horseradish peroxidase hrp conjugated rabbit antimouse igg  (Thermo Fisher)


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    Structured Review

    Thermo Fisher horseradish peroxidase hrp conjugated rabbit antimouse igg
    The presence of antibodies to bAct, EsteD, and BB-CK in EAU mice was confirmed by 1D-WB and ELISA. A-C : Protein (2 μg) was subjected to 12.5% SDS–PAGE, transferred onto nitrocellulose membranes, then incubated with 1:200 sera from experimental autoimmune uveoretinitis (EAU) or control mice and 1:2000 horse radish peroxide <t>(HRP)</t> <t>antimouse</t> <t>IgG</t> as described in Methods. EAU serum samples were 8/10 positive in bAct ( A ), 6/10 positive in EsteD ( B ), and 4/10 positive in brain-type creatine kinase (BB-CK; C ). D-F : Antibody titer of the sera from the EAU or control mice was determined by ELISA. The antibody titer was calculated as binding units according to the formula shown in Methods. EAU serum samples were 4/10 positive in bAct ( D ), 7/10 positive in EsteD ( E ), and 4/10 positive in BB-CK ( F ). Lane 1 is ponseau S staining, lane 2-4 are membranes incubated with EAU sera, and lane 5-7 are membranes incubated with control sera. In the figure, M represents molecular weight marker.
    Horseradish Peroxidase Hrp Conjugated Rabbit Antimouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated rabbit antimouse igg/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated rabbit antimouse igg - by Bioz Stars, 2021-04
    99/100 stars

    Images

    1) Product Images from "Proteomic surveillance of retinal autoantigens in endogenous uveitis: implication of esterase D and brain-type creatine kinase as novel autoantigens"

    Article Title: Proteomic surveillance of retinal autoantigens in endogenous uveitis: implication of esterase D and brain-type creatine kinase as novel autoantigens

    Journal: Molecular Vision

    doi:

    The presence of antibodies to bAct, EsteD, and BB-CK in EAU mice was confirmed by 1D-WB and ELISA. A-C : Protein (2 μg) was subjected to 12.5% SDS–PAGE, transferred onto nitrocellulose membranes, then incubated with 1:200 sera from experimental autoimmune uveoretinitis (EAU) or control mice and 1:2000 horse radish peroxide (HRP) antimouse IgG as described in Methods. EAU serum samples were 8/10 positive in bAct ( A ), 6/10 positive in EsteD ( B ), and 4/10 positive in brain-type creatine kinase (BB-CK; C ). D-F : Antibody titer of the sera from the EAU or control mice was determined by ELISA. The antibody titer was calculated as binding units according to the formula shown in Methods. EAU serum samples were 4/10 positive in bAct ( D ), 7/10 positive in EsteD ( E ), and 4/10 positive in BB-CK ( F ). Lane 1 is ponseau S staining, lane 2-4 are membranes incubated with EAU sera, and lane 5-7 are membranes incubated with control sera. In the figure, M represents molecular weight marker.
    Figure Legend Snippet: The presence of antibodies to bAct, EsteD, and BB-CK in EAU mice was confirmed by 1D-WB and ELISA. A-C : Protein (2 μg) was subjected to 12.5% SDS–PAGE, transferred onto nitrocellulose membranes, then incubated with 1:200 sera from experimental autoimmune uveoretinitis (EAU) or control mice and 1:2000 horse radish peroxide (HRP) antimouse IgG as described in Methods. EAU serum samples were 8/10 positive in bAct ( A ), 6/10 positive in EsteD ( B ), and 4/10 positive in brain-type creatine kinase (BB-CK; C ). D-F : Antibody titer of the sera from the EAU or control mice was determined by ELISA. The antibody titer was calculated as binding units according to the formula shown in Methods. EAU serum samples were 4/10 positive in bAct ( D ), 7/10 positive in EsteD ( E ), and 4/10 positive in BB-CK ( F ). Lane 1 is ponseau S staining, lane 2-4 are membranes incubated with EAU sera, and lane 5-7 are membranes incubated with control sera. In the figure, M represents molecular weight marker.

    Techniques Used: Mouse Assay, Western Blot, Enzyme-linked Immunosorbent Assay, SDS Page, Incubation, Binding Assay, Staining, Molecular Weight, Marker

    Related Articles

    other:

    Article Title: BMI1 is directly regulated by androgen receptor to promote castration-resistance in prostate cancer
    Article Snippet: The secondary antibodies were Clean-Blot IP Detection Reagent (HRP, 21230, Thermo Scientific), goat antimouse IgG (H+L)-HRP (SA001–500, GenDEPOT), or goat anti-rabbit IgG (H+L)-HRP (SA002-500, GenDEPOT).

    Article Title: Tudor staphylococcal nuclease is an evolutionarily conserved component of the programmed cell death degradome.
    Article Snippet: Programmed cell death (PCD) is executed by proteases, which cleave diverse proteins thus modulating their biochemical and cellular functions.

    Article Title: The Polybasic Juxtamembrane Region of Sso1p Is Required for SNARE Function In Vivo †
    Article Snippet: Secondary antibodies included goat antimouse immunoglobulin G (IgG) horseradish peroxidase from Rockland Immunochemicals, goat antirabbit IgG Fc horseradish peroxidase from Pierce, and Alexafluor 488 goat antimouse IgG from Molecular Probes.

    Incubation:

    Article Title: Phosphorylated mTOR expression correlates with podoplanin expression and high tumor grade in esophageal squamous cell carcinoma
    Article Snippet: .. Sections were incubated with primary antibody at 4°C overnight, followed with poly-horseradish peroxidase (HRP) antimouse/rabbit IgG reagent (Zymed) to localize the primary antibody, and diaminobenzidine (DAB) was used to visualize the complex. ..

    Blocking Assay:

    Article Title: Agonist-Dependent Downregulation of Progesterone Receptors in Human Cervical Stromal Fibroblasts
    Article Snippet: Proteins were then transferred to nitrocellulose membranes using either traditional wet transfer methods or the iBlot dry blotting system (Life Technologies, Grand Island, NY). .. After blocking with 5% nonfat dry milk/Tris-buffered saline and 0.2% Tween-20 (pH 7.8), membranes were probed with mouse anti-human PR (1:1000, Dako, Capinteria, CA) by rabbit antimouse IgG-horseradish peroxidase (1:2000, Thermo Scientific, Pittsburgh, PA). .. Antibody binding was detected using SuperSignal chemiluminescent substrate (Thermo Scientific, Pittsburgh, PA) in concert with a VersaDoc Imaging System (Bio-Rad, Hercules, CA).

    Recombinant:

    Article Title: Recombinant Immunoglobulin A Specific for Influenza A Virus Hemagglutinin: Production, Functional Analysis, and Formation of Secretory Immunoglobulin A
    Article Snippet: 2-[(4-Hydroxyethyl)-1-piperazine]-ethanesulfonic acid (HEPES), Tween-20, kanamycin sulfate, hygromycine B, methanol, 4-chloro-1-naphthol, ortho-phenylenediamine, and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) were purchased from Wako Pure Chemicals; Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (F12) from Nissui Pharmaceuticals; Hybridoma-SFM, Eagle's Minimum Essential Medium, zeocin, and MagicMark™ XP Western Protein standards from Life Technologies; G418 from Nacalai Tesque; HyClone fetal bovine serum (FBS) from GE Healthcare; and a DNA size marker kit (100-bp DNA Ladder) from Takara. .. Horseradish peroxidase (HRP)-rabbit antimouse IgG, goat antimouse IgG, rabbit antimouse IgA (α chain-specific), and HRP-goat antirabbit IgG were purchased from Zymed; HRP-goat antimouse IgA (α chain-specific), HRP-goat antimouse kappa, goat antimouse kappa, and HRP-donkey antigoat IgG (H+L) from Southern Biotech; TEPC 15, MOPC 21, and bovine serum albumin (BSA) from Sigma; rabbit antimouse J chain and HRP-donkey antigoat IgG from Santa Cruz Biotechnology; goat antimouse polymeric Ig receptor (pIgR) and recombinant mouse pIgR from R & D Systems; rabbit antimouse pIgR from Sino Biological, Inc.; and HRP-goat antimouse IgG+M from Jackson Immuno Research. .. Can Get Signal Solution 1, Can Get Signal Solution 2, and PVDF Blocking Reagent were purchased from TOYOBO; and N,N -diethyl-para-phenylendiamine from Kanto Chemicals.

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    Thermo Fisher hrp conjugated secondary antibodies
    Hrp Conjugated Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated secondary antibodies/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated secondary antibodies - by Bioz Stars, 2021-04
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    99
    Thermo Fisher horseradish peroxidase hrp conjugated rabbit antimouse igg
    The presence of antibodies to bAct, EsteD, and BB-CK in EAU mice was confirmed by 1D-WB and ELISA. A-C : Protein (2 μg) was subjected to 12.5% SDS–PAGE, transferred onto nitrocellulose membranes, then incubated with 1:200 sera from experimental autoimmune uveoretinitis (EAU) or control mice and 1:2000 horse radish peroxide <t>(HRP)</t> <t>antimouse</t> <t>IgG</t> as described in Methods. EAU serum samples were 8/10 positive in bAct ( A ), 6/10 positive in EsteD ( B ), and 4/10 positive in brain-type creatine kinase (BB-CK; C ). D-F : Antibody titer of the sera from the EAU or control mice was determined by ELISA. The antibody titer was calculated as binding units according to the formula shown in Methods. EAU serum samples were 4/10 positive in bAct ( D ), 7/10 positive in EsteD ( E ), and 4/10 positive in BB-CK ( F ). Lane 1 is ponseau S staining, lane 2-4 are membranes incubated with EAU sera, and lane 5-7 are membranes incubated with control sera. In the figure, M represents molecular weight marker.
    Horseradish Peroxidase Hrp Conjugated Rabbit Antimouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated rabbit antimouse igg/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated rabbit antimouse igg - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher peroxidase hrp conjugated rabbit antimouse igg1 antibody
    The presence of antibodies to bAct, EsteD, and BB-CK in EAU mice was confirmed by 1D-WB and ELISA. A-C : Protein (2 μg) was subjected to 12.5% SDS–PAGE, transferred onto nitrocellulose membranes, then incubated with 1:200 sera from experimental autoimmune uveoretinitis (EAU) or control mice and 1:2000 horse radish peroxide <t>(HRP)</t> <t>antimouse</t> <t>IgG</t> as described in Methods. EAU serum samples were 8/10 positive in bAct ( A ), 6/10 positive in EsteD ( B ), and 4/10 positive in brain-type creatine kinase (BB-CK; C ). D-F : Antibody titer of the sera from the EAU or control mice was determined by ELISA. The antibody titer was calculated as binding units according to the formula shown in Methods. EAU serum samples were 4/10 positive in bAct ( D ), 7/10 positive in EsteD ( E ), and 4/10 positive in BB-CK ( F ). Lane 1 is ponseau S staining, lane 2-4 are membranes incubated with EAU sera, and lane 5-7 are membranes incubated with control sera. In the figure, M represents molecular weight marker.
    Peroxidase Hrp Conjugated Rabbit Antimouse Igg1 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase hrp conjugated rabbit antimouse igg1 antibody/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase hrp conjugated rabbit antimouse igg1 antibody - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    The presence of antibodies to bAct, EsteD, and BB-CK in EAU mice was confirmed by 1D-WB and ELISA. A-C : Protein (2 μg) was subjected to 12.5% SDS–PAGE, transferred onto nitrocellulose membranes, then incubated with 1:200 sera from experimental autoimmune uveoretinitis (EAU) or control mice and 1:2000 horse radish peroxide (HRP) antimouse IgG as described in Methods. EAU serum samples were 8/10 positive in bAct ( A ), 6/10 positive in EsteD ( B ), and 4/10 positive in brain-type creatine kinase (BB-CK; C ). D-F : Antibody titer of the sera from the EAU or control mice was determined by ELISA. The antibody titer was calculated as binding units according to the formula shown in Methods. EAU serum samples were 4/10 positive in bAct ( D ), 7/10 positive in EsteD ( E ), and 4/10 positive in BB-CK ( F ). Lane 1 is ponseau S staining, lane 2-4 are membranes incubated with EAU sera, and lane 5-7 are membranes incubated with control sera. In the figure, M represents molecular weight marker.

    Journal: Molecular Vision

    Article Title: Proteomic surveillance of retinal autoantigens in endogenous uveitis: implication of esterase D and brain-type creatine kinase as novel autoantigens

    doi:

    Figure Lengend Snippet: The presence of antibodies to bAct, EsteD, and BB-CK in EAU mice was confirmed by 1D-WB and ELISA. A-C : Protein (2 μg) was subjected to 12.5% SDS–PAGE, transferred onto nitrocellulose membranes, then incubated with 1:200 sera from experimental autoimmune uveoretinitis (EAU) or control mice and 1:2000 horse radish peroxide (HRP) antimouse IgG as described in Methods. EAU serum samples were 8/10 positive in bAct ( A ), 6/10 positive in EsteD ( B ), and 4/10 positive in brain-type creatine kinase (BB-CK; C ). D-F : Antibody titer of the sera from the EAU or control mice was determined by ELISA. The antibody titer was calculated as binding units according to the formula shown in Methods. EAU serum samples were 4/10 positive in bAct ( D ), 7/10 positive in EsteD ( E ), and 4/10 positive in BB-CK ( F ). Lane 1 is ponseau S staining, lane 2-4 are membranes incubated with EAU sera, and lane 5-7 are membranes incubated with control sera. In the figure, M represents molecular weight marker.

    Article Snippet: Horseradish peroxidase (HRP)-conjugated rabbit antimouse IgG and goat antihuman IgG were obtained from Zymed Laboratories (San Francisco, CA).

    Techniques: Mouse Assay, Western Blot, Enzyme-linked Immunosorbent Assay, SDS Page, Incubation, Binding Assay, Staining, Molecular Weight, Marker