horseradish peroxidase conjugated secondary antibody  (Roche)

 
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    Structured Review

    Roche horseradish peroxidase conjugated secondary antibody
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibody/product/Roche
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
    92/100 stars

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    Related Articles

    Western Blot:

    Article Title: Inhibition of soluble epoxide hydrolase prevents diabetic retinopathy
    Article Snippet: .. Membranes were blocked in 3% BSA in PBS, incubated with primary and horseradish peroxidase-conjugated secondary antibodies in blocking solution, and detection was performed with a Lumi-Light plus western blotting substrate (Roche, Mannheim, Germany). .. For detection using the LI-COR system, after incubating with primary antibodies, the membranes were incubated for 1 hour with IRDye800CW- and IRDye680-conjugated secondary antibodies (LI-COR Biosciences, Bad Homburg, Germany).

    Incubation:

    Article Title: Identification and functional characterization of Polo-like kinase 2 autoregulatory sites
    Article Snippet: .. Blots were incubated with primary antibodies overnight at 4°C and Horseradish peroxidase-conjugated secondary antibodies (Roche) for 1 hr at room temperature. .. Blots were imaged through enhanced chemiluminescence system (Western Lightning (Perkin Elmer) or SuperSignal West Femto (Pierce)).

    Article Title: Interfering histone deacetylase 4 inhibits the proliferation of vascular smooth muscle cells via regulating MEG3/miR-125a-5p/IRF1
    Article Snippet: .. After primary antibody incubation, the membranes were incubated with the corresponding horseradish peroxidase-conjugated secondary antibody at room temperature for 1 h. The enhanced chemiluminescence system (ECL, Roche Molecular Biochemicals) was used to detect protein-antibody complexes. β-actin was served as a positive control to quantify the expression of related proteins. .. CCK-8 assay VSMCs proliferation was measured using CCK-8 Assay Kit (Solarbio, USA) according to the manufacturer’s instructions.

    Article Title: Inhibition of soluble epoxide hydrolase prevents diabetic retinopathy
    Article Snippet: .. Membranes were blocked in 3% BSA in PBS, incubated with primary and horseradish peroxidase-conjugated secondary antibodies in blocking solution, and detection was performed with a Lumi-Light plus western blotting substrate (Roche, Mannheim, Germany). .. For detection using the LI-COR system, after incubating with primary antibodies, the membranes were incubated for 1 hour with IRDye800CW- and IRDye680-conjugated secondary antibodies (LI-COR Biosciences, Bad Homburg, Germany).

    Positive Control:

    Article Title: Interfering histone deacetylase 4 inhibits the proliferation of vascular smooth muscle cells via regulating MEG3/miR-125a-5p/IRF1
    Article Snippet: .. After primary antibody incubation, the membranes were incubated with the corresponding horseradish peroxidase-conjugated secondary antibody at room temperature for 1 h. The enhanced chemiluminescence system (ECL, Roche Molecular Biochemicals) was used to detect protein-antibody complexes. β-actin was served as a positive control to quantify the expression of related proteins. .. CCK-8 assay VSMCs proliferation was measured using CCK-8 Assay Kit (Solarbio, USA) according to the manufacturer’s instructions.

    Expressing:

    Article Title: Interfering histone deacetylase 4 inhibits the proliferation of vascular smooth muscle cells via regulating MEG3/miR-125a-5p/IRF1
    Article Snippet: .. After primary antibody incubation, the membranes were incubated with the corresponding horseradish peroxidase-conjugated secondary antibody at room temperature for 1 h. The enhanced chemiluminescence system (ECL, Roche Molecular Biochemicals) was used to detect protein-antibody complexes. β-actin was served as a positive control to quantify the expression of related proteins. .. CCK-8 assay VSMCs proliferation was measured using CCK-8 Assay Kit (Solarbio, USA) according to the manufacturer’s instructions.

    Blocking Assay:

    Article Title: Inhibition of soluble epoxide hydrolase prevents diabetic retinopathy
    Article Snippet: .. Membranes were blocked in 3% BSA in PBS, incubated with primary and horseradish peroxidase-conjugated secondary antibodies in blocking solution, and detection was performed with a Lumi-Light plus western blotting substrate (Roche, Mannheim, Germany). .. For detection using the LI-COR system, after incubating with primary antibodies, the membranes were incubated for 1 hour with IRDye800CW- and IRDye680-conjugated secondary antibodies (LI-COR Biosciences, Bad Homburg, Germany).

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  • 88
    Roche secondary horseradish peroxidase conjugated abs
    A comparison of the sensitivity of several assays. ( A ) IDAT assay in which lysates (1 mg/ml) from T6–17 or NR6 (p185 her2/neu negative) cells were diluted as indicated and captured by the <t>anti-p185</t> her2/neu Ab 1E1. The <t>ds-oligo-conjugated</t> mAb 4D5 was used to detect the antigen and perform the amplification assay. Although we detected a positive band in T6–19 cells at 10 −13 dilution, no positive band was noticeable in the control NR6 cells at 10 −3 dilution. It should be noted that this type of RNA amplification can lead to smaller-sized signals because of premature T7 termination and RNA degradation. Such smaller-sized signals are apparent in some figures. ( B ) A conventional sandwich ELISA assay with 1E1 and 4D5 was performed on cell lysates. An anti-human <t>IgG-horseradish</t> <t>peroxidase</t> (Zymed) was used as the <t>secondary</t> Ab. Results from NR6 cell lysates also were included to show the baseline of the ELISA assay. The ELISA assay detects p185 her2/neu readily only at 10 −4 , 10 −3 , 10 −2 , 10 −1 , and 10 0 dilutions. ( C ) Western blot assay. T6–17 cell lysate (lane 1, 10 μl; lane 2, 1 μl; lane 3, 0.1 μl, equal to 10 −1 , 10 −2 , and 10 −3 dilutions, respectively, as in A and B ) were loaded for 6% PAGE. A polyclonal anti-p185 her2/neu Ab NEU from Santa Cruz (200 μg/ml) was used for Western blot at 1:2,000 dilution.
    Secondary Horseradish Peroxidase Conjugated Abs, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/secondary horseradish peroxidase conjugated abs/product/Roche
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    secondary horseradish peroxidase conjugated abs - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    88
    Roche horseradish peroxidase conjugated secondary igg
    A comparison of the sensitivity of several assays. ( A ) IDAT assay in which lysates (1 mg/ml) from T6–17 or NR6 (p185 her2/neu negative) cells were diluted as indicated and captured by the <t>anti-p185</t> her2/neu Ab 1E1. The <t>ds-oligo-conjugated</t> mAb 4D5 was used to detect the antigen and perform the amplification assay. Although we detected a positive band in T6–19 cells at 10 −13 dilution, no positive band was noticeable in the control NR6 cells at 10 −3 dilution. It should be noted that this type of RNA amplification can lead to smaller-sized signals because of premature T7 termination and RNA degradation. Such smaller-sized signals are apparent in some figures. ( B ) A conventional sandwich ELISA assay with 1E1 and 4D5 was performed on cell lysates. An anti-human <t>IgG-horseradish</t> <t>peroxidase</t> (Zymed) was used as the <t>secondary</t> Ab. Results from NR6 cell lysates also were included to show the baseline of the ELISA assay. The ELISA assay detects p185 her2/neu readily only at 10 −4 , 10 −3 , 10 −2 , 10 −1 , and 10 0 dilutions. ( C ) Western blot assay. T6–17 cell lysate (lane 1, 10 μl; lane 2, 1 μl; lane 3, 0.1 μl, equal to 10 −1 , 10 −2 , and 10 −3 dilutions, respectively, as in A and B ) were loaded for 6% PAGE. A polyclonal anti-p185 her2/neu Ab NEU from Santa Cruz (200 μg/ml) was used for Western blot at 1:2,000 dilution.
    Horseradish Peroxidase Conjugated Secondary Igg, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary igg/product/Roche
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary igg - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    94
    Roche horseradish peroxidase
    A comparison of the sensitivity of several assays. ( A ) IDAT assay in which lysates (1 mg/ml) from T6–17 or NR6 (p185 her2/neu negative) cells were diluted as indicated and captured by the <t>anti-p185</t> her2/neu Ab 1E1. The <t>ds-oligo-conjugated</t> mAb 4D5 was used to detect the antigen and perform the amplification assay. Although we detected a positive band in T6–19 cells at 10 −13 dilution, no positive band was noticeable in the control NR6 cells at 10 −3 dilution. It should be noted that this type of RNA amplification can lead to smaller-sized signals because of premature T7 termination and RNA degradation. Such smaller-sized signals are apparent in some figures. ( B ) A conventional sandwich ELISA assay with 1E1 and 4D5 was performed on cell lysates. An anti-human <t>IgG-horseradish</t> <t>peroxidase</t> (Zymed) was used as the <t>secondary</t> Ab. Results from NR6 cell lysates also were included to show the baseline of the ELISA assay. The ELISA assay detects p185 her2/neu readily only at 10 −4 , 10 −3 , 10 −2 , 10 −1 , and 10 0 dilutions. ( C ) Western blot assay. T6–17 cell lysate (lane 1, 10 μl; lane 2, 1 μl; lane 3, 0.1 μl, equal to 10 −1 , 10 −2 , and 10 −3 dilutions, respectively, as in A and B ) were loaded for 6% PAGE. A polyclonal anti-p185 her2/neu Ab NEU from Santa Cruz (200 μg/ml) was used for Western blot at 1:2,000 dilution.
    Horseradish Peroxidase, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase/product/Roche
    Average 94 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    92
    Roche horseradish peroxidase hrp conjugated secondary antibodies
    A comparison of the sensitivity of several assays. ( A ) IDAT assay in which lysates (1 mg/ml) from T6–17 or NR6 (p185 her2/neu negative) cells were diluted as indicated and captured by the <t>anti-p185</t> her2/neu Ab 1E1. The <t>ds-oligo-conjugated</t> mAb 4D5 was used to detect the antigen and perform the amplification assay. Although we detected a positive band in T6–19 cells at 10 −13 dilution, no positive band was noticeable in the control NR6 cells at 10 −3 dilution. It should be noted that this type of RNA amplification can lead to smaller-sized signals because of premature T7 termination and RNA degradation. Such smaller-sized signals are apparent in some figures. ( B ) A conventional sandwich ELISA assay with 1E1 and 4D5 was performed on cell lysates. An anti-human <t>IgG-horseradish</t> <t>peroxidase</t> (Zymed) was used as the <t>secondary</t> Ab. Results from NR6 cell lysates also were included to show the baseline of the ELISA assay. The ELISA assay detects p185 her2/neu readily only at 10 −4 , 10 −3 , 10 −2 , 10 −1 , and 10 0 dilutions. ( C ) Western blot assay. T6–17 cell lysate (lane 1, 10 μl; lane 2, 1 μl; lane 3, 0.1 μl, equal to 10 −1 , 10 −2 , and 10 −3 dilutions, respectively, as in A and B ) were loaded for 6% PAGE. A polyclonal anti-p185 her2/neu Ab NEU from Santa Cruz (200 μg/ml) was used for Western blot at 1:2,000 dilution.
    Horseradish Peroxidase Hrp Conjugated Secondary Antibodies, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated secondary antibodies/product/Roche
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    A comparison of the sensitivity of several assays. ( A ) IDAT assay in which lysates (1 mg/ml) from T6–17 or NR6 (p185 her2/neu negative) cells were diluted as indicated and captured by the anti-p185 her2/neu Ab 1E1. The ds-oligo-conjugated mAb 4D5 was used to detect the antigen and perform the amplification assay. Although we detected a positive band in T6–19 cells at 10 −13 dilution, no positive band was noticeable in the control NR6 cells at 10 −3 dilution. It should be noted that this type of RNA amplification can lead to smaller-sized signals because of premature T7 termination and RNA degradation. Such smaller-sized signals are apparent in some figures. ( B ) A conventional sandwich ELISA assay with 1E1 and 4D5 was performed on cell lysates. An anti-human IgG-horseradish peroxidase (Zymed) was used as the secondary Ab. Results from NR6 cell lysates also were included to show the baseline of the ELISA assay. The ELISA assay detects p185 her2/neu readily only at 10 −4 , 10 −3 , 10 −2 , 10 −1 , and 10 0 dilutions. ( C ) Western blot assay. T6–17 cell lysate (lane 1, 10 μl; lane 2, 1 μl; lane 3, 0.1 μl, equal to 10 −1 , 10 −2 , and 10 −3 dilutions, respectively, as in A and B ) were loaded for 6% PAGE. A polyclonal anti-p185 her2/neu Ab NEU from Santa Cruz (200 μg/ml) was used for Western blot at 1:2,000 dilution.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Protein quantification from complex protein mixtures using a proteomics methodology with single-cell resolution

    doi: 10.1073/pnas.101124598

    Figure Lengend Snippet: A comparison of the sensitivity of several assays. ( A ) IDAT assay in which lysates (1 mg/ml) from T6–17 or NR6 (p185 her2/neu negative) cells were diluted as indicated and captured by the anti-p185 her2/neu Ab 1E1. The ds-oligo-conjugated mAb 4D5 was used to detect the antigen and perform the amplification assay. Although we detected a positive band in T6–19 cells at 10 −13 dilution, no positive band was noticeable in the control NR6 cells at 10 −3 dilution. It should be noted that this type of RNA amplification can lead to smaller-sized signals because of premature T7 termination and RNA degradation. Such smaller-sized signals are apparent in some figures. ( B ) A conventional sandwich ELISA assay with 1E1 and 4D5 was performed on cell lysates. An anti-human IgG-horseradish peroxidase (Zymed) was used as the secondary Ab. Results from NR6 cell lysates also were included to show the baseline of the ELISA assay. The ELISA assay detects p185 her2/neu readily only at 10 −4 , 10 −3 , 10 −2 , 10 −1 , and 10 0 dilutions. ( C ) Western blot assay. T6–17 cell lysate (lane 1, 10 μl; lane 2, 1 μl; lane 3, 0.1 μl, equal to 10 −1 , 10 −2 , and 10 −3 dilutions, respectively, as in A and B ) were loaded for 6% PAGE. A polyclonal anti-p185 her2/neu Ab NEU from Santa Cruz (200 μg/ml) was used for Western blot at 1:2,000 dilution.

    Article Snippet: All polyclonal sera and secondary horseradish peroxidase-conjugated Abs (Roche Molecular Biochemicals) were used at a 1:5,000 dilution.

    Techniques: Amplification, Sandwich ELISA, Enzyme-linked Immunosorbent Assay, Western Blot, Polyacrylamide Gel Electrophoresis