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Millipore hla dr2
( A ) SDS-PAGE analysis (12%) of detergent solubilized <t>HLA-DR2</t> (DRB5*0101/DRB1*1501; left) or HLA-DR4 (DRB1*0401; right) where 5–8×10 9 of the Epstein Barr virus (EBV)-transformed B cell line HTC-LAN and BSM were lysed, respectively, and their affinity was purified using the immobilized monoclonal antibody L243. After concentration on a 30-kDa membrane, 670–700 g of the human leukocyte antigen (HLA)-II were obtained and stored in phosphate buffered saline at a concentration of 0.5 mg/mL. The purity of HLA-DR2 and HLA-DR4 were about 85%. ( B ) Competition binding of the MBP 82–98 peptide analogues in 10-fold, 20-fold, and 50-fold excess of AMCA-labeled MBP 83–99 , which is specific to the HLA-DR2b chain, was used as the peptide competitor, and ( C ) AMCA-labeled HA 306–318 , which is specific to the HLA-DR4, was used as the peptide competitor. All of the experiments were in triplicates, where ** p
Hla Dr2, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Design of Linear and Cyclic Mutant Analogues of Dirucotide Peptide (MBP82–98) against Multiple Sclerosis: Conformational and Binding Studies to MHC Class II"

Article Title: Design of Linear and Cyclic Mutant Analogues of Dirucotide Peptide (MBP82–98) against Multiple Sclerosis: Conformational and Binding Studies to MHC Class II

Journal: Brain Sciences

doi: 10.3390/brainsci8120213

( A ) SDS-PAGE analysis (12%) of detergent solubilized HLA-DR2 (DRB5*0101/DRB1*1501; left) or HLA-DR4 (DRB1*0401; right) where 5–8×10 9 of the Epstein Barr virus (EBV)-transformed B cell line HTC-LAN and BSM were lysed, respectively, and their affinity was purified using the immobilized monoclonal antibody L243. After concentration on a 30-kDa membrane, 670–700 g of the human leukocyte antigen (HLA)-II were obtained and stored in phosphate buffered saline at a concentration of 0.5 mg/mL. The purity of HLA-DR2 and HLA-DR4 were about 85%. ( B ) Competition binding of the MBP 82–98 peptide analogues in 10-fold, 20-fold, and 50-fold excess of AMCA-labeled MBP 83–99 , which is specific to the HLA-DR2b chain, was used as the peptide competitor, and ( C ) AMCA-labeled HA 306–318 , which is specific to the HLA-DR4, was used as the peptide competitor. All of the experiments were in triplicates, where ** p
Figure Legend Snippet: ( A ) SDS-PAGE analysis (12%) of detergent solubilized HLA-DR2 (DRB5*0101/DRB1*1501; left) or HLA-DR4 (DRB1*0401; right) where 5–8×10 9 of the Epstein Barr virus (EBV)-transformed B cell line HTC-LAN and BSM were lysed, respectively, and their affinity was purified using the immobilized monoclonal antibody L243. After concentration on a 30-kDa membrane, 670–700 g of the human leukocyte antigen (HLA)-II were obtained and stored in phosphate buffered saline at a concentration of 0.5 mg/mL. The purity of HLA-DR2 and HLA-DR4 were about 85%. ( B ) Competition binding of the MBP 82–98 peptide analogues in 10-fold, 20-fold, and 50-fold excess of AMCA-labeled MBP 83–99 , which is specific to the HLA-DR2b chain, was used as the peptide competitor, and ( C ) AMCA-labeled HA 306–318 , which is specific to the HLA-DR4, was used as the peptide competitor. All of the experiments were in triplicates, where ** p

Techniques Used: SDS Page, Transformation Assay, Purification, Concentration Assay, Binding Assay, Labeling

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Article Title: Design of Linear and Cyclic Mutant Analogues of Dirucotide Peptide (MBP82–98) against Multiple Sclerosis: Conformational and Binding Studies to MHC Class II
Article Snippet: .. Solubilized HLA-DR2 (0.66 mM) and HLA-DR4 (0.11 mM) were incubated for 48 h at 37 °C with MBP83–99 -(AMCA)-labeled peptide or N-terminally 7-amino-4-methylcoumarin-3-acetic acid (AMCA)-labeled influenza matrix peptide (AMCA-HA306–318 -peptide), dissolved in 150 mM of sodium phosphate, pH 6.0, containing 15% acetonitrile, and 0.1% Zwittergent-12 (Calbiochem, San Diego, CA, USA). ..

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    Millipore hla dr2
    ( A ) SDS-PAGE analysis (12%) of detergent solubilized <t>HLA-DR2</t> (DRB5*0101/DRB1*1501; left) or HLA-DR4 (DRB1*0401; right) where 5–8×10 9 of the Epstein Barr virus (EBV)-transformed B cell line HTC-LAN and BSM were lysed, respectively, and their affinity was purified using the immobilized monoclonal antibody L243. After concentration on a 30-kDa membrane, 670–700 g of the human leukocyte antigen (HLA)-II were obtained and stored in phosphate buffered saline at a concentration of 0.5 mg/mL. The purity of HLA-DR2 and HLA-DR4 were about 85%. ( B ) Competition binding of the MBP 82–98 peptide analogues in 10-fold, 20-fold, and 50-fold excess of AMCA-labeled MBP 83–99 , which is specific to the HLA-DR2b chain, was used as the peptide competitor, and ( C ) AMCA-labeled HA 306–318 , which is specific to the HLA-DR4, was used as the peptide competitor. All of the experiments were in triplicates, where ** p
    Hla Dr2, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hla dr2/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hla dr2 - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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    ( A ) SDS-PAGE analysis (12%) of detergent solubilized HLA-DR2 (DRB5*0101/DRB1*1501; left) or HLA-DR4 (DRB1*0401; right) where 5–8×10 9 of the Epstein Barr virus (EBV)-transformed B cell line HTC-LAN and BSM were lysed, respectively, and their affinity was purified using the immobilized monoclonal antibody L243. After concentration on a 30-kDa membrane, 670–700 g of the human leukocyte antigen (HLA)-II were obtained and stored in phosphate buffered saline at a concentration of 0.5 mg/mL. The purity of HLA-DR2 and HLA-DR4 were about 85%. ( B ) Competition binding of the MBP 82–98 peptide analogues in 10-fold, 20-fold, and 50-fold excess of AMCA-labeled MBP 83–99 , which is specific to the HLA-DR2b chain, was used as the peptide competitor, and ( C ) AMCA-labeled HA 306–318 , which is specific to the HLA-DR4, was used as the peptide competitor. All of the experiments were in triplicates, where ** p

    Journal: Brain Sciences

    Article Title: Design of Linear and Cyclic Mutant Analogues of Dirucotide Peptide (MBP82–98) against Multiple Sclerosis: Conformational and Binding Studies to MHC Class II

    doi: 10.3390/brainsci8120213

    Figure Lengend Snippet: ( A ) SDS-PAGE analysis (12%) of detergent solubilized HLA-DR2 (DRB5*0101/DRB1*1501; left) or HLA-DR4 (DRB1*0401; right) where 5–8×10 9 of the Epstein Barr virus (EBV)-transformed B cell line HTC-LAN and BSM were lysed, respectively, and their affinity was purified using the immobilized monoclonal antibody L243. After concentration on a 30-kDa membrane, 670–700 g of the human leukocyte antigen (HLA)-II were obtained and stored in phosphate buffered saline at a concentration of 0.5 mg/mL. The purity of HLA-DR2 and HLA-DR4 were about 85%. ( B ) Competition binding of the MBP 82–98 peptide analogues in 10-fold, 20-fold, and 50-fold excess of AMCA-labeled MBP 83–99 , which is specific to the HLA-DR2b chain, was used as the peptide competitor, and ( C ) AMCA-labeled HA 306–318 , which is specific to the HLA-DR4, was used as the peptide competitor. All of the experiments were in triplicates, where ** p

    Article Snippet: Solubilized HLA-DR2 (0.66 mM) and HLA-DR4 (0.11 mM) were incubated for 48 h at 37 °C with MBP83–99 -(AMCA)-labeled peptide or N-terminally 7-amino-4-methylcoumarin-3-acetic acid (AMCA)-labeled influenza matrix peptide (AMCA-HA306–318 -peptide), dissolved in 150 mM of sodium phosphate, pH 6.0, containing 15% acetonitrile, and 0.1% Zwittergent-12 (Calbiochem, San Diego, CA, USA).

    Techniques: SDS Page, Transformation Assay, Purification, Concentration Assay, Binding Assay, Labeling