horseradish peroxidase conjugated secondary antibody  (Proteintech)

 
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    Proteintech horseradish peroxidase conjugated secondary antibody
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibody/product/Proteintech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
    92/100 stars

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    Incubation:

    Article Title: S100A4 over-expression underlies lymph node metastasis and poor prognosis in colorectal cancer
    Article Snippet: .. The membranes were incubated with rabbit polyclonal antibody against S100A4 (1:1000 dilution; Abcam, Cambridge, UK) and then incubated with a horseradish-peroxidase-conjugated secondary antibody (1:100 dilution; Proteintech, Chicago, IL, USA). β-actin was detected simultaneously as a loading control (anti-β-actin, 1:1000 dilution; Kangchen, Beijing, China). .. All blots were visualized using an ECL detection system (Amersham, Arlington Heights, IL, USA )and quantitated by densitometry using an LAS-3000 imager.

    Article Title: Correlation between mitochondrial TRAP-1 expression and lymph node metastasis in colorectal cancer
    Article Snippet: .. The membranes were incubated with rabbit polyclonal antibody against TRAP-1 (1:1000 dilution; Abcam, Cambridge, United Kingdom) and then incubated with a horseradish-peroxidase-conjugated secondary antibody (1:100 dilution; Proteintech, Chicago, IL, United States). β-actin was detected simultaneously as a loading control (anti-β-actin, 1:1000 dilution; Kangchen, Beijing, China). .. All blots were visualized using an ECL detection system (Amersham, Arlington Heights, IL, United States) and quantitated by densitometry using an LAS-3000 imager.

    Article Title: Activation of the RLR/MAVS Signaling Pathway by the L Protein of Mopeia Virus
    Article Snippet: .. After blocking with Tris-buffered saline–Tween 20 buffer (TBST) containing 5% nonfat milk, the membranes were incubated with primary antibodies and the corresponding horseradish peroxidase-conjugated secondary antibodies (ProteinTech, Wuhan, China). .. Protein bands were detected by enhanced chemiluminescence (ECL) (Millipore, Billerica, MA).

    Blocking Assay:

    Article Title: Activation of the RLR/MAVS Signaling Pathway by the L Protein of Mopeia Virus
    Article Snippet: .. After blocking with Tris-buffered saline–Tween 20 buffer (TBST) containing 5% nonfat milk, the membranes were incubated with primary antibodies and the corresponding horseradish peroxidase-conjugated secondary antibodies (ProteinTech, Wuhan, China). .. Protein bands were detected by enhanced chemiluminescence (ECL) (Millipore, Billerica, MA).

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    Proteintech rabbit anti rat igg hrp conjugated secondary antibody
    Antibody titers of <t>IgG</t> induced by Cs Pmy measured by ELISA. Briefly, 1 µg/well recombinant pET-26b- Cs Pmy protein was coated on the plates and blocked with 5% skimmed milk. The plate was incubated with different dilutions of the immune sera (week 6) raised by pET-26b- Cs Pmy and pcDNA- Cs Pmy. Rat sera immunized with PBS and pcDNA were measured under the same conditions as negative controls. <t>HRP-conjugated</t> IgG (1∶20000 dilutions) was used as the secondary antibodies. The reactions were developed with substrate solution TMB, stopped by 2 M H 2 SO4 and measured at measured at 450 nm. (A) Antibody titers of IgG induced by pET-26b- Cs Pmy. (B) Antibody titers of IgG induced by pcDNA- Cs Pmy.
    Rabbit Anti Rat Igg Hrp Conjugated Secondary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti rat igg hrp conjugated secondary antibody/product/Proteintech
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rabbit anti rat igg hrp conjugated secondary antibody - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    93
    Proteintech horseradish peroxidase conjugated affinipure goat anti rabbit igg
    Antibody titers of <t>IgG</t> induced by Cs Pmy measured by ELISA. Briefly, 1 µg/well recombinant pET-26b- Cs Pmy protein was coated on the plates and blocked with 5% skimmed milk. The plate was incubated with different dilutions of the immune sera (week 6) raised by pET-26b- Cs Pmy and pcDNA- Cs Pmy. Rat sera immunized with PBS and pcDNA were measured under the same conditions as negative controls. <t>HRP-conjugated</t> IgG (1∶20000 dilutions) was used as the secondary antibodies. The reactions were developed with substrate solution TMB, stopped by 2 M H 2 SO4 and measured at measured at 450 nm. (A) Antibody titers of IgG induced by pET-26b- Cs Pmy. (B) Antibody titers of IgG induced by pcDNA- Cs Pmy.
    Horseradish Peroxidase Conjugated Affinipure Goat Anti Rabbit Igg, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated affinipure goat anti rabbit igg/product/Proteintech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated affinipure goat anti rabbit igg - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    91
    Proteintech horseradish peroxidase hrp conjugated secondary anti rabbit
    Antibody titers of <t>IgG</t> induced by Cs Pmy measured by ELISA. Briefly, 1 µg/well recombinant pET-26b- Cs Pmy protein was coated on the plates and blocked with 5% skimmed milk. The plate was incubated with different dilutions of the immune sera (week 6) raised by pET-26b- Cs Pmy and pcDNA- Cs Pmy. Rat sera immunized with PBS and pcDNA were measured under the same conditions as negative controls. <t>HRP-conjugated</t> IgG (1∶20000 dilutions) was used as the secondary antibodies. The reactions were developed with substrate solution TMB, stopped by 2 M H 2 SO4 and measured at measured at 450 nm. (A) Antibody titers of IgG induced by pET-26b- Cs Pmy. (B) Antibody titers of IgG induced by pcDNA- Cs Pmy.
    Horseradish Peroxidase Hrp Conjugated Secondary Anti Rabbit, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated secondary anti rabbit/product/Proteintech
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated secondary anti rabbit - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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    Antibody titers of IgG induced by Cs Pmy measured by ELISA. Briefly, 1 µg/well recombinant pET-26b- Cs Pmy protein was coated on the plates and blocked with 5% skimmed milk. The plate was incubated with different dilutions of the immune sera (week 6) raised by pET-26b- Cs Pmy and pcDNA- Cs Pmy. Rat sera immunized with PBS and pcDNA were measured under the same conditions as negative controls. HRP-conjugated IgG (1∶20000 dilutions) was used as the secondary antibodies. The reactions were developed with substrate solution TMB, stopped by 2 M H 2 SO4 and measured at measured at 450 nm. (A) Antibody titers of IgG induced by pET-26b- Cs Pmy. (B) Antibody titers of IgG induced by pcDNA- Cs Pmy.

    Journal: PLoS ONE

    Article Title: Identification and Characterization of Paramyosin from Cyst Wall of Metacercariae Implicated Protective Efficacy against Clonorchis sinensis Infection

    doi: 10.1371/journal.pone.0033703

    Figure Lengend Snippet: Antibody titers of IgG induced by Cs Pmy measured by ELISA. Briefly, 1 µg/well recombinant pET-26b- Cs Pmy protein was coated on the plates and blocked with 5% skimmed milk. The plate was incubated with different dilutions of the immune sera (week 6) raised by pET-26b- Cs Pmy and pcDNA- Cs Pmy. Rat sera immunized with PBS and pcDNA were measured under the same conditions as negative controls. HRP-conjugated IgG (1∶20000 dilutions) was used as the secondary antibodies. The reactions were developed with substrate solution TMB, stopped by 2 M H 2 SO4 and measured at measured at 450 nm. (A) Antibody titers of IgG induced by pET-26b- Cs Pmy. (B) Antibody titers of IgG induced by pcDNA- Cs Pmy.

    Article Snippet: Subsequently, the membrane was followed by incubation with rabbit anti-rat IgG HRP-conjugated secondary antibody (1∶2000 dilutions in 1% BSA-PBS, Proteintech Group, Chicago, USA) at room temperature for 1 h. The protein bands were visualized by enhanced chemiluminescence (ECL) method.

    Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Positron Emission Tomography, Incubation

    Identification of Cs Pmy by SDS-PAGE and Western blot analysis. Protein molecular weight marker (M), purified pET-26b- Cs Pmy protein (lane 1), TWE of adult worm (lane 2), TWE of metacercaria (lane 3), TWE of cercaria (lane 4), TWE of egg (lane 5), cyst wall proteins of metacercaria (lane 6), and soluble tegumental components of adult worm (lane 7). (A) 8% SDS-PAGE. (B) Western blot analysis. Corresponding proteins were subjected to 8% SDS-PAGE and immobilized onto the membrane, then the membrane was incubated with anti-pET-26b- Cs Pmy rat serum (1∶2000 dilutions) at room temperature for 2 h. Subsequently, the membrane was followed by incubation with rabbit anti-rat IgG HRP-conjugated secondary antibody (1∶2000 dilutions) at room temperature for 1 h. 2 µg of purified pET-26b- Cs Pmy protein and 10 µg of TWE were loaded per lane. SDS-PAGE was visualized by Coomassie Blues staining and the protein bands that might be native paramyosin in different life stages were indicated with arrows. Western blot was visualized by ECL method, the detected protein bands were around 100 kDa.

    Journal: PLoS ONE

    Article Title: Identification and Characterization of Paramyosin from Cyst Wall of Metacercariae Implicated Protective Efficacy against Clonorchis sinensis Infection

    doi: 10.1371/journal.pone.0033703

    Figure Lengend Snippet: Identification of Cs Pmy by SDS-PAGE and Western blot analysis. Protein molecular weight marker (M), purified pET-26b- Cs Pmy protein (lane 1), TWE of adult worm (lane 2), TWE of metacercaria (lane 3), TWE of cercaria (lane 4), TWE of egg (lane 5), cyst wall proteins of metacercaria (lane 6), and soluble tegumental components of adult worm (lane 7). (A) 8% SDS-PAGE. (B) Western blot analysis. Corresponding proteins were subjected to 8% SDS-PAGE and immobilized onto the membrane, then the membrane was incubated with anti-pET-26b- Cs Pmy rat serum (1∶2000 dilutions) at room temperature for 2 h. Subsequently, the membrane was followed by incubation with rabbit anti-rat IgG HRP-conjugated secondary antibody (1∶2000 dilutions) at room temperature for 1 h. 2 µg of purified pET-26b- Cs Pmy protein and 10 µg of TWE were loaded per lane. SDS-PAGE was visualized by Coomassie Blues staining and the protein bands that might be native paramyosin in different life stages were indicated with arrows. Western blot was visualized by ECL method, the detected protein bands were around 100 kDa.

    Article Snippet: Subsequently, the membrane was followed by incubation with rabbit anti-rat IgG HRP-conjugated secondary antibody (1∶2000 dilutions in 1% BSA-PBS, Proteintech Group, Chicago, USA) at room temperature for 1 h. The protein bands were visualized by enhanced chemiluminescence (ECL) method.

    Techniques: SDS Page, Western Blot, Molecular Weight, Marker, Purification, Positron Emission Tomography, Incubation, Staining