Horseradish Peroxidase Hrp Conjugated Rabbit Antimouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Proteomic surveillance of retinal autoantigens in endogenous uveitis: implication of esterase D and brain-type creatine kinase as novel autoantigens"
Article Title: Proteomic surveillance of retinal autoantigens in endogenous uveitis: implication of esterase D and brain-type creatine kinase as novel autoantigens
Journal: Molecular Vision
Figure Legend Snippet: The presence of antibodies to bAct, EsteD, and BB-CK in EAU mice was confirmed by 1D-WB and ELISA. A-C : Protein (2 μg) was subjected to 12.5% SDS–PAGE, transferred onto nitrocellulose membranes, then incubated with 1:200 sera from experimental autoimmune uveoretinitis (EAU) or control mice and 1:2000 horse radish peroxide (HRP) antimouse IgG as described in Methods. EAU serum samples were 8/10 positive in bAct ( A ), 6/10 positive in EsteD ( B ), and 4/10 positive in brain-type creatine kinase (BB-CK; C ). D-F : Antibody titer of the sera from the EAU or control mice was determined by ELISA. The antibody titer was calculated as binding units according to the formula shown in Methods. EAU serum samples were 4/10 positive in bAct ( D ), 7/10 positive in EsteD ( E ), and 4/10 positive in BB-CK ( F ). Lane 1 is ponseau S staining, lane 2-4 are membranes incubated with EAU sera, and lane 5-7 are membranes incubated with control sera. In the figure, M represents molecular weight marker.
Techniques Used: Mouse Assay, Western Blot, Enzyme-linked Immunosorbent Assay, SDS Page, Incubation, Binding Assay, Staining, Molecular Weight, Marker
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Article Snippet: The secondary antibodies were
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Article Snippet: Programmed cell death (PCD)
Article Title: The Polybasic Juxtamembrane Region of Sso1p Is Required for SNARE Function In Vivo †
Article Snippet: Secondary antibodies included goat antimouse immunoglobulin G (IgG) horseradish peroxidase from Rockland Immunochemicals, goat antirabbit IgG Fc horseradish peroxidase from Pierce, and
Article Title: Phosphorylated mTOR expression correlates with podoplanin expression and high tumor grade in esophageal squamous cell carcinoma
Article Snippet: .. Sections were incubated with primary antibody at 4°C overnight, followed with
Article Title: Agonist-Dependent Downregulation of Progesterone Receptors in Human Cervical Stromal Fibroblasts
Article Snippet: Proteins were then transferred to nitrocellulose membranes using either traditional wet transfer methods or the iBlot dry blotting system (Life Technologies, Grand Island, NY). .. After blocking with 5% nonfat dry milk/Tris-buffered saline and 0.2% Tween-20 (pH 7.8), membranes were probed with mouse anti-human PR (1:1000, Dako, Capinteria, CA) by
Article Title: Recombinant Immunoglobulin A Specific for Influenza A Virus Hemagglutinin: Production, Functional Analysis, and Formation of Secretory Immunoglobulin A
Article Snippet: 2-[(4-Hydroxyethyl)-1-piperazine]-ethanesulfonic acid (HEPES), Tween-20, kanamycin sulfate, hygromycine B, methanol, 4-chloro-1-naphthol, ortho-phenylenediamine, and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) were purchased from Wako Pure Chemicals; Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (F12) from Nissui Pharmaceuticals; Hybridoma-SFM, Eagle's Minimum Essential Medium, zeocin, and MagicMark™ XP Western Protein standards from Life Technologies; G418 from Nacalai Tesque; HyClone fetal bovine serum (FBS) from GE Healthcare; and a DNA size marker kit (100-bp DNA Ladder) from Takara. .. Horseradish peroxidase (HRP)-rabbit antimouse IgG, goat antimouse IgG, rabbit antimouse IgA (α chain-specific), and HRP-goat antirabbit IgG were purchased from