horseradish peroxidase conjugated goat anti murine igg  (Thermo Fisher)


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    Name:
    Horseradish Peroxidase HRP EIA Grade
    Description:
    This highly purified preparation of horseradish peroxidase is useful as a protein tracer in histochemistry as well as cytochemistry and also as a valuable experimental tool in hodological neurography Also the enzyme preparation has been used as an enzyme label in enzyme immunoassays
    Catalog Number:
    012001
    Price:
    None
    Applications:
    Cell Analysis|Cellular Imaging|IHC Staining & Detection|Immunocytochemistry (ICC)|Immunofluorescence (IF)|Immunohistochemistry (IHC)|Labeling Proteins & Antibodies with Enzymes & Proteins|Protein Biology|Protein Labeling|Protein Labeling & Crosslinking|Protein, Peptide & Antibody Labeling|Labeling Chemistry
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher horseradish peroxidase conjugated goat anti murine igg
    Depolymerizing actin filaments in suspended neutrophils does not prevent β 2 integrin extension by soluble P-selectin or chemokine but impairs hybrid domain swing out by soluble chemokine. A, DMSO- or latrunculin B ( Lat B )-treated human neutrophils were incubated with platelet-derived P-selectin in buffer containing Ca 2+ or EDTA. The cells were incubated with <t>mAb</t> IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse <t>IgG</t> and analyzed by flow cytometry. B, untreated human neutrophils were incubated with isotope control mAb or mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG. Alternatively, DMSO-, latrunculin B-, or blebbistatin ( Blebb )-treated human neutrophils were stimulated with IL-8, incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG, and analyzed by flow cytometry. The data are representative of three experiments.
    This highly purified preparation of horseradish peroxidase is useful as a protein tracer in histochemistry as well as cytochemistry and also as a valuable experimental tool in hodological neurography Also the enzyme preparation has been used as an enzyme label in enzyme immunoassays
    https://www.bioz.com/result/horseradish peroxidase conjugated goat anti murine igg/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated goat anti murine igg - by Bioz Stars, 2020-08
    99/100 stars

    Related Products / Commonly Used Together

    murine anti-moesin mab

    Images

    1) Product Images from "Signal-dependent Slow Leukocyte Rolling Does Not Require Cytoskeletal Anchorage of P-selectin Glycoprotein Ligand-1 (PSGL-1) or Integrin ?L?2 *"

    Article Title: Signal-dependent Slow Leukocyte Rolling Does Not Require Cytoskeletal Anchorage of P-selectin Glycoprotein Ligand-1 (PSGL-1) or Integrin ?L?2 *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.361519

    Depolymerizing actin filaments in suspended neutrophils does not prevent β 2 integrin extension by soluble P-selectin or chemokine but impairs hybrid domain swing out by soluble chemokine. A, DMSO- or latrunculin B ( Lat B )-treated human neutrophils were incubated with platelet-derived P-selectin in buffer containing Ca 2+ or EDTA. The cells were incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG and analyzed by flow cytometry. B, untreated human neutrophils were incubated with isotope control mAb or mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG. Alternatively, DMSO-, latrunculin B-, or blebbistatin ( Blebb )-treated human neutrophils were stimulated with IL-8, incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG, and analyzed by flow cytometry. The data are representative of three experiments.
    Figure Legend Snippet: Depolymerizing actin filaments in suspended neutrophils does not prevent β 2 integrin extension by soluble P-selectin or chemokine but impairs hybrid domain swing out by soluble chemokine. A, DMSO- or latrunculin B ( Lat B )-treated human neutrophils were incubated with platelet-derived P-selectin in buffer containing Ca 2+ or EDTA. The cells were incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG and analyzed by flow cytometry. B, untreated human neutrophils were incubated with isotope control mAb or mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG. Alternatively, DMSO-, latrunculin B-, or blebbistatin ( Blebb )-treated human neutrophils were stimulated with IL-8, incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG, and analyzed by flow cytometry. The data are representative of three experiments.

    Techniques Used: Incubation, Derivative Assay, Flow Cytometry, Cytometry

    Related Articles

    Western Blot:

    Article Title: Vacuolar Na+/H+ antiporter cation selectivity is regulated by calmodulin from within the vacuole in a Ca2+- and pH-dependent manner
    Article Snippet: .. Proteins were resolved with 12% SDS/PAGE and analyzed by Western blot analyses using a FLAG M2 antibody (Sigma-Aldrich) and anti-mouse IgG antibody horseradish peroxidase conjugate (Molecular Probes). .. The blots were visualized by enhanced chemioluminiscence (ECL) plus Western blotting detection reagents (Amersham Biosciences).

    Article Title: Connecting Replication and Repair: YoaA, a Helicase-Related Protein, Promotes Azidothymidine Tolerance through Association with Chi, an Accessory Clamp Loader Protein
    Article Snippet: .. The Western blot analysis was performed using the QIAexpress detection kit and protocol (Qiagen) and using detection of BBD-YoaA with a 1:10,000 dilution of NeutrAvidin Protein Horseradish Peroxidase Conjugated antibody (Pierce). ..

    Incubation:

    Article Title: Identification of Host-Immune Response Protein Candidates in the Sera of Human Oral Squamous Cell Carcinoma Patients
    Article Snippet: .. Following washing, membranes were incubated for 1 hour at room temperature with horseradish peroxidase (HRP)-linked monoclonal anti-human immunoglobulin M (IgM) (1∶5000; Invitrogen, USA). .. The membranes were again washed and then visualized using chemiluminescence substrate (Pierce, USA) and 18 cm × 24 cm films (Kodak, USA).

    SDS Page:

    Article Title: Vacuolar Na+/H+ antiporter cation selectivity is regulated by calmodulin from within the vacuole in a Ca2+- and pH-dependent manner
    Article Snippet: .. Proteins were resolved with 12% SDS/PAGE and analyzed by Western blot analyses using a FLAG M2 antibody (Sigma-Aldrich) and anti-mouse IgG antibody horseradish peroxidase conjugate (Molecular Probes). .. The blots were visualized by enhanced chemioluminiscence (ECL) plus Western blotting detection reagents (Amersham Biosciences).

    Conjugation Assay:

    Article Title: Anti-c-Met antibodies recognising a temperature sensitive epitope, inhibit cell growth
    Article Snippet: .. HRP conjugation to seeMet antibodies was performed using EZ-link Plus Activated Peroxidase (Pierce/Thermo Scientific). .. HRP conjugated antibodies were used at 1 µg/mL on Western blotting.

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  • 99
    Thermo Fisher horseradish peroxidase conjugated goat anti murine igg
    Depolymerizing actin filaments in suspended neutrophils does not prevent β 2 integrin extension by soluble P-selectin or chemokine but impairs hybrid domain swing out by soluble chemokine. A, DMSO- or latrunculin B ( Lat B )-treated human neutrophils were incubated with platelet-derived P-selectin in buffer containing Ca 2+ or EDTA. The cells were incubated with <t>mAb</t> IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse <t>IgG</t> and analyzed by flow cytometry. B, untreated human neutrophils were incubated with isotope control mAb or mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG. Alternatively, DMSO-, latrunculin B-, or blebbistatin ( Blebb )-treated human neutrophils were stimulated with IL-8, incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG, and analyzed by flow cytometry. The data are representative of three experiments.
    Horseradish Peroxidase Conjugated Goat Anti Murine Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase conjugated goat anti murine igg/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase conjugated goat anti murine igg - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    88
    Thermo Fisher hrp conjugated goat anti murine igg
    Depolymerizing actin filaments in suspended neutrophils does not prevent β 2 integrin extension by soluble P-selectin or chemokine but impairs hybrid domain swing out by soluble chemokine. A, DMSO- or latrunculin B ( Lat B )-treated human neutrophils were incubated with platelet-derived P-selectin in buffer containing Ca 2+ or EDTA. The cells were incubated with <t>mAb</t> IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse <t>IgG</t> and analyzed by flow cytometry. B, untreated human neutrophils were incubated with isotope control mAb or mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG. Alternatively, DMSO-, latrunculin B-, or blebbistatin ( Blebb )-treated human neutrophils were stimulated with IL-8, incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG, and analyzed by flow cytometry. The data are representative of three experiments.
    Hrp Conjugated Goat Anti Murine Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti murine igg/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti murine igg - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results


    Depolymerizing actin filaments in suspended neutrophils does not prevent β 2 integrin extension by soluble P-selectin or chemokine but impairs hybrid domain swing out by soluble chemokine. A, DMSO- or latrunculin B ( Lat B )-treated human neutrophils were incubated with platelet-derived P-selectin in buffer containing Ca 2+ or EDTA. The cells were incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG and analyzed by flow cytometry. B, untreated human neutrophils were incubated with isotope control mAb or mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG. Alternatively, DMSO-, latrunculin B-, or blebbistatin ( Blebb )-treated human neutrophils were stimulated with IL-8, incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG, and analyzed by flow cytometry. The data are representative of three experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Signal-dependent Slow Leukocyte Rolling Does Not Require Cytoskeletal Anchorage of P-selectin Glycoprotein Ligand-1 (PSGL-1) or Integrin ?L?2 *

    doi: 10.1074/jbc.M112.361519

    Figure Lengend Snippet: Depolymerizing actin filaments in suspended neutrophils does not prevent β 2 integrin extension by soluble P-selectin or chemokine but impairs hybrid domain swing out by soluble chemokine. A, DMSO- or latrunculin B ( Lat B )-treated human neutrophils were incubated with platelet-derived P-selectin in buffer containing Ca 2+ or EDTA. The cells were incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG and analyzed by flow cytometry. B, untreated human neutrophils were incubated with isotope control mAb or mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG. Alternatively, DMSO-, latrunculin B-, or blebbistatin ( Blebb )-treated human neutrophils were stimulated with IL-8, incubated with mAb IB4, KIM127, or MEM148 and then with PE-conjugated anti-mouse IgG, and analyzed by flow cytometry. The data are representative of three experiments.

    Article Snippet: Murine anti-moesin mAb (clone 38/87) and horseradish peroxidase-conjugated goat anti-murine IgG were from Thermo Scientific (Fremont, CA).

    Techniques: Incubation, Derivative Assay, Flow Cytometry, Cytometry