horseradish peroxidase conjugated secondary antibodies  (Beyotime)

 
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    Beyotime horseradish peroxidase conjugated secondary antibodies
    Horseradish Peroxidase Conjugated Secondary Antibodies, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated secondary antibodies/product/Beyotime
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated secondary antibodies - by Bioz Stars, 2020-07
    92/100 stars

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    Incubation:

    Article Title: The tripartite motif-containing protein 3 on the proliferation and cytokine secretion of rheumatoid arthritis fibroblast-like synoviocytes
    Article Snippet: .. After being washed three times in TBST, horseradish peroxidase-conjugated secondary antibodies (Beyotime Institute of Biotechnology, Shanghai, China) were added and incubated for 1 h at 25°C. .. After three washes in TBST, hybridized bands were detected using the ECL detection kit (Millipore Corp.).

    Article Title: Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIs
    Article Snippet: .. After being blocked with 5% non-fat dry milk (w/v) in Tris-buffered saline with 0.1% Tween-20, the membranes were incubated with anti–SP-C (1:200) or anti-T1α (1:200) primary antibodies (Santa Cruz Biotechnology) at 4C overnight and then incubated with a horseradish peroxidase–conjugated secondary antibody (Beyotime Institute of Biotechnology) at room temperature for 1 hr. ..

    Article Title: MiR-138 protects cardiac cells against hypoxia through modulation of glucose metabolism by targetting pyruvate dehydrogenase kinase 1
    Article Snippet: .. After blocking by 5% nonfat milk for 1 h at room temperature, membranes were probed with primary antibodies at 4°C overnight, followed by incubation with horseradish peroxidase conjugated secondary antibodies (Beyotime, China). .. After washing completely, membranes were detected by ECL method (Beyotime, China).

    Blocking Assay:

    Article Title: MiR-138 protects cardiac cells against hypoxia through modulation of glucose metabolism by targetting pyruvate dehydrogenase kinase 1
    Article Snippet: .. After blocking by 5% nonfat milk for 1 h at room temperature, membranes were probed with primary antibodies at 4°C overnight, followed by incubation with horseradish peroxidase conjugated secondary antibodies (Beyotime, China). .. After washing completely, membranes were detected by ECL method (Beyotime, China).

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    Beyotime hrp conjugated goat anti rabbit
    Specificities of anti-GnRHR1 and anti-GnRHR2 antisera against recombinant proteins as determined by Western blot analysis. The recombinant proteins (50 ng) or COS-7 cell extracts (100 μg) were separated on 12% SDS-PAGE gels, processed routinely, and immnoreacted with the rabbit anti-GnRHR1 antiserum [1:2,000 dilution; (A) ], the mouse anti-GnRHR2 antiserum [1:1,000 dilution; (B) ], the anti-GnRHR1 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (C) , or the anti-GnRHR2 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) . The secondary antibody was 1:1,000 diluted horseradish peroxidase <t>(HRP)-conjugated</t> goat anti-rabbit or anti-mouse <t>IgG</t> (H+L) (Beyotime, Shanghai, China), and the blots were visualized using BeyoECL Plus kit (Beyotime). GnRHR1-antigen, the recombinant GnRHR1 polypeptide used to immunize rabbit; GnRHR1-TRX, the region corresponding to GnRHR1 antigen expressed as thioredoxin (TRX) fusion protein; GnRHR2-TRX, GnRHR2 polypeptide region encompassing the synthetic antigen peptide expressed as TRX fusion protein. GnRHR1 and GnRHR2, the recombinant full-length ricefield eel GnRHR1 and GnRHR2 proteins expressed in transiently transfected COS-7 cells. pcDNA, extracts of COS-7 cells transfected with the empty vector pcDNA3.0. COS-7: extracts of COS-7 cells.
    Hrp Conjugated Goat Anti Rabbit, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti rabbit/product/Beyotime
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti rabbit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Beyotime peroxidase conjugated secondary antibody
    Specificities of anti-GnRHR1 and anti-GnRHR2 antisera against recombinant proteins as determined by Western blot analysis. The recombinant proteins (50 ng) or COS-7 cell extracts (100 μg) were separated on 12% SDS-PAGE gels, processed routinely, and immnoreacted with the rabbit anti-GnRHR1 antiserum [1:2,000 dilution; (A) ], the mouse anti-GnRHR2 antiserum [1:1,000 dilution; (B) ], the anti-GnRHR1 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (C) , or the anti-GnRHR2 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) . The secondary antibody was 1:1,000 diluted horseradish peroxidase <t>(HRP)-conjugated</t> goat anti-rabbit or anti-mouse <t>IgG</t> (H+L) (Beyotime, Shanghai, China), and the blots were visualized using BeyoECL Plus kit (Beyotime). GnRHR1-antigen, the recombinant GnRHR1 polypeptide used to immunize rabbit; GnRHR1-TRX, the region corresponding to GnRHR1 antigen expressed as thioredoxin (TRX) fusion protein; GnRHR2-TRX, GnRHR2 polypeptide region encompassing the synthetic antigen peptide expressed as TRX fusion protein. GnRHR1 and GnRHR2, the recombinant full-length ricefield eel GnRHR1 and GnRHR2 proteins expressed in transiently transfected COS-7 cells. pcDNA, extracts of COS-7 cells transfected with the empty vector pcDNA3.0. COS-7: extracts of COS-7 cells.
    Peroxidase Conjugated Secondary Antibody, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated secondary antibody/product/Beyotime
    Average 92 stars, based on 88 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    89
    Beyotime anti rabbit mouse horseradish peroxidase conjugated secondary antibody
    Specificities of anti-GnRHR1 and anti-GnRHR2 antisera against recombinant proteins as determined by Western blot analysis. The recombinant proteins (50 ng) or COS-7 cell extracts (100 μg) were separated on 12% SDS-PAGE gels, processed routinely, and immnoreacted with the rabbit anti-GnRHR1 antiserum [1:2,000 dilution; (A) ], the mouse anti-GnRHR2 antiserum [1:1,000 dilution; (B) ], the anti-GnRHR1 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (C) , or the anti-GnRHR2 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) . The secondary antibody was 1:1,000 diluted horseradish peroxidase <t>(HRP)-conjugated</t> goat anti-rabbit or anti-mouse <t>IgG</t> (H+L) (Beyotime, Shanghai, China), and the blots were visualized using BeyoECL Plus kit (Beyotime). GnRHR1-antigen, the recombinant GnRHR1 polypeptide used to immunize rabbit; GnRHR1-TRX, the region corresponding to GnRHR1 antigen expressed as thioredoxin (TRX) fusion protein; GnRHR2-TRX, GnRHR2 polypeptide region encompassing the synthetic antigen peptide expressed as TRX fusion protein. GnRHR1 and GnRHR2, the recombinant full-length ricefield eel GnRHR1 and GnRHR2 proteins expressed in transiently transfected COS-7 cells. pcDNA, extracts of COS-7 cells transfected with the empty vector pcDNA3.0. COS-7: extracts of COS-7 cells.
    Anti Rabbit Mouse Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Beyotime, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit mouse horseradish peroxidase conjugated secondary antibody/product/Beyotime
    Average 89 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anti rabbit mouse horseradish peroxidase conjugated secondary antibody - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    91
    Beyotime hrp conjugated goat anti rabbit igg secondary antibody
    Specificities of anti-GnRHR1 and anti-GnRHR2 antisera against recombinant proteins as determined by Western blot analysis. The recombinant proteins (50 ng) or COS-7 cell extracts (100 μg) were separated on 12% SDS-PAGE gels, processed routinely, and immnoreacted with the rabbit anti-GnRHR1 antiserum [1:2,000 dilution; (A) ], the mouse anti-GnRHR2 antiserum [1:1,000 dilution; (B) ], the anti-GnRHR1 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (C) , or the anti-GnRHR2 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) . The secondary antibody was 1:1,000 diluted horseradish peroxidase <t>(HRP)-conjugated</t> goat anti-rabbit or anti-mouse <t>IgG</t> (H+L) (Beyotime, Shanghai, China), and the blots were visualized using BeyoECL Plus kit (Beyotime). GnRHR1-antigen, the recombinant GnRHR1 polypeptide used to immunize rabbit; GnRHR1-TRX, the region corresponding to GnRHR1 antigen expressed as thioredoxin (TRX) fusion protein; GnRHR2-TRX, GnRHR2 polypeptide region encompassing the synthetic antigen peptide expressed as TRX fusion protein. GnRHR1 and GnRHR2, the recombinant full-length ricefield eel GnRHR1 and GnRHR2 proteins expressed in transiently transfected COS-7 cells. pcDNA, extracts of COS-7 cells transfected with the empty vector pcDNA3.0. COS-7: extracts of COS-7 cells.
    Hrp Conjugated Goat Anti Rabbit Igg Secondary Antibody, supplied by Beyotime, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti rabbit igg secondary antibody/product/Beyotime
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti rabbit igg secondary antibody - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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    Specificities of anti-GnRHR1 and anti-GnRHR2 antisera against recombinant proteins as determined by Western blot analysis. The recombinant proteins (50 ng) or COS-7 cell extracts (100 μg) were separated on 12% SDS-PAGE gels, processed routinely, and immnoreacted with the rabbit anti-GnRHR1 antiserum [1:2,000 dilution; (A) ], the mouse anti-GnRHR2 antiserum [1:1,000 dilution; (B) ], the anti-GnRHR1 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (C) , or the anti-GnRHR2 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) . The secondary antibody was 1:1,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (Beyotime, Shanghai, China), and the blots were visualized using BeyoECL Plus kit (Beyotime). GnRHR1-antigen, the recombinant GnRHR1 polypeptide used to immunize rabbit; GnRHR1-TRX, the region corresponding to GnRHR1 antigen expressed as thioredoxin (TRX) fusion protein; GnRHR2-TRX, GnRHR2 polypeptide region encompassing the synthetic antigen peptide expressed as TRX fusion protein. GnRHR1 and GnRHR2, the recombinant full-length ricefield eel GnRHR1 and GnRHR2 proteins expressed in transiently transfected COS-7 cells. pcDNA, extracts of COS-7 cells transfected with the empty vector pcDNA3.0. COS-7: extracts of COS-7 cells.

    Journal: Frontiers in Endocrinology

    Article Title: A Type IIb, but Not Type IIa, GnRH Receptor Mediates GnRH-Induced Release of Growth Hormone in the Ricefield Eel

    doi: 10.3389/fendo.2018.00721

    Figure Lengend Snippet: Specificities of anti-GnRHR1 and anti-GnRHR2 antisera against recombinant proteins as determined by Western blot analysis. The recombinant proteins (50 ng) or COS-7 cell extracts (100 μg) were separated on 12% SDS-PAGE gels, processed routinely, and immnoreacted with the rabbit anti-GnRHR1 antiserum [1:2,000 dilution; (A) ], the mouse anti-GnRHR2 antiserum [1:1,000 dilution; (B) ], the anti-GnRHR1 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (C) , or the anti-GnRHR2 antiserum pre-adsorbed with 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) . The secondary antibody was 1:1,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (Beyotime, Shanghai, China), and the blots were visualized using BeyoECL Plus kit (Beyotime). GnRHR1-antigen, the recombinant GnRHR1 polypeptide used to immunize rabbit; GnRHR1-TRX, the region corresponding to GnRHR1 antigen expressed as thioredoxin (TRX) fusion protein; GnRHR2-TRX, GnRHR2 polypeptide region encompassing the synthetic antigen peptide expressed as TRX fusion protein. GnRHR1 and GnRHR2, the recombinant full-length ricefield eel GnRHR1 and GnRHR2 proteins expressed in transiently transfected COS-7 cells. pcDNA, extracts of COS-7 cells transfected with the empty vector pcDNA3.0. COS-7: extracts of COS-7 cells.

    Article Snippet: Then slides were applied to the secondary antibody, HRP-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (1:1,000 dilution; Beyotime), and finally visualized with 3,3'-diaminobenzidine (DAB) solution, mounted, and digitally photographed with a microscope (Eclipse Ni-U, Nikon, Japan).

    Techniques: Recombinant, Western Blot, SDS Page, Transfection, Plasmid Preparation

    Specificities of GnRHR1 and GnRHR2 immunoreactivities in the pituitary of male ricefield eels as determined by immunohistochemical analysis. Sagittal sections of the pituitary gland were immunoreacted with the rabbit anti-GnRHR1 antiserum [1:500 dilution; (A) ], the pre-adsorbed anti-GnRHR1 antiserum by 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (B) , the mouse anti-GnRHR2 antiserum [1:500 dilution; (C) ], the pre-adsorbed anti-GnRHR2 antiserum by 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) , or the mixture of the rabbit anti-GnRHR1 (1:500 dilution) with the mouse anti-GnRHR2 (1:500 dilution) antisera (E,F) . The secondary antibody was 1:1,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (Beyotime, Shanghai, China) for (A–D) , and the mixture of Alexa Flour 488-labeled goat anti-rabbit IgG (H+L) (1:500 dilution) and Cy3-labeled goat anti-mouse IgG (H+L) (1:500 dilution) for (E) . DAPI was used to stain the nuclei blue. Immunostaining (brown) in (A–D) was visualized by the DAB chromogen and counterstained with hematoxylin. The black triangles and stars indicate the adenohypophysis and neurohypophysis tissues, respectively. The image of (E) was captured and analyzed with a Nikon i-E confocal microscope equipped with a CSU-W1 spinning-disk head (Yokogawa, Tokyo, Japan) for immunofluorescent staining of GnRHR1 (green) and GnRHR2 (red). (F) is the higher magnification of the boxed area in (E) . Sagittal sections of ricefield eel pituitary glands were shown here with the rostral (anterior) to the left. Scale bar = 50 μm except the image of (F) was 10 μm.

    Journal: Frontiers in Endocrinology

    Article Title: A Type IIb, but Not Type IIa, GnRH Receptor Mediates GnRH-Induced Release of Growth Hormone in the Ricefield Eel

    doi: 10.3389/fendo.2018.00721

    Figure Lengend Snippet: Specificities of GnRHR1 and GnRHR2 immunoreactivities in the pituitary of male ricefield eels as determined by immunohistochemical analysis. Sagittal sections of the pituitary gland were immunoreacted with the rabbit anti-GnRHR1 antiserum [1:500 dilution; (A) ], the pre-adsorbed anti-GnRHR1 antiserum by 10 ug/mL of recombinant GnRHR1 expressed in transfected COS-7 cells (B) , the mouse anti-GnRHR2 antiserum [1:500 dilution; (C) ], the pre-adsorbed anti-GnRHR2 antiserum by 10 ug/mL of recombinant GnRHR2 expressed in transfected COS-7 cells (D) , or the mixture of the rabbit anti-GnRHR1 (1:500 dilution) with the mouse anti-GnRHR2 (1:500 dilution) antisera (E,F) . The secondary antibody was 1:1,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (Beyotime, Shanghai, China) for (A–D) , and the mixture of Alexa Flour 488-labeled goat anti-rabbit IgG (H+L) (1:500 dilution) and Cy3-labeled goat anti-mouse IgG (H+L) (1:500 dilution) for (E) . DAPI was used to stain the nuclei blue. Immunostaining (brown) in (A–D) was visualized by the DAB chromogen and counterstained with hematoxylin. The black triangles and stars indicate the adenohypophysis and neurohypophysis tissues, respectively. The image of (E) was captured and analyzed with a Nikon i-E confocal microscope equipped with a CSU-W1 spinning-disk head (Yokogawa, Tokyo, Japan) for immunofluorescent staining of GnRHR1 (green) and GnRHR2 (red). (F) is the higher magnification of the boxed area in (E) . Sagittal sections of ricefield eel pituitary glands were shown here with the rostral (anterior) to the left. Scale bar = 50 μm except the image of (F) was 10 μm.

    Article Snippet: Then slides were applied to the secondary antibody, HRP-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (1:1,000 dilution; Beyotime), and finally visualized with 3,3'-diaminobenzidine (DAB) solution, mounted, and digitally photographed with a microscope (Eclipse Ni-U, Nikon, Japan).

    Techniques: Immunohistochemistry, Recombinant, Transfection, Labeling, Staining, Immunostaining, Microscopy

    GnRH1, GnRH2, and GnRH3 immunostaining in the brain and pituitary of ricefield eels. Sagittal sections of the brains together with the pituitary gland of ricefield eels were immunoreacted with primary antibodies, the rabbit polyclonal antibody AS-691 for GnRH1 [1:7,000 dilution; (B,C,H) ], the rabbit polyclonal antibody 675 for GnRH2 [1:2,000 dilution; (D,I) ], or the mouse monoclonal antibody LRH13 for GnRH3 [1:2,000 dilution; (E–G,J) ]. After incubation with primary antibodies for 40 h at 4°C, sections were then exposed to the secondary antibody, HRP-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (1:1,000 dilution; Beyotime, Shanghai, China), and finally visualized with 3,3'-diaminobenzidine (DAB) solution, mounted, and digitally photographed with a Nikon Eclipse Ni-U microscope (Japan). (A) , the schematic diagram of the ricefield eel brain and pituitary. Red, green, and blue dots in (A) represent GnRH1, GnRH2, and GnRH3 immunoreactive neurons, respectively. OB, olfactory bulbs; TEL, telencephalon; VT, ventral telencephalon; POA, preoptic area; OT, optic tectum-thalamus; MT, midbrain tegmentum; Hyp, hypothalamus; Pit, pituitary; Ce, cerebellum; MO, medulla oblongata. Scale bar = 50 μm except where it is specifically designated otherwise.

    Journal: Frontiers in Endocrinology

    Article Title: A Type IIb, but Not Type IIa, GnRH Receptor Mediates GnRH-Induced Release of Growth Hormone in the Ricefield Eel

    doi: 10.3389/fendo.2018.00721

    Figure Lengend Snippet: GnRH1, GnRH2, and GnRH3 immunostaining in the brain and pituitary of ricefield eels. Sagittal sections of the brains together with the pituitary gland of ricefield eels were immunoreacted with primary antibodies, the rabbit polyclonal antibody AS-691 for GnRH1 [1:7,000 dilution; (B,C,H) ], the rabbit polyclonal antibody 675 for GnRH2 [1:2,000 dilution; (D,I) ], or the mouse monoclonal antibody LRH13 for GnRH3 [1:2,000 dilution; (E–G,J) ]. After incubation with primary antibodies for 40 h at 4°C, sections were then exposed to the secondary antibody, HRP-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (1:1,000 dilution; Beyotime, Shanghai, China), and finally visualized with 3,3'-diaminobenzidine (DAB) solution, mounted, and digitally photographed with a Nikon Eclipse Ni-U microscope (Japan). (A) , the schematic diagram of the ricefield eel brain and pituitary. Red, green, and blue dots in (A) represent GnRH1, GnRH2, and GnRH3 immunoreactive neurons, respectively. OB, olfactory bulbs; TEL, telencephalon; VT, ventral telencephalon; POA, preoptic area; OT, optic tectum-thalamus; MT, midbrain tegmentum; Hyp, hypothalamus; Pit, pituitary; Ce, cerebellum; MO, medulla oblongata. Scale bar = 50 μm except where it is specifically designated otherwise.

    Article Snippet: Then slides were applied to the secondary antibody, HRP-conjugated goat anti-rabbit or anti-mouse IgG (H+L) (1:1,000 dilution; Beyotime), and finally visualized with 3,3'-diaminobenzidine (DAB) solution, mounted, and digitally photographed with a microscope (Eclipse Ni-U, Nikon, Japan).

    Techniques: Immunostaining, Incubation, Microscopy