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Santa Cruz Biotechnology horseradish peroxidase linked anti goat igg
Horseradish Peroxidase Linked Anti Goat Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/horseradish peroxidase linked anti goat igg/product/Santa Cruz Biotechnology
Average 82 stars, based on 2 article reviews
Price from $9.99 to $1999.99
horseradish peroxidase linked anti goat igg - by Bioz Stars, 2020-02
82/100 stars

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Nucleic Acid Electrophoresis:

Article Title: Multiple Interactions between Pullulanase Secreton Components Involved in Stabilization and Cytoplasmic Membrane Association of PulE
Article Snippet: Paragraph title: Gel electrophoresis and immunoblotting. ... The LexA-PulE′ hybrid was detected with anti-LexA primary antibodies (Santa Cruz Biotechnology) diluted 1:2,000 and horseradish peroxidase-linked anti-goat IgG diluted 1:10,000 (Santa Cruz Biotechnology).

Flow Cytometry:

Article Title: Spatial Distribution and Receptor Specificity of Zebrafish Kit System - Evidence for a Kit-Mediated Bi-Directional Communication System in the Preovulatory Ovarian Follicle
Article Snippet: Animals and chemicals Zebrafish (Danio rerio ) were obtained from a local tropical fish market and maintained in flow-through aquaria at 28±1°C on a photoperiod of 14L∶10D, with light on at 8:00. .. Antibodies for phospho-KIT (#sc-18076), HRP-linked anti-goat IgG (#sc-2056) and HRP-linked anti-rabbit IgG (#sc-2374) were from Santa Cruz Biotechnology (Santa Cruz, CA) and those for β-actin (#4967L), p44/42 MAP Kinase (#9102L) and phospho-p44/42 MAP Kinase (#9101L) were from Cell Signaling Technology (Danvers, MA).

Article Title: The lncRNA Neat1 promotes activation of inflammasomes in macrophages
Article Snippet: Antibodies and reagents Antibodies used for western bloting included anti-IL-1β (R & D Systems, 1:1000, AF-401-NA), anti-HIF-2α (R & D Systems, 1:1000, AF2997), anti-NLRP3 (Adipogen, 1:1000, AG-20B-0014), anti-ASC (Adipogen, 1:1000, AG-25B-0006), anti-caspase-1 (p20) (Adipogen, 1:1000, AG-20B-0042), anti-caspase-1 (p10) (Adipogen, 1:1000, AG-20B-0044), anti-p65 (Cell Signaling Technology, 1:1000, 8242), anti-HIF-1α (Cell Signaling Technology, 1:1000, 14179), anti-AIM2 (Cell Signaling Technology, 1:1000, 13095), anti-IL-6 (Cell Signaling Technology, 1:1000, 12912), anti-Actin (CMC-TAG, 1:2000, AT0009), anti-FLAG (Sigma-Aldrich, 1:2000, F3165), anti-Pspc1 (Santa Cruz, 1:200, sc-374181), anti-Sfpq (Santa Cruz, 1:200, sc-271796), anti-Nono (ABclonal, 1:500, A5282), anti-NLRC4 (ABclonal, 1:500, A7382), anti-histone H2A (abcam, 1:1000, ab177308), HRP-linked anti-rabbit IgG (Cell Signaling Technology, 1:2000, 7074), HRP-linked anti-mouse IgG (Cell Signaling Technology, 1:2000, 7076), HRP-linked anti-goat IgG (Santa Cruz, 1:1000, sc-2354). .. PerCP-CyTM 5.5 anti-mouse CD11b (BD Biosciences, 1:1000, 550993), APC anti-mouse Ly-6G (BD Biosciences, 1:1000, 560599), and PE anti-mouse Ly-6C (BD Biosciences, 1:1000, 560592) were used for flow cytometry.

Protease Inhibitor:

Article Title: A novel tag-free probe for targeting molecules interacting with a flavonoid catabolite
Article Snippet: 2.1 Materials DOPAC, laccase from Rhus vernicifera , glyceraldehyde-3-phosphate dehydrogenase (GAPDH), tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA), bis(4-nitrophenyl) phosphate (BNPP) and azide-PEG3 -biotin conjugate were obtained from Sigma Aldrich (St. Louis, USA). p -Toluensulfonic acid monohydrate (PTSA), n -butanol, toluene, copper (II) sulfate pentahydrate, protease inhibitor cocktail and Chemi-Lumi One Super were purchased from nacalai tasque (Kyoto, Japan). .. Anti-actin antibody, anti-aryl hydrocarbon receptor (AhR) antibody, anti-Keap1 antibody, horseradish peroxidase-linked anti-mouse IgG and horseradish peroxidase-linked anti-goat IgG were purchased from Santa Cruz Biotechnology (Santa Cruz, USA).

Article Title: Insulin activates the rat sterol-regulatory-element-binding protein 1c (SREBP-1c) promoter through the combinatorial actions of SREBP, LXR, Sp-1 and NF-Y cis-acting elements
Article Snippet: The pelleted nuclei were resuspended in 1 ml of nuclear extraction buffer [20 mM Hepes (pH 7.9), 20% glycerol, 1% NP40, 0.1% Triton X-100, 420 mM NaCl, 100 mM KCl, 10 mM MgCl2 , 1 mM EGTA, 1 mM EDTA, 1 mM DTT, 1 mM PMSF and protease inhibitor cocktail (Sigma)]. .. Blots were probed for 1 h with 1 μg/ml goat polyclonal antiLXRα (Santa Cruz Biotechnology, Santa Cruz, CA) followed by 1 h with horseradish-peroxidase-linked anti-goat IgG (Santa Cruz Biotechnology).

Western Blot:

Article Title: The lncRNA Neat1 promotes activation of inflammasomes in macrophages
Article Snippet: .. Antibodies and reagents Antibodies used for western bloting included anti-IL-1β (R & D Systems, 1:1000, AF-401-NA), anti-HIF-2α (R & D Systems, 1:1000, AF2997), anti-NLRP3 (Adipogen, 1:1000, AG-20B-0014), anti-ASC (Adipogen, 1:1000, AG-25B-0006), anti-caspase-1 (p20) (Adipogen, 1:1000, AG-20B-0042), anti-caspase-1 (p10) (Adipogen, 1:1000, AG-20B-0044), anti-p65 (Cell Signaling Technology, 1:1000, 8242), anti-HIF-1α (Cell Signaling Technology, 1:1000, 14179), anti-AIM2 (Cell Signaling Technology, 1:1000, 13095), anti-IL-6 (Cell Signaling Technology, 1:1000, 12912), anti-Actin (CMC-TAG, 1:2000, AT0009), anti-FLAG (Sigma-Aldrich, 1:2000, F3165), anti-Pspc1 (Santa Cruz, 1:200, sc-374181), anti-Sfpq (Santa Cruz, 1:200, sc-271796), anti-Nono (ABclonal, 1:500, A5282), anti-NLRC4 (ABclonal, 1:500, A7382), anti-histone H2A (abcam, 1:1000, ab177308), HRP-linked anti-rabbit IgG (Cell Signaling Technology, 1:2000, 7074), HRP-linked anti-mouse IgG (Cell Signaling Technology, 1:2000, 7076), HRP-linked anti-goat IgG (Santa Cruz, 1:1000, sc-2354). .. Antibodies used for immunoprecipitation included anti-NLRP3 (Cell Signaling Technology, 1:200, 15101), anti-caspase-1 (p20) (Adipogen, 1:200, AG-20B-0042), anti-ASC (Adipogen, 1:200, AG-25B-0006), anti-Nono (ABclonal, 1:50, A5282), normal rabbit IgG (Cell Signaling Technology, 1:200, 2729), normal mouse IgG (Santa Cruz, 1:80, sc-2025).

Article Title: The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin
Article Snippet: Paragraph title: Chromatin fractionation, co-IP and Western blotting ... For secondary antibodies, HRP-linked anti-mouse IgG (Cell Signaling, 7076), HRP-linked anti-rabbit IgG (Cell Signaling, 7074) and HRP-linked anti-goat IgG (Santa Cruz, sc-2020) were used following the manufacturer’s suggested protocols.

Article Title: Insulin activates the rat sterol-regulatory-element-binding protein 1c (SREBP-1c) promoter through the combinatorial actions of SREBP, LXR, Sp-1 and NF-Y cis-acting elements
Article Snippet: Paragraph title: Measurement of LXR protein levels by Western blot analysis ... Blots were probed for 1 h with 1 μg/ml goat polyclonal antiLXRα (Santa Cruz Biotechnology, Santa Cruz, CA) followed by 1 h with horseradish-peroxidase-linked anti-goat IgG (Santa Cruz Biotechnology).

Article Title: Phosphorylated SIRT1 associates with replication origins to prevent excess replication initiation and preserve genomic stability
Article Snippet: Paragraph title: Chromatin binding assays and western blotting ... For secondary antibody, HRP linked anti-mouse IgG (Cell Signaling, 7076), HRP linked anti-rabbit IgG (Cell Signaling, 7074), HRP linked anti-goat IgG (SantaCruz, sc-2020) were used following the manufacturer's suggested protocol.

Cytometry:

Article Title: The lncRNA Neat1 promotes activation of inflammasomes in macrophages
Article Snippet: Antibodies and reagents Antibodies used for western bloting included anti-IL-1β (R & D Systems, 1:1000, AF-401-NA), anti-HIF-2α (R & D Systems, 1:1000, AF2997), anti-NLRP3 (Adipogen, 1:1000, AG-20B-0014), anti-ASC (Adipogen, 1:1000, AG-25B-0006), anti-caspase-1 (p20) (Adipogen, 1:1000, AG-20B-0042), anti-caspase-1 (p10) (Adipogen, 1:1000, AG-20B-0044), anti-p65 (Cell Signaling Technology, 1:1000, 8242), anti-HIF-1α (Cell Signaling Technology, 1:1000, 14179), anti-AIM2 (Cell Signaling Technology, 1:1000, 13095), anti-IL-6 (Cell Signaling Technology, 1:1000, 12912), anti-Actin (CMC-TAG, 1:2000, AT0009), anti-FLAG (Sigma-Aldrich, 1:2000, F3165), anti-Pspc1 (Santa Cruz, 1:200, sc-374181), anti-Sfpq (Santa Cruz, 1:200, sc-271796), anti-Nono (ABclonal, 1:500, A5282), anti-NLRC4 (ABclonal, 1:500, A7382), anti-histone H2A (abcam, 1:1000, ab177308), HRP-linked anti-rabbit IgG (Cell Signaling Technology, 1:2000, 7074), HRP-linked anti-mouse IgG (Cell Signaling Technology, 1:2000, 7076), HRP-linked anti-goat IgG (Santa Cruz, 1:1000, sc-2354). .. PerCP-CyTM 5.5 anti-mouse CD11b (BD Biosciences, 1:1000, 550993), APC anti-mouse Ly-6G (BD Biosciences, 1:1000, 560599), and PE anti-mouse Ly-6C (BD Biosciences, 1:1000, 560592) were used for flow cytometry.

Immunofluorescence:

Article Title: The lncRNA Neat1 promotes activation of inflammasomes in macrophages
Article Snippet: Antibodies and reagents Antibodies used for western bloting included anti-IL-1β (R & D Systems, 1:1000, AF-401-NA), anti-HIF-2α (R & D Systems, 1:1000, AF2997), anti-NLRP3 (Adipogen, 1:1000, AG-20B-0014), anti-ASC (Adipogen, 1:1000, AG-25B-0006), anti-caspase-1 (p20) (Adipogen, 1:1000, AG-20B-0042), anti-caspase-1 (p10) (Adipogen, 1:1000, AG-20B-0044), anti-p65 (Cell Signaling Technology, 1:1000, 8242), anti-HIF-1α (Cell Signaling Technology, 1:1000, 14179), anti-AIM2 (Cell Signaling Technology, 1:1000, 13095), anti-IL-6 (Cell Signaling Technology, 1:1000, 12912), anti-Actin (CMC-TAG, 1:2000, AT0009), anti-FLAG (Sigma-Aldrich, 1:2000, F3165), anti-Pspc1 (Santa Cruz, 1:200, sc-374181), anti-Sfpq (Santa Cruz, 1:200, sc-271796), anti-Nono (ABclonal, 1:500, A5282), anti-NLRC4 (ABclonal, 1:500, A7382), anti-histone H2A (abcam, 1:1000, ab177308), HRP-linked anti-rabbit IgG (Cell Signaling Technology, 1:2000, 7074), HRP-linked anti-mouse IgG (Cell Signaling Technology, 1:2000, 7076), HRP-linked anti-goat IgG (Santa Cruz, 1:1000, sc-2354). .. Anti-ASC (Santa Cruz, 1:50, sc-22514), anti-Pspc1 (mouse IgG, Santa Cruz, 1:50, sc-374181), anti-Sfpq (mouse IgG, Santa Cruz, 1:50, sc-271796), anti-Sfpq (rabbit IgG, Proteintech, 1:50, 15585-1-AP), anti-Nono (rabbit IgG, ABclonal, 1:50, A5282), Alexa fluor 488 donkey anti-rabbit IgG(H + L) (Life Technologies, 1:1000, A21206), Alexa fluor 568 donkey anti-mouse IgG(H + L) (Life Technologies, 1:1000, A10037), and Alexa fluor 647 donkey anti-rabbit IgG(H + L) (Life Technologies, 1:1000, A31573) were used for immunofluorescence.

Immunoprecipitation:

Article Title: The lncRNA Neat1 promotes activation of inflammasomes in macrophages
Article Snippet: Antibodies and reagents Antibodies used for western bloting included anti-IL-1β (R & D Systems, 1:1000, AF-401-NA), anti-HIF-2α (R & D Systems, 1:1000, AF2997), anti-NLRP3 (Adipogen, 1:1000, AG-20B-0014), anti-ASC (Adipogen, 1:1000, AG-25B-0006), anti-caspase-1 (p20) (Adipogen, 1:1000, AG-20B-0042), anti-caspase-1 (p10) (Adipogen, 1:1000, AG-20B-0044), anti-p65 (Cell Signaling Technology, 1:1000, 8242), anti-HIF-1α (Cell Signaling Technology, 1:1000, 14179), anti-AIM2 (Cell Signaling Technology, 1:1000, 13095), anti-IL-6 (Cell Signaling Technology, 1:1000, 12912), anti-Actin (CMC-TAG, 1:2000, AT0009), anti-FLAG (Sigma-Aldrich, 1:2000, F3165), anti-Pspc1 (Santa Cruz, 1:200, sc-374181), anti-Sfpq (Santa Cruz, 1:200, sc-271796), anti-Nono (ABclonal, 1:500, A5282), anti-NLRC4 (ABclonal, 1:500, A7382), anti-histone H2A (abcam, 1:1000, ab177308), HRP-linked anti-rabbit IgG (Cell Signaling Technology, 1:2000, 7074), HRP-linked anti-mouse IgG (Cell Signaling Technology, 1:2000, 7076), HRP-linked anti-goat IgG (Santa Cruz, 1:1000, sc-2354). .. Antibodies used for immunoprecipitation included anti-NLRP3 (Cell Signaling Technology, 1:200, 15101), anti-caspase-1 (p20) (Adipogen, 1:200, AG-20B-0042), anti-ASC (Adipogen, 1:200, AG-25B-0006), anti-Nono (ABclonal, 1:50, A5282), normal rabbit IgG (Cell Signaling Technology, 1:200, 2729), normal mouse IgG (Santa Cruz, 1:80, sc-2025).

Incubation:

Article Title: Multiple Interactions between Pullulanase Secreton Components Involved in Stabilization and Cytoplasmic Membrane Association of PulE
Article Snippet: Pul proteins and DjlA were detected by incubation with specific primary antisera diluted to 1:2,000 to 1:10,000, followed by secondary horseradish peroxidase-linked anti-rabbit immunoglobulin G (IgG) (Amersham-Pharmacia; 1:10,000) and enhanced chemiluminescence (ECL kit; Amersham-Pharmacia). .. The LexA-PulE′ hybrid was detected with anti-LexA primary antibodies (Santa Cruz Biotechnology) diluted 1:2,000 and horseradish peroxidase-linked anti-goat IgG diluted 1:10,000 (Santa Cruz Biotechnology).

Fractionation:

Article Title: The RepID–CRL4 ubiquitin ligase complex regulates metaphase to anaphase transition via BUB3 degradation
Article Snippet: Paragraph title: Chromatin fractionation, co-IP and immunoblotting ... For secondary antibodies, horseradish peroxidase (HRP)-linked anti-mouse IgG (Cell Signaling, 7076), HRP-linked anti-rabbit IgG (Cell Signaling, 7074) and HRP-linked anti-goat IgG (Santa Cruz, sc-2020) were used following the manufacturer’s suggested protocols.

Article Title: The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin
Article Snippet: Paragraph title: Chromatin fractionation, co-IP and Western blotting ... For secondary antibodies, HRP-linked anti-mouse IgG (Cell Signaling, 7076), HRP-linked anti-rabbit IgG (Cell Signaling, 7074) and HRP-linked anti-goat IgG (Santa Cruz, sc-2020) were used following the manufacturer’s suggested protocols.

Polyacrylamide Gel Electrophoresis:

Article Title: Multiple Interactions between Pullulanase Secreton Components Involved in Stabilization and Cytoplasmic Membrane Association of PulE
Article Snippet: After solubilization in sample buffer containing 2% sodium dodecyl sulfate (SDS) and heating at 100°C, proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) and transferred onto nitrocellulose. .. The LexA-PulE′ hybrid was detected with anti-LexA primary antibodies (Santa Cruz Biotechnology) diluted 1:2,000 and horseradish peroxidase-linked anti-goat IgG diluted 1:10,000 (Santa Cruz Biotechnology).

Co-Immunoprecipitation Assay:

Article Title: The RepID–CRL4 ubiquitin ligase complex regulates metaphase to anaphase transition via BUB3 degradation
Article Snippet: Paragraph title: Chromatin fractionation, co-IP and immunoblotting ... For secondary antibodies, horseradish peroxidase (HRP)-linked anti-mouse IgG (Cell Signaling, 7076), HRP-linked anti-rabbit IgG (Cell Signaling, 7074) and HRP-linked anti-goat IgG (Santa Cruz, sc-2020) were used following the manufacturer’s suggested protocols.

Article Title: The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin
Article Snippet: Paragraph title: Chromatin fractionation, co-IP and Western blotting ... For secondary antibodies, HRP-linked anti-mouse IgG (Cell Signaling, 7076), HRP-linked anti-rabbit IgG (Cell Signaling, 7074) and HRP-linked anti-goat IgG (Santa Cruz, sc-2020) were used following the manufacturer’s suggested protocols.

Lysis:

Article Title: Insulin activates the rat sterol-regulatory-element-binding protein 1c (SREBP-1c) promoter through the combinatorial actions of SREBP, LXR, Sp-1 and NF-Y cis-acting elements
Article Snippet: Centrifuged cells were resuspended in 1 ml of lysis buffer [20 mM Hepes (pH 7.9), 20% glycerol, 1% NP40 (Nonidet P40), 0.1% Triton X-100, 10 mM NaCl, 1.5 mM MgCl2 , 1 mM EGTA, 1 mM EDTA, 1 mM DTT, 1 mM PMSF and protease inhibitor cocktail (Sigma)]. .. Blots were probed for 1 h with 1 μg/ml goat polyclonal antiLXRα (Santa Cruz Biotechnology, Santa Cruz, CA) followed by 1 h with horseradish-peroxidase-linked anti-goat IgG (Santa Cruz Biotechnology).

Centrifugation:

Article Title: The RepID–CRL4 ubiquitin ligase complex regulates metaphase to anaphase transition via BUB3 degradation
Article Snippet: The protein–bead complexes were collected by centrifugation at 1700 × g for 3 min and washed three times with PBS. .. For secondary antibodies, horseradish peroxidase (HRP)-linked anti-mouse IgG (Cell Signaling, 7076), HRP-linked anti-rabbit IgG (Cell Signaling, 7074) and HRP-linked anti-goat IgG (Santa Cruz, sc-2020) were used following the manufacturer’s suggested protocols.

Article Title: The replication initiation determinant protein (RepID) modulates replication by recruiting CUL4 to chromatin
Article Snippet: The protein-bead complexes were collected by centrifugation at 1700×g for 3 min and washed three times with PBS. .. For secondary antibodies, HRP-linked anti-mouse IgG (Cell Signaling, 7076), HRP-linked anti-rabbit IgG (Cell Signaling, 7074) and HRP-linked anti-goat IgG (Santa Cruz, sc-2020) were used following the manufacturer’s suggested protocols.

Binding Assay:

Article Title: Glycogen Synthase Kinase 3 (GSK-3)-mediated Phosphorylation of Uracil N-Glycosylase 2 (UNG2) Facilitates the Repair of Floxuridine-induced DNA Lesions and Promotes Cell Survival *
Article Snippet: .. Antibodies were obtained from the following sources: mouse anti-β-actin (Sigma, A 5441, clone AC-15), rabbit anti-GSK-3α (Epitomics, 1742-1), goat anti-GSK-3β (L-17, Santa Cruz Biotechnology, sc-8257), mouse anti-HA.11 epitope tag (Covance, MMS-101R, lot 1), rabbit anti-histone H3 ( , Abcam, ab176842, lot GR206289-3), rabbit anti-phospho-PLK1 binding motif (ST*P, D73F6, Cell Signaling Technology, Danvers, MA, 5243S), mouse anti-Ser(P)139 -histone H2Ax (Millipore, 05-636, clone JBW301, lot JBC1881392), mouse anti-proliferating cell nuclear antigen (PC10, Santa Cruz Biotechnology, sc-56), mouse anti-S tag , rabbit anti-α-tubulin (Cell Signaling Technology, 2144), rabbit anti-UNG (Epitomics, 3130-1, lot YH030808C), HRP-linked anti-mouse immunoglobulin G and HRP-linked anti-rabbit immunoglobulin G (Cell Signaling Technology), HRP-linked anti-goat immunoglobulin G (Santa Cruz Biotechnology, sc-2354, lot K2009), and fluorescein isothiocyanate-conjugated goat anti-mouse immunoglobulin G (Jackson ImmunoResearch Laboratories, West Grove, PA). .. OVCAR-8 cells were a gift from D. Scudierio (NCI, National Institutes of Health), and HeLa and K562 cells were obtained from the American Type Culture Collection (Manassas, VA).

Article Title: Phosphorylated SIRT1 associates with replication origins to prevent excess replication initiation and preserve genomic stability
Article Snippet: Paragraph title: Chromatin binding assays and western blotting ... For secondary antibody, HRP linked anti-mouse IgG (Cell Signaling, 7076), HRP linked anti-rabbit IgG (Cell Signaling, 7074), HRP linked anti-goat IgG (SantaCruz, sc-2020) were used following the manufacturer's suggested protocol.

Fluorescence In Situ Hybridization:

Article Title: Spatial Distribution and Receptor Specificity of Zebrafish Kit System - Evidence for a Kit-Mediated Bi-Directional Communication System in the Preovulatory Ovarian Follicle
Article Snippet: The fish was fed twice a day with the commercial tropical fish feed Otohime S1 (Marubeni Nisshin Feed Co., Tokyo, Japan) and once with frozen artemia. .. Antibodies for phospho-KIT (#sc-18076), HRP-linked anti-goat IgG (#sc-2056) and HRP-linked anti-rabbit IgG (#sc-2374) were from Santa Cruz Biotechnology (Santa Cruz, CA) and those for β-actin (#4967L), p44/42 MAP Kinase (#9102L) and phospho-p44/42 MAP Kinase (#9101L) were from Cell Signaling Technology (Danvers, MA).

SDS Page:

Article Title: Insulin activates the rat sterol-regulatory-element-binding protein 1c (SREBP-1c) promoter through the combinatorial actions of SREBP, LXR, Sp-1 and NF-Y cis-acting elements
Article Snippet: Samples containing 50 μg of nuclear protein were dissolved in Laemmli buffer, and the proteins were resolved by SDS/PAGE (7.5% gel), and transferred on to nitrocellulose membranes. .. Blots were probed for 1 h with 1 μg/ml goat polyclonal antiLXRα (Santa Cruz Biotechnology, Santa Cruz, CA) followed by 1 h with horseradish-peroxidase-linked anti-goat IgG (Santa Cruz Biotechnology).

Isolation:

Article Title: Phosphorylated SIRT1 associates with replication origins to prevent excess replication initiation and preserve genomic stability
Article Snippet: For tight chromatin binding assays, nuclei were isolated and further fractionated by sequential extractions with increasing salt concentrations (0.3, 0.45, 0.6, 1.2 and 1.8 M), 10 min each at 4°C with gentle rotation. .. For secondary antibody, HRP linked anti-mouse IgG (Cell Signaling, 7076), HRP linked anti-rabbit IgG (Cell Signaling, 7074), HRP linked anti-goat IgG (SantaCruz, sc-2020) were used following the manufacturer's suggested protocol.

Staining:

Article Title: Multiple Interactions between Pullulanase Secreton Components Involved in Stabilization and Cytoplasmic Membrane Association of PulE
Article Snippet: Ponceau red staining allowed visualization of porins (outer membrane markers) in membrane fractions. .. The LexA-PulE′ hybrid was detected with anti-LexA primary antibodies (Santa Cruz Biotechnology) diluted 1:2,000 and horseradish peroxidase-linked anti-goat IgG diluted 1:10,000 (Santa Cruz Biotechnology).

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    Santa Cruz Biotechnology anti goat horseradish peroxidase linked igg
    Constitutive binding of <t>HuR</t> to the 5′UTR of Caspase-2 mRNA in DLD-1 cells. ( a ) An RNP-IP assay was performed in combination with RT-PCR from total cell lysates of DLD-1 cells to amplify mRNA species specifically bound by HuR. HuR-bound mRNA was precipitated by addition of a monoclonal HuR antibody (HuR) or, alternatively, the same amount of mouse <t>IgG</t> (IgG) as negative control. After elution from Sepharose beads, the isolated RNA samples were analyzed by RT-PCR using primer pairs, complementary and specific to the coding region of the indicated genes. The length of corresponding PCR products was determined by a DNA ladder. The specific immunoprecipitation (IP) of HuR is confirmed by western blot analysis (W.blot) shown in the right part of the panel. Data shown are representative of two independent experiments giving similar results. ( b , upper panel) Schematic representation of the different splice variants from human caspase-2 mRNA including caspase-2L (v1), caspase-2S (v2), and variant 3 (v3) encoding transcripts. The relative position of start (gray) and stop (dark) codons is depicted by triangles. Boxes show the relative position of exons and lacking exons are symbolized by a continuous line. The doubleheaded arrows indicate different regions (I, III and IV) encompassing variant-specific sequences (lines) or a region (II) commonly found in all three splice variants (dotted). ( b , lower panel) Crosslinking-RNP-IP analysis demonstrating that HuR specifically interacts with the 5′UTR of caspase-2L (v1) and splice variant 3, respectively. RNA-protein interactions were crosslinked with formaldehyde before HuR-bound mRNA was immunoprecipitated by addition of a HuR-specific antibody (HuR) or, alternatively, by the same amount of mouse IgG (IgG). The precipitated RNA samples were analyzed by RT-PCR by using primer pairs, spanning the indicated regions of caspase-2 mRNA (roman numerals). Input levels of different caspase-2 transcripts were isolated before the IP and were assessed by RT-PCR using the same primer pairs. ( c ) Biotin pull-down assay demonstrating a specific HuR binding to the 5′UTR of caspase-2. For pull-down assay, the biotinylated transcript encompassing 241 nucleotides of the 5′UTR of human caspase-2 (5′UTR Casp-2) was incubated with 300 μ g of total cell lysates from DLD-1 cells. The specific binding of HuR in the pull-down material was confirmed by western blotting. Biotinylated RNA from partial human reverse GAPDH (hrg) was used as a negative control, whereas 0.1 μ g of recombinant HuR (GST-HuR) instead of cell lysates was incubated with the same amount of biotinylated 5′UTR of caspase-2 and used as a positive control. Data shown are representative of two independent experiments giving similar results
    Anti Goat Horseradish Peroxidase Linked Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti goat horseradish peroxidase linked igg/product/Santa Cruz Biotechnology
    Average 75 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti goat horseradish peroxidase linked igg - by Bioz Stars, 2020-02
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    Santa Cruz Biotechnology horseradish peroxidase hrp linked goat anti rabbit igg
    Constitutive binding of <t>HuR</t> to the 5′UTR of Caspase-2 mRNA in DLD-1 cells. ( a ) An RNP-IP assay was performed in combination with RT-PCR from total cell lysates of DLD-1 cells to amplify mRNA species specifically bound by HuR. HuR-bound mRNA was precipitated by addition of a monoclonal HuR antibody (HuR) or, alternatively, the same amount of mouse <t>IgG</t> (IgG) as negative control. After elution from Sepharose beads, the isolated RNA samples were analyzed by RT-PCR using primer pairs, complementary and specific to the coding region of the indicated genes. The length of corresponding PCR products was determined by a DNA ladder. The specific immunoprecipitation (IP) of HuR is confirmed by western blot analysis (W.blot) shown in the right part of the panel. Data shown are representative of two independent experiments giving similar results. ( b , upper panel) Schematic representation of the different splice variants from human caspase-2 mRNA including caspase-2L (v1), caspase-2S (v2), and variant 3 (v3) encoding transcripts. The relative position of start (gray) and stop (dark) codons is depicted by triangles. Boxes show the relative position of exons and lacking exons are symbolized by a continuous line. The doubleheaded arrows indicate different regions (I, III and IV) encompassing variant-specific sequences (lines) or a region (II) commonly found in all three splice variants (dotted). ( b , lower panel) Crosslinking-RNP-IP analysis demonstrating that HuR specifically interacts with the 5′UTR of caspase-2L (v1) and splice variant 3, respectively. RNA-protein interactions were crosslinked with formaldehyde before HuR-bound mRNA was immunoprecipitated by addition of a HuR-specific antibody (HuR) or, alternatively, by the same amount of mouse IgG (IgG). The precipitated RNA samples were analyzed by RT-PCR by using primer pairs, spanning the indicated regions of caspase-2 mRNA (roman numerals). Input levels of different caspase-2 transcripts were isolated before the IP and were assessed by RT-PCR using the same primer pairs. ( c ) Biotin pull-down assay demonstrating a specific HuR binding to the 5′UTR of caspase-2. For pull-down assay, the biotinylated transcript encompassing 241 nucleotides of the 5′UTR of human caspase-2 (5′UTR Casp-2) was incubated with 300 μ g of total cell lysates from DLD-1 cells. The specific binding of HuR in the pull-down material was confirmed by western blotting. Biotinylated RNA from partial human reverse GAPDH (hrg) was used as a negative control, whereas 0.1 μ g of recombinant HuR (GST-HuR) instead of cell lysates was incubated with the same amount of biotinylated 5′UTR of caspase-2 and used as a positive control. Data shown are representative of two independent experiments giving similar results
    Horseradish Peroxidase Hrp Linked Goat Anti Rabbit Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp linked goat anti rabbit igg/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp linked goat anti rabbit igg - by Bioz Stars, 2020-02
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    78
    Santa Cruz Biotechnology bovine anti goat hrp linked igg
    Constitutive binding of <t>HuR</t> to the 5′UTR of Caspase-2 mRNA in DLD-1 cells. ( a ) An RNP-IP assay was performed in combination with RT-PCR from total cell lysates of DLD-1 cells to amplify mRNA species specifically bound by HuR. HuR-bound mRNA was precipitated by addition of a monoclonal HuR antibody (HuR) or, alternatively, the same amount of mouse <t>IgG</t> (IgG) as negative control. After elution from Sepharose beads, the isolated RNA samples were analyzed by RT-PCR using primer pairs, complementary and specific to the coding region of the indicated genes. The length of corresponding PCR products was determined by a DNA ladder. The specific immunoprecipitation (IP) of HuR is confirmed by western blot analysis (W.blot) shown in the right part of the panel. Data shown are representative of two independent experiments giving similar results. ( b , upper panel) Schematic representation of the different splice variants from human caspase-2 mRNA including caspase-2L (v1), caspase-2S (v2), and variant 3 (v3) encoding transcripts. The relative position of start (gray) and stop (dark) codons is depicted by triangles. Boxes show the relative position of exons and lacking exons are symbolized by a continuous line. The doubleheaded arrows indicate different regions (I, III and IV) encompassing variant-specific sequences (lines) or a region (II) commonly found in all three splice variants (dotted). ( b , lower panel) Crosslinking-RNP-IP analysis demonstrating that HuR specifically interacts with the 5′UTR of caspase-2L (v1) and splice variant 3, respectively. RNA-protein interactions were crosslinked with formaldehyde before HuR-bound mRNA was immunoprecipitated by addition of a HuR-specific antibody (HuR) or, alternatively, by the same amount of mouse IgG (IgG). The precipitated RNA samples were analyzed by RT-PCR by using primer pairs, spanning the indicated regions of caspase-2 mRNA (roman numerals). Input levels of different caspase-2 transcripts were isolated before the IP and were assessed by RT-PCR using the same primer pairs. ( c ) Biotin pull-down assay demonstrating a specific HuR binding to the 5′UTR of caspase-2. For pull-down assay, the biotinylated transcript encompassing 241 nucleotides of the 5′UTR of human caspase-2 (5′UTR Casp-2) was incubated with 300 μ g of total cell lysates from DLD-1 cells. The specific binding of HuR in the pull-down material was confirmed by western blotting. Biotinylated RNA from partial human reverse GAPDH (hrg) was used as a negative control, whereas 0.1 μ g of recombinant HuR (GST-HuR) instead of cell lysates was incubated with the same amount of biotinylated 5′UTR of caspase-2 and used as a positive control. Data shown are representative of two independent experiments giving similar results
    Bovine Anti Goat Hrp Linked Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine anti goat hrp linked igg/product/Santa Cruz Biotechnology
    Average 78 stars, based on 1 article reviews
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    bovine anti goat hrp linked igg - by Bioz Stars, 2020-02
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    99
    Santa Cruz Biotechnology hrp linked anti goat igg secondary antibody
    Constitutive binding of <t>HuR</t> to the 5′UTR of Caspase-2 mRNA in DLD-1 cells. ( a ) An RNP-IP assay was performed in combination with RT-PCR from total cell lysates of DLD-1 cells to amplify mRNA species specifically bound by HuR. HuR-bound mRNA was precipitated by addition of a monoclonal HuR antibody (HuR) or, alternatively, the same amount of mouse <t>IgG</t> (IgG) as negative control. After elution from Sepharose beads, the isolated RNA samples were analyzed by RT-PCR using primer pairs, complementary and specific to the coding region of the indicated genes. The length of corresponding PCR products was determined by a DNA ladder. The specific immunoprecipitation (IP) of HuR is confirmed by western blot analysis (W.blot) shown in the right part of the panel. Data shown are representative of two independent experiments giving similar results. ( b , upper panel) Schematic representation of the different splice variants from human caspase-2 mRNA including caspase-2L (v1), caspase-2S (v2), and variant 3 (v3) encoding transcripts. The relative position of start (gray) and stop (dark) codons is depicted by triangles. Boxes show the relative position of exons and lacking exons are symbolized by a continuous line. The doubleheaded arrows indicate different regions (I, III and IV) encompassing variant-specific sequences (lines) or a region (II) commonly found in all three splice variants (dotted). ( b , lower panel) Crosslinking-RNP-IP analysis demonstrating that HuR specifically interacts with the 5′UTR of caspase-2L (v1) and splice variant 3, respectively. RNA-protein interactions were crosslinked with formaldehyde before HuR-bound mRNA was immunoprecipitated by addition of a HuR-specific antibody (HuR) or, alternatively, by the same amount of mouse IgG (IgG). The precipitated RNA samples were analyzed by RT-PCR by using primer pairs, spanning the indicated regions of caspase-2 mRNA (roman numerals). Input levels of different caspase-2 transcripts were isolated before the IP and were assessed by RT-PCR using the same primer pairs. ( c ) Biotin pull-down assay demonstrating a specific HuR binding to the 5′UTR of caspase-2. For pull-down assay, the biotinylated transcript encompassing 241 nucleotides of the 5′UTR of human caspase-2 (5′UTR Casp-2) was incubated with 300 μ g of total cell lysates from DLD-1 cells. The specific binding of HuR in the pull-down material was confirmed by western blotting. Biotinylated RNA from partial human reverse GAPDH (hrg) was used as a negative control, whereas 0.1 μ g of recombinant HuR (GST-HuR) instead of cell lysates was incubated with the same amount of biotinylated 5′UTR of caspase-2 and used as a positive control. Data shown are representative of two independent experiments giving similar results
    Hrp Linked Anti Goat Igg Secondary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp linked anti goat igg secondary antibody/product/Santa Cruz Biotechnology
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp linked anti goat igg secondary antibody - by Bioz Stars, 2020-02
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    Constitutive binding of HuR to the 5′UTR of Caspase-2 mRNA in DLD-1 cells. ( a ) An RNP-IP assay was performed in combination with RT-PCR from total cell lysates of DLD-1 cells to amplify mRNA species specifically bound by HuR. HuR-bound mRNA was precipitated by addition of a monoclonal HuR antibody (HuR) or, alternatively, the same amount of mouse IgG (IgG) as negative control. After elution from Sepharose beads, the isolated RNA samples were analyzed by RT-PCR using primer pairs, complementary and specific to the coding region of the indicated genes. The length of corresponding PCR products was determined by a DNA ladder. The specific immunoprecipitation (IP) of HuR is confirmed by western blot analysis (W.blot) shown in the right part of the panel. Data shown are representative of two independent experiments giving similar results. ( b , upper panel) Schematic representation of the different splice variants from human caspase-2 mRNA including caspase-2L (v1), caspase-2S (v2), and variant 3 (v3) encoding transcripts. The relative position of start (gray) and stop (dark) codons is depicted by triangles. Boxes show the relative position of exons and lacking exons are symbolized by a continuous line. The doubleheaded arrows indicate different regions (I, III and IV) encompassing variant-specific sequences (lines) or a region (II) commonly found in all three splice variants (dotted). ( b , lower panel) Crosslinking-RNP-IP analysis demonstrating that HuR specifically interacts with the 5′UTR of caspase-2L (v1) and splice variant 3, respectively. RNA-protein interactions were crosslinked with formaldehyde before HuR-bound mRNA was immunoprecipitated by addition of a HuR-specific antibody (HuR) or, alternatively, by the same amount of mouse IgG (IgG). The precipitated RNA samples were analyzed by RT-PCR by using primer pairs, spanning the indicated regions of caspase-2 mRNA (roman numerals). Input levels of different caspase-2 transcripts were isolated before the IP and were assessed by RT-PCR using the same primer pairs. ( c ) Biotin pull-down assay demonstrating a specific HuR binding to the 5′UTR of caspase-2. For pull-down assay, the biotinylated transcript encompassing 241 nucleotides of the 5′UTR of human caspase-2 (5′UTR Casp-2) was incubated with 300 μ g of total cell lysates from DLD-1 cells. The specific binding of HuR in the pull-down material was confirmed by western blotting. Biotinylated RNA from partial human reverse GAPDH (hrg) was used as a negative control, whereas 0.1 μ g of recombinant HuR (GST-HuR) instead of cell lysates was incubated with the same amount of biotinylated 5′UTR of caspase-2 and used as a positive control. Data shown are representative of two independent experiments giving similar results

    Journal: Cell Death & Disease

    Article Title: Attenuation of the ELAV1-like protein HuR sensitizes adenocarcinoma cells to the intrinsic apoptotic pathway by increasing the translation of caspase-2L

    doi: 10.1038/cddis.2014.279

    Figure Lengend Snippet: Constitutive binding of HuR to the 5′UTR of Caspase-2 mRNA in DLD-1 cells. ( a ) An RNP-IP assay was performed in combination with RT-PCR from total cell lysates of DLD-1 cells to amplify mRNA species specifically bound by HuR. HuR-bound mRNA was precipitated by addition of a monoclonal HuR antibody (HuR) or, alternatively, the same amount of mouse IgG (IgG) as negative control. After elution from Sepharose beads, the isolated RNA samples were analyzed by RT-PCR using primer pairs, complementary and specific to the coding region of the indicated genes. The length of corresponding PCR products was determined by a DNA ladder. The specific immunoprecipitation (IP) of HuR is confirmed by western blot analysis (W.blot) shown in the right part of the panel. Data shown are representative of two independent experiments giving similar results. ( b , upper panel) Schematic representation of the different splice variants from human caspase-2 mRNA including caspase-2L (v1), caspase-2S (v2), and variant 3 (v3) encoding transcripts. The relative position of start (gray) and stop (dark) codons is depicted by triangles. Boxes show the relative position of exons and lacking exons are symbolized by a continuous line. The doubleheaded arrows indicate different regions (I, III and IV) encompassing variant-specific sequences (lines) or a region (II) commonly found in all three splice variants (dotted). ( b , lower panel) Crosslinking-RNP-IP analysis demonstrating that HuR specifically interacts with the 5′UTR of caspase-2L (v1) and splice variant 3, respectively. RNA-protein interactions were crosslinked with formaldehyde before HuR-bound mRNA was immunoprecipitated by addition of a HuR-specific antibody (HuR) or, alternatively, by the same amount of mouse IgG (IgG). The precipitated RNA samples were analyzed by RT-PCR by using primer pairs, spanning the indicated regions of caspase-2 mRNA (roman numerals). Input levels of different caspase-2 transcripts were isolated before the IP and were assessed by RT-PCR using the same primer pairs. ( c ) Biotin pull-down assay demonstrating a specific HuR binding to the 5′UTR of caspase-2. For pull-down assay, the biotinylated transcript encompassing 241 nucleotides of the 5′UTR of human caspase-2 (5′UTR Casp-2) was incubated with 300 μ g of total cell lysates from DLD-1 cells. The specific binding of HuR in the pull-down material was confirmed by western blotting. Biotinylated RNA from partial human reverse GAPDH (hrg) was used as a negative control, whereas 0.1 μ g of recombinant HuR (GST-HuR) instead of cell lysates was incubated with the same amount of biotinylated 5′UTR of caspase-2 and used as a positive control. Data shown are representative of two independent experiments giving similar results

    Article Snippet: Antibodies raised against HuR, anti-rabbit, anti-mouse and anti-goat horseradish peroxidase linked IgG were purchased from Santa Cruz Biotechnology (Heidelberg, Germany).

    Techniques: Binding Assay, Reverse Transcription Polymerase Chain Reaction, Negative Control, Isolation, Polymerase Chain Reaction, Immunoprecipitation, Western Blot, Variant Assay, Pull Down Assay, Incubation, Recombinant, Positive Control