horseradish peroxidase conjugated secondary antibody  (Agilent technologies)

 
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    Structured Review

    Agilent technologies horseradish peroxidase conjugated secondary antibody
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibody/product/Agilent technologies
    Average 94 stars, based on 117 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Collar-induced elevation of mRNA and functional activity of 5-HT1B receptor in the rabbit carotid artery
    Article Snippet: .. After washing with Tween-TBS and incubation with the appropriate horseradish peroxidase-conjugated secondary antibody (rabbit anti goat IgG (1 : 1500, DAKO, Glastrup, Denmark) or goat anti-mouse IgG (1 : 400, Jackson ImmunoResearch Laboratories, Westmore, PA, U.S.A.)) for 1 h at room temperature, the bound antibodies were detected using the enhanced chemiluminescent system (ECL™ Western blotting detection reagents and Hyperfilm™ ECL™, Amersham, Gent, Belgium). .. After washing the membranes with Tween-TBS, the membranes were blocked again with 10% (w v−1 ) non-fat dry milk powder in Tween-TBS and the whole immunodetection procedure was repeated.

    Article Title: Triple negative tumors accumulate significantly less methylglyoxal specific adducts than other human breast cancer subtypes
    Article Snippet: .. Horseradish peroxidase-conjugated secondary antibodies [anti-mouse, 1:6,000 dilution (Dako) or anti-rabbit 1:3,000 (Invitrogen)] were used to visualize bound primary antibodies, with the ECL Western blotting substrate (Pierce). .. Where indicated, ImageJ software 1.46r ( imagej.nih.gov ) was used for semi-quantitation using beta-actin as a loading control.

    Article Title: ORF9p Phosphorylation by ORF47p Is Crucial for the Formation and Egress of Varicella-Zoster Virus Viral Particles
    Article Snippet: .. Horseradish peroxidase-conjugated secondary antibodies for Western blotting were obtained from Dako (number P044701, number P039901) and Jackson Immuno Research (number 211-032-171, number 115-035-174). .. Alexa 633 anti-mouse ( ) and Alexa 568 anti-rabbit ( ) secondary antibodies for immunofluorescence were obtained from Invitrogen.

    Incubation:

    Article Title: Collar-induced elevation of mRNA and functional activity of 5-HT1B receptor in the rabbit carotid artery
    Article Snippet: .. After washing with Tween-TBS and incubation with the appropriate horseradish peroxidase-conjugated secondary antibody (rabbit anti goat IgG (1 : 1500, DAKO, Glastrup, Denmark) or goat anti-mouse IgG (1 : 400, Jackson ImmunoResearch Laboratories, Westmore, PA, U.S.A.)) for 1 h at room temperature, the bound antibodies were detected using the enhanced chemiluminescent system (ECL™ Western blotting detection reagents and Hyperfilm™ ECL™, Amersham, Gent, Belgium). .. After washing the membranes with Tween-TBS, the membranes were blocked again with 10% (w v−1 ) non-fat dry milk powder in Tween-TBS and the whole immunodetection procedure was repeated.

    Article Title: miR-193a-5p promotes pancreatic cancer cell metastasis through SRSF6-mediated alternative splicing of OGDHL and ECM1
    Article Snippet: .. Horseradish peroxidase-conjugated secondary antibody (Dako North America Inc., Carpinteria, CA, USA) was added dropwise into sections, and were then incubated at room temperature for 30 min. .. The incubated sections were stained by diaminobenzidine (DAB), and then counterstained by hematoxylin, before being mounted on slides with neutral balsam.

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  • 91
    Agilent technologies envision plus polymer anti mouse conjugated to hrp
    Representative biopsies of pediatric duodenum immune-stained for αS . Upper panels: The three top panels are serial sections (20X), stained with H E, immuno-stained for αS, or for CD68. The specimen exhibits severe acute and chronic inflammation. Lower panels: αS co-localizes with PGP 9.5 by double immunofluorescence demonstrating expression within neurons. is shown. Primary antibodies: α-syn: ab27766 from Abcam; PGP 9.5: Z5116 from DAKO. Secondary antibodies: α-syn: Envision anti-mouse <t>HRP</t> polymer from <t>Agilent;</t> PGP 9.5: Goat anti-rabbit biotin conjugated from Vector. Tertiary (fluorescent tag): α-syn: Perkin Elmer Tyramide Signal Amplification with Cy5; PGP 9.5: Life Technologies streptavidin conjugated with AlexaFluor488. Procedures were as specified by the protocols provided with the reagents. Cy5 was imaged at 555nm, GFP at 488nm. Images were taken using Slidebook 6.0 and the overlay was an output from the Slidebook System.
    Envision Plus Polymer Anti Mouse Conjugated To Hrp, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/envision plus polymer anti mouse conjugated to hrp/product/Agilent technologies
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    envision plus polymer anti mouse conjugated to hrp - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    91
    Agilent technologies appropriate hrp conjugated secondary antibodies
    Representative biopsies of pediatric duodenum immune-stained for αS . Upper panels: The three top panels are serial sections (20X), stained with H E, immuno-stained for αS, or for CD68. The specimen exhibits severe acute and chronic inflammation. Lower panels: αS co-localizes with PGP 9.5 by double immunofluorescence demonstrating expression within neurons. is shown. Primary antibodies: α-syn: ab27766 from Abcam; PGP 9.5: Z5116 from DAKO. Secondary antibodies: α-syn: Envision anti-mouse <t>HRP</t> polymer from <t>Agilent;</t> PGP 9.5: Goat anti-rabbit biotin conjugated from Vector. Tertiary (fluorescent tag): α-syn: Perkin Elmer Tyramide Signal Amplification with Cy5; PGP 9.5: Life Technologies streptavidin conjugated with AlexaFluor488. Procedures were as specified by the protocols provided with the reagents. Cy5 was imaged at 555nm, GFP at 488nm. Images were taken using Slidebook 6.0 and the overlay was an output from the Slidebook System.
    Appropriate Hrp Conjugated Secondary Antibodies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/appropriate hrp conjugated secondary antibodies/product/Agilent technologies
    Average 91 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    appropriate hrp conjugated secondary antibodies - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    94
    Agilent technologies horseradish peroxidase
    Representative biopsies of pediatric duodenum immune-stained for αS . Upper panels: The three top panels are serial sections (20X), stained with H E, immuno-stained for αS, or for CD68. The specimen exhibits severe acute and chronic inflammation. Lower panels: αS co-localizes with PGP 9.5 by double immunofluorescence demonstrating expression within neurons. is shown. Primary antibodies: α-syn: ab27766 from Abcam; PGP 9.5: Z5116 from DAKO. Secondary antibodies: α-syn: Envision anti-mouse <t>HRP</t> polymer from <t>Agilent;</t> PGP 9.5: Goat anti-rabbit biotin conjugated from Vector. Tertiary (fluorescent tag): α-syn: Perkin Elmer Tyramide Signal Amplification with Cy5; PGP 9.5: Life Technologies streptavidin conjugated with AlexaFluor488. Procedures were as specified by the protocols provided with the reagents. Cy5 was imaged at 555nm, GFP at 488nm. Images were taken using Slidebook 6.0 and the overlay was an output from the Slidebook System.
    Horseradish Peroxidase, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase/product/Agilent technologies
    Average 94 stars, based on 510 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Representative biopsies of pediatric duodenum immune-stained for αS . Upper panels: The three top panels are serial sections (20X), stained with H E, immuno-stained for αS, or for CD68. The specimen exhibits severe acute and chronic inflammation. Lower panels: αS co-localizes with PGP 9.5 by double immunofluorescence demonstrating expression within neurons. is shown. Primary antibodies: α-syn: ab27766 from Abcam; PGP 9.5: Z5116 from DAKO. Secondary antibodies: α-syn: Envision anti-mouse HRP polymer from Agilent; PGP 9.5: Goat anti-rabbit biotin conjugated from Vector. Tertiary (fluorescent tag): α-syn: Perkin Elmer Tyramide Signal Amplification with Cy5; PGP 9.5: Life Technologies streptavidin conjugated with AlexaFluor488. Procedures were as specified by the protocols provided with the reagents. Cy5 was imaged at 555nm, GFP at 488nm. Images were taken using Slidebook 6.0 and the overlay was an output from the Slidebook System.

    Journal: Journal of innate immunity

    Article Title: A role for neuronal alpha synuclein in gastrointestinal immunity

    doi: 10.1159/000477990

    Figure Lengend Snippet: Representative biopsies of pediatric duodenum immune-stained for αS . Upper panels: The three top panels are serial sections (20X), stained with H E, immuno-stained for αS, or for CD68. The specimen exhibits severe acute and chronic inflammation. Lower panels: αS co-localizes with PGP 9.5 by double immunofluorescence demonstrating expression within neurons. is shown. Primary antibodies: α-syn: ab27766 from Abcam; PGP 9.5: Z5116 from DAKO. Secondary antibodies: α-syn: Envision anti-mouse HRP polymer from Agilent; PGP 9.5: Goat anti-rabbit biotin conjugated from Vector. Tertiary (fluorescent tag): α-syn: Perkin Elmer Tyramide Signal Amplification with Cy5; PGP 9.5: Life Technologies streptavidin conjugated with AlexaFluor488. Procedures were as specified by the protocols provided with the reagents. Cy5 was imaged at 555nm, GFP at 488nm. Images were taken using Slidebook 6.0 and the overlay was an output from the Slidebook System.

    Article Snippet: The secondary antibody was Envision Plus Polymer anti-mouse conjugated to HRP (Agilent K4001).

    Techniques: Staining, Immunofluorescence, Expressing, Plasmid Preparation, Amplification