hotstartaq master mix kit  (Qiagen)


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    Structured Review

    Qiagen hotstartaq master mix kit
    Hotstartaq Master Mix Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq master mix kit/product/Qiagen
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hotstartaq master mix kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: The amplicon was purified with the NucleoSpin purification kit (Macherey-Nagel, Düren, Germany) and cloned into the pDRIVE vector (Qiagen, Hilden, Germany) using One Shot ® TOP10 Chemically Competent Escherichia coli (Invitrogen, Darmstadt, Germany). .. The real-time PCR reaction was set up in a total volume of 25 l containing 12.5 l of HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany), 10 pmol from the forward and reverse primers, 5 pmol of the Probe, 5 pmol of Rox reference dye and 3 l of the target DNA (Plasmid DNA).

    Amplification:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: The amplicon was purified with the NucleoSpin purification kit (Macherey-Nagel, Düren, Germany) and cloned into the pDRIVE vector (Qiagen, Hilden, Germany) using One Shot ® TOP10 Chemically Competent Escherichia coli (Invitrogen, Darmstadt, Germany). .. The real-time PCR reaction was set up in a total volume of 25 l containing 12.5 l of HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany), 10 pmol from the forward and reverse primers, 5 pmol of the Probe, 5 pmol of Rox reference dye and 3 l of the target DNA (Plasmid DNA).

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: .. Briefly, DNA of the CPV-2 strain vBI 265 was amplified using the HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany) together with the forward and reverse primers. .. The amplicon was purified with the NucleoSpin purification kit (Macherey-Nagel, Düren, Germany) and cloned into the pDRIVE vector (Qiagen, Hilden, Germany) using One Shot ® TOP10 Chemically Competent Escherichia coli (Invitrogen, Darmstadt, Germany).

    Polymerase Chain Reaction:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: The real-time PCR reaction was set up in a total volume of 25 l containing 12.5 l of HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany), 10 pmol from the forward and reverse primers, 5 pmol of the Probe, 5 pmol of Rox reference dye and 3 l of the target DNA (Plasmid DNA). .. The cycling condition consisted of the initial denaturation at 95 • C for 15 min to allow the activation of the polymerase, followed by 40 cycles of denaturation at 95 • C for 30 s, annealing at 58 • C for 30 s and extension at 72 • C for 30 s. The PCR was performed on the Mx3000P platform (Stratagene, La Jolla, USA).

    Quantitation Assay:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: In order to create a standard curve for viral quantitation, a plasmid harboring the target sequence of the real-time PCR was generated. .. Briefly, DNA of the CPV-2 strain vBI 265 was amplified using the HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany) together with the forward and reverse primers.

    Labeling:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: The TaqMan probe was labeled with the fluorescent reporter dye 6-carboxyfluorescein (FAM) at the 5 end and with the BlackBerry Quencher (BBQ) at the 3 end. .. Briefly, DNA of the CPV-2 strain vBI 265 was amplified using the HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany) together with the forward and reverse primers.

    Purification:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: The amplicon was purified with the NucleoSpin purification kit (Macherey-Nagel, Düren, Germany) and cloned into the pDRIVE vector (Qiagen, Hilden, Germany) using One Shot ® TOP10 Chemically Competent Escherichia coli (Invitrogen, Darmstadt, Germany). .. The real-time PCR reaction was set up in a total volume of 25 l containing 12.5 l of HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany), 10 pmol from the forward and reverse primers, 5 pmol of the Probe, 5 pmol of Rox reference dye and 3 l of the target DNA (Plasmid DNA).

    Real-time Polymerase Chain Reaction:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: .. The real-time PCR reaction was set up in a total volume of 25 l containing 12.5 l of HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany), 10 pmol from the forward and reverse primers, 5 pmol of the Probe, 5 pmol of Rox reference dye and 3 l of the target DNA (Plasmid DNA). ..

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: In order to create a standard curve for viral quantitation, a plasmid harboring the target sequence of the real-time PCR was generated. .. Briefly, DNA of the CPV-2 strain vBI 265 was amplified using the HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany) together with the forward and reverse primers.

    Activation Assay:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: The real-time PCR reaction was set up in a total volume of 25 l containing 12.5 l of HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany), 10 pmol from the forward and reverse primers, 5 pmol of the Probe, 5 pmol of Rox reference dye and 3 l of the target DNA (Plasmid DNA). .. The cycling condition consisted of the initial denaturation at 95 • C for 15 min to allow the activation of the polymerase, followed by 40 cycles of denaturation at 95 • C for 30 s, annealing at 58 • C for 30 s and extension at 72 • C for 30 s. The PCR was performed on the Mx3000P platform (Stratagene, La Jolla, USA).

    Generated:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: In order to create a standard curve for viral quantitation, a plasmid harboring the target sequence of the real-time PCR was generated. .. Briefly, DNA of the CPV-2 strain vBI 265 was amplified using the HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany) together with the forward and reverse primers.

    Spectroscopy:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: Plasmid was extracted using the PeqGOLD Plasmid Miniprep Kit I (Peqlab, Erlangen, Germany) and quantified by UV spectroscopy. .. The real-time PCR reaction was set up in a total volume of 25 l containing 12.5 l of HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany), 10 pmol from the forward and reverse primers, 5 pmol of the Probe, 5 pmol of Rox reference dye and 3 l of the target DNA (Plasmid DNA).

    Sequencing:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: In order to create a standard curve for viral quantitation, a plasmid harboring the target sequence of the real-time PCR was generated. .. Briefly, DNA of the CPV-2 strain vBI 265 was amplified using the HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany) together with the forward and reverse primers.

    Plasmid Preparation:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: .. The real-time PCR reaction was set up in a total volume of 25 l containing 12.5 l of HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany), 10 pmol from the forward and reverse primers, 5 pmol of the Probe, 5 pmol of Rox reference dye and 3 l of the target DNA (Plasmid DNA). ..

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: In order to create a standard curve for viral quantitation, a plasmid harboring the target sequence of the real-time PCR was generated. .. Briefly, DNA of the CPV-2 strain vBI 265 was amplified using the HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany) together with the forward and reverse primers.

    Software:

    Article Title: An updated TaqMan real-time PCR for canine and feline parvoviruses.
    Article Snippet: Potential hairpins and self dimerization were calculated using OligoCalc software (Kibbe, 2007) . .. Briefly, DNA of the CPV-2 strain vBI 265 was amplified using the HotStarTaq Master Mix Kit (Qiagen, Hilden, Germany) together with the forward and reverse primers.

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    Qiagen hotstartaq master mix kit
    Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and <t>HotStarTaq</t> was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.
    Hotstartaq Master Mix Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq master mix kit/product/Qiagen
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hotstartaq master mix kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Qiagen hotstartaq plus master mix
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) <t>HotStarTaq</t> Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Hotstartaq Plus Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq plus master mix/product/Qiagen
    Average 99 stars, based on 107 article reviews
    Price from $9.99 to $1999.99
    hotstartaq plus master mix - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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    Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and HotStarTaq was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.

    Journal: Experimental Animals

    Article Title: Zygosity Determination in Hairless Mice by PCR Based onHrhr Gene Analysis

    doi: 10.1538/expanim.62.267

    Figure Lengend Snippet: Analysis of Hr alleles in homozygous (H) and wild-type (W) HR mice. The normal (wild-type) Hr allele is ~19 kbp in length and consists of 19 exons. A total of 15 overlapping PCRs covering the entire Hr coding sequence revealed that an insertion mutation was localized in intron 6 of the Hr x allele. KOD FX neo was used for PCR amplification of regions 2–7, and HotStarTaq was used for PCR of other regions. PCRs shown in gray typeface (1, 2, 4, 6, 9, 12, 14, 15), no difference between homozygous and wild-type HR mice. PCRs shown in black typeface (3, 5, 7, 8, 10, 11, 13), no band or different bands were obtained in homozygous HR mice. Six agarose gel electropherograms show the band patterns of all PCRs. The primer sets used were: (1) F224 and R1151, (2) F193 and R1873, (3) F193 and R2433, (4) F2463 and R3455, (5) F1843 and R2433, (6) F2052 and R2433, (7) F1843 and R2078, (8) F1843 and R1972, (9) F1913 and R2078, (10) F1843 and Int6-R1458, (11) F1843 and Int6-R979, (12) Int6-F1290 and R1972, (13) F1843 and Int6-R850, (14) F1843 and Int6-R642, and (15) F1843 and Int6-R539. The primer sequences and elongation time are shown in Tables 1 and 2 , respectively. The primer positions for long PCR shown in Fig. 2 are also indicated in this figure.

    Article Snippet: The difference between homozygous (Hrx /Hrx ) and wild-type (Hr /Hr ) genomes was determined using multiple PCRs with HotStarTaq (#203443, Qiagen; regions 1, 8–13) or KOD FX neo (KFX-201, TOYOBO, Osaka, Japan; regions 2–7) DNA polymerases.

    Techniques: Mouse Assay, Sequencing, Mutagenesis, Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis

    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Journal: PLoS ONE

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction

    doi: 10.1371/journal.pone.0073408

    Figure Lengend Snippet: Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Article Snippet: Three commercial PCR master mixes, including the AccuPower PCR PreMix (Bioneer, Daejeon, Korea), AmpliTaq Gold 360 Master Mix (Applied Biosystems, Foster City, CA, USA), and HotStarTaq Plus Master Mix (Qiagen, Valencia, CA, USA), were used for the evaluation of the UCNPs effects on the PCR.

    Techniques: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Marker