horseradish peroxidase hrp labelled goat antimouse igg secondary antibody  (Abcam)

 
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    Name:
    Goat F ab 2 Anti Mouse IgG Fc HRP
    Description:

    Catalog Number:
    AB98741
    Price:
    None
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    Structured Review

    Abcam horseradish peroxidase hrp labelled goat antimouse igg secondary antibody
    Schematic representation of the arrays of nanoelectrodes (NEA)-gliadin. The polycarbonate (PC) surface is exploited to immobilize the antigen (Ag) protein, gliadin. The assay is performed by molecular recognition of the target by the primary antibody anti-gliadin <t>IgG</t> and a subsequent secondary antibody anti-IgG labeled with horse radish peroxidase <t>(HRP)</t> enzyme. The electrocatalytic cycle was generated by adding the enzyme substrate H 2 O 2 and the mediator methylene blue (MB).

    https://www.bioz.com/result/horseradish peroxidase hrp labelled goat antimouse igg secondary antibody/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp labelled goat antimouse igg secondary antibody - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "Nanoelectrode Arrays Fabricated by Thermal Nanoimprint Lithography for Biosensing Application"

    Article Title: Nanoelectrode Arrays Fabricated by Thermal Nanoimprint Lithography for Biosensing Application

    Journal: Biosensors

    doi: 10.3390/bios10080090

    Schematic representation of the arrays of nanoelectrodes (NEA)-gliadin. The polycarbonate (PC) surface is exploited to immobilize the antigen (Ag) protein, gliadin. The assay is performed by molecular recognition of the target by the primary antibody anti-gliadin IgG and a subsequent secondary antibody anti-IgG labeled with horse radish peroxidase (HRP) enzyme. The electrocatalytic cycle was generated by adding the enzyme substrate H 2 O 2 and the mediator methylene blue (MB).
    Figure Legend Snippet: Schematic representation of the arrays of nanoelectrodes (NEA)-gliadin. The polycarbonate (PC) surface is exploited to immobilize the antigen (Ag) protein, gliadin. The assay is performed by molecular recognition of the target by the primary antibody anti-gliadin IgG and a subsequent secondary antibody anti-IgG labeled with horse radish peroxidase (HRP) enzyme. The electrocatalytic cycle was generated by adding the enzyme substrate H 2 O 2 and the mediator methylene blue (MB).

    Techniques Used: Labeling, Generated

    Related Articles

    Blocking Assay:

    Article Title: Application of an O-Linked Glycosylation System in Yersinia enterocolitica Serotype O:9 to Generate a New Candidate Vaccine against Brucella abortus
    Article Snippet: .. The plates were patted dry and Blocking Buffer (PBS + 5% milk powder) was added to each well followed by incubation at 37 °C for 2 h. After drying the plates, diluted serum from the immunized mice was added to each well and the plate was incubated at 37 °C for 1 h. After another washing and drying step, 1:15,000 diluted HRP-conjugated goat anti-mouse IgG antibody (Abcam, Shanghai, China) was added to each well and incubated at 37 °C for 1 h. The washing and drying step was repeated. .. The Soluble TMB Kit (CWbio, Beijing, China) was used to initiate the detection reaction.

    Incubation:

    Article Title: Application of an O-Linked Glycosylation System in Yersinia enterocolitica Serotype O:9 to Generate a New Candidate Vaccine against Brucella abortus
    Article Snippet: .. The plates were patted dry and Blocking Buffer (PBS + 5% milk powder) was added to each well followed by incubation at 37 °C for 2 h. After drying the plates, diluted serum from the immunized mice was added to each well and the plate was incubated at 37 °C for 1 h. After another washing and drying step, 1:15,000 diluted HRP-conjugated goat anti-mouse IgG antibody (Abcam, Shanghai, China) was added to each well and incubated at 37 °C for 1 h. The washing and drying step was repeated. .. The Soluble TMB Kit (CWbio, Beijing, China) was used to initiate the detection reaction.

    Article Title: Active immunization with Tocilizumab mimotopes induces specific immune responses
    Article Snippet: ELISA plates were coated with rIL-6R (10 μg/ml) overnight at 4°C. .. After the plates were washed and blocked, pooled serum were added and incubated for 2 h. Then bound antibodies were then incubated with HRP–conjugated Fc-specific goat ant-mouse IgG (Abcam, Cambridge, UK). ..

    Article Title: Ror2, a Developmentally Regulated Kinase, Is Associated With Tumor Growth, Apoptosis, Migration, and Invasion in Renal Cell Carcinoma
    Article Snippet: RCC tissue sections were initially treated with deparaffinization and hydration, heated in EDTA (pH 8.0), and incubated with 3% hydrogen peroxide for 10 min for antigen retrieval. .. The reaction of Ror2 mouse polyclonal antibody (Abcam) took place for 1 h at room temperature, following incubation with goat anti-mouse horseradish peroxidase-conjugated IgG (Abcam). ..

    Mouse Assay:

    Article Title: Application of an O-Linked Glycosylation System in Yersinia enterocolitica Serotype O:9 to Generate a New Candidate Vaccine against Brucella abortus
    Article Snippet: .. The plates were patted dry and Blocking Buffer (PBS + 5% milk powder) was added to each well followed by incubation at 37 °C for 2 h. After drying the plates, diluted serum from the immunized mice was added to each well and the plate was incubated at 37 °C for 1 h. After another washing and drying step, 1:15,000 diluted HRP-conjugated goat anti-mouse IgG antibody (Abcam, Shanghai, China) was added to each well and incubated at 37 °C for 1 h. The washing and drying step was repeated. .. The Soluble TMB Kit (CWbio, Beijing, China) was used to initiate the detection reaction.

    Recombinant:

    Article Title: Nanoelectrode Arrays Fabricated by Thermal Nanoimprint Lithography for Biosensing Application
    Article Snippet: .. Recombinant Wheat Gliadin protein (19 kDa), mouse monoclonal antigliadin antibody, and horseradish peroxidase (HRP)-labelled goat antimouse IgG secondary antibody were purchased from Abcam® (Cambridge, UK). ..

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  • 94
    Abcam horseradish peroxidase hrp labelled goat antimouse igg secondary antibody
    Schematic representation of the arrays of nanoelectrodes (NEA)-gliadin. The polycarbonate (PC) surface is exploited to immobilize the antigen (Ag) protein, gliadin. The assay is performed by molecular recognition of the target by the primary antibody anti-gliadin <t>IgG</t> and a subsequent secondary antibody anti-IgG labeled with horse radish peroxidase <t>(HRP)</t> enzyme. The electrocatalytic cycle was generated by adding the enzyme substrate H 2 O 2 and the mediator methylene blue (MB).
    Horseradish Peroxidase Hrp Labelled Goat Antimouse Igg Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp labelled goat antimouse igg secondary antibody/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp labelled goat antimouse igg secondary antibody - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    86
    Abcam goat antimouse igg horseradish peroxidase hrp labeled secondary antibody
    Schematic representation of the arrays of nanoelectrodes (NEA)-gliadin. The polycarbonate (PC) surface is exploited to immobilize the antigen (Ag) protein, gliadin. The assay is performed by molecular recognition of the target by the primary antibody anti-gliadin <t>IgG</t> and a subsequent secondary antibody anti-IgG labeled with horse radish peroxidase <t>(HRP)</t> enzyme. The electrocatalytic cycle was generated by adding the enzyme substrate H 2 O 2 and the mediator methylene blue (MB).
    Goat Antimouse Igg Horseradish Peroxidase Hrp Labeled Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat antimouse igg horseradish peroxidase hrp labeled secondary antibody/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat antimouse igg horseradish peroxidase hrp labeled secondary antibody - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    97
    Abcam goat anti mouse igg h l hrp
    Schematic representation of the arrays of nanoelectrodes (NEA)-gliadin. The polycarbonate (PC) surface is exploited to immobilize the antigen (Ag) protein, gliadin. The assay is performed by molecular recognition of the target by the primary antibody anti-gliadin <t>IgG</t> and a subsequent secondary antibody anti-IgG labeled with horse radish peroxidase <t>(HRP)</t> enzyme. The electrocatalytic cycle was generated by adding the enzyme substrate H 2 O 2 and the mediator methylene blue (MB).
    Goat Anti Mouse Igg H L Hrp, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg h l hrp/product/Abcam
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg h l hrp - by Bioz Stars, 2021-06
    97/100 stars
      Buy from Supplier

    Image Search Results


    Schematic representation of the arrays of nanoelectrodes (NEA)-gliadin. The polycarbonate (PC) surface is exploited to immobilize the antigen (Ag) protein, gliadin. The assay is performed by molecular recognition of the target by the primary antibody anti-gliadin IgG and a subsequent secondary antibody anti-IgG labeled with horse radish peroxidase (HRP) enzyme. The electrocatalytic cycle was generated by adding the enzyme substrate H 2 O 2 and the mediator methylene blue (MB).

    Journal: Biosensors

    Article Title: Nanoelectrode Arrays Fabricated by Thermal Nanoimprint Lithography for Biosensing Application

    doi: 10.3390/bios10080090

    Figure Lengend Snippet: Schematic representation of the arrays of nanoelectrodes (NEA)-gliadin. The polycarbonate (PC) surface is exploited to immobilize the antigen (Ag) protein, gliadin. The assay is performed by molecular recognition of the target by the primary antibody anti-gliadin IgG and a subsequent secondary antibody anti-IgG labeled with horse radish peroxidase (HRP) enzyme. The electrocatalytic cycle was generated by adding the enzyme substrate H 2 O 2 and the mediator methylene blue (MB).

    Article Snippet: Recombinant Wheat Gliadin protein (19 kDa), mouse monoclonal antigliadin antibody, and horseradish peroxidase (HRP)-labelled goat antimouse IgG secondary antibody were purchased from Abcam® (Cambridge, UK).

    Techniques: Labeling, Generated