hotstar plus taq polymerase system  (Qiagen)


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    Structured Review

    Qiagen hotstar plus taq polymerase system
    Hotstar Plus Taq Polymerase System, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstar plus taq polymerase system/product/Qiagen
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hotstar plus taq polymerase system - by Bioz Stars, 2020-04
    86/100 stars

    Related Products / Commonly Used Together

    pcr reactions
    mastercycler ep gradient s instrument

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    Related Articles

    Functional Assay:

    Article Title: Linkage between Increased Nociception and Olfaction via a SCN9A Haplotype
    Article Snippet: Genotyping for the functional variants SCN9A rs41268673C > A and rs6746030C > T was done from genomic DNA by means of Pyrosequencing™ assays on a PSQ 96 MA System (Qiagen, Hilden, Germany) using the PyroMark Gold Q96 Reagents set (Qiagen, Hilden, Germany). .. PCR reactions were performed in a 25 µl assay volume on a Mastercycler ep gradient S instrument (Eppendorf, Hamburg, Germany), using the HotStar plus Taq Polymerase system (Qiagen, Hilden, Germany) and SNP-specific PCR primers (for rs41268673 forward primer: 5′-GCGACGCAGCAGTAACATCAG-3′ and reverse primer: 5′-biotin- TGTAAAACGTCCTTACGCTGTCA-3′; for rs6746030: forward primer: 5′-TTTGGTTGAGGGAGTATCACAGAA-3′ and reverse primer: 5′-biotin-TTGTAGCAGGTTTTCCTGATGTTC-3′, respectively).

    Produced:

    Article Title: Linkage between Increased Nociception and Olfaction via a SCN9A Haplotype
    Article Snippet: Furthermore, Nav 1.7 1150W produced hyperexcitability when expressed in rat dorsal root ganglion neurons .The other variant, rs41268673C > A, causes an amino acid exchange at position 610 from proline to threonine, however, its molecular consequences are unknown yet. .. PCR reactions were performed in a 25 µl assay volume on a Mastercycler ep gradient S instrument (Eppendorf, Hamburg, Germany), using the HotStar plus Taq Polymerase system (Qiagen, Hilden, Germany) and SNP-specific PCR primers (for rs41268673 forward primer: 5′-GCGACGCAGCAGTAACATCAG-3′ and reverse primer: 5′-biotin- TGTAAAACGTCCTTACGCTGTCA-3′; for rs6746030: forward primer: 5′-TTTGGTTGAGGGAGTATCACAGAA-3′ and reverse primer: 5′-biotin-TTGTAGCAGGTTTTCCTGATGTTC-3′, respectively).

    Polymerase Chain Reaction:

    Article Title: Linkage between Increased Nociception and Olfaction via a SCN9A Haplotype
    Article Snippet: .. PCR reactions were performed in a 25 µl assay volume on a Mastercycler ep gradient S instrument (Eppendorf, Hamburg, Germany), using the HotStar plus Taq Polymerase system (Qiagen, Hilden, Germany) and SNP-specific PCR primers (for rs41268673 forward primer: 5′-GCGACGCAGCAGTAACATCAG-3′ and reverse primer: 5′-biotin- TGTAAAACGTCCTTACGCTGTCA-3′; for rs6746030: forward primer: 5′-TTTGGTTGAGGGAGTATCACAGAA-3′ and reverse primer: 5′-biotin-TTGTAGCAGGTTTTCCTGATGTTC-3′, respectively). .. The PCR was done with an initial denaturation step for 5 min at 95°C, 45 cycles with a 30 second denaturation step at 95°C, an annealing step at primer-specific temperatures (56°C for rs41268673C > A and 58°C for rs6746030C > T) for 30 s and an elongation step at 72°C for 30 seconds, followed by a final elongation step at 72°C for 5 min.

    Article Title: Consequences of a Human TRPA1 Genetic Variant on the Perception of Nociceptive and Olfactory Stimuli
    Article Snippet: .. PCR reactions were performed in a 25 µl assay volume on a Mastercycler ep gradient S instrument (Eppendorf, Hamburg, Germany), using the HotStar plus Taq Polymerase system (Qiagen, Hilden, Germany) and SNP-specific PCR primers (forward primer: 5′-TAAGTGAGCCAAGTTCAGATCAGA-3′ and reverse primer: 5′-biotin-TTTCACAGAAAGTGAGGTGTTGTA-3′). .. The PCR was done with an initial denaturation step for 5 min at 95°C, 50 cycles with a 30 second denaturation step at 95°C, an annealing step at 45°C for 30 seconds and an elongation step at 72°C for 30 seconds, followed by a final elongation step at 72°C for 5 min.

    Sequencing:

    Article Title: Linkage between Increased Nociception and Olfaction via a SCN9A Haplotype
    Article Snippet: PCR reactions were performed in a 25 µl assay volume on a Mastercycler ep gradient S instrument (Eppendorf, Hamburg, Germany), using the HotStar plus Taq Polymerase system (Qiagen, Hilden, Germany) and SNP-specific PCR primers (for rs41268673 forward primer: 5′-GCGACGCAGCAGTAACATCAG-3′ and reverse primer: 5′-biotin- TGTAAAACGTCCTTACGCTGTCA-3′; for rs6746030: forward primer: 5′-TTTGGTTGAGGGAGTATCACAGAA-3′ and reverse primer: 5′-biotin-TTGTAGCAGGTTTTCCTGATGTTC-3′, respectively). .. The PCR products (25 µl) were used in the Pyrosequencing analyses as previously described with the sequencing primers 5′- CCAAGCCAGTAGGTCC-3′ for rs41268673C > A and 5′-CTTTCTTGTCAGGTTGTG-3′ for rs6746030C > T, respectively.

    Article Title: Consequences of a Human TRPA1 Genetic Variant on the Perception of Nociceptive and Olfactory Stimuli
    Article Snippet: PCR reactions were performed in a 25 µl assay volume on a Mastercycler ep gradient S instrument (Eppendorf, Hamburg, Germany), using the HotStar plus Taq Polymerase system (Qiagen, Hilden, Germany) and SNP-specific PCR primers (forward primer: 5′-TAAGTGAGCCAAGTTCAGATCAGA-3′ and reverse primer: 5′-biotin-TTTCACAGAAAGTGAGGTGTTGTA-3′). .. The PCR product (25 µl) was used in the Pyrosequencing analysis as described previously with the sequencing primer 5′-TGATCCTTCTTTTCTCAGTA-3′ .

    Variant Assay:

    Article Title: Linkage between Increased Nociception and Olfaction via a SCN9A Haplotype
    Article Snippet: Furthermore, Nav 1.7 1150W produced hyperexcitability when expressed in rat dorsal root ganglion neurons .The other variant, rs41268673C > A, causes an amino acid exchange at position 610 from proline to threonine, however, its molecular consequences are unknown yet. .. PCR reactions were performed in a 25 µl assay volume on a Mastercycler ep gradient S instrument (Eppendorf, Hamburg, Germany), using the HotStar plus Taq Polymerase system (Qiagen, Hilden, Germany) and SNP-specific PCR primers (for rs41268673 forward primer: 5′-GCGACGCAGCAGTAACATCAG-3′ and reverse primer: 5′-biotin- TGTAAAACGTCCTTACGCTGTCA-3′; for rs6746030: forward primer: 5′-TTTGGTTGAGGGAGTATCACAGAA-3′ and reverse primer: 5′-biotin-TTGTAGCAGGTTTTCCTGATGTTC-3′, respectively).

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  • 99
    Qiagen hotstart taq dna polymerase
    Effect of individual constituents of the PCR on background fluorescence in the presence or absence of CABS. Each column represents the result of three PCRs performed: 1, in the absence of <t>Taq</t> Gold polymerase, but in the presence of oligonucleotides and template; 2, in the absence of Taq Gold polymerase and template, but in the presence of oligonucleotides; 3, in the absence of Taq Gold polymerase, template, and one oligonucleotide but in the presence of one oligonucleotide; 4, in the absence of primers and template, but in the presence of Taq Gold polymerase; 5, in the absence of template, but in the presence of both primers and polymerase; 6, as in case 3, except in the presence of the alternative primer of the primer combination; 7, in which specific amplicon is produced; and 8, in which no amplicon is expected. Conditions 1, 2, and 6 show the effect of CABS in reducing the background fluorescence caused by the single-strand oligonucleotides. The reaction with CABS in conditions 5 and 8 shows the residual effect caused by primer/dimers, in the case of 8 with additional template <t>DNA.</t>
    Hotstart Taq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstart taq dna polymerase/product/Qiagen
    Average 99 stars, based on 110 article reviews
    Price from $9.99 to $1999.99
    hotstart taq dna polymerase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Qiagen dna polymerase
    TALEN-induced mutations in the Platynereis estrogen receptor . (A) Schematic of the er locus showing target exons 2 and 3 with TALEN target sites (yellow). Blue arrows, primer positions; red double-ended arrow, region of sequence deleted in E; green, <t>DNA-binding</t> domain. Primer combinations used for screening are shown above in B–E. (B–E) <t>PCR,</t> undigested PCR product; NI, non-injected. (B) Restriction digest screening of larvae injected with er Ex3_L2/R2 TALENs (mRNA concentration: 267 ng/µl/TALEN mRNA). Arrowhead indicates uncut PCR product following AflI II digestion (asterisk). (C) Mutation evidence at exon 2 site: uncut band adult worm +3 vs. fully digested product from mutation-negative (−) TALEN-injected worm. (D) Adult worms er+31 and er+37 with mutations at exon 3 site. (E) Deletions (red arrow) detected in larvae and adult worms resulting from simultaneous cleavage at exons 2 and 3 using 300 ng/µl/TALEN mRNA: deletion positive (+); deletion negative (−). Please note different primer pairs used for larval vs. adult samples. (F) Mutant sequences obtained from digest screening for exons 1, 2, and long deletions. Numbers in brackets indicate the sample or worm from which the sequence was obtained; all other sequences are from injected larvae shown in B. Length of mutations are indicated by ∆ with “−” indicating deletions and “+” indicating insertions. Restriction site is shown in boldface type; asterisks indicate frameshift mutations. Shading key: yellow, TALEN binding sites; gray, spacer; blue, nucleotides differing from wild type; green, inserted nucleotides.
    Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase/product/Qiagen
    Average 99 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    dna polymerase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Qiagen hotstar taq master mix kit
    TALEN-induced mutations in the Platynereis estrogen receptor . (A) Schematic of the er locus showing target exons 2 and 3 with TALEN target sites (yellow). Blue arrows, primer positions; red double-ended arrow, region of sequence deleted in E; green, <t>DNA-binding</t> domain. Primer combinations used for screening are shown above in B–E. (B–E) <t>PCR,</t> undigested PCR product; NI, non-injected. (B) Restriction digest screening of larvae injected with er Ex3_L2/R2 TALENs (mRNA concentration: 267 ng/µl/TALEN mRNA). Arrowhead indicates uncut PCR product following AflI II digestion (asterisk). (C) Mutation evidence at exon 2 site: uncut band adult worm +3 vs. fully digested product from mutation-negative (−) TALEN-injected worm. (D) Adult worms er+31 and er+37 with mutations at exon 3 site. (E) Deletions (red arrow) detected in larvae and adult worms resulting from simultaneous cleavage at exons 2 and 3 using 300 ng/µl/TALEN mRNA: deletion positive (+); deletion negative (−). Please note different primer pairs used for larval vs. adult samples. (F) Mutant sequences obtained from digest screening for exons 1, 2, and long deletions. Numbers in brackets indicate the sample or worm from which the sequence was obtained; all other sequences are from injected larvae shown in B. Length of mutations are indicated by ∆ with “−” indicating deletions and “+” indicating insertions. Restriction site is shown in boldface type; asterisks indicate frameshift mutations. Shading key: yellow, TALEN binding sites; gray, spacer; blue, nucleotides differing from wild type; green, inserted nucleotides.
    Hotstar Taq Master Mix Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotstar taq master mix kit/product/Qiagen
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    hotstar taq master mix kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effect of individual constituents of the PCR on background fluorescence in the presence or absence of CABS. Each column represents the result of three PCRs performed: 1, in the absence of Taq Gold polymerase, but in the presence of oligonucleotides and template; 2, in the absence of Taq Gold polymerase and template, but in the presence of oligonucleotides; 3, in the absence of Taq Gold polymerase, template, and one oligonucleotide but in the presence of one oligonucleotide; 4, in the absence of primers and template, but in the presence of Taq Gold polymerase; 5, in the absence of template, but in the presence of both primers and polymerase; 6, as in case 3, except in the presence of the alternative primer of the primer combination; 7, in which specific amplicon is produced; and 8, in which no amplicon is expected. Conditions 1, 2, and 6 show the effect of CABS in reducing the background fluorescence caused by the single-strand oligonucleotides. The reaction with CABS in conditions 5 and 8 shows the residual effect caused by primer/dimers, in the case of 8 with additional template DNA.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Alkaline-mediated differential interaction (AMDI): A simple automatable single-nucleotide polymorphism assay

    doi: 10.1073/pnas.041619998

    Figure Lengend Snippet: Effect of individual constituents of the PCR on background fluorescence in the presence or absence of CABS. Each column represents the result of three PCRs performed: 1, in the absence of Taq Gold polymerase, but in the presence of oligonucleotides and template; 2, in the absence of Taq Gold polymerase and template, but in the presence of oligonucleotides; 3, in the absence of Taq Gold polymerase, template, and one oligonucleotide but in the presence of one oligonucleotide; 4, in the absence of primers and template, but in the presence of Taq Gold polymerase; 5, in the absence of template, but in the presence of both primers and polymerase; 6, as in case 3, except in the presence of the alternative primer of the primer combination; 7, in which specific amplicon is produced; and 8, in which no amplicon is expected. Conditions 1, 2, and 6 show the effect of CABS in reducing the background fluorescence caused by the single-strand oligonucleotides. The reaction with CABS in conditions 5 and 8 shows the residual effect caused by primer/dimers, in the case of 8 with additional template DNA.

    Article Snippet: The reaction mixture consisted of 67 mM Tris⋅HCl (pH 8.4) at 25°C, 16.6 mM ammonium sulfate, 2 mM magnesium chloride, 0.01% (vol/vol) Tween 20, 200 μM of each dNTP, 1 μM of each primer, and 0.5 units Hotstart Taq DNA polymerase (Qiagen) or AmpliTaq Gold (Perkin–Elmer).

    Techniques: Polymerase Chain Reaction, Fluorescence, Amplification, Produced

    TALEN-induced mutations in the Platynereis estrogen receptor . (A) Schematic of the er locus showing target exons 2 and 3 with TALEN target sites (yellow). Blue arrows, primer positions; red double-ended arrow, region of sequence deleted in E; green, DNA-binding domain. Primer combinations used for screening are shown above in B–E. (B–E) PCR, undigested PCR product; NI, non-injected. (B) Restriction digest screening of larvae injected with er Ex3_L2/R2 TALENs (mRNA concentration: 267 ng/µl/TALEN mRNA). Arrowhead indicates uncut PCR product following AflI II digestion (asterisk). (C) Mutation evidence at exon 2 site: uncut band adult worm +3 vs. fully digested product from mutation-negative (−) TALEN-injected worm. (D) Adult worms er+31 and er+37 with mutations at exon 3 site. (E) Deletions (red arrow) detected in larvae and adult worms resulting from simultaneous cleavage at exons 2 and 3 using 300 ng/µl/TALEN mRNA: deletion positive (+); deletion negative (−). Please note different primer pairs used for larval vs. adult samples. (F) Mutant sequences obtained from digest screening for exons 1, 2, and long deletions. Numbers in brackets indicate the sample or worm from which the sequence was obtained; all other sequences are from injected larvae shown in B. Length of mutations are indicated by ∆ with “−” indicating deletions and “+” indicating insertions. Restriction site is shown in boldface type; asterisks indicate frameshift mutations. Shading key: yellow, TALEN binding sites; gray, spacer; blue, nucleotides differing from wild type; green, inserted nucleotides.

    Journal: Genetics

    Article Title: TALENs Mediate Efficient and Heritable Mutation of Endogenous Genes in the Marine Annelid Platynereis dumerilii

    doi: 10.1534/genetics.113.161091

    Figure Lengend Snippet: TALEN-induced mutations in the Platynereis estrogen receptor . (A) Schematic of the er locus showing target exons 2 and 3 with TALEN target sites (yellow). Blue arrows, primer positions; red double-ended arrow, region of sequence deleted in E; green, DNA-binding domain. Primer combinations used for screening are shown above in B–E. (B–E) PCR, undigested PCR product; NI, non-injected. (B) Restriction digest screening of larvae injected with er Ex3_L2/R2 TALENs (mRNA concentration: 267 ng/µl/TALEN mRNA). Arrowhead indicates uncut PCR product following AflI II digestion (asterisk). (C) Mutation evidence at exon 2 site: uncut band adult worm +3 vs. fully digested product from mutation-negative (−) TALEN-injected worm. (D) Adult worms er+31 and er+37 with mutations at exon 3 site. (E) Deletions (red arrow) detected in larvae and adult worms resulting from simultaneous cleavage at exons 2 and 3 using 300 ng/µl/TALEN mRNA: deletion positive (+); deletion negative (−). Please note different primer pairs used for larval vs. adult samples. (F) Mutant sequences obtained from digest screening for exons 1, 2, and long deletions. Numbers in brackets indicate the sample or worm from which the sequence was obtained; all other sequences are from injected larvae shown in B. Length of mutations are indicated by ∆ with “−” indicating deletions and “+” indicating insertions. Restriction site is shown in boldface type; asterisks indicate frameshift mutations. Shading key: yellow, TALEN binding sites; gray, spacer; blue, nucleotides differing from wild type; green, inserted nucleotides.

    Article Snippet: PCR and restriction digest screening assays PCR reaction mixes contained DNA polymerase [either HotStar Taq Plus (Qiagen) or Phusion Polymerase (Fermentas)], 1.5–3 mM MgCl2 , 400 μM dNTPs, 200 μM of each primer, 1× reaction buffer (according to the enzyme used), 1–2 µl of DNA template in final volume of 25 or 50 µl.

    Techniques: Sequencing, Binding Assay, Polymerase Chain Reaction, Injection, TALENs, Concentration Assay, Mutagenesis