horseradish peroxidase conjugated secondary antibody  (Abcam)

 
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    Structured Review

    Abcam horseradish peroxidase conjugated secondary antibody
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibody/product/Abcam
    Average 99 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibody - by Bioz Stars, 2022-09
    99/100 stars

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  • 93
    Abcam hrp conjugated anti alpha tubulin monoclonal antibody
    Expression analysis of Siglec-3, Siglec-5 and Siglec-10 using IFA and Western blotting. (a) PK-15 cells were transfected with Siglec-3-, Siglec-5- and Siglec-10-encoding vector. Twenty-four hours post-transfection, the cells were fixed with 4 % PF and permeabilized with Triton X-100 for cytoplasmic staining, or not permeabilized for surface staining. IFA was performed using V5-specific antibody (Siglec; green) and Hoechst 33 342 (nuclei; blue). Scale bar: 25 µm (b) Western blot identification. HEK-293T cells were transfected with Siglec-3, Siglec-5 and Siglec-10. Twenty-four hours post-transfection, the cells were collected and lysed with lysis buffer. Afterwards, cellular lysates were treated or mock-treated with sialidase, EndoH or PNGase F and analysed by SDS-PAGE and Western blot. Primary antibody V5-specific mAb (GenScript; A00641) and secondary peroxidase-labelled goat anti-mouse IgG antibodies (Dako) were used for immunoblotting. For the detection of <t>tubulin,</t> an <t>HRP-conjugated</t> <t>anti-alpha</t> tubulin monoclonal antibody (Abcam; ab40742) was used.
    Hrp Conjugated Anti Alpha Tubulin Monoclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated anti alpha tubulin monoclonal antibody/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated anti alpha tubulin monoclonal antibody - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

    97
    Abcam goat anti rabbit igg h l hrp
    Ablation of HTLV-1 CTCF-binding site significantly decreases HTLV-1-specific antibody response, but not total rabbit <t>IgG.</t> a Antibody response was quantified using a modified Avioq HTLV-1/2 Microelisa System protocol (Avioq, Inc., Research Triangle Park, NC). The supplied horseradish peroxidase <t>(HRP)</t> conjugated goat anti-human immunoglobulin (Ig) was substituted for an HRP conjugated goat anti-rabbit IgG (ab6721; Abcam, Cambridge, United Kingdom). Rabbit plasma was diluted 1:500 to obtain absorbance values within the linear range of the assay. Each symbol represents the absorbance value of a single inoculated rabbit at 0, 2, 4, 8, or 12 weeks post-infection within each group. b Total rabbit IgG was quantified using the Abcam Rabbit IgG ELISA Kit in accordance with the provided protocol (ab187400; Abcam, Cambridge, United Kingdom). Plasma samples were diluted 1:1 × 10 6 . Each symbol represents total IgG of a single inoculated rabbit at 0, 2, or 12 weeks post-infection within each group. Bars represent mean absorbance or IgG values. Mixed model analyses with a Bonferroni correction were performed in weeks 8 and 12 (HTLV-1-specific) or 2 and 12 (total rabbit IgG) to determine statistical significance. A p
    Goat Anti Rabbit Igg H L Hrp, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg h l hrp/product/Abcam
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit igg h l hrp - by Bioz Stars, 2022-09
    97/100 stars
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    99
    Abcam antibody conjugated to hrp
    Immunoblot analysis to confirm the expression of <t>EpCAM–IgM</t> Fc in representative T1 transgenic lines. ( A ) T1 transgenic plants #2–9, 2–14, 2–17, 2–21, 2–23, and 2–27 were tested by goat anti-human IgM Fc µ-chain conjugated to <t>HRP.</t> M, protein marker; +, tobacco plant EpCAM–IgM Fc as a positive control; −, non-transgenic plant as a negative control. ( B ) T1 transgenic plants #2–9, 2–14, 2–17, 2–21, 2–23, and 2–27 were tested by mouse anti-human EpCAM as the first antibody and goat anti-mouse IgG 2a conjugated to HRP as the second antibody. M, protein marker; +, tobacco plant EpCAM–IgM Fc as a positive control (50 ng); −, non-transgenic plant as a negative control; hygromycin phosphotransferase (HTP).
    Antibody Conjugated To Hrp, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody conjugated to hrp/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody conjugated to hrp - by Bioz Stars, 2022-09
    99/100 stars
      Buy from Supplier

    94
    Abcam horseradish peroxidase hrp conjugated anti glutathione s transferase gst antibodies
    Binding of the identified compounds to the p21-binding domain (PBD). ( a ) Binding sensorgram for compound 2 . PBD or <t>glutathione</t> S -transferase <t>(GST;</t> control) was immobilized on the gold surface, and then various concentrations of compound 2 were passed over the immobilized PBD or GST (RU: response unit). ( b ) The K d values for the compound–PBD interactions as measured by SPR. ( c ) Effect of a mutation in the CRIB motif on the interaction of PBD with compound 2 . Compound 2 (100 μ M ) was passed over the immobilized wild-type PBD (Wt-PBD) or the PBD with two mutations (H83L/H86L; PBD-LL). All data are representative of at least three independent experiments.
    Horseradish Peroxidase Hrp Conjugated Anti Glutathione S Transferase Gst Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated anti glutathione s transferase gst antibodies/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated anti glutathione s transferase gst antibodies - by Bioz Stars, 2022-09
    94/100 stars
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    Expression analysis of Siglec-3, Siglec-5 and Siglec-10 using IFA and Western blotting. (a) PK-15 cells were transfected with Siglec-3-, Siglec-5- and Siglec-10-encoding vector. Twenty-four hours post-transfection, the cells were fixed with 4 % PF and permeabilized with Triton X-100 for cytoplasmic staining, or not permeabilized for surface staining. IFA was performed using V5-specific antibody (Siglec; green) and Hoechst 33 342 (nuclei; blue). Scale bar: 25 µm (b) Western blot identification. HEK-293T cells were transfected with Siglec-3, Siglec-5 and Siglec-10. Twenty-four hours post-transfection, the cells were collected and lysed with lysis buffer. Afterwards, cellular lysates were treated or mock-treated with sialidase, EndoH or PNGase F and analysed by SDS-PAGE and Western blot. Primary antibody V5-specific mAb (GenScript; A00641) and secondary peroxidase-labelled goat anti-mouse IgG antibodies (Dako) were used for immunoblotting. For the detection of tubulin, an HRP-conjugated anti-alpha tubulin monoclonal antibody (Abcam; ab40742) was used.

    Journal: The Journal of General Virology

    Article Title: Molecular cloning of porcine Siglec-3, Siglec-5 and Siglec-10, and identification of Siglec-10 as an alternative receptor for porcine reproductive and respiratory syndrome virus (PRRSV)

    doi: 10.1099/jgv.0.000859

    Figure Lengend Snippet: Expression analysis of Siglec-3, Siglec-5 and Siglec-10 using IFA and Western blotting. (a) PK-15 cells were transfected with Siglec-3-, Siglec-5- and Siglec-10-encoding vector. Twenty-four hours post-transfection, the cells were fixed with 4 % PF and permeabilized with Triton X-100 for cytoplasmic staining, or not permeabilized for surface staining. IFA was performed using V5-specific antibody (Siglec; green) and Hoechst 33 342 (nuclei; blue). Scale bar: 25 µm (b) Western blot identification. HEK-293T cells were transfected with Siglec-3, Siglec-5 and Siglec-10. Twenty-four hours post-transfection, the cells were collected and lysed with lysis buffer. Afterwards, cellular lysates were treated or mock-treated with sialidase, EndoH or PNGase F and analysed by SDS-PAGE and Western blot. Primary antibody V5-specific mAb (GenScript; A00641) and secondary peroxidase-labelled goat anti-mouse IgG antibodies (Dako) were used for immunoblotting. For the detection of tubulin, an HRP-conjugated anti-alpha tubulin monoclonal antibody (Abcam; ab40742) was used.

    Article Snippet: HRP-conjugated anti-alpha tubulin monoclonal antibody (Abcam, ab40742) was used to stain tubulin.

    Techniques: Expressing, Immunofluorescence, Western Blot, Transfection, Plasmid Preparation, Staining, Lysis, SDS Page

    Ablation of HTLV-1 CTCF-binding site significantly decreases HTLV-1-specific antibody response, but not total rabbit IgG. a Antibody response was quantified using a modified Avioq HTLV-1/2 Microelisa System protocol (Avioq, Inc., Research Triangle Park, NC). The supplied horseradish peroxidase (HRP) conjugated goat anti-human immunoglobulin (Ig) was substituted for an HRP conjugated goat anti-rabbit IgG (ab6721; Abcam, Cambridge, United Kingdom). Rabbit plasma was diluted 1:500 to obtain absorbance values within the linear range of the assay. Each symbol represents the absorbance value of a single inoculated rabbit at 0, 2, 4, 8, or 12 weeks post-infection within each group. b Total rabbit IgG was quantified using the Abcam Rabbit IgG ELISA Kit in accordance with the provided protocol (ab187400; Abcam, Cambridge, United Kingdom). Plasma samples were diluted 1:1 × 10 6 . Each symbol represents total IgG of a single inoculated rabbit at 0, 2, or 12 weeks post-infection within each group. Bars represent mean absorbance or IgG values. Mixed model analyses with a Bonferroni correction were performed in weeks 8 and 12 (HTLV-1-specific) or 2 and 12 (total rabbit IgG) to determine statistical significance. A p

    Journal: Retrovirology

    Article Title: HTLV-1 CTCF-binding site is dispensable for in vitro immortalization and persistent infection in vivo

    doi: 10.1186/s12977-019-0507-9

    Figure Lengend Snippet: Ablation of HTLV-1 CTCF-binding site significantly decreases HTLV-1-specific antibody response, but not total rabbit IgG. a Antibody response was quantified using a modified Avioq HTLV-1/2 Microelisa System protocol (Avioq, Inc., Research Triangle Park, NC). The supplied horseradish peroxidase (HRP) conjugated goat anti-human immunoglobulin (Ig) was substituted for an HRP conjugated goat anti-rabbit IgG (ab6721; Abcam, Cambridge, United Kingdom). Rabbit plasma was diluted 1:500 to obtain absorbance values within the linear range of the assay. Each symbol represents the absorbance value of a single inoculated rabbit at 0, 2, 4, 8, or 12 weeks post-infection within each group. b Total rabbit IgG was quantified using the Abcam Rabbit IgG ELISA Kit in accordance with the provided protocol (ab187400; Abcam, Cambridge, United Kingdom). Plasma samples were diluted 1:1 × 10 6 . Each symbol represents total IgG of a single inoculated rabbit at 0, 2, or 12 weeks post-infection within each group. Bars represent mean absorbance or IgG values. Mixed model analyses with a Bonferroni correction were performed in weeks 8 and 12 (HTLV-1-specific) or 2 and 12 (total rabbit IgG) to determine statistical significance. A p

    Article Snippet: The supplied horseradish peroxidase (HRP) conjugated goat anti-human IgG was substituted for an HRP conjugated goat anti-rabbit IgG (ab6721; Abcam, Cambridge, United Kingdom).

    Techniques: Binding Assay, Modification, Infection, Enzyme-linked Immunosorbent Assay

    Immunoblot analysis to confirm the expression of EpCAM–IgM Fc in representative T1 transgenic lines. ( A ) T1 transgenic plants #2–9, 2–14, 2–17, 2–21, 2–23, and 2–27 were tested by goat anti-human IgM Fc µ-chain conjugated to HRP. M, protein marker; +, tobacco plant EpCAM–IgM Fc as a positive control; −, non-transgenic plant as a negative control. ( B ) T1 transgenic plants #2–9, 2–14, 2–17, 2–21, 2–23, and 2–27 were tested by mouse anti-human EpCAM as the first antibody and goat anti-mouse IgG 2a conjugated to HRP as the second antibody. M, protein marker; +, tobacco plant EpCAM–IgM Fc as a positive control (50 ng); −, non-transgenic plant as a negative control; hygromycin phosphotransferase (HTP).

    Journal: Plants

    Article Title: Expression of Colorectal Cancer Antigenic Protein Fused to IgM Fc in Chinese Cabbage (Brassica rapa)

    doi: 10.3390/plants9111466

    Figure Lengend Snippet: Immunoblot analysis to confirm the expression of EpCAM–IgM Fc in representative T1 transgenic lines. ( A ) T1 transgenic plants #2–9, 2–14, 2–17, 2–21, 2–23, and 2–27 were tested by goat anti-human IgM Fc µ-chain conjugated to HRP. M, protein marker; +, tobacco plant EpCAM–IgM Fc as a positive control; −, non-transgenic plant as a negative control. ( B ) T1 transgenic plants #2–9, 2–14, 2–17, 2–21, 2–23, and 2–27 were tested by mouse anti-human EpCAM as the first antibody and goat anti-mouse IgG 2a conjugated to HRP as the second antibody. M, protein marker; +, tobacco plant EpCAM–IgM Fc as a positive control (50 ng); −, non-transgenic plant as a negative control; hygromycin phosphotransferase (HTP).

    Article Snippet: Then, the plates were treated with rabbit anti-human IgG Fcγ antibody conjugated to HRP (Jackson Immunolab, West Grove, PA, USA) or goat anti-human IgM Fc µ-chain antibody conjugated to HRP (Abcam, Cambridge, UK) diluted 1:5000 in 1X PBS-T or 1X PBS and incubated for 2 h at RT.

    Techniques: Expressing, Transgenic Assay, Marker, Positive Control, Negative Control

    Enzyme-linked immunosorbent assay (ELISA) was performed to confirm binding interaction between anti-IgM Fc µ-chain antibody and EpCAM–IgM Fc × J-ChainK purified from F 1 transgenic Chinese cabbage plant. Each ELISA plate well was coated with 10 ng of the purified Chinse cabbage-derived EpCAM–IgM Fc × J-ChainK (EpCAM–IgM Fc C × J-ChainK C ), tobacco-derived EpCAM–IgM Fc × J-ChainK (EpCAM–IgM Fc T x J-ChainK T ), or tobacco-derived EpCAM–IgG Fc (EpCAM–IgG Fc T ). Each anti-IgM Fc µ-chain antibody and anti-IgG Fc antibody conjugated to HRP was applied to the coated well. Error bars indicate standard deviation (* p

    Journal: Plants

    Article Title: Expression of Colorectal Cancer Antigenic Protein Fused to IgM Fc in Chinese Cabbage (Brassica rapa)

    doi: 10.3390/plants9111466

    Figure Lengend Snippet: Enzyme-linked immunosorbent assay (ELISA) was performed to confirm binding interaction between anti-IgM Fc µ-chain antibody and EpCAM–IgM Fc × J-ChainK purified from F 1 transgenic Chinese cabbage plant. Each ELISA plate well was coated with 10 ng of the purified Chinse cabbage-derived EpCAM–IgM Fc × J-ChainK (EpCAM–IgM Fc C × J-ChainK C ), tobacco-derived EpCAM–IgM Fc × J-ChainK (EpCAM–IgM Fc T x J-ChainK T ), or tobacco-derived EpCAM–IgG Fc (EpCAM–IgG Fc T ). Each anti-IgM Fc µ-chain antibody and anti-IgG Fc antibody conjugated to HRP was applied to the coated well. Error bars indicate standard deviation (* p

    Article Snippet: Then, the plates were treated with rabbit anti-human IgG Fcγ antibody conjugated to HRP (Jackson Immunolab, West Grove, PA, USA) or goat anti-human IgM Fc µ-chain antibody conjugated to HRP (Abcam, Cambridge, UK) diluted 1:5000 in 1X PBS-T or 1X PBS and incubated for 2 h at RT.

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Purification, Transgenic Assay, Derivative Assay, Standard Deviation

    Immunoblot analysis to confirm the expression of EpCAM–IgM Fc in representative F 1 transgenic lines. ( A ) F 1 transgenic plants M209-1–209-10 were tested by goat anti-IgM Fc µ-chain conjugated to HRP. protein marker (M); +, tobacco plant EpCAM–IgM Fc as a positive control; − non-transgenic plant as a negative control. ( B ) F 1 transgenic plants M209-1–209-10 were tested by mouse anti-human EpCAM as the first antibody and goat anti-mouse IgG 2a conjugated to HRP as the second antibody. 2–9, T 1 transgenic plant #2–9 ( Figure 6 ); +, tobacco plant EpCAM–IgM Fc as a positive control (50 ng); −, non-transgenic plant (NT) as a negative control; hygromycin phosphotransferase (HTP).

    Journal: Plants

    Article Title: Expression of Colorectal Cancer Antigenic Protein Fused to IgM Fc in Chinese Cabbage (Brassica rapa)

    doi: 10.3390/plants9111466

    Figure Lengend Snippet: Immunoblot analysis to confirm the expression of EpCAM–IgM Fc in representative F 1 transgenic lines. ( A ) F 1 transgenic plants M209-1–209-10 were tested by goat anti-IgM Fc µ-chain conjugated to HRP. protein marker (M); +, tobacco plant EpCAM–IgM Fc as a positive control; − non-transgenic plant as a negative control. ( B ) F 1 transgenic plants M209-1–209-10 were tested by mouse anti-human EpCAM as the first antibody and goat anti-mouse IgG 2a conjugated to HRP as the second antibody. 2–9, T 1 transgenic plant #2–9 ( Figure 6 ); +, tobacco plant EpCAM–IgM Fc as a positive control (50 ng); −, non-transgenic plant (NT) as a negative control; hygromycin phosphotransferase (HTP).

    Article Snippet: Then, the plates were treated with rabbit anti-human IgG Fcγ antibody conjugated to HRP (Jackson Immunolab, West Grove, PA, USA) or goat anti-human IgM Fc µ-chain antibody conjugated to HRP (Abcam, Cambridge, UK) diluted 1:5000 in 1X PBS-T or 1X PBS and incubated for 2 h at RT.

    Techniques: Expressing, Transgenic Assay, Marker, Positive Control, Negative Control

    Immunoblot analysis to confirm the expression of EpCAM–IgM Fc in T 0 transgenic lines. ( A ) T 0 transgenic plants #2, 6, 7, 9, and 11 were tested by goat anti-IgM Fc µ-chain conjugated to HRP. M, protein marker; +, tobacco plant EpCAM–IgM Fc as a positive control; −, non-transgenic plant as a negative control. ( B ) T 0 transgenic plants #2, 6, 7, 9, and 11 were tested by mouse anti-human EpCAM as a primary first antibody and goat anti-mouse IgG 2a heavy chain conjugated to HRP as a secondary antibody. M, protein marker; +, tobacco plant EpCAM–IgM Fc as a positive control (50 ng); −, non-transgenic plant as a negative control; hygromycin phosphotransferase (HTP).

    Journal: Plants

    Article Title: Expression of Colorectal Cancer Antigenic Protein Fused to IgM Fc in Chinese Cabbage (Brassica rapa)

    doi: 10.3390/plants9111466

    Figure Lengend Snippet: Immunoblot analysis to confirm the expression of EpCAM–IgM Fc in T 0 transgenic lines. ( A ) T 0 transgenic plants #2, 6, 7, 9, and 11 were tested by goat anti-IgM Fc µ-chain conjugated to HRP. M, protein marker; +, tobacco plant EpCAM–IgM Fc as a positive control; −, non-transgenic plant as a negative control. ( B ) T 0 transgenic plants #2, 6, 7, 9, and 11 were tested by mouse anti-human EpCAM as a primary first antibody and goat anti-mouse IgG 2a heavy chain conjugated to HRP as a secondary antibody. M, protein marker; +, tobacco plant EpCAM–IgM Fc as a positive control (50 ng); −, non-transgenic plant as a negative control; hygromycin phosphotransferase (HTP).

    Article Snippet: Then, the plates were treated with rabbit anti-human IgG Fcγ antibody conjugated to HRP (Jackson Immunolab, West Grove, PA, USA) or goat anti-human IgM Fc µ-chain antibody conjugated to HRP (Abcam, Cambridge, UK) diluted 1:5000 in 1X PBS-T or 1X PBS and incubated for 2 h at RT.

    Techniques: Expressing, Transgenic Assay, Marker, Positive Control, Negative Control

    Binding of the identified compounds to the p21-binding domain (PBD). ( a ) Binding sensorgram for compound 2 . PBD or glutathione S -transferase (GST; control) was immobilized on the gold surface, and then various concentrations of compound 2 were passed over the immobilized PBD or GST (RU: response unit). ( b ) The K d values for the compound–PBD interactions as measured by SPR. ( c ) Effect of a mutation in the CRIB motif on the interaction of PBD with compound 2 . Compound 2 (100 μ M ) was passed over the immobilized wild-type PBD (Wt-PBD) or the PBD with two mutations (H83L/H86L; PBD-LL). All data are representative of at least three independent experiments.

    Journal: Experimental & Molecular Medicine

    Article Title: Small molecules that allosterically inhibit p21-activated kinase activity by binding to the regulatory p21-binding domain

    doi: 10.1038/emm.2016.13

    Figure Lengend Snippet: Binding of the identified compounds to the p21-binding domain (PBD). ( a ) Binding sensorgram for compound 2 . PBD or glutathione S -transferase (GST; control) was immobilized on the gold surface, and then various concentrations of compound 2 were passed over the immobilized PBD or GST (RU: response unit). ( b ) The K d values for the compound–PBD interactions as measured by SPR. ( c ) Effect of a mutation in the CRIB motif on the interaction of PBD with compound 2 . Compound 2 (100 μ M ) was passed over the immobilized wild-type PBD (Wt-PBD) or the PBD with two mutations (H83L/H86L; PBD-LL). All data are representative of at least three independent experiments.

    Article Snippet: Horseradish peroxidase (HRP)-conjugated anti-glutathione S -transferase (GST) antibodies were purchased from Abcam (Cambridge, MA, USA), anti-phospho-PAK antibodies from Cell Signaling Technology, Inc. (Danvers, MA, USA), anti-total myelin basic protein (MBP) antibodies from Millipore (Billerica, MA, USA) and anti-myc antibodies from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

    Techniques: Binding Assay, SPR Assay, Mutagenesis