home‐made mirna target prediction software (Arraystar inc)
90
Structured Review
Arraystar inc
home‐made mirna target prediction software
Home‐Made Mirna Target Prediction Software, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/home-made+software/mirna+target+prediction+software/pmc10323880-151-10-15
Average 90 stars, based on 1 article reviews
Home‐Made Mirna Target Prediction Software, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/home-made+software/mirna+target+prediction+software/pmc10323880-151-10-15
Average 90 stars, based on 1 article reviews
home‐made mirna target prediction software - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "Circular RNA circ‐TNRC6B inhibits the proliferation and invasion of esophageal squamous cell carcinoma cells by regulating the miR ‐452‐5p/ DAG1 axis"
Article Title: Circular RNA circ‐TNRC6B inhibits the proliferation and invasion of esophageal squamous cell carcinoma cells by regulating the miR ‐452‐5p/ DAG1 axis
Journal: Molecular Oncology
doi: 10.1002/1878-0261.13432
Figure Legend Snippet: circ‐TNRC6B serves as a miR‐452‐5p sponge in ESCC cells. (A) CircRNA‐miRNA binding circle diagram showing potential miRNA targets of circ‐TNRC6B predicted by Arraystar's home‐made miRNA target prediction software based on TargetScan and miRanda. (B) Venn diagram showing the intersection of potential miRNA targets of circ‐TNRC6B based on Arraystar's home‐made miRNA target prediction software, ENCORI, and circBank databases. (C) The complementary sequences of circ‐TNRC6B and miR‐452‐5p were predicted by Arraystar's home‐made miRNA target prediction software. (D) The MFE secondary structure of circ‐TNRC6B predicted by RNAfold web server and the part where the secondary structure of circ‐TNRC6B is relevant for the binding of miR‐452‐5p. (E) FISH assay indicated circ‐TNRC6B and miR‐452‐5p were co‐localized in the cytoplasm of KYSE150 cells ( n = 3). Scale bar: 20 μm. (F) RIP analysis in KYSE150 cells co‐transfected with Myc‐AGO2 vector and miR‐452‐5p mimics. Data are represented as mean ± SD ( n = 3, P ‐values were determined by Mann–Whitney U ‐test). (G) RIP analysis in KYSE150 cells co‐transfected with Myc‐AGO2 vector and NC ( n = 2, P ‐values were determined by Mann–Whitney U ‐test). Data are represented as mean ± SD. (H) Dual‐luciferase reporter gene analysis detected the luciferase activity of circ‐TNRC6B when co‐transfected with luc‐empty vector or wild‐type luc‐circ‐TNRC6B vector and NC or miR‐452‐5p mimics in KYSE150 cells. Data are represented as mean ± SD ( n = 3, P ‐values were determined by Mann–Whitney U ‐test). (I) Dual‐luciferase reporter gene analysis detected the luciferase activity of circ‐TNRC6B when co‐transfected with mutant luc‐circ‐TNRC6B vector and NC or miR‐452‐5p mimics in KYSE150 cells. Data are represented as mean ± SD ( n = 3, P ‐values were determined by Mann–Whitney U ‐test). (J) TE1 cells were transfected with pLC5 empty vector or circ‐TNRC6B vector; then, the relative expression of circ‐TNRC6B and miR‐452‐5p were detected by qRT‐PCR assay. Data are represented as mean ± SD ( n = 2, P ‐values were determined by Mann–Whitney U ‐test). (K) KYSE150 cells were transfected with si‐NC or si‐circ‐TNRC6B; then, the relative expression of circ‐TNRC6B and miR‐452‐5p was detected by qRT‐PCR assay. Data are represented as mean ± SD ( n = 2, P ‐values were determined by Mann–Whitney U ‐test). * P < 0.05, ** P < 0.01.
Techniques Used: Binding Assay, Software, Transfection, Plasmid Preparation, MANN-WHITNEY, Luciferase, Activity Assay, Mutagenesis, Expressing, Quantitative RT-PCR
Figure Legend Snippet: miR‐452‐5p promotes the proliferation, migration, and invasion abilities of ESCC cells. (A) The relative expression level of miR‐452‐5p in four ESCC cell lines was examined by qRT‐PCR ( n = 2). Data are represented as mean ± SD. (B, C) The proliferative and clonogenic ability of TE1 cells after exogenous miR‐452‐5p mimics transfection was detected by CCK‐8 ( n = 4, P ‐values were determined by two‐way ANOVA) and colony formation assay ( n = 3, P ‐values were determined by Mann–Whitney U ‐test). NC: negative control of miRNA mimics. Data are represented as mean ± SD. (D, E) The migration ability of TE1 cells after exogenous miR‐452‐5p mimics transfection was detected by wound healing and transwell migration assay. Data are represented as mean ± SD ( n = 3, P ‐values were determined by Mann–Whitney U ‐test). Scale bar: 100 μm. (F) The invasion ability of TE1 cells after exogenous miR‐452‐5p mimics transfection was detected by transwell invasion assay. Data are represented as mean ± SD ( n = 3, P ‐values were determined by Mann–Whitney U ‐test). Scale bar: 100 μm. (G, H) The proliferative and clonogenic ability of KYSE170 cells after miR‐452‐5p inhibitor transfection was detected by CCK‐8 ( n = 4, P ‐values were determined by two‐way ANOVA) and colony formation assay ( n = 3, P ‐values were determined by Mann–Whitney U ‐test). Inhibitor NC: negative control of miRNA inhibitor. Data are represented as mean ± SD. (I, J) The migration ability of KYSE170 cells after miR‐452‐5p inhibitor transfection was detected by wound‐healing and transwell migration assay. Data are represented as mean ± SD ( n = 3, P ‐values were determined by Mann–Whitney U ‐test). Scale bar: 100 μm. (K) The invasion ability of KYSE170 cells after miR‐452‐5p inhibitor transfection was detected by transwell invasion assay. Data are represented as mean ± SD ( n = 3, P ‐values were determined by Mann–Whitney U ‐test). Scale bar: 100 μm. * P < 0.05, ** P < 0.01, *** P < 0.001.
Techniques Used: Migration, Expressing, Quantitative RT-PCR, Transfection, CCK-8 Assay, Colony Assay, MANN-WHITNEY, Negative Control, Transwell Migration Assay, Transwell Invasion Assay
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(a) Article Title: Circular RNA hsa_circ_0003575 regulates oxLDL induced vascular endothelial cells proliferation and angiogenesis. Article Snippet: Circular RNAs (circRNAs) are a novel class of RNAs generated from back-splicing and characterized by covalently closed continuous loops.. Recently, circRNAs have recently shown large regulation on cardiovascular system, including atherosclerosis.. The present study aims to investigate the circRNA expression profile and identify their roles on vascular endothelial cells induced by oxLDL. Article Title: Comprehensive Analysis of circRNA Expression Profiles in Human Brown Adipose Tissue Article Snippet: The target microRNAs of hsa_circ_0006168 ( A ), hsa_circ_0026337 ( B ), hsa_circ_000750 ( C ), and Article Title: Circular RNA hsa_circ_0016788 regulates hepatocellular carcinoma tumorigenesis through miR-486/CDK4 pathway. Article Snippet: To investigate the potential regulatory mechanism of hsa_circ_0016788 on HCC, bioinformatics tools and Article Title: Circular RNA LPAR3 sponges microRNA‐198 to facilitate esophageal cancer migration, invasion, and metastasis Article Snippet: Using Article Title: Circular RNA Profiling Reveals Exosomal circ_0006156 as a Novel Biomarker in Papillary Thyroid Cancer Article Snippet: The Article Title: Exosome circ_0044516 promotes prostate cancer cell proliferation and metastasis as a potential biomarker. Article Snippet: Department of Urology, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China Minimally Invasive Technique and Product Translational Center, Guangzhou Medical University, Guangzhou, China Department of Urology, The First Affiliated Hospital of Sun Yat‐Sen University, Guangzhou, China Department of Biomedical Engineering, Jinan University, Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Guangzhou, China Expressing:Article Title: Circular RNA expression profile in blood according to ischemic stroke etiology Article Snippet: First, interactions between circRNAs and their Article Title: Circular RNA hsa_circ_0016788 regulates hepatocellular carcinoma tumorigenesis through miR-486/CDK4 pathway. Article Snippet: *p < 0.05, **p < 0.01 compared to control group FIGURE 5 miR-486 targeted hsa_circ_0016788. (a) Article Title: Circular RNA hsa_circ_0003575 regulates oxLDL induced vascular endothelial cells proliferation and angiogenesis. Article Snippet: Circular RNAs (circRNAs) are a novel class of RNAs generated from back-splicing and characterized by covalently closed continuous loops.. Recently, circRNAs have recently shown large regulation on cardiovascular system, including atherosclerosis.. The present study aims to investigate the circRNA expression profile and identify their roles on vascular endothelial cells induced by oxLDL. Article Title: Comprehensive Analysis of circRNA Expression Profiles in Human Brown Adipose Tissue Article Snippet: The target microRNAs of hsa_circ_0006168 ( A ), hsa_circ_0026337 ( B ), hsa_circ_000750 ( C ), and Article Title: Circular RNA hsa_circ_0016788 regulates hepatocellular carcinoma tumorigenesis through miR-486/CDK4 pathway. Article Snippet: To investigate the potential regulatory mechanism of hsa_circ_0016788 on HCC, bioinformatics tools and Article Title: Circular RNA LPAR3 sponges microRNA‐198 to facilitate esophageal cancer migration, invasion, and metastasis Article Snippet: Using Article Title: Circular RNA Profiling Reveals Exosomal circ_0006156 as a Novel Biomarker in Papillary Thyroid Cancer Article Snippet: The Article Title: Exosome circ_0044516 promotes prostate cancer cell proliferation and metastasis as a potential biomarker. Article Snippet: Department of Urology, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China Minimally Invasive Technique and Product Translational Center, Guangzhou Medical University, Guangzhou, China Department of Urology, The First Affiliated Hospital of Sun Yat‐Sen University, Guangzhou, China Department of Biomedical Engineering, Jinan University, Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Guangzhou, China Transfection:Article Title: Circular RNA expression profile in blood according to ischemic stroke etiology Article Snippet: First, interactions between circRNAs and their Article Title: Circular RNA hsa_circ_0016788 regulates hepatocellular carcinoma tumorigenesis through miR-486/CDK4 pathway. Article Snippet: *p < 0.05, **p < 0.01 compared to control group FIGURE 5 miR-486 targeted hsa_circ_0016788. (a) Article Title: Circular RNA hsa_circ_0003575 regulates oxLDL induced vascular endothelial cells proliferation and angiogenesis. Article Snippet: Circular RNAs (circRNAs) are a novel class of RNAs generated from back-splicing and characterized by covalently closed continuous loops.. Recently, circRNAs have recently shown large regulation on cardiovascular system, including atherosclerosis.. The present study aims to investigate the circRNA expression profile and identify their roles on vascular endothelial cells induced by oxLDL. Article Title: Comprehensive Analysis of circRNA Expression Profiles in Human Brown Adipose Tissue Article Snippet: The target microRNAs of hsa_circ_0006168 ( A ), hsa_circ_0026337 ( B ), hsa_circ_000750 ( C ), and Article Title: Circular RNA hsa_circ_0016788 regulates hepatocellular carcinoma tumorigenesis through miR-486/CDK4 pathway. Article Snippet: To investigate the potential regulatory mechanism of hsa_circ_0016788 on HCC, bioinformatics tools and Article Title: Circular RNA LPAR3 sponges microRNA‐198 to facilitate esophageal cancer migration, invasion, and metastasis Article Snippet: Using Article Title: Circular RNA Profiling Reveals Exosomal circ_0006156 as a Novel Biomarker in Papillary Thyroid Cancer Article Snippet: The Article Title: Exosome circ_0044516 promotes prostate cancer cell proliferation and metastasis as a potential biomarker. Article Snippet: Department of Urology, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China Minimally Invasive Technique and Product Translational Center, Guangzhou Medical University, Guangzhou, China Department of Urology, The First Affiliated Hospital of Sun Yat‐Sen University, Guangzhou, China Department of Biomedical Engineering, Jinan University, Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Guangzhou, China |