Journal: Scientific Reports
Article Title: The commensal infant gut meta-mobilome as a potential reservoir for persistent multidrug resistance integrons
Figure Lengend Snippet: Integrons detected by long-range PCR. ( a ) 1.5 kb partially sequenced integron by long-range PCR product. ( b ) 1.1 kb partially sequenced integron by long-range PCR. Cylindrical boxes show individual genes that are size dependent, i.e. larger box is longer gene; dotted arrows indicate the direction of transcription; and gradient blue color the end of the acquired sequence. Gene and structural features: attI , primary recombination site; dfrA17 and dfrA12 , dihydrofolate reductase; attC , recombination site; aadA5 and aadA2 , aminoglycoside adenylyltransferase; and orfF , hypothetical protein.
Article Snippet: Each PCR reaction (25 μl) contained 1× HOT FIREPol PCR mix (Solis BioDyne, Estonia); 200 nM forward and reverse primers; one μl of sample DNA and water.
Techniques: Polymerase Chain Reaction, Sequencing