Journal: Journal of Innate Immunity
Article Title: TGF-β1 Impairs Vitamin D-Induced and Constitutive Airway Epithelial Host Defense Mechanisms
doi: 10.1159/000497415
Figure Lengend Snippet: Antibodies used for immunofluorescence staining
Article Snippet: Finally, cells were mounted in ProLong TM Gold Antifade Mountant (Thermo Fisher scientific) and images were acquired using a TCS SP5 Confocal Laser Scanning Microscope (Leica Microsystems B.V., Eindhoven, The Netherlands) and LAS AF Lite software (Leica Microsystems B.V.). table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Antibody Supplier Catalog number Species Antibody dilution CYP24A1 Sigma HPA022261 Rabbit 1/100 pIgR R&D systems MAB27171 Mouse 1/50 P63 Abcam ab124762 Rabbit 1/100 P63 Leica NCL-P63 Mouse 1/100 sPLUNC Hycult biotech HM2314 Mouse 1/100 SLPI Hycult biotech HM2037 Mouse 1/100 Mucin 5AC Labvision neomarkers MS-145-P1 Mouse 1/1,000 CC16 Hycult biotech HM2178 Mouse 1/50 Open in a separate window pIgR, polymeric immunoglobulin receptor; SLPI, secretory leukocyte protease inhibitor.
Techniques: Immunofluorescence