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Mutation of the proposed amychelin biosynthetic gene cluster abrogates amychelin production and aerial hyphae inhibition by <t>Amycolatopsis</t> sp. AA4 A. Amychelin biosynthetic gene cluster. Red: NRPS genes, Light Blue: monoxygenase/oxidases, Green: siderophore transport/export, Purple: salicylate synthesis/activation, Gray: mbtH protein, Black: TetR-like regulator. X’s denote genes deleted in respective mutants. B–C. <t>HPLC-MS</t> analysis of minimal medium extracts of mutant strains by UV (A) and MS (B). The ∆ amcG strain makes no detectable amychelin, while the ∆ amcK strain makes only trace amounts. D. The ∆ amcG strain does not inhibit aerial hyphae formation in a nearby colony of S. coelicolor on R2YE(second panel from left). Morphologies of individual colonies of M145, wt AA4, and ∆ amcG are also shown.
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1) Product Images from "Interspecies modulation of bacterial development through iron competition and siderophore piracy"

Article Title: Interspecies modulation of bacterial development through iron competition and siderophore piracy

Journal: Molecular microbiology

doi: 10.1111/mmi.12008

Mutation of the proposed amychelin biosynthetic gene cluster abrogates amychelin production and aerial hyphae inhibition by Amycolatopsis sp. AA4 A. Amychelin biosynthetic gene cluster. Red: NRPS genes, Light Blue: monoxygenase/oxidases, Green: siderophore transport/export, Purple: salicylate synthesis/activation, Gray: mbtH protein, Black: TetR-like regulator. X’s denote genes deleted in respective mutants. B–C. HPLC-MS analysis of minimal medium extracts of mutant strains by UV (A) and MS (B). The ∆ amcG strain makes no detectable amychelin, while the ∆ amcK strain makes only trace amounts. D. The ∆ amcG strain does not inhibit aerial hyphae formation in a nearby colony of S. coelicolor on R2YE(second panel from left). Morphologies of individual colonies of M145, wt AA4, and ∆ amcG are also shown.
Figure Legend Snippet: Mutation of the proposed amychelin biosynthetic gene cluster abrogates amychelin production and aerial hyphae inhibition by Amycolatopsis sp. AA4 A. Amychelin biosynthetic gene cluster. Red: NRPS genes, Light Blue: monoxygenase/oxidases, Green: siderophore transport/export, Purple: salicylate synthesis/activation, Gray: mbtH protein, Black: TetR-like regulator. X’s denote genes deleted in respective mutants. B–C. HPLC-MS analysis of minimal medium extracts of mutant strains by UV (A) and MS (B). The ∆ amcG strain makes no detectable amychelin, while the ∆ amcK strain makes only trace amounts. D. The ∆ amcG strain does not inhibit aerial hyphae formation in a nearby colony of S. coelicolor on R2YE(second panel from left). Morphologies of individual colonies of M145, wt AA4, and ∆ amcG are also shown.

Techniques Used: Mutagenesis, Inhibition, Activation Assay, High Performance Liquid Chromatography, Mass Spectrometry

Iron/siderophore availability influences colony morphology of wild-type and ∆ amcG strains of Amycolatopsis sp. AA4 A. A spectrum of colony morphologies is observed in wild-type and ∆ amcG colonies grown on media with a range of iron concentrations, or the iron chelator 2,2’-dipyridyl, included. Iron supplementation does not restore robust wrinkling (as seen in the wild type grown with 1µM FeCl 3 ) in the ∆ amcG colonies. B. Amychelin production is repressed by iron concentrations > 10 µM. Amycolatopsis sp. AA4 was grown in liquid minimal medium with various concentrations of iron added. The amount of amychelin or ferric-amychelin was quantified by HPLC-MS. C. Amychelin diffusing from the wild type restores the wrinkly colony morphology of the ∆ amcG strain (middle colony). No iron was added to the medium. A ∆ amcG colony at a greater distance is not affected. D. The siderophore deferrioxamine-E (DFO-E) restores the colony morphology of the ∆ amcG strain (left colony). No iron was added to the medium.
Figure Legend Snippet: Iron/siderophore availability influences colony morphology of wild-type and ∆ amcG strains of Amycolatopsis sp. AA4 A. A spectrum of colony morphologies is observed in wild-type and ∆ amcG colonies grown on media with a range of iron concentrations, or the iron chelator 2,2’-dipyridyl, included. Iron supplementation does not restore robust wrinkling (as seen in the wild type grown with 1µM FeCl 3 ) in the ∆ amcG colonies. B. Amychelin production is repressed by iron concentrations > 10 µM. Amycolatopsis sp. AA4 was grown in liquid minimal medium with various concentrations of iron added. The amount of amychelin or ferric-amychelin was quantified by HPLC-MS. C. Amychelin diffusing from the wild type restores the wrinkly colony morphology of the ∆ amcG strain (middle colony). No iron was added to the medium. A ∆ amcG colony at a greater distance is not affected. D. The siderophore deferrioxamine-E (DFO-E) restores the colony morphology of the ∆ amcG strain (left colony). No iron was added to the medium.

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

2) Product Images from "Novel Conjugates of 1,3-Diacylglycerol and Lipoic Acid: Synthesis, DPPH Assay, and RP-LC-MS-APCI Analysis"

Article Title: Novel Conjugates of 1,3-Diacylglycerol and Lipoic Acid: Synthesis, DPPH Assay, and RP-LC-MS-APCI Analysis

Journal: Journal of Lipids

doi: 10.1155/2011/419809

RP-HPLC-MS-APCI total ion and retention time of the test compounds. (a) LA; (b) DHLA; (c) DO; (d) DOLA; (e) DODHLA ( Table 2 is referred for abbreviations).
Figure Legend Snippet: RP-HPLC-MS-APCI total ion and retention time of the test compounds. (a) LA; (b) DHLA; (c) DO; (d) DOLA; (e) DODHLA ( Table 2 is referred for abbreviations).

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

3) Product Images from "Beauveria bassiana: a new N6-(2-hydroxyethyl)-adenosine–producing fungus"

Article Title: Beauveria bassiana: a new N6-(2-hydroxyethyl)-adenosine–producing fungus

Journal: Mycology

doi: 10.1080/21501203.2017.1375040

HPLC chromatograms of HEA standard solution and the water extract from mycelium of strain 351. Samples were eluted in the mobile phase, which consisted of acetonitrile: double-distilled H 2 O (0.1% acetic acid) (5:95), for 20 min at 25°C. The flow rate was 1 mL/min and the injection volume was 10 μL. HEA was monitored and quantified at 260 nm.
Figure Legend Snippet: HPLC chromatograms of HEA standard solution and the water extract from mycelium of strain 351. Samples were eluted in the mobile phase, which consisted of acetonitrile: double-distilled H 2 O (0.1% acetic acid) (5:95), for 20 min at 25°C. The flow rate was 1 mL/min and the injection volume was 10 μL. HEA was monitored and quantified at 260 nm.

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry, Injection

4) Product Images from "Phytochemical Composition, Antioxidant, Antimicrobial and in Vivo Anti-inflammatory Activity of Traditionally Used Romanian Ajuga laxmannii (Murray) Benth. (“Nobleman’s Beard” – Barba Împăratului)"

Article Title: Phytochemical Composition, Antioxidant, Antimicrobial and in Vivo Anti-inflammatory Activity of Traditionally Used Romanian Ajuga laxmannii (Murray) Benth. (“Nobleman’s Beard” – Barba Împăratului)

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2018.00007

HPLC chromatogram of polyphenols from A. laxmannii aerial parts extract. The identified compounds: chlorogenic acid (1), isoquercitrin (2), rutin (3), quercitrin (4), luteolin (5), apigenin (6).
Figure Legend Snippet: HPLC chromatogram of polyphenols from A. laxmannii aerial parts extract. The identified compounds: chlorogenic acid (1), isoquercitrin (2), rutin (3), quercitrin (4), luteolin (5), apigenin (6).

Techniques Used: High Performance Liquid Chromatography

5) Product Images from "Monoamine Oxidase-A Inhibition and Associated Antioxidant Activity in Plant Extracts with Potential Antidepressant Actions"

Article Title: Monoamine Oxidase-A Inhibition and Associated Antioxidant Activity in Plant Extracts with Potential Antidepressant Actions

Journal: BioMed Research International

doi: 10.1155/2018/4810394

HPLC chromatograms of extracts from H. perforatum flowers. (a) Detection of hypericins at 590 nm. 1 : protopseudohypericin; 2 : pseudohypericin; 3 : protohypericin; and 4 : hypericin. (b) Detection of phenols and flavonoids at 265 nm. 1 : chlorogenic acid; 2 : rutin; 3 : hyperoside; 4 : isoquercitrin; 5 : miquelianin; 6 : acetyl hyperoside; 7 : quercitrin; 8 : quercetin; and 9 : biapigenin. (c) Detection of phloroglucinols at 280 nm. 1 : hyperfirin, 2 : adhyperfirin; 3 : hyperforin; and 4 : adhyperforin.
Figure Legend Snippet: HPLC chromatograms of extracts from H. perforatum flowers. (a) Detection of hypericins at 590 nm. 1 : protopseudohypericin; 2 : pseudohypericin; 3 : protohypericin; and 4 : hypericin. (b) Detection of phenols and flavonoids at 265 nm. 1 : chlorogenic acid; 2 : rutin; 3 : hyperoside; 4 : isoquercitrin; 5 : miquelianin; 6 : acetyl hyperoside; 7 : quercitrin; 8 : quercetin; and 9 : biapigenin. (c) Detection of phloroglucinols at 280 nm. 1 : hyperfirin, 2 : adhyperfirin; 3 : hyperforin; and 4 : adhyperforin.

Techniques Used: High Performance Liquid Chromatography

6) Product Images from "Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains – An Unusual Secondary Metabolite with Various Properties"

Article Title: Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains – An Unusual Secondary Metabolite with Various Properties

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2017.00221

Production of polyketomycin and foxicin in Streptomyces diastatochromogenes Tü6028. (A) HPLC chromatogram of S. diastatochromogenes Tü6028 wild type at λ430 nm with polyketomycin structure (top) and of Δ pokOIV mutant at λ320 nm (below); (B) Production of foxicin A (▴) and polyketomycin (O) in S. diastatochromogenes Tü6028 wild type; (C) Morphology of S. diastatochromogenes Tü6028 wild type (left) and Δ pokOIV mutant (right). On the plate, the Δ pokOIV mutant appears yellow and deficient in producing spores and melanins (dark color).
Figure Legend Snippet: Production of polyketomycin and foxicin in Streptomyces diastatochromogenes Tü6028. (A) HPLC chromatogram of S. diastatochromogenes Tü6028 wild type at λ430 nm with polyketomycin structure (top) and of Δ pokOIV mutant at λ320 nm (below); (B) Production of foxicin A (▴) and polyketomycin (O) in S. diastatochromogenes Tü6028 wild type; (C) Morphology of S. diastatochromogenes Tü6028 wild type (left) and Δ pokOIV mutant (right). On the plate, the Δ pokOIV mutant appears yellow and deficient in producing spores and melanins (dark color).

Techniques Used: High Performance Liquid Chromatography, Mutagenesis

7) Product Images from "Identification of a novel hydroxylated metabolite of 2,2′,3,5′,6-pentachlorobiphenyl formed in whole poplar plants"

Article Title: Identification of a novel hydroxylated metabolite of 2,2′,3,5′,6-pentachlorobiphenyl formed in whole poplar plants

Journal: Environmental science and pollution research international

doi: 10.1007/s11356-015-5939-8

Analysis of relative abundance of the two atropisomers of 4′-OH-PCB95 on chiral column by HPLC-MS. The chromatogram was recorded in the SIM mode ( m/z = 341).
Figure Legend Snippet: Analysis of relative abundance of the two atropisomers of 4′-OH-PCB95 on chiral column by HPLC-MS. The chromatogram was recorded in the SIM mode ( m/z = 341).

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

8) Product Images from "Phytochemical Composition, Antioxidant, Antimicrobial and in Vivo Anti-inflammatory Activity of Traditionally Used Romanian Ajuga laxmannii (Murray) Benth. (“Nobleman’s Beard” – Barba Împăratului)"

Article Title: Phytochemical Composition, Antioxidant, Antimicrobial and in Vivo Anti-inflammatory Activity of Traditionally Used Romanian Ajuga laxmannii (Murray) Benth. (“Nobleman’s Beard” – Barba Împăratului)

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2018.00007

HPLC chromatogram of polyphenols from A. laxmannii aerial parts extract. The identified compounds: chlorogenic acid (1), isoquercitrin (2), rutin (3), quercitrin (4), luteolin (5), apigenin (6).
Figure Legend Snippet: HPLC chromatogram of polyphenols from A. laxmannii aerial parts extract. The identified compounds: chlorogenic acid (1), isoquercitrin (2), rutin (3), quercitrin (4), luteolin (5), apigenin (6).

Techniques Used: High Performance Liquid Chromatography

9) Product Images from "Genomic diversity in ochratoxigenic and non ochratoxigenic strains of Aspergillus carbonarius"

Article Title: Genomic diversity in ochratoxigenic and non ochratoxigenic strains of Aspergillus carbonarius

Journal: Scientific Reports

doi: 10.1038/s41598-018-23802-8

HPLC-FLD chromatograms and mass spectra of ( a ) the OTA producing strain A. carbonarius A-1137 (OTA standard retention time: 4.854 min), and ( b ) the non-ochratoxigenic strain of A. carbonarius A-2579 after incubation at 15 °C for 10 days on Czapek Yeast extract Agar.
Figure Legend Snippet: HPLC-FLD chromatograms and mass spectra of ( a ) the OTA producing strain A. carbonarius A-1137 (OTA standard retention time: 4.854 min), and ( b ) the non-ochratoxigenic strain of A. carbonarius A-2579 after incubation at 15 °C for 10 days on Czapek Yeast extract Agar.

Techniques Used: High Performance Liquid Chromatography, Incubation

10) Product Images from "Study on the presence of ochratoxin α in cultures of ochratoxigenic and non- ochratoxigenic strains of Aspergillus carbonarius"

Article Title: Study on the presence of ochratoxin α in cultures of ochratoxigenic and non- ochratoxigenic strains of Aspergillus carbonarius

Journal: PLoS ONE

doi: 10.1371/journal.pone.0185986

Selected extracted ion chromatograms of fungal extracts and (a) OTA and (b) OTα standards analysed using HPLC-MS and an OTA/OTα-producing strain ( A . carbonarius A-1657) and a non-OTA/OTα-producing strain ( A . carbonarius A-2579),
Figure Legend Snippet: Selected extracted ion chromatograms of fungal extracts and (a) OTA and (b) OTα standards analysed using HPLC-MS and an OTA/OTα-producing strain ( A . carbonarius A-1657) and a non-OTA/OTα-producing strain ( A . carbonarius A-2579),

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

Selected chromatograms of fungal extracts analysed using HPLC coupled to a fluorescence detector of (a) an OTA/OTα-producing strain ( A . carbonarius A-2034) (OTα retention time: 2.773 min; OTA retention time: 4.890 min) and (b) a non-OTA/OTα-producing
Figure Legend Snippet: Selected chromatograms of fungal extracts analysed using HPLC coupled to a fluorescence detector of (a) an OTA/OTα-producing strain ( A . carbonarius A-2034) (OTα retention time: 2.773 min; OTA retention time: 4.890 min) and (b) a non-OTA/OTα-producing

Techniques Used: High Performance Liquid Chromatography, Fluorescence

11) Product Images from "Monoamine Oxidase-A Inhibition and Associated Antioxidant Activity in Plant Extracts with Potential Antidepressant Actions"

Article Title: Monoamine Oxidase-A Inhibition and Associated Antioxidant Activity in Plant Extracts with Potential Antidepressant Actions

Journal: BioMed Research International

doi: 10.1155/2018/4810394

HPLC chromatograms of extracts from H. perforatum flowers. (a) Detection of hypericins at 590 nm. 1 : protopseudohypericin; 2 : pseudohypericin; 3 : protohypericin; and 4 : hypericin. (b) Detection of phenols and flavonoids at 265 nm. 1 : chlorogenic acid; 2 : rutin; 3 : hyperoside; 4 : isoquercitrin; 5 : miquelianin; 6 : acetyl hyperoside; 7 : quercitrin; 8 : quercetin; and 9 : biapigenin. (c) Detection of phloroglucinols at 280 nm. 1 : hyperfirin, 2 : adhyperfirin; 3 : hyperforin; and 4 : adhyperforin.
Figure Legend Snippet: HPLC chromatograms of extracts from H. perforatum flowers. (a) Detection of hypericins at 590 nm. 1 : protopseudohypericin; 2 : pseudohypericin; 3 : protohypericin; and 4 : hypericin. (b) Detection of phenols and flavonoids at 265 nm. 1 : chlorogenic acid; 2 : rutin; 3 : hyperoside; 4 : isoquercitrin; 5 : miquelianin; 6 : acetyl hyperoside; 7 : quercitrin; 8 : quercetin; and 9 : biapigenin. (c) Detection of phloroglucinols at 280 nm. 1 : hyperfirin, 2 : adhyperfirin; 3 : hyperforin; and 4 : adhyperforin.

Techniques Used: High Performance Liquid Chromatography

12) Product Images from "Cezomycin Is Activated by CalC to Its Ester Form for Further Biosynthesis Steps in the Production of Calcimycin in Streptomyces chartreusis NRRL 3882"

Article Title: Cezomycin Is Activated by CalC to Its Ester Form for Further Biosynthesis Steps in the Production of Calcimycin in Streptomyces chartreusis NRRL 3882

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.00586-18

Phenotypic analysis of calC gene involved in the cezomycin modification pathway. HPLC analysis of calcimycin and cezomycin production in wild-type, calC mutant, and calC complementation strains.
Figure Legend Snippet: Phenotypic analysis of calC gene involved in the cezomycin modification pathway. HPLC analysis of calcimycin and cezomycin production in wild-type, calC mutant, and calC complementation strains.

Techniques Used: Modification, High Performance Liquid Chromatography, Mutagenesis

13) Product Images from "Plant Natural Products Calycosin and Gallic Acid Synergistically Attenuate Neutrophil Infiltration and Subsequent Injury in Isoproterenol-Induced Myocardial Infarction: A Possible Role for Leukotriene B4 12-Hydroxydehydrogenase?"

Article Title: Plant Natural Products Calycosin and Gallic Acid Synergistically Attenuate Neutrophil Infiltration and Subsequent Injury in Isoproterenol-Induced Myocardial Infarction: A Possible Role for Leukotriene B4 12-Hydroxydehydrogenase?

Journal: Oxidative Medicine and Cellular Longevity

doi: 10.1155/2015/434052

Bioactivity-guided isolation of the active compounds from Radix Astragali for LTB4DH induction. (a) Schematic illustration of the bioactivity-guided fractionation procedure. The active compounds were isolated from Radix Astragali extract through ethanol precipitation, extraction with organic solvents, and HPLC separation on a C18 column. The active fractions were highlighted with star ( ∗ ) and in bold. (b) RT-PCR detection of LTB4DH induction. The fractions in combination with gallic acid (GA) were assayed by semiquantitative RT-PCR for LTB4DH induction in HepG2 cells. (c) HPLC separation and RT-PCR detection of LTB4DH induction. Nine HPLC fractions in combination with gallic acid (GA) were assayed by semiquantitative RT-PCR for LTB4DH induction in HepG2 cells. The concentration of each fraction was normalized against the concentration of raw Radix Astragali (5.18 mg/mL). Representative HPLC profile and RT-PCR analysis were shown. (d) Chemical identification by HPLC-ESI-MS technique. The MS profiles of fraction 6 and 8 were shown. The structures of calycosin and formononetin were generated by ChemSketch software ( http://www.acdlabs.com ).
Figure Legend Snippet: Bioactivity-guided isolation of the active compounds from Radix Astragali for LTB4DH induction. (a) Schematic illustration of the bioactivity-guided fractionation procedure. The active compounds were isolated from Radix Astragali extract through ethanol precipitation, extraction with organic solvents, and HPLC separation on a C18 column. The active fractions were highlighted with star ( ∗ ) and in bold. (b) RT-PCR detection of LTB4DH induction. The fractions in combination with gallic acid (GA) were assayed by semiquantitative RT-PCR for LTB4DH induction in HepG2 cells. (c) HPLC separation and RT-PCR detection of LTB4DH induction. Nine HPLC fractions in combination with gallic acid (GA) were assayed by semiquantitative RT-PCR for LTB4DH induction in HepG2 cells. The concentration of each fraction was normalized against the concentration of raw Radix Astragali (5.18 mg/mL). Representative HPLC profile and RT-PCR analysis were shown. (d) Chemical identification by HPLC-ESI-MS technique. The MS profiles of fraction 6 and 8 were shown. The structures of calycosin and formononetin were generated by ChemSketch software ( http://www.acdlabs.com ).

Techniques Used: Isolation, Fractionation, Ethanol Precipitation, High Performance Liquid Chromatography, Reverse Transcription Polymerase Chain Reaction, Concentration Assay, Mass Spectrometry, Generated, Software

14) Product Images from "Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains – An Unusual Secondary Metabolite with Various Properties"

Article Title: Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains – An Unusual Secondary Metabolite with Various Properties

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2017.00221

Production of polyketomycin and foxicin in Streptomyces diastatochromogenes Tü6028. (A) HPLC chromatogram of S. diastatochromogenes Tü6028 wild type at λ430 nm with polyketomycin structure (top) and of Δ pokOIV mutant at λ320 nm (below); (B) Production of foxicin A (▴) and polyketomycin (O) in S. diastatochromogenes Tü6028 wild type; (C) Morphology of S. diastatochromogenes Tü6028 wild type (left) and Δ pokOIV mutant (right). On the plate, the Δ pokOIV mutant appears yellow and deficient in producing spores and melanins (dark color).
Figure Legend Snippet: Production of polyketomycin and foxicin in Streptomyces diastatochromogenes Tü6028. (A) HPLC chromatogram of S. diastatochromogenes Tü6028 wild type at λ430 nm with polyketomycin structure (top) and of Δ pokOIV mutant at λ320 nm (below); (B) Production of foxicin A (▴) and polyketomycin (O) in S. diastatochromogenes Tü6028 wild type; (C) Morphology of S. diastatochromogenes Tü6028 wild type (left) and Δ pokOIV mutant (right). On the plate, the Δ pokOIV mutant appears yellow and deficient in producing spores and melanins (dark color).

Techniques Used: High Performance Liquid Chromatography, Mutagenesis

15) Product Images from "Study on the presence of ochratoxin α in cultures of ochratoxigenic and non- ochratoxigenic strains of Aspergillus carbonarius"

Article Title: Study on the presence of ochratoxin α in cultures of ochratoxigenic and non- ochratoxigenic strains of Aspergillus carbonarius

Journal: PLoS ONE

doi: 10.1371/journal.pone.0185986

Selected chromatograms of fungal extracts analysed using HPLC coupled to a fluorescence detector of (a) an OTA/OTα-producing strain ( A . carbonarius A-2034) (OTα retention time: 2.773 min; OTA retention time: 4.890 min) and (b) a non-OTA/OTα-producing strain of ( A . carbonarius A-2160), after incubation at 15°C for 30 days on Czapek Yeast extract Agar.
Figure Legend Snippet: Selected chromatograms of fungal extracts analysed using HPLC coupled to a fluorescence detector of (a) an OTA/OTα-producing strain ( A . carbonarius A-2034) (OTα retention time: 2.773 min; OTA retention time: 4.890 min) and (b) a non-OTA/OTα-producing strain of ( A . carbonarius A-2160), after incubation at 15°C for 30 days on Czapek Yeast extract Agar.

Techniques Used: High Performance Liquid Chromatography, Fluorescence, Incubation

Selected extracted ion chromatograms of fungal extracts and (a) OTA and (b) OTα standards analysed using HPLC-MS and an OTA/OTα-producing strain ( A . carbonarius A-1657) and a non-OTA/OTα-producing strain ( A . carbonarius A-2579), after incubation at 15°C for 30 days on Czapek Yeast extract Agar.
Figure Legend Snippet: Selected extracted ion chromatograms of fungal extracts and (a) OTA and (b) OTα standards analysed using HPLC-MS and an OTA/OTα-producing strain ( A . carbonarius A-1657) and a non-OTA/OTα-producing strain ( A . carbonarius A-2579), after incubation at 15°C for 30 days on Czapek Yeast extract Agar.

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry, Incubation

16) Product Images from "Cezomycin Is Activated by CalC to Its Ester Form for Further Biosynthesis Steps in the Production of Calcimycin in Streptomyces chartreusis NRRL 3882"

Article Title: Cezomycin Is Activated by CalC to Its Ester Form for Further Biosynthesis Steps in the Production of Calcimycin in Streptomyces chartreusis NRRL 3882

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.00586-18

Phenotypic analysis of calC gene involved in the cezomycin modification pathway. HPLC analysis of calcimycin and cezomycin production in wild-type, calC mutant, and calC complementation strains.
Figure Legend Snippet: Phenotypic analysis of calC gene involved in the cezomycin modification pathway. HPLC analysis of calcimycin and cezomycin production in wild-type, calC mutant, and calC complementation strains.

Techniques Used: Modification, High Performance Liquid Chromatography, Mutagenesis

Related Articles

High Performance Liquid Chromatography:

Article Title: Monoamine Oxidase-A Inhibition and Associated Antioxidant Activity in Plant Extracts with Potential Antidepressant Actions
Article Snippet: .. Identification by HPLC-ESI-Mass Spectrometry Identification of compounds in H. perforatum extracts was done by HPLC-MS (electrospray-negative ion mode) by using a 1200 series HPLC-DAD coupled to a 6110 quadrupole-MS (Agilent). ..

Article Title: Beauveria bassiana: a new N6-(2-hydroxyethyl)-adenosine–producing fungus
Article Snippet: .. Verification of HEA production The production of HEA was verified by HPLC-MS. An Agilent 6520 Accurate-Mass Quadrupole Time-of-Flight mass spectrometer (Santa Clara, CA, USA) was equipped with an electrospray ionisation interface. ..

Article Title: Interspecies modulation of bacterial development through iron competition and siderophore piracy
Article Snippet: .. Filtered supernatants from Amycolatopsis sp. AA4 liquid cultures (described above) were analyzed directly by HPLC-MS using an Agilent 1200 Series HPLC system equipped with a diode array detector and a 6130 Series ESI mass spectrometer. .. The supernatants were separated on an analytical Phenomenex Luna C18 column (5 µm, 4.6 × 100 mm) operating at 0.7 mL/min with a gradient of 10 % MeCN in H2 O (+0.1 % formic acid) to 100 % MeCN (+0.1 % formic acid) over 25 min.

Article Title: Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains – An Unusual Secondary Metabolite with Various Properties
Article Snippet: .. Analysis of Foxicin by HPLC/MS For analysis, HPLC-MS equipped with a XBridge C18 (3.5 μm; 20 mm × 4.6 mm) precolumn and a XBridge C18 (3.5 μm; 100 mm × 4.6 mm) main column, an UV/visible light detector and a mass spectrometer (Agilent, 1100 Series) was used. ..

Article Title: Phytochemical Composition, Antioxidant, Antimicrobial and in Vivo Anti-inflammatory Activity of Traditionally Used Romanian Ajuga laxmannii (Murray) Benth. (“Nobleman’s Beard” – Barba Împăratului)
Article Snippet: .. Identification and Quantification of Iridoids Targeted A. laxmannii iridoids (aucubin, catalpol, harpagide, harpagoside, and 8-O -acetylharpagide) were analyzed by HPLC-MS on a Agilent 1100 liquid chromatography system equipped with a binary pump, autosampler, thermostat and detector (all 1100 Series from Agilent Inc., United States). ..

Article Title: Novel Conjugates of 1,3-Diacylglycerol and Lipoic Acid: Synthesis, DPPH Assay, and RP-LC-MS-APCI Analysis
Article Snippet: .. RP-HPLC-MS-APCI An HPLC-MS (HP 1100 Series, Agilent technologies Inc., Palo Alto, CA) equipped with a quaternary gradient pump, thermostated column compartment, thermostated autosampler, single quadrupole mass analyzer (G 1946D), Chemstation Rev.B.04.01 software. .. DOLA and DODHLA were separated using a reversed phase Thermo Hypersil GOLD column, 150 × 4.6 mm i.d, 3 μ m particle size (Thermo Electron Corporation, UK).

Article Title: Phytochemical Composition, Antioxidant, Antimicrobial and in Vivo Anti-inflammatory Activity of Traditionally Used Romanian Ajuga laxmannii (Murray) Benth. (“Nobleman’s Beard” – Barba Împăratului)
Article Snippet: .. Identification and Quantification of Polyphenolic Compounds In order to determine the polyphenolic compounds from A. laxmannii extracts, an optimized HPLC/MS method for the identification and quantification of 18 polyphenols was employed. ..

Article Title: Identification of a novel hydroxylated metabolite of 2,2′,3,5′,6-pentachlorobiphenyl formed in whole poplar plants
Article Snippet: .. Enantioselective analysis of OH-PCB95 was carried out by HPLC-MS (Agilent 1100 Series LCMSD) with chiral column Nucleodex β-PM (4mm × 200mm, 5μm, Macherey-Nagel, Germany). .. MS electrospray in negative ionization mode (LC-ESI (−)-MS) was applied.

Liquid Chromatography:

Article Title: Phytochemical Composition, Antioxidant, Antimicrobial and in Vivo Anti-inflammatory Activity of Traditionally Used Romanian Ajuga laxmannii (Murray) Benth. (“Nobleman’s Beard” – Barba Împăratului)
Article Snippet: .. Identification and Quantification of Iridoids Targeted A. laxmannii iridoids (aucubin, catalpol, harpagide, harpagoside, and 8-O -acetylharpagide) were analyzed by HPLC-MS on a Agilent 1100 liquid chromatography system equipped with a binary pump, autosampler, thermostat and detector (all 1100 Series from Agilent Inc., United States). ..

Mass Spectrometry:

Article Title: Beauveria bassiana: a new N6-(2-hydroxyethyl)-adenosine–producing fungus
Article Snippet: .. Verification of HEA production The production of HEA was verified by HPLC-MS. An Agilent 6520 Accurate-Mass Quadrupole Time-of-Flight mass spectrometer (Santa Clara, CA, USA) was equipped with an electrospray ionisation interface. ..

Article Title: Interspecies modulation of bacterial development through iron competition and siderophore piracy
Article Snippet: .. Filtered supernatants from Amycolatopsis sp. AA4 liquid cultures (described above) were analyzed directly by HPLC-MS using an Agilent 1200 Series HPLC system equipped with a diode array detector and a 6130 Series ESI mass spectrometer. .. The supernatants were separated on an analytical Phenomenex Luna C18 column (5 µm, 4.6 × 100 mm) operating at 0.7 mL/min with a gradient of 10 % MeCN in H2 O (+0.1 % formic acid) to 100 % MeCN (+0.1 % formic acid) over 25 min.

Article Title: Wide Distribution of Foxicin Biosynthetic Gene Clusters in Streptomyces Strains – An Unusual Secondary Metabolite with Various Properties
Article Snippet: .. Analysis of Foxicin by HPLC/MS For analysis, HPLC-MS equipped with a XBridge C18 (3.5 μm; 20 mm × 4.6 mm) precolumn and a XBridge C18 (3.5 μm; 100 mm × 4.6 mm) main column, an UV/visible light detector and a mass spectrometer (Agilent, 1100 Series) was used. ..

Software:

Article Title: Novel Conjugates of 1,3-Diacylglycerol and Lipoic Acid: Synthesis, DPPH Assay, and RP-LC-MS-APCI Analysis
Article Snippet: .. RP-HPLC-MS-APCI An HPLC-MS (HP 1100 Series, Agilent technologies Inc., Palo Alto, CA) equipped with a quaternary gradient pump, thermostated column compartment, thermostated autosampler, single quadrupole mass analyzer (G 1946D), Chemstation Rev.B.04.01 software. .. DOLA and DODHLA were separated using a reversed phase Thermo Hypersil GOLD column, 150 × 4.6 mm i.d, 3 μ m particle size (Thermo Electron Corporation, UK).

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  • 85
    Agilent technologies 1100 capillary hplc ion trap ms system
    <t>HPLC-ESI</t> + -MS/MS analysis of oxazolone in enzymatic hydrolysates of rat liver DNA (300 μg) following off-line HPLC purification ( Scheme 2 ). An Agilent <t>1100</t> series capillary HPLC was interfaced to a Thermo-Finnigan TSQ Quantum Ultra mass spectrometer. A Thermo Hypersil-Keystone Hypercarb column (0.5 × 100 mm, 5 μm) was eluted at a flow rate of 12 μl/min with a gradient of isopropanol/acetonitrile (3:1) (solvent B) in 0.05% acetic acid (solvent A). The spray voltage was set to 3.1 kV, the source temperature was 250°C, and the sheath gas pressure was 30 psi. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 247.1→87.1 [M + 2H − dR − CO 2 ] + , and m/z 251.1→91.1 for oxazolone and 15 N 4 -oxazolone, respectively.
    1100 Capillary Hplc Ion Trap Ms System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1100 capillary hplc ion trap ms system/product/Agilent technologies
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    85
    Agilent technologies hplc q tof ms conditions chromatographic
    Total ion chromatograms (TICs) of rat urine and plasma samples by <t>HPLC/Q-TOF/MS.</t> (a) TIC of the urine sample after oral administration at a single dose of 50 mg/kg BG; (b) TIC of blank urine; (c) TIC of the plasma sample after oral administration at a single dose of 50 mg/kg BG; (d) TIC of blank plasma.
    Hplc Q Tof Ms Conditions Chromatographic, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc q tof ms conditions chromatographic/product/Agilent technologies
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    91
    Agilent technologies hplc ms system
    (a) <t>HPLC</t> chromatograms of reactions between K1 and Ae1 , Ae2 , and Ae3 (pH 8.5, 25 °C, after 1 and 5 h). (b) Electrospray ionization (ESI) mass spectra of cross-linked K1 . (c) Heat map representation of reaction yield, allowing a comparison of relative reaction rates (analytical yields are based on HPLC chromatograms, Figures S2–S4 ).
    Hplc Ms System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc ms system/product/Agilent technologies
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    85
    Agilent technologies hplc dad esi ms ms hplc analysis
    <t>HPLC–ESI-MS</t> total ion chromatogram (TIC) in positive ion mode of (A) the mixed standard and (B) YZT.
    Hplc Dad Esi Ms Ms Hplc Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HPLC-ESI + -MS/MS analysis of oxazolone in enzymatic hydrolysates of rat liver DNA (300 μg) following off-line HPLC purification ( Scheme 2 ). An Agilent 1100 series capillary HPLC was interfaced to a Thermo-Finnigan TSQ Quantum Ultra mass spectrometer. A Thermo Hypersil-Keystone Hypercarb column (0.5 × 100 mm, 5 μm) was eluted at a flow rate of 12 μl/min with a gradient of isopropanol/acetonitrile (3:1) (solvent B) in 0.05% acetic acid (solvent A). The spray voltage was set to 3.1 kV, the source temperature was 250°C, and the sheath gas pressure was 30 psi. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 247.1→87.1 [M + 2H − dR − CO 2 ] + , and m/z 251.1→91.1 for oxazolone and 15 N 4 -oxazolone, respectively.

    Journal: Nucleic Acids Research

    Article Title: Quantitative analysis of the oxidative DNA lesion, 2,2-diamino-4-(2-deoxy-?-d-erythro-pentofuranosyl)amino]-5(2H)-oxazolone (oxazolone), in vitro and in vivo by isotope dilution-capillary HPLC-ESI-MS/MS

    doi: 10.1093/nar/gkl596

    Figure Lengend Snippet: HPLC-ESI + -MS/MS analysis of oxazolone in enzymatic hydrolysates of rat liver DNA (300 μg) following off-line HPLC purification ( Scheme 2 ). An Agilent 1100 series capillary HPLC was interfaced to a Thermo-Finnigan TSQ Quantum Ultra mass spectrometer. A Thermo Hypersil-Keystone Hypercarb column (0.5 × 100 mm, 5 μm) was eluted at a flow rate of 12 μl/min with a gradient of isopropanol/acetonitrile (3:1) (solvent B) in 0.05% acetic acid (solvent A). The spray voltage was set to 3.1 kV, the source temperature was 250°C, and the sheath gas pressure was 30 psi. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 247.1→87.1 [M + 2H − dR − CO 2 ] + , and m/z 251.1→91.1 for oxazolone and 15 N 4 -oxazolone, respectively.

    Article Snippet: Our HPLC-ESI-MS/MS method for 8-oxo-dG employed selected reaction monitoring of the transitions 284.2 [M + H]+ →168.2 [M + 2H − dR]+ (8-oxo-dG) and 289.2 [M + H]+ →173.2 [M + 2H − dR]+ (15 N5 –8-oxo-dG) on an Agilent 1100 capillary HPLC- Ion Trap MS system.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Purification, Flow Cytometry

    HPLC-ESI + -MS/MS analysis of 8-oxo-dG in an enzymatic hydrolysate of rat liver DNA (80 μg). An Agilent 1100 series capillary HPLC-ion trap MS system was used. A Zorbax SB C18 column (0.5 × 150 mm, 5 (m) was maintained at 10°C and eluted at a flow rate of 12 (l/min with a gradient of methanol (solvent B) in 15 mM ammonium acetate (solvent A). The mass spectrometer was operated in the positive ion MS/MS mode. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 284.1→168.0 (M + 2H − dR) + for 8-oxo-dG and the corresponding transition m/z 289.1→173 for [ 15 N 5 ]-8-oxo-dG.

    Journal: Nucleic Acids Research

    Article Title: Quantitative analysis of the oxidative DNA lesion, 2,2-diamino-4-(2-deoxy-?-d-erythro-pentofuranosyl)amino]-5(2H)-oxazolone (oxazolone), in vitro and in vivo by isotope dilution-capillary HPLC-ESI-MS/MS

    doi: 10.1093/nar/gkl596

    Figure Lengend Snippet: HPLC-ESI + -MS/MS analysis of 8-oxo-dG in an enzymatic hydrolysate of rat liver DNA (80 μg). An Agilent 1100 series capillary HPLC-ion trap MS system was used. A Zorbax SB C18 column (0.5 × 150 mm, 5 (m) was maintained at 10°C and eluted at a flow rate of 12 (l/min with a gradient of methanol (solvent B) in 15 mM ammonium acetate (solvent A). The mass spectrometer was operated in the positive ion MS/MS mode. Quantitative analyses were performed in selected reaction monitoring mode using the transitions m/z 284.1→168.0 (M + 2H − dR) + for 8-oxo-dG and the corresponding transition m/z 289.1→173 for [ 15 N 5 ]-8-oxo-dG.

    Article Snippet: Our HPLC-ESI-MS/MS method for 8-oxo-dG employed selected reaction monitoring of the transitions 284.2 [M + H]+ →168.2 [M + 2H − dR]+ (8-oxo-dG) and 289.2 [M + H]+ →173.2 [M + 2H − dR]+ (15 N5 –8-oxo-dG) on an Agilent 1100 capillary HPLC- Ion Trap MS system.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, Flow Cytometry

    Total ion chromatograms (TICs) of rat urine and plasma samples by HPLC/Q-TOF/MS. (a) TIC of the urine sample after oral administration at a single dose of 50 mg/kg BG; (b) TIC of blank urine; (c) TIC of the plasma sample after oral administration at a single dose of 50 mg/kg BG; (d) TIC of blank plasma.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats

    doi: 10.1155/2013/473649

    Figure Lengend Snippet: Total ion chromatograms (TICs) of rat urine and plasma samples by HPLC/Q-TOF/MS. (a) TIC of the urine sample after oral administration at a single dose of 50 mg/kg BG; (b) TIC of blank urine; (c) TIC of the plasma sample after oral administration at a single dose of 50 mg/kg BG; (d) TIC of blank plasma.

    Article Snippet: HPLC/Q-TOF/MS Conditions Chromatographic experiments were performed on an Agilent 1200 series HPLC system, equipped with binary pump, autosampler, on-line degasser and automatic thermostatic column oven (CA, USA).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    (a) HPLC chromatograms of reactions between K1 and Ae1 , Ae2 , and Ae3 (pH 8.5, 25 °C, after 1 and 5 h). (b) Electrospray ionization (ESI) mass spectra of cross-linked K1 . (c) Heat map representation of reaction yield, allowing a comparison of relative reaction rates (analytical yields are based on HPLC chromatograms, Figures S2–S4 ).

    Journal: The Journal of Organic Chemistry

    Article Title: In Situ Cyclization of Proteins (INCYPRO): Cross-Link Derivatization Modulates Protein Stability

    doi: 10.1021/acs.joc.9b02490

    Figure Lengend Snippet: (a) HPLC chromatograms of reactions between K1 and Ae1 , Ae2 , and Ae3 (pH 8.5, 25 °C, after 1 and 5 h). (b) Electrospray ionization (ESI) mass spectra of cross-linked K1 . (c) Heat map representation of reaction yield, allowing a comparison of relative reaction rates (analytical yields are based on HPLC chromatograms, Figures S2–S4 ).

    Article Snippet: Protein and cross-linker identity and purity were confirmed by HPLC/ESI-MS analysis performed in a HPLC-MS system (Agilent Technologies) provided with a Zorbax Eclipse, XDB-C18 reverse-phase column (4.6 × 150 mm, particle size 5 μm, Agilent; solvent A: H2 O + 0.1% TFA; solvent B: acetonitrile +0.1% TFA; flow rate of 1 mL min–1 ).

    Techniques: High Performance Liquid Chromatography

    HPLC–ESI-MS total ion chromatogram (TIC) in positive ion mode of (A) the mixed standard and (B) YZT.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Quantitative and qualitative analysis of common peaks in chemical fingerprint of Yuanhu Zhitong tablet by HPLC-DAD–MS/MS

    doi: 10.1016/j.jpha.2013.12.004

    Figure Lengend Snippet: HPLC–ESI-MS total ion chromatogram (TIC) in positive ion mode of (A) the mixed standard and (B) YZT.

    Article Snippet: 2.2 HPLC-DAD–ESI-MS/MS HPLC analysis was performed on an Agilent 1260 series HPLC system.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry

    Representative HPLC-DAD chromatograms of mixed standard solutions (A) at 254 nm, 270 nm, 280 nm and 345 nm; YZT (B) at 254 nm, 270 nm, 280 nm and 345 nm; the negative sample without Radix Corydalis (C) at 280 nm; and the negative sample without Rhizoma Angelicae dahuricae (D) at 280 nm. (3) protopine; (7) jatrorrhizine; (8) coptisine; (14) palmatine; (15) berberine; (20) xanthotoxin; (23) bergapten; (28) tetrahydropalmatine; (37) imperatorin; (40) isoimperatorin.

    Journal: Journal of Pharmaceutical Analysis

    Article Title: Quantitative and qualitative analysis of common peaks in chemical fingerprint of Yuanhu Zhitong tablet by HPLC-DAD–MS/MS

    doi: 10.1016/j.jpha.2013.12.004

    Figure Lengend Snippet: Representative HPLC-DAD chromatograms of mixed standard solutions (A) at 254 nm, 270 nm, 280 nm and 345 nm; YZT (B) at 254 nm, 270 nm, 280 nm and 345 nm; the negative sample without Radix Corydalis (C) at 280 nm; and the negative sample without Rhizoma Angelicae dahuricae (D) at 280 nm. (3) protopine; (7) jatrorrhizine; (8) coptisine; (14) palmatine; (15) berberine; (20) xanthotoxin; (23) bergapten; (28) tetrahydropalmatine; (37) imperatorin; (40) isoimperatorin.

    Article Snippet: 2.2 HPLC-DAD–ESI-MS/MS HPLC analysis was performed on an Agilent 1260 series HPLC system.

    Techniques: High Performance Liquid Chromatography