1 ), ETA S -oxide ( 2 ), ETA nitrile ( 3 ), and ETA amide ( 4 ). ( B ) Mycobacteria from the same sequential culture aliquots (500 μl) were collected by filtration onto 0.22-μm filter disks under vacuum, they were washed twice with PBS (500 μl), and the cell-associated radioactivity was measured. ( C ) The reversed-phase <t>HPLC</t> retention time of the unknown major metabolite ( 5 ) was used to guide cold large-scale ETA feeding experiments where we isolated unlabeled metabolite that gave a mass of 137 (137.9 MH + ). We assigned this as (2-ethyl-pyridin-4-yl)methanol and confirmed the identity of ( 5 ) by cochromatography with a synthetic characterized alcohol standard. The upper HPLC continuous radiodetector spectrum corresponds to A lane i, media control and the lower spectrum; lane d, time point 1.5 h, where the UV 254 trace of (2-ethyl-pyridin-4-yl)methanol is superimposed in gray.
Hplc Separation, supplied by Phenomenex, used in various techniques. Bioz Stars score: 94/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hplc separation/product/Phenomenex
Average 94 stars, based on 148 article reviews
Price from $9.99 to $1999.99
hplc separation - by Bioz Stars, 2020-08
94/100 stars

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1) Product Images from "Ethionamide activation and sensitivity in multidrug-resistant Mycobacterium tuberculosis"

Article Title: Ethionamide activation and sensitivity in multidrug-resistant Mycobacterium tuberculosis

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi:

In vivo production of (2-ethyl-pyridin-4-yl)methanol ( 5 ) from ETA by whole cells of MTb. A culture of MTb strain H37Rv at OD 650 of 1.0 was concentrated 10-fold in 7H9 media, and [1- 14 C]ETA at 0.01 μg/ml was added. ( A ) After incubation at 37°C metabolism of [1- 14 C]ETA was visualized by TLC and autoradiography. Lanes a–h correspond to sequential filtered culture samples taken at 0.2, 0.25, 0.75, 1.5, 2.5, 5.0, 8.5, and 25 h, respectively. Lane i represents media autooxidation after 25 h of incubation without bacterial cells. The metabolites observed cochromatographed with commercial and characterized synthetic samples of ETA ( 1 ), ETA S -oxide ( 2 ), ETA nitrile ( 3 ), and ETA amide ( 4 ). ( B ) Mycobacteria from the same sequential culture aliquots (500 μl) were collected by filtration onto 0.22-μm filter disks under vacuum, they were washed twice with PBS (500 μl), and the cell-associated radioactivity was measured. ( C ) The reversed-phase HPLC retention time of the unknown major metabolite ( 5 ) was used to guide cold large-scale ETA feeding experiments where we isolated unlabeled metabolite that gave a mass of 137 (137.9 MH + ). We assigned this as (2-ethyl-pyridin-4-yl)methanol and confirmed the identity of ( 5 ) by cochromatography with a synthetic characterized alcohol standard. The upper HPLC continuous radiodetector spectrum corresponds to A lane i, media control and the lower spectrum; lane d, time point 1.5 h, where the UV 254 trace of (2-ethyl-pyridin-4-yl)methanol is superimposed in gray.
Figure Legend Snippet: In vivo production of (2-ethyl-pyridin-4-yl)methanol ( 5 ) from ETA by whole cells of MTb. A culture of MTb strain H37Rv at OD 650 of 1.0 was concentrated 10-fold in 7H9 media, and [1- 14 C]ETA at 0.01 μg/ml was added. ( A ) After incubation at 37°C metabolism of [1- 14 C]ETA was visualized by TLC and autoradiography. Lanes a–h correspond to sequential filtered culture samples taken at 0.2, 0.25, 0.75, 1.5, 2.5, 5.0, 8.5, and 25 h, respectively. Lane i represents media autooxidation after 25 h of incubation without bacterial cells. The metabolites observed cochromatographed with commercial and characterized synthetic samples of ETA ( 1 ), ETA S -oxide ( 2 ), ETA nitrile ( 3 ), and ETA amide ( 4 ). ( B ) Mycobacteria from the same sequential culture aliquots (500 μl) were collected by filtration onto 0.22-μm filter disks under vacuum, they were washed twice with PBS (500 μl), and the cell-associated radioactivity was measured. ( C ) The reversed-phase HPLC retention time of the unknown major metabolite ( 5 ) was used to guide cold large-scale ETA feeding experiments where we isolated unlabeled metabolite that gave a mass of 137 (137.9 MH + ). We assigned this as (2-ethyl-pyridin-4-yl)methanol and confirmed the identity of ( 5 ) by cochromatography with a synthetic characterized alcohol standard. The upper HPLC continuous radiodetector spectrum corresponds to A lane i, media control and the lower spectrum; lane d, time point 1.5 h, where the UV 254 trace of (2-ethyl-pyridin-4-yl)methanol is superimposed in gray.

Techniques Used: In Vivo, Incubation, Thin Layer Chromatography, Autoradiography, Filtration, Radioactivity, High Performance Liquid Chromatography, Isolation

2) Product Images from "Analysis of quaternary ammonium compounds in estuarine sediments by LC-ToF-MS: very high positive mass defects of alkylamine ions provide powerful diagnostic tools for identification and structural elucidation"

Article Title: Analysis of quaternary ammonium compounds in estuarine sediments by LC-ToF-MS: very high positive mass defects of alkylamine ions provide powerful diagnostic tools for identification and structural elucidation

Journal: Analytical chemistry

doi: 10.1021/ac900900y

Reconstructed ion chromatograms of targeted QACs obtained from sewage impacted estuarine sediment (BB). HPLC method 2 was employed. Note that only 10 μL out of a 300 mL extract was injected, illustrating the high sensitivities that can be achieved
Figure Legend Snippet: Reconstructed ion chromatograms of targeted QACs obtained from sewage impacted estuarine sediment (BB). HPLC method 2 was employed. Note that only 10 μL out of a 300 mL extract was injected, illustrating the high sensitivities that can be achieved

Techniques Used: High Performance Liquid Chromatography, Injection

3) Product Images from "Inhibition of Bupropion Metabolism by Selegiline: Mechanism-Based Inactivation of Human CYP2B6 and Characterization of Glutathione and Peptide Adducts"

Article Title: Inhibition of Bupropion Metabolism by Selegiline: Mechanism-Based Inactivation of Human CYP2B6 and Characterization of Glutathione and Peptide Adducts

Journal: Drug Metabolism and Disposition

doi: 10.1124/dmd.112.046979

Effect of selegiline on the reduced CO spectrum and the heme remaining as measured by HPLC. A, portions of the control and selegiline inactivated samples were bubbled with CO in the presence of dithionite, and the reduced CO spectra were monitored between
Figure Legend Snippet: Effect of selegiline on the reduced CO spectrum and the heme remaining as measured by HPLC. A, portions of the control and selegiline inactivated samples were bubbled with CO in the presence of dithionite, and the reduced CO spectra were monitored between

Techniques Used: High Performance Liquid Chromatography

4) Product Images from "Synthesis and Evaluation of Chirally Defined Side Chain Variants of 7-Chloro-4-Aminoquinoline To Overcome Drug Resistance in Malaria Chemotherapy"

Article Title: Synthesis and Evaluation of Chirally Defined Side Chain Variants of 7-Chloro-4-Aminoquinoline To Overcome Drug Resistance in Malaria Chemotherapy

Journal: Antimicrobial Agents and Chemotherapy

doi: 10.1128/AAC.01152-16

HPLC spectra of the enantiomers 7g and 7h and the racemic compound 7i.
Figure Legend Snippet: HPLC spectra of the enantiomers 7g and 7h and the racemic compound 7i.

Techniques Used: High Performance Liquid Chromatography

Related Articles

High Performance Liquid Chromatography:

Article Title: Analysis of quaternary ammonium compounds in estuarine sediments by LC-ToF-MS: very high positive mass defects of alkylamine ions provide powerful diagnostic tools for identification and structural elucidation
Article Snippet: .. HPLC separation of QACs was initially modeled after the method reported by Martinez-Carballo et al., utilizing a Luna C18 column (Phenomenex; 150×2.00 mm, 5 μm). .. The first (Method 1) most closely resembled protocols reported and was utilized to better retain and provide good chromatographic separation of more soluble BAC and DADMAC homologs.

Article Title: Ethionamide activation and sensitivity in multidrug-resistant Mycobacterium tuberculosis
Article Snippet: .. HPLC separation of the [14 C]ETA metabolite mixture was achieved by using a reverse-phase LUNA column [5 μm, C18(2), 250 × 4.6 mm, Phenomenex, Torrence, CA] with a gradient of (0–5 min) 0% acetonitrile, 100% water; then (5–65 min) to 70% acetonitrile; then (65–80 min) to 100% acetonitrile (all solvents contained 0.1% trifluoroacetic acid). .. The retention time of the unknown radiolabeled major metabolite ( 5 ) using continuous radiodetection (β-RAM, INUS Systems, Tampa, FL) was used to guide cold large-scale ETA feeding experiments with up to 1 liter of log-phase MTb H37Rv, to which we fed 10 μg/ml ETA (Sigma-Aldrich).

Article Title: Inhibition of Bupropion Metabolism by Selegiline: Mechanism-Based Inactivation of Human CYP2B6 and Characterization of Glutathione and Peptide Adducts
Article Snippet: .. HPLC separation of the GSH adducts of selegiline was carried out using a Phenomenex Luna C18 reverse phase column (4.6 × 100 mm; Phenomenex). ..

Article Title: Population Pharmacokinetic Study of Prophylactic Fluconazole in Preterm Infants for Prevention of Invasive Candidiasis
Article Snippet: .. Quantification was conducted using an electrospray ionization (ESI) source in the positive mode and the following conditions: curtain gas of 20, gas 1 (nebulizer gas) of 50, gas 2 (heater gas) of 50, charged aerosol detection gas on high, TurboIonSpray (IS) voltage of 5,500 V, entrance potential (EP) of 10 V, collision energy (CE) of 25 V, probe temperature of 500°C, and scan time of 250 ms. HPLC separation was performed using an isocratic mobile phase composed of 10 mM ammonium formate in distilled water (0.1% formic acid) and 0.1% formic acid in acetonitrile (65:35, vol/vol) on a Luna guard column (2 μm, 4.0 by 2.0 mm; Phenomenex, Torrance, CA, USA). ..

Article Title: Determination of Phenolic Acids and Flavonoids in Taraxacum formosanum Kitam by Liquid Chromatography-Tandem Mass Spectrometry Coupled with a Post-Column Derivatization Technique
Article Snippet: .. HPLC Separation Two HPLC columns, namely Vydac 201TP54 C18 and Phenomenex Gemini C18, were compared for separation efficiency of phenolic acids and flavonoids from T. formosanum . .. The conditions for HPLC separation was based on a previous study by Kao et al .

Article Title: Characterization and Quantification of the Compounds of the Ethanolic Extract from Caesalpinia ferrea Stem Bark and Evaluation of Their Mutagenic Activity
Article Snippet: .. HPLC separation was conducted on a Phenomenex (Torrance, CA, USA) Synergi Hydro RP18 (250 × 4.6 mm i.d. ..

Article Title: Synthesis and Evaluation of Chirally Defined Side Chain Variants of 7-Chloro-4-Aminoquinoline To Overcome Drug Resistance in Malaria Chemotherapy
Article Snippet: .. HPLC separation of compound 7g and its enantiomer, 7h, was achieved on a Lux 5μ cellulose-1 (250 mm × 4.60 mm × 5 μm) chiral column (Phenomenex) at 25 ± 3°C, utilizing a mobile phase consisting of a mixture of hexane, isopropanol, and methanol (95:4.5:0.5) with the addition of triethylamine (TEA) (0.8%), at a flow rate of 2.0 ml/min and with a detection wavelength of 254 nm. .. The HPLC profiles of the enantiomers vis-à-vis the racemate clearly suggest that compounds 7g and 7h are chirally pure and that there is no cross contamination of the enantiomers ( ).

Flow Cytometry:

Article Title: Synthesis and Evaluation of Chirally Defined Side Chain Variants of 7-Chloro-4-Aminoquinoline To Overcome Drug Resistance in Malaria Chemotherapy
Article Snippet: .. HPLC separation of compound 7g and its enantiomer, 7h, was achieved on a Lux 5μ cellulose-1 (250 mm × 4.60 mm × 5 μm) chiral column (Phenomenex) at 25 ± 3°C, utilizing a mobile phase consisting of a mixture of hexane, isopropanol, and methanol (95:4.5:0.5) with the addition of triethylamine (TEA) (0.8%), at a flow rate of 2.0 ml/min and with a detection wavelength of 254 nm. .. The HPLC profiles of the enantiomers vis-à-vis the racemate clearly suggest that compounds 7g and 7h are chirally pure and that there is no cross contamination of the enantiomers ( ).

Mass Spectrometry:

Article Title: Population Pharmacokinetic Study of Prophylactic Fluconazole in Preterm Infants for Prevention of Invasive Candidiasis
Article Snippet: .. Quantification was conducted using an electrospray ionization (ESI) source in the positive mode and the following conditions: curtain gas of 20, gas 1 (nebulizer gas) of 50, gas 2 (heater gas) of 50, charged aerosol detection gas on high, TurboIonSpray (IS) voltage of 5,500 V, entrance potential (EP) of 10 V, collision energy (CE) of 25 V, probe temperature of 500°C, and scan time of 250 ms. HPLC separation was performed using an isocratic mobile phase composed of 10 mM ammonium formate in distilled water (0.1% formic acid) and 0.1% formic acid in acetonitrile (65:35, vol/vol) on a Luna guard column (2 μm, 4.0 by 2.0 mm; Phenomenex, Torrance, CA, USA). ..

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    Phenomenex hplc separation
    In vivo production of (2-ethyl-pyridin-4-yl)methanol ( 5 ) from <t>ETA</t> by whole cells of MTb. A culture of MTb strain H37Rv at OD 650 of 1.0 was concentrated 10-fold in 7H9 media, and [1- 14 C]ETA at 0.01 μg/ml was added. ( A ) After incubation at 37°C metabolism of [1- 14 C]ETA was visualized by TLC and autoradiography. Lanes a–h correspond to sequential filtered culture samples taken at 0.2, 0.25, 0.75, 1.5, 2.5, 5.0, 8.5, and 25 h, respectively. Lane i represents media autooxidation after 25 h of incubation without bacterial cells. The metabolites observed cochromatographed with commercial and characterized synthetic samples of ETA ( 1 ), ETA S -oxide ( 2 ), ETA nitrile ( 3 ), and ETA amide ( 4 ). ( B ) Mycobacteria from the same sequential culture aliquots (500 μl) were collected by filtration onto 0.22-μm filter disks under vacuum, they were washed twice with PBS (500 μl), and the cell-associated radioactivity was measured. ( C ) The reversed-phase <t>HPLC</t> retention time of the unknown major metabolite ( 5 ) was used to guide cold large-scale ETA feeding experiments where we isolated unlabeled metabolite that gave a mass of 137 (137.9 MH + ). We assigned this as (2-ethyl-pyridin-4-yl)methanol and confirmed the identity of ( 5 ) by cochromatography with a synthetic characterized alcohol standard. The upper HPLC continuous radiodetector spectrum corresponds to A lane i, media control and the lower spectrum; lane d, time point 1.5 h, where the UV 254 trace of (2-ethyl-pyridin-4-yl)methanol is superimposed in gray.
    Hplc Separation, supplied by Phenomenex, used in various techniques. Bioz Stars score: 94/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc separation/product/Phenomenex
    Average 94 stars, based on 149 article reviews
    Price from $9.99 to $1999.99
    hplc separation - by Bioz Stars, 2020-08
    94/100 stars
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    In vivo production of (2-ethyl-pyridin-4-yl)methanol ( 5 ) from ETA by whole cells of MTb. A culture of MTb strain H37Rv at OD 650 of 1.0 was concentrated 10-fold in 7H9 media, and [1- 14 C]ETA at 0.01 μg/ml was added. ( A ) After incubation at 37°C metabolism of [1- 14 C]ETA was visualized by TLC and autoradiography. Lanes a–h correspond to sequential filtered culture samples taken at 0.2, 0.25, 0.75, 1.5, 2.5, 5.0, 8.5, and 25 h, respectively. Lane i represents media autooxidation after 25 h of incubation without bacterial cells. The metabolites observed cochromatographed with commercial and characterized synthetic samples of ETA ( 1 ), ETA S -oxide ( 2 ), ETA nitrile ( 3 ), and ETA amide ( 4 ). ( B ) Mycobacteria from the same sequential culture aliquots (500 μl) were collected by filtration onto 0.22-μm filter disks under vacuum, they were washed twice with PBS (500 μl), and the cell-associated radioactivity was measured. ( C ) The reversed-phase HPLC retention time of the unknown major metabolite ( 5 ) was used to guide cold large-scale ETA feeding experiments where we isolated unlabeled metabolite that gave a mass of 137 (137.9 MH + ). We assigned this as (2-ethyl-pyridin-4-yl)methanol and confirmed the identity of ( 5 ) by cochromatography with a synthetic characterized alcohol standard. The upper HPLC continuous radiodetector spectrum corresponds to A lane i, media control and the lower spectrum; lane d, time point 1.5 h, where the UV 254 trace of (2-ethyl-pyridin-4-yl)methanol is superimposed in gray.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Ethionamide activation and sensitivity in multidrug-resistant Mycobacterium tuberculosis

    doi:

    Figure Lengend Snippet: In vivo production of (2-ethyl-pyridin-4-yl)methanol ( 5 ) from ETA by whole cells of MTb. A culture of MTb strain H37Rv at OD 650 of 1.0 was concentrated 10-fold in 7H9 media, and [1- 14 C]ETA at 0.01 μg/ml was added. ( A ) After incubation at 37°C metabolism of [1- 14 C]ETA was visualized by TLC and autoradiography. Lanes a–h correspond to sequential filtered culture samples taken at 0.2, 0.25, 0.75, 1.5, 2.5, 5.0, 8.5, and 25 h, respectively. Lane i represents media autooxidation after 25 h of incubation without bacterial cells. The metabolites observed cochromatographed with commercial and characterized synthetic samples of ETA ( 1 ), ETA S -oxide ( 2 ), ETA nitrile ( 3 ), and ETA amide ( 4 ). ( B ) Mycobacteria from the same sequential culture aliquots (500 μl) were collected by filtration onto 0.22-μm filter disks under vacuum, they were washed twice with PBS (500 μl), and the cell-associated radioactivity was measured. ( C ) The reversed-phase HPLC retention time of the unknown major metabolite ( 5 ) was used to guide cold large-scale ETA feeding experiments where we isolated unlabeled metabolite that gave a mass of 137 (137.9 MH + ). We assigned this as (2-ethyl-pyridin-4-yl)methanol and confirmed the identity of ( 5 ) by cochromatography with a synthetic characterized alcohol standard. The upper HPLC continuous radiodetector spectrum corresponds to A lane i, media control and the lower spectrum; lane d, time point 1.5 h, where the UV 254 trace of (2-ethyl-pyridin-4-yl)methanol is superimposed in gray.

    Article Snippet: HPLC separation of the [14 C]ETA metabolite mixture was achieved by using a reverse-phase LUNA column [5 μm, C18(2), 250 × 4.6 mm, Phenomenex, Torrence, CA] with a gradient of (0–5 min) 0% acetonitrile, 100% water; then (5–65 min) to 70% acetonitrile; then (65–80 min) to 100% acetonitrile (all solvents contained 0.1% trifluoroacetic acid).

    Techniques: In Vivo, Incubation, Thin Layer Chromatography, Autoradiography, Filtration, Radioactivity, High Performance Liquid Chromatography, Isolation

    HPLC-DAD chromatograms of S-T 1 – ST-4. HPLC stationary phase: Phenomenex Synergi Polar RP column (150 × 4.6 mm, 4 μm); mobile phase: water ( A ) and acetonitrile ( B ) (both with 0.1% acetic acid), gradient: 65% A to 50% A in 10 min, from 50% to 2% in 10 min; flow rate: 1ml/min; temperature: 25 °C; injection volume: 10 μl.

    Journal: Biologics : Targets & Therapy

    Article Title: The dichloromethane fraction of Stemona tuberosa Lour inhibits tumor cell growth and induces apoptosis of human medullary thyroid carcinoma cells

    doi:

    Figure Lengend Snippet: HPLC-DAD chromatograms of S-T 1 – ST-4. HPLC stationary phase: Phenomenex Synergi Polar RP column (150 × 4.6 mm, 4 μm); mobile phase: water ( A ) and acetonitrile ( B ) (both with 0.1% acetic acid), gradient: 65% A to 50% A in 10 min, from 50% to 2% in 10 min; flow rate: 1ml/min; temperature: 25 °C; injection volume: 10 μl.

    Article Snippet: HPLC–DAD-MS conditions Separations were done on a Phenomenex Synergi Polar RP column (150 × 4.6 mm, 4 μm particle size, Phenomenex.

    Techniques: High Performance Liquid Chromatography, Flow Cytometry, Injection

    Reconstructed ion chromatograms of targeted QACs obtained from sewage impacted estuarine sediment (BB). HPLC method 2 was employed. Note that only 10 μL out of a 300 mL extract was injected, illustrating the high sensitivities that can be achieved

    Journal: Analytical chemistry

    Article Title: Analysis of quaternary ammonium compounds in estuarine sediments by LC-ToF-MS: very high positive mass defects of alkylamine ions provide powerful diagnostic tools for identification and structural elucidation

    doi: 10.1021/ac900900y

    Figure Lengend Snippet: Reconstructed ion chromatograms of targeted QACs obtained from sewage impacted estuarine sediment (BB). HPLC method 2 was employed. Note that only 10 μL out of a 300 mL extract was injected, illustrating the high sensitivities that can be achieved

    Article Snippet: HPLC separation of QACs was initially modeled after the method reported by Martinez-Carballo et al., utilizing a Luna C18 column (Phenomenex; 150×2.00 mm, 5 μm).

    Techniques: High Performance Liquid Chromatography, Injection

    Effect of selegiline on the reduced CO spectrum and the heme remaining as measured by HPLC. A, portions of the control and selegiline inactivated samples were bubbled with CO in the presence of dithionite, and the reduced CO spectra were monitored between

    Journal: Drug Metabolism and Disposition

    Article Title: Inhibition of Bupropion Metabolism by Selegiline: Mechanism-Based Inactivation of Human CYP2B6 and Characterization of Glutathione and Peptide Adducts

    doi: 10.1124/dmd.112.046979

    Figure Lengend Snippet: Effect of selegiline on the reduced CO spectrum and the heme remaining as measured by HPLC. A, portions of the control and selegiline inactivated samples were bubbled with CO in the presence of dithionite, and the reduced CO spectra were monitored between

    Article Snippet: HPLC separation of the GSH adducts of selegiline was carried out using a Phenomenex Luna C18 reverse phase column (4.6 × 100 mm; Phenomenex).

    Techniques: High Performance Liquid Chromatography