horseradish peroxidase hrp streptavidin  (Thermo Fisher)


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    Thermo Fisher horseradish peroxidase hrp streptavidin
    Horseradish Peroxidase Hrp Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp streptavidin/product/Thermo Fisher
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp streptavidin - by Bioz Stars, 2020-03
    94/100 stars

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    Related Articles

    Chemiluminescent ELISA:

    Article Title: An α-Gal-containing neoglycoprotein-based vaccine partially protects against murine cutaneous leishmaniasis caused by Leishmania major
    Article Snippet: Paragraph title: Chemiluminescent enzyme-linked immunosorbent assay (chemiluminescent ELISA) with CL-infected patients ... Horseradish peroxidase (HRP)-streptavidin (Invitrogen; 50 μL, 1:2000 dilution) in PBS was added and plates were incubated for 30 min at 37°C.

    Reporter Assay:

    Article Title: Bacterial Lipopolysaccharide Destabilizes Influenza Viruses
    Article Snippet: To test whether LPS binds to virions, we used a modified version of a solid-phase influenza receptor binding assay described previously ( ). .. After the wells were washed, dilutions of biotinylated E. coli O111:B4 LPS (InvivoGen) were added and incubated at 37°C for 1 h. LPS binding was detected using horseradish peroxidase (HRP)-streptavidin (Thermo Fisher), 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (Thermo Fisher), and 0.2 M sulfuric acid.

    Blocking Assay:

    Article Title: An α-Gal-containing neoglycoprotein-based vaccine partially protects against murine cutaneous leishmaniasis caused by Leishmania major
    Article Snippet: Horseradish peroxidase (HRP)-streptavidin (Invitrogen; 50 μL, 1:2000 dilution) in PBS was added and plates were incubated for 30 min at 37°C. .. Plates were washed three times between steps with PBS-T, except before blocking.

    Article Title: Inhibitory Effect of Silibinin against Azoxymethane-induced Colon Tumorigenesis in A/J Mice
    Article Snippet: Paraffin-embedded sections (5 μm) were subjected to antigen retrieval and blocking of endogenous peroxidase activity ( ). .. Sections were then incubated with biotinylated appropriate secondary antibody for 1 hr at room temperature followed by 30 min incubation with conjugated horseradish peroxidase (HRP)-streptavidin (Invitrogen) and then incubated with DAB (Vector Laboratories, Inc.) working solution at room temperature followed by counterstaining with diluted Harris hematoxylin (Sigma) and mounted.

    Article Title: Timing and expression of the angiopoietin-1–Tie-2 pathway in murine lung development and congenital diaphragmatic hernia
    Article Snippet: Sections were incubated in universal CAS Block (Zymed, Carlsbad, CA) for 1 hour at room temperature prior to the application of primary antibodies. .. For chromogenic development, sections were then incubated in horseradish peroxidase (HRP)-streptavidin (Zymed) for 30 minutes, developed with either Nova Red (Vector) or AEC solution (Invitrogen), and counterstained in hematoxylin.

    Enzyme-linked Immunosorbent Assay:

    Article Title: PDZ Protein Interactions Underlying NMDA Receptor-Mediated Excitotoxicity and Neuroprotection by PSD-95 Inhibitors
    Article Snippet: Paragraph title: ELISA-based PDZ domain interaction assays. ... Horseradish peroxidase (HRP) streptavidin (1:2000 dilution in 2% BSA; Zymed, South San Francisco CA) was added to each well and incubated on ice for 20 min. After five washes with PBS, TMB substrate was added (Dako, Milano, Italy).

    Microarray:

    Article Title: Assessment of folate receptor alpha and beta expression in selection of lung and pancreatic cancer patients for receptor targeted therapies
    Article Snippet: Horseradish peroxidase (HRP)-streptavidin and EZ-Link Sulfo-NHS-LC-Biotin were purchased from Thermo Scientific (Madison, WI). .. The cancer tissue microarrays examined consisted of a pancreatic cancer microarray from the Translational Genomics Research Institute (Phoenix, AZ), a custom multi-tumor tissue microarray (TMA-00300) from Asterand (Detroit, MI) and a lung cancer microarray from University of Pennsylvania (Philadelphia, PA).

    Incubation:

    Article Title: An α-Gal-containing neoglycoprotein-based vaccine partially protects against murine cutaneous leishmaniasis caused by Leishmania major
    Article Snippet: .. Horseradish peroxidase (HRP)-streptavidin (Invitrogen; 50 μL, 1:2000 dilution) in PBS was added and plates were incubated for 30 min at 37°C. .. Plates were washed three times between steps with PBS-T, except before blocking.

    Article Title: PDZ Protein Interactions Underlying NMDA Receptor-Mediated Excitotoxicity and Neuroprotection by PSD-95 Inhibitors
    Article Snippet: .. Horseradish peroxidase (HRP) streptavidin (1:2000 dilution in 2% BSA; Zymed, South San Francisco CA) was added to each well and incubated on ice for 20 min. After five washes with PBS, TMB substrate was added (Dako, Milano, Italy). .. Plates were incubated at room temperature in the dark for 30 min, after which time 0.18 m H2 SO4 was added to stop the reaction.

    Article Title: Inhibitory Effect of Silibinin against Azoxymethane-induced Colon Tumorigenesis in A/J Mice
    Article Snippet: .. Sections were then incubated with biotinylated appropriate secondary antibody for 1 hr at room temperature followed by 30 min incubation with conjugated horseradish peroxidase (HRP)-streptavidin (Invitrogen) and then incubated with DAB (Vector Laboratories, Inc.) working solution at room temperature followed by counterstaining with diluted Harris hematoxylin (Sigma) and mounted. .. Microscopic IHC analyses were performed using Zeiss Axioscop 2 microscope (Carl Zeiss); photomicrographs captured by Carl Zeiss AxioCam MrC5 camera with Axiovision Rel 4.5 software.

    Article Title: Mucin acts as a nutrient source and a signal for the differential expression of genes coding for cellular processes and virulence factors in Acinetobacter baumannii
    Article Snippet: .. The plates were then washed with PBS and incubated with a 1:1,500 dilution of horseradish peroxidase (HRP)-streptavidin (Life Technologies) for 1 h at 4°C. ..

    Article Title: Timing and expression of the angiopoietin-1–Tie-2 pathway in murine lung development and congenital diaphragmatic hernia
    Article Snippet: .. For chromogenic development, sections were then incubated in horseradish peroxidase (HRP)-streptavidin (Zymed) for 30 minutes, developed with either Nova Red (Vector) or AEC solution (Invitrogen), and counterstained in hematoxylin. .. For fluorescent multilabeling, conjugates of streptavidin with Alexa Fluor 555 and Alexa Fluor 488 were used (1:200; Invitrogen) along with a Hoechst 33342 nuclear counterstain.

    Article Title: Bacterial Lipopolysaccharide Destabilizes Influenza Viruses
    Article Snippet: .. After the wells were washed, dilutions of biotinylated E. coli O111:B4 LPS (InvivoGen) were added and incubated at 37°C for 1 h. LPS binding was detected using horseradish peroxidase (HRP)-streptavidin (Thermo Fisher), 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (Thermo Fisher), and 0.2 M sulfuric acid. ..

    Article Title: Plant-Expressed Recombinant Mountain Cedar Allergen Jun a 1 Is Allergenic and Has Limited Pectate Lyase Activity
    Article Snippet: The blot was blocked with TBST buffer (100 m M Tris-HCl, 0.9% NaCl, 0.1% Tween 20, pH 7.5) containing 10% BSA at room temperature for 1 h. The membranes were then incubated, at 4°C over night, with a mixture of 0.5 μg/ml each of anti-Jun a 1 monoclonal antibodies (L1b-18, L86-10, L27b-21, L20-15, and KW-S91 anti-Cry j 1 antibody; KW-S91 was a gift of M. Watanabe, PhD, New Drug Research Laboratories II, Kowa Co. Ltd., Tokyo, Japan). .. Bound mouse IgG was detected by biotinylated goat anti-mouse IgG and horseradish peroxidase (HRP)-streptavidin (Zymed, San Francisco, Calif., USA) [ ].

    Article Title: Sertoli Cell-Specific Deletion of the Androgen Receptor Compromises Testicular Immune Privilege in Mice 1
    Article Snippet: .. After several washes, the sections were incubated with a 1:200 dilution of biotin-goat-anti-rat IgG (Zymed Laboratories-Invitrogen) for 30 min at 25°C, washed in several changes of PBS, incubated with a 1:100 dilution of horseradish peroxidase (HRP)-streptavidin (Zymed Laboratories) in PBS containing 3% normal goat serum for 20 min at 25°C, and washed in four changes of PBS. .. The sections were stained with aminoethyl carbazole (Zymed Laboratories), washed in water for 10 min, counterstained with hematoxylin, rinsed with water for 10 min, and mounted with GVA mounting medium (Invitrogen).

    Immunocytochemistry:

    Article Title: Sertoli Cell-Specific Deletion of the Androgen Receptor Compromises Testicular Immune Privilege in Mice 1
    Article Snippet: Paragraph title: Immunofluorescence and Immunocytochemistry ... After several washes, the sections were incubated with a 1:200 dilution of biotin-goat-anti-rat IgG (Zymed Laboratories-Invitrogen) for 30 min at 25°C, washed in several changes of PBS, incubated with a 1:100 dilution of horseradish peroxidase (HRP)-streptavidin (Zymed Laboratories) in PBS containing 3% normal goat serum for 20 min at 25°C, and washed in four changes of PBS.

    Activity Assay:

    Article Title: Inhibitory Effect of Silibinin against Azoxymethane-induced Colon Tumorigenesis in A/J Mice
    Article Snippet: Paraffin-embedded sections (5 μm) were subjected to antigen retrieval and blocking of endogenous peroxidase activity ( ). .. Sections were then incubated with biotinylated appropriate secondary antibody for 1 hr at room temperature followed by 30 min incubation with conjugated horseradish peroxidase (HRP)-streptavidin (Invitrogen) and then incubated with DAB (Vector Laboratories, Inc.) working solution at room temperature followed by counterstaining with diluted Harris hematoxylin (Sigma) and mounted.

    Article Title: Timing and expression of the angiopoietin-1–Tie-2 pathway in murine lung development and congenital diaphragmatic hernia
    Article Snippet: Endogenous peroxidase activity was quenched in 0.3% hydrogen peroxide in methanol for 20 minutes. .. For chromogenic development, sections were then incubated in horseradish peroxidase (HRP)-streptavidin (Zymed) for 30 minutes, developed with either Nova Red (Vector) or AEC solution (Invitrogen), and counterstained in hematoxylin.

    Modification:

    Article Title: Bacterial Lipopolysaccharide Destabilizes Influenza Viruses
    Article Snippet: To test whether LPS binds to virions, we used a modified version of a solid-phase influenza receptor binding assay described previously ( ). .. After the wells were washed, dilutions of biotinylated E. coli O111:B4 LPS (InvivoGen) were added and incubated at 37°C for 1 h. LPS binding was detected using horseradish peroxidase (HRP)-streptavidin (Thermo Fisher), 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (Thermo Fisher), and 0.2 M sulfuric acid.

    Immunohistochemistry:

    Article Title: Inhibitory Effect of Silibinin against Azoxymethane-induced Colon Tumorigenesis in A/J Mice
    Article Snippet: Paragraph title: IHC analyses ... Sections were then incubated with biotinylated appropriate secondary antibody for 1 hr at room temperature followed by 30 min incubation with conjugated horseradish peroxidase (HRP)-streptavidin (Invitrogen) and then incubated with DAB (Vector Laboratories, Inc.) working solution at room temperature followed by counterstaining with diluted Harris hematoxylin (Sigma) and mounted.

    Article Title: Timing and expression of the angiopoietin-1–Tie-2 pathway in murine lung development and congenital diaphragmatic hernia
    Article Snippet: Paragraph title: Immunohistochemistry ... For chromogenic development, sections were then incubated in horseradish peroxidase (HRP)-streptavidin (Zymed) for 30 minutes, developed with either Nova Red (Vector) or AEC solution (Invitrogen), and counterstained in hematoxylin.

    Article Title: Sertoli Cell-Specific Deletion of the Androgen Receptor Compromises Testicular Immune Privilege in Mice 1
    Article Snippet: For immunohistochemical detection of macrophages, the sections were incubated with 3% normal goat serum in PBS for 1 h and then incubated overnight at 4°C in a moist chamber with a 1:100 dilution of rat anti-F4/80 antibody (eBioscience) in PBS containing 3% normal goat serum. .. After several washes, the sections were incubated with a 1:200 dilution of biotin-goat-anti-rat IgG (Zymed Laboratories-Invitrogen) for 30 min at 25°C, washed in several changes of PBS, incubated with a 1:100 dilution of horseradish peroxidase (HRP)-streptavidin (Zymed Laboratories) in PBS containing 3% normal goat serum for 20 min at 25°C, and washed in four changes of PBS.

    Imaging:

    Article Title: Timing and expression of the angiopoietin-1–Tie-2 pathway in murine lung development and congenital diaphragmatic hernia
    Article Snippet: For chromogenic development, sections were then incubated in horseradish peroxidase (HRP)-streptavidin (Zymed) for 30 minutes, developed with either Nova Red (Vector) or AEC solution (Invitrogen), and counterstained in hematoxylin. .. All microscopy imaging was performed using a Nikon Eclipse E600 apparatus.

    Negative Staining:

    Article Title: Inhibitory Effect of Silibinin against Azoxymethane-induced Colon Tumorigenesis in A/J Mice
    Article Snippet: In all IHC, negative staining controls were used where sections were incubated with N-Universal Negative Control mouse or rabbit antibody (DAKO) under identical conditions. .. Sections were then incubated with biotinylated appropriate secondary antibody for 1 hr at room temperature followed by 30 min incubation with conjugated horseradish peroxidase (HRP)-streptavidin (Invitrogen) and then incubated with DAB (Vector Laboratories, Inc.) working solution at room temperature followed by counterstaining with diluted Harris hematoxylin (Sigma) and mounted.

    Immunofluorescence:

    Article Title: Sertoli Cell-Specific Deletion of the Androgen Receptor Compromises Testicular Immune Privilege in Mice 1
    Article Snippet: Paragraph title: Immunofluorescence and Immunocytochemistry ... After several washes, the sections were incubated with a 1:200 dilution of biotin-goat-anti-rat IgG (Zymed Laboratories-Invitrogen) for 30 min at 25°C, washed in several changes of PBS, incubated with a 1:100 dilution of horseradish peroxidase (HRP)-streptavidin (Zymed Laboratories) in PBS containing 3% normal goat serum for 20 min at 25°C, and washed in four changes of PBS.

    Mutagenesis:

    Article Title: Sertoli Cell-Specific Deletion of the Androgen Receptor Compromises Testicular Immune Privilege in Mice 1
    Article Snippet: To determine the location of CD11B (ITGAM)(+) leukocytes, testis sections from WT and S- Ar mutant mice were deparaffinized, blocked 1 h in 3% goat serum in PBS-T, and incubated 1 h with a 1:100 dilution of anti-CD11B-CY3 antibody (Jackson Laboratory). .. After several washes, the sections were incubated with a 1:200 dilution of biotin-goat-anti-rat IgG (Zymed Laboratories-Invitrogen) for 30 min at 25°C, washed in several changes of PBS, incubated with a 1:100 dilution of horseradish peroxidase (HRP)-streptavidin (Zymed Laboratories) in PBS containing 3% normal goat serum for 20 min at 25°C, and washed in four changes of PBS.

    Bicinchoninic Acid Protein Assay:

    Article Title: Antiapoptotic Effect and Inhibition of Ischemia/Reperfusion-Induced Myocardial Injury in Metallothionein-Overexpressing Transgenic Mice
    Article Snippet: Biotinylated goat anti-rabbit IgG antibody and horseradish peroxidase (HRP)-streptavidin were obtained from Zymed Laboratories, Inc. (San Francisco, CA). .. A bicinchoninic acid protein assay kit was the product of Pierce (Rockford, IL).

    Avidin-Biotin Assay:

    Article Title: Timing and expression of the angiopoietin-1–Tie-2 pathway in murine lung development and congenital diaphragmatic hernia
    Article Snippet: Endogenous biotin was reduced via an avidin-biotin blocking kit (Vector, Burlingame, CA). .. For chromogenic development, sections were then incubated in horseradish peroxidase (HRP)-streptavidin (Zymed) for 30 minutes, developed with either Nova Red (Vector) or AEC solution (Invitrogen), and counterstained in hematoxylin.

    Microscopy:

    Article Title: Inhibitory Effect of Silibinin against Azoxymethane-induced Colon Tumorigenesis in A/J Mice
    Article Snippet: Sections were then incubated with biotinylated appropriate secondary antibody for 1 hr at room temperature followed by 30 min incubation with conjugated horseradish peroxidase (HRP)-streptavidin (Invitrogen) and then incubated with DAB (Vector Laboratories, Inc.) working solution at room temperature followed by counterstaining with diluted Harris hematoxylin (Sigma) and mounted. .. Microscopic IHC analyses were performed using Zeiss Axioscop 2 microscope (Carl Zeiss); photomicrographs captured by Carl Zeiss AxioCam MrC5 camera with Axiovision Rel 4.5 software.

    Article Title: Timing and expression of the angiopoietin-1–Tie-2 pathway in murine lung development and congenital diaphragmatic hernia
    Article Snippet: For chromogenic development, sections were then incubated in horseradish peroxidase (HRP)-streptavidin (Zymed) for 30 minutes, developed with either Nova Red (Vector) or AEC solution (Invitrogen), and counterstained in hematoxylin. .. All microscopy imaging was performed using a Nikon Eclipse E600 apparatus.

    Mouse Assay:

    Article Title: Sertoli Cell-Specific Deletion of the Androgen Receptor Compromises Testicular Immune Privilege in Mice 1
    Article Snippet: To determine the location of CD11B (ITGAM)(+) leukocytes, testis sections from WT and S- Ar mutant mice were deparaffinized, blocked 1 h in 3% goat serum in PBS-T, and incubated 1 h with a 1:100 dilution of anti-CD11B-CY3 antibody (Jackson Laboratory). .. After several washes, the sections were incubated with a 1:200 dilution of biotin-goat-anti-rat IgG (Zymed Laboratories-Invitrogen) for 30 min at 25°C, washed in several changes of PBS, incubated with a 1:100 dilution of horseradish peroxidase (HRP)-streptavidin (Zymed Laboratories) in PBS containing 3% normal goat serum for 20 min at 25°C, and washed in four changes of PBS.

    Labeling:

    Article Title: Mucin acts as a nutrient source and a signal for the differential expression of genes coding for cellular processes and virulence factors in Acinetobacter baumannii
    Article Snippet: Final concentrations of biotinylated mucin used in either the protein or carbohydrate degradation assays were obtained based on a validation assay to determine which concentrations of each type of labeled mucin gave relative luminescence units (RLUs) that were comparable to one another. .. The plates were then washed with PBS and incubated with a 1:1,500 dilution of horseradish peroxidase (HRP)-streptavidin (Life Technologies) for 1 h at 4°C.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Plant-Expressed Recombinant Mountain Cedar Allergen Jun a 1 Is Allergenic and Has Limited Pectate Lyase Activity
    Article Snippet: Total plant extracts of Jun a 1 and purified rJun a 1 were separated on 4–20% Tris-HCl PAGE gels (Bio-Rad) and subsequently stained with Coomassie blue R-250. .. Bound mouse IgG was detected by biotinylated goat anti-mouse IgG and horseradish peroxidase (HRP)-streptavidin (Zymed, San Francisco, Calif., USA) [ ].

    Purification:

    Article Title: Plant-Expressed Recombinant Mountain Cedar Allergen Jun a 1 Is Allergenic and Has Limited Pectate Lyase Activity
    Article Snippet: Total plant extracts of Jun a 1 and purified rJun a 1 were separated on 4–20% Tris-HCl PAGE gels (Bio-Rad) and subsequently stained with Coomassie blue R-250. .. Bound mouse IgG was detected by biotinylated goat anti-mouse IgG and horseradish peroxidase (HRP)-streptavidin (Zymed, San Francisco, Calif., USA) [ ].

    SDS Page:

    Article Title: Plant-Expressed Recombinant Mountain Cedar Allergen Jun a 1 Is Allergenic and Has Limited Pectate Lyase Activity
    Article Snippet: Paragraph title: SDS-PAGE and Immunoblotting of rJun a 1 ... Bound mouse IgG was detected by biotinylated goat anti-mouse IgG and horseradish peroxidase (HRP)-streptavidin (Zymed, San Francisco, Calif., USA) [ ].

    Plasmid Preparation:

    Article Title: Timing and expression of the angiopoietin-1–Tie-2 pathway in murine lung development and congenital diaphragmatic hernia
    Article Snippet: .. For chromogenic development, sections were then incubated in horseradish peroxidase (HRP)-streptavidin (Zymed) for 30 minutes, developed with either Nova Red (Vector) or AEC solution (Invitrogen), and counterstained in hematoxylin. .. For fluorescent multilabeling, conjugates of streptavidin with Alexa Fluor 555 and Alexa Fluor 488 were used (1:200; Invitrogen) along with a Hoechst 33342 nuclear counterstain.

    Software:

    Article Title: Inhibitory Effect of Silibinin against Azoxymethane-induced Colon Tumorigenesis in A/J Mice
    Article Snippet: Sections were then incubated with biotinylated appropriate secondary antibody for 1 hr at room temperature followed by 30 min incubation with conjugated horseradish peroxidase (HRP)-streptavidin (Invitrogen) and then incubated with DAB (Vector Laboratories, Inc.) working solution at room temperature followed by counterstaining with diluted Harris hematoxylin (Sigma) and mounted. .. Microscopic IHC analyses were performed using Zeiss Axioscop 2 microscope (Carl Zeiss); photomicrographs captured by Carl Zeiss AxioCam MrC5 camera with Axiovision Rel 4.5 software.

    Negative Control:

    Article Title: Inhibitory Effect of Silibinin against Azoxymethane-induced Colon Tumorigenesis in A/J Mice
    Article Snippet: In all IHC, negative staining controls were used where sections were incubated with N-Universal Negative Control mouse or rabbit antibody (DAKO) under identical conditions. .. Sections were then incubated with biotinylated appropriate secondary antibody for 1 hr at room temperature followed by 30 min incubation with conjugated horseradish peroxidase (HRP)-streptavidin (Invitrogen) and then incubated with DAB (Vector Laboratories, Inc.) working solution at room temperature followed by counterstaining with diluted Harris hematoxylin (Sigma) and mounted.

    Binding Assay:

    Article Title: PDZ Protein Interactions Underlying NMDA Receptor-Mediated Excitotoxicity and Neuroprotection by PSD-95 Inhibitors
    Article Snippet: In experiments aimed at EC50 determinations, test peptides were generally applied at concentrations ranging from 0.001 to 100 μ m after titrating the amount of GST-PDZ protein on the plate to allow binding saturation below the absorbance limit of the microplate reader. .. Horseradish peroxidase (HRP) streptavidin (1:2000 dilution in 2% BSA; Zymed, South San Francisco CA) was added to each well and incubated on ice for 20 min. After five washes with PBS, TMB substrate was added (Dako, Milano, Italy).

    Article Title: Bacterial Lipopolysaccharide Destabilizes Influenza Viruses
    Article Snippet: .. After the wells were washed, dilutions of biotinylated E. coli O111:B4 LPS (InvivoGen) were added and incubated at 37°C for 1 h. LPS binding was detected using horseradish peroxidase (HRP)-streptavidin (Thermo Fisher), 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (Thermo Fisher), and 0.2 M sulfuric acid. ..

    In Situ:

    Article Title: Antiapoptotic Effect and Inhibition of Ischemia/Reperfusion-Induced Myocardial Injury in Metallothionein-Overexpressing Transgenic Mice
    Article Snippet: ApopTag in situ apoptosis detection kit was purchased from Intergen (Purchase, NY). .. Biotinylated goat anti-rabbit IgG antibody and horseradish peroxidase (HRP)-streptavidin were obtained from Zymed Laboratories, Inc. (San Francisco, CA).

    Staining:

    Article Title: Inhibitory Effect of Silibinin against Azoxymethane-induced Colon Tumorigenesis in A/J Mice
    Article Snippet: Sections were then incubated with biotinylated appropriate secondary antibody for 1 hr at room temperature followed by 30 min incubation with conjugated horseradish peroxidase (HRP)-streptavidin (Invitrogen) and then incubated with DAB (Vector Laboratories, Inc.) working solution at room temperature followed by counterstaining with diluted Harris hematoxylin (Sigma) and mounted. .. Positive cells for various IHC stained molecules were quantified by counting brown-stained cells among total number of cells at 5 randomly selected fields at x400 magnification.

    Article Title: Plant-Expressed Recombinant Mountain Cedar Allergen Jun a 1 Is Allergenic and Has Limited Pectate Lyase Activity
    Article Snippet: Total plant extracts of Jun a 1 and purified rJun a 1 were separated on 4–20% Tris-HCl PAGE gels (Bio-Rad) and subsequently stained with Coomassie blue R-250. .. Bound mouse IgG was detected by biotinylated goat anti-mouse IgG and horseradish peroxidase (HRP)-streptavidin (Zymed, San Francisco, Calif., USA) [ ].

    Article Title: Sertoli Cell-Specific Deletion of the Androgen Receptor Compromises Testicular Immune Privilege in Mice 1
    Article Snippet: After several washes, the sections were incubated with a 1:200 dilution of biotin-goat-anti-rat IgG (Zymed Laboratories-Invitrogen) for 30 min at 25°C, washed in several changes of PBS, incubated with a 1:100 dilution of horseradish peroxidase (HRP)-streptavidin (Zymed Laboratories) in PBS containing 3% normal goat serum for 20 min at 25°C, and washed in four changes of PBS. .. The sections were stained with aminoethyl carbazole (Zymed Laboratories), washed in water for 10 min, counterstained with hematoxylin, rinsed with water for 10 min, and mounted with GVA mounting medium (Invitrogen).

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  • 99
    Thermo Fisher streptavidin hrp
    Schematic diagram showed non-radioactive metabolic incorporation followed by azide-biotin or azide-Alex555 labeling, and biotin signals of proteins were detected by <t>streptavidin-HRP</t> by western blot. HPG is incorporated into newly synthesized proteins by metabolism and protein synthesis and the triazole conjugation between newly alkyne proteins labeled HPG and azide labeled either biotin or Alex555 via CuSO4 catalysis (A) . (B-a) The detection of biotin signals from extracted total proteins labeled by labeling reaction. Normal culture medium was changed to replace DMEM free of L-methionine supplemented with HPG after pulse 4 hr, and proteins were extracted in each of group at various time points including 0, 4, 24 and 72 hr. (B-b) Biotin signals of total proteins were detected. 1: Normal culture condition group; 2: HPG plus anisomycin group; 3: HPG group. (B-c,d,e) Biotin signals of Bcl-2, MMP-9 and IgG were individually detected in the immunoprecipitate pulled down by primary antibodies via siRNA post-transfection followed by non-radioactive metabolic labeling. (B-f) Radioactive isotope 35 S-methonine incorporated into synthesized IgG purified by immunoprecipitation was detected by autoradiography. 1: 35 S-methonine treated human choriocarcinoma cell line BeWo group and then antibody against human IgG immunoprecipitated human IgG in extracted total proteins; 2: cycloheximide plus 35 S-methonine treated BeWo group then antibody against human IgG immunoprecipitated human IgG in extracted total proteins; 3: 35 S-methonine treated skin fibroblast and then antibody against human IgG immunoprecipitated human IgG in extracted total proteins.
    Streptavidin Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin hrp/product/Thermo Fisher
    Average 99 stars, based on 162 article reviews
    Price from $9.99 to $1999.99
    streptavidin hrp - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher streptavidin conjugated hrp
    Photoaffinity labeling of various PrP species. <t>Streptavidin-HRP-probed</t> blots of samples photoaffinity labeled with PA-PBD peptide. (A) Samples containing PrP Int1 or PrP C were incubated with or without PA-PBD and exposed to UV light for varying time periods, as indicated. (B) Samples containing α -helical PrP or PrP Int1 were incubated with PA-PBD and exposed to UV light for 5 min. (C) Samples of PrP Int1 were incubated with varying concentrations of PA-PBD, as indicated, and exposed to UV light for 0 or 5 min, as indicated. (D) Sample containing 7 μ g of PrP Int1 photoaffinity labeled with PA-PBD (PA-PrP Int1 ) is compared to a standard curve of biotinylated AviTag PrP for reference.
    Streptavidin Conjugated Hrp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin conjugated hrp/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    streptavidin conjugated hrp - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

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    Schematic diagram showed non-radioactive metabolic incorporation followed by azide-biotin or azide-Alex555 labeling, and biotin signals of proteins were detected by streptavidin-HRP by western blot. HPG is incorporated into newly synthesized proteins by metabolism and protein synthesis and the triazole conjugation between newly alkyne proteins labeled HPG and azide labeled either biotin or Alex555 via CuSO4 catalysis (A) . (B-a) The detection of biotin signals from extracted total proteins labeled by labeling reaction. Normal culture medium was changed to replace DMEM free of L-methionine supplemented with HPG after pulse 4 hr, and proteins were extracted in each of group at various time points including 0, 4, 24 and 72 hr. (B-b) Biotin signals of total proteins were detected. 1: Normal culture condition group; 2: HPG plus anisomycin group; 3: HPG group. (B-c,d,e) Biotin signals of Bcl-2, MMP-9 and IgG were individually detected in the immunoprecipitate pulled down by primary antibodies via siRNA post-transfection followed by non-radioactive metabolic labeling. (B-f) Radioactive isotope 35 S-methonine incorporated into synthesized IgG purified by immunoprecipitation was detected by autoradiography. 1: 35 S-methonine treated human choriocarcinoma cell line BeWo group and then antibody against human IgG immunoprecipitated human IgG in extracted total proteins; 2: cycloheximide plus 35 S-methonine treated BeWo group then antibody against human IgG immunoprecipitated human IgG in extracted total proteins; 3: 35 S-methonine treated skin fibroblast and then antibody against human IgG immunoprecipitated human IgG in extracted total proteins.

    Journal: BMC Cell Biology

    Article Title: A morphologic and semi-quantitative technique to analyze synthesis and release of specific proteins in cells

    doi: 10.1186/s12860-014-0045-1

    Figure Lengend Snippet: Schematic diagram showed non-radioactive metabolic incorporation followed by azide-biotin or azide-Alex555 labeling, and biotin signals of proteins were detected by streptavidin-HRP by western blot. HPG is incorporated into newly synthesized proteins by metabolism and protein synthesis and the triazole conjugation between newly alkyne proteins labeled HPG and azide labeled either biotin or Alex555 via CuSO4 catalysis (A) . (B-a) The detection of biotin signals from extracted total proteins labeled by labeling reaction. Normal culture medium was changed to replace DMEM free of L-methionine supplemented with HPG after pulse 4 hr, and proteins were extracted in each of group at various time points including 0, 4, 24 and 72 hr. (B-b) Biotin signals of total proteins were detected. 1: Normal culture condition group; 2: HPG plus anisomycin group; 3: HPG group. (B-c,d,e) Biotin signals of Bcl-2, MMP-9 and IgG were individually detected in the immunoprecipitate pulled down by primary antibodies via siRNA post-transfection followed by non-radioactive metabolic labeling. (B-f) Radioactive isotope 35 S-methonine incorporated into synthesized IgG purified by immunoprecipitation was detected by autoradiography. 1: 35 S-methonine treated human choriocarcinoma cell line BeWo group and then antibody against human IgG immunoprecipitated human IgG in extracted total proteins; 2: cycloheximide plus 35 S-methonine treated BeWo group then antibody against human IgG immunoprecipitated human IgG in extracted total proteins; 3: 35 S-methonine treated skin fibroblast and then antibody against human IgG immunoprecipitated human IgG in extracted total proteins.

    Article Snippet: Each diluted standard was incubated with primary antibody coated on the well and streptavidin-HRP and OD value for each sample was detected by ELISA.

    Techniques: Labeling, Western Blot, Synthesized, Conjugation Assay, Transfection, Purification, Immunoprecipitation, Autoradiography

    Electron microscopy of PA1b-bound V-ATPase. A , representative classes of PA1b-streptavidin-HRP-bound V-ATPase in the absence of ATP. B , as A but in the presence of 2 m m Mg·ATP. The PA1b-streptavidin-HRP density is indicated by an arrow in the far left panel 1 of A. Scale bars in both A and B represent 15 nm. C–E , three-dimensional reconstructions of the V-ATPase viewed perpendicular to the long axis of the complex ( upper image ) and from the extracellular end ( lower image ) bound to PA1b ( C ), bound to PA1b after the addition of Mg·ATP ( D ) and a control with no PA1b ( E ). All models were generated using EMAN, and the picture was produced using Chimera rendered at the same sigma level. In C ( lower ), the decameric c ring (Protein Data Bank ID code 2DB4 ( 53 ) r ainbow colors ) and a subunit model ( red ) have been fitted to the PA1b-streptavidin-HRP V-ATPase reconstruction in the absence of ATP using Chimera. If catalytically active, the c ring would rotate counterclockwise with respect to subunit a when observed from this perspective.

    Journal: The Journal of Biological Chemistry

    Article Title: PA1b Inhibitor Binding to Subunits c and e of the Vacuolar ATPase Reveals Its Insecticidal Mechanism *

    doi: 10.1074/jbc.M113.541250

    Figure Lengend Snippet: Electron microscopy of PA1b-bound V-ATPase. A , representative classes of PA1b-streptavidin-HRP-bound V-ATPase in the absence of ATP. B , as A but in the presence of 2 m m Mg·ATP. The PA1b-streptavidin-HRP density is indicated by an arrow in the far left panel 1 of A. Scale bars in both A and B represent 15 nm. C–E , three-dimensional reconstructions of the V-ATPase viewed perpendicular to the long axis of the complex ( upper image ) and from the extracellular end ( lower image ) bound to PA1b ( C ), bound to PA1b after the addition of Mg·ATP ( D ) and a control with no PA1b ( E ). All models were generated using EMAN, and the picture was produced using Chimera rendered at the same sigma level. In C ( lower ), the decameric c ring (Protein Data Bank ID code 2DB4 ( 53 ) r ainbow colors ) and a subunit model ( red ) have been fitted to the PA1b-streptavidin-HRP V-ATPase reconstruction in the absence of ATP using Chimera. If catalytically active, the c ring would rotate counterclockwise with respect to subunit a when observed from this perspective.

    Article Snippet: For the second experiment, 4 μl of V-ATPase (4 μg) was mixed with 3 μl of biotin-PA1b (3 μg) and 3 μl of streptavidin-HRP (15 μg), made up to 60 μl using V-ATPase buffer and incubated for 30 min. Mg·ATP was from a stock solution of 100 mm at pH 7.5 to a final concentration of 5 mm , and the mixture was incubated at room temperature for 5 min to allow for complete turnover.

    Techniques: Electron Microscopy, Generated, Produced

    Expression of recombinant porcine CCL2. (A) CHO cell line stably expressing the porcine CCL2 fused to GFP. The expression of GFP fusion protein was directly analysed by flow cytometry. Non transfected CHO cells were used as negative control (grey histogram). 5 000 cells were acquired. (B) Western blot of CCL2-GFP produced by transfected CHO cells. Different dilutions of supernatant were resolved by 15% SDS-PAGE under reducing conditions and revealed with biotinylated anti-GFP and streptavidin-HRP. Numbers on the left indicate the position of MW markers. (C) Chemotactic activity of CCL2-GFP on porcine blood monocytes. Chemotaxis was assessed with the Transwell cell migration system and subsequent flow cytometry counting of migrated cells by a 45 s acquisition. (1) FSC versus SSC dot plot of migrated cells in response to supernatants from CHO cells expressing CCL2-GFP or the inverted sequence of pCCL2 fused to GFP (InvCCL2-GFP, negative control). (2) Results expressed as migration index, calculated as the ratio of the number of cells migrating to the chemokine and the number of cells in the negative control. Results from one representative experiment out of three performed are shown. (A color version of this figure is available at www.vetres.org. )

    Journal: Veterinary Research

    Article Title: Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2

    doi: 10.1051/vetres/2010048

    Figure Lengend Snippet: Expression of recombinant porcine CCL2. (A) CHO cell line stably expressing the porcine CCL2 fused to GFP. The expression of GFP fusion protein was directly analysed by flow cytometry. Non transfected CHO cells were used as negative control (grey histogram). 5 000 cells were acquired. (B) Western blot of CCL2-GFP produced by transfected CHO cells. Different dilutions of supernatant were resolved by 15% SDS-PAGE under reducing conditions and revealed with biotinylated anti-GFP and streptavidin-HRP. Numbers on the left indicate the position of MW markers. (C) Chemotactic activity of CCL2-GFP on porcine blood monocytes. Chemotaxis was assessed with the Transwell cell migration system and subsequent flow cytometry counting of migrated cells by a 45 s acquisition. (1) FSC versus SSC dot plot of migrated cells in response to supernatants from CHO cells expressing CCL2-GFP or the inverted sequence of pCCL2 fused to GFP (InvCCL2-GFP, negative control). (2) Results expressed as migration index, calculated as the ratio of the number of cells migrating to the chemokine and the number of cells in the negative control. Results from one representative experiment out of three performed are shown. (A color version of this figure is available at www.vetres.org. )

    Article Snippet: The expression of GFP-fused proteins in these clones was confirmed by Western blot using a biotin-conjugated goat anti-GFP polyclonal antibody and streptavidin-HRP.

    Techniques: Expressing, Recombinant, Stable Transfection, Flow Cytometry, Cytometry, Transfection, Negative Control, Western Blot, Produced, SDS Page, Activity Assay, Chemotaxis Assay, Migration, Sequencing

    Photoaffinity labeling of various PrP species. Streptavidin-HRP-probed blots of samples photoaffinity labeled with PA-PBD peptide. (A) Samples containing PrP Int1 or PrP C were incubated with or without PA-PBD and exposed to UV light for varying time periods, as indicated. (B) Samples containing α -helical PrP or PrP Int1 were incubated with PA-PBD and exposed to UV light for 5 min. (C) Samples of PrP Int1 were incubated with varying concentrations of PA-PBD, as indicated, and exposed to UV light for 0 or 5 min, as indicated. (D) Sample containing 7 μ g of PrP Int1 photoaffinity labeled with PA-PBD (PA-PrP Int1 ) is compared to a standard curve of biotinylated AviTag PrP for reference.

    Journal: Biochemistry

    Article Title: Prion Nucleation Site Unmasked by Transient Interaction with Phospholipid Cofactor

    doi: 10.1021/bi4014825

    Figure Lengend Snippet: Photoaffinity labeling of various PrP species. Streptavidin-HRP-probed blots of samples photoaffinity labeled with PA-PBD peptide. (A) Samples containing PrP Int1 or PrP C were incubated with or without PA-PBD and exposed to UV light for varying time periods, as indicated. (B) Samples containing α -helical PrP or PrP Int1 were incubated with PA-PBD and exposed to UV light for 5 min. (C) Samples of PrP Int1 were incubated with varying concentrations of PA-PBD, as indicated, and exposed to UV light for 0 or 5 min, as indicated. (D) Sample containing 7 μ g of PrP Int1 photoaffinity labeled with PA-PBD (PA-PrP Int1 ) is compared to a standard curve of biotinylated AviTag PrP for reference.

    Article Snippet: The resulting photoaffinity-labeled molecules were run on SDS-PAGE, transferred to PVDF, blocked with a 2.5% solution of bovine serum albumin (Fisher Scientific, Pittsburgh, PA), and incubated with streptavidin-conjugated HRP (ThermoFisher Scientific, Rockford, IL) at a 1:10 000 dilution before being washed with TBST and developed with SuperSignal West Femto maximum sensitivity substrate (ThermoFisher Scientific, Rockford, IL).

    Techniques: Labeling, Incubation