Hot Firepol Multiplex Mix Ready, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso"
Article Title: Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso
Journal: PLoS ONE
Figure Legend Snippet: Sensitivity of the newly described multiplex PCR method compared to the corresponding simplex PCR for each of the targeted bacterial taxon. Every reaction was performed with six samples of corresponding control bacteria at different concentrations. Lane 1: 1 ng/μl, lane 2: 0.5 ng/μl, lane 3: 0.1 ng/μl, lane 4: 0.05 ng/μl and lane 5: 0.01ng/μl, lane 6: water control. a-b: P . fuscovaginae strain UBP735, c-d: B . glumae strain NCPPB 3923, e-f: Sphingomonas strain V1-2, g-h: X . oryzae pv. oryzae strain BAI10, i-j: Pantoea strain ARC10. a, c, e, g, i: simplex PCR with only one primer pair in each case. b, d, f, h, j: multiplex PCR including the five primer pairs.
Techniques Used: Multiplex Assay, Polymerase Chain Reaction
Figure Legend Snippet: Detection of the five bacterial taxa using the newly described multiplex PCR protocol. L: molecular size marker, 100 bp DNA ladder ready to load, Solis Biodyne, Pfs : P . fuscovaginae strain UBP735, Bg : B . glumae strain NCPPB 3923, Sph : Sphingomonas strain V1-2, Xoc : X . oryzae pv. oryzicola strain BAI10, Pan : Pantoea strain ARC10, Mix: Equal amounts of all five DNA samples.
Techniques Used: Multiplex Assay, Polymerase Chain Reaction, Marker