hotstartaq dna polymerase pcr kit  (Qiagen)


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    Name:
    HotStarTaq DNA Polymerase
    Description:
    For highly specific amplification with minimal optimization Kit contents Qiagen HotStarTaq DNA Polymerase 250U 5U L 10 min at 97C 60 min at 94C Half life 2 to 4 kb min at 72C Extension Rate Genomic DNA and cDNA Sample Recombinant Enzyme Extra A Addition PCR Amplification Reaction Type High PCR Specificity For Highly Specific Amplification with Minimal Optimization Includes HotStarTaq DNA Polymerase 10x PCR Buffer 5x Q Solution 25mM MgCl2 Benefits Minimal optimization requirements High PCR specificity Easy handling and room temperature setup
    Catalog Number:
    203203
    Price:
    159
    Category:
    HotStarTaq DNA Polymerase
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    Structured Review

    Qiagen hotstartaq dna polymerase pcr kit
    HotStarTaq DNA Polymerase
    For highly specific amplification with minimal optimization Kit contents Qiagen HotStarTaq DNA Polymerase 250U 5U L 10 min at 97C 60 min at 94C Half life 2 to 4 kb min at 72C Extension Rate Genomic DNA and cDNA Sample Recombinant Enzyme Extra A Addition PCR Amplification Reaction Type High PCR Specificity For Highly Specific Amplification with Minimal Optimization Includes HotStarTaq DNA Polymerase 10x PCR Buffer 5x Q Solution 25mM MgCl2 Benefits Minimal optimization requirements High PCR specificity Easy handling and room temperature setup
    https://www.bioz.com/result/hotstartaq dna polymerase pcr kit/product/Qiagen
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hotstartaq dna polymerase pcr kit - by Bioz Stars, 2020-04
    99/100 stars

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    Images

    1) Product Images from "CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET"

    Article Title: CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    doi: 10.1152/ajplung.00021.2015

    CXCL8 hypersecretion is not associated with differences in DNA methylation. Genomic DNA was isolated from ASM cells from 3 asthmatic and 3 nonasthmatic individuals and bisulfite converted. Regions of DNA containing CpG sites were PCR amplified and pyrosequenced. A : schematic showing the position of the 8 CXCL8 CpG sites, the PCR primers (gray arrows), and sequencing primers (black arrows). B : percent methylation of the individual CpG sites in ASM cells from both asthmatic and nonasthmatic individuals.
    Figure Legend Snippet: CXCL8 hypersecretion is not associated with differences in DNA methylation. Genomic DNA was isolated from ASM cells from 3 asthmatic and 3 nonasthmatic individuals and bisulfite converted. Regions of DNA containing CpG sites were PCR amplified and pyrosequenced. A : schematic showing the position of the 8 CXCL8 CpG sites, the PCR primers (gray arrows), and sequencing primers (black arrows). B : percent methylation of the individual CpG sites in ASM cells from both asthmatic and nonasthmatic individuals.

    Techniques Used: DNA Methylation Assay, Isolation, Polymerase Chain Reaction, Amplification, Sequencing, Methylation

    Related Articles

    Multiplexing:

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: The PCR included an initial denaturation step of 95 °C for 15 min; 30 cycles of 1 min at 95 °C, 30 s at 66.5 °C and 30 s at 72 °C followed by a final extension at 72 °C for 10 min. 2 μL of the target-specific PCR product from the previous PCR reaction were used as template for a second PCR reaction (Multiplexing PCR). .. Additionally, the mix contained 0.1 mM concentration of each dNTP (Bioline, Luckenwalde, Germany), 0.75 mM MgCl2 , 1X PCR reaction buffer, 0.03 U of HotStarTaq DNA polymerase (Qiagen, Hilden, Germany), 0.4 μM of each primer.

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: The PCR was carried out on a Biorad Thermo cycler 96-well iCycler under the following conditions: an initial denaturation step of 95° for 5 min, followed by 30 cycles of 98 °C for 20 s, 68 °C for 20 s, 72 °C for 20 s, with a final extension at 72 °C for 5 min. A second PCR (Multiplexing) had the following components (50 μl total volume): KAPA HiFi HotStart ReadyMix 1X (Peqlab, Erlangen, Germany), 0.3 μM of each primer and 2 μl of the previous PCR amplicons. .. This PCR was carried out on a Biorad Thermo cycler 96-well iCycler under the following conditions: an initial denaturation step of 95 °C for 5 min, followed by 10 cycles of 98 °C for 20 s, 59 °C for 20 s, 72 °C for 20 s, with a final extension at 72 °C for 7 min. For HotStarTaq DNA polymerase-based amplification, the PCR mix comprised 0.1 mM concentration of each dNTP (Bioline, Luckenwalde, Germany), 0.4 mM MgCl2 , 1X PCR reaction buffer, 0.03 U of HotStarTaq DNA polymerase (Qiagen, Hilden, Germany), 0.4 μM of each primer and 5 ng of the extracted environmental DNA in a total volume of 50 μl.

    Amplification:

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: Paragraph title: Amplification and Sequencing of Legionella -specific 16S rRNA fragment using KAPA HiFi DNA polymerase and HotStarTaq DNA polymerase ... Additionally, the mix contained 0.1 mM concentration of each dNTP (Bioline, Luckenwalde, Germany), 0.75 mM MgCl2 , 1X PCR reaction buffer, 0.03 U of HotStarTaq DNA polymerase (Qiagen, Hilden, Germany), 0.4 μM of each primer.

    Article Title: CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET
    Article Snippet: Primers for PCR amplification and sequencing were designed [PyroMark Assay Design 2.0 software (Qiagen)] and are given in and indicated in . .. PCRs were performed with the HotStarTaq DNA polymerase PCR kit (Qiagen) under the following conditions: 95°C 5 min; 45 cycles of 94°C 30 s; 56°C 30 s; 72°C 30 s; finally 72°C 10 min, except the PCR primers for CpGs 7 and 8, which required an annealing temperature of 52.6°C.

    Article Title: Small-Molecule Inhibition of HIV pre-mRNA Splicing as a Novel Antiretroviral Therapy to Overcome Drug Resistance
    Article Snippet: .. The mixture was aliquoted in a 96-well plate and subjected to PCR amplification using 0.375 U/15 μl of hotStarTaq DNA Polymerase with specific primers (0.3–0.6 μM) using the buffer provided by the manufacturer (QIAGEN). .. The PCR reaction was carried out in a GeneAmp 9700 PCR system.

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. This PCR was carried out on a Biorad Thermo cycler 96-well iCycler under the following conditions: an initial denaturation step of 95 °C for 5 min, followed by 10 cycles of 98 °C for 20 s, 59 °C for 20 s, 72 °C for 20 s, with a final extension at 72 °C for 7 min. For HotStarTaq DNA polymerase-based amplification, the PCR mix comprised 0.1 mM concentration of each dNTP (Bioline, Luckenwalde, Germany), 0.4 mM MgCl2 , 1X PCR reaction buffer, 0.03 U of HotStarTaq DNA polymerase (Qiagen, Hilden, Germany), 0.4 μM of each primer and 5 ng of the extracted environmental DNA in a total volume of 50 μl. .. The PCR included an initial denaturation step of 95 °C for 15 min; 30 cycles of 1 min at 95 °C, 30 s at 66.5 °C and 30 s at 72 °C followed by a final extension at 72 °C for 10 min. 2 μL of the target-specific PCR product from the previous PCR reaction were used as template for a second PCR reaction (Multiplexing PCR).

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. The 16S rRNA sequences had an average substitution rate per base of 0.322% ± 0.277%, which represents a significant accuracy improvement to libraries amplified by HotStarTaq DNA polymerase (0.383% ± 0.275%) (T -test, P < 0.05). ..

    Article Title: Molecular Detection of Multiple Emerging Pathogens in Sputa from Cystic Fibrosis Patients
    Article Snippet: Paragraph title: Genomic amplification ... The PCR reaction mixture (final volume, 50 µL) contained 5 µL of dNTP (2 mM of each nucleotide), 5 µL of 10× DNA polymerase buffer (QIAGEN, Courtaboeuf, France), 2 µL of MgCl2 (25 mM), 0.25 µL of HotStarTaq DNA polymerase (1.25 U)(QIAGEN, Courtaboeuf, France), 1 µL of each primer 536F, rp2 (10 pmol/µL), and 5 µL of extracted DNA.

    Agarose Gel Electrophoresis:

    Article Title: CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET
    Article Snippet: PCRs were performed with the HotStarTaq DNA polymerase PCR kit (Qiagen) under the following conditions: 95°C 5 min; 45 cycles of 94°C 30 s; 56°C 30 s; 72°C 30 s; finally 72°C 10 min, except the PCR primers for CpGs 7 and 8, which required an annealing temperature of 52.6°C. .. Amplification success was assessed by agarose gel electrophoresis and the resulting products were pyrosequenced with the Pyromark Q24 System (Qiagen).

    Purification:

    Article Title: Polymorphism in the Tyrosine Hydroxylase (TH) Gene Is Associated with Activity-Impulsivity in German Shepherd Dogs
    Article Snippet: DNA sampling and genotyping Buccal smears were collected, and DNA was isolated with the Gentra purification kit (Valencia, CA). .. Shortly: The PCR reaction mixture contained 1 µM of each primer, approximately 5 ng of DNA template, 200 µM dATP, dCTP, dTTP, 100 µM of dGTP and dITP, 0.025 U HotStarTaq DNA polymerase 1x buffer and 1x Q-solution supplied by the Qiagen HotStarTaq polymerase kit in a 10 µl final volume.

    Article Title: Molecular Detection of Multiple Emerging Pathogens in Sputa from Cystic Fibrosis Patients
    Article Snippet: The PCR reaction mixture (final volume, 50 µL) contained 5 µL of dNTP (2 mM of each nucleotide), 5 µL of 10× DNA polymerase buffer (QIAGEN, Courtaboeuf, France), 2 µL of MgCl2 (25 mM), 0.25 µL of HotStarTaq DNA polymerase (1.25 U)(QIAGEN, Courtaboeuf, France), 1 µL of each primer 536F, rp2 (10 pmol/µL), and 5 µL of extracted DNA. .. Then PCR products were purified using the NucleoFast® 96 PCR Kit (MACHEREY-NAGEL, Hoerdt, France) according to the manufacturer's instructions.

    Ligation:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: The optimized singleplex PCR reaction was performed in a final volume of 24 μl, which included 12 μl of 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic), 0.0625 µM of universal FW primer, 0.25 µM of BODIPY-TMRX-labeled REV primer (Table ), and 6 μl of ligation product. .. Several master mixes were tested in the optimization experiments, namely AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA), AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA), OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA), Platinum Hot Start PCR Master Mix (Invitrogen, California, USA), and HotStarTaq DNA Polymerase (Qiagen, Germany).

    Software:

    Article Title: CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET
    Article Snippet: Primers for PCR amplification and sequencing were designed [PyroMark Assay Design 2.0 software (Qiagen)] and are given in and indicated in . .. PCRs were performed with the HotStarTaq DNA polymerase PCR kit (Qiagen) under the following conditions: 95°C 5 min; 45 cycles of 94°C 30 s; 56°C 30 s; 72°C 30 s; finally 72°C 10 min, except the PCR primers for CpGs 7 and 8, which required an annealing temperature of 52.6°C.

    Isolation:

    Article Title: Polymorphism in the Tyrosine Hydroxylase (TH) Gene Is Associated with Activity-Impulsivity in German Shepherd Dogs
    Article Snippet: DNA sampling and genotyping Buccal smears were collected, and DNA was isolated with the Gentra purification kit (Valencia, CA). .. Shortly: The PCR reaction mixture contained 1 µM of each primer, approximately 5 ng of DNA template, 200 µM dATP, dCTP, dTTP, 100 µM of dGTP and dITP, 0.025 U HotStarTaq DNA polymerase 1x buffer and 1x Q-solution supplied by the Qiagen HotStarTaq polymerase kit in a 10 µl final volume.

    Next-Generation Sequencing:

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. Sequence accuracy and quality of generated NGS libraries In order to minimise error rate, validation of our deep sequencing approach was performed with proofreading DNA polymerase KAPA HiFi and compared with the extensively used HotStarTaq DNA polymerase. ..

    Polymerase Chain Reaction:

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. Additionally, the mix contained 0.1 mM concentration of each dNTP (Bioline, Luckenwalde, Germany), 0.75 mM MgCl2 , 1X PCR reaction buffer, 0.03 U of HotStarTaq DNA polymerase (Qiagen, Hilden, Germany), 0.4 μM of each primer. ..

    Article Title: CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET
    Article Snippet: .. PCRs were performed with the HotStarTaq DNA polymerase PCR kit (Qiagen) under the following conditions: 95°C 5 min; 45 cycles of 94°C 30 s; 56°C 30 s; 72°C 30 s; finally 72°C 10 min, except the PCR primers for CpGs 7 and 8, which required an annealing temperature of 52.6°C. .. Amplification success was assessed by agarose gel electrophoresis and the resulting products were pyrosequenced with the Pyromark Q24 System (Qiagen).

    Article Title: Polymorphism in the Tyrosine Hydroxylase (TH) Gene Is Associated with Activity-Impulsivity in German Shepherd Dogs
    Article Snippet: .. Shortly: The PCR reaction mixture contained 1 µM of each primer, approximately 5 ng of DNA template, 200 µM dATP, dCTP, dTTP, 100 µM of dGTP and dITP, 0.025 U HotStarTaq DNA polymerase 1x buffer and 1x Q-solution supplied by the Qiagen HotStarTaq polymerase kit in a 10 µl final volume. .. Conditions of the PCR cycle and the separation of PCR products by gel electrophoreses were as described before .

    Article Title: Touchdown Enzyme Time Release-PCR for Detection and Identification of Chlamydia trachomatis, C. pneumoniae, and C. psittaci Using the 16S and 16S-23S Spacer rRNA Genes
    Article Snippet: For comparison purposes, the analytical sensitivities of primer sets CTR 70/71, CPN 90/91, and CPS 100/101 were also determined with a previously described PCR protocol ( ) which did not use a TETR protocol. .. In addition, analytical sensitivity with spiked clinical specimens were tested by use of the TETR-PCR protocol described above but with an alternative DNA polymerase (2 U of HotStarTaq DNA polymerase; Qiagen, Valencia, Calif.) and 1.5 mM MgCl2 .

    Article Title: Small-Molecule Inhibition of HIV pre-mRNA Splicing as a Novel Antiretroviral Therapy to Overcome Drug Resistance
    Article Snippet: .. The mixture was aliquoted in a 96-well plate and subjected to PCR amplification using 0.375 U/15 μl of hotStarTaq DNA Polymerase with specific primers (0.3–0.6 μM) using the buffer provided by the manufacturer (QIAGEN). .. The PCR reaction was carried out in a GeneAmp 9700 PCR system.

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. This PCR was carried out on a Biorad Thermo cycler 96-well iCycler under the following conditions: an initial denaturation step of 95 °C for 5 min, followed by 10 cycles of 98 °C for 20 s, 59 °C for 20 s, 72 °C for 20 s, with a final extension at 72 °C for 7 min. For HotStarTaq DNA polymerase-based amplification, the PCR mix comprised 0.1 mM concentration of each dNTP (Bioline, Luckenwalde, Germany), 0.4 mM MgCl2 , 1X PCR reaction buffer, 0.03 U of HotStarTaq DNA polymerase (Qiagen, Hilden, Germany), 0.4 μM of each primer and 5 ng of the extracted environmental DNA in a total volume of 50 μl. .. The PCR included an initial denaturation step of 95 °C for 15 min; 30 cycles of 1 min at 95 °C, 30 s at 66.5 °C and 30 s at 72 °C followed by a final extension at 72 °C for 10 min. 2 μL of the target-specific PCR product from the previous PCR reaction were used as template for a second PCR reaction (Multiplexing PCR).

    Article Title: Molecular Detection of Multiple Emerging Pathogens in Sputa from Cystic Fibrosis Patients
    Article Snippet: .. The PCR reaction mixture (final volume, 50 µL) contained 5 µL of dNTP (2 mM of each nucleotide), 5 µL of 10× DNA polymerase buffer (QIAGEN, Courtaboeuf, France), 2 µL of MgCl2 (25 mM), 0.25 µL of HotStarTaq DNA polymerase (1.25 U)(QIAGEN, Courtaboeuf, France), 1 µL of each primer 536F, rp2 (10 pmol/µL), and 5 µL of extracted DNA. ..

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: .. Several master mixes were tested in the optimization experiments, namely AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA), AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA), OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA), Platinum Hot Start PCR Master Mix (Invitrogen, California, USA), and HotStarTaq DNA Polymerase (Qiagen, Germany). .. Each singleplex PCR reaction and thermal cycling protocol was run according to the relevant instructions from the manufacturer.

    Generated:

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. Sequence accuracy and quality of generated NGS libraries In order to minimise error rate, validation of our deep sequencing approach was performed with proofreading DNA polymerase KAPA HiFi and compared with the extensively used HotStarTaq DNA polymerase. ..

    Hot Start PCR:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: .. Several master mixes were tested in the optimization experiments, namely AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA), AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA), OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA), Platinum Hot Start PCR Master Mix (Invitrogen, California, USA), and HotStarTaq DNA Polymerase (Qiagen, Germany). .. Each singleplex PCR reaction and thermal cycling protocol was run according to the relevant instructions from the manufacturer.

    Concentration Assay:

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. Additionally, the mix contained 0.1 mM concentration of each dNTP (Bioline, Luckenwalde, Germany), 0.75 mM MgCl2 , 1X PCR reaction buffer, 0.03 U of HotStarTaq DNA polymerase (Qiagen, Hilden, Germany), 0.4 μM of each primer. ..

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. This PCR was carried out on a Biorad Thermo cycler 96-well iCycler under the following conditions: an initial denaturation step of 95 °C for 5 min, followed by 10 cycles of 98 °C for 20 s, 59 °C for 20 s, 72 °C for 20 s, with a final extension at 72 °C for 7 min. For HotStarTaq DNA polymerase-based amplification, the PCR mix comprised 0.1 mM concentration of each dNTP (Bioline, Luckenwalde, Germany), 0.4 mM MgCl2 , 1X PCR reaction buffer, 0.03 U of HotStarTaq DNA polymerase (Qiagen, Hilden, Germany), 0.4 μM of each primer and 5 ng of the extracted environmental DNA in a total volume of 50 μl. .. The PCR included an initial denaturation step of 95 °C for 15 min; 30 cycles of 1 min at 95 °C, 30 s at 66.5 °C and 30 s at 72 °C followed by a final extension at 72 °C for 10 min. 2 μL of the target-specific PCR product from the previous PCR reaction were used as template for a second PCR reaction (Multiplexing PCR).

    Quantitation Assay:

    Article Title: Small-Molecule Inhibition of HIV pre-mRNA Splicing as a Novel Antiretroviral Therapy to Overcome Drug Resistance
    Article Snippet: The mixture was aliquoted in a 96-well plate and subjected to PCR amplification using 0.375 U/15 μl of hotStarTaq DNA Polymerase with specific primers (0.3–0.6 μM) using the buffer provided by the manufacturer (QIAGEN). .. PCR products were fractionated on a LabChip HT DNA assay station (Caliper) for quantitation and sizing.

    other:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: HotStarTaq DNA Polymerase, on the other hand, reached the highest MFI values.

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: In order to find a balance, the performance of six different master mixes was tested (Fig. ), including the previously utilized HotStarTaq DNA Polymerase – and the upgraded AmpliTaq Gold 360 Master Mix , .

    T-Test:

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. The 16S rRNA sequences had an average substitution rate per base of 0.322% ± 0.277%, which represents a significant accuracy improvement to libraries amplified by HotStarTaq DNA polymerase (0.383% ± 0.275%) (T -test, P < 0.05). ..

    Sequencing:

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: Paragraph title: Amplification and Sequencing of Legionella -specific 16S rRNA fragment using KAPA HiFi DNA polymerase and HotStarTaq DNA polymerase ... Additionally, the mix contained 0.1 mM concentration of each dNTP (Bioline, Luckenwalde, Germany), 0.75 mM MgCl2 , 1X PCR reaction buffer, 0.03 U of HotStarTaq DNA polymerase (Qiagen, Hilden, Germany), 0.4 μM of each primer.

    Article Title: CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET
    Article Snippet: Primers for PCR amplification and sequencing were designed [PyroMark Assay Design 2.0 software (Qiagen)] and are given in and indicated in . .. PCRs were performed with the HotStarTaq DNA polymerase PCR kit (Qiagen) under the following conditions: 95°C 5 min; 45 cycles of 94°C 30 s; 56°C 30 s; 72°C 30 s; finally 72°C 10 min, except the PCR primers for CpGs 7 and 8, which required an annealing temperature of 52.6°C.

    Article Title: Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems
    Article Snippet: .. Sequence accuracy and quality of generated NGS libraries In order to minimise error rate, validation of our deep sequencing approach was performed with proofreading DNA polymerase KAPA HiFi and compared with the extensively used HotStarTaq DNA polymerase. ..

    Sampling:

    Article Title: Polymorphism in the Tyrosine Hydroxylase (TH) Gene Is Associated with Activity-Impulsivity in German Shepherd Dogs
    Article Snippet: Paragraph title: DNA sampling and genotyping ... Shortly: The PCR reaction mixture contained 1 µM of each primer, approximately 5 ng of DNA template, 200 µM dATP, dCTP, dTTP, 100 µM of dGTP and dITP, 0.025 U HotStarTaq DNA polymerase 1x buffer and 1x Q-solution supplied by the Qiagen HotStarTaq polymerase kit in a 10 µl final volume.

    Pyromark Assay:

    Article Title: CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET
    Article Snippet: Primers for PCR amplification and sequencing were designed [PyroMark Assay Design 2.0 software (Qiagen)] and are given in and indicated in . .. PCRs were performed with the HotStarTaq DNA polymerase PCR kit (Qiagen) under the following conditions: 95°C 5 min; 45 cycles of 94°C 30 s; 56°C 30 s; 72°C 30 s; finally 72°C 10 min, except the PCR primers for CpGs 7 and 8, which required an annealing temperature of 52.6°C.

    Hybridization:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: The thermal cycling program consisted of initial denaturation at 95 °C for 2 min, followed by 40 cycles of 95 °C for 15 s, 60 °C for 15 s, and 72 °C for 15 s. Reactions were cooled to 10 °C and either used immediately in the microsphere hybridization step or stored at 4 °C pending hybridization. .. Several master mixes were tested in the optimization experiments, namely AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA), AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA), OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA), Platinum Hot Start PCR Master Mix (Invitrogen, California, USA), and HotStarTaq DNA Polymerase (Qiagen, Germany).

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  • 99
    Qiagen hotstartaq dna polymerase pcr kit
    CXCL8 hypersecretion is not associated with differences in <t>DNA</t> methylation. Genomic DNA was isolated from ASM cells from 3 asthmatic and 3 nonasthmatic individuals and bisulfite converted. Regions of DNA containing CpG sites were <t>PCR</t> amplified and pyrosequenced. A : schematic showing the position of the 8 CXCL8 CpG sites, the PCR primers (gray arrows), and sequencing primers (black arrows). B : percent methylation of the individual CpG sites in ASM cells from both asthmatic and nonasthmatic individuals.
    Hotstartaq Dna Polymerase Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstartaq dna polymerase pcr kit/product/Qiagen
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hotstartaq dna polymerase pcr kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Qiagen multiplex pcr kit
    Electropherogram of singleplex <t>PCR</t> products using Kapa 2GFast HotStart <t>DNA</t> polymerase. Singleplex PCR was performed with varied extension periods (15 s, 30 s, 60 s and 120 s) and with varied Mg 2+ concentrations (3.0 mM and 4.5 mM) using three pairs of locus-specific primers. The designed amplicon size is depicted below each lane.
    Multiplex Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1027 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplex pcr kit/product/Qiagen
    Average 99 stars, based on 1027 article reviews
    Price from $9.99 to $1999.99
    multiplex pcr kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Qiagen hotstarttaq plus master mix kit
    Electropherogram of singleplex <t>PCR</t> products using Kapa 2GFast HotStart <t>DNA</t> polymerase. Singleplex PCR was performed with varied extension periods (15 s, 30 s, 60 s and 120 s) and with varied Mg 2+ concentrations (3.0 mM and 4.5 mM) using three pairs of locus-specific primers. The designed amplicon size is depicted below each lane.
    Hotstarttaq Plus Master Mix Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstarttaq plus master mix kit/product/Qiagen
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    hotstarttaq plus master mix kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    CXCL8 hypersecretion is not associated with differences in DNA methylation. Genomic DNA was isolated from ASM cells from 3 asthmatic and 3 nonasthmatic individuals and bisulfite converted. Regions of DNA containing CpG sites were PCR amplified and pyrosequenced. A : schematic showing the position of the 8 CXCL8 CpG sites, the PCR primers (gray arrows), and sequencing primers (black arrows). B : percent methylation of the individual CpG sites in ASM cells from both asthmatic and nonasthmatic individuals.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET

    doi: 10.1152/ajplung.00021.2015

    Figure Lengend Snippet: CXCL8 hypersecretion is not associated with differences in DNA methylation. Genomic DNA was isolated from ASM cells from 3 asthmatic and 3 nonasthmatic individuals and bisulfite converted. Regions of DNA containing CpG sites were PCR amplified and pyrosequenced. A : schematic showing the position of the 8 CXCL8 CpG sites, the PCR primers (gray arrows), and sequencing primers (black arrows). B : percent methylation of the individual CpG sites in ASM cells from both asthmatic and nonasthmatic individuals.

    Article Snippet: PCRs were performed with the HotStarTaq DNA polymerase PCR kit (Qiagen) under the following conditions: 95°C 5 min; 45 cycles of 94°C 30 s; 56°C 30 s; 72°C 30 s; finally 72°C 10 min, except the PCR primers for CpGs 7 and 8, which required an annealing temperature of 52.6°C.

    Techniques: DNA Methylation Assay, Isolation, Polymerase Chain Reaction, Amplification, Sequencing, Methylation

    Electropherogram of singleplex PCR products using Kapa 2GFast HotStart DNA polymerase. Singleplex PCR was performed with varied extension periods (15 s, 30 s, 60 s and 120 s) and with varied Mg 2+ concentrations (3.0 mM and 4.5 mM) using three pairs of locus-specific primers. The designed amplicon size is depicted below each lane.

    Journal: PLoS ONE

    Article Title: Highly Parallel and Short-Acting Amplification with Locus-Specific Primers to Detect Single Nucleotide Polymorphisms by the DigiTag2 Assay

    doi: 10.1371/journal.pone.0029967

    Figure Lengend Snippet: Electropherogram of singleplex PCR products using Kapa 2GFast HotStart DNA polymerase. Singleplex PCR was performed with varied extension periods (15 s, 30 s, 60 s and 120 s) and with varied Mg 2+ concentrations (3.0 mM and 4.5 mM) using three pairs of locus-specific primers. The designed amplicon size is depicted below each lane.

    Article Snippet: Multiplex PCR with QIAGEN Multiplex PCR Kit Multiplex PCR mix had a final volume of 10 µl, including 10 ng of genomic DNA, 25 nM each primer, 1× Multiplex PCR Buffer (including 3.0 mM Mg2+ ), 0.2 mM dNTPs and HotStarTaq DNA polymerase (QIAGEN Multiplex PCR Kit; QIAGEN, Valencia, CA, USA).

    Techniques: Polymerase Chain Reaction, Amplification