hot start taq dna polymerase hot firepol  (Solis BioDyne)


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    Solis BioDyne hot start taq dna polymerase hot firepol
    Hot Start Taq Dna Polymerase Hot Firepol, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    master mix 1 25 u hot start taq dna polymerase solis biodyne estonia  (Solis BioDyne)


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    Solis BioDyne master mix 1 25 u hot start taq dna polymerase solis biodyne estonia
    Master Mix 1 25 U Hot Start Taq Dna Polymerase Solis Biodyne Estonia, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hot start taq dna polymerase  (Solis BioDyne)


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    Solis BioDyne hot start taq dna polymerase
    Hot Start Taq Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hot start taq dna polymerase hot firepol  (Solis BioDyne)


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    Solis BioDyne hot start taq dna polymerase hot firepol
    Hot Start Taq Dna Polymerase Hot Firepol, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot start taq dna polymerase hot firepol/product/Solis BioDyne
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    hot start firepol taq dna polymerase  (Solis BioDyne)


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    Solis BioDyne hot start firepol taq dna polymerase
    <t>DNA</t> preparation and PCR methods evaluated. Three methods were used to obtain DNA from flea larvae, including a soil DNA isolation kit (S-kit), an ammonium acetate precipitation protocol (AmAcet), and a crude flea lysate protocol. Samples from all extraction protocols were used for PCR amplification using either a hot-start <t>FIREPol</t> ® <t>Taq</t> DNA polymerase or the highly inhibitor-resistant Phusion ® HF DNA polymerase.
    Hot Start Firepol Taq Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot start firepol taq dna polymerase/product/Solis BioDyne
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hot start firepol taq dna polymerase - by Bioz Stars, 2024-07
    96/100 stars

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    1) Product Images from "Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted from Soil Samples Containing PCR Inhibitor-Rich Material"

    Article Title: Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted from Soil Samples Containing PCR Inhibitor-Rich Material

    Journal: Insects

    doi: 10.3390/insects14010005

    DNA preparation and PCR methods evaluated. Three methods were used to obtain DNA from flea larvae, including a soil DNA isolation kit (S-kit), an ammonium acetate precipitation protocol (AmAcet), and a crude flea lysate protocol. Samples from all extraction protocols were used for PCR amplification using either a hot-start FIREPol ® Taq DNA polymerase or the highly inhibitor-resistant Phusion ® HF DNA polymerase.
    Figure Legend Snippet: DNA preparation and PCR methods evaluated. Three methods were used to obtain DNA from flea larvae, including a soil DNA isolation kit (S-kit), an ammonium acetate precipitation protocol (AmAcet), and a crude flea lysate protocol. Samples from all extraction protocols were used for PCR amplification using either a hot-start FIREPol ® Taq DNA polymerase or the highly inhibitor-resistant Phusion ® HF DNA polymerase.

    Techniques Used: DNA Extraction, Amplification

    Primer information. Target gene and primer sequences for both forward and reverse primers.
    Figure Legend Snippet: Primer information. Target gene and primer sequences for both forward and reverse primers.

    Techniques Used: Sequencing

    Representative amplification plots for the Tunga penetrans using FIREPol ® Taq ( A ) and Phusion ® ( B ) polymerases. The different DNA preparation methods are color-coded, including pink for AmAcet, blue for S-kit, and black for CL.
    Figure Legend Snippet: Representative amplification plots for the Tunga penetrans using FIREPol ® Taq ( A ) and Phusion ® ( B ) polymerases. The different DNA preparation methods are color-coded, including pink for AmAcet, blue for S-kit, and black for CL.

    Techniques Used: Amplification

    Success rate for different PCRs and  DNA  preparation methods based on 30 replicates.
    Figure Legend Snippet: Success rate for different PCRs and DNA preparation methods based on 30 replicates.

    Techniques Used:

    Comparison of the cycles of quantification (C q value) ( A ) and PCR efficacies ( B ) between different DNA preparation methods and amplification protocols for a Tunga penetrans partial ITS-2 PCR. Template DNA was obtained (i) as a crude lysate (CL) by simple boiling of mechanically cracked larvae, (ii) by proteinase K digestion followed by precipitation with ammonium acetate (AmAcet) or (iii) using a soil DNA isolation kit (S-kit). Amplification was performed as real-time PCR using either the FIREPol ® Taq (Taq) or the Phusion ® (Phu) DNA polymerase protocols. Boxplots show medians with interquartile ranges and whiskers represent 5 and 95% quantiles. Outliers are indicated by dots. The mean of all values is shown as a cross. Paired t -tests for the same template DNA using either Taq or Phusion ® polymerase protocols did not reveal any significant differences. Comparison between different DNA preparation methods using the same amplification protocol were conducted using one-way ANOVAs. Hashtags were used to indicate differences between the DNA preparation methods for the Taq polymerase protocol, while asteriks indicate differences between the preparation methods for the Phusion ® protocol. #, p < 0.05; ***, p < 0.001.
    Figure Legend Snippet: Comparison of the cycles of quantification (C q value) ( A ) and PCR efficacies ( B ) between different DNA preparation methods and amplification protocols for a Tunga penetrans partial ITS-2 PCR. Template DNA was obtained (i) as a crude lysate (CL) by simple boiling of mechanically cracked larvae, (ii) by proteinase K digestion followed by precipitation with ammonium acetate (AmAcet) or (iii) using a soil DNA isolation kit (S-kit). Amplification was performed as real-time PCR using either the FIREPol ® Taq (Taq) or the Phusion ® (Phu) DNA polymerase protocols. Boxplots show medians with interquartile ranges and whiskers represent 5 and 95% quantiles. Outliers are indicated by dots. The mean of all values is shown as a cross. Paired t -tests for the same template DNA using either Taq or Phusion ® polymerase protocols did not reveal any significant differences. Comparison between different DNA preparation methods using the same amplification protocol were conducted using one-way ANOVAs. Hashtags were used to indicate differences between the DNA preparation methods for the Taq polymerase protocol, while asteriks indicate differences between the preparation methods for the Phusion ® protocol. #, p < 0.05; ***, p < 0.001.

    Techniques Used: Amplification, DNA Extraction, Real-time Polymerase Chain Reaction

    Comparison of the cycles of quantification (C q value) ( A ) and PCR efficacies ( B ) between different DNA preparation methods and amplification protocols for a cox2 partial PCR. Template DNA was obtained as a crude lysate (CL) by simple boiling of mechanically cracked larvae and by proteinase K digestion, followed by precipitation with ammonium acetate (AmAcet) or using a soil DNA isolation kit (S-kit). Amplification was performed as a real-time PCR using either the FIREPol ® Taq (Taq) or the Phusion ® (Phu) DNA polymerase protocols. Boxplots show medians with interquartile ranges and whiskers represent 5 and 95% quantiles. Outliers are indicated by dots. The mean of all the values is shown as a cross. Paired t -tests for the same template DNA using either Taq or Phusion ® polymerase protocols did not reveal any significant differences. Comparisons between different DNA preparation methods using the same amplification protocol were conducted using one-way ANOVAs. # is used to indicate differences between the DNA preparation methods for the Taq polymerase protocol, while * indicates differences between the preparation methods for the Phusion ® protocol. ¤ is used to indicate differences between FIREPol ® Taq and Phusion ® in paired analyses. *, #, p < 0.05; ¤¤, **, p < 0.01; ¤¤¤, ***, ###, p < 0.001.
    Figure Legend Snippet: Comparison of the cycles of quantification (C q value) ( A ) and PCR efficacies ( B ) between different DNA preparation methods and amplification protocols for a cox2 partial PCR. Template DNA was obtained as a crude lysate (CL) by simple boiling of mechanically cracked larvae and by proteinase K digestion, followed by precipitation with ammonium acetate (AmAcet) or using a soil DNA isolation kit (S-kit). Amplification was performed as a real-time PCR using either the FIREPol ® Taq (Taq) or the Phusion ® (Phu) DNA polymerase protocols. Boxplots show medians with interquartile ranges and whiskers represent 5 and 95% quantiles. Outliers are indicated by dots. The mean of all the values is shown as a cross. Paired t -tests for the same template DNA using either Taq or Phusion ® polymerase protocols did not reveal any significant differences. Comparisons between different DNA preparation methods using the same amplification protocol were conducted using one-way ANOVAs. # is used to indicate differences between the DNA preparation methods for the Taq polymerase protocol, while * indicates differences between the preparation methods for the Phusion ® protocol. ¤ is used to indicate differences between FIREPol ® Taq and Phusion ® in paired analyses. *, #, p < 0.05; ¤¤, **, p < 0.01; ¤¤¤, ***, ###, p < 0.001.

    Techniques Used: Amplification, DNA Extraction, Real-time Polymerase Chain Reaction

    hot start taq dna polymerase  (Solis BioDyne)


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    hot start taq polymerase  (Solis BioDyne)


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    Solis BioDyne hot start taq polymerase
    Hot Start Taq Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hot start taq polymerase  (Solis BioDyne)


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    Solis BioDyne hot start taq polymerase
    Hot Start Taq Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hot start taq polymerase  (Solis BioDyne)


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    Solis BioDyne hot start taq polymerase
    Hot Start Taq Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hot start taq dna polymerase  (Solis BioDyne)


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    Solis BioDyne hot start taq dna polymerase
    Hot Start Taq Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hot start taq dna polymerase  (Solis BioDyne)


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    Solis BioDyne hot start taq dna polymerase
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    Solis BioDyne hot start taq dna polymerase hot firepol
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    Solis BioDyne hot start firepol taq dna polymerase
    <t>DNA</t> preparation and PCR methods evaluated. Three methods were used to obtain DNA from flea larvae, including a soil DNA isolation kit (S-kit), an ammonium acetate precipitation protocol (AmAcet), and a crude flea lysate protocol. Samples from all extraction protocols were used for PCR amplification using either a hot-start <t>FIREPol</t> ® <t>Taq</t> DNA polymerase or the highly inhibitor-resistant Phusion ® HF DNA polymerase.
    Hot Start Firepol Taq Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot start firepol taq dna polymerase/product/Solis BioDyne
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hot start firepol taq dna polymerase - by Bioz Stars, 2024-07
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    Solis BioDyne hot start taq polymerase
    <t>DNA</t> preparation and PCR methods evaluated. Three methods were used to obtain DNA from flea larvae, including a soil DNA isolation kit (S-kit), an ammonium acetate precipitation protocol (AmAcet), and a crude flea lysate protocol. Samples from all extraction protocols were used for PCR amplification using either a hot-start <t>FIREPol</t> ® <t>Taq</t> DNA polymerase or the highly inhibitor-resistant Phusion ® HF DNA polymerase.
    Hot Start Taq Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot start taq polymerase/product/Solis BioDyne
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hot start taq polymerase - by Bioz Stars, 2024-07
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    DNA preparation and PCR methods evaluated. Three methods were used to obtain DNA from flea larvae, including a soil DNA isolation kit (S-kit), an ammonium acetate precipitation protocol (AmAcet), and a crude flea lysate protocol. Samples from all extraction protocols were used for PCR amplification using either a hot-start FIREPol ® Taq DNA polymerase or the highly inhibitor-resistant Phusion ® HF DNA polymerase.

    Journal: Insects

    Article Title: Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted from Soil Samples Containing PCR Inhibitor-Rich Material

    doi: 10.3390/insects14010005

    Figure Lengend Snippet: DNA preparation and PCR methods evaluated. Three methods were used to obtain DNA from flea larvae, including a soil DNA isolation kit (S-kit), an ammonium acetate precipitation protocol (AmAcet), and a crude flea lysate protocol. Samples from all extraction protocols were used for PCR amplification using either a hot-start FIREPol ® Taq DNA polymerase or the highly inhibitor-resistant Phusion ® HF DNA polymerase.

    Article Snippet: As indicated in , DNA samples obtained using the three DNA preparation protocols were then used for amplification using either a hot-start FIREPol ® Taq DNA polymerase (Solis BioDyne, Tartu, Estonia) or Phusion ® DNA polymerase (New England Biolabs, Ipswich, MA, USA).

    Techniques: DNA Extraction, Amplification

    Primer information. Target gene and primer sequences for both forward and reverse primers.

    Journal: Insects

    Article Title: Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted from Soil Samples Containing PCR Inhibitor-Rich Material

    doi: 10.3390/insects14010005

    Figure Lengend Snippet: Primer information. Target gene and primer sequences for both forward and reverse primers.

    Article Snippet: As indicated in , DNA samples obtained using the three DNA preparation protocols were then used for amplification using either a hot-start FIREPol ® Taq DNA polymerase (Solis BioDyne, Tartu, Estonia) or Phusion ® DNA polymerase (New England Biolabs, Ipswich, MA, USA).

    Techniques: Sequencing

    Representative amplification plots for the Tunga penetrans using FIREPol ® Taq ( A ) and Phusion ® ( B ) polymerases. The different DNA preparation methods are color-coded, including pink for AmAcet, blue for S-kit, and black for CL.

    Journal: Insects

    Article Title: Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted from Soil Samples Containing PCR Inhibitor-Rich Material

    doi: 10.3390/insects14010005

    Figure Lengend Snippet: Representative amplification plots for the Tunga penetrans using FIREPol ® Taq ( A ) and Phusion ® ( B ) polymerases. The different DNA preparation methods are color-coded, including pink for AmAcet, blue for S-kit, and black for CL.

    Article Snippet: As indicated in , DNA samples obtained using the three DNA preparation protocols were then used for amplification using either a hot-start FIREPol ® Taq DNA polymerase (Solis BioDyne, Tartu, Estonia) or Phusion ® DNA polymerase (New England Biolabs, Ipswich, MA, USA).

    Techniques: Amplification

    Success rate for different PCRs and  DNA  preparation methods based on 30 replicates.

    Journal: Insects

    Article Title: Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted from Soil Samples Containing PCR Inhibitor-Rich Material

    doi: 10.3390/insects14010005

    Figure Lengend Snippet: Success rate for different PCRs and DNA preparation methods based on 30 replicates.

    Article Snippet: As indicated in , DNA samples obtained using the three DNA preparation protocols were then used for amplification using either a hot-start FIREPol ® Taq DNA polymerase (Solis BioDyne, Tartu, Estonia) or Phusion ® DNA polymerase (New England Biolabs, Ipswich, MA, USA).

    Techniques:

    Comparison of the cycles of quantification (C q value) ( A ) and PCR efficacies ( B ) between different DNA preparation methods and amplification protocols for a Tunga penetrans partial ITS-2 PCR. Template DNA was obtained (i) as a crude lysate (CL) by simple boiling of mechanically cracked larvae, (ii) by proteinase K digestion followed by precipitation with ammonium acetate (AmAcet) or (iii) using a soil DNA isolation kit (S-kit). Amplification was performed as real-time PCR using either the FIREPol ® Taq (Taq) or the Phusion ® (Phu) DNA polymerase protocols. Boxplots show medians with interquartile ranges and whiskers represent 5 and 95% quantiles. Outliers are indicated by dots. The mean of all values is shown as a cross. Paired t -tests for the same template DNA using either Taq or Phusion ® polymerase protocols did not reveal any significant differences. Comparison between different DNA preparation methods using the same amplification protocol were conducted using one-way ANOVAs. Hashtags were used to indicate differences between the DNA preparation methods for the Taq polymerase protocol, while asteriks indicate differences between the preparation methods for the Phusion ® protocol. #, p < 0.05; ***, p < 0.001.

    Journal: Insects

    Article Title: Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted from Soil Samples Containing PCR Inhibitor-Rich Material

    doi: 10.3390/insects14010005

    Figure Lengend Snippet: Comparison of the cycles of quantification (C q value) ( A ) and PCR efficacies ( B ) between different DNA preparation methods and amplification protocols for a Tunga penetrans partial ITS-2 PCR. Template DNA was obtained (i) as a crude lysate (CL) by simple boiling of mechanically cracked larvae, (ii) by proteinase K digestion followed by precipitation with ammonium acetate (AmAcet) or (iii) using a soil DNA isolation kit (S-kit). Amplification was performed as real-time PCR using either the FIREPol ® Taq (Taq) or the Phusion ® (Phu) DNA polymerase protocols. Boxplots show medians with interquartile ranges and whiskers represent 5 and 95% quantiles. Outliers are indicated by dots. The mean of all values is shown as a cross. Paired t -tests for the same template DNA using either Taq or Phusion ® polymerase protocols did not reveal any significant differences. Comparison between different DNA preparation methods using the same amplification protocol were conducted using one-way ANOVAs. Hashtags were used to indicate differences between the DNA preparation methods for the Taq polymerase protocol, while asteriks indicate differences between the preparation methods for the Phusion ® protocol. #, p < 0.05; ***, p < 0.001.

    Article Snippet: As indicated in , DNA samples obtained using the three DNA preparation protocols were then used for amplification using either a hot-start FIREPol ® Taq DNA polymerase (Solis BioDyne, Tartu, Estonia) or Phusion ® DNA polymerase (New England Biolabs, Ipswich, MA, USA).

    Techniques: Amplification, DNA Extraction, Real-time Polymerase Chain Reaction

    Comparison of the cycles of quantification (C q value) ( A ) and PCR efficacies ( B ) between different DNA preparation methods and amplification protocols for a cox2 partial PCR. Template DNA was obtained as a crude lysate (CL) by simple boiling of mechanically cracked larvae and by proteinase K digestion, followed by precipitation with ammonium acetate (AmAcet) or using a soil DNA isolation kit (S-kit). Amplification was performed as a real-time PCR using either the FIREPol ® Taq (Taq) or the Phusion ® (Phu) DNA polymerase protocols. Boxplots show medians with interquartile ranges and whiskers represent 5 and 95% quantiles. Outliers are indicated by dots. The mean of all the values is shown as a cross. Paired t -tests for the same template DNA using either Taq or Phusion ® polymerase protocols did not reveal any significant differences. Comparisons between different DNA preparation methods using the same amplification protocol were conducted using one-way ANOVAs. # is used to indicate differences between the DNA preparation methods for the Taq polymerase protocol, while * indicates differences between the preparation methods for the Phusion ® protocol. ¤ is used to indicate differences between FIREPol ® Taq and Phusion ® in paired analyses. *, #, p < 0.05; ¤¤, **, p < 0.01; ¤¤¤, ***, ###, p < 0.001.

    Journal: Insects

    Article Title: Cost-Effective PCR-Based Identification of Tunga penetrans (Siphonaptera) Larvae Extracted from Soil Samples Containing PCR Inhibitor-Rich Material

    doi: 10.3390/insects14010005

    Figure Lengend Snippet: Comparison of the cycles of quantification (C q value) ( A ) and PCR efficacies ( B ) between different DNA preparation methods and amplification protocols for a cox2 partial PCR. Template DNA was obtained as a crude lysate (CL) by simple boiling of mechanically cracked larvae and by proteinase K digestion, followed by precipitation with ammonium acetate (AmAcet) or using a soil DNA isolation kit (S-kit). Amplification was performed as a real-time PCR using either the FIREPol ® Taq (Taq) or the Phusion ® (Phu) DNA polymerase protocols. Boxplots show medians with interquartile ranges and whiskers represent 5 and 95% quantiles. Outliers are indicated by dots. The mean of all the values is shown as a cross. Paired t -tests for the same template DNA using either Taq or Phusion ® polymerase protocols did not reveal any significant differences. Comparisons between different DNA preparation methods using the same amplification protocol were conducted using one-way ANOVAs. # is used to indicate differences between the DNA preparation methods for the Taq polymerase protocol, while * indicates differences between the preparation methods for the Phusion ® protocol. ¤ is used to indicate differences between FIREPol ® Taq and Phusion ® in paired analyses. *, #, p < 0.05; ¤¤, **, p < 0.01; ¤¤¤, ***, ###, p < 0.001.

    Article Snippet: As indicated in , DNA samples obtained using the three DNA preparation protocols were then used for amplification using either a hot-start FIREPol ® Taq DNA polymerase (Solis BioDyne, Tartu, Estonia) or Phusion ® DNA polymerase (New England Biolabs, Ipswich, MA, USA).

    Techniques: Amplification, DNA Extraction, Real-time Polymerase Chain Reaction