goat anti rabbit igg horseradish peroxidase conjugated secondary antibody  (Abcam)

 
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    Structured Review

    Abcam goat anti rabbit igg horseradish peroxidase conjugated secondary antibody
    Goat Anti Rabbit Igg Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg horseradish peroxidase conjugated secondary antibody/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit igg horseradish peroxidase conjugated secondary antibody - by Bioz Stars, 2022-05
    99/100 stars

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    Abcam goat anti rabbit igg h l hrp
    Ablation of HTLV-1 CTCF-binding site significantly decreases HTLV-1-specific antibody response, but not total rabbit <t>IgG.</t> a Antibody response was quantified using a modified Avioq HTLV-1/2 Microelisa System protocol (Avioq, Inc., Research Triangle Park, NC). The supplied horseradish peroxidase <t>(HRP)</t> conjugated goat anti-human immunoglobulin (Ig) was substituted for an HRP conjugated goat anti-rabbit IgG (ab6721; Abcam, Cambridge, United Kingdom). Rabbit plasma was diluted 1:500 to obtain absorbance values within the linear range of the assay. Each symbol represents the absorbance value of a single inoculated rabbit at 0, 2, 4, 8, or 12 weeks post-infection within each group. b Total rabbit IgG was quantified using the Abcam Rabbit IgG ELISA Kit in accordance with the provided protocol (ab187400; Abcam, Cambridge, United Kingdom). Plasma samples were diluted 1:1 × 10 6 . Each symbol represents total IgG of a single inoculated rabbit at 0, 2, or 12 weeks post-infection within each group. Bars represent mean absorbance or IgG values. Mixed model analyses with a Bonferroni correction were performed in weeks 8 and 12 (HTLV-1-specific) or 2 and 12 (total rabbit IgG) to determine statistical significance. A p
    Goat Anti Rabbit Igg H L Hrp, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg h l hrp/product/Abcam
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit igg h l hrp - by Bioz Stars, 2022-05
    97/100 stars
      Buy from Supplier

    99
    Abcam hrp conjugated goat anti rabbit igg
    Constructs features ( A ), levels of TCRαβ-chain expression in CD8 + T cells transduced with the three different constructs ( B ), polymerization ability of TCRαβ and CD3 chains encoded in the three constructs and host-encoded CD3 chains ( C ), and identity of the polymerized CD3 chains ( D ). Depicted in (A) are the three constructs. The boxes represent the amino acid sequences of the chains indicated inside the boxes. T2A: self-cleavage 2A region of the Thosea asigna virus; P2A: self-cleavage 2A region of the porcine Teschovirus 1; CD3: in construct 3 represent the extracellular and transmembrane domains of the CD3ζ chain, followed by the signaling domains of CD28, 4-1BB, and CD3ζ chains. The lines represent amino acid sequences of linkers: RAKR is the Furin consensus amino acid recognition site; SGSG is a hydrophilic tetrapeptide added to prevent steric hindrance. The arrows represent cleavage sites. In (B), FACS analysis results; transduced CD8 + T cells were stained with FITC–anti-CD8 Ab and PE-HLA-A*02:01/CT37 YLCSGSSYFV peptide complexes. A representative of three experiments is shown. Results using FITC-isotype Ab and PE-control pentamer complexes were negative (data not shown). In (C), biotinylated membrane proteins from CD8 + T cells were immunoprecipitated with an anti-TCRβ Ab and molecular complexes separated by SDS-PAGE, transferred to a membrane, and blots detected by chemiluminescence. A representative of three experiments is shown. In (D), immunoprecipitated proteins obtained as indicated in (C) for construct 3, were blotted using rabbit anti-CD3δ EP4426, anti-CD3γ 134684, anti-CD3ε 133628 Abs, and Armenian hamster anti-CD3ζ H136-968 Abs (Abcam), <t>HRP-conjugated</t> goat anti-rabbit <t>IgG</t> or HPR-conjugated rabbit anti-Armenian hamster IgG, and blots were detected by chemiluminescence using autoradiography films (Kodak BioMax Light). A representative of two experiments is shown.
    Hrp Conjugated Goat Anti Rabbit Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti rabbit igg/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti rabbit igg - by Bioz Stars, 2022-05
    99/100 stars
      Buy from Supplier

    97
    Abcam horseradish peroxidase conjugated rabbit anti mouse igg
    Effect of MTOM-adjuvanted WH121 protein on serum antibody levels in immunized mice . Six weeks after final immunization, the levels of serum <t>IgG,</t> <t>IgG1,</t> and IgG2c (replaced with IgG2a) antibodies from immunized mice were detected using ELISA. (A) WH121/MTOM induced IgG, IgG1, and IgG2a antibodies specific to WH121. (B) Comparison of antibody levels induced by WH121/MTO, WH121/Mv, and WH121/MTOM. Results are shown as mean (±SEM) log 10 end point titers and the ratio of IgG2a:IgG1 in the differently vaccinated groups ( n = 3).
    Horseradish Peroxidase Conjugated Rabbit Anti Mouse Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated rabbit anti mouse igg/product/Abcam
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated rabbit anti mouse igg - by Bioz Stars, 2022-05
    97/100 stars
      Buy from Supplier

    Image Search Results


    Ablation of HTLV-1 CTCF-binding site significantly decreases HTLV-1-specific antibody response, but not total rabbit IgG. a Antibody response was quantified using a modified Avioq HTLV-1/2 Microelisa System protocol (Avioq, Inc., Research Triangle Park, NC). The supplied horseradish peroxidase (HRP) conjugated goat anti-human immunoglobulin (Ig) was substituted for an HRP conjugated goat anti-rabbit IgG (ab6721; Abcam, Cambridge, United Kingdom). Rabbit plasma was diluted 1:500 to obtain absorbance values within the linear range of the assay. Each symbol represents the absorbance value of a single inoculated rabbit at 0, 2, 4, 8, or 12 weeks post-infection within each group. b Total rabbit IgG was quantified using the Abcam Rabbit IgG ELISA Kit in accordance with the provided protocol (ab187400; Abcam, Cambridge, United Kingdom). Plasma samples were diluted 1:1 × 10 6 . Each symbol represents total IgG of a single inoculated rabbit at 0, 2, or 12 weeks post-infection within each group. Bars represent mean absorbance or IgG values. Mixed model analyses with a Bonferroni correction were performed in weeks 8 and 12 (HTLV-1-specific) or 2 and 12 (total rabbit IgG) to determine statistical significance. A p

    Journal: Retrovirology

    Article Title: HTLV-1 CTCF-binding site is dispensable for in vitro immortalization and persistent infection in vivo

    doi: 10.1186/s12977-019-0507-9

    Figure Lengend Snippet: Ablation of HTLV-1 CTCF-binding site significantly decreases HTLV-1-specific antibody response, but not total rabbit IgG. a Antibody response was quantified using a modified Avioq HTLV-1/2 Microelisa System protocol (Avioq, Inc., Research Triangle Park, NC). The supplied horseradish peroxidase (HRP) conjugated goat anti-human immunoglobulin (Ig) was substituted for an HRP conjugated goat anti-rabbit IgG (ab6721; Abcam, Cambridge, United Kingdom). Rabbit plasma was diluted 1:500 to obtain absorbance values within the linear range of the assay. Each symbol represents the absorbance value of a single inoculated rabbit at 0, 2, 4, 8, or 12 weeks post-infection within each group. b Total rabbit IgG was quantified using the Abcam Rabbit IgG ELISA Kit in accordance with the provided protocol (ab187400; Abcam, Cambridge, United Kingdom). Plasma samples were diluted 1:1 × 10 6 . Each symbol represents total IgG of a single inoculated rabbit at 0, 2, or 12 weeks post-infection within each group. Bars represent mean absorbance or IgG values. Mixed model analyses with a Bonferroni correction were performed in weeks 8 and 12 (HTLV-1-specific) or 2 and 12 (total rabbit IgG) to determine statistical significance. A p

    Article Snippet: The supplied horseradish peroxidase (HRP) conjugated goat anti-human IgG was substituted for an HRP conjugated goat anti-rabbit IgG (ab6721; Abcam, Cambridge, United Kingdom).

    Techniques: Binding Assay, Modification, Infection, Enzyme-linked Immunosorbent Assay

    Binding of the selected representatives of ARS sequence families to the immobilized human IL-17RA-IgG chimera in ELISA. Purified binding proteins were produced in the form of in vivo biotinylated His 6 -ARS-TolA-AVI fusion proteins. Binding to IL-17RA-IgG was visualized by streptavidin-HRP conjugate. Each point represents the mean value ± standard deviation (SD).

    Journal: International Journal of Molecular Sciences

    Article Title: ABD-Derived Protein Blockers of Human IL-17 Receptor A as Non-IgG Alternatives for Modulation of IL-17-Dependent Pro-Inflammatory Axis

    doi: 10.3390/ijms19103089

    Figure Lengend Snippet: Binding of the selected representatives of ARS sequence families to the immobilized human IL-17RA-IgG chimera in ELISA. Purified binding proteins were produced in the form of in vivo biotinylated His 6 -ARS-TolA-AVI fusion proteins. Binding to IL-17RA-IgG was visualized by streptavidin-HRP conjugate. Each point represents the mean value ± standard deviation (SD).

    Article Snippet: Goat anti-rabbit IgG-HRP conjugate was obtained from Abcam plc., Cambridge, UK.

    Techniques: Binding Assay, Sequencing, Enzyme-linked Immunosorbent Assay, Purification, Produced, In Vivo, Standard Deviation

    ARS ligands compete with IL-17A cytokine for binding to the human IL-17RA-IgG receptor chimera. The IL-17RA-IgG chimera was immobilized on an ELISA plate and serially diluted inhibitory His 6 -ARS-TolA-AVI ligands were used to compete for binding with 10 nM IL-17A. Bound IL-17A was detected with anti-IL-17A polyclonal antibody in combination with secondary anti-IgG-HRP conjugate. His 6 -ABDwt-TolA-AVI served as a negative control. Error bars represent standard deviations (SDs).

    Journal: International Journal of Molecular Sciences

    Article Title: ABD-Derived Protein Blockers of Human IL-17 Receptor A as Non-IgG Alternatives for Modulation of IL-17-Dependent Pro-Inflammatory Axis

    doi: 10.3390/ijms19103089

    Figure Lengend Snippet: ARS ligands compete with IL-17A cytokine for binding to the human IL-17RA-IgG receptor chimera. The IL-17RA-IgG chimera was immobilized on an ELISA plate and serially diluted inhibitory His 6 -ARS-TolA-AVI ligands were used to compete for binding with 10 nM IL-17A. Bound IL-17A was detected with anti-IL-17A polyclonal antibody in combination with secondary anti-IgG-HRP conjugate. His 6 -ABDwt-TolA-AVI served as a negative control. Error bars represent standard deviations (SDs).

    Article Snippet: Goat anti-rabbit IgG-HRP conjugate was obtained from Abcam plc., Cambridge, UK.

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Negative Control

    Constructs features ( A ), levels of TCRαβ-chain expression in CD8 + T cells transduced with the three different constructs ( B ), polymerization ability of TCRαβ and CD3 chains encoded in the three constructs and host-encoded CD3 chains ( C ), and identity of the polymerized CD3 chains ( D ). Depicted in (A) are the three constructs. The boxes represent the amino acid sequences of the chains indicated inside the boxes. T2A: self-cleavage 2A region of the Thosea asigna virus; P2A: self-cleavage 2A region of the porcine Teschovirus 1; CD3: in construct 3 represent the extracellular and transmembrane domains of the CD3ζ chain, followed by the signaling domains of CD28, 4-1BB, and CD3ζ chains. The lines represent amino acid sequences of linkers: RAKR is the Furin consensus amino acid recognition site; SGSG is a hydrophilic tetrapeptide added to prevent steric hindrance. The arrows represent cleavage sites. In (B), FACS analysis results; transduced CD8 + T cells were stained with FITC–anti-CD8 Ab and PE-HLA-A*02:01/CT37 YLCSGSSYFV peptide complexes. A representative of three experiments is shown. Results using FITC-isotype Ab and PE-control pentamer complexes were negative (data not shown). In (C), biotinylated membrane proteins from CD8 + T cells were immunoprecipitated with an anti-TCRβ Ab and molecular complexes separated by SDS-PAGE, transferred to a membrane, and blots detected by chemiluminescence. A representative of three experiments is shown. In (D), immunoprecipitated proteins obtained as indicated in (C) for construct 3, were blotted using rabbit anti-CD3δ EP4426, anti-CD3γ 134684, anti-CD3ε 133628 Abs, and Armenian hamster anti-CD3ζ H136-968 Abs (Abcam), HRP-conjugated goat anti-rabbit IgG or HPR-conjugated rabbit anti-Armenian hamster IgG, and blots were detected by chemiluminescence using autoradiography films (Kodak BioMax Light). A representative of two experiments is shown.

    Journal: The Journal of Immunology Author Choice

    Article Title: An Isolated TCR αβ Restricted by HLA-A*02:01/CT37 Peptide Redirecting CD8+ T Cells To Kill and Secrete IFN-γ in Response to Lung Adenocarcinoma Cell Lines

    doi: 10.4049/jimmunol.1701054

    Figure Lengend Snippet: Constructs features ( A ), levels of TCRαβ-chain expression in CD8 + T cells transduced with the three different constructs ( B ), polymerization ability of TCRαβ and CD3 chains encoded in the three constructs and host-encoded CD3 chains ( C ), and identity of the polymerized CD3 chains ( D ). Depicted in (A) are the three constructs. The boxes represent the amino acid sequences of the chains indicated inside the boxes. T2A: self-cleavage 2A region of the Thosea asigna virus; P2A: self-cleavage 2A region of the porcine Teschovirus 1; CD3: in construct 3 represent the extracellular and transmembrane domains of the CD3ζ chain, followed by the signaling domains of CD28, 4-1BB, and CD3ζ chains. The lines represent amino acid sequences of linkers: RAKR is the Furin consensus amino acid recognition site; SGSG is a hydrophilic tetrapeptide added to prevent steric hindrance. The arrows represent cleavage sites. In (B), FACS analysis results; transduced CD8 + T cells were stained with FITC–anti-CD8 Ab and PE-HLA-A*02:01/CT37 YLCSGSSYFV peptide complexes. A representative of three experiments is shown. Results using FITC-isotype Ab and PE-control pentamer complexes were negative (data not shown). In (C), biotinylated membrane proteins from CD8 + T cells were immunoprecipitated with an anti-TCRβ Ab and molecular complexes separated by SDS-PAGE, transferred to a membrane, and blots detected by chemiluminescence. A representative of three experiments is shown. In (D), immunoprecipitated proteins obtained as indicated in (C) for construct 3, were blotted using rabbit anti-CD3δ EP4426, anti-CD3γ 134684, anti-CD3ε 133628 Abs, and Armenian hamster anti-CD3ζ H136-968 Abs (Abcam), HRP-conjugated goat anti-rabbit IgG or HPR-conjugated rabbit anti-Armenian hamster IgG, and blots were detected by chemiluminescence using autoradiography films (Kodak BioMax Light). A representative of two experiments is shown.

    Article Snippet: To determine the identity of CD3 chains, immunoprecipitated proteins obtained as indicated above from cells transfected with construct 3 were blotted using rabbit anti-CD3δ EP4426, anti-CD3γ 134684, anti-CD3ε 133628, and Armenian hamster anti-CD3ζ H136-968 Abs (Abcam), 1:2000 HRP-conjugated goat anti-rabbit IgG or 1:000 HPR-conjugated rabbit anti-Armenian hamster IgG (Abcam), and the Immun-Star HRP kit, and blots were revealed by chemiluminescence using autoradiography Kodak BioMax Light film.

    Techniques: Construct, Expressing, Transduction, FACS, Staining, Immunoprecipitation, SDS Page, Autoradiography

    Features of lung ADC cell lines used ( A ) and cytotoxic capacity of CD8 + T cells transduced with the three different constructs ( B ). In (A), total RNA was isolated from cell lines and negative control (A549 cell line) as indicated, and RT-PCR results obtained. The mean of triplicates is shown (A i ). Protein lysates from cell lines indicted and negative control A549 were immunoprecipitated with rabbit polyclonal anti-human CT37 Ab HPA011284, proteins were separated by SDS-PAGE, transferred to a membrane, and CT37 blotted using rabbit polyclonal AP51690PU-N Ab and HRP-conjugated goat anti-rabbit IgG. Blots were detected by chemiluminescence using autoradiography films [Kodak BioMax Light; (Aii)]. In (B), retroviral transduction of effector (E) allogeneic CD8 + . For results presented in (Bi)–(Biii), six [ 51 Cr] release assays, with each variable in triplicate, were performed. After 2 cycles of 10 d stimulation in vitro, E cells were harvested, washed, counted, and plated to be tested at 16:1 E:T ratios. Target (T) lung ADC lines HLA-A*A-02:01– positive HCC2935 (Bi), and H1993 (Bii), and HLA-A*02:01– negative line H1299, were labeled with 200 μCi of Na 2 51 CrO 4 for 1 h and washed. [ 51 Cr]-released cytotoxic assay proceed for 4 h at a 16:1 E:T ratio. Cultures proceeded without or with an anti–HLA-A2 Ab to block the HLA-A*02:01 molecule, and at the indicated concentrations. The HCC2935 cell line carries wild type (wt) TP53 and KRAS genes, and an EGFR 2237_2254del10 (deletion E746-S752). The H1993 line carries a TP53 726C > G (C242W) substitution, and wt EGFR and KRAS genes. The H1299 carries a TP53 gene deletion, and wt EGFR and KRAS genes. Data on TP53 . We genotyped mutations in the EGFR and KRAS . The p values from Mann–Whitney comparison of two means are presented.

    Journal: The Journal of Immunology Author Choice

    Article Title: An Isolated TCR αβ Restricted by HLA-A*02:01/CT37 Peptide Redirecting CD8+ T Cells To Kill and Secrete IFN-γ in Response to Lung Adenocarcinoma Cell Lines

    doi: 10.4049/jimmunol.1701054

    Figure Lengend Snippet: Features of lung ADC cell lines used ( A ) and cytotoxic capacity of CD8 + T cells transduced with the three different constructs ( B ). In (A), total RNA was isolated from cell lines and negative control (A549 cell line) as indicated, and RT-PCR results obtained. The mean of triplicates is shown (A i ). Protein lysates from cell lines indicted and negative control A549 were immunoprecipitated with rabbit polyclonal anti-human CT37 Ab HPA011284, proteins were separated by SDS-PAGE, transferred to a membrane, and CT37 blotted using rabbit polyclonal AP51690PU-N Ab and HRP-conjugated goat anti-rabbit IgG. Blots were detected by chemiluminescence using autoradiography films [Kodak BioMax Light; (Aii)]. In (B), retroviral transduction of effector (E) allogeneic CD8 + . For results presented in (Bi)–(Biii), six [ 51 Cr] release assays, with each variable in triplicate, were performed. After 2 cycles of 10 d stimulation in vitro, E cells were harvested, washed, counted, and plated to be tested at 16:1 E:T ratios. Target (T) lung ADC lines HLA-A*A-02:01– positive HCC2935 (Bi), and H1993 (Bii), and HLA-A*02:01– negative line H1299, were labeled with 200 μCi of Na 2 51 CrO 4 for 1 h and washed. [ 51 Cr]-released cytotoxic assay proceed for 4 h at a 16:1 E:T ratio. Cultures proceeded without or with an anti–HLA-A2 Ab to block the HLA-A*02:01 molecule, and at the indicated concentrations. The HCC2935 cell line carries wild type (wt) TP53 and KRAS genes, and an EGFR 2237_2254del10 (deletion E746-S752). The H1993 line carries a TP53 726C > G (C242W) substitution, and wt EGFR and KRAS genes. The H1299 carries a TP53 gene deletion, and wt EGFR and KRAS genes. Data on TP53 . We genotyped mutations in the EGFR and KRAS . The p values from Mann–Whitney comparison of two means are presented.

    Article Snippet: To determine the identity of CD3 chains, immunoprecipitated proteins obtained as indicated above from cells transfected with construct 3 were blotted using rabbit anti-CD3δ EP4426, anti-CD3γ 134684, anti-CD3ε 133628, and Armenian hamster anti-CD3ζ H136-968 Abs (Abcam), 1:2000 HRP-conjugated goat anti-rabbit IgG or 1:000 HPR-conjugated rabbit anti-Armenian hamster IgG (Abcam), and the Immun-Star HRP kit, and blots were revealed by chemiluminescence using autoradiography Kodak BioMax Light film.

    Techniques: Transduction, Construct, Isolation, Negative Control, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, SDS Page, Autoradiography, In Vitro, Labeling, Blocking Assay, MANN-WHITNEY

    Effect of MTOM-adjuvanted WH121 protein on serum antibody levels in immunized mice . Six weeks after final immunization, the levels of serum IgG, IgG1, and IgG2c (replaced with IgG2a) antibodies from immunized mice were detected using ELISA. (A) WH121/MTOM induced IgG, IgG1, and IgG2a antibodies specific to WH121. (B) Comparison of antibody levels induced by WH121/MTO, WH121/Mv, and WH121/MTOM. Results are shown as mean (±SEM) log 10 end point titers and the ratio of IgG2a:IgG1 in the differently vaccinated groups ( n = 3).

    Journal: Frontiers in Immunology

    Article Title: A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2+ T Cells

    doi: 10.3389/fimmu.2017.00585

    Figure Lengend Snippet: Effect of MTOM-adjuvanted WH121 protein on serum antibody levels in immunized mice . Six weeks after final immunization, the levels of serum IgG, IgG1, and IgG2c (replaced with IgG2a) antibodies from immunized mice were detected using ELISA. (A) WH121/MTOM induced IgG, IgG1, and IgG2a antibodies specific to WH121. (B) Comparison of antibody levels induced by WH121/MTO, WH121/Mv, and WH121/MTOM. Results are shown as mean (±SEM) log 10 end point titers and the ratio of IgG2a:IgG1 in the differently vaccinated groups ( n = 3).

    Article Snippet: Horseradish peroxidase-conjugated rabbit anti-mouse IgG (1/5,000; Abcam, UK, Cat. no. Ab6789), IgG1 (1/10,000; Abcam, UK, Cat. no. Ab97240), or IgG2c (1/10,000; Abcam, UK, Cat. no. Ab97255) were added to the plates.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay