Structured Review

AvesLabs horseradish peroxidase
Horseradish Peroxidase, supplied by AvesLabs, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/horseradish peroxidase/product/AvesLabs
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
horseradish peroxidase - by Bioz Stars, 2021-01
92/100 stars

Images

Related Articles

other:

Article Title: Interaction of cochlin and mechanosensitive channel TREK-1 in trabecular meshwork cells influences the regulation of intraocular pressure
Article Snippet: A secondary antibody conjugated to horseradish peroxidase (goat anti-chicken cat# H-1004, Aves Labs Inc.; goat anti-rabbit cat# ab6721, Abcam, Cambridge, MA) was added and cochlin/TREK-1 proteins were detected using enhanced chemiluminescent substrate (ECL) (cat# 32106, Pierce Thermo Fisher Scientific Inc, Rockford, IL).

Article Title: High Turnover Rates for Hydrogen Sulfide Allow for Rapid Regulation of Its Tissue Concentrations
Article Snippet: Secondary anti-chicken antibodies conjugated with horseradish peroxidase (Aves Labs) were used (1:250,000 dilution) and signals were visualized using the chemiluminescent peroxidase substrate kit (SuperSignal® West Dura (Thermo Scientific)).

Article Title: Cochlin Induced TREK-1 Co-Expression and Annexin A2 Secretion: Role in Trabecular Meshwork Cell Elongation and Motility
Article Snippet: A secondary antibody conjugated to horseradish peroxidase (goat anti-chicken cat# H-1004, Aves Labs Inc.) was added and proteins were detected using enhanced chemiluminescent substrate (cat# 32106, Pierce Thermo Fisher Scientific Inc, Rockford, IL).

Article Title: Cochlin, Intraocular Pressure Regulation and Mechanosensing
Article Snippet: A secondary antibody conjugated to horseradish peroxidase (goat anti-chicken cat# H-1004, Aves Labs Inc.) was added and proteins were detected using enhanced chemiluminescent substrate (cat# 32106, Pierce Thermo Fisher Scientific Inc, Rockford, IL).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    AvesLabs hrp conjugated goat anti chicken igy
    Specific binding of the chicken anti-MIP-133 antiserum. (A) Western blotting. Crude supernatants taken from A. castellanii trophozoites grown in mannose were resolved by SDS-PAGE and transferred to PVDF membranes. Membranes were first incubated with chicken anti-MIP-133 antiserum, followed by rabbit anti-chicken IgG-alkaline phosphatase, as described in Materials and Methods. The membranes were then washed and developed with Nitro Blue Tetrazolium-BCIP stock solution. The arrow indicates the 133-kDa band. (B) ELISA. Ninety-six-well plates were coated with 50 μg of MIP-133 and allowed to dry. After drying, wells were blocked and incubated with a 1:50, 1:75, or 1:100 dilution of either the chicken anti-MIP-133 antiserum or preimmune chicken antiserum. Wells were then washed and incubated with goat anti-chicken <t>IgY-HRP.</t> ELISA plates were developed and read at an OD at 405 nm. Bars and error bars represent the means ± SE of results from triplicate experiments. ***, significantly different from values obtained with preimmune serum at the same dilutions ( P
    Hrp Conjugated Goat Anti Chicken Igy, supplied by AvesLabs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti chicken igy/product/AvesLabs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti chicken igy - by Bioz Stars, 2021-01
    88/100 stars
      Buy from Supplier

    85
    AvesLabs horseradish peroxidase linked goat anti chicken igy
    Western blot analysis and immunohistochemical localization of Acanthamoeba <t>MBP.</t> (A) Affinity-purified amoeba MBP was electrophoresed under native conditions. After electrophoresis, the proteins were transferred onto nitrocellulose membranes; the protein blots were stained with Ponceau S and then processed for immunostaining with anti-MBP <t>IgY.</t> The IgY antibody reacted with the MBP, but not with ferritin (Fn), BSA, or molecular mass markers. Also the MBP did not react with preimmune IgY (not shown). (B) Trophozoites (10 6 cells) were immunostained in cell suspension using anti-MBP IgY (left) or preimmune IgY (right) and FITC-labeled goat anti-chicken IgY. After being immunostained, the cells were smeared onto glass slides, fixed with 2.5% paraformaldehyde, and visualized under the confocal microscope.
    Horseradish Peroxidase Linked Goat Anti Chicken Igy, supplied by AvesLabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase linked goat anti chicken igy/product/AvesLabs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase linked goat anti chicken igy - by Bioz Stars, 2021-01
    85/100 stars
      Buy from Supplier

    92
    AvesLabs horseradish peroxidase
    Western blot analysis and immunohistochemical localization of Acanthamoeba <t>MBP.</t> (A) Affinity-purified amoeba MBP was electrophoresed under native conditions. After electrophoresis, the proteins were transferred onto nitrocellulose membranes; the protein blots were stained with Ponceau S and then processed for immunostaining with anti-MBP <t>IgY.</t> The IgY antibody reacted with the MBP, but not with ferritin (Fn), BSA, or molecular mass markers. Also the MBP did not react with preimmune IgY (not shown). (B) Trophozoites (10 6 cells) were immunostained in cell suspension using anti-MBP IgY (left) or preimmune IgY (right) and FITC-labeled goat anti-chicken IgY. After being immunostained, the cells were smeared onto glass slides, fixed with 2.5% paraformaldehyde, and visualized under the confocal microscope.
    Horseradish Peroxidase, supplied by AvesLabs, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase/product/AvesLabs
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    Image Search Results


    Specific binding of the chicken anti-MIP-133 antiserum. (A) Western blotting. Crude supernatants taken from A. castellanii trophozoites grown in mannose were resolved by SDS-PAGE and transferred to PVDF membranes. Membranes were first incubated with chicken anti-MIP-133 antiserum, followed by rabbit anti-chicken IgG-alkaline phosphatase, as described in Materials and Methods. The membranes were then washed and developed with Nitro Blue Tetrazolium-BCIP stock solution. The arrow indicates the 133-kDa band. (B) ELISA. Ninety-six-well plates were coated with 50 μg of MIP-133 and allowed to dry. After drying, wells were blocked and incubated with a 1:50, 1:75, or 1:100 dilution of either the chicken anti-MIP-133 antiserum or preimmune chicken antiserum. Wells were then washed and incubated with goat anti-chicken IgY-HRP. ELISA plates were developed and read at an OD at 405 nm. Bars and error bars represent the means ± SE of results from triplicate experiments. ***, significantly different from values obtained with preimmune serum at the same dilutions ( P

    Journal: Infection and Immunity

    Article Title: Pathogenic Acanthamoeba spp. Secrete a Mannose-Induced Cytolytic Protein That Correlates with the Ability To Cause Disease

    doi: 10.1128/IAI.71.11.6243-6255.2003

    Figure Lengend Snippet: Specific binding of the chicken anti-MIP-133 antiserum. (A) Western blotting. Crude supernatants taken from A. castellanii trophozoites grown in mannose were resolved by SDS-PAGE and transferred to PVDF membranes. Membranes were first incubated with chicken anti-MIP-133 antiserum, followed by rabbit anti-chicken IgG-alkaline phosphatase, as described in Materials and Methods. The membranes were then washed and developed with Nitro Blue Tetrazolium-BCIP stock solution. The arrow indicates the 133-kDa band. (B) ELISA. Ninety-six-well plates were coated with 50 μg of MIP-133 and allowed to dry. After drying, wells were blocked and incubated with a 1:50, 1:75, or 1:100 dilution of either the chicken anti-MIP-133 antiserum or preimmune chicken antiserum. Wells were then washed and incubated with goat anti-chicken IgY-HRP. ELISA plates were developed and read at an OD at 405 nm. Bars and error bars represent the means ± SE of results from triplicate experiments. ***, significantly different from values obtained with preimmune serum at the same dilutions ( P

    Article Snippet: HRP-conjugated goat anti-chicken IgY (Aveslabs) was added at a 1:10,000 dilution and incubated for 1 h. Plates were developed by adding 1.0 mM 2,2′-azinobis(3-ethyl-benzthiazoline-6-sulfonic acid) (Sigma) containing 0.003% H2 O2 and incubated for 30 min at room temperature.

    Techniques: Binding Assay, Western Blot, SDS Page, Incubation, Enzyme-linked Immunosorbent Assay

    Western blot analysis and immunohistochemical localization of Acanthamoeba MBP. (A) Affinity-purified amoeba MBP was electrophoresed under native conditions. After electrophoresis, the proteins were transferred onto nitrocellulose membranes; the protein blots were stained with Ponceau S and then processed for immunostaining with anti-MBP IgY. The IgY antibody reacted with the MBP, but not with ferritin (Fn), BSA, or molecular mass markers. Also the MBP did not react with preimmune IgY (not shown). (B) Trophozoites (10 6 cells) were immunostained in cell suspension using anti-MBP IgY (left) or preimmune IgY (right) and FITC-labeled goat anti-chicken IgY. After being immunostained, the cells were smeared onto glass slides, fixed with 2.5% paraformaldehyde, and visualized under the confocal microscope.

    Journal: Infection and Immunity

    Article Title: Biochemical Characterization and Functional Studies of Acanthamoeba Mannose-Binding Protein

    doi: 10.1128/IAI.73.9.5775-5781.2005

    Figure Lengend Snippet: Western blot analysis and immunohistochemical localization of Acanthamoeba MBP. (A) Affinity-purified amoeba MBP was electrophoresed under native conditions. After electrophoresis, the proteins were transferred onto nitrocellulose membranes; the protein blots were stained with Ponceau S and then processed for immunostaining with anti-MBP IgY. The IgY antibody reacted with the MBP, but not with ferritin (Fn), BSA, or molecular mass markers. Also the MBP did not react with preimmune IgY (not shown). (B) Trophozoites (10 6 cells) were immunostained in cell suspension using anti-MBP IgY (left) or preimmune IgY (right) and FITC-labeled goat anti-chicken IgY. After being immunostained, the cells were smeared onto glass slides, fixed with 2.5% paraformaldehyde, and visualized under the confocal microscope.

    Article Snippet: The protein blots were treated overnight at room temperature with 5% dry milk in PBS to block nonspecific binding sites and were then sequentially incubated with anti-MBP IgY (20 μg/ml; 37°C; 1 h), horseradish peroxidase-linked goat anti-chicken IgY (Aves Labs Inc.; 0.2 μg/ml; 37°C; 1 h), and a freshly prepared solution of diaminobenzidine-H2 O2 reagent (0.05% diaminobenzidine-0.01% H2 O2 -0.04% nickel chloride in Tris-HCl buffer, pH 7.2).

    Techniques: Western Blot, Immunohistochemistry, Affinity Purification, Electrophoresis, Staining, Immunostaining, Labeling, Microscopy

    Polyclonal anti-MBP IgY inhibits (i) adhesion of Acanthamoeba to Man-BSA and to epithelial cells and (ii) amoeba-induced cytopathic effect. Wells of microtiter plates coated with Man-BSA (A) or monolayer cultures of epithelial cells (B) were incubated with 35 S-labeled amoebae that had been pretreated with medium alone (Media), anti-MBP IgY (Anti-MBP), preimmune IgY (PI), or 100 mM α-Man. After being washed with PBS, the numbers of bound parasites were estimated as the radioactivity recovered from the wells. The means plus standard deviations of three experiments are shown ( n = 12 for each group). (C) Anti- Acanthamoeba MBP inhibits amoeba-induced CPE. Epithelial cells were cultured to confluence on 96-well plates and then incubated for 18 h with acanthamoebae that had been preincubated with either anti-MBP IgY, preimmune IgY, or 100 mM α-Man. After being washed, the cells were fixed, stained with Giemsa, and photographed (left). The approximate cell density in each well was estimated by using the Plot Density tool of Quantity One software (Bio-Rad, Hercules, CA). Clear, unstained regions indicate loss of cells (i and iii). Dark, stained areas indicate the presence of cells (ii and iv). Note that the amoeba-induced CPE of host cells was completely inhibited when the assays were performed using anti-MBP IgY-treated parasites (ii). Similar results were obtained regardless of whether the experiments were performed with rabbit corneal epithelial cells or Caco-2 cells.

    Journal: Infection and Immunity

    Article Title: Biochemical Characterization and Functional Studies of Acanthamoeba Mannose-Binding Protein

    doi: 10.1128/IAI.73.9.5775-5781.2005

    Figure Lengend Snippet: Polyclonal anti-MBP IgY inhibits (i) adhesion of Acanthamoeba to Man-BSA and to epithelial cells and (ii) amoeba-induced cytopathic effect. Wells of microtiter plates coated with Man-BSA (A) or monolayer cultures of epithelial cells (B) were incubated with 35 S-labeled amoebae that had been pretreated with medium alone (Media), anti-MBP IgY (Anti-MBP), preimmune IgY (PI), or 100 mM α-Man. After being washed with PBS, the numbers of bound parasites were estimated as the radioactivity recovered from the wells. The means plus standard deviations of three experiments are shown ( n = 12 for each group). (C) Anti- Acanthamoeba MBP inhibits amoeba-induced CPE. Epithelial cells were cultured to confluence on 96-well plates and then incubated for 18 h with acanthamoebae that had been preincubated with either anti-MBP IgY, preimmune IgY, or 100 mM α-Man. After being washed, the cells were fixed, stained with Giemsa, and photographed (left). The approximate cell density in each well was estimated by using the Plot Density tool of Quantity One software (Bio-Rad, Hercules, CA). Clear, unstained regions indicate loss of cells (i and iii). Dark, stained areas indicate the presence of cells (ii and iv). Note that the amoeba-induced CPE of host cells was completely inhibited when the assays were performed using anti-MBP IgY-treated parasites (ii). Similar results were obtained regardless of whether the experiments were performed with rabbit corneal epithelial cells or Caco-2 cells.

    Article Snippet: The protein blots were treated overnight at room temperature with 5% dry milk in PBS to block nonspecific binding sites and were then sequentially incubated with anti-MBP IgY (20 μg/ml; 37°C; 1 h), horseradish peroxidase-linked goat anti-chicken IgY (Aves Labs Inc.; 0.2 μg/ml; 37°C; 1 h), and a freshly prepared solution of diaminobenzidine-H2 O2 reagent (0.05% diaminobenzidine-0.01% H2 O2 -0.04% nickel chloride in Tris-HCl buffer, pH 7.2).

    Techniques: Incubation, Labeling, Radioactivity, Cell Culture, Staining, Software