hoechst 33258  (Millipore)


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    Structured Review

    Millipore hoechst 33258
    Treatment with cisPt induced caspase activation, and DNA fragmentation demonstrated by TUNEL assay . TUNEL‐positive cells (b, green fluorescence) were counterstained with Evan's blue (red fluorescence); in (a) DNA was counterstained with Hoechst 33258.
    Hoechst 33258, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 824 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33258/product/Millipore
    Average 99 stars, based on 824 article reviews
    Price from $9.99 to $1999.99
    hoechst 33258 - by Bioz Stars, 2020-05
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    Images

    1) Product Images from "Cell proliferation, apoptosis and mitochondrial damage in rat B50 neuronal cells after cisplatin treatment"

    Article Title: Cell proliferation, apoptosis and mitochondrial damage in rat B50 neuronal cells after cisplatin treatment

    Journal: Cell Proliferation

    doi: 10.1111/j.1365-2184.2008.00530.x

    Treatment with cisPt induced caspase activation, and DNA fragmentation demonstrated by TUNEL assay . TUNEL‐positive cells (b, green fluorescence) were counterstained with Evan's blue (red fluorescence); in (a) DNA was counterstained with Hoechst 33258.
    Figure Legend Snippet: Treatment with cisPt induced caspase activation, and DNA fragmentation demonstrated by TUNEL assay . TUNEL‐positive cells (b, green fluorescence) were counterstained with Evan's blue (red fluorescence); in (a) DNA was counterstained with Hoechst 33258.

    Techniques Used: Activation Assay, TUNEL Assay, Fluorescence

    Confocal microscopy.  Immunocytochemical detection of Bax (green fluorescence) in control cells (a) and after cisPt treatment (b). Cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence) and DNA was counterstained with Hoechst 33258. Double immunoreaction for mitochondria (green fluorescence, c) and Bax (red fluorescence, d) in cisPt‐treated B50 cells, DNA counterstained with Hoechst 33258. The three different sections (e′, e″, e′″) represent the fluorescence distribution determined for sections of the cell, as indicated in the red/green fusion image (e). After treatment, Bax translocates from its predominantly cytoplasmic location to mitochondria and induces activation of apoptosis.
    Figure Legend Snippet: Confocal microscopy. Immunocytochemical detection of Bax (green fluorescence) in control cells (a) and after cisPt treatment (b). Cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence) and DNA was counterstained with Hoechst 33258. Double immunoreaction for mitochondria (green fluorescence, c) and Bax (red fluorescence, d) in cisPt‐treated B50 cells, DNA counterstained with Hoechst 33258. The three different sections (e′, e″, e′″) represent the fluorescence distribution determined for sections of the cell, as indicated in the red/green fusion image (e). After treatment, Bax translocates from its predominantly cytoplasmic location to mitochondria and induces activation of apoptosis.

    Techniques Used: Confocal Microscopy, Fluorescence, Activation Assay

    Confocal microscopy of dual immunolabelling of mitochondria (green fluorescence) and actinic cytoskeleton (red fluorescence) in B50 cells.  Compared to untreated controls (a), in cisPt‐treated cells (b), mitochondria clustered around the nucleus and formed dense masses in the cytoplasm. Control cells (a) revealed a filamentous actin skeleton; in (b) cisPt induced disruption of filamentous actin structures and assembly of depolymerized actin in peripheral regions of cytoplasm close to the cell membrane. DNA was counterstained with Hoechst 33258. Confocal microscopy: immunocytochemical detection of Bcl‐2 (green fluorescence) in control cells (c) and after cisPt treatment (d). After treatment with cisPt, Bcl‐2 protein levels increased significantly in the nucleus. The cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence).
    Figure Legend Snippet: Confocal microscopy of dual immunolabelling of mitochondria (green fluorescence) and actinic cytoskeleton (red fluorescence) in B50 cells. Compared to untreated controls (a), in cisPt‐treated cells (b), mitochondria clustered around the nucleus and formed dense masses in the cytoplasm. Control cells (a) revealed a filamentous actin skeleton; in (b) cisPt induced disruption of filamentous actin structures and assembly of depolymerized actin in peripheral regions of cytoplasm close to the cell membrane. DNA was counterstained with Hoechst 33258. Confocal microscopy: immunocytochemical detection of Bcl‐2 (green fluorescence) in control cells (c) and after cisPt treatment (d). After treatment with cisPt, Bcl‐2 protein levels increased significantly in the nucleus. The cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence).

    Techniques Used: Confocal Microscopy, Fluorescence

    2) Product Images from "Cell proliferation, apoptosis and mitochondrial damage in rat B50 neuronal cells after cisplatin treatment"

    Article Title: Cell proliferation, apoptosis and mitochondrial damage in rat B50 neuronal cells after cisplatin treatment

    Journal: Cell Proliferation

    doi: 10.1111/j.1365-2184.2008.00530.x

    Treatment with cisPt induced caspase activation, and DNA fragmentation demonstrated by TUNEL assay . TUNEL‐positive cells (b, green fluorescence) were counterstained with Evan's blue (red fluorescence); in (a) DNA was counterstained with Hoechst 33258.
    Figure Legend Snippet: Treatment with cisPt induced caspase activation, and DNA fragmentation demonstrated by TUNEL assay . TUNEL‐positive cells (b, green fluorescence) were counterstained with Evan's blue (red fluorescence); in (a) DNA was counterstained with Hoechst 33258.

    Techniques Used: Activation Assay, TUNEL Assay, Fluorescence

    Confocal microscopy.  Immunocytochemical detection of Bax (green fluorescence) in control cells (a) and after cisPt treatment (b). Cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence) and DNA was counterstained with Hoechst 33258. Double immunoreaction for mitochondria (green fluorescence, c) and Bax (red fluorescence, d) in cisPt‐treated B50 cells, DNA counterstained with Hoechst 33258. The three different sections (e′, e″, e′″) represent the fluorescence distribution determined for sections of the cell, as indicated in the red/green fusion image (e). After treatment, Bax translocates from its predominantly cytoplasmic location to mitochondria and induces activation of apoptosis.
    Figure Legend Snippet: Confocal microscopy. Immunocytochemical detection of Bax (green fluorescence) in control cells (a) and after cisPt treatment (b). Cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence) and DNA was counterstained with Hoechst 33258. Double immunoreaction for mitochondria (green fluorescence, c) and Bax (red fluorescence, d) in cisPt‐treated B50 cells, DNA counterstained with Hoechst 33258. The three different sections (e′, e″, e′″) represent the fluorescence distribution determined for sections of the cell, as indicated in the red/green fusion image (e). After treatment, Bax translocates from its predominantly cytoplasmic location to mitochondria and induces activation of apoptosis.

    Techniques Used: Confocal Microscopy, Fluorescence, Activation Assay

    Confocal microscopy of dual immunolabelling of mitochondria (green fluorescence) and actinic cytoskeleton (red fluorescence) in B50 cells.  Compared to untreated controls (a), in cisPt‐treated cells (b), mitochondria clustered around the nucleus and formed dense masses in the cytoplasm. Control cells (a) revealed a filamentous actin skeleton; in (b) cisPt induced disruption of filamentous actin structures and assembly of depolymerized actin in peripheral regions of cytoplasm close to the cell membrane. DNA was counterstained with Hoechst 33258. Confocal microscopy: immunocytochemical detection of Bcl‐2 (green fluorescence) in control cells (c) and after cisPt treatment (d). After treatment with cisPt, Bcl‐2 protein levels increased significantly in the nucleus. The cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence).
    Figure Legend Snippet: Confocal microscopy of dual immunolabelling of mitochondria (green fluorescence) and actinic cytoskeleton (red fluorescence) in B50 cells. Compared to untreated controls (a), in cisPt‐treated cells (b), mitochondria clustered around the nucleus and formed dense masses in the cytoplasm. Control cells (a) revealed a filamentous actin skeleton; in (b) cisPt induced disruption of filamentous actin structures and assembly of depolymerized actin in peripheral regions of cytoplasm close to the cell membrane. DNA was counterstained with Hoechst 33258. Confocal microscopy: immunocytochemical detection of Bcl‐2 (green fluorescence) in control cells (c) and after cisPt treatment (d). After treatment with cisPt, Bcl‐2 protein levels increased significantly in the nucleus. The cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence).

    Techniques Used: Confocal Microscopy, Fluorescence

    3) Product Images from "Neuroprotective Effects of Daphnetin against NMDA Receptor-Mediated Excitotoxicity"

    Article Title: Neuroprotective Effects of Daphnetin against NMDA Receptor-Mediated Excitotoxicity

    Journal: Molecules

    doi: 10.3390/molecules190914542

    Hoechst 33258 and PI double staining in cultured cortical neurons. ( A ) The representative fluorescence images obtained after Hoechst 33258, PI, and double staining in cortical neurons. Scale bar: 20 μm. ( B ) The percentage of apoptotic neurons in total neurons for control, NMDA, NMDA + Dap (10 μM), and Dap (10 μM) treated groups. The apoptotic numbers were counted from three independent experiments. * p
    Figure Legend Snippet: Hoechst 33258 and PI double staining in cultured cortical neurons. ( A ) The representative fluorescence images obtained after Hoechst 33258, PI, and double staining in cortical neurons. Scale bar: 20 μm. ( B ) The percentage of apoptotic neurons in total neurons for control, NMDA, NMDA + Dap (10 μM), and Dap (10 μM) treated groups. The apoptotic numbers were counted from three independent experiments. * p

    Techniques Used: Double Staining, Cell Culture, Fluorescence

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    Article Snippet: The pellet was resuspended in a 1:1 mixture of buffer A and His-Select wash buffer (10 mM imidazole, 0.3 M NaCl, 50 mM sodium phosphate, pH 8.0), and the suspension was applied in 200-mg batches to a 6.4-ml His-Select cartridge (Sigma-Aldrich).

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    Millipore hoechst 33258 dye
    ALS-FTD-CSF induces intracellular mislocalization and aggregation of endogenous TDP-43 in U251 cells (A) Subcellular redistribution of TDP-43 in U251 cells following incubation of CSF. Immunofluorescent staining for endogenous TDP-43 labeled with rabbit anti-TDP-43 antibody (red), and the F-actin cytoskeleton labeled with phalloidin-Alexa Fluor 488 (green) were examined by fluorescent microscopy. The nucleus was stained with <t>Hoechst</t> 33258 (blue). Both mislocalization of TDP-43 (arrow) and TDP-43 aggregates (arrowhead) were assembled in ALS-FTD-CSF-cultured U251 cells, but the changes were not exhibited in ALS-CSF-cultured cells. Representative images of a field (n=8) are shown for one of three independent experiments from each culture. (B) High magnification microphotographs of TDP-43 mislocalization in cells following incubation of ALS-FTD-CSF. The cytoplasmic TDP-43 inclusions formed in cells and TDP-43 was diffusely distributed from the nucleus to the cytoplasm in the ALS-FTD-CSF-cultured cells. An arrowhead indicates the TDP-43 aggregates and an arrow indicates the TDP-43 distributes in the whole cells. (C) The ratio of the intensity for the fluorescence of TDP-43 located in nucleus (black) to TDP-43 in cytoplasm (red) in the CSF-cultured cells. Each bar represents values averaged from 200 cells, using student's t test with Bonferroni correction. (D) The percentage of cells containing TDP-43 aggregates following cultured with CSF. Values shown are the mean ± SD from three experiments. Level of statistical significance: ** p
    Hoechst 33258 Dye, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hoechst 33258 dye/product/Millipore
    Average 99 stars, based on 117 article reviews
    Price from $9.99 to $1999.99
    hoechst 33258 dye - by Bioz Stars, 2020-05
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    99
    Millipore bisbenzimide
    Invasive and angiogenic properties of malignant mouse PDVA keratinocytes in vivo Cells were transplanted for three weeks into ( A , E ) wild-type, ( B , F ) MMP-2−/−, ( C , G ) MMP-9−/−, or ( D , H ) MMP-2−/−; MMP-9−/− mice. ( A – D ) Malignant cells were stained with antikeratin Ab (green) and vessels with anti-type-IV collagen (red). Collagen type-IV collagen labeling was codistributed with immunostaining of endothelial cells recognized by anti-mouse platelet endothelial cell adhesion molecule (data not shown). ( E – H ) In situ zymography-detecting gelatinolytic activity (green), cells were counterstained with <t>bisbenzimide</t> (blue). C: carcinoma cells; G: collagen gel: H: host tissue. Original magnification: ( A – D ) 200×, ( E – H ) 100×.
    Bisbenzimide, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1094 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bisbenzimide/product/Millipore
    Average 99 stars, based on 1094 article reviews
    Price from $9.99 to $1999.99
    bisbenzimide - by Bioz Stars, 2020-05
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    Image Search Results


    ALS-FTD-CSF induces intracellular mislocalization and aggregation of endogenous TDP-43 in U251 cells (A) Subcellular redistribution of TDP-43 in U251 cells following incubation of CSF. Immunofluorescent staining for endogenous TDP-43 labeled with rabbit anti-TDP-43 antibody (red), and the F-actin cytoskeleton labeled with phalloidin-Alexa Fluor 488 (green) were examined by fluorescent microscopy. The nucleus was stained with Hoechst 33258 (blue). Both mislocalization of TDP-43 (arrow) and TDP-43 aggregates (arrowhead) were assembled in ALS-FTD-CSF-cultured U251 cells, but the changes were not exhibited in ALS-CSF-cultured cells. Representative images of a field (n=8) are shown for one of three independent experiments from each culture. (B) High magnification microphotographs of TDP-43 mislocalization in cells following incubation of ALS-FTD-CSF. The cytoplasmic TDP-43 inclusions formed in cells and TDP-43 was diffusely distributed from the nucleus to the cytoplasm in the ALS-FTD-CSF-cultured cells. An arrowhead indicates the TDP-43 aggregates and an arrow indicates the TDP-43 distributes in the whole cells. (C) The ratio of the intensity for the fluorescence of TDP-43 located in nucleus (black) to TDP-43 in cytoplasm (red) in the CSF-cultured cells. Each bar represents values averaged from 200 cells, using student's t test with Bonferroni correction. (D) The percentage of cells containing TDP-43 aggregates following cultured with CSF. Values shown are the mean ± SD from three experiments. Level of statistical significance: ** p

    Journal: Oncotarget

    Article Title: Exposure to ALS-FTD-CSF generates TDP-43 aggregates in glioblastoma cells through exosomes and TNTs-like structure

    doi:

    Figure Lengend Snippet: ALS-FTD-CSF induces intracellular mislocalization and aggregation of endogenous TDP-43 in U251 cells (A) Subcellular redistribution of TDP-43 in U251 cells following incubation of CSF. Immunofluorescent staining for endogenous TDP-43 labeled with rabbit anti-TDP-43 antibody (red), and the F-actin cytoskeleton labeled with phalloidin-Alexa Fluor 488 (green) were examined by fluorescent microscopy. The nucleus was stained with Hoechst 33258 (blue). Both mislocalization of TDP-43 (arrow) and TDP-43 aggregates (arrowhead) were assembled in ALS-FTD-CSF-cultured U251 cells, but the changes were not exhibited in ALS-CSF-cultured cells. Representative images of a field (n=8) are shown for one of three independent experiments from each culture. (B) High magnification microphotographs of TDP-43 mislocalization in cells following incubation of ALS-FTD-CSF. The cytoplasmic TDP-43 inclusions formed in cells and TDP-43 was diffusely distributed from the nucleus to the cytoplasm in the ALS-FTD-CSF-cultured cells. An arrowhead indicates the TDP-43 aggregates and an arrow indicates the TDP-43 distributes in the whole cells. (C) The ratio of the intensity for the fluorescence of TDP-43 located in nucleus (black) to TDP-43 in cytoplasm (red) in the CSF-cultured cells. Each bar represents values averaged from 200 cells, using student's t test with Bonferroni correction. (D) The percentage of cells containing TDP-43 aggregates following cultured with CSF. Values shown are the mean ± SD from three experiments. Level of statistical significance: ** p

    Article Snippet: Nuclei were stained with Hoechst 33258 dye (Calbiochem, San Diego, CA) at the concentration of 1 μg/ml for 5 min. To stain for TDP-43, the fixed cells were incubated with a rabbit polyclonal antibody (1:100; ProteinTech Group, Inc, Chicago, IL) in the blocking buffer at 4°C overnight.

    Techniques: Incubation, Staining, Labeling, Microscopy, Cell Culture, Fluorescence

    Increased expression and mislocalization of α-syn in U251 following exposure to MSA-CSF A. Subcellular redistribution of α-syn in U251 cells following incubation of CSF. Immunofluorescent staining for endogenous α-syn labeled with anti-α-syn antibody (red), and the F-actin cytoskeleton labeled with phalloidin-Alexa Fluor 488 (green) were examined by fluorescent microscopy. The nucleus was stained with Hoechst 33258 (blue). B. High magnification microphotographs of α-syn distribution in U251 cells following incubation of CSF. U251 cells were deformed and endogenous α-syn tends to distribute to peri-nuclear in the MSA-CSF-cultured U251 cells.

    Journal: Oncotarget

    Article Title: Chronic exposure to cerebrospinal fluid of multiple system atrophy in neuroblastoma and glioblastoma cells induces cytotoxicity via ER stress and autophagy activation

    doi:

    Figure Lengend Snippet: Increased expression and mislocalization of α-syn in U251 following exposure to MSA-CSF A. Subcellular redistribution of α-syn in U251 cells following incubation of CSF. Immunofluorescent staining for endogenous α-syn labeled with anti-α-syn antibody (red), and the F-actin cytoskeleton labeled with phalloidin-Alexa Fluor 488 (green) were examined by fluorescent microscopy. The nucleus was stained with Hoechst 33258 (blue). B. High magnification microphotographs of α-syn distribution in U251 cells following incubation of CSF. U251 cells were deformed and endogenous α-syn tends to distribute to peri-nuclear in the MSA-CSF-cultured U251 cells.

    Article Snippet: Nuclei were stained with Hoechst 33258 dye (Calbiochem, San Diego, CA; 0.5 μg/ml) for 5 min. To stain α-syn, the fixed cells were incubated with a goat polyclonal antibody (R & D Systems, Inc. Minneapolis, MN; 1:50) in the blocking buffer at 4°C overnight.

    Techniques: Expressing, Incubation, Staining, Labeling, Microscopy, Cell Culture

    Increased expression and mislocalization of α-syn in SH-SY5Y following exposure to MSA-CSF A. Subcellular redistribution of α-syn in SH-SY5Y cells following incubation of CSF. Immunofluorescent staining for endogenous α-syn labeled with goat anti-α-syn antibody (red), and the F-actin cytoskeleton labeled with phalloidin-Alexa Fluor 488 (green) were examined by fluorescent microscopy. The nucleus was stained with Hoechst 33258 (blue). B. High magnification microphotographs of α-syn distribution in SH-SY5Y cells following incubation of CSF.

    Journal: Oncotarget

    Article Title: Chronic exposure to cerebrospinal fluid of multiple system atrophy in neuroblastoma and glioblastoma cells induces cytotoxicity via ER stress and autophagy activation

    doi:

    Figure Lengend Snippet: Increased expression and mislocalization of α-syn in SH-SY5Y following exposure to MSA-CSF A. Subcellular redistribution of α-syn in SH-SY5Y cells following incubation of CSF. Immunofluorescent staining for endogenous α-syn labeled with goat anti-α-syn antibody (red), and the F-actin cytoskeleton labeled with phalloidin-Alexa Fluor 488 (green) were examined by fluorescent microscopy. The nucleus was stained with Hoechst 33258 (blue). B. High magnification microphotographs of α-syn distribution in SH-SY5Y cells following incubation of CSF.

    Article Snippet: Nuclei were stained with Hoechst 33258 dye (Calbiochem, San Diego, CA; 0.5 μg/ml) for 5 min. To stain α-syn, the fixed cells were incubated with a goat polyclonal antibody (R & D Systems, Inc. Minneapolis, MN; 1:50) in the blocking buffer at 4°C overnight.

    Techniques: Expressing, Incubation, Staining, Labeling, Microscopy

    Invasive and angiogenic properties of malignant mouse PDVA keratinocytes in vivo Cells were transplanted for three weeks into ( A , E ) wild-type, ( B , F ) MMP-2−/−, ( C , G ) MMP-9−/−, or ( D , H ) MMP-2−/−; MMP-9−/− mice. ( A – D ) Malignant cells were stained with antikeratin Ab (green) and vessels with anti-type-IV collagen (red). Collagen type-IV collagen labeling was codistributed with immunostaining of endothelial cells recognized by anti-mouse platelet endothelial cell adhesion molecule (data not shown). ( E – H ) In situ zymography-detecting gelatinolytic activity (green), cells were counterstained with bisbenzimide (blue). C: carcinoma cells; G: collagen gel: H: host tissue. Original magnification: ( A – D ) 200×, ( E – H ) 100×.

    Journal: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Contribution of host MMP-2 and MMP-9 to promote tumor vascularization and invasion of malignant keratinocytes

    doi: 10.1096/fj.04-2140fje

    Figure Lengend Snippet: Invasive and angiogenic properties of malignant mouse PDVA keratinocytes in vivo Cells were transplanted for three weeks into ( A , E ) wild-type, ( B , F ) MMP-2−/−, ( C , G ) MMP-9−/−, or ( D , H ) MMP-2−/−; MMP-9−/− mice. ( A – D ) Malignant cells were stained with antikeratin Ab (green) and vessels with anti-type-IV collagen (red). Collagen type-IV collagen labeling was codistributed with immunostaining of endothelial cells recognized by anti-mouse platelet endothelial cell adhesion molecule (data not shown). ( E – H ) In situ zymography-detecting gelatinolytic activity (green), cells were counterstained with bisbenzimide (blue). C: carcinoma cells; G: collagen gel: H: host tissue. Original magnification: ( A – D ) 200×, ( E – H ) 100×.

    Article Snippet: Cells were counterstained using bisbenzimide (Calbiochem; fluorochromo-3HCl, 5 H2 O; dilution 1/100).

    Techniques: In Vivo, Mouse Assay, Staining, Labeling, Immunostaining, In Situ, Zymography, Activity Assay

    Treatment with cisPt induced caspase activation, and DNA fragmentation demonstrated by TUNEL assay . TUNEL‐positive cells (b, green fluorescence) were counterstained with Evan's blue (red fluorescence); in (a) DNA was counterstained with Hoechst 33258.

    Journal: Cell Proliferation

    Article Title: Cell proliferation, apoptosis and mitochondrial damage in rat B50 neuronal cells after cisplatin treatment

    doi: 10.1111/j.1365-2184.2008.00530.x

    Figure Lengend Snippet: Treatment with cisPt induced caspase activation, and DNA fragmentation demonstrated by TUNEL assay . TUNEL‐positive cells (b, green fluorescence) were counterstained with Evan's blue (red fluorescence); in (a) DNA was counterstained with Hoechst 33258.

    Article Snippet: Sections were counterstained for DNA with 0.1 µg/mL Hoechst 33258, washed with PBS and finally mounted in Mowiol (Calbiochem), for confocal microscopy analysis.

    Techniques: Activation Assay, TUNEL Assay, Fluorescence

    Confocal microscopy.  Immunocytochemical detection of Bax (green fluorescence) in control cells (a) and after cisPt treatment (b). Cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence) and DNA was counterstained with Hoechst 33258. Double immunoreaction for mitochondria (green fluorescence, c) and Bax (red fluorescence, d) in cisPt‐treated B50 cells, DNA counterstained with Hoechst 33258. The three different sections (e′, e″, e′″) represent the fluorescence distribution determined for sections of the cell, as indicated in the red/green fusion image (e). After treatment, Bax translocates from its predominantly cytoplasmic location to mitochondria and induces activation of apoptosis.

    Journal: Cell Proliferation

    Article Title: Cell proliferation, apoptosis and mitochondrial damage in rat B50 neuronal cells after cisplatin treatment

    doi: 10.1111/j.1365-2184.2008.00530.x

    Figure Lengend Snippet: Confocal microscopy. Immunocytochemical detection of Bax (green fluorescence) in control cells (a) and after cisPt treatment (b). Cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence) and DNA was counterstained with Hoechst 33258. Double immunoreaction for mitochondria (green fluorescence, c) and Bax (red fluorescence, d) in cisPt‐treated B50 cells, DNA counterstained with Hoechst 33258. The three different sections (e′, e″, e′″) represent the fluorescence distribution determined for sections of the cell, as indicated in the red/green fusion image (e). After treatment, Bax translocates from its predominantly cytoplasmic location to mitochondria and induces activation of apoptosis.

    Article Snippet: Sections were counterstained for DNA with 0.1 µg/mL Hoechst 33258, washed with PBS and finally mounted in Mowiol (Calbiochem), for confocal microscopy analysis.

    Techniques: Confocal Microscopy, Fluorescence, Activation Assay

    Confocal microscopy of dual immunolabelling of mitochondria (green fluorescence) and actinic cytoskeleton (red fluorescence) in B50 cells.  Compared to untreated controls (a), in cisPt‐treated cells (b), mitochondria clustered around the nucleus and formed dense masses in the cytoplasm. Control cells (a) revealed a filamentous actin skeleton; in (b) cisPt induced disruption of filamentous actin structures and assembly of depolymerized actin in peripheral regions of cytoplasm close to the cell membrane. DNA was counterstained with Hoechst 33258. Confocal microscopy: immunocytochemical detection of Bcl‐2 (green fluorescence) in control cells (c) and after cisPt treatment (d). After treatment with cisPt, Bcl‐2 protein levels increased significantly in the nucleus. The cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence).

    Journal: Cell Proliferation

    Article Title: Cell proliferation, apoptosis and mitochondrial damage in rat B50 neuronal cells after cisplatin treatment

    doi: 10.1111/j.1365-2184.2008.00530.x

    Figure Lengend Snippet: Confocal microscopy of dual immunolabelling of mitochondria (green fluorescence) and actinic cytoskeleton (red fluorescence) in B50 cells. Compared to untreated controls (a), in cisPt‐treated cells (b), mitochondria clustered around the nucleus and formed dense masses in the cytoplasm. Control cells (a) revealed a filamentous actin skeleton; in (b) cisPt induced disruption of filamentous actin structures and assembly of depolymerized actin in peripheral regions of cytoplasm close to the cell membrane. DNA was counterstained with Hoechst 33258. Confocal microscopy: immunocytochemical detection of Bcl‐2 (green fluorescence) in control cells (c) and after cisPt treatment (d). After treatment with cisPt, Bcl‐2 protein levels increased significantly in the nucleus. The cytoskeleton was labelled with Alexa 594‐conjugated phalloidin (red fluorescence).

    Article Snippet: Sections were counterstained for DNA with 0.1 µg/mL Hoechst 33258, washed with PBS and finally mounted in Mowiol (Calbiochem), for confocal microscopy analysis.

    Techniques: Confocal Microscopy, Fluorescence