homogenization buffer  (Roche)


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    Structured Review

    Roche homogenization buffer
    Homogenization Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/homogenization buffer/product/Roche
    Average 94 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    homogenization buffer - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Homogenization:

    Article Title: Exercise Modulates Chloride Homeostasis after Spinal Cord Injury
    Article Snippet: .. Blocks of spinal tissue (L4–L6) were immersed in ice-cold homogenization buffer (modified RIPA; 50 m m Tris buffer, pH 6.8, 1% Triton X-100, 0.1% SDS, 1 m m DTT, 0.5% deoxycholate, 150 m m NaCl) containing a mixture of protease and phosphatase inhibitors (Roche), 2 m m phenyl-methyl sulfonyl-fluoride, and 1 m m sodium fluoride. .. An equal amount of total protein was loaded in every well and separated on 10–16% SDS-PAGE gels (BDNF and TrkB) or 4–20% Mini Protean TGX gels (Bio-Rad) (KCC2 and NKCC1).

    Article Title: Warming Induces Significant Reprogramming of Beige, But Not Brown, Adipocyte Cellular Identity
    Article Snippet: .. Pieces of frozen adipose tissue (50–100mg) were homogenized by dounce (7ml) in homogenization buffer (50mM Tris [pH7.5], 12mM MgCl2 , 100mM KCl, 1% NP-40; 100μg/ml cycloheximide, 1mg/ml sodium heparin, 2mM DTT, 0.2units/μl RNasin, 1x Roche Complete EDTA-free protease inhibitor); 1ml and 4ml for BAT and iWAT, respectively) and incubated on ice for 15 min with vortexing every 5 min. Lysates were centrifuged at 13,000rpm for 10min and the supernatant was collected after removing the top lipid layer. .. Anti-GFP antibody (5μg/ml, Abcam ab290) was added to the collected supernatant and rotated for 1h at 4°C.

    Article Title: Structural basis of keto acid utilization in nonribosomal depsipeptide synthesis.
    Article Snippet: .. Nonribosomal depsipeptides are natural products composed of amino and hydroxy acid residues. .. Nonribosomal depsipeptides are natural products composed of amino and hydroxy acid residues.

    Article Title: Maternal Pharmacokinetics and Fetal Disposition of (±)-Citalopram during Mouse Pregnancy
    Article Snippet: .. Microsomes were prepared by mincing and cleaning tissues in wash buffer to remove blood (225 mM mannitol, 75 mM sucrose, 30 mM Tris-HCl, pH 7.4) and isolated in homogenization buffer (2 mL per 1 g of tissue) with a Teflon pestle (wash buffer + 0.5% (wt/vol) BSA + 0.1 mM EDTA + Roche complete protease inhibitor (Roche; #04693116001, 1 tablet/10 mL). .. The crude plasma membrane fraction of the homogenate was obtained by two centrifugation steps at 800 g for 5 min (discarding the pellet each time) and an additional centrifugation at 10 000 g for 10 min. Microsomal and cytosolic proteins were isolated by centrifugation of the supernatant at 25 000 g for 20 min followed by 95 000 g for 2.5 h. Microsomal proteins present in the pellet fraction were resuspended in wash buffer and centrifuged at 95 000 g for 2.5 h. The pellet containing microsomal proteins was resuspended in 1 mL of wash buffer with protease inhibitor.

    Article Title: Dynamic in Vivo Binding of STAT5 to Growth Hormone-Regulated Genes in Intact Rat Liver. Sex-Specific Binding at Low- But Not High-Affinity STAT5 Sites
    Article Snippet: .. Liver homogenates were prepared from frozen rat liver tissues as described ( ) by homogenizing pieces of liver on ice in homogenization buffer [10 m m Tris-Cl (pH 7.6), 1 m m EDTA, 250 m m sucrose] containing complete protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail (Roche Diagnostics, Indianapolis, IN). .. The homogenates were centrifuged for 20 min at 9000 rpm at 4 C in a microfuge, and the supernatants were stored at −80 C. Liver homogenates were used in all EMSA experiments except for the determination of dissociation constants (see below), where nuclear extracts from transfected and GH-stimulated 293T cells were used.

    Article Title: Sexually dimorphic responses to MPTP found in microglia, inflammation and gut microbiota in a progressive monkey model of Parkinson’s disease
    Article Snippet: .. Biopsies were later processed with homogenization buffer including a protease inhibitor (Roche) and 1% Triton X-100 and homogenized in a TissueLyser II (Qiagen), centrifuged at 20,000 rcf at 4°C for 10 minutes after which the supernatant was collected for multiplexed immunoassay analyses. ..

    Article Title: Matrix‐glycoprotein interactions required for budding of a plant nucleorhabdovirus and induction of inner nuclear membrane invagination
    Article Snippet: .. Leaf samples (0.5 g) from Agrobacterium ‐infiltrations were collected at 2 dpi, gently ground into a fine powder with a mortar and pestle in liquid nitrogen, followed by extraction in three volumes of homogenization buffer [50 mM Tris–HCl, pH 8.0, 10 mM KCl, 3 mM MgCl2 , 1 mM EDTA, 1 mM DTT, 0.1% BSA, 0.3% dextran, 13% (w/v) sucrose, and Complete Mini Protease Inhibitor Cocktail (Roche, Germany)]. .. 300 μL supernatants were collected and transferred to clean centrifuge tubes and then mixed with 1.6 mL of 85% sucrose to a final concentration of 71.5% sucrose.

    Article Title: Aging increases vulnerability to stress-induced depression via upregulation of NADPH oxidase in mice
    Article Snippet: .. Hippocampal tissue or cultured cells were homogenized in a homogenization buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% Nonidet P-40, 0.1% SDS, and 0.1% sodium deoxycholate) containing a cocktail of protease inhibitors (Roche, Mannheim, Germany) by sonication on ice using an Epishear probe sonicator (Active Motif, Carlsbad, CA, USA) at a 40% power outlet with two rounds of 10-s pulses. .. Protein concentration was determined by the Bradford method (Bio-Rad Laboratories.

    Protease Inhibitor:

    Article Title: Warming Induces Significant Reprogramming of Beige, But Not Brown, Adipocyte Cellular Identity
    Article Snippet: .. Pieces of frozen adipose tissue (50–100mg) were homogenized by dounce (7ml) in homogenization buffer (50mM Tris [pH7.5], 12mM MgCl2 , 100mM KCl, 1% NP-40; 100μg/ml cycloheximide, 1mg/ml sodium heparin, 2mM DTT, 0.2units/μl RNasin, 1x Roche Complete EDTA-free protease inhibitor); 1ml and 4ml for BAT and iWAT, respectively) and incubated on ice for 15 min with vortexing every 5 min. Lysates were centrifuged at 13,000rpm for 10min and the supernatant was collected after removing the top lipid layer. .. Anti-GFP antibody (5μg/ml, Abcam ab290) was added to the collected supernatant and rotated for 1h at 4°C.

    Article Title: Structural basis of keto acid utilization in nonribosomal depsipeptide synthesis.
    Article Snippet: .. Nonribosomal depsipeptides are natural products composed of amino and hydroxy acid residues. .. Nonribosomal depsipeptides are natural products composed of amino and hydroxy acid residues.

    Article Title: Maternal Pharmacokinetics and Fetal Disposition of (±)-Citalopram during Mouse Pregnancy
    Article Snippet: .. Microsomes were prepared by mincing and cleaning tissues in wash buffer to remove blood (225 mM mannitol, 75 mM sucrose, 30 mM Tris-HCl, pH 7.4) and isolated in homogenization buffer (2 mL per 1 g of tissue) with a Teflon pestle (wash buffer + 0.5% (wt/vol) BSA + 0.1 mM EDTA + Roche complete protease inhibitor (Roche; #04693116001, 1 tablet/10 mL). .. The crude plasma membrane fraction of the homogenate was obtained by two centrifugation steps at 800 g for 5 min (discarding the pellet each time) and an additional centrifugation at 10 000 g for 10 min. Microsomal and cytosolic proteins were isolated by centrifugation of the supernatant at 25 000 g for 20 min followed by 95 000 g for 2.5 h. Microsomal proteins present in the pellet fraction were resuspended in wash buffer and centrifuged at 95 000 g for 2.5 h. The pellet containing microsomal proteins was resuspended in 1 mL of wash buffer with protease inhibitor.

    Article Title: Dynamic in Vivo Binding of STAT5 to Growth Hormone-Regulated Genes in Intact Rat Liver. Sex-Specific Binding at Low- But Not High-Affinity STAT5 Sites
    Article Snippet: .. Liver homogenates were prepared from frozen rat liver tissues as described ( ) by homogenizing pieces of liver on ice in homogenization buffer [10 m m Tris-Cl (pH 7.6), 1 m m EDTA, 250 m m sucrose] containing complete protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail (Roche Diagnostics, Indianapolis, IN). .. The homogenates were centrifuged for 20 min at 9000 rpm at 4 C in a microfuge, and the supernatants were stored at −80 C. Liver homogenates were used in all EMSA experiments except for the determination of dissociation constants (see below), where nuclear extracts from transfected and GH-stimulated 293T cells were used.

    Article Title: Sexually dimorphic responses to MPTP found in microglia, inflammation and gut microbiota in a progressive monkey model of Parkinson’s disease
    Article Snippet: .. Biopsies were later processed with homogenization buffer including a protease inhibitor (Roche) and 1% Triton X-100 and homogenized in a TissueLyser II (Qiagen), centrifuged at 20,000 rcf at 4°C for 10 minutes after which the supernatant was collected for multiplexed immunoassay analyses. ..

    Article Title: Matrix‐glycoprotein interactions required for budding of a plant nucleorhabdovirus and induction of inner nuclear membrane invagination
    Article Snippet: .. Leaf samples (0.5 g) from Agrobacterium ‐infiltrations were collected at 2 dpi, gently ground into a fine powder with a mortar and pestle in liquid nitrogen, followed by extraction in three volumes of homogenization buffer [50 mM Tris–HCl, pH 8.0, 10 mM KCl, 3 mM MgCl2 , 1 mM EDTA, 1 mM DTT, 0.1% BSA, 0.3% dextran, 13% (w/v) sucrose, and Complete Mini Protease Inhibitor Cocktail (Roche, Germany)]. .. 300 μL supernatants were collected and transferred to clean centrifuge tubes and then mixed with 1.6 mL of 85% sucrose to a final concentration of 71.5% sucrose.

    Cell Culture:

    Article Title: Aging increases vulnerability to stress-induced depression via upregulation of NADPH oxidase in mice
    Article Snippet: .. Hippocampal tissue or cultured cells were homogenized in a homogenization buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% Nonidet P-40, 0.1% SDS, and 0.1% sodium deoxycholate) containing a cocktail of protease inhibitors (Roche, Mannheim, Germany) by sonication on ice using an Epishear probe sonicator (Active Motif, Carlsbad, CA, USA) at a 40% power outlet with two rounds of 10-s pulses. .. Protein concentration was determined by the Bradford method (Bio-Rad Laboratories.

    Protein Purification:

    Article Title: Structural basis of keto acid utilization in nonribosomal depsipeptide synthesis.
    Article Snippet: .. Nonribosomal depsipeptides are natural products composed of amino and hydroxy acid residues. .. Nonribosomal depsipeptides are natural products composed of amino and hydroxy acid residues.

    Incubation:

    Article Title: Warming Induces Significant Reprogramming of Beige, But Not Brown, Adipocyte Cellular Identity
    Article Snippet: .. Pieces of frozen adipose tissue (50–100mg) were homogenized by dounce (7ml) in homogenization buffer (50mM Tris [pH7.5], 12mM MgCl2 , 100mM KCl, 1% NP-40; 100μg/ml cycloheximide, 1mg/ml sodium heparin, 2mM DTT, 0.2units/μl RNasin, 1x Roche Complete EDTA-free protease inhibitor); 1ml and 4ml for BAT and iWAT, respectively) and incubated on ice for 15 min with vortexing every 5 min. Lysates were centrifuged at 13,000rpm for 10min and the supernatant was collected after removing the top lipid layer. .. Anti-GFP antibody (5μg/ml, Abcam ab290) was added to the collected supernatant and rotated for 1h at 4°C.

    Isolation:

    Article Title: Maternal Pharmacokinetics and Fetal Disposition of (±)-Citalopram during Mouse Pregnancy
    Article Snippet: .. Microsomes were prepared by mincing and cleaning tissues in wash buffer to remove blood (225 mM mannitol, 75 mM sucrose, 30 mM Tris-HCl, pH 7.4) and isolated in homogenization buffer (2 mL per 1 g of tissue) with a Teflon pestle (wash buffer + 0.5% (wt/vol) BSA + 0.1 mM EDTA + Roche complete protease inhibitor (Roche; #04693116001, 1 tablet/10 mL). .. The crude plasma membrane fraction of the homogenate was obtained by two centrifugation steps at 800 g for 5 min (discarding the pellet each time) and an additional centrifugation at 10 000 g for 10 min. Microsomal and cytosolic proteins were isolated by centrifugation of the supernatant at 25 000 g for 20 min followed by 95 000 g for 2.5 h. Microsomal proteins present in the pellet fraction were resuspended in wash buffer and centrifuged at 95 000 g for 2.5 h. The pellet containing microsomal proteins was resuspended in 1 mL of wash buffer with protease inhibitor.

    Modification:

    Article Title: Exercise Modulates Chloride Homeostasis after Spinal Cord Injury
    Article Snippet: .. Blocks of spinal tissue (L4–L6) were immersed in ice-cold homogenization buffer (modified RIPA; 50 m m Tris buffer, pH 6.8, 1% Triton X-100, 0.1% SDS, 1 m m DTT, 0.5% deoxycholate, 150 m m NaCl) containing a mixture of protease and phosphatase inhibitors (Roche), 2 m m phenyl-methyl sulfonyl-fluoride, and 1 m m sodium fluoride. .. An equal amount of total protein was loaded in every well and separated on 10–16% SDS-PAGE gels (BDNF and TrkB) or 4–20% Mini Protean TGX gels (Bio-Rad) (KCC2 and NKCC1).

    Sonication:

    Article Title: Structural basis of keto acid utilization in nonribosomal depsipeptide synthesis.
    Article Snippet: .. Nonribosomal depsipeptides are natural products composed of amino and hydroxy acid residues. .. Nonribosomal depsipeptides are natural products composed of amino and hydroxy acid residues.

    Article Title: Aging increases vulnerability to stress-induced depression via upregulation of NADPH oxidase in mice
    Article Snippet: .. Hippocampal tissue or cultured cells were homogenized in a homogenization buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% Nonidet P-40, 0.1% SDS, and 0.1% sodium deoxycholate) containing a cocktail of protease inhibitors (Roche, Mannheim, Germany) by sonication on ice using an Epishear probe sonicator (Active Motif, Carlsbad, CA, USA) at a 40% power outlet with two rounds of 10-s pulses. .. Protein concentration was determined by the Bradford method (Bio-Rad Laboratories.

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  • 85
    Roche nt 3 elisa homogenization buffer
    Graphs of <t>NT-3</t> concentration measured by <t>ELISA</t> in several sites as a function of time after a single AAV(NT-3) administration into the left MG muscle. Each point represents values from a single treated preparation. A) NT-3 concentration in injected MG
    Nt 3 Elisa Homogenization Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nt 3 elisa homogenization buffer/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    85
    Roche detergent free tissue homogenisation buffer
    Tau phosphorylation is reduced in HFD fed APOE mice, independent of genotype. The frontal cortex from APOE mice were homogenised in sucrose <t>homogenisation</t> buffer, lysates were then immunoblotted with the indicated antibodies. A) Western blot of tau antibodies in frontal cortex of APOE KO, APOEε3, APOEε4 and WT mice fed LFD or HFD. B) Density of phosphorylated tau normalised against total tau. C) Tau phosphorylation is dependent on diet. Phosphorylated tau is normalised to total tau, data is grouped by diet. *p
    Detergent Free Tissue Homogenisation Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/detergent free tissue homogenisation buffer/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    detergent free tissue homogenisation buffer - by Bioz Stars, 2020-09
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    88
    Roche tbs homogenization buffer
    Toll-like receptor 2 protein is increased in Parkinson’s disease brain. a Immunoblot analysis of TLR2 from different regions of healthy brain following sequential extraction of proteins in <t>tris-buffered</t> saline <t>(TBS</t> buffer) followed by SDS buffer. ACC anterior cingulate cortex, PUT putamen, CER cerebellum, WM white matter, OCC occipital cortex, SN substantia nigra. Human peripheral blood mononuclear cells (PBMCs) were used as a positive control for TLR2 expression. β-Actin was used as a loading control. Immunoblot images are representative of n = 3 cases. b Quantification of relative TLR2 expression in different human brain regions after normalization to β-actin. Data are mean ± SEM, n = 3. c Representative immunoblot images from control and Parkinson’s disease (PD) anterior cingulate cortex. Quantification of TLR2 (d) and total α-synuclein (e) protein in control and PD brain tissue after normalization to β-actin. Data are mean ± SEM, n = 10 controls and 17 PD cases. Multivariate analysis was used to determine a significant difference at p
    Tbs Homogenization Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tbs homogenization buffer/product/Roche
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
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    85
    Roche zymography homogenization buffer
    Activity of matrix metalloproteinase-9 determined by gelatin in vitro <t>zymography.</t> Values represent MMP-9 semi-quantitatively. Activities between groups differed significantly ( P = .0003). Control and sham did not differ statistically significant
    Zymography Homogenization Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zymography homogenization buffer/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zymography homogenization buffer - by Bioz Stars, 2020-09
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    Image Search Results


    Graphs of NT-3 concentration measured by ELISA in several sites as a function of time after a single AAV(NT-3) administration into the left MG muscle. Each point represents values from a single treated preparation. A) NT-3 concentration in injected MG

    Journal: The European journal of neuroscience

    Article Title: Intramuscular AAV delivery of NT-3 alters synaptic transmission to motoneurons in adult rats

    doi: 10.1111/j.1460-9568.2010.07392.x

    Figure Lengend Snippet: Graphs of NT-3 concentration measured by ELISA in several sites as a function of time after a single AAV(NT-3) administration into the left MG muscle. Each point represents values from a single treated preparation. A) NT-3 concentration in injected MG

    Article Snippet: Tissue samples were homogenized in NT-3 ELISA homogenization buffer (137mM NaCl, 20mM Tris-HCl (pH 8.0), 1% Triton X-100, 10% glycerol, and protease inhibitor cocktail tablet (Roche, IN) as follows: pooled DRGs or spinal cord samples each at the L4/L5 segmental level were homogenized in 250 μl or 500 μl buffer respectively with a pellet pestle on ice before sonication for 45 seconds.

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Injection

    Tau phosphorylation is reduced in HFD fed APOE mice, independent of genotype. The frontal cortex from APOE mice were homogenised in sucrose homogenisation buffer, lysates were then immunoblotted with the indicated antibodies. A) Western blot of tau antibodies in frontal cortex of APOE KO, APOEε3, APOEε4 and WT mice fed LFD or HFD. B) Density of phosphorylated tau normalised against total tau. C) Tau phosphorylation is dependent on diet. Phosphorylated tau is normalised to total tau, data is grouped by diet. *p

    Journal: PLoS ONE

    Article Title: The ?3 and ?4 Alleles of Human APOE Differentially Affect Tau Phosphorylation in Hyperinsulinemic and Pioglitazone Treated Mice

    doi: 10.1371/journal.pone.0016991

    Figure Lengend Snippet: Tau phosphorylation is reduced in HFD fed APOE mice, independent of genotype. The frontal cortex from APOE mice were homogenised in sucrose homogenisation buffer, lysates were then immunoblotted with the indicated antibodies. A) Western blot of tau antibodies in frontal cortex of APOE KO, APOEε3, APOEε4 and WT mice fed LFD or HFD. B) Density of phosphorylated tau normalised against total tau. C) Tau phosphorylation is dependent on diet. Phosphorylated tau is normalised to total tau, data is grouped by diet. *p

    Article Snippet: Protein extraction The frontal cortex region was weighed whilst frozen and then homogenised rapidly in 10 fold volume of a detergent free tissue homogenisation buffer (250 mM sucrose, 20 mM Tris-HCl (pH 7.4), 1 mM EGTA, 1 mM EGTA, 100 mM PMSF, 1 mini complete protease inhibitor cocktail tablet (Roche, UK) and 1 mini complete phosphatase inhibitor cocktail tablet (Roche, UK)) on ice.

    Techniques: Mouse Assay, Homogenization, Western Blot

    Toll-like receptor 2 protein is increased in Parkinson’s disease brain. a Immunoblot analysis of TLR2 from different regions of healthy brain following sequential extraction of proteins in tris-buffered saline (TBS buffer) followed by SDS buffer. ACC anterior cingulate cortex, PUT putamen, CER cerebellum, WM white matter, OCC occipital cortex, SN substantia nigra. Human peripheral blood mononuclear cells (PBMCs) were used as a positive control for TLR2 expression. β-Actin was used as a loading control. Immunoblot images are representative of n = 3 cases. b Quantification of relative TLR2 expression in different human brain regions after normalization to β-actin. Data are mean ± SEM, n = 3. c Representative immunoblot images from control and Parkinson’s disease (PD) anterior cingulate cortex. Quantification of TLR2 (d) and total α-synuclein (e) protein in control and PD brain tissue after normalization to β-actin. Data are mean ± SEM, n = 10 controls and 17 PD cases. Multivariate analysis was used to determine a significant difference at p

    Journal: Acta Neuropathologica

    Article Title: Toll-like receptor 2 is increased in neurons in Parkinson’s disease brain and may contribute to alpha-synuclein pathology

    doi: 10.1007/s00401-016-1648-8

    Figure Lengend Snippet: Toll-like receptor 2 protein is increased in Parkinson’s disease brain. a Immunoblot analysis of TLR2 from different regions of healthy brain following sequential extraction of proteins in tris-buffered saline (TBS buffer) followed by SDS buffer. ACC anterior cingulate cortex, PUT putamen, CER cerebellum, WM white matter, OCC occipital cortex, SN substantia nigra. Human peripheral blood mononuclear cells (PBMCs) were used as a positive control for TLR2 expression. β-Actin was used as a loading control. Immunoblot images are representative of n = 3 cases. b Quantification of relative TLR2 expression in different human brain regions after normalization to β-actin. Data are mean ± SEM, n = 3. c Representative immunoblot images from control and Parkinson’s disease (PD) anterior cingulate cortex. Quantification of TLR2 (d) and total α-synuclein (e) protein in control and PD brain tissue after normalization to β-actin. Data are mean ± SEM, n = 10 controls and 17 PD cases. Multivariate analysis was used to determine a significant difference at p

    Article Snippet: Briefly, tissue was mechanically homogenized in ten volumes of TBS homogenization buffer (50 mM Tris, 125 mM NaCl, pH 7.4, 5 mM EDTA, 0.02% sodium azide) containing protease inhibitor cocktail (Roche), followed by centrifugation at 100,000×g for 2 h at 4 °C, with supernatant collected as the TBS-soluble fraction containing cytosolic proteins.

    Techniques: Positive Control, Expressing

    Activity of matrix metalloproteinase-9 determined by gelatin in vitro zymography. Values represent MMP-9 semi-quantitatively. Activities between groups differed significantly ( P = .0003). Control and sham did not differ statistically significant

    Journal: European Journal of Cardio-Thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery

    Article Title: Glutathione preconditioning ameliorates mitochondria dysfunction during warm pulmonary ischemia-reperfusion injury

    doi: 10.1016/j.ejcts.2011.02.081

    Figure Lengend Snippet: Activity of matrix metalloproteinase-9 determined by gelatin in vitro zymography. Values represent MMP-9 semi-quantitatively. Activities between groups differed significantly ( P = .0003). Control and sham did not differ statistically significant

    Article Snippet: Zymography homogenization buffer (Buffer 6) contained 25 mM Tris/HCl, 150 mM NaCl, 1% Na-deoxycholate, 1% NP 40%, 0.1% Na-dodecylsulfate (SDS), and Complete Protease Inhibitor Cocktail Set, EDTA-free (Roche Diagnostics Grenzach, Germany) at pH 7.6.

    Techniques: Activity Assay, In Vitro, Zymography