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Stratagene homologous recombination
Homologous Recombination, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/homologous recombination/product/Stratagene
Average 93 stars, based on 72 article reviews
Price from $9.99 to $1999.99
homologous recombination - by Bioz Stars, 2020-09
93/100 stars

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Article Title: Targeting of tumor radioiodine therapy by expression of the sodium iodide symporter under control of the survivin promoter
Article Snippet: Subsequently, homologous recombination was performed using the Adeasier system (Stratagene) in BJ5183 Escherichia coli to generate pAd-SUR-NIS.

Article Title: CD4+ T cell-independent DNA vaccination against opportunistic infections
Article Snippet: Adenovirus OVA (AdOVA) was generated from pCMVOVA after homologous recombination in E. coli with AdEasy-1 (Stratagene) ( ).

Article Title: Codon optimization of the adenoviral fiber negatively impacts structural protein expression and viral fitness
Article Snippet: The pXK3.1 plasmid contains two long arms complementary to the adenoviral genome at both fiber extremes. pAdFO was generated by homologous recombination of the pXK3.1-FO with the genome of the wild-type adenovirus in E. coli BJ5183 cells (Stratagene, Wilmington, NC) as described in .

Article Title: Persistent Down-Regulation of Fli1, a Suppressor of Collagen Transcription, in Fibrotic Scleroderma Skin
Article Snippet: For homologous recombination, Pme I digested shuttle plasmid bearing the kanamycin resistance gene was co-electroporated with the circular adenoviral genome plasmid pAdEasy-1 (E1A deleted) into competent BJ5183 bacterial cells (Stratagene) and plated on kanamycin media.

Article Title: Therapeutic efficacy of an oncolytic adenovirus containing RGD ligand in minor capsid protein IX and Fiber, ?24DoubleRGD, in an ovarian cancer model
Article Snippet: The pSlΔ24IXFlag45ÅRGD shuttle vector and the pTG3602 backbone ( ) were digested with PmeI and ClaI, respectively, and were subjected to homologous recombination in Escherichia coli ( E. coli ) strain BJ5183 (Stratagene, La Jolla, CA).

Article Title: An organellar maturase associates with multiple group II introns
Article Snippet: Vector pRZN+: The target region for homologous recombination was amplified from purified chloroplast DNA using primers 5′matKAu1 and 5′matKAu2 and cloned into pBluescript II SK+ (Stratagene) via SalI linkers.

Article Title: Suppressor of Cytokine Signaling-3 Is a Glucagon-inducible Inhibitor of PKA Activity and Gluconeogenic Gene Expression in Hepatocytes *
Article Snippet: Recombinant adenovirus was generated by homologous recombination of pShuttle-U6-shmSOCS3 or pShuttle-U6-shluciferase constructs with pAdEasy1 in BJ5183 cells following the manufacturer's protocol (Stratagene).

Article Title: Development of a method for effective amplification of human adenovirus 40
Article Snippet: The plasmid encoding pure Ad40wt was built by the homologous recombination between the entirety of Ad40 DNA and the rescue plasmids, which contained homology regions of Ad40-left and right hand-ends (left 1849 bp between 1 and 1849 of GenBank accession no. ; right 494 bp between 33721 and 34214), in E.coli BJ5183 (Stratagene, La Jolla, CA).

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    Stratagene dab2 homologous recombination targeting vector
    Increased cholesterol and low density lipoprotein (LDL) levels in <t>Dab2</t> deficient mice. Blood was collected for lipid analysis from 6 month old littermate control and Dab2 deficient mice with dab2 (df/df);Sox2-Cre genotype. The values are reported as the average +/- standard deviation. Student T-test indicates the significance, P
    Dab2 Homologous Recombination Targeting Vector, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dab2 homologous recombination targeting vector/product/Stratagene
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dab2 homologous recombination targeting vector - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    93
    Stratagene homologous recombination
    Increased cholesterol and low density lipoprotein (LDL) levels in <t>Dab2</t> deficient mice. Blood was collected for lipid analysis from 6 month old littermate control and Dab2 deficient mice with dab2 (df/df);Sox2-Cre genotype. The values are reported as the average +/- standard deviation. Student T-test indicates the significance, P
    Homologous Recombination, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/homologous recombination/product/Stratagene
    Average 93 stars, based on 72 article reviews
    Price from $9.99 to $1999.99
    homologous recombination - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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    Increased cholesterol and low density lipoprotein (LDL) levels in Dab2 deficient mice. Blood was collected for lipid analysis from 6 month old littermate control and Dab2 deficient mice with dab2 (df/df);Sox2-Cre genotype. The values are reported as the average +/- standard deviation. Student T-test indicates the significance, P

    Journal: BMC Developmental Biology

    Article Title: Differential requirement for Dab2 in the development of embryonic and extra-embryonic tissues

    doi: 10.1186/1471-213X-13-39

    Figure Lengend Snippet: Increased cholesterol and low density lipoprotein (LDL) levels in Dab2 deficient mice. Blood was collected for lipid analysis from 6 month old littermate control and Dab2 deficient mice with dab2 (df/df);Sox2-Cre genotype. The values are reported as the average +/- standard deviation. Student T-test indicates the significance, P

    Article Snippet: Construction of the dab2 homologous recombination targeting vector and conditional mutant mice Using the mouse Dab2 cDNA as a probe, three clones (λ20, λ24, and λ28) of mouse genomic DNA containing the dab2 gene were isolated from a 129/Sv genome library in λZap (Stratagene) [ ].

    Techniques: Mouse Assay, Standard Deviation

    Mild defective positioning phenotype in embryoid bodies and compensatory expression of Arh and Numb in Dab2 null ES cells and embryos. (A) In some of the dab2 (-/-) ES clones (such as clone dfg15), a monolayer epithelial structure is seen at some of the surface of embryoid bodies (indicated by arrows), as shown by an example of a section stained with Dab2 (green) (no signal in this image), GATA4 (red), and DAPI (blue). (B) Western blot shows that Arh and Numb were variably expressed in embryoid bodies derived from several ES cell clones of different dab2 genotypes. (C) The expression of Dab2, Arh, and Numb adaptors proteins was analyzed by Western blotting of E9.5 embryos from matings between female dab2 (fl/fl) and male dab2 (+/df); Sox2-Cre. The embryos were harvested, dissected free of extraembryonic tissues, lysed and denatured in SDS buffer, and probed for Dab2, Arh, and Numb. The extraembryonic tissues were used for genotyping.

    Journal: BMC Developmental Biology

    Article Title: Differential requirement for Dab2 in the development of embryonic and extra-embryonic tissues

    doi: 10.1186/1471-213X-13-39

    Figure Lengend Snippet: Mild defective positioning phenotype in embryoid bodies and compensatory expression of Arh and Numb in Dab2 null ES cells and embryos. (A) In some of the dab2 (-/-) ES clones (such as clone dfg15), a monolayer epithelial structure is seen at some of the surface of embryoid bodies (indicated by arrows), as shown by an example of a section stained with Dab2 (green) (no signal in this image), GATA4 (red), and DAPI (blue). (B) Western blot shows that Arh and Numb were variably expressed in embryoid bodies derived from several ES cell clones of different dab2 genotypes. (C) The expression of Dab2, Arh, and Numb adaptors proteins was analyzed by Western blotting of E9.5 embryos from matings between female dab2 (fl/fl) and male dab2 (+/df); Sox2-Cre. The embryos were harvested, dissected free of extraembryonic tissues, lysed and denatured in SDS buffer, and probed for Dab2, Arh, and Numb. The extraembryonic tissues were used for genotyping.

    Article Snippet: Construction of the dab2 homologous recombination targeting vector and conditional mutant mice Using the mouse Dab2 cDNA as a probe, three clones (λ20, λ24, and λ28) of mouse genomic DNA containing the dab2 gene were isolated from a 129/Sv genome library in λZap (Stratagene) [ ].

    Techniques: Expressing, Clone Assay, Staining, Western Blot, Derivative Assay

    Histological phenotypes of constitutively Dab2 null embryos at E4.5 and E5.5. (A) Fixed and paraffin embedded E4.5 embryos in utero from timed matings of dab2 (+/df) mice were sectioned and stained with H E to identify implanted embryos. Once found, the serial sections were used for staining with Dab2 to identify dab2 deletion mutants, adjacent sections were then stained with GATA4 and GATA6 as markers for primitive endoderm; and with Oct3/4 to identify cells of the inner cell mass. Consecutive sections of a representative Dab2-positive and a Dab2-negative ( dab2 (-/-)) E4.5 embryo are shown. (B) Examples of one wildtype (WT) and five Dab2-negative ( dab2 (-/-)) E5.5 embryos are shown. Dab2 staining was used to identify mutant embryos. An adjacent section was stained for GATA4 to identify the endoderm cells. The images shown were sections at or near midline (widest area) of the embryos. A spectrum of severity in disorganization of endoderm cells was seen in the dab2 null embryos. The arrow indicates a parietal endoderm cell in the wildtype.

    Journal: BMC Developmental Biology

    Article Title: Differential requirement for Dab2 in the development of embryonic and extra-embryonic tissues

    doi: 10.1186/1471-213X-13-39

    Figure Lengend Snippet: Histological phenotypes of constitutively Dab2 null embryos at E4.5 and E5.5. (A) Fixed and paraffin embedded E4.5 embryos in utero from timed matings of dab2 (+/df) mice were sectioned and stained with H E to identify implanted embryos. Once found, the serial sections were used for staining with Dab2 to identify dab2 deletion mutants, adjacent sections were then stained with GATA4 and GATA6 as markers for primitive endoderm; and with Oct3/4 to identify cells of the inner cell mass. Consecutive sections of a representative Dab2-positive and a Dab2-negative ( dab2 (-/-)) E4.5 embryo are shown. (B) Examples of one wildtype (WT) and five Dab2-negative ( dab2 (-/-)) E5.5 embryos are shown. Dab2 staining was used to identify mutant embryos. An adjacent section was stained for GATA4 to identify the endoderm cells. The images shown were sections at or near midline (widest area) of the embryos. A spectrum of severity in disorganization of endoderm cells was seen in the dab2 null embryos. The arrow indicates a parietal endoderm cell in the wildtype.

    Article Snippet: Construction of the dab2 homologous recombination targeting vector and conditional mutant mice Using the mouse Dab2 cDNA as a probe, three clones (λ20, λ24, and λ28) of mouse genomic DNA containing the dab2 gene were isolated from a 129/Sv genome library in λZap (Stratagene) [ ].

    Techniques: In Utero, Mouse Assay, Staining, Mutagenesis

    Conditional dab2 deletion by Meox2-Cre and Sox2-Cre transgene. Female dab2 (fl/fl) mice were crossed with male dab2 (df/+);Meox2-Cre or dab2 (df/+);Sox2-Cre mice to produce mosaic mutant mice without deletion in the extraembryonic endoderm. Mice of around the age of 3 months were used for these analyses. (A) An example of PCR genotyping assay using tail DNA of the progenies (#1 to 12) generated from the cross between dab2 (fl/fl) and dab2 (df/+);Meox2-Cre. Specific PCR amplifications to identify the locus for cre, fl, df, and wt were performed either separately or in a combined reaction. Examples of Dab2 immunostainings in kidney of a dab2 (df/fl) (B) and a dab2 (df/fl);Meox2-Cre (C) mouse are shown, with corresponding PCR genotyping results shown at the upper right corner. (D) An example of PCR genotyping assay using tail DNA of the progenies (#71 to 94) generated from crosses between dab2 (fl/fl) and dab2 (df/+);Sox2-Cre. (E) DNA gel resolving the genotyping PCR products shows a comparison of the deletion efficiency generated by Sox2-Cre or Meox2-Cre. Corresponding Dab2 stainings show the extent of Dab2 mosaicism in the kidney sections from dab2 (+/df) (F) , dab2 (fl/df);Sox2-Cre (G) , and dab2 (fl/df);Meox2-Cre (H) mice.

    Journal: BMC Developmental Biology

    Article Title: Differential requirement for Dab2 in the development of embryonic and extra-embryonic tissues

    doi: 10.1186/1471-213X-13-39

    Figure Lengend Snippet: Conditional dab2 deletion by Meox2-Cre and Sox2-Cre transgene. Female dab2 (fl/fl) mice were crossed with male dab2 (df/+);Meox2-Cre or dab2 (df/+);Sox2-Cre mice to produce mosaic mutant mice without deletion in the extraembryonic endoderm. Mice of around the age of 3 months were used for these analyses. (A) An example of PCR genotyping assay using tail DNA of the progenies (#1 to 12) generated from the cross between dab2 (fl/fl) and dab2 (df/+);Meox2-Cre. Specific PCR amplifications to identify the locus for cre, fl, df, and wt were performed either separately or in a combined reaction. Examples of Dab2 immunostainings in kidney of a dab2 (df/fl) (B) and a dab2 (df/fl);Meox2-Cre (C) mouse are shown, with corresponding PCR genotyping results shown at the upper right corner. (D) An example of PCR genotyping assay using tail DNA of the progenies (#71 to 94) generated from crosses between dab2 (fl/fl) and dab2 (df/+);Sox2-Cre. (E) DNA gel resolving the genotyping PCR products shows a comparison of the deletion efficiency generated by Sox2-Cre or Meox2-Cre. Corresponding Dab2 stainings show the extent of Dab2 mosaicism in the kidney sections from dab2 (+/df) (F) , dab2 (fl/df);Sox2-Cre (G) , and dab2 (fl/df);Meox2-Cre (H) mice.

    Article Snippet: Construction of the dab2 homologous recombination targeting vector and conditional mutant mice Using the mouse Dab2 cDNA as a probe, three clones (λ20, λ24, and λ28) of mouse genomic DNA containing the dab2 gene were isolated from a 129/Sv genome library in λZap (Stratagene) [ ].

    Techniques: Mouse Assay, Mutagenesis, Polymerase Chain Reaction, Genotyping Assay, Generated

    Efficiency of dab2 deletion in embryonic and extraembryonic tissues by Sox2-Cre in E9.5 embryos. E9.5 embryos from a timed mating between female dab2 (fl/fl) and male dab2 (df/+);Sox2-Cre were dissected and harvested for analysis. (A) The example shows the region dissected as embryo (#1) and extraembryonic tissue (#2). (B) The embryonic (e) and extraembryonic (ex) tissues were separated and used for PCR genotyping of dab2 gene to determine the presence of Cre (400 bp), df (529 bp), fl (284 bp), and wildtype (wt, 180 bp) allele, to estimate the efficiency of dab2 gene deletion.

    Journal: BMC Developmental Biology

    Article Title: Differential requirement for Dab2 in the development of embryonic and extra-embryonic tissues

    doi: 10.1186/1471-213X-13-39

    Figure Lengend Snippet: Efficiency of dab2 deletion in embryonic and extraembryonic tissues by Sox2-Cre in E9.5 embryos. E9.5 embryos from a timed mating between female dab2 (fl/fl) and male dab2 (df/+);Sox2-Cre were dissected and harvested for analysis. (A) The example shows the region dissected as embryo (#1) and extraembryonic tissue (#2). (B) The embryonic (e) and extraembryonic (ex) tissues were separated and used for PCR genotyping of dab2 gene to determine the presence of Cre (400 bp), df (529 bp), fl (284 bp), and wildtype (wt, 180 bp) allele, to estimate the efficiency of dab2 gene deletion.

    Article Snippet: Construction of the dab2 homologous recombination targeting vector and conditional mutant mice Using the mouse Dab2 cDNA as a probe, three clones (λ20, λ24, and λ28) of mouse genomic DNA containing the dab2 gene were isolated from a 129/Sv genome library in λZap (Stratagene) [ ].

    Techniques: Polymerase Chain Reaction

    Endoderm disorganization of Dab2 knockout embryoid bodies. Clones of ES cells isolated from blastocysts derived from matings between dab2 (+/df) parents were genotyped by PCR and allowed to form embryoid bodies in suspension cultures for 5 days. Representative examples of heterozygous dab2 (+/-) and homozygous dab2 (-/-) embryoid bodies were analyzed by immunofluorescence microscopy. (A) Sections were stained for the presence of Dab2 (green), endoderm marker GATA4 (red), and counterstained with DAPI (blue). Merged images at low (top panels) and higher magnification (lower panels) are shown. (B) Sections were stained for the pluripotent marker Oct3/4 (green), laminin (red) to indicate primitive endoderm epithelia, and counter-stained with DAPI (blue). Merged images are shown at the top (low magnification) and middle (higher magnification) panels. Laminin staining (red) alone from the corresponding middle panels is presented at the bottom, and the presence of a thin basement membrane underlying the endoderm epithelium in the wildtype embryoid bodies is indicated by an arrow, and no such basement membrane layer was observed in the dab2 null embryoid bodies.

    Journal: BMC Developmental Biology

    Article Title: Differential requirement for Dab2 in the development of embryonic and extra-embryonic tissues

    doi: 10.1186/1471-213X-13-39

    Figure Lengend Snippet: Endoderm disorganization of Dab2 knockout embryoid bodies. Clones of ES cells isolated from blastocysts derived from matings between dab2 (+/df) parents were genotyped by PCR and allowed to form embryoid bodies in suspension cultures for 5 days. Representative examples of heterozygous dab2 (+/-) and homozygous dab2 (-/-) embryoid bodies were analyzed by immunofluorescence microscopy. (A) Sections were stained for the presence of Dab2 (green), endoderm marker GATA4 (red), and counterstained with DAPI (blue). Merged images at low (top panels) and higher magnification (lower panels) are shown. (B) Sections were stained for the pluripotent marker Oct3/4 (green), laminin (red) to indicate primitive endoderm epithelia, and counter-stained with DAPI (blue). Merged images are shown at the top (low magnification) and middle (higher magnification) panels. Laminin staining (red) alone from the corresponding middle panels is presented at the bottom, and the presence of a thin basement membrane underlying the endoderm epithelium in the wildtype embryoid bodies is indicated by an arrow, and no such basement membrane layer was observed in the dab2 null embryoid bodies.

    Article Snippet: Construction of the dab2 homologous recombination targeting vector and conditional mutant mice Using the mouse Dab2 cDNA as a probe, three clones (λ20, λ24, and λ28) of mouse genomic DNA containing the dab2 gene were isolated from a 129/Sv genome library in λZap (Stratagene) [ ].

    Techniques: Knock-Out, Clone Assay, Isolation, Derivative Assay, Polymerase Chain Reaction, Immunofluorescence, Microscopy, Staining, Marker

    Conditional targeting strategy and screening for homologous recombination of dab2 gene. (A) Schematic illustration of dab2 gene targeting strategy and gene deletion in mutant mice is shown. The targeting construct was made by inserting a neomycin resistance gene (Neo-R) flanked by Frt sites (closed triangles) between exon 4 and 5. LoxP sites (open triangles) were placed flanking exon 3 and 4. Correct homologous recombination of the targeting construct did not alter dab2 gene but allowed expression of Neo-R for selection of mutant ES clones. Following selection and verification, chimeric and then germline mutant mice were made from the mutant ES cells. The Neo-R cassette was excised by crossing with Flp expression mice to generate the floxed allele. The deletion of exon 4 removed a potential alternative translation start site, indicated as “ATG”. (B) Examples of PCR screening assay of neomycin resistant clones (#25-30) following transfection of linearized targeting construct and drug selection. Each clone was amplified separately for wildtype (886 bp) and recombinant allele (1714 bp, as predicted). Here, clone #28 was identified as positive. In the agarose gel: lane 1: Mw markers; lane 2: control mouse DNA; lane 3: KO construct; lane 4: control WT mix, no DNA; 5: control mix for mutant, no DNA; lanes 6 to 17, amplification using DNA template from clone #25 to 30. (C) Examples of targeted allele in selected clones (#1, #28, and #121). Here, clone #1 was found to lack the loxP1 site, whereas clones #28 and #121 contained all components: LoxP1, 1714 bp; LoxP2, 795 bp; Frt1, 635 bp; Frt2, 837 bp; and Neo, 617 bp.

    Journal: BMC Developmental Biology

    Article Title: Differential requirement for Dab2 in the development of embryonic and extra-embryonic tissues

    doi: 10.1186/1471-213X-13-39

    Figure Lengend Snippet: Conditional targeting strategy and screening for homologous recombination of dab2 gene. (A) Schematic illustration of dab2 gene targeting strategy and gene deletion in mutant mice is shown. The targeting construct was made by inserting a neomycin resistance gene (Neo-R) flanked by Frt sites (closed triangles) between exon 4 and 5. LoxP sites (open triangles) were placed flanking exon 3 and 4. Correct homologous recombination of the targeting construct did not alter dab2 gene but allowed expression of Neo-R for selection of mutant ES clones. Following selection and verification, chimeric and then germline mutant mice were made from the mutant ES cells. The Neo-R cassette was excised by crossing with Flp expression mice to generate the floxed allele. The deletion of exon 4 removed a potential alternative translation start site, indicated as “ATG”. (B) Examples of PCR screening assay of neomycin resistant clones (#25-30) following transfection of linearized targeting construct and drug selection. Each clone was amplified separately for wildtype (886 bp) and recombinant allele (1714 bp, as predicted). Here, clone #28 was identified as positive. In the agarose gel: lane 1: Mw markers; lane 2: control mouse DNA; lane 3: KO construct; lane 4: control WT mix, no DNA; 5: control mix for mutant, no DNA; lanes 6 to 17, amplification using DNA template from clone #25 to 30. (C) Examples of targeted allele in selected clones (#1, #28, and #121). Here, clone #1 was found to lack the loxP1 site, whereas clones #28 and #121 contained all components: LoxP1, 1714 bp; LoxP2, 795 bp; Frt1, 635 bp; Frt2, 837 bp; and Neo, 617 bp.

    Article Snippet: Construction of the dab2 homologous recombination targeting vector and conditional mutant mice Using the mouse Dab2 cDNA as a probe, three clones (λ20, λ24, and λ28) of mouse genomic DNA containing the dab2 gene were isolated from a 129/Sv genome library in λZap (Stratagene) [ ].

    Techniques: Homologous Recombination, Mutagenesis, Mouse Assay, Construct, Expressing, Selection, Clone Assay, Polymerase Chain Reaction, Screening Assay, Transfection, Amplification, Recombinant, Agarose Gel Electrophoresis