Structured Review

OriGene hkat open reading frame
<t>Hkat</t> tissue expression pattern and subcellular localization. A) Hkat is expression in both white adipose tissue and brown adipose tissue, but is highest expressed in heart and kidney. B) Hkat is localized to the plasma membrane. Fluorescence imaging of HEK 293 cells transfected with plasmids encoding B) LCK-tRFP and C) <t>Hkat-GFP</t> and D) Merged picture.
Hkat Open Reading Frame, supplied by OriGene, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hkat open reading frame/product/OriGene
Average 85 stars, based on 3 article reviews
Price from $9.99 to $1999.99
hkat open reading frame - by Bioz Stars, 2020-09
85/100 stars

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1) Product Images from "Hkat, a novel nutritionally regulated transmembrane protein in adipose tissues"

Article Title: Hkat, a novel nutritionally regulated transmembrane protein in adipose tissues

Journal: Scientific Reports

doi: 10.1038/srep00825

Hkat tissue expression pattern and subcellular localization. A) Hkat is expression in both white adipose tissue and brown adipose tissue, but is highest expressed in heart and kidney. B) Hkat is localized to the plasma membrane. Fluorescence imaging of HEK 293 cells transfected with plasmids encoding B) LCK-tRFP and C) Hkat-GFP and D) Merged picture.
Figure Legend Snippet: Hkat tissue expression pattern and subcellular localization. A) Hkat is expression in both white adipose tissue and brown adipose tissue, but is highest expressed in heart and kidney. B) Hkat is localized to the plasma membrane. Fluorescence imaging of HEK 293 cells transfected with plasmids encoding B) LCK-tRFP and C) Hkat-GFP and D) Merged picture.

Techniques Used: Expressing, Fluorescence, Imaging, Transfection

Related Articles

Clone Assay:

Article Title: Hkat, a novel nutritionally regulated transmembrane protein in adipose tissues
Article Snippet: .. Imaging To examine Hkat subcellular localization, fusion protein of Hkat-GFP was made by cloning the Hkat open reading frame (Origene, MD) into the pCMV6-AC-GFP vector. .. In HEK293 cells, the Hkat-GFP vector was cotransfected with a pCMV6 vector that encodes a fusion protein of LCK (lymphocyte-specific protein tyrosine kinase) and red fluorescence protein (Origene, MD) using Lipofectamine 2000 (Invitrogen, Grand Island, NY).

Imaging:

Article Title: Hkat, a novel nutritionally regulated transmembrane protein in adipose tissues
Article Snippet: .. Imaging To examine Hkat subcellular localization, fusion protein of Hkat-GFP was made by cloning the Hkat open reading frame (Origene, MD) into the pCMV6-AC-GFP vector. .. In HEK293 cells, the Hkat-GFP vector was cotransfected with a pCMV6 vector that encodes a fusion protein of LCK (lymphocyte-specific protein tyrosine kinase) and red fluorescence protein (Origene, MD) using Lipofectamine 2000 (Invitrogen, Grand Island, NY).

Plasmid Preparation:

Article Title: Hkat, a novel nutritionally regulated transmembrane protein in adipose tissues
Article Snippet: .. Imaging To examine Hkat subcellular localization, fusion protein of Hkat-GFP was made by cloning the Hkat open reading frame (Origene, MD) into the pCMV6-AC-GFP vector. .. In HEK293 cells, the Hkat-GFP vector was cotransfected with a pCMV6 vector that encodes a fusion protein of LCK (lymphocyte-specific protein tyrosine kinase) and red fluorescence protein (Origene, MD) using Lipofectamine 2000 (Invitrogen, Grand Island, NY).

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    OriGene hkat open reading frame
    <t>Hkat</t> tissue expression pattern and subcellular localization. A) Hkat is expression in both white adipose tissue and brown adipose tissue, but is highest expressed in heart and kidney. B) Hkat is localized to the plasma membrane. Fluorescence imaging of HEK 293 cells transfected with plasmids encoding B) LCK-tRFP and C) <t>Hkat-GFP</t> and D) Merged picture.
    Hkat Open Reading Frame, supplied by OriGene, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hkat open reading frame/product/OriGene
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hkat open reading frame - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    Hkat tissue expression pattern and subcellular localization. A) Hkat is expression in both white adipose tissue and brown adipose tissue, but is highest expressed in heart and kidney. B) Hkat is localized to the plasma membrane. Fluorescence imaging of HEK 293 cells transfected with plasmids encoding B) LCK-tRFP and C) Hkat-GFP and D) Merged picture.

    Journal: Scientific Reports

    Article Title: Hkat, a novel nutritionally regulated transmembrane protein in adipose tissues

    doi: 10.1038/srep00825

    Figure Lengend Snippet: Hkat tissue expression pattern and subcellular localization. A) Hkat is expression in both white adipose tissue and brown adipose tissue, but is highest expressed in heart and kidney. B) Hkat is localized to the plasma membrane. Fluorescence imaging of HEK 293 cells transfected with plasmids encoding B) LCK-tRFP and C) Hkat-GFP and D) Merged picture.

    Article Snippet: Imaging To examine Hkat subcellular localization, fusion protein of Hkat-GFP was made by cloning the Hkat open reading frame (Origene, MD) into the pCMV6-AC-GFP vector.

    Techniques: Expressing, Fluorescence, Imaging, Transfection