horse polyclonal  (Cell Signaling Technology Inc)

Journal: ACS Omega

Article Title: Inhibition of Hepatitis B Virus Replication by a Novel GalNAc–siRNA In Vivo and In Vitro

doi: 10.1021/acsomega.4c06840

Figure Lengend Snippet: Life cycle of HBV. NTCP, Na + -taurocholate cotransport polypeptide; cccDNA, covalently closed circular DNA; rcDNA, relaxed circular DNA; dslDNA, double-stranded linear DNA; pgRNA, multivalent guide RNA; HBcAg, HBV core antigen; HBsAg, hepatitis B surface antigen; HBX, the X antigen encoded by hepatitis B; HBeAg, hepatitis Be antigen; cRNA, cytoplasmic RNA.

Article Snippet: The main reagents and instruments used in this research experiment were as follows: QIAamp 96 DNA Blood Kit (Qiagen, 51162), FastStart Universal Probe Master (Roche, 04914058001), FastQuant RT Kit (TianGen, KR106), TRIZOL (Invitrogen, 15596018), Gene Expression Assay (GAPDH) (AB, AB-Mm99999915), anti-HBs ELISA kit (Autobio, CL0311), BCA Protein Assay Kit (Thermo, Vl311907), polyclonal horse anti-HBsAg antibody(Abcam, ab9193), rabbit anti-horse IgG H&L (HRP) (Abcam, ab6921), ImmPACTTM DAB peroxidase substrate (Vector, SK-4105), AST Activity Assay Kit (Beckman, OSR6109), ALT Activity Assay Kit (Beckman, OSR6107), microplate reader (BioTek, Spectra max 340PC), fast real-time PCR system (Applied Biosystems, 7900HT Fast Real-time PCR system), spectrophotometer (Thermo Scientific, NANODROP 1000), centrifuge (Beckman, Allegra-X15R), embedding machine (Leica, Histo core Arcadia H), digital film scanner (Leica, Aperio AT2), and automatic biochemical analyzer (Beckman, AU480).

Techniques:

Journal: ACS Omega

Article Title: Inhibition of Hepatitis B Virus Replication by a Novel GalNAc–siRNA In Vivo and In Vitro

doi: 10.1021/acsomega.4c06840

Figure Lengend Snippet: Fitting curves for the tested compound’s inhibitory activity against HBV (HBV DNA, HBsAg, HBeAg, and HBV RNA); data were presented as mean ± SD; each concentration point was assessed in triplicate.

Article Snippet: The main reagents and instruments used in this research experiment were as follows: QIAamp 96 DNA Blood Kit (Qiagen, 51162), FastStart Universal Probe Master (Roche, 04914058001), FastQuant RT Kit (TianGen, KR106), TRIZOL (Invitrogen, 15596018), Gene Expression Assay (GAPDH) (AB, AB-Mm99999915), anti-HBs ELISA kit (Autobio, CL0311), BCA Protein Assay Kit (Thermo, Vl311907), polyclonal horse anti-HBsAg antibody(Abcam, ab9193), rabbit anti-horse IgG H&L (HRP) (Abcam, ab6921), ImmPACTTM DAB peroxidase substrate (Vector, SK-4105), AST Activity Assay Kit (Beckman, OSR6109), ALT Activity Assay Kit (Beckman, OSR6107), microplate reader (BioTek, Spectra max 340PC), fast real-time PCR system (Applied Biosystems, 7900HT Fast Real-time PCR system), spectrophotometer (Thermo Scientific, NANODROP 1000), centrifuge (Beckman, Allegra-X15R), embedding machine (Leica, Histo core Arcadia H), digital film scanner (Leica, Aperio AT2), and automatic biochemical analyzer (Beckman, AU480).

Techniques: Activity Assay, Concentration Assay

Journal: ACS Omega

Article Title: Inhibition of Hepatitis B Virus Replication by a Novel GalNAc–siRNA In Vivo and In Vitro

doi: 10.1021/acsomega.4c06840

Figure Lengend Snippet: In the HepG2 transient transfection model, inhibition curves for HBV HBsAg revealed that the IC 50 values of the HBV HBsAg biomarker for genotypes A, B, C, and D were 0.10, 0.11, 0.07, and 0.08 nM, respectively. Data were presented as mean ± SD; each concentration point was assessed in triplicate.

Article Snippet: The main reagents and instruments used in this research experiment were as follows: QIAamp 96 DNA Blood Kit (Qiagen, 51162), FastStart Universal Probe Master (Roche, 04914058001), FastQuant RT Kit (TianGen, KR106), TRIZOL (Invitrogen, 15596018), Gene Expression Assay (GAPDH) (AB, AB-Mm99999915), anti-HBs ELISA kit (Autobio, CL0311), BCA Protein Assay Kit (Thermo, Vl311907), polyclonal horse anti-HBsAg antibody(Abcam, ab9193), rabbit anti-horse IgG H&L (HRP) (Abcam, ab6921), ImmPACTTM DAB peroxidase substrate (Vector, SK-4105), AST Activity Assay Kit (Beckman, OSR6109), ALT Activity Assay Kit (Beckman, OSR6107), microplate reader (BioTek, Spectra max 340PC), fast real-time PCR system (Applied Biosystems, 7900HT Fast Real-time PCR system), spectrophotometer (Thermo Scientific, NANODROP 1000), centrifuge (Beckman, Allegra-X15R), embedding machine (Leica, Histo core Arcadia H), digital film scanner (Leica, Aperio AT2), and automatic biochemical analyzer (Beckman, AU480).

Techniques: Transfection, Inhibition, Biomarker Assay, Concentration Assay

Journal: ACS Omega

Article Title: Inhibition of Hepatitis B Virus Replication by a Novel GalNAc–siRNA In Vivo and In Vitro

doi: 10.1021/acsomega.4c06840

Figure Lengend Snippet: Impact of siRNA on HBsAg, HBeAg, and HBV DNA levels in the plasma of AAV/HBV mice. Data were presented as mean ± standard error of the mean (SEM) in each mice group (Group 1 to Group 3, n = 10; Group 4, n = 5 to 15).

Article Snippet: The main reagents and instruments used in this research experiment were as follows: QIAamp 96 DNA Blood Kit (Qiagen, 51162), FastStart Universal Probe Master (Roche, 04914058001), FastQuant RT Kit (TianGen, KR106), TRIZOL (Invitrogen, 15596018), Gene Expression Assay (GAPDH) (AB, AB-Mm99999915), anti-HBs ELISA kit (Autobio, CL0311), BCA Protein Assay Kit (Thermo, Vl311907), polyclonal horse anti-HBsAg antibody(Abcam, ab9193), rabbit anti-horse IgG H&L (HRP) (Abcam, ab6921), ImmPACTTM DAB peroxidase substrate (Vector, SK-4105), AST Activity Assay Kit (Beckman, OSR6109), ALT Activity Assay Kit (Beckman, OSR6107), microplate reader (BioTek, Spectra max 340PC), fast real-time PCR system (Applied Biosystems, 7900HT Fast Real-time PCR system), spectrophotometer (Thermo Scientific, NANODROP 1000), centrifuge (Beckman, Allegra-X15R), embedding machine (Leica, Histo core Arcadia H), digital film scanner (Leica, Aperio AT2), and automatic biochemical analyzer (Beckman, AU480).

Techniques:

Journal: eLife

Article Title: A transcription network underlies the dual genomic coordination of mitochondrial biogenesis

doi: 10.7554/eLife.96536

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-mouse IgG, HRP-linked (Horse polyclonal) , Cell Signaling , Cat# 7076; RRID: AB_330924 , WB (1:2000).

Techniques: Transgenic Assay, Recombinant, Plasmid Preparation, Cell Culture, Expressing, Sequencing, Real-time Polymerase Chain Reaction, Transfection, Sample Prep, Bicinchoninic Acid Protein Assay, SYBR Green Assay, Software, Imaging

Journal: Cell Reports Medicine

Article Title: Acutely blocking excessive mitochondrial fission prevents chronic neurodegeneration after traumatic brain injury

doi: 10.1016/j.xcrm.2024.101715

Figure Lengend Snippet:

Article Snippet: Horse polyclonal anti-mouse IgG, HRP-linked , Cell Signaling Technology , Cat# 7076; RRID: AB_330924.

Techniques: Transduction, Diagnostic Assay, Recombinant, Isolation, In Situ, Software