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Santa Cruz Biotechnology horseradish peroxidase linked anti goat igg
Horseradish Peroxidase Linked Anti Goat Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/horseradish peroxidase linked anti goat igg/product/Santa Cruz Biotechnology
Average 90 stars, based on 2 article reviews
Price from $9.99 to $1999.99
horseradish peroxidase linked anti goat igg - by Bioz Stars, 2020-10
90/100 stars

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Western Blot:

Article Title: The lncRNA Neat1 promotes activation of inflammasomes in macrophages
Article Snippet: .. Antibodies and reagents Antibodies used for western bloting included anti-IL-1β (R & D Systems, 1:1000, AF-401-NA), anti-HIF-2α (R & D Systems, 1:1000, AF2997), anti-NLRP3 (Adipogen, 1:1000, AG-20B-0014), anti-ASC (Adipogen, 1:1000, AG-25B-0006), anti-caspase-1 (p20) (Adipogen, 1:1000, AG-20B-0042), anti-caspase-1 (p10) (Adipogen, 1:1000, AG-20B-0044), anti-p65 (Cell Signaling Technology, 1:1000, 8242), anti-HIF-1α (Cell Signaling Technology, 1:1000, 14179), anti-AIM2 (Cell Signaling Technology, 1:1000, 13095), anti-IL-6 (Cell Signaling Technology, 1:1000, 12912), anti-Actin (CMC-TAG, 1:2000, AT0009), anti-FLAG (Sigma-Aldrich, 1:2000, F3165), anti-Pspc1 (Santa Cruz, 1:200, sc-374181), anti-Sfpq (Santa Cruz, 1:200, sc-271796), anti-Nono (ABclonal, 1:500, A5282), anti-NLRC4 (ABclonal, 1:500, A7382), anti-histone H2A (abcam, 1:1000, ab177308), HRP-linked anti-rabbit IgG (Cell Signaling Technology, 1:2000, 7074), HRP-linked anti-mouse IgG (Cell Signaling Technology, 1:2000, 7076), HRP-linked anti-goat IgG (Santa Cruz, 1:1000, sc-2354). .. Antibodies used for immunoprecipitation included anti-NLRP3 (Cell Signaling Technology, 1:200, 15101), anti-caspase-1 (p20) (Adipogen, 1:200, AG-20B-0042), anti-ASC (Adipogen, 1:200, AG-25B-0006), anti-Nono (ABclonal, 1:50, A5282), normal rabbit IgG (Cell Signaling Technology, 1:200, 2729), normal mouse IgG (Santa Cruz, 1:80, sc-2025).

other:

Article Title: The RepID–CRL4 ubiquitin ligase complex regulates metaphase to anaphase transition via BUB3 degradation
Article Snippet: For secondary antibodies, horseradish peroxidase (HRP)-linked anti-mouse IgG (Cell Signaling, 7076), HRP-linked anti-rabbit IgG (Cell Signaling, 7074) and HRP-linked anti-goat IgG (Santa Cruz, sc-2020) were used following the manufacturer’s suggested protocols.

Article Title: ApoE4 Alters ABCA1 Membrane Trafficking in Astrocytes
Article Snippet: The following antibodies were used: anti-human ApoE antibody (Academy Bio-Medical, catalog #A14 50A-G1b), anti-ABCA1 antibody (Abcam, catalog #ab18180), anti-β-actin antibody (CST, catalog #3700), anti-ARF6 antibody (CST, catalog #5740), anti-β-Amyloid (6E10, BioLegend, catalog #803001), HRP-linked anti-mouse IgG (CST, catalog #7076), HRP-linked anti-rabbit IgG (CST, catalog #7074), HRP-linked anti-goat IgG (Santa Cruz Biotechnology, catalog #sc-2020).

Article Title: The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin
Article Snippet: For secondary antibodies, HRP-linked anti-mouse IgG (Cell Signaling, 7076), HRP-linked anti-rabbit IgG (Cell Signaling, 7074) and HRP-linked anti-goat IgG (Santa Cruz, sc-2020) were used following the manufacturer’s suggested protocols.

Article Title: Endothelial cell-secreted MIF reduces pericyte contractility and enhances neutrophil extravasation
Article Snippet: Horseradish peroxidase-linked anti-goat IgG (sc2020; Santa Cruz Biotechnology) and horseradish peroxidase-linked anti-rabbit IgG (7074; Cell Signaling Technology) were used as secondary antibodies.

Article Title: Insulin activates the rat sterol-regulatory-element-binding protein 1c (SREBP-1c) promoter through the combinatorial actions of SREBP, LXR, Sp-1 and NF-Y cis-acting elements
Article Snippet: Blots were probed for 1 h with 1 μg/ml goat polyclonal antiLXRα (Santa Cruz Biotechnology, Santa Cruz, CA) followed by 1 h with horseradish-peroxidase-linked anti-goat IgG (Santa Cruz Biotechnology).

Article Title: Multiple Interactions between Pullulanase Secreton Components Involved in Stabilization and Cytoplasmic Membrane Association of PulE
Article Snippet: The LexA-PulE′ hybrid was detected with anti-LexA primary antibodies (Santa Cruz Biotechnology) diluted 1:2,000 and horseradish peroxidase-linked anti-goat IgG diluted 1:10,000 (Santa Cruz Biotechnology).

Binding Assay:

Article Title: Glycogen Synthase Kinase 3 (GSK-3)-mediated Phosphorylation of Uracil N-Glycosylase 2 (UNG2) Facilitates the Repair of Floxuridine-induced DNA Lesions and Promotes Cell Survival *
Article Snippet: .. Antibodies were obtained from the following sources: mouse anti-β-actin (Sigma, A 5441, clone AC-15), rabbit anti-GSK-3α (Epitomics, 1742-1), goat anti-GSK-3β (L-17, Santa Cruz Biotechnology, sc-8257), mouse anti-HA.11 epitope tag (Covance, MMS-101R, lot 1), rabbit anti-histone H3 ( , Abcam, ab176842, lot GR206289-3), rabbit anti-phospho-PLK1 binding motif (ST*P, D73F6, Cell Signaling Technology, Danvers, MA, 5243S), mouse anti-Ser(P)139 -histone H2Ax (Millipore, 05-636, clone JBW301, lot JBC1881392), mouse anti-proliferating cell nuclear antigen (PC10, Santa Cruz Biotechnology, sc-56), mouse anti-S tag , rabbit anti-α-tubulin (Cell Signaling Technology, 2144), rabbit anti-UNG (Epitomics, 3130-1, lot YH030808C), HRP-linked anti-mouse immunoglobulin G and HRP-linked anti-rabbit immunoglobulin G (Cell Signaling Technology), HRP-linked anti-goat immunoglobulin G (Santa Cruz Biotechnology, sc-2354, lot K2009), and fluorescein isothiocyanate-conjugated goat anti-mouse immunoglobulin G (Jackson ImmunoResearch Laboratories, West Grove, PA). .. OVCAR-8 cells were a gift from D. Scudierio (NCI, National Institutes of Health), and HeLa and K562 cells were obtained from the American Type Culture Collection (Manassas, VA).

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  • 86
    Santa Cruz Biotechnology anti goat horseradish peroxidase linked igg
    Constitutive binding of <t>HuR</t> to the 5′UTR of Caspase-2 mRNA in DLD-1 cells. ( a ) An RNP-IP assay was performed in combination with RT-PCR from total cell lysates of DLD-1 cells to amplify mRNA species specifically bound by HuR. HuR-bound mRNA was precipitated by addition of a monoclonal HuR antibody (HuR) or, alternatively, the same amount of mouse <t>IgG</t> (IgG) as negative control. After elution from Sepharose beads, the isolated RNA samples were analyzed by RT-PCR using primer pairs, complementary and specific to the coding region of the indicated genes. The length of corresponding PCR products was determined by a DNA ladder. The specific immunoprecipitation (IP) of HuR is confirmed by western blot analysis (W.blot) shown in the right part of the panel. Data shown are representative of two independent experiments giving similar results. ( b , upper panel) Schematic representation of the different splice variants from human caspase-2 mRNA including caspase-2L (v1), caspase-2S (v2), and variant 3 (v3) encoding transcripts. The relative position of start (gray) and stop (dark) codons is depicted by triangles. Boxes show the relative position of exons and lacking exons are symbolized by a continuous line. The doubleheaded arrows indicate different regions (I, III and IV) encompassing variant-specific sequences (lines) or a region (II) commonly found in all three splice variants (dotted). ( b , lower panel) Crosslinking-RNP-IP analysis demonstrating that HuR specifically interacts with the 5′UTR of caspase-2L (v1) and splice variant 3, respectively. RNA-protein interactions were crosslinked with formaldehyde before HuR-bound mRNA was immunoprecipitated by addition of a HuR-specific antibody (HuR) or, alternatively, by the same amount of mouse IgG (IgG). The precipitated RNA samples were analyzed by RT-PCR by using primer pairs, spanning the indicated regions of caspase-2 mRNA (roman numerals). Input levels of different caspase-2 transcripts were isolated before the IP and were assessed by RT-PCR using the same primer pairs. ( c ) Biotin pull-down assay demonstrating a specific HuR binding to the 5′UTR of caspase-2. For pull-down assay, the biotinylated transcript encompassing 241 nucleotides of the 5′UTR of human caspase-2 (5′UTR Casp-2) was incubated with 300 μ g of total cell lysates from DLD-1 cells. The specific binding of HuR in the pull-down material was confirmed by western blotting. Biotinylated RNA from partial human reverse GAPDH (hrg) was used as a negative control, whereas 0.1 μ g of recombinant HuR (GST-HuR) instead of cell lysates was incubated with the same amount of biotinylated 5′UTR of caspase-2 and used as a positive control. Data shown are representative of two independent experiments giving similar results
    Anti Goat Horseradish Peroxidase Linked Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti goat horseradish peroxidase linked igg/product/Santa Cruz Biotechnology
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti goat horseradish peroxidase linked igg - by Bioz Stars, 2020-10
    86/100 stars
      Buy from Supplier

    88
    Santa Cruz Biotechnology horseradish peroxidase hrp linked goat anti rabbit igg
    Constitutive binding of <t>HuR</t> to the 5′UTR of Caspase-2 mRNA in DLD-1 cells. ( a ) An RNP-IP assay was performed in combination with RT-PCR from total cell lysates of DLD-1 cells to amplify mRNA species specifically bound by HuR. HuR-bound mRNA was precipitated by addition of a monoclonal HuR antibody (HuR) or, alternatively, the same amount of mouse <t>IgG</t> (IgG) as negative control. After elution from Sepharose beads, the isolated RNA samples were analyzed by RT-PCR using primer pairs, complementary and specific to the coding region of the indicated genes. The length of corresponding PCR products was determined by a DNA ladder. The specific immunoprecipitation (IP) of HuR is confirmed by western blot analysis (W.blot) shown in the right part of the panel. Data shown are representative of two independent experiments giving similar results. ( b , upper panel) Schematic representation of the different splice variants from human caspase-2 mRNA including caspase-2L (v1), caspase-2S (v2), and variant 3 (v3) encoding transcripts. The relative position of start (gray) and stop (dark) codons is depicted by triangles. Boxes show the relative position of exons and lacking exons are symbolized by a continuous line. The doubleheaded arrows indicate different regions (I, III and IV) encompassing variant-specific sequences (lines) or a region (II) commonly found in all three splice variants (dotted). ( b , lower panel) Crosslinking-RNP-IP analysis demonstrating that HuR specifically interacts with the 5′UTR of caspase-2L (v1) and splice variant 3, respectively. RNA-protein interactions were crosslinked with formaldehyde before HuR-bound mRNA was immunoprecipitated by addition of a HuR-specific antibody (HuR) or, alternatively, by the same amount of mouse IgG (IgG). The precipitated RNA samples were analyzed by RT-PCR by using primer pairs, spanning the indicated regions of caspase-2 mRNA (roman numerals). Input levels of different caspase-2 transcripts were isolated before the IP and were assessed by RT-PCR using the same primer pairs. ( c ) Biotin pull-down assay demonstrating a specific HuR binding to the 5′UTR of caspase-2. For pull-down assay, the biotinylated transcript encompassing 241 nucleotides of the 5′UTR of human caspase-2 (5′UTR Casp-2) was incubated with 300 μ g of total cell lysates from DLD-1 cells. The specific binding of HuR in the pull-down material was confirmed by western blotting. Biotinylated RNA from partial human reverse GAPDH (hrg) was used as a negative control, whereas 0.1 μ g of recombinant HuR (GST-HuR) instead of cell lysates was incubated with the same amount of biotinylated 5′UTR of caspase-2 and used as a positive control. Data shown are representative of two independent experiments giving similar results
    Horseradish Peroxidase Hrp Linked Goat Anti Rabbit Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp linked goat anti rabbit igg/product/Santa Cruz Biotechnology
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp linked goat anti rabbit igg - by Bioz Stars, 2020-10
    88/100 stars
      Buy from Supplier

    85
    Santa Cruz Biotechnology hrp linked anti goat igg secondary antibody
    Constitutive binding of <t>HuR</t> to the 5′UTR of Caspase-2 mRNA in DLD-1 cells. ( a ) An RNP-IP assay was performed in combination with RT-PCR from total cell lysates of DLD-1 cells to amplify mRNA species specifically bound by HuR. HuR-bound mRNA was precipitated by addition of a monoclonal HuR antibody (HuR) or, alternatively, the same amount of mouse <t>IgG</t> (IgG) as negative control. After elution from Sepharose beads, the isolated RNA samples were analyzed by RT-PCR using primer pairs, complementary and specific to the coding region of the indicated genes. The length of corresponding PCR products was determined by a DNA ladder. The specific immunoprecipitation (IP) of HuR is confirmed by western blot analysis (W.blot) shown in the right part of the panel. Data shown are representative of two independent experiments giving similar results. ( b , upper panel) Schematic representation of the different splice variants from human caspase-2 mRNA including caspase-2L (v1), caspase-2S (v2), and variant 3 (v3) encoding transcripts. The relative position of start (gray) and stop (dark) codons is depicted by triangles. Boxes show the relative position of exons and lacking exons are symbolized by a continuous line. The doubleheaded arrows indicate different regions (I, III and IV) encompassing variant-specific sequences (lines) or a region (II) commonly found in all three splice variants (dotted). ( b , lower panel) Crosslinking-RNP-IP analysis demonstrating that HuR specifically interacts with the 5′UTR of caspase-2L (v1) and splice variant 3, respectively. RNA-protein interactions were crosslinked with formaldehyde before HuR-bound mRNA was immunoprecipitated by addition of a HuR-specific antibody (HuR) or, alternatively, by the same amount of mouse IgG (IgG). The precipitated RNA samples were analyzed by RT-PCR by using primer pairs, spanning the indicated regions of caspase-2 mRNA (roman numerals). Input levels of different caspase-2 transcripts were isolated before the IP and were assessed by RT-PCR using the same primer pairs. ( c ) Biotin pull-down assay demonstrating a specific HuR binding to the 5′UTR of caspase-2. For pull-down assay, the biotinylated transcript encompassing 241 nucleotides of the 5′UTR of human caspase-2 (5′UTR Casp-2) was incubated with 300 μ g of total cell lysates from DLD-1 cells. The specific binding of HuR in the pull-down material was confirmed by western blotting. Biotinylated RNA from partial human reverse GAPDH (hrg) was used as a negative control, whereas 0.1 μ g of recombinant HuR (GST-HuR) instead of cell lysates was incubated with the same amount of biotinylated 5′UTR of caspase-2 and used as a positive control. Data shown are representative of two independent experiments giving similar results
    Hrp Linked Anti Goat Igg Secondary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp linked anti goat igg secondary antibody/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp linked anti goat igg secondary antibody - by Bioz Stars, 2020-10
    85/100 stars
      Buy from Supplier

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    Constitutive binding of HuR to the 5′UTR of Caspase-2 mRNA in DLD-1 cells. ( a ) An RNP-IP assay was performed in combination with RT-PCR from total cell lysates of DLD-1 cells to amplify mRNA species specifically bound by HuR. HuR-bound mRNA was precipitated by addition of a monoclonal HuR antibody (HuR) or, alternatively, the same amount of mouse IgG (IgG) as negative control. After elution from Sepharose beads, the isolated RNA samples were analyzed by RT-PCR using primer pairs, complementary and specific to the coding region of the indicated genes. The length of corresponding PCR products was determined by a DNA ladder. The specific immunoprecipitation (IP) of HuR is confirmed by western blot analysis (W.blot) shown in the right part of the panel. Data shown are representative of two independent experiments giving similar results. ( b , upper panel) Schematic representation of the different splice variants from human caspase-2 mRNA including caspase-2L (v1), caspase-2S (v2), and variant 3 (v3) encoding transcripts. The relative position of start (gray) and stop (dark) codons is depicted by triangles. Boxes show the relative position of exons and lacking exons are symbolized by a continuous line. The doubleheaded arrows indicate different regions (I, III and IV) encompassing variant-specific sequences (lines) or a region (II) commonly found in all three splice variants (dotted). ( b , lower panel) Crosslinking-RNP-IP analysis demonstrating that HuR specifically interacts with the 5′UTR of caspase-2L (v1) and splice variant 3, respectively. RNA-protein interactions were crosslinked with formaldehyde before HuR-bound mRNA was immunoprecipitated by addition of a HuR-specific antibody (HuR) or, alternatively, by the same amount of mouse IgG (IgG). The precipitated RNA samples were analyzed by RT-PCR by using primer pairs, spanning the indicated regions of caspase-2 mRNA (roman numerals). Input levels of different caspase-2 transcripts were isolated before the IP and were assessed by RT-PCR using the same primer pairs. ( c ) Biotin pull-down assay demonstrating a specific HuR binding to the 5′UTR of caspase-2. For pull-down assay, the biotinylated transcript encompassing 241 nucleotides of the 5′UTR of human caspase-2 (5′UTR Casp-2) was incubated with 300 μ g of total cell lysates from DLD-1 cells. The specific binding of HuR in the pull-down material was confirmed by western blotting. Biotinylated RNA from partial human reverse GAPDH (hrg) was used as a negative control, whereas 0.1 μ g of recombinant HuR (GST-HuR) instead of cell lysates was incubated with the same amount of biotinylated 5′UTR of caspase-2 and used as a positive control. Data shown are representative of two independent experiments giving similar results

    Journal: Cell Death & Disease

    Article Title: Attenuation of the ELAV1-like protein HuR sensitizes adenocarcinoma cells to the intrinsic apoptotic pathway by increasing the translation of caspase-2L

    doi: 10.1038/cddis.2014.279

    Figure Lengend Snippet: Constitutive binding of HuR to the 5′UTR of Caspase-2 mRNA in DLD-1 cells. ( a ) An RNP-IP assay was performed in combination with RT-PCR from total cell lysates of DLD-1 cells to amplify mRNA species specifically bound by HuR. HuR-bound mRNA was precipitated by addition of a monoclonal HuR antibody (HuR) or, alternatively, the same amount of mouse IgG (IgG) as negative control. After elution from Sepharose beads, the isolated RNA samples were analyzed by RT-PCR using primer pairs, complementary and specific to the coding region of the indicated genes. The length of corresponding PCR products was determined by a DNA ladder. The specific immunoprecipitation (IP) of HuR is confirmed by western blot analysis (W.blot) shown in the right part of the panel. Data shown are representative of two independent experiments giving similar results. ( b , upper panel) Schematic representation of the different splice variants from human caspase-2 mRNA including caspase-2L (v1), caspase-2S (v2), and variant 3 (v3) encoding transcripts. The relative position of start (gray) and stop (dark) codons is depicted by triangles. Boxes show the relative position of exons and lacking exons are symbolized by a continuous line. The doubleheaded arrows indicate different regions (I, III and IV) encompassing variant-specific sequences (lines) or a region (II) commonly found in all three splice variants (dotted). ( b , lower panel) Crosslinking-RNP-IP analysis demonstrating that HuR specifically interacts with the 5′UTR of caspase-2L (v1) and splice variant 3, respectively. RNA-protein interactions were crosslinked with formaldehyde before HuR-bound mRNA was immunoprecipitated by addition of a HuR-specific antibody (HuR) or, alternatively, by the same amount of mouse IgG (IgG). The precipitated RNA samples were analyzed by RT-PCR by using primer pairs, spanning the indicated regions of caspase-2 mRNA (roman numerals). Input levels of different caspase-2 transcripts were isolated before the IP and were assessed by RT-PCR using the same primer pairs. ( c ) Biotin pull-down assay demonstrating a specific HuR binding to the 5′UTR of caspase-2. For pull-down assay, the biotinylated transcript encompassing 241 nucleotides of the 5′UTR of human caspase-2 (5′UTR Casp-2) was incubated with 300 μ g of total cell lysates from DLD-1 cells. The specific binding of HuR in the pull-down material was confirmed by western blotting. Biotinylated RNA from partial human reverse GAPDH (hrg) was used as a negative control, whereas 0.1 μ g of recombinant HuR (GST-HuR) instead of cell lysates was incubated with the same amount of biotinylated 5′UTR of caspase-2 and used as a positive control. Data shown are representative of two independent experiments giving similar results

    Article Snippet: Antibodies raised against HuR, anti-rabbit, anti-mouse and anti-goat horseradish peroxidase linked IgG were purchased from Santa Cruz Biotechnology (Heidelberg, Germany).

    Techniques: Binding Assay, Reverse Transcription Polymerase Chain Reaction, Negative Control, Isolation, Polymerase Chain Reaction, Immunoprecipitation, Western Blot, Variant Assay, Pull Down Assay, Incubation, Recombinant, Positive Control