hplc pump  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hplc pump
    Hplc Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc pump/product/Thermo Fisher
    Average 99 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    hplc pump - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis
    Article Snippet: .. High pH One-D reverse phase chromatography fractionation Fractionation of the peptide mixture was performed on a Dionex Ultimate 3000 HPLC system equipped with an UltiMate 3000 RS pump, an UltiMate 3000 RS column compartment and an UltiMate 3000 RS autosampler (Dionex, Olten/Switzerland). .. The column was initially equilibrated for 70 min in buffer A (20 mM ammonium formate, pH 10) as described above before sample injection.

    Isolation:

    Article Title: The cranberry flavonoids PAC DP-9 and quercetin aglycone induce cytotoxicity and cell cycle arrest and increase cisplatin sensitivity in ovarian cancer cells
    Article Snippet: .. Serial application of Dionex UltiMate® 3000 LC system consisting of UltiMate 3000 RS Pump, UltiMate 3000 RS Autosampler, UltiMate 3000 RS Column Compartment, and UltiMate 3000 RS Diode Array Detector coupled with Applied Biosystems API 3000 triple quad LC-MS/MS mass spectrometer has allowed the reproducible isolation and characterization of individual PACs with up to the 10th degree of polymerization in > 95% purity in our laboratories. .. So far, we have successfully isolated 19 high-purity individual cranberry flavonoids, characterized their structures by LC-APCI-MS, and used them as primary materials to determine bioactivity.

    Fractionation:

    Article Title: iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis
    Article Snippet: .. High pH One-D reverse phase chromatography fractionation Fractionation of the peptide mixture was performed on a Dionex Ultimate 3000 HPLC system equipped with an UltiMate 3000 RS pump, an UltiMate 3000 RS column compartment and an UltiMate 3000 RS autosampler (Dionex, Olten/Switzerland). .. The column was initially equilibrated for 70 min in buffer A (20 mM ammonium formate, pH 10) as described above before sample injection.

    Mass Spectrometry:

    Article Title: Pharmacokinetic Interaction Between Prasugrel and Ritonavir in Healthy Volunteers
    Article Snippet: .. The DBS concentrations of prasugrel AM midazolam, omeprazole, bupropion and flurbiprofen were measured on a LC-MS/MS platform consisting of a 5500 QTtrap® triple quadrupole linear ion trap mass spectrometer equipped with a TurboIon Spray™ interface (AB Sciex, Darmstadt, Germany) and an Ultimate 3000 RS pump (Dionex) as the LC system. .. Data were acquired and processed using Analyst software (version 1.5.1; AB Sciex).

    Article Title: Flavonoids and darkness lower PCD in senescing Vitis vinifera suspension cell cultures
    Article Snippet: .. Multi-stage Mass Spectrometry (MSn ) experiments and liquid chromatography - electrospray ionization - mass spectrometry (LC-ESI-MS) analyses were achieved by a Finnigan LXQ Linear Ion Trap, operating in the negative ion mode, coupled with a Dionex UltiMate 3000 RS Pump and equipped with a Dionex UltiMate RS 3000 Autosampler (Thermo Scientific, San Jose, CA, USA). .. Methanolic standard solutions (200 μg ml−1 ) of gallic acid, quercetin glucoside, quercetin, malvidin-glucoside and kaempferol were infused into the ion source at a flow rate of 10 μl min−1 , with the aid of a syringe pump, in order to obtain the corresponding MSn spectra (see ).

    Article Title: The cranberry flavonoids PAC DP-9 and quercetin aglycone induce cytotoxicity and cell cycle arrest and increase cisplatin sensitivity in ovarian cancer cells
    Article Snippet: .. Serial application of Dionex UltiMate® 3000 LC system consisting of UltiMate 3000 RS Pump, UltiMate 3000 RS Autosampler, UltiMate 3000 RS Column Compartment, and UltiMate 3000 RS Diode Array Detector coupled with Applied Biosystems API 3000 triple quad LC-MS/MS mass spectrometer has allowed the reproducible isolation and characterization of individual PACs with up to the 10th degree of polymerization in > 95% purity in our laboratories. .. So far, we have successfully isolated 19 high-purity individual cranberry flavonoids, characterized their structures by LC-APCI-MS, and used them as primary materials to determine bioactivity.

    Article Title: Systematic metabolic profiling and bioactivity assays for bioconversion of Aceraceae family
    Article Snippet: .. The Thermo Fischer Scientific LTQ XL linear ion trap mass spectrometry system used in the present study consisted of an electrospray interface (Thermo Fischer Scientific, San José, CA, USA) coupled with a DIONEX UltiMate 3000 RS Pump, RS Autosampler, RS Column Compartment, and RS Diode Array Detector (Dionex Corporation, Sunnyvale, CA, USA). .. The samples were separated on a Thermo Scientific Syncronis C18 UHPLC column with 1.7 μm particle size.

    Article Title: Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts
    Article Snippet: .. The UHPLC-LTQ-IT-MS/MS analysis was performed using a Thermo Fisher Scientific LTQ XL linear ion trap mass spectrometer consisting of an electrospray interface (Thermo Fisher Scientific, San José, CA), coupled with a DIONEX UltiMate 3000 RS pump, RS autosampler, RS column compartment, and RS diode array detector (Dionex Corporation, Sunnyvale, USA). .. The sample analytes were separated on a Thermo Scientific Syncronis C18 UHPLC column (100 mm length × 2.1 mm i.d., 1.7-μm particle size).

    Article Title: Comprehensive Secondary Metabolite Profiling Toward Delineating the Solid and Submerged-State Fermentation of Aspergillus oryzae KCCM 12698
    Article Snippet: .. UHPLC-LTQ-IT-MS/MS analysis was performed using the method partially adapted from Oh et al. ( ) on the LTQ-XL ion trap mass spectrometer equipped with an electrospray interface (Thermo Fisher Scientific, San José, CA) coupled with DIONEX UltiMate 3000 RS Pump, RS Autosampler, RS Column Compartment and RS Diode Array Detector (Dionex Corporation, Sunnyvale, USA). .. The samples were separated on a Thermo Scientific Syncronis C18 UHPLC column with a 1.7 μm particle size.

    Article Title: Strategy for Screening of Antioxidant Compounds from Two Ulmaceae Species Based on Liquid Chromatography-Mass Spectrometry
    Article Snippet: .. The analysis was conducted using an LTQ XL ion-trap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) coupled with a Dionex UHPLC system (Dionex Corporation, Sunnyvale, CA, USA) consisting of an UltiMate 3000 RS (Rapid Separation) Pump, an UltiMate 3000 RS Autosampler, and an UltiMate RS 3000 Column Compartment with a Thermostable Column area and an UltiMate RS 3000 Diode Array Detector (Dionex Corp., Sunnyvale, CA, USA). .. Each sample (10 μL) was injected into a Thermo Scientific Syncronis C18 UHPLC column (100 mm length × 2.1 mm inner diameter, × 1.7-μm particle size) at the flow rate of 0.3 mL/min; the column temperature was retained at 35 °C.

    Reversed-phase Chromatography:

    Article Title: iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis
    Article Snippet: .. High pH One-D reverse phase chromatography fractionation Fractionation of the peptide mixture was performed on a Dionex Ultimate 3000 HPLC system equipped with an UltiMate 3000 RS pump, an UltiMate 3000 RS column compartment and an UltiMate 3000 RS autosampler (Dionex, Olten/Switzerland). .. The column was initially equilibrated for 70 min in buffer A (20 mM ammonium formate, pH 10) as described above before sample injection.

    Liquid Chromatography:

    Article Title: Flavonoids and darkness lower PCD in senescing Vitis vinifera suspension cell cultures
    Article Snippet: .. Multi-stage Mass Spectrometry (MSn ) experiments and liquid chromatography - electrospray ionization - mass spectrometry (LC-ESI-MS) analyses were achieved by a Finnigan LXQ Linear Ion Trap, operating in the negative ion mode, coupled with a Dionex UltiMate 3000 RS Pump and equipped with a Dionex UltiMate RS 3000 Autosampler (Thermo Scientific, San Jose, CA, USA). .. Methanolic standard solutions (200 μg ml−1 ) of gallic acid, quercetin glucoside, quercetin, malvidin-glucoside and kaempferol were infused into the ion source at a flow rate of 10 μl min−1 , with the aid of a syringe pump, in order to obtain the corresponding MSn spectra (see ).

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  • 88
    Thermo Fisher surveyor hplc pump
    Genotoxicity of PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of PhIP for 24 h. (A) Genotoxicity was evaluated by <t>HPLC-MS/MS</t> quantification of <t>dG-C8-PhIP</t> in DNA. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; *, p
    Surveyor Hplc Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/surveyor hplc pump/product/Thermo Fisher
    Average 88 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    surveyor hplc pump - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    85
    Thermo Fisher high pressure liquid chromatography pump
    Immunity generated by recombinant LJM11 salivary protein (rLJM11) is polarized toward a T-cell helper type 1 (Th1) response . ( a ) <t>High-pressure</t> <t>liquid</t> <t>chromatography</t> (HPLC) purification of rLJM11 (↓). Inset: silver-stained gel of purified rLJM11. ( b–f ) C57BL/6 mice were immunized with 500 ng of either rLJM11 or BSA in the left ear. ( b ) Total IgG levels against rLJM11 and the IgG2a:IgG1 ratio. IFN-γ, IL-10, and IL-4 production following in vitro stimulation of lymph node (LN, c ) and ( d ) spleen cells with rLJM11. ( e ) Delayed-type hypersensitivity (DTH) response in mice immunized with rLJM11 (▪) or BSA (•). ( f ) The absolute number of CD4 + T cells per ear following sand fly challenge. * P
    High Pressure Liquid Chromatography Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high pressure liquid chromatography pump/product/Thermo Fisher
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    high pressure liquid chromatography pump - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    89
    Thermo Fisher binary hplc pump
    Immunity generated by recombinant LJM11 salivary protein (rLJM11) is polarized toward a T-cell helper type 1 (Th1) response . ( a ) <t>High-pressure</t> <t>liquid</t> <t>chromatography</t> (HPLC) purification of rLJM11 (↓). Inset: silver-stained gel of purified rLJM11. ( b–f ) C57BL/6 mice were immunized with 500 ng of either rLJM11 or BSA in the left ear. ( b ) Total IgG levels against rLJM11 and the IgG2a:IgG1 ratio. IFN-γ, IL-10, and IL-4 production following in vitro stimulation of lymph node (LN, c ) and ( d ) spleen cells with rLJM11. ( e ) Delayed-type hypersensitivity (DTH) response in mice immunized with rLJM11 (▪) or BSA (•). ( f ) The absolute number of CD4 + T cells per ear following sand fly challenge. * P
    Binary Hplc Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/binary hplc pump/product/Thermo Fisher
    Average 89 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    binary hplc pump - by Bioz Stars, 2020-07
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    88
    Thermo Fisher accela quaternary hplc pump
    Immunity generated by recombinant LJM11 salivary protein (rLJM11) is polarized toward a T-cell helper type 1 (Th1) response . ( a ) <t>High-pressure</t> <t>liquid</t> <t>chromatography</t> (HPLC) purification of rLJM11 (↓). Inset: silver-stained gel of purified rLJM11. ( b–f ) C57BL/6 mice were immunized with 500 ng of either rLJM11 or BSA in the left ear. ( b ) Total IgG levels against rLJM11 and the IgG2a:IgG1 ratio. IFN-γ, IL-10, and IL-4 production following in vitro stimulation of lymph node (LN, c ) and ( d ) spleen cells with rLJM11. ( e ) Delayed-type hypersensitivity (DTH) response in mice immunized with rLJM11 (▪) or BSA (•). ( f ) The absolute number of CD4 + T cells per ear following sand fly challenge. * P
    Accela Quaternary Hplc Pump, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accela quaternary hplc pump/product/Thermo Fisher
    Average 88 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    accela quaternary hplc pump - by Bioz Stars, 2020-07
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    Image Search Results


    Genotoxicity of PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of PhIP for 24 h. (A) Genotoxicity was evaluated by HPLC-MS/MS quantification of dG-C8-PhIP in DNA. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; *, p

    Journal: PLoS ONE

    Article Title: Combined Genotoxic Effects of a Polycyclic Aromatic Hydrocarbon (B(a)P) and an Heterocyclic Amine (PhIP) in Relation to Colorectal Carcinogenesis

    doi: 10.1371/journal.pone.0058591

    Figure Lengend Snippet: Genotoxicity of PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of PhIP for 24 h. (A) Genotoxicity was evaluated by HPLC-MS/MS quantification of dG-C8-PhIP in DNA. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; *, p

    Article Snippet: The dG-C8-PhIP adducts were quantified by HPLC-MS/MS using of a Surveyor HPLC pump (Thermo Scientific, Les Ullis, France) associated with a TSQ Quantum Discovery Max triple quadrupole (Thermo Scientific, Les Ullis, France).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    Synergic genotoxicity of B( a )P and PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of BaP and PhIP for 24 h. (A) Genotoxicity was evaluated by quantification by HPLC-MS/MS quantification of B( a )P- and PhIP-derived DNA adducts. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; **, p

    Journal: PLoS ONE

    Article Title: Combined Genotoxic Effects of a Polycyclic Aromatic Hydrocarbon (B(a)P) and an Heterocyclic Amine (PhIP) in Relation to Colorectal Carcinogenesis

    doi: 10.1371/journal.pone.0058591

    Figure Lengend Snippet: Synergic genotoxicity of B( a )P and PhIP in Apc +/+ and Apc Min/+ cell lines. Apc +/+ and Apc Min/+ cells were treated with the indicated concentrations of BaP and PhIP for 24 h. (A) Genotoxicity was evaluated by quantification by HPLC-MS/MS quantification of B( a )P- and PhIP-derived DNA adducts. (B) Genotoxicity was evaluated with γHAX ICW assay. Bars represent the average of at least three independent experiments with SEM. Statistically significant increase in H2AX phosphorylation compared with DMSO control using Student’s test; **, p

    Article Snippet: The dG-C8-PhIP adducts were quantified by HPLC-MS/MS using of a Surveyor HPLC pump (Thermo Scientific, Les Ullis, France) associated with a TSQ Quantum Discovery Max triple quadrupole (Thermo Scientific, Les Ullis, France).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Derivative Assay

    Immunity generated by recombinant LJM11 salivary protein (rLJM11) is polarized toward a T-cell helper type 1 (Th1) response . ( a ) High-pressure liquid chromatography (HPLC) purification of rLJM11 (↓). Inset: silver-stained gel of purified rLJM11. ( b–f ) C57BL/6 mice were immunized with 500 ng of either rLJM11 or BSA in the left ear. ( b ) Total IgG levels against rLJM11 and the IgG2a:IgG1 ratio. IFN-γ, IL-10, and IL-4 production following in vitro stimulation of lymph node (LN, c ) and ( d ) spleen cells with rLJM11. ( e ) Delayed-type hypersensitivity (DTH) response in mice immunized with rLJM11 (▪) or BSA (•). ( f ) The absolute number of CD4 + T cells per ear following sand fly challenge. * P

    Journal: The Journal of Investigative Dermatology

    Article Title: Immunity to Sand Fly Salivary Protein LJM11 Modulates Host Response to Vector-Transmitted Leishmania Conferring Ulcer-Free Protection

    doi: 10.1038/jid.2012.205

    Figure Lengend Snippet: Immunity generated by recombinant LJM11 salivary protein (rLJM11) is polarized toward a T-cell helper type 1 (Th1) response . ( a ) High-pressure liquid chromatography (HPLC) purification of rLJM11 (↓). Inset: silver-stained gel of purified rLJM11. ( b–f ) C57BL/6 mice were immunized with 500 ng of either rLJM11 or BSA in the left ear. ( b ) Total IgG levels against rLJM11 and the IgG2a:IgG1 ratio. IFN-γ, IL-10, and IL-4 production following in vitro stimulation of lymph node (LN, c ) and ( d ) spleen cells with rLJM11. ( e ) Delayed-type hypersensitivity (DTH) response in mice immunized with rLJM11 (▪) or BSA (•). ( f ) The absolute number of CD4 + T cells per ear following sand fly challenge. * P

    Article Snippet: Expressed protein was purified as previously described ( ) and by using a GS2000SW molecular sieving column connected to a Dionex high-pressure liquid chromatography pump (Thermo Fischer Scientific, Waltham, MA).

    Techniques: Generated, Recombinant, High Performance Liquid Chromatography, Purification, Staining, Mouse Assay, In Vitro