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Santa Cruz Biotechnology horseradish peroxidase conjugated secondary antibody
Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 434 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibody/product/Santa Cruz Biotechnology
Average 92 stars, based on 434 article reviews
Price from $9.99 to $1999.99
horseradish peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
92/100 stars

Images

1) Product Images from "Proteomic analysis of primary duck hepatocytes infected with duck hepatitis B virus"

Article Title: Proteomic analysis of primary duck hepatocytes infected with duck hepatitis B virus

Journal: Proteome Science

doi: 10.1186/1477-5956-8-28

Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by horseradish peroxidase-conjugated goat anti-mouse IgG as secondary antibody (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
Figure Legend Snippet: Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by horseradish peroxidase-conjugated goat anti-mouse IgG as secondary antibody (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).

Techniques Used: Western Blot, Infection, SDS Page, Staining

2) Product Images from "Expression of Rho-kinase and its functional role in the contractile activity of the mouse vas deferens"

Article Title: Expression of Rho-kinase and its functional role in the contractile activity of the mouse vas deferens

Journal: British Journal of Pharmacology

doi: 10.1038/sj.bjp.0705479

Western blotting for Rho-kinase (ROCK-2, ROK α ) in the mouse vas deferens and rat mesenteric artery. Homogenates of the tissues were submitted to SDS – PAGE with 8% polyacrylamide and then transferred onto a PVDF. The membrane was blocked with an ECL advance blocking agent in Tris-buffered solution containing 0.05% TBS-T for 1 h. It was then probed with a primary antibody raised against ROCK-2 (polyclonal IgG) at 1 : 250 dilution followed by horseradish peroxidase-conjugated secondary antibody (donkey anti-goat, 1 : 500). Proteins bound with the antibodies were then visualized by the ECL Advance kit.
Figure Legend Snippet: Western blotting for Rho-kinase (ROCK-2, ROK α ) in the mouse vas deferens and rat mesenteric artery. Homogenates of the tissues were submitted to SDS – PAGE with 8% polyacrylamide and then transferred onto a PVDF. The membrane was blocked with an ECL advance blocking agent in Tris-buffered solution containing 0.05% TBS-T for 1 h. It was then probed with a primary antibody raised against ROCK-2 (polyclonal IgG) at 1 : 250 dilution followed by horseradish peroxidase-conjugated secondary antibody (donkey anti-goat, 1 : 500). Proteins bound with the antibodies were then visualized by the ECL Advance kit.

Techniques Used: Western Blot, SDS Page, Blocking Assay

3) Product Images from "Expression of Rho-kinase (ROCK-1 and ROCK-2) and its substantial role in the contractile activity of the sheep ureter"

Article Title: Expression of Rho-kinase (ROCK-1 and ROCK-2) and its substantial role in the contractile activity of the sheep ureter

Journal: British Journal of Pharmacology

doi: 10.1038/sj.bjp.0705961

Demonstration of the expressions of ROCK-1 and ROCK-2 in the sheep ureteric ( n =4) and aortic ( n =4) smooth muscle homogenates by Western blotting. The homogenates were submitted to SDS–PAGE with 8% polyacrylamide and then transferred to a nitrocellulose membrane (PVDF). The membrane was blocked with an ECL Advance blocking agent in Tris-buffered solution containing 0.05% Tween-20 (TBS-T) for 1 h. It was then probed with a primary antibody raised against ROCK-1 and ROCK-2 (Polyclonal IgG) at 1 : 200 dilution, followed by horseradish peroxidase-conjugated secondary antibody (donkey antigoat, 1 : 1000). Proteins bound with the antibodies were then visualized by the ECL Advance kit. Densitometric analysis of the protein bands was evaluated by a computer program (Scion image, U.S.A.).
Figure Legend Snippet: Demonstration of the expressions of ROCK-1 and ROCK-2 in the sheep ureteric ( n =4) and aortic ( n =4) smooth muscle homogenates by Western blotting. The homogenates were submitted to SDS–PAGE with 8% polyacrylamide and then transferred to a nitrocellulose membrane (PVDF). The membrane was blocked with an ECL Advance blocking agent in Tris-buffered solution containing 0.05% Tween-20 (TBS-T) for 1 h. It was then probed with a primary antibody raised against ROCK-1 and ROCK-2 (Polyclonal IgG) at 1 : 200 dilution, followed by horseradish peroxidase-conjugated secondary antibody (donkey antigoat, 1 : 1000). Proteins bound with the antibodies were then visualized by the ECL Advance kit. Densitometric analysis of the protein bands was evaluated by a computer program (Scion image, U.S.A.).

Techniques Used: Western Blot, SDS Page, Blocking Assay

Related Articles

Incubation:

Article Title: Role of Transforming Growth Factor-β1 and Smads Signaling Pathway in Intrauterine Adhesion
Article Snippet: .. The membranes were then incubated with primary antibodies against Smad3, P-Smad3 (1 : 200; Santa Cruz, CA, USA), Smad7 (1 : 500; Santa Cruz, CA, USA), TGFβ 1 (1 : 200; Santa Cruz, CA, USA), or β -actin (1 : 4000; Santa Cruz, CA, USA) with gentle agitation at 4°C for 18 h, followed by horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG, 1 : 1000; Santa Cruz; anti-mouse IgG, 1 : 6000; Santa Cruz) for 60 min at room temperature. .. The protein bands were determined by Luminata Crescendo Western HRP Substrate (Millipore, Billerica, MA, USA) through Molecular Imager ChemiDoc XRS System (Bio-Rad, Philadelphia, PA, USA).

Article Title: Proteomic analysis of primary duck hepatocytes infected with duck hepatitis B virus
Article Snippet: .. The blots were then washed four times with PBS containing 0.1% Tween-20, and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology) 1 hour at room temperature. .. After washed four times with PBS containing 0.1% Tween-20, the bands were developed with ECL detection reagent (Pierce).

other:

Article Title: Norcantharidin Induces Human Melanoma A375-S2 Cell Apoptosis through Mitochondrial and Caspase Pathways
Article Snippet: Caspase-9 inhibitor (Ac-LEHD-CHO), rabbit polyclonal antibodies against ICAD, cytochrome c , Bax and Bcl-xL , mouse polyclonal antibodies against Bcl-2, horseradish peroxidase-conjugated secondary antibody (goat-anti-rabbit or goat-anti-mouse) were from Santa Cruz Biotechnology (Santa Cruz, CA).

Blocking Assay:

Article Title: Expression of Rho-kinase and its functional role in the contractile activity of the mouse vas deferens
Article Snippet: .. The membrane was blocked with the blocking agent of ECL Advance kit (Amersham Bio-sciences, Freiburg, Germany) in Tris-buffered solution containing 0.05% Tween-20 (TBS-T) for 1 h. It was then probed with a primary antibody raised against ROCK-2 (ROK α , Polyclonal IgG, sc-1851, Santa Cruz Biotechnology Inc., CA, U.S.A.) at 1 : 250 dilution followed by horseradish peroxidase-conjugated secondary antibody (donkey anti-goat, 1 : 500, Santa Cruz Biotechnology Inc., CA, U.S.A.). .. The blots were then detected with the ECL Advance kit (Amersham Biosciences, Freiburg, Germany).

Article Title: Expression of Rho-kinase (ROCK-1 and ROCK-2) and its substantial role in the contractile activity of the sheep ureter
Article Snippet: .. The membrane was blocked with the blocking agent of enhanced chemiluminescence (ECL advance) kit (Amersham Biosciences, Freiburg, Germany) in Tris-buffered solution containing 0.05% Tween-20 (TBS-T) for 1 h. It was then probed with primary antibodies raised against ROCK-1 (ROK β ) or ROCK-2 (ROK α , Polyclonal IgG, Santa Cruz Biotechnology Inc., CA, U.S.A.) at 1 : 200 dilution, followed by horseradish peroxidase-conjugated secondary antibody (donkey antigoat, 1 : 1000, Santa Cruz Biotechnology Inc., CA, U.S.A.). .. The blots were then detected with the advanced chemiluminescence detection kit (Amersham Biosciences, Freiburg, Germany).

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  • 88
    Santa Cruz Biotechnology hrp conjugated goat antirabbit igg
    SOCS3 expression in peripheral blood eosinophils from Th2 patients analyzed by immunohistochemical, immunofluorescence, and Western blot techniques. Eosinophils from asthmatic and NAEB patients within healthy controls were adhered to slides and incubated with peroxidase-conjugated goat <t>antirabbit</t> <t>IgG</t> against SOCS3 antibody ((b), (c), and (d)) or rabbit IgG as a control (a), and Texas Red conjugated goat antirabbit IgG against SOCS3 antibody ((f), (g), and (h)) or rabbit IgG as a control (e). The slides were observed by optical ((a), (b), (c), (d)) or confocal ((e), (f), (g) and (h)) microscopy. Western blot analysis of the cytosolic extract of purified eosinophils was achieved using antibody against SOCS3 (i). Lane 1: recombinant SOCS3 was loaded as a positive control; lane 2: eosinophil lysate from NAEB patients; lane 3: eosinophil lysate from healthy control patients; lane 4: isotype negative control. The picture is a representative example of 5 individuals, all displaying similar results. (j): SOCS3 bands were quantified by densitometry and corrected by actin expression; data are expressed as the mean ± SD, n = 5, * P
    Hrp Conjugated Goat Antirabbit Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat antirabbit igg/product/Santa Cruz Biotechnology
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat antirabbit igg - by Bioz Stars, 2020-07
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    92
    Santa Cruz Biotechnology horseradish peroxidase conjugated secondary antibody
    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by <t>horseradish</t> <t>peroxidase-conjugated</t> goat anti-mouse IgG as <t>secondary</t> <t>antibody</t> (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 434 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibody/product/Santa Cruz Biotechnology
    Average 92 stars, based on 434 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology hrp conjugated anti mouse igg antibody
    Expression of recombinant protein in ZYP-5052 medium. A) . Coomassie stained 12% SDS-PAGE of lysates shows proteins expressed in ZYP-5052 at 25°C at different incubation time. Equal culture densities (corresponding to 1 OD 600 cells) were analysed in each lane. Cells grown to saturation in PG, a non-inducing growth medium were loaded as control. Lane L: Protein marker; Lane C: Control; Lane 3 h-45 h: Induced samples at different incubation time, as indicated on top of each lane. B) . Western blot of expressed protein. Expressed protein was transferred to nitrocellulose membrane at 30 V overnight at 4°C. After blocking in 3% BSA, membrane was treated with mouse raised anti-His antibody (1 : 500) and <t>HRP-conjugated</t> anti-mouse <t>IgG</t> antibody and developed with DAB. Bold arrow denotes distinct band in induced sample corresponding to expressed protein of 25 kDa size. Control used is culture grown to saturation in PG medium. No expression is seen in control. Lane L: Protein marker; Lane C: Control; Lane 45 h: Sample processed 45 hr post incubation at 25°C.
    Hrp Conjugated Anti Mouse Igg Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated anti mouse igg antibody/product/Santa Cruz Biotechnology
    Average 92 stars, based on 6 article reviews
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    85
    Santa Cruz Biotechnology goat horseradish peroxidase hrp conjugated secondary anti mouse antibody
    <t>BtaE</t> localization. (A and C) Western blot analysis of whole-membrane fractions from B. suis (A) and E. coli pBBR btaE (C) was carried out. Membrane samples were submitted to a strong denaturing process. Samples were electrophoresed by SDS-PAGE and transferred to a PDVF membrane. Blots were incubated with anti-BtaE antisera and with anti-mouse <t>HRP-conjugated</t> secondary antibody and finally revealed using ECL Plus. On the right, a Coomassie blue pattern is shown as a loading control (ctrl). (B and D) Detection of BtaE (in red) on the B. suis surface (B) and on the E. coli pBBR btaE surface (D) by immunofluorescence of GFP-tagged bacteria. Cultures of GFP-labeled strains were fixed, incubated with anti-BtaE antibodies, and then probed with a CY3-conjugated donkey anti-mouse antibody preparation. Samples were observed with a Plan-Aprochromat 100×/1.4 oil DIC objective on a Zeiss LSM 5 Pascal confocal microscope.
    Goat Horseradish Peroxidase Hrp Conjugated Secondary Anti Mouse Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SOCS3 expression in peripheral blood eosinophils from Th2 patients analyzed by immunohistochemical, immunofluorescence, and Western blot techniques. Eosinophils from asthmatic and NAEB patients within healthy controls were adhered to slides and incubated with peroxidase-conjugated goat antirabbit IgG against SOCS3 antibody ((b), (c), and (d)) or rabbit IgG as a control (a), and Texas Red conjugated goat antirabbit IgG against SOCS3 antibody ((f), (g), and (h)) or rabbit IgG as a control (e). The slides were observed by optical ((a), (b), (c), (d)) or confocal ((e), (f), (g) and (h)) microscopy. Western blot analysis of the cytosolic extract of purified eosinophils was achieved using antibody against SOCS3 (i). Lane 1: recombinant SOCS3 was loaded as a positive control; lane 2: eosinophil lysate from NAEB patients; lane 3: eosinophil lysate from healthy control patients; lane 4: isotype negative control. The picture is a representative example of 5 individuals, all displaying similar results. (j): SOCS3 bands were quantified by densitometry and corrected by actin expression; data are expressed as the mean ± SD, n = 5, * P

    Journal: Clinical and Developmental Immunology

    Article Title: Suppressors of Cytokine Signaling 3 Expression in Eosinophils: Regulation by PGE2 and Th2 Cytokines

    doi: 10.1155/2011/917015

    Figure Lengend Snippet: SOCS3 expression in peripheral blood eosinophils from Th2 patients analyzed by immunohistochemical, immunofluorescence, and Western blot techniques. Eosinophils from asthmatic and NAEB patients within healthy controls were adhered to slides and incubated with peroxidase-conjugated goat antirabbit IgG against SOCS3 antibody ((b), (c), and (d)) or rabbit IgG as a control (a), and Texas Red conjugated goat antirabbit IgG against SOCS3 antibody ((f), (g), and (h)) or rabbit IgG as a control (e). The slides were observed by optical ((a), (b), (c), (d)) or confocal ((e), (f), (g) and (h)) microscopy. Western blot analysis of the cytosolic extract of purified eosinophils was achieved using antibody against SOCS3 (i). Lane 1: recombinant SOCS3 was loaded as a positive control; lane 2: eosinophil lysate from NAEB patients; lane 3: eosinophil lysate from healthy control patients; lane 4: isotype negative control. The picture is a representative example of 5 individuals, all displaying similar results. (j): SOCS3 bands were quantified by densitometry and corrected by actin expression; data are expressed as the mean ± SD, n = 5, * P

    Article Snippet: The secondary antibody, HRP-conjugated goat antirabbit IgG (Santa Cruz Biotechnology, Inc.), was diluted 1 : 1000.

    Techniques: Expressing, Immunohistochemistry, Immunofluorescence, Western Blot, Incubation, Microscopy, Purification, Recombinant, Positive Control, Negative Control

    Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by horseradish peroxidase-conjugated goat anti-mouse IgG as secondary antibody (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).

    Journal: Proteome Science

    Article Title: Proteomic analysis of primary duck hepatocytes infected with duck hepatitis B virus

    doi: 10.1186/1477-5956-8-28

    Figure Lengend Snippet: Confirmation of the differentially expressed proteins of annexin A2 and beta-actin by Western blot . The equal cell lysates of control and DHBV infected PDHs at 12, 24, 72 and 120 h post-infection were separated by SDS-PAGE, and detected by Western blotting with mouse anti-beta-actin and anti-duck annexin A2 as primary antibodies and then followed by horseradish peroxidase-conjugated goat anti-mouse IgG as secondary antibody (A). The same amount protein of each sample were applied to a parallel SDS-PAGE gel and stained with Coomassie brilliant blue (B).

    Article Snippet: The blots were then washed four times with PBS containing 0.1% Tween-20, and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology) 1 hour at room temperature.

    Techniques: Western Blot, Infection, SDS Page, Staining

    Expression of recombinant protein in ZYP-5052 medium. A) . Coomassie stained 12% SDS-PAGE of lysates shows proteins expressed in ZYP-5052 at 25°C at different incubation time. Equal culture densities (corresponding to 1 OD 600 cells) were analysed in each lane. Cells grown to saturation in PG, a non-inducing growth medium were loaded as control. Lane L: Protein marker; Lane C: Control; Lane 3 h-45 h: Induced samples at different incubation time, as indicated on top of each lane. B) . Western blot of expressed protein. Expressed protein was transferred to nitrocellulose membrane at 30 V overnight at 4°C. After blocking in 3% BSA, membrane was treated with mouse raised anti-His antibody (1 : 500) and HRP-conjugated anti-mouse IgG antibody and developed with DAB. Bold arrow denotes distinct band in induced sample corresponding to expressed protein of 25 kDa size. Control used is culture grown to saturation in PG medium. No expression is seen in control. Lane L: Protein marker; Lane C: Control; Lane 45 h: Sample processed 45 hr post incubation at 25°C.

    Journal: Virology Journal

    Article Title: Cloning and expression of N22 region of Torque Teno virus (TTV) genome and use of peptide in developing immunoassay for TTV antibodies

    doi: 10.1186/1743-422X-11-96

    Figure Lengend Snippet: Expression of recombinant protein in ZYP-5052 medium. A) . Coomassie stained 12% SDS-PAGE of lysates shows proteins expressed in ZYP-5052 at 25°C at different incubation time. Equal culture densities (corresponding to 1 OD 600 cells) were analysed in each lane. Cells grown to saturation in PG, a non-inducing growth medium were loaded as control. Lane L: Protein marker; Lane C: Control; Lane 3 h-45 h: Induced samples at different incubation time, as indicated on top of each lane. B) . Western blot of expressed protein. Expressed protein was transferred to nitrocellulose membrane at 30 V overnight at 4°C. After blocking in 3% BSA, membrane was treated with mouse raised anti-His antibody (1 : 500) and HRP-conjugated anti-mouse IgG antibody and developed with DAB. Bold arrow denotes distinct band in induced sample corresponding to expressed protein of 25 kDa size. Control used is culture grown to saturation in PG medium. No expression is seen in control. Lane L: Protein marker; Lane C: Control; Lane 45 h: Sample processed 45 hr post incubation at 25°C.

    Article Snippet: For immunodetection of the His fusion proteins, the primary antibody used was His probe (H-3) mouse monoclonal IgG (1 : 500; Santa Cruz Biotechnology, USA) and the secondary antibody was HRP-conjugated anti-mouse IgG antibody (1 : 2000; Santa Cruz Biotechnology, USA).

    Techniques: Expressing, Recombinant, Staining, SDS Page, Incubation, Marker, Western Blot, Blocking Assay

    BtaE localization. (A and C) Western blot analysis of whole-membrane fractions from B. suis (A) and E. coli pBBR btaE (C) was carried out. Membrane samples were submitted to a strong denaturing process. Samples were electrophoresed by SDS-PAGE and transferred to a PDVF membrane. Blots were incubated with anti-BtaE antisera and with anti-mouse HRP-conjugated secondary antibody and finally revealed using ECL Plus. On the right, a Coomassie blue pattern is shown as a loading control (ctrl). (B and D) Detection of BtaE (in red) on the B. suis surface (B) and on the E. coli pBBR btaE surface (D) by immunofluorescence of GFP-tagged bacteria. Cultures of GFP-labeled strains were fixed, incubated with anti-BtaE antibodies, and then probed with a CY3-conjugated donkey anti-mouse antibody preparation. Samples were observed with a Plan-Aprochromat 100×/1.4 oil DIC objective on a Zeiss LSM 5 Pascal confocal microscope.

    Journal: Infection and Immunity

    Article Title: BtaE, an Adhesin That Belongs to the Trimeric Autotransporter Family, Is Required for Full Virulence and Defines a Specific Adhesive Pole of Brucella suis

    doi: 10.1128/IAI.01241-12

    Figure Lengend Snippet: BtaE localization. (A and C) Western blot analysis of whole-membrane fractions from B. suis (A) and E. coli pBBR btaE (C) was carried out. Membrane samples were submitted to a strong denaturing process. Samples were electrophoresed by SDS-PAGE and transferred to a PDVF membrane. Blots were incubated with anti-BtaE antisera and with anti-mouse HRP-conjugated secondary antibody and finally revealed using ECL Plus. On the right, a Coomassie blue pattern is shown as a loading control (ctrl). (B and D) Detection of BtaE (in red) on the B. suis surface (B) and on the E. coli pBBR btaE surface (D) by immunofluorescence of GFP-tagged bacteria. Cultures of GFP-labeled strains were fixed, incubated with anti-BtaE antibodies, and then probed with a CY3-conjugated donkey anti-mouse antibody preparation. Samples were observed with a Plan-Aprochromat 100×/1.4 oil DIC objective on a Zeiss LSM 5 Pascal confocal microscope.

    Article Snippet: Blots were probed with polyclonal mouse anti-BtaE serum (1:8,000) and a goat horseradish peroxidase (HRP)-conjugated secondary anti-mouse antibody (1:30,000; Santa Cruz) and then revealed using ECL Plus (Amersham).

    Techniques: Western Blot, SDS Page, Incubation, Immunofluorescence, Labeling, Microscopy