horseradish peroxidase conjugated secondary antibody  (Abcam)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Abcam horseradish peroxidase conjugated secondary antibody
    Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibody/product/Abcam
    Average 99 stars, based on 96 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Gtr/Ego-independent TORC1 activation is achieved through a glutamine-sensitive interaction with Pib2 on the vacuolar membrane
    Article Snippet: .. Antibodies Anti-protein A (P-3775; Sigma-Aldrich), anti-GFP (11814460001; Roche), anti-Tor1 (sc-11900; Santa Cruz Biotechnology), anti-PGK (459250; Thermo Fisher Scientific), rabbit anti-Goat IgG HRP (anti-TAP) (ab6741; abcam), goat anti-mouse IgG, human ads-HRP (1030–05; Southern Bio Tech), anti-rabbit IgG HRP-linked antibody (7074S; Cell signaling), anti-Atg13 (a gift from Dr. Yoshinori Ohsumi, Tokyo Institute of Technology), and anti-HA (901501; BioLegend) antibodies were used for western blotting. .. Immunoprecipitation experiments Cells were resuspended in TAP-A buffer (50 mM Tris-HCl (pH 8.0) (Wako), 150 mM NaCl (Wako), 10% glycerol (Wako), 1 mM DTT (Wako), 1 mM EDTA (Sigma) supplemented with Complete EDTA-free protease inhibitor cocktail (Roche), 1 mM PMSF (Wako), 1 μM microcystin-LR (Wako) and PhosSTOP (Roche), and lysed using a FastPrep instrument (MP-Biomedicals) and zirconia beads.

    Article Title: Apolipoprotein A‐I Modulates Atherosclerosis Through Lymphatic Vessel‐Dependent Mechanisms in Mice
    Article Snippet: .. Antibodies against CLEC‐2 (R & D Systems, AF1718) and pAkt (Cell Signalling, 9275) were incubated with the membranes overnight at 4°C, and a horseradish peroxidase–conjugated secondary antibody (Abcam, AB6741) was used for detection using the Western Lightning Ultra chemiluminescence kit (PerkinElmer). .. Organ Harvesting LNs, ears, dermal back skin sections, aortas, hearts, and popliteal collecting lymphatic vessels were harvested and either freshly processed for flow cytometry analysis and/or Western blots, or fixed in 4% paraformaldehyde and 10% formalin for future analysis.

    Article Title: Gtr/Ego-independent TORC1 activation is achieved through a glutamine-sensitive interaction with Pib2 on the vacuolar membrane
    Article Snippet: .. Anti-protein A (P-3775; Sigma-Aldrich), anti-GFP (11814460001; Roche), anti-Tor1 (sc-11900; Santa Cruz Biotechnology), anti-PGK (459250; Thermo Fisher Scientific), rabbit anti-Goat IgG HRP (anti-TAP) (ab6741; abcam), goat anti-mouse IgG, human ads-HRP (1030–05; Southern Bio Tech), anti-rabbit IgG HRP-linked antibody (7074S; Cell signaling), anti-Atg13 (a gift from Dr. Yoshinori Ohsumi, Tokyo Institute of Technology), and anti-HA (901501; BioLegend) antibodies were used for western blotting. .. Cells were resuspended in TAP-A buffer (50 mM Tris-HCl (pH 8.0) (Wako), 150 mM NaCl (Wako), 10% glycerol (Wako), 1 mM DTT (Wako), 1 mM EDTA (Sigma) supplemented with Complete EDTA-free protease inhibitor cocktail (Roche), 1 mM PMSF (Wako), 1 μM microcystin-LR (Wako) and PhosSTOP (Roche), and lysed using a FastPrep instrument (MP-Biomedicals) and zirconia beads.

    Incubation:

    Article Title: TFF3 and TFF1 expression levels are elevated in colorectal cancer and promote the malignant behavior of colon cancer by activating the EMT process
    Article Snippet: .. Following a TBS-Tween wash, the membranes were incubated with goat anti-rabbit secondary antibody (HRP; 1:10,000, ab6721) or rabbit anti-goat secondary antibody (HRP; 1:5,000, ab6741) (both from Abcam) in blocking solution for 1 h at room temperature. .. Immunoreactive proteins were detected using enhanced chemiluminescence kit (Thermo Scientific Pierce ECL Substrate).

    Article Title: Apolipoprotein A‐I Modulates Atherosclerosis Through Lymphatic Vessel‐Dependent Mechanisms in Mice
    Article Snippet: .. Antibodies against CLEC‐2 (R & D Systems, AF1718) and pAkt (Cell Signalling, 9275) were incubated with the membranes overnight at 4°C, and a horseradish peroxidase–conjugated secondary antibody (Abcam, AB6741) was used for detection using the Western Lightning Ultra chemiluminescence kit (PerkinElmer). .. Organ Harvesting LNs, ears, dermal back skin sections, aortas, hearts, and popliteal collecting lymphatic vessels were harvested and either freshly processed for flow cytometry analysis and/or Western blots, or fixed in 4% paraformaldehyde and 10% formalin for future analysis.

    Article Title: Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF-α
    Article Snippet: .. Following washing, membranes were incubated with the following corresponding secondary antibodies at 37°C for 60 min: Horseradish peroxidase (HRP)-tagged goat anti-mouse IgG H & L (cat. no. ab6789; 1:7,000; Abcam), HRP-tagged rabbit anti-mouse IgG H & L (cat. no. ab6728; 1:8,000; Abcam) and HRP-tagged rabbit anti-goat IgG H & L (cat. no. ab97100; 1:8,000; Abcam). .. Finally, the blots were visualized by enhanced chemiluminescent reagent (EMD Millipore, Billerica, MA, USA).

    other:

    Article Title: An Antibody-Immobilized Silica Inverse Opal Nanostructure for Label-Free Optical Biosensors
    Article Snippet: Horseradish Peroxidase (HRP) conjugated anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H & L (HRP)) was purchased from Abcam Co. (Cambridge, UK).

    Blocking Assay:

    Article Title: TFF3 and TFF1 expression levels are elevated in colorectal cancer and promote the malignant behavior of colon cancer by activating the EMT process
    Article Snippet: .. Following a TBS-Tween wash, the membranes were incubated with goat anti-rabbit secondary antibody (HRP; 1:10,000, ab6721) or rabbit anti-goat secondary antibody (HRP; 1:5,000, ab6741) (both from Abcam) in blocking solution for 1 h at room temperature. .. Immunoreactive proteins were detected using enhanced chemiluminescence kit (Thermo Scientific Pierce ECL Substrate).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Abcam peroxidase conjugated anti mouse secondary antibody
    Effects of resveratrol on NIS protein expression in rat thyroid glands. Male Sprague-Dawley rats were treated with the control vehicle (Control, n = 4) or with 50 mg/kg/day resveratrol i.p. (Resv, n = 4), for 14 days. On day 15 th , the animals were sacrificed and their thyroids were removed. Immunofluorescence analysis was performed using a <t>mouse</t> monoclonal <t>anti-NIS</t> antibody and an anti-mouse <t>fluorescein-conjugated</t> <t>secondary</t> antibody, Alexa Fluor 488, (green). Po-Pro-3 iodide was used to stain the nuclei (red). The negative control was performed using a mouse IgG preparations instead of the primary antibody (data not shown). The slides were visualized under a Zeiss LSM S10 confocal microscope with a x40 immersion lens. Representative data from four experiments are showed.
    Peroxidase Conjugated Anti Mouse Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated anti mouse secondary antibody/product/Abcam
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated anti mouse secondary antibody - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    93
    Abcam hrp conjugated goat anti rabbit igg
    Binding ELISA demonstrating the autoantibody binding to Met5A mesothelial cells due to the presence of MCAA in serum from human patients exposed to LA fibers. Sera were pooled from MCAA positive samples (MCAA+) and MCAA-positive samples cleared of all <t>IgG</t> (Cleared). Human mesothelial cells were plated in 96-well plates and then stained for MCAA binding using the pooled serum as the primary antibody and anti-human IgG - <t>HRP</t> for the secondary antibody. N= 3 samples per treatment group. Data are mean absorbance at 450 nm. *=p
    Hrp Conjugated Goat Anti Rabbit Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti rabbit igg/product/Abcam
    Average 93 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti rabbit igg - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Abcam horse radish peroxidase hrp conjugated rabbit anti mouse secondary antibody
    Evaluation of the binding selectivity of the selected phage clones by cell ELISA Cells were seeded onto 96-well cell culture plates overnight (1×10 4 cells/well). 10 9 phage particles were added to each well. Phage binding to cells was detected through adding mouse <t>anti-M13</t> phage antibody and <t>HRP-conjugated</t> rabbit anti-mouse secondary antibody. OD was obtained after blocking the reaction. The selectivity values for phage binding was calculated by the formula mentioned in the text and were 5.1, 2.2, 2.9, 2.5, 1.4, and 1.3 for P1, P2, P3, P4, P5, and P6, respectively. P1 is indicated to be the strongest binder and binds more effectively than all phage clones to A549 cells
    Horse Radish Peroxidase Hrp Conjugated Rabbit Anti Mouse Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horse radish peroxidase hrp conjugated rabbit anti mouse secondary antibody/product/Abcam
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    horse radish peroxidase hrp conjugated rabbit anti mouse secondary antibody - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Effects of resveratrol on NIS protein expression in rat thyroid glands. Male Sprague-Dawley rats were treated with the control vehicle (Control, n = 4) or with 50 mg/kg/day resveratrol i.p. (Resv, n = 4), for 14 days. On day 15 th , the animals were sacrificed and their thyroids were removed. Immunofluorescence analysis was performed using a mouse monoclonal anti-NIS antibody and an anti-mouse fluorescein-conjugated secondary antibody, Alexa Fluor 488, (green). Po-Pro-3 iodide was used to stain the nuclei (red). The negative control was performed using a mouse IgG preparations instead of the primary antibody (data not shown). The slides were visualized under a Zeiss LSM S10 confocal microscope with a x40 immersion lens. Representative data from four experiments are showed.

    Journal: PLoS ONE

    Article Title: Resveratrol Inhibits Sodium/Iodide Symporter Gene Expression and Function in Rat Thyroid Cells

    doi: 10.1371/journal.pone.0107936

    Figure Lengend Snippet: Effects of resveratrol on NIS protein expression in rat thyroid glands. Male Sprague-Dawley rats were treated with the control vehicle (Control, n = 4) or with 50 mg/kg/day resveratrol i.p. (Resv, n = 4), for 14 days. On day 15 th , the animals were sacrificed and their thyroids were removed. Immunofluorescence analysis was performed using a mouse monoclonal anti-NIS antibody and an anti-mouse fluorescein-conjugated secondary antibody, Alexa Fluor 488, (green). Po-Pro-3 iodide was used to stain the nuclei (red). The negative control was performed using a mouse IgG preparations instead of the primary antibody (data not shown). The slides were visualized under a Zeiss LSM S10 confocal microscope with a x40 immersion lens. Representative data from four experiments are showed.

    Article Snippet: The membranes were subsequently washed and incubated with a horseradish peroxidase-conjugated anti-mouse secondary antibody (ab6789, Abcam, Cambridge, UK), following the manufacturer instructions.

    Techniques: Expressing, Immunofluorescence, Staining, Negative Control, Microscopy

    Conjugation efficiency and biodistribution analysis of CRISPR-modified antibody. ( a ) Coomassie blue stained gel of sortase-mediated conjugation of modified antibody to GGG-fluorophore. ( b ) Fluorescence scan of the antibody-fluorophore, stained with anti-Flag-HRP, showing the antibody conjugated fluorophore (red tab) compared to free fluorophore (black tab). ( c ) HPLC fluorescence trace antibody-fluorophore conjugates at different molar ratios. ( d ) Quantitative analysis of the percent of antibody modification. ( e ) Binding of 125 I-labeled CRISPR-modified antibody to REN-ICAM and REN-WT cells. ( f ) Biodistribution of 125 I-labeled CRISPR-modified anti-ICAM mAb in mice at 30 min. Tissue uptake is indicated as mean ± SEM (n = 3). Biodistribution analysis was performed comparing 111 In-labeled site-specific CRISPR/Cas9- to chemically-modified mAb. ( g ) Biodistribution of 111 In-labeled anti-ICAM mAb modified in mice at 30 min. Tissue uptake is indicated as mean ± SEM. ( h ) Localization ratio of selected organs. Significant differences were determined by t-test with Bonferroni correction to account for multiple comparisons.

    Journal: Scientific Reports

    Article Title: Molecular engineering of antibodies for site-specific covalent conjugation using CRISPR/Cas9

    doi: 10.1038/s41598-018-19784-2

    Figure Lengend Snippet: Conjugation efficiency and biodistribution analysis of CRISPR-modified antibody. ( a ) Coomassie blue stained gel of sortase-mediated conjugation of modified antibody to GGG-fluorophore. ( b ) Fluorescence scan of the antibody-fluorophore, stained with anti-Flag-HRP, showing the antibody conjugated fluorophore (red tab) compared to free fluorophore (black tab). ( c ) HPLC fluorescence trace antibody-fluorophore conjugates at different molar ratios. ( d ) Quantitative analysis of the percent of antibody modification. ( e ) Binding of 125 I-labeled CRISPR-modified antibody to REN-ICAM and REN-WT cells. ( f ) Biodistribution of 125 I-labeled CRISPR-modified anti-ICAM mAb in mice at 30 min. Tissue uptake is indicated as mean ± SEM (n = 3). Biodistribution analysis was performed comparing 111 In-labeled site-specific CRISPR/Cas9- to chemically-modified mAb. ( g ) Biodistribution of 111 In-labeled anti-ICAM mAb modified in mice at 30 min. Tissue uptake is indicated as mean ± SEM. ( h ) Localization ratio of selected organs. Significant differences were determined by t-test with Bonferroni correction to account for multiple comparisons.

    Article Snippet: The membrane was incubated with anti-FLAG M2 antibody (F3165; Sigma-Aldrich, St. Louis, MO), followed by goat anti-mouse HRP secondary antibody (ab6789; Abcam, Cambridge, MA).

    Techniques: Conjugation Assay, CRISPR, Modification, Staining, Fluorescence, High Performance Liquid Chromatography, Binding Assay, Labeling, Mouse Assay

    Schematic diagram and characterization of CRISPR/Cas9 genome editing of hybridoma cells for site-specific modification of antibodies. C-terminal end of Ig gamma-2B gene from ( a ) rat chromosome 13q24, ( b ) Fcgr2b locus, was modified by ( c ) homology directed repair to incorporate sortase and flag tags. sgRNAs with best On-Target/Off-Target scores were selected in the region near the IgG2b stop codon. ( d ) Anti-ICAMIgG2b antibodies were generated incorporating Sortase and FLAG tags at their C-terminal end. Confirmation of insert was performed by ( e ) Agarose gel analysis of PCR fragments from positive and negative clones. ( f ) Verification of integration in positive clones by sanger sequencing. ( g ) Coomassie blue stained gel of three positive clones. ( h ) Western blot of a positive clone under reducing and non-reducing conditions, stained with anti-Flag-HRP.

    Journal: Scientific Reports

    Article Title: Molecular engineering of antibodies for site-specific covalent conjugation using CRISPR/Cas9

    doi: 10.1038/s41598-018-19784-2

    Figure Lengend Snippet: Schematic diagram and characterization of CRISPR/Cas9 genome editing of hybridoma cells for site-specific modification of antibodies. C-terminal end of Ig gamma-2B gene from ( a ) rat chromosome 13q24, ( b ) Fcgr2b locus, was modified by ( c ) homology directed repair to incorporate sortase and flag tags. sgRNAs with best On-Target/Off-Target scores were selected in the region near the IgG2b stop codon. ( d ) Anti-ICAMIgG2b antibodies were generated incorporating Sortase and FLAG tags at their C-terminal end. Confirmation of insert was performed by ( e ) Agarose gel analysis of PCR fragments from positive and negative clones. ( f ) Verification of integration in positive clones by sanger sequencing. ( g ) Coomassie blue stained gel of three positive clones. ( h ) Western blot of a positive clone under reducing and non-reducing conditions, stained with anti-Flag-HRP.

    Article Snippet: The membrane was incubated with anti-FLAG M2 antibody (F3165; Sigma-Aldrich, St. Louis, MO), followed by goat anti-mouse HRP secondary antibody (ab6789; Abcam, Cambridge, MA).

    Techniques: CRISPR, Modification, Generated, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Clone Assay, Sequencing, Staining, Western Blot

    Binding ELISA demonstrating the autoantibody binding to Met5A mesothelial cells due to the presence of MCAA in serum from human patients exposed to LA fibers. Sera were pooled from MCAA positive samples (MCAA+) and MCAA-positive samples cleared of all IgG (Cleared). Human mesothelial cells were plated in 96-well plates and then stained for MCAA binding using the pooled serum as the primary antibody and anti-human IgG - HRP for the secondary antibody. N= 3 samples per treatment group. Data are mean absorbance at 450 nm. *=p

    Journal: Inhalation toxicology

    Article Title: Mesothelial Cell Autoantibodies Upregulate Transcription Factors Associated with Fibrosis

    doi: 10.1080/08958378.2016.1271841

    Figure Lengend Snippet: Binding ELISA demonstrating the autoantibody binding to Met5A mesothelial cells due to the presence of MCAA in serum from human patients exposed to LA fibers. Sera were pooled from MCAA positive samples (MCAA+) and MCAA-positive samples cleared of all IgG (Cleared). Human mesothelial cells were plated in 96-well plates and then stained for MCAA binding using the pooled serum as the primary antibody and anti-human IgG - HRP for the secondary antibody. N= 3 samples per treatment group. Data are mean absorbance at 450 nm. *=p

    Article Snippet: The secondary antibody used was HRP-conjugated goat anti-rabbit IgG (ABCAM) diluted 1:1000 with 3%BSA/PBS.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Staining

    Evaluation of the binding selectivity of the selected phage clones by cell ELISA Cells were seeded onto 96-well cell culture plates overnight (1×10 4 cells/well). 10 9 phage particles were added to each well. Phage binding to cells was detected through adding mouse anti-M13 phage antibody and HRP-conjugated rabbit anti-mouse secondary antibody. OD was obtained after blocking the reaction. The selectivity values for phage binding was calculated by the formula mentioned in the text and were 5.1, 2.2, 2.9, 2.5, 1.4, and 1.3 for P1, P2, P3, P4, P5, and P6, respectively. P1 is indicated to be the strongest binder and binds more effectively than all phage clones to A549 cells

    Journal: Iranian Journal of Pharmaceutical Research : IJPR

    Article Title: Identification of a Novel Tumor-Binding Peptide for Lung Cancer Through in-vitro Panning

    doi:

    Figure Lengend Snippet: Evaluation of the binding selectivity of the selected phage clones by cell ELISA Cells were seeded onto 96-well cell culture plates overnight (1×10 4 cells/well). 10 9 phage particles were added to each well. Phage binding to cells was detected through adding mouse anti-M13 phage antibody and HRP-conjugated rabbit anti-mouse secondary antibody. OD was obtained after blocking the reaction. The selectivity values for phage binding was calculated by the formula mentioned in the text and were 5.1, 2.2, 2.9, 2.5, 1.4, and 1.3 for P1, P2, P3, P4, P5, and P6, respectively. P1 is indicated to be the strongest binder and binds more effectively than all phage clones to A549 cells

    Article Snippet: Mouse anti-M13 phage antibody and horse radish peroxidase (HRP)-conjugated rabbit anti-mouse secondary antibody were purchased from Abcam Inc (MA, USA).

    Techniques: Binding Assay, Clone Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Blocking Assay