hot start taq polymerase  (Thermo Fisher)


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  • 93
    Name:
    Maxma Hot Start Taq DNA Polymerase
    Description:

    Catalog Number:
    ep0602
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    None
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    Structured Review

    Thermo Fisher hot start taq polymerase

    https://www.bioz.com/result/hot start taq polymerase/product/Thermo Fisher
    Average 93 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    hot start taq polymerase - by Bioz Stars, 2020-07
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Varietal Tracing of Virgin Olive Oils Based on Plastid DNA Variation Profiling
    Article Snippet: .. Briefly, the PCR mixtures contained: i) the DNA isolated from either leaves or olive oils; ii) the reaction buffer made of 200 mM Tris-HCl (pH 8.3 at 25°C), 200 mM KCl, 50 mM (NH4 )2 SO4 and 3 mM MgCl2 ; iii) 25 mM of each dNTP; iv) 0.6 U of Hot Start Taq DNA polymerase from Fermentas (part of Thermo Fisher Scientific; Glen Burnie, MD, USA); and v) the PCR primers, including 50 pM of the forward primer fluorescently labeled with either 6-FAM, HEX or NED fluorochrome , and 50 pM of the reverse primer. ..

    Article Title: Inducible nitric oxide synthetase genotype and Helicobacter pylori infection affect gastric cancer risk
    Article Snippet: .. Briefly, for each individual a polymerase chain reaction (PCR) was performed using 2 pmol each of the iNOS-F (5’-TGTAAACCAACTTCCGTGGTG-3’) and iNOS-R (5’-174 GTCTCTGCGGGTCTGAAG-3’) primers, 200 mmol dNTPs, 10 mmol Tris-HCl, pH 8.3, 50 mmol KCl, 1.5 mmol MgCl2 , 0.25 U of Hot start Taq DNA polymerase (Fermentas, Italy) and 50 ng of genomic DNA template in a final reaction volume of 25 μL. .. Restriction digests were performed by incubating 10 μL of PCR product with 2 U of TSP 509I enzyme (New England Biolabs, Ipswich, MA, United States) in a final volume of 15 μL for 15 h at 65 °C.

    Article Title: Diarrheagenic Escherichia coli and Shigella with High Rate of Extended-Spectrum Beta-Lactamase Production: Two Predominant Etiological Agents of Acute Diarrhea in Shiraz, Iran
    Article Snippet: .. PCR was performed in the final volume of 50 μl, including 5 μl PCR buffer (Thermo Scientific; Maxima Hot Start Taq DNA polymerase, EP0602), 2.5 mM of MgCl2 (Thermo Scientific; Maxima Hot Start Taq DNA polymerase, EP0602), 0.4 ng of mixed dNTP (Thermo Scientific; R0192), 15 pmol of each primer (Bioneer, South Korea), 2.5 U of Taq polymerase (Thermo Scientific; Maxima Hot Start Taq DNA polymerase, EP0602), and 2 μl of template. .. The solutions were then subjected to the following cycling condition: 94°C for 5 minutes, 94°C for 30 seconds, 55°C for 30 seconds (for st gene, the optimal annealing was at 50°C), and 72°C for 30 seconds (35 cycles), and a final extension step (72°C for 8 minutes) in a thermal cycler (Applied Biosystem, Veriti).

    Article Title: SNP genotyping on a genome-wide amplified DOP-PCR template
    Article Snippet: .. In the 10 µl PCR mix was 200 µM dNTPs, 1.5 mM MgCl2 , 20 mM Tris–HCl (pH 8.4), 50 mM KCl and 2.5 U hot start Taq DNA polymerase (Invitrogen). .. The cycling procedure was the same touchdown protocol as for the FP-TDI PCR.

    Isolation:

    Article Title: Varietal Tracing of Virgin Olive Oils Based on Plastid DNA Variation Profiling
    Article Snippet: .. Briefly, the PCR mixtures contained: i) the DNA isolated from either leaves or olive oils; ii) the reaction buffer made of 200 mM Tris-HCl (pH 8.3 at 25°C), 200 mM KCl, 50 mM (NH4 )2 SO4 and 3 mM MgCl2 ; iii) 25 mM of each dNTP; iv) 0.6 U of Hot Start Taq DNA polymerase from Fermentas (part of Thermo Fisher Scientific; Glen Burnie, MD, USA); and v) the PCR primers, including 50 pM of the forward primer fluorescently labeled with either 6-FAM, HEX or NED fluorochrome , and 50 pM of the reverse primer. ..

    Hot Start PCR:

    Article Title: LOC134466 methylation promotes oncogenesis of endometrial carcinoma through LOC134466/hsa-miR-196a-5p/TAC1 axis
    Article Snippet: .. Meanwhile, we performed Hot-start PCR at an annealing temperature of 60 °C using hot-start Taq DNA polymerase (Thermo Fisher Scientific). .. The isolation of RNA and quantitative real time PCR We used TRIzol reagent (Invitrogen, Carlsbad, USA) to extract the total RNA endometrial carcinoma tissues and corresponding healthy tissues.

    Article Title: MMP2 is associated with glioma malignancy and patient outcome
    Article Snippet: .. The reaction was performed in 15 µL total volume, consisting of: 7.5 µL Hot Start PCR Master Mix with Hot start Taq DNA polymerase (Thermo Fisher Scientific), 4.5 µL nuclease-free water (Thermo Fisher Scientific), 1 µL (10 pmol/µL) of each primer, specific to methylated/unmethylated promoter (Metabion International) and ~20 ng of bisulfite-treated DNA as a template. .. Primers sequences for methylated MMP2 sequence were 5’-GGACGTTAAGGGTTTAGAGC-3’ (sense), 5’-CAATACACGACCTCGTCAC-3’ (antisense), for unmethylated-5’-GGATGTTAAGGGTTTAGAGT-3’ (sense), 5’-CAATACACAACCTCATCAC-3’ (antisense).

    Labeling:

    Article Title: Varietal Tracing of Virgin Olive Oils Based on Plastid DNA Variation Profiling
    Article Snippet: .. Briefly, the PCR mixtures contained: i) the DNA isolated from either leaves or olive oils; ii) the reaction buffer made of 200 mM Tris-HCl (pH 8.3 at 25°C), 200 mM KCl, 50 mM (NH4 )2 SO4 and 3 mM MgCl2 ; iii) 25 mM of each dNTP; iv) 0.6 U of Hot Start Taq DNA polymerase from Fermentas (part of Thermo Fisher Scientific; Glen Burnie, MD, USA); and v) the PCR primers, including 50 pM of the forward primer fluorescently labeled with either 6-FAM, HEX or NED fluorochrome , and 50 pM of the reverse primer. ..

    Methylation:

    Article Title: MMP2 is associated with glioma malignancy and patient outcome
    Article Snippet: .. The reaction was performed in 15 µL total volume, consisting of: 7.5 µL Hot Start PCR Master Mix with Hot start Taq DNA polymerase (Thermo Fisher Scientific), 4.5 µL nuclease-free water (Thermo Fisher Scientific), 1 µL (10 pmol/µL) of each primer, specific to methylated/unmethylated promoter (Metabion International) and ~20 ng of bisulfite-treated DNA as a template. .. Primers sequences for methylated MMP2 sequence were 5’-GGACGTTAAGGGTTTAGAGC-3’ (sense), 5’-CAATACACGACCTCGTCAC-3’ (antisense), for unmethylated-5’-GGATGTTAAGGGTTTAGAGT-3’ (sense), 5’-CAATACACAACCTCATCAC-3’ (antisense).

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  • 96
    Thermo Fisher maxima hot start taq polymerase
    Comparison of different concentrations of <t>Taq</t> -polymerase in PCR with Amplifluor-like <t>SNP</t> markers for ‘trouble-shooting’ of allele discrimination. The identicial PCR protocol was applied with the same DNA samples from a wheat collection from Kazakhstan and the same marker W58, using 0.5 units ( a ) or 0.1 units ( b ) of Maxima Hot-start Taq -polymerase (ThermoFisher, USA). X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals, respectively, as determined by the qPCR instrument
    Maxima Hot Start Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maxima hot start taq polymerase/product/Thermo Fisher
    Average 96 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    maxima hot start taq polymerase - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    92
    Thermo Fisher platinum ii taq hot start dna polymerase
    Effect of DNase I treatment on <t>Taq</t> <t>DNA</t> polymerase-mediated RT-qPCR assay. Taq DNA polymerase purchased from NEB was used to operate CDC SARS-CoV-2 N1, N2, and N3 TaqMan RT-qPCR assays using SARS-CoV-2 viral genomic RNA (panels A-C) or N gene armored RNA (panels D-F) treated with DNase I. Amplification curves shown in panels A-C resulted from 6000 (black traces), 600 (red traces), 60 (blue traces), 6 (pink traces), and 0 (gray traces) copies of SARS-CoV-2 genomic RNA. Amplification curves in panels D-F resulted from 30,000 (black traces), 3,000 (red traces), 300 (blue traces), 30 (pink traces) and 0 (gray traces) copies of N gene armored RNA. Representative Ct values for RT-qPCR amplification of indicated copies of untreated and DNase I treated SARS-CoV-2 genomic RNA and N gene armored RNA are tabulated.
    Platinum Ii Taq Hot Start Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum ii taq hot start dna polymerase/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    platinum ii taq hot start dna polymerase - by Bioz Stars, 2020-07
    92/100 stars
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    99
    Thermo Fisher platinum taq dna polymerase high fidelity
    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by <t>DNA</t> sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the <t>Taq</t> DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.
    Platinum Taq Dna Polymerase High Fidelity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 894 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/platinum taq dna polymerase high fidelity/product/Thermo Fisher
    Average 99 stars, based on 894 article reviews
    Price from $9.99 to $1999.99
    platinum taq dna polymerase high fidelity - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of different concentrations of Taq -polymerase in PCR with Amplifluor-like SNP markers for ‘trouble-shooting’ of allele discrimination. The identicial PCR protocol was applied with the same DNA samples from a wheat collection from Kazakhstan and the same marker W58, using 0.5 units ( a ) or 0.1 units ( b ) of Maxima Hot-start Taq -polymerase (ThermoFisher, USA). X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals, respectively, as determined by the qPCR instrument

    Journal: BMC Plant Biology

    Article Title: Advantages of Amplifluor-like SNP markers over KASP in plant genotyping

    doi: 10.1186/s12870-017-1197-x

    Figure Lengend Snippet: Comparison of different concentrations of Taq -polymerase in PCR with Amplifluor-like SNP markers for ‘trouble-shooting’ of allele discrimination. The identicial PCR protocol was applied with the same DNA samples from a wheat collection from Kazakhstan and the same marker W58, using 0.5 units ( a ) or 0.1 units ( b ) of Maxima Hot-start Taq -polymerase (ThermoFisher, USA). X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals, respectively, as determined by the qPCR instrument

    Article Snippet: Figure shows our results using the same bread wheat collection genotypes and the same SNP primer W58, only with five-fold less Maxima Hot-Start Taq -polymerase (0.1 enzyme units per 10 μl total PCR reaction).

    Techniques: Polymerase Chain Reaction, Marker, Amplification, Fluorescence, Real-time Polymerase Chain Reaction

    Results of ‘RNA-induced DNA replication interference’ assays. a Effects of 27nt-RNAs using Pfu or Taq polymerases after end-point-PCR and agarose gel electropho resis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana (reviewed in [ 38 ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted

    Journal: Epigenetics & Chromatin

    Article Title: 27nt-RNAs guide histone variant deposition via ‘RNA-induced DNA replication interference’ and thus transmit parental genome partitioning in Stylonychia

    doi: 10.1186/s13072-018-0201-5

    Figure Lengend Snippet: Results of ‘RNA-induced DNA replication interference’ assays. a Effects of 27nt-RNAs using Pfu or Taq polymerases after end-point-PCR and agarose gel electropho resis (top) or after qPCR (bottom). b Effects of 27nt-RNAs alone or in combination with PIWI1 on linear DNA amplification in a Klenow reaction were assayed via qPCR. c Hypothetical models on sequence-specific targeting through Argonaute/PIWI-RNA complexes (blue: IES, red: MDS, yellow: PIWI1, green: 27nt-RNA, orange: tethered transcript [ c1 only]): c1 According to the ‚nascent transcript model, 27nt-RNA/PIWI1 complexes could target tethered IES-originating transcripts, which would be reminiscent of observations made in divergent eukaryotes, such as S. pombe , C. elegans and A. thaliana (reviewed in [ 38 ]). Alternatively, we assumed that 27nt-RNA/PIWI1 complexes could interact with dsDNA ( c2 ) or via base-pairing with ssDNA, possibly when it occurs in a replication bubble ( c3 ). d Mapping of mRNA reads purified 20 h PC on micronuclear model genes reveals that IES (red bars) are sharply omitted

    Article Snippet: End-point PCR was carried out on a VWR Doppio PCR device using Maxima Hot Start Taq Polymerase (ThermoFisher) or Pfu DNA Polymerase (ThermoFisher).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction, Amplification, Sequencing, Purification

    Effect of DNase I treatment on Taq DNA polymerase-mediated RT-qPCR assay. Taq DNA polymerase purchased from NEB was used to operate CDC SARS-CoV-2 N1, N2, and N3 TaqMan RT-qPCR assays using SARS-CoV-2 viral genomic RNA (panels A-C) or N gene armored RNA (panels D-F) treated with DNase I. Amplification curves shown in panels A-C resulted from 6000 (black traces), 600 (red traces), 60 (blue traces), 6 (pink traces), and 0 (gray traces) copies of SARS-CoV-2 genomic RNA. Amplification curves in panels D-F resulted from 30,000 (black traces), 3,000 (red traces), 300 (blue traces), 30 (pink traces) and 0 (gray traces) copies of N gene armored RNA. Representative Ct values for RT-qPCR amplification of indicated copies of untreated and DNase I treated SARS-CoV-2 genomic RNA and N gene armored RNA are tabulated.

    Journal: bioRxiv

    Article Title: One enzyme reverse transcription qPCR using Taq DNA polymerase

    doi: 10.1101/2020.05.27.120238

    Figure Lengend Snippet: Effect of DNase I treatment on Taq DNA polymerase-mediated RT-qPCR assay. Taq DNA polymerase purchased from NEB was used to operate CDC SARS-CoV-2 N1, N2, and N3 TaqMan RT-qPCR assays using SARS-CoV-2 viral genomic RNA (panels A-C) or N gene armored RNA (panels D-F) treated with DNase I. Amplification curves shown in panels A-C resulted from 6000 (black traces), 600 (red traces), 60 (blue traces), 6 (pink traces), and 0 (gray traces) copies of SARS-CoV-2 genomic RNA. Amplification curves in panels D-F resulted from 30,000 (black traces), 3,000 (red traces), 300 (blue traces), 30 (pink traces) and 0 (gray traces) copies of N gene armored RNA. Representative Ct values for RT-qPCR amplification of indicated copies of untreated and DNase I treated SARS-CoV-2 genomic RNA and N gene armored RNA are tabulated.

    Article Snippet: The RT-qPCR ability was not restricted to the NEB Taq DNA polymerase.

    Techniques: Quantitative RT-PCR, Amplification

    SARS-CoV-2 N1 TaqMan RT-qPCR assays performed using NEB Taq DNA polymerase and N gene armored RNA in indicated buffers. Buffer compositions are detailed in Table 2 . Amplification curves resulting from 3 × 10 5 (black traces), 3 × 10 4 (red traces), 3 × 10 3 (blue traces), 3 × 10 2 (pink traces), 30 (green traces), and 0 (gray) copies of SARS-CoV-2 N gene armored RNA are depicted.

    Journal: bioRxiv

    Article Title: One enzyme reverse transcription qPCR using Taq DNA polymerase

    doi: 10.1101/2020.05.27.120238

    Figure Lengend Snippet: SARS-CoV-2 N1 TaqMan RT-qPCR assays performed using NEB Taq DNA polymerase and N gene armored RNA in indicated buffers. Buffer compositions are detailed in Table 2 . Amplification curves resulting from 3 × 10 5 (black traces), 3 × 10 4 (red traces), 3 × 10 3 (blue traces), 3 × 10 2 (pink traces), 30 (green traces), and 0 (gray) copies of SARS-CoV-2 N gene armored RNA are depicted.

    Article Snippet: The RT-qPCR ability was not restricted to the NEB Taq DNA polymerase.

    Techniques: Quantitative RT-PCR, Amplification

    TaqMan RT-qPCR analysis of SARS-CoV-2 viral genomic RNA and RNaseP armored RNA using Taq DNA polymerase-based one-enzyme assays. CDC SARS-CoV-2 N gene assays, N1, N2, and N3, and RNaseP assay were performed using Taq DNA polymerase from either NEB (panels A-H) or Thermo Fisher (panels I-P). Assays were performed either using the companion commercial buffer (panels A-D and panels I-L) or using Gen 6 A buffer (panels E-H and panels M-P). Amplification curves from 6000 (black traces), 600 (red traces), 60 (blue traces), 6 (pink traces), and 0 (gray traces) copies of viral genomic RNA are depicted in panels A-C, E-G, I-K, and M-O. Amplification curves from 3 × 10 5 (black traces), 3 × 10 4 (red traces), 3 × 10 3 (blue traces), 3 × 10 2 (pink traces) and 0 (gray traces) copies of armored RNaseP RNA are depicted in panes D, H, L, and P.

    Journal: bioRxiv

    Article Title: One enzyme reverse transcription qPCR using Taq DNA polymerase

    doi: 10.1101/2020.05.27.120238

    Figure Lengend Snippet: TaqMan RT-qPCR analysis of SARS-CoV-2 viral genomic RNA and RNaseP armored RNA using Taq DNA polymerase-based one-enzyme assays. CDC SARS-CoV-2 N gene assays, N1, N2, and N3, and RNaseP assay were performed using Taq DNA polymerase from either NEB (panels A-H) or Thermo Fisher (panels I-P). Assays were performed either using the companion commercial buffer (panels A-D and panels I-L) or using Gen 6 A buffer (panels E-H and panels M-P). Amplification curves from 6000 (black traces), 600 (red traces), 60 (blue traces), 6 (pink traces), and 0 (gray traces) copies of viral genomic RNA are depicted in panels A-C, E-G, I-K, and M-O. Amplification curves from 3 × 10 5 (black traces), 3 × 10 4 (red traces), 3 × 10 3 (blue traces), 3 × 10 2 (pink traces) and 0 (gray traces) copies of armored RNaseP RNA are depicted in panes D, H, L, and P.

    Article Snippet: The RT-qPCR ability was not restricted to the NEB Taq DNA polymerase.

    Techniques: Quantitative RT-PCR, Amplification

    Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Journal: BioMed Research International

    Article Title: LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity

    doi: 10.1155/2018/5657085

    Figure Lengend Snippet: Determination of the Lra I cleavage site 5′-G/AATTC-3′ of pBluescipt SK+ by DNA sequencing. (a) Sequence of pBluescipt SK+ predigested with Lra I restriction enzyme and (b) with Eco RI obtained using M13R primer. The colour-coded sequence traces are A (green), T (red), C (blue), and G (black). The extra A base was added at the end of the cleaved template by the Taq DNA polymerase used for sequencing due to template-independent terminal nucleotide transferase activity of Taq DNA polymerase.

    Article Snippet: PCR fragments of LraI operon amplified using Platinum™ Taq DNA Polymerase High Fidelity were cloned into pAZIL vector predigested with Sma I.

    Techniques: DNA Sequencing, Sequencing, Activity Assay