pcr  (Solis BioDyne)


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    Name:
    HOT FIREPol DNA Polymerase
    Description:
    A chemically modified hot start version of the thermostable Taq DNA polymerase FIREPol This enzyme is activated only by pre incubation at 95°C preventing any unspecific polymerase activity at lower temperatures during reaction set up Increased specificity and sensitivity Reduced primer dimer formation Reaction set up and shipment without ice
    Catalog Number:
    01-02-00500
    Price:
    None
    Category:
    Endpoint PCR
    Applications:
    PCR, qPCR, HOT-start, cloning, sanger sequencing
    Size:
    500 U | 100 µl
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    Structured Review

    Solis BioDyne pcr
    HOT FIREPol DNA Polymerase
    A chemically modified hot start version of the thermostable Taq DNA polymerase FIREPol This enzyme is activated only by pre incubation at 95°C preventing any unspecific polymerase activity at lower temperatures during reaction set up Increased specificity and sensitivity Reduced primer dimer formation Reaction set up and shipment without ice
    https://www.bioz.com/result/pcr/product/Solis BioDyne
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2021-09
    99/100 stars

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    Related Articles

    Quantitative RT-PCR:

    Article Title: Expression of Enzymes Associated with Prostaglandin Synthesis in Equine Conceptuses
    Article Snippet: .. The qRT-PCR was done in 20 µL reactions including 20 ng cDNA, 0.2 mM of each dNTP, 3 mM MgCl2 , 1× buffer B2 (Solis BioDyne, Tartu, Estonia), 300 nM of each primer, 200 nM probe, 50 nM ROX reference dye (Biotium, Hayward, CA, USA) and 1 unit of HOT FIREPol DNA polymerase (Solis BioDyne). ..

    Multiplex Assay:

    Article Title: Epigenetic Changes in Equine Embryos after Short-Term Storage at Different Temperatures
    Article Snippet: .. The multiplex PCR was performed in 25 µL reaction volumes including 200 µM of each dNTP, 1 × buffer B2 (Solis BioDyne, Tartu, Estonia), 3 mM MgCl2 , 120 nM of each outer primer, 1.2 units HOT FIREPol DNA polymerase (Solis BioDyne, Tartu, Estonia) and 2 µL bisufite-treated DNA. ..

    Polymerase Chain Reaction:

    Article Title: Epigenetic Changes in Equine Embryos after Short-Term Storage at Different Temperatures
    Article Snippet: .. The multiplex PCR was performed in 25 µL reaction volumes including 200 µM of each dNTP, 1 × buffer B2 (Solis BioDyne, Tartu, Estonia), 3 mM MgCl2 , 120 nM of each outer primer, 1.2 units HOT FIREPol DNA polymerase (Solis BioDyne, Tartu, Estonia) and 2 µL bisufite-treated DNA. ..

    Article Title: The Foster method: Rapid and non-invasive detection of clinically relevant American Foulbrood disease levels using eDNA sampling and a dual-target qPCR assay, with its potential for other hive pathogens
    Article Snippet: .. Conventional PCRs used the same 2 μL each DNA dilution in a 20 μL PCR using HOT FIREPol polymerase, ready to load (Solis BioDyne, Tartu, Estonia) and 0.3 μM each primer. ..

    Article Title: Single nucleotide polymorphism rs5029937 in TNFAIP3 gene is correlated with risk of rheumatoid arthritis
    Article Snippet: .. HRM was performed using HOT FIREPol EvaGreen HRM Mix (no ROX) HRM PCR kit which contains HOT FIREPol® DNA Polymerase, 5x EvaGreen® HRM buffer, 12.5 mM MgCl2, dNTPs, Bovine serum albumin (BSA), and EvaGreen dye (Solis BioDyne Estonia). ..

    Transfection:

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib
    Article Snippet: .. Genomic DNA was extracted from cells 24 h after transfection and the genomic region containing the CRISPR target site in the TRIB3 gene was amplified with HOT FIREPol DNA Polymerase (Solis BioDyne, Tartu, Estonia) using primers #1050 and #1051 ( ). ..

    CRISPR:

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib
    Article Snippet: .. Genomic DNA was extracted from cells 24 h after transfection and the genomic region containing the CRISPR target site in the TRIB3 gene was amplified with HOT FIREPol DNA Polymerase (Solis BioDyne, Tartu, Estonia) using primers #1050 and #1051 ( ). ..

    Amplification:

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib
    Article Snippet: .. Genomic DNA was extracted from cells 24 h after transfection and the genomic region containing the CRISPR target site in the TRIB3 gene was amplified with HOT FIREPol DNA Polymerase (Solis BioDyne, Tartu, Estonia) using primers #1050 and #1051 ( ). ..

    Generated:

    Article Title: The Role of Small Molecules and Their Effect on the Molecular Mechanisms of Early Retinal Organoid Development
    Article Snippet: .. Total RNA was extracted from 5 to 6 organoids per sample using the Qiagen RNeasy Mini kit with a DNase step according to the manufacturer’s instructions. cDNA was generated using Superscript III (Invitrogen, Waltham, MA, USA), and sq-PCR was performed with HOT FIREpol DNA Polymerase (Solis Biodyne, Tartu, Estonia). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib
    Article Snippet: .. XBP1 splicing was detected by RT-PCR using HOT FIREPol DNA Polymerase (Solis BioDyne) and primers that span the IRE1-mediated splicing event on the XBP1 mRNA ( ). ..

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  • 99
    Solis BioDyne pcr
    Pcr, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr/product/Solis BioDyne
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

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