hmgb1 detection kit  (Chondrex Inc)


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    Chondrex Inc hmgb1 detection kit
    In vitro immune cell death (ICD) induced by ROS generated from the Fenton-like reaction under the NIR II irradiation. ( a ) Schematic illustration of the DAMPs (CRT, ATP and <t>HMGB1)</t> released from the dying tumor cells to promote the DC mature, triggered by large amount of ROS generated from the degradation of H 2 O 2 by CS-I/J@CM NPs under irradiation. ( b ) CLSM of CRT (red fluorescence) exposed on the cytomembrane (green fluorescence) of GL261 cells, after different treatment with or without CS-I/J@CM NPs (12.5 μg/mL) (scale bar: 20 μm). ( c ) Colocalization of CRT and cytomembrane of GL261 cells. ( d ) Flow cytometry analysis of CRT expose on GL261 cells after co-cultured with or without CS-I/J@CM NPs (12.5 μg/mL). ( e ) Detection of extracellular ATP secreted. ( f ) Detection of extracellular HMGB1 released. ( g ) Flow cytometry analysis of the maturation of DC (CD11c + CD80 + CD86 + ). ( h ) Normalization of the maturation of DC in Fig. 3 g. (*p
    Hmgb1 Detection Kit, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmgb1 detection kit/product/Chondrex Inc
    Average 94 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    hmgb1 detection kit - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "Biomimetic nanoparticles directly remodel immunosuppressive microenvironment for boosting glioblastoma immunotherapy"

    Article Title: Biomimetic nanoparticles directly remodel immunosuppressive microenvironment for boosting glioblastoma immunotherapy

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2021.12.029

    In vitro immune cell death (ICD) induced by ROS generated from the Fenton-like reaction under the NIR II irradiation. ( a ) Schematic illustration of the DAMPs (CRT, ATP and HMGB1) released from the dying tumor cells to promote the DC mature, triggered by large amount of ROS generated from the degradation of H 2 O 2 by CS-I/J@CM NPs under irradiation. ( b ) CLSM of CRT (red fluorescence) exposed on the cytomembrane (green fluorescence) of GL261 cells, after different treatment with or without CS-I/J@CM NPs (12.5 μg/mL) (scale bar: 20 μm). ( c ) Colocalization of CRT and cytomembrane of GL261 cells. ( d ) Flow cytometry analysis of CRT expose on GL261 cells after co-cultured with or without CS-I/J@CM NPs (12.5 μg/mL). ( e ) Detection of extracellular ATP secreted. ( f ) Detection of extracellular HMGB1 released. ( g ) Flow cytometry analysis of the maturation of DC (CD11c + CD80 + CD86 + ). ( h ) Normalization of the maturation of DC in Fig. 3 g. (*p
    Figure Legend Snippet: In vitro immune cell death (ICD) induced by ROS generated from the Fenton-like reaction under the NIR II irradiation. ( a ) Schematic illustration of the DAMPs (CRT, ATP and HMGB1) released from the dying tumor cells to promote the DC mature, triggered by large amount of ROS generated from the degradation of H 2 O 2 by CS-I/J@CM NPs under irradiation. ( b ) CLSM of CRT (red fluorescence) exposed on the cytomembrane (green fluorescence) of GL261 cells, after different treatment with or without CS-I/J@CM NPs (12.5 μg/mL) (scale bar: 20 μm). ( c ) Colocalization of CRT and cytomembrane of GL261 cells. ( d ) Flow cytometry analysis of CRT expose on GL261 cells after co-cultured with or without CS-I/J@CM NPs (12.5 μg/mL). ( e ) Detection of extracellular ATP secreted. ( f ) Detection of extracellular HMGB1 released. ( g ) Flow cytometry analysis of the maturation of DC (CD11c + CD80 + CD86 + ). ( h ) Normalization of the maturation of DC in Fig. 3 g. (*p

    Techniques Used: In Vitro, Generated, Irradiation, Confocal Laser Scanning Microscopy, Fluorescence, Flow Cytometry, Cell Culture

    2) Product Images from "A microengineered model of RBC transfusion-induced pulmonary vascular injury"

    Article Title: A microengineered model of RBC transfusion-induced pulmonary vascular injury

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-03597-w

    Effect of hemodynamic shear stress on transfusion-induced endothelial injury. ( A , B ) Endothelial perfusion with RBCs at a sub-physiological level of shear stress (0.14 dyn/cm 2 ) gives rise to marked downregulation of nuclear HMGB1 expression (“low shear transfusion”), indicating severe necroptosis in the microvascular endothelium (*** p
    Figure Legend Snippet: Effect of hemodynamic shear stress on transfusion-induced endothelial injury. ( A , B ) Endothelial perfusion with RBCs at a sub-physiological level of shear stress (0.14 dyn/cm 2 ) gives rise to marked downregulation of nuclear HMGB1 expression (“low shear transfusion”), indicating severe necroptosis in the microvascular endothelium (*** p

    Techniques Used: Expressing

    RBC transfusion-induced acute vascular injury. ( A ) Continuous RBC transfusion for 4 hours results in a loss of nuclear HMGB1 (red). Control data show HMGB1 expression in endothelial cells perfused with culture medium alone for the same period. Nuclei are stained with DAPI (blue). ( B ) Quantification of nuclear HMGB1 staining under control (without RBCs) and transfusion conditions (*** p
    Figure Legend Snippet: RBC transfusion-induced acute vascular injury. ( A ) Continuous RBC transfusion for 4 hours results in a loss of nuclear HMGB1 (red). Control data show HMGB1 expression in endothelial cells perfused with culture medium alone for the same period. Nuclei are stained with DAPI (blue). ( B ) Quantification of nuclear HMGB1 staining under control (without RBCs) and transfusion conditions (*** p

    Techniques Used: Expressing, Staining

    Effect of mechanical stretch on transfusion-induced vascular injury. ( A ) The cell stretching system consists of grippers and motorized linear actuators that hold and stretch our cell culture microfluidic device in the lateral direction during perfusion experiments. ( B ) The entire platform can be mounted on a microscope stage for real-time monitoring and imaging of our model. ( C ) Uni-axial stretching of the microchannel by computer-controlled stretching system (Scale bar: 50 μm). The same microchannel was pseudo-colored at different levels of strain to show stretch-induced widening of the channel. ( D ) Quantification of extracellular HMGB1 measured in the fresh RBC units and perfusate from the control and transfusion groups in the presence and absence of cyclic stretch. Increases in the level of extracellular HMGB1 due to RBC transfusion are substantially higher when the endothelium is mechanically stretched at physiological strain (10%) and frequency (0.2 Hz) (*** p
    Figure Legend Snippet: Effect of mechanical stretch on transfusion-induced vascular injury. ( A ) The cell stretching system consists of grippers and motorized linear actuators that hold and stretch our cell culture microfluidic device in the lateral direction during perfusion experiments. ( B ) The entire platform can be mounted on a microscope stage for real-time monitoring and imaging of our model. ( C ) Uni-axial stretching of the microchannel by computer-controlled stretching system (Scale bar: 50 μm). The same microchannel was pseudo-colored at different levels of strain to show stretch-induced widening of the channel. ( D ) Quantification of extracellular HMGB1 measured in the fresh RBC units and perfusate from the control and transfusion groups in the presence and absence of cyclic stretch. Increases in the level of extracellular HMGB1 due to RBC transfusion are substantially higher when the endothelium is mechanically stretched at physiological strain (10%) and frequency (0.2 Hz) (*** p

    Techniques Used: Cell Culture, Microscopy, Imaging

    3) Product Images from "Intra-Peritoneal Administration of Mitochondrial DNA Provokes Acute Lung Injury and Systemic Inflammation via Toll-Like Receptor 9"

    Article Title: Intra-Peritoneal Administration of Mitochondrial DNA Provokes Acute Lung Injury and Systemic Inflammation via Toll-Like Receptor 9

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms17091425

    Intra-peritoneal administration of mtDNA induces acute lung injury and systemic inflammation in a TLR9-dependent manner. WT mice were randomly assigned to a group pretreated with the TLR9 specific inhibitor ODN2088 or a control group. Mice in the ODN2088 were pretreated 1 h before mtDNA administration ( n = 16/group, 8 for BALF only). Acute lung injury was measured by HE staining (scale bar is 100 μm) ( A ) for lung injury score ( B ); BALF total protein concentration ( C ); and lung W/D ratio ( D ) were also analyzed. Systemic inflammation and circulating levels of IL-1β ( E ); IL-6 ( F ); and HMGB1 ( G ) were measured by ELISA. * p
    Figure Legend Snippet: Intra-peritoneal administration of mtDNA induces acute lung injury and systemic inflammation in a TLR9-dependent manner. WT mice were randomly assigned to a group pretreated with the TLR9 specific inhibitor ODN2088 or a control group. Mice in the ODN2088 were pretreated 1 h before mtDNA administration ( n = 16/group, 8 for BALF only). Acute lung injury was measured by HE staining (scale bar is 100 μm) ( A ) for lung injury score ( B ); BALF total protein concentration ( C ); and lung W/D ratio ( D ) were also analyzed. Systemic inflammation and circulating levels of IL-1β ( E ); IL-6 ( F ); and HMGB1 ( G ) were measured by ELISA. * p

    Techniques Used: Mouse Assay, Staining, Protein Concentration, Enzyme-linked Immunosorbent Assay

    Intra-peritoneal administration of mtDNA induces acute lung injury and systemic inflammation in a TLR4-independent manner. WT and TLR4 KO mice were given NS, nDNA, and mtDNA administered intra-peritoneally ( n = 16/group, 8 for BALF only). Eight hours later, acute lung injury was measure by HE staining (scale bar is 100 μm) ( A ) for lung injury score ( B ); BALF total protein concentration ( C ); and lung W/D ratio ( D ) were also analyzed. Systemic inflammation and circulating levels of IL-1β ( E ); IL-6 ( F ); and HMGB1 ( G ) were measured by ELISA. + p > 0.05 versus WT/mtDNA group. Eight mice were used in each set and data are mean ± SEM of three separate experiments.
    Figure Legend Snippet: Intra-peritoneal administration of mtDNA induces acute lung injury and systemic inflammation in a TLR4-independent manner. WT and TLR4 KO mice were given NS, nDNA, and mtDNA administered intra-peritoneally ( n = 16/group, 8 for BALF only). Eight hours later, acute lung injury was measure by HE staining (scale bar is 100 μm) ( A ) for lung injury score ( B ); BALF total protein concentration ( C ); and lung W/D ratio ( D ) were also analyzed. Systemic inflammation and circulating levels of IL-1β ( E ); IL-6 ( F ); and HMGB1 ( G ) were measured by ELISA. + p > 0.05 versus WT/mtDNA group. Eight mice were used in each set and data are mean ± SEM of three separate experiments.

    Techniques Used: Mouse Assay, Staining, Protein Concentration, Enzyme-linked Immunosorbent Assay

    Intra-peritoneal administration of mtDNA leads to systemic inflammation. WT mice were treated with PBS, nuclear DNA (nDNA), or mtDNA via intra-peritoneal injection for 2, 8, and 24 h. Systemic inflammation and circulating levels of IL-1β ( A ); IL-6 ( B ); and HMGB1 ( C ) were measured by ELISA. * p
    Figure Legend Snippet: Intra-peritoneal administration of mtDNA leads to systemic inflammation. WT mice were treated with PBS, nuclear DNA (nDNA), or mtDNA via intra-peritoneal injection for 2, 8, and 24 h. Systemic inflammation and circulating levels of IL-1β ( A ); IL-6 ( B ); and HMGB1 ( C ) were measured by ELISA. * p

    Techniques Used: Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay

    4) Product Images from "Nano-Pulse Stimulation is a physical modality that can trigger immunogenic tumor cell death"

    Article Title: Nano-Pulse Stimulation is a physical modality that can trigger immunogenic tumor cell death

    Journal: Journal for Immunotherapy of Cancer

    doi: 10.1186/s40425-017-0234-5

    HMGB1 released by three cell lines 24 h after treatment with either NPS, or DOX or MTX. The number of pulses applied to achieve the indicated J/mL for all cell lines are indicated above the MCA205 plot. Significant difference from untreated controls by one-way ANOVA with between group analysis performed using the Dunnett’s test. * p
    Figure Legend Snippet: HMGB1 released by three cell lines 24 h after treatment with either NPS, or DOX or MTX. The number of pulses applied to achieve the indicated J/mL for all cell lines are indicated above the MCA205 plot. Significant difference from untreated controls by one-way ANOVA with between group analysis performed using the Dunnett’s test. * p

    Techniques Used:

    5) Product Images from "Inhibition of Alveolar Macrophage Pyroptosis Reduces Lipopolysaccharide-induced Acute Lung Injury in Mice"

    Article Title: Inhibition of Alveolar Macrophage Pyroptosis Reduces Lipopolysaccharide-induced Acute Lung Injury in Mice

    Journal: Chinese Medical Journal

    doi: 10.4103/0366-6999.166039

    The influences of YVAD and DEVD treatment of LPS-induced cytokine levels in plasma and bronchoalveolar lavage fluid. After YVAD and DEVD treatment and LPS injection at predetermined time points, serum and bronchoalveolar lavage fluid samples were prepared and tested for levels for IL-18, IL-1β, TNF-α and HMGB1 in serum (a–d) and bronchoalveolar lavage fluid (e–h). * P
    Figure Legend Snippet: The influences of YVAD and DEVD treatment of LPS-induced cytokine levels in plasma and bronchoalveolar lavage fluid. After YVAD and DEVD treatment and LPS injection at predetermined time points, serum and bronchoalveolar lavage fluid samples were prepared and tested for levels for IL-18, IL-1β, TNF-α and HMGB1 in serum (a–d) and bronchoalveolar lavage fluid (e–h). * P

    Techniques Used: Injection

    6) Product Images from "Beneficial effect of STAT3 decoy oligodeoxynucleotide transfection on organ injury and mortality in mice with cecal ligation and puncture-induced sepsis"

    Article Title: Beneficial effect of STAT3 decoy oligodeoxynucleotide transfection on organ injury and mortality in mice with cecal ligation and puncture-induced sepsis

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-72136-x

    Effect of STAT3 decoy ODN transfection on upregulated HMGB1 levels in mice following CLP-induced sepsis. ( A ) HMGB1 protein levels in lung and liver tissues. Typical Western blots are shown in the top images in each panel. HMGB1 protein levels were normalized to GAPDH ( n = 6). ( B ) HMGB1 mRNA in lung, liver, and kidney tissues. The mRNA levels of HMGB1 were quantified by real-time PCR and was normalized to GAPDH ( n = 6). Tissues were harvested 18 h after surgery. ( C ) Serum HMGB1 levels in 18-h CLP mice ( n = 6). * P
    Figure Legend Snippet: Effect of STAT3 decoy ODN transfection on upregulated HMGB1 levels in mice following CLP-induced sepsis. ( A ) HMGB1 protein levels in lung and liver tissues. Typical Western blots are shown in the top images in each panel. HMGB1 protein levels were normalized to GAPDH ( n = 6). ( B ) HMGB1 mRNA in lung, liver, and kidney tissues. The mRNA levels of HMGB1 were quantified by real-time PCR and was normalized to GAPDH ( n = 6). Tissues were harvested 18 h after surgery. ( C ) Serum HMGB1 levels in 18-h CLP mice ( n = 6). * P

    Techniques Used: Transfection, Mouse Assay, Western Blot, Real-time Polymerase Chain Reaction

    7) Product Images from "Bleomycin Exerts Ambivalent Antitumor Immune Effect by Triggering Both Immunogenic Cell Death and Proliferation of Regulatory T Cells"

    Article Title: Bleomycin Exerts Ambivalent Antitumor Immune Effect by Triggering Both Immunogenic Cell Death and Proliferation of Regulatory T Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0065181

    BLM treatment triggers characteristic events of immunogenic cell death. A: Western blotting analysis of phosphorylated, total eIF2α and β-actin in CT26 treated 24 h with the indicated drugs. B: indicated tumor cells were treated in vitro with different chemotherapeutic drugs for 24 h, then CRT (left panel) or ERp57 (right panel) exposure was detected by flow cytometry. C: tumor cells were treated with BLM, with or without N-acetylcystein (NAC) for 24 h, then CRT (left panel) or ERp57 (right panel) exposure was detected by flow cytometry. C: HMGB1 was titrated with ELISA method in supernatant of CT26 cells treated 24 h with indicated drugs. E: CT26 cells were cultured on glass slides and treated with indicated drugs for 24 h. The cells were then submitted to anti-LC3 (green) and DAPI (blue) labeling and observed under an epifluorescence microscope. F: CT26 cells were treated with indicated drugs for 24 h, then stained using Cyto-ID autophagy detection kit and analysed by flow cytometry. G: ATP was titrated in supernatant of CT26 treated with indicated drugs for 24 h. All data presented are representative of one out of three experiments. B-D, E and F show mean +/− SEM.
    Figure Legend Snippet: BLM treatment triggers characteristic events of immunogenic cell death. A: Western blotting analysis of phosphorylated, total eIF2α and β-actin in CT26 treated 24 h with the indicated drugs. B: indicated tumor cells were treated in vitro with different chemotherapeutic drugs for 24 h, then CRT (left panel) or ERp57 (right panel) exposure was detected by flow cytometry. C: tumor cells were treated with BLM, with or without N-acetylcystein (NAC) for 24 h, then CRT (left panel) or ERp57 (right panel) exposure was detected by flow cytometry. C: HMGB1 was titrated with ELISA method in supernatant of CT26 cells treated 24 h with indicated drugs. E: CT26 cells were cultured on glass slides and treated with indicated drugs for 24 h. The cells were then submitted to anti-LC3 (green) and DAPI (blue) labeling and observed under an epifluorescence microscope. F: CT26 cells were treated with indicated drugs for 24 h, then stained using Cyto-ID autophagy detection kit and analysed by flow cytometry. G: ATP was titrated in supernatant of CT26 treated with indicated drugs for 24 h. All data presented are representative of one out of three experiments. B-D, E and F show mean +/− SEM.

    Techniques Used: Western Blot, In Vitro, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay, Cell Culture, Labeling, Microscopy, Staining

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    Chondrex Inc hmgb1 detection kit
    In vitro immune cell death (ICD) induced by ROS generated from the Fenton-like reaction under the NIR II irradiation. ( a ) Schematic illustration of the DAMPs (CRT, ATP and <t>HMGB1)</t> released from the dying tumor cells to promote the DC mature, triggered by large amount of ROS generated from the degradation of H 2 O 2 by CS-I/J@CM NPs under irradiation. ( b ) CLSM of CRT (red fluorescence) exposed on the cytomembrane (green fluorescence) of GL261 cells, after different treatment with or without CS-I/J@CM NPs (12.5 μg/mL) (scale bar: 20 μm). ( c ) Colocalization of CRT and cytomembrane of GL261 cells. ( d ) Flow cytometry analysis of CRT expose on GL261 cells after co-cultured with or without CS-I/J@CM NPs (12.5 μg/mL). ( e ) Detection of extracellular ATP secreted. ( f ) Detection of extracellular HMGB1 released. ( g ) Flow cytometry analysis of the maturation of DC (CD11c + CD80 + CD86 + ). ( h ) Normalization of the maturation of DC in Fig. 3 g. (*p
    Hmgb1 Detection Kit, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmgb1 detection kit/product/Chondrex Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hmgb1 detection kit - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

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    In vitro immune cell death (ICD) induced by ROS generated from the Fenton-like reaction under the NIR II irradiation. ( a ) Schematic illustration of the DAMPs (CRT, ATP and HMGB1) released from the dying tumor cells to promote the DC mature, triggered by large amount of ROS generated from the degradation of H 2 O 2 by CS-I/J@CM NPs under irradiation. ( b ) CLSM of CRT (red fluorescence) exposed on the cytomembrane (green fluorescence) of GL261 cells, after different treatment with or without CS-I/J@CM NPs (12.5 μg/mL) (scale bar: 20 μm). ( c ) Colocalization of CRT and cytomembrane of GL261 cells. ( d ) Flow cytometry analysis of CRT expose on GL261 cells after co-cultured with or without CS-I/J@CM NPs (12.5 μg/mL). ( e ) Detection of extracellular ATP secreted. ( f ) Detection of extracellular HMGB1 released. ( g ) Flow cytometry analysis of the maturation of DC (CD11c + CD80 + CD86 + ). ( h ) Normalization of the maturation of DC in Fig. 3 g. (*p

    Journal: Bioactive Materials

    Article Title: Biomimetic nanoparticles directly remodel immunosuppressive microenvironment for boosting glioblastoma immunotherapy

    doi: 10.1016/j.bioactmat.2021.12.029

    Figure Lengend Snippet: In vitro immune cell death (ICD) induced by ROS generated from the Fenton-like reaction under the NIR II irradiation. ( a ) Schematic illustration of the DAMPs (CRT, ATP and HMGB1) released from the dying tumor cells to promote the DC mature, triggered by large amount of ROS generated from the degradation of H 2 O 2 by CS-I/J@CM NPs under irradiation. ( b ) CLSM of CRT (red fluorescence) exposed on the cytomembrane (green fluorescence) of GL261 cells, after different treatment with or without CS-I/J@CM NPs (12.5 μg/mL) (scale bar: 20 μm). ( c ) Colocalization of CRT and cytomembrane of GL261 cells. ( d ) Flow cytometry analysis of CRT expose on GL261 cells after co-cultured with or without CS-I/J@CM NPs (12.5 μg/mL). ( e ) Detection of extracellular ATP secreted. ( f ) Detection of extracellular HMGB1 released. ( g ) Flow cytometry analysis of the maturation of DC (CD11c + CD80 + CD86 + ). ( h ) Normalization of the maturation of DC in Fig. 3 g. (*p

    Article Snippet: The extracellularly released adenine nucleoside triphosphate (ATP) and high-mobility group box 1 (HMGB1) were detected by ATP detection Kit (A22066) (Molecular Probes) and HMGB1 detection Kit (Chondrex), respectively.

    Techniques: In Vitro, Generated, Irradiation, Confocal Laser Scanning Microscopy, Fluorescence, Flow Cytometry, Cell Culture

    Effect of hemodynamic shear stress on transfusion-induced endothelial injury. ( A , B ) Endothelial perfusion with RBCs at a sub-physiological level of shear stress (0.14 dyn/cm 2 ) gives rise to marked downregulation of nuclear HMGB1 expression (“low shear transfusion”), indicating severe necroptosis in the microvascular endothelium (*** p

    Journal: Scientific Reports

    Article Title: A microengineered model of RBC transfusion-induced pulmonary vascular injury

    doi: 10.1038/s41598-017-03597-w

    Figure Lengend Snippet: Effect of hemodynamic shear stress on transfusion-induced endothelial injury. ( A , B ) Endothelial perfusion with RBCs at a sub-physiological level of shear stress (0.14 dyn/cm 2 ) gives rise to marked downregulation of nuclear HMGB1 expression (“low shear transfusion”), indicating severe necroptosis in the microvascular endothelium (*** p

    Article Snippet: The concentration of HMGB1 in the collected samples was measured using a commercial HMGB1 detection kit following manufacturer’s protocol (Chondrex, Redmond, WA).

    Techniques: Expressing

    RBC transfusion-induced acute vascular injury. ( A ) Continuous RBC transfusion for 4 hours results in a loss of nuclear HMGB1 (red). Control data show HMGB1 expression in endothelial cells perfused with culture medium alone for the same period. Nuclei are stained with DAPI (blue). ( B ) Quantification of nuclear HMGB1 staining under control (without RBCs) and transfusion conditions (*** p

    Journal: Scientific Reports

    Article Title: A microengineered model of RBC transfusion-induced pulmonary vascular injury

    doi: 10.1038/s41598-017-03597-w

    Figure Lengend Snippet: RBC transfusion-induced acute vascular injury. ( A ) Continuous RBC transfusion for 4 hours results in a loss of nuclear HMGB1 (red). Control data show HMGB1 expression in endothelial cells perfused with culture medium alone for the same period. Nuclei are stained with DAPI (blue). ( B ) Quantification of nuclear HMGB1 staining under control (without RBCs) and transfusion conditions (*** p

    Article Snippet: The concentration of HMGB1 in the collected samples was measured using a commercial HMGB1 detection kit following manufacturer’s protocol (Chondrex, Redmond, WA).

    Techniques: Expressing, Staining

    Effect of mechanical stretch on transfusion-induced vascular injury. ( A ) The cell stretching system consists of grippers and motorized linear actuators that hold and stretch our cell culture microfluidic device in the lateral direction during perfusion experiments. ( B ) The entire platform can be mounted on a microscope stage for real-time monitoring and imaging of our model. ( C ) Uni-axial stretching of the microchannel by computer-controlled stretching system (Scale bar: 50 μm). The same microchannel was pseudo-colored at different levels of strain to show stretch-induced widening of the channel. ( D ) Quantification of extracellular HMGB1 measured in the fresh RBC units and perfusate from the control and transfusion groups in the presence and absence of cyclic stretch. Increases in the level of extracellular HMGB1 due to RBC transfusion are substantially higher when the endothelium is mechanically stretched at physiological strain (10%) and frequency (0.2 Hz) (*** p

    Journal: Scientific Reports

    Article Title: A microengineered model of RBC transfusion-induced pulmonary vascular injury

    doi: 10.1038/s41598-017-03597-w

    Figure Lengend Snippet: Effect of mechanical stretch on transfusion-induced vascular injury. ( A ) The cell stretching system consists of grippers and motorized linear actuators that hold and stretch our cell culture microfluidic device in the lateral direction during perfusion experiments. ( B ) The entire platform can be mounted on a microscope stage for real-time monitoring and imaging of our model. ( C ) Uni-axial stretching of the microchannel by computer-controlled stretching system (Scale bar: 50 μm). The same microchannel was pseudo-colored at different levels of strain to show stretch-induced widening of the channel. ( D ) Quantification of extracellular HMGB1 measured in the fresh RBC units and perfusate from the control and transfusion groups in the presence and absence of cyclic stretch. Increases in the level of extracellular HMGB1 due to RBC transfusion are substantially higher when the endothelium is mechanically stretched at physiological strain (10%) and frequency (0.2 Hz) (*** p

    Article Snippet: The concentration of HMGB1 in the collected samples was measured using a commercial HMGB1 detection kit following manufacturer’s protocol (Chondrex, Redmond, WA).

    Techniques: Cell Culture, Microscopy, Imaging

    Intra-peritoneal administration of mtDNA induces acute lung injury and systemic inflammation in a TLR9-dependent manner. WT mice were randomly assigned to a group pretreated with the TLR9 specific inhibitor ODN2088 or a control group. Mice in the ODN2088 were pretreated 1 h before mtDNA administration ( n = 16/group, 8 for BALF only). Acute lung injury was measured by HE staining (scale bar is 100 μm) ( A ) for lung injury score ( B ); BALF total protein concentration ( C ); and lung W/D ratio ( D ) were also analyzed. Systemic inflammation and circulating levels of IL-1β ( E ); IL-6 ( F ); and HMGB1 ( G ) were measured by ELISA. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Intra-Peritoneal Administration of Mitochondrial DNA Provokes Acute Lung Injury and Systemic Inflammation via Toll-Like Receptor 9

    doi: 10.3390/ijms17091425

    Figure Lengend Snippet: Intra-peritoneal administration of mtDNA induces acute lung injury and systemic inflammation in a TLR9-dependent manner. WT mice were randomly assigned to a group pretreated with the TLR9 specific inhibitor ODN2088 or a control group. Mice in the ODN2088 were pretreated 1 h before mtDNA administration ( n = 16/group, 8 for BALF only). Acute lung injury was measured by HE staining (scale bar is 100 μm) ( A ) for lung injury score ( B ); BALF total protein concentration ( C ); and lung W/D ratio ( D ) were also analyzed. Systemic inflammation and circulating levels of IL-1β ( E ); IL-6 ( F ); and HMGB1 ( G ) were measured by ELISA. * p

    Article Snippet: HMGB1 levels in all samples were determined using an HMGB1 Detection Kit (Chondrex Inc., Redmond, WA, USA), according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Staining, Protein Concentration, Enzyme-linked Immunosorbent Assay

    Intra-peritoneal administration of mtDNA induces acute lung injury and systemic inflammation in a TLR4-independent manner. WT and TLR4 KO mice were given NS, nDNA, and mtDNA administered intra-peritoneally ( n = 16/group, 8 for BALF only). Eight hours later, acute lung injury was measure by HE staining (scale bar is 100 μm) ( A ) for lung injury score ( B ); BALF total protein concentration ( C ); and lung W/D ratio ( D ) were also analyzed. Systemic inflammation and circulating levels of IL-1β ( E ); IL-6 ( F ); and HMGB1 ( G ) were measured by ELISA. + p > 0.05 versus WT/mtDNA group. Eight mice were used in each set and data are mean ± SEM of three separate experiments.

    Journal: International Journal of Molecular Sciences

    Article Title: Intra-Peritoneal Administration of Mitochondrial DNA Provokes Acute Lung Injury and Systemic Inflammation via Toll-Like Receptor 9

    doi: 10.3390/ijms17091425

    Figure Lengend Snippet: Intra-peritoneal administration of mtDNA induces acute lung injury and systemic inflammation in a TLR4-independent manner. WT and TLR4 KO mice were given NS, nDNA, and mtDNA administered intra-peritoneally ( n = 16/group, 8 for BALF only). Eight hours later, acute lung injury was measure by HE staining (scale bar is 100 μm) ( A ) for lung injury score ( B ); BALF total protein concentration ( C ); and lung W/D ratio ( D ) were also analyzed. Systemic inflammation and circulating levels of IL-1β ( E ); IL-6 ( F ); and HMGB1 ( G ) were measured by ELISA. + p > 0.05 versus WT/mtDNA group. Eight mice were used in each set and data are mean ± SEM of three separate experiments.

    Article Snippet: HMGB1 levels in all samples were determined using an HMGB1 Detection Kit (Chondrex Inc., Redmond, WA, USA), according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Staining, Protein Concentration, Enzyme-linked Immunosorbent Assay

    Intra-peritoneal administration of mtDNA leads to systemic inflammation. WT mice were treated with PBS, nuclear DNA (nDNA), or mtDNA via intra-peritoneal injection for 2, 8, and 24 h. Systemic inflammation and circulating levels of IL-1β ( A ); IL-6 ( B ); and HMGB1 ( C ) were measured by ELISA. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Intra-Peritoneal Administration of Mitochondrial DNA Provokes Acute Lung Injury and Systemic Inflammation via Toll-Like Receptor 9

    doi: 10.3390/ijms17091425

    Figure Lengend Snippet: Intra-peritoneal administration of mtDNA leads to systemic inflammation. WT mice were treated with PBS, nuclear DNA (nDNA), or mtDNA via intra-peritoneal injection for 2, 8, and 24 h. Systemic inflammation and circulating levels of IL-1β ( A ); IL-6 ( B ); and HMGB1 ( C ) were measured by ELISA. * p

    Article Snippet: HMGB1 levels in all samples were determined using an HMGB1 Detection Kit (Chondrex Inc., Redmond, WA, USA), according to the manufacturer’s instructions.

    Techniques: Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay

    HMGB1 released by three cell lines 24 h after treatment with either NPS, or DOX or MTX. The number of pulses applied to achieve the indicated J/mL for all cell lines are indicated above the MCA205 plot. Significant difference from untreated controls by one-way ANOVA with between group analysis performed using the Dunnett’s test. * p

    Journal: Journal for Immunotherapy of Cancer

    Article Title: Nano-Pulse Stimulation is a physical modality that can trigger immunogenic tumor cell death

    doi: 10.1186/s40425-017-0234-5

    Figure Lengend Snippet: HMGB1 released by three cell lines 24 h after treatment with either NPS, or DOX or MTX. The number of pulses applied to achieve the indicated J/mL for all cell lines are indicated above the MCA205 plot. Significant difference from untreated controls by one-way ANOVA with between group analysis performed using the Dunnett’s test. * p

    Article Snippet: HMGB1 ELISA HMGB1 release was measured with the HMGB1 Detection Kit (Chondrex, Inc.; Redmond, WA).

    Techniques: