hotfire dna polymerase  (Solis BioDyne)


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    Name:
    HOT TERMIPol DNA Polymerase
    Description:
    Thermostable hot start DNA polymerase for highly efficient incorporation of unconventional nucleotides
    Catalog Number:
    01-06-00500
    Price:
    None
    Category:
    DNA Polymerase
    Applications:
    MALDI-TOF, massarray, fluorescent labelling, HOT-start
    Size:
    500 U | 100 µl
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    Structured Review

    Solis BioDyne hotfire dna polymerase
    HOT TERMIPol DNA Polymerase
    Thermostable hot start DNA polymerase for highly efficient incorporation of unconventional nucleotides
    https://www.bioz.com/result/hotfire dna polymerase/product/Solis BioDyne
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hotfire dna polymerase - by Bioz Stars, 2021-09
    92/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Temporal Development of the Infant Gut Microbiota in Immunoglobulin E-Sensitized and Nonsensitized Children Determined by the GA-Map Infant Array ▿Temporal Development of the Infant Gut Microbiota in Immunoglobulin E-Sensitized and Nonsensitized Children Determined by the GA-Map Infant Array ▿ †
    Article Snippet: .. Based on the quantification from the gel images, the PCR products were diluted to equal concentrations of 50 ng/μl/sample, and approximately 100 ng template was used in the following labeling reaction mixture: in a total reaction volume of 10 μl, 2.5 U Hot TermiPol (Solis Biodyne), 1× buffer C (Solis Biodyne), 4 mM MgCl2 (Solis Biodyne), 0.4 μM ddCTP-TAMRA (6-carboxytetramethylrhodamine) (Jena Bioscience, Jena, Germany) and 2.9 μM probe set 3 ( ). ..

    Labeling:

    Article Title: Temporal Development of the Infant Gut Microbiota in Immunoglobulin E-Sensitized and Nonsensitized Children Determined by the GA-Map Infant Array ▿Temporal Development of the Infant Gut Microbiota in Immunoglobulin E-Sensitized and Nonsensitized Children Determined by the GA-Map Infant Array ▿ †
    Article Snippet: .. Based on the quantification from the gel images, the PCR products were diluted to equal concentrations of 50 ng/μl/sample, and approximately 100 ng template was used in the following labeling reaction mixture: in a total reaction volume of 10 μl, 2.5 U Hot TermiPol (Solis Biodyne), 1× buffer C (Solis Biodyne), 4 mM MgCl2 (Solis Biodyne), 0.4 μM ddCTP-TAMRA (6-carboxytetramethylrhodamine) (Jena Bioscience, Jena, Germany) and 2.9 μM probe set 3 ( ). ..

    Concentration Assay:

    Article Title: Liquid array diagnostics: a novel method for rapid detection of microbial communities in single-tube multiplex reactions.
    Article Snippet: .. Single nucleotide extension of the LPsA total reaction volume of 15 μl comprising 5 μl Exo-SAP-treated template (or water as ‘no template’ control), LPs at a final concentration of 0.1 μM, 0.8 μM ddCTP-ATTO612Q (Jena Biosciences, Germany), 20 μM ddTTP, 1 mM MgCl2, 1XC buffer and 0.25 U HOT TERMIPOL® DNA Polymerase (all from Solis Biodyne, Estonia) was prepared. ..

    Methylation:

    Article Title: Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancer.
    Article Snippet: .. SIRPH-analysis for methylation testing of individual CpG positions To investigate differential methylation at gene-body sites we used a single nucleotide primer extension (SNuPE) assay combined with reverse phase high performance liquid chromatography (IP RP HPLC) as described previously.17 Fifty nanograms of bisulphite DNA were used as a template in a 30 μl reaction volume in the presence of 3 mM Tris–HCl, pH 8.8, 0.7 mM (NH4)2SO4, 50 mM KCl, 2.5 mM MgCl2, 0.06 mM of each dNTP, 3 U HotFire DNA polymerase (Solis BioDyne, Tartu, Estonia) and 1 μM primers. ..

    Snupe Assay:

    Article Title: Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancer.
    Article Snippet: .. SIRPH-analysis for methylation testing of individual CpG positions To investigate differential methylation at gene-body sites we used a single nucleotide primer extension (SNuPE) assay combined with reverse phase high performance liquid chromatography (IP RP HPLC) as described previously.17 Fifty nanograms of bisulphite DNA were used as a template in a 30 μl reaction volume in the presence of 3 mM Tris–HCl, pH 8.8, 0.7 mM (NH4)2SO4, 50 mM KCl, 2.5 mM MgCl2, 0.06 mM of each dNTP, 3 U HotFire DNA polymerase (Solis BioDyne, Tartu, Estonia) and 1 μM primers. ..

    High Performance Liquid Chromatography:

    Article Title: Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancer.
    Article Snippet: .. SIRPH-analysis for methylation testing of individual CpG positions To investigate differential methylation at gene-body sites we used a single nucleotide primer extension (SNuPE) assay combined with reverse phase high performance liquid chromatography (IP RP HPLC) as described previously.17 Fifty nanograms of bisulphite DNA were used as a template in a 30 μl reaction volume in the presence of 3 mM Tris–HCl, pH 8.8, 0.7 mM (NH4)2SO4, 50 mM KCl, 2.5 mM MgCl2, 0.06 mM of each dNTP, 3 U HotFire DNA polymerase (Solis BioDyne, Tartu, Estonia) and 1 μM primers. ..

    Article Title: A fast, cost-efficient and sensitive approach for KRAS mutation detection using multiplexed primer extension with IP/RP-HPLC separation.
    Article Snippet: .. Primer extension and HPLC separation Fifty nanograms of genomic DNA were used as a template in a 30 ll reaction volume in the presence of 3 mM Tris-HCl, pH 8.8, 0.7 mM (NH4)2SO4, 50 mM KCL, 2.5 mM MgCl2, 0.06 mM of each dNTP, 3 U HotFire DNA polymerase (Solis BioDyne, Tartu, Estonia) and 1 lM primers. ..

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  • 92
    Solis BioDyne hotfire dna polymerase
    Hotfire Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hotfire dna polymerase/product/Solis BioDyne
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hotfire dna polymerase - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

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