hot start taq polymerase  (Qiagen)

 
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    Name:
    Taq DNA Polymerase
    Description:
    Qiagen Taq DNA Polymerase 250U 5U L 10 min at 97C 60 min at 94C Half life 2 to 4 kb min at 72C Extension Rate Genomic DNA and cDNA Sample PCR Amplification Exonuclease Enzyme Activity Minimal Optimization Ideal for Standard and Specialized PCR Applications Includes Taq DNA Polymerase 10x PCR Buffer 10x CoralLoad PCR Buffer 5x Q Solution 25mM MgCl2
    Catalog Number:
    201203
    Price:
    134
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    Structured Review

    Qiagen hot start taq polymerase
    Qiagen Taq DNA Polymerase 250U 5U L 10 min at 97C 60 min at 94C Half life 2 to 4 kb min at 72C Extension Rate Genomic DNA and cDNA Sample PCR Amplification Exonuclease Enzyme Activity Minimal Optimization Ideal for Standard and Specialized PCR Applications Includes Taq DNA Polymerase 10x PCR Buffer 10x CoralLoad PCR Buffer 5x Q Solution 25mM MgCl2
    https://www.bioz.com/result/hot start taq polymerase/product/Qiagen
    Average 99 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    hot start taq polymerase - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Multiplexing:

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid
    Article Snippet: .. The multiplex RT-PCR assay was optimized with regular Taq (Bangalore Genei, Bengaluru, India), Hot Start Taq (Fermentas, Vilnus, Lithuania) DNA polymerase and multiplexing kit from Qiagen under the conditions described above with nad5 as an internal control. .. Leaf samples were tested by double-antibody sandwich ELISA (DAS-ELISA) for the targeted viruses ACLSV, ApMV, ASPV, and ASGV using a commercial kit (Bioreba, Reinach, Switzerland).

    Multiplex Assay:

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid
    Article Snippet: .. The multiplex RT-PCR assay was optimized with regular Taq (Bangalore Genei, Bengaluru, India), Hot Start Taq (Fermentas, Vilnus, Lithuania) DNA polymerase and multiplexing kit from Qiagen under the conditions described above with nad5 as an internal control. .. Leaf samples were tested by double-antibody sandwich ELISA (DAS-ELISA) for the targeted viruses ACLSV, ApMV, ASPV, and ASGV using a commercial kit (Bioreba, Reinach, Switzerland).

    Article Title: Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein
    Article Snippet: .. PCR was performed using DNA polymerase from Thermus aquaticus (Taq ) according to the manufacturer's instructions (QIAGEN HotSartTaq DNA polymerase kit and QIAGEN Multiplex PCR kit, QIAGEN). .. PCR thermal cycles used were 15 min at 95°C for DNA denaturation, followed by 35 cycles of 30 s at 94°C, 90 s at 60°C and 90 s at 72°C.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Simultaneous Detection of Major Pome Fruit Viruses and a Viroid
    Article Snippet: .. The multiplex RT-PCR assay was optimized with regular Taq (Bangalore Genei, Bengaluru, India), Hot Start Taq (Fermentas, Vilnus, Lithuania) DNA polymerase and multiplexing kit from Qiagen under the conditions described above with nad5 as an internal control. .. Leaf samples were tested by double-antibody sandwich ELISA (DAS-ELISA) for the targeted viruses ACLSV, ApMV, ASPV, and ASGV using a commercial kit (Bioreba, Reinach, Switzerland).

    Southern Blot:

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication
    Article Snippet: .. To verify the nature of these slowly migrating molecules, TNAs extracted from the samples at 24 and 72 h were incubated with Taq DNA polymerase and analyzed by Southern blot (Fig. B). ..

    Concentration Assay:

    Article Title: Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods
    Article Snippet: .. PCR was carried out in a 25 μl volume with a final concentration of 1 × Buffer, 2 mM MgCl2 , 0.2 mM dNTPs, 0.1 μM each primer, 0.034 U/μl Taq DNA polymerase (Qiagen). ..

    Incubation:

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication
    Article Snippet: .. To verify the nature of these slowly migrating molecules, TNAs extracted from the samples at 24 and 72 h were incubated with Taq DNA polymerase and analyzed by Southern blot (Fig. B). ..

    other:

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication
    Article Snippet: TNAs of wt protoplasts cotransfected with pTOM6 together with pTOM100C4(−) or pTOM100NT were treated with Taq DNA polymerase for increasing times (Fig. A).

    Article Title: Transgenically Expressed T-Rep of Tomato Yellow Leaf Curl Sardinia Virus Acts as a trans-Dominant-Negative Mutant, Inhibiting Viral Transcription and Replication
    Article Snippet: A fraction of viral DNA from pTOM6-pTOM100NT-cotransfected protoplasts also reacted with Taq DNA polymerase, generating ocDNA.

    Polymerase Chain Reaction:

    Article Title: Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein
    Article Snippet: .. PCR was performed using DNA polymerase from Thermus aquaticus (Taq ) according to the manufacturer's instructions (QIAGEN HotSartTaq DNA polymerase kit and QIAGEN Multiplex PCR kit, QIAGEN). .. PCR thermal cycles used were 15 min at 95°C for DNA denaturation, followed by 35 cycles of 30 s at 94°C, 90 s at 60°C and 90 s at 72°C.

    Article Title: Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods
    Article Snippet: .. PCR was carried out in a 25 μl volume with a final concentration of 1 × Buffer, 2 mM MgCl2 , 0.2 mM dNTPs, 0.1 μM each primer, 0.034 U/μl Taq DNA polymerase (Qiagen). ..

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  • 99
    Qiagen hot start taq polymerase
    Hot Start Taq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot start taq polymerase/product/Qiagen
    Average 99 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    hot start taq polymerase - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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