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TaKaRa hiv primer specific ngs platform
Reverse transcription temperature optimization. The trimmed <t>NGS</t> reads from <t>HIV-SMART</t> libraries prepared at the indicated reverse transcription temperature (42°C, 47°C, or 50°C) were mapped to the HXB2 reference genome. (A, B) The genome coverage (A) and percentage of HIV reads (B) for this alignment were calculated by use of the CLC Bio software. (C) The viral load for each sample tested is plotted on a log scale. (D) The genome coverage plots for each position of the genome are shown for NGSID 12, which showed a trend representative of the trends seen in all other samples tested. y axis, number of reads; x axis, nucleotide position in the genome sequence.
Hiv Primer Specific Ngs Platform, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hiv primer specific ngs platform/product/TaKaRa
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
hiv primer specific ngs platform - by Bioz Stars, 2020-08
91/100 stars

Related Products / Commonly Used Together

rna template
hiv-switching mechanism

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1) Product Images from "Sensitive Next-Generation Sequencing Method Reveals Deep Genetic Diversity of HIV-1 in the Democratic Republic of the Congo"

Article Title: Sensitive Next-Generation Sequencing Method Reveals Deep Genetic Diversity of HIV-1 in the Democratic Republic of the Congo

Journal: Journal of Virology

doi: 10.1128/JVI.01841-16

Reverse transcription temperature optimization. The trimmed NGS reads from HIV-SMART libraries prepared at the indicated reverse transcription temperature (42°C, 47°C, or 50°C) were mapped to the HXB2 reference genome. (A, B) The genome coverage (A) and percentage of HIV reads (B) for this alignment were calculated by use of the CLC Bio software. (C) The viral load for each sample tested is plotted on a log scale. (D) The genome coverage plots for each position of the genome are shown for NGSID 12, which showed a trend representative of the trends seen in all other samples tested. y axis, number of reads; x axis, nucleotide position in the genome sequence.
Figure Legend Snippet: Reverse transcription temperature optimization. The trimmed NGS reads from HIV-SMART libraries prepared at the indicated reverse transcription temperature (42°C, 47°C, or 50°C) were mapped to the HXB2 reference genome. (A, B) The genome coverage (A) and percentage of HIV reads (B) for this alignment were calculated by use of the CLC Bio software. (C) The viral load for each sample tested is plotted on a log scale. (D) The genome coverage plots for each position of the genome are shown for NGSID 12, which showed a trend representative of the trends seen in all other samples tested. y axis, number of reads; x axis, nucleotide position in the genome sequence.

Techniques Used: Next-Generation Sequencing, Software, Sequencing

Library preparation optimization. The trimmed NGS reads from HIV-SMART libraries were prepared by protocols A to E (A, the standard protocol; B, a protocol with the Pico SMART cDNA kit; C, a protocol with a nucleic acid concentrator; D, a protocol with a sizing column; E, a protocol that followed the nucleic concentration protocol [Conc.] in protocol C with reverse transcription at 47°C) and mapped to the HXB2 reference genome. (A, B) The genome coverage (A) and percentage of HIV reads (B) were calculated for this alignment by the use of CLC Bio software. (C) The viral load for each sample tested is plotted on a log scale. (D) The genome coverage plots for each position of the genome are shown for isolate NGSID 4, which showed a trend representative of the trends seen for all other isolates tested. y axis, number of reads; x axis, nucleotide position in the genome sequence.
Figure Legend Snippet: Library preparation optimization. The trimmed NGS reads from HIV-SMART libraries were prepared by protocols A to E (A, the standard protocol; B, a protocol with the Pico SMART cDNA kit; C, a protocol with a nucleic acid concentrator; D, a protocol with a sizing column; E, a protocol that followed the nucleic concentration protocol [Conc.] in protocol C with reverse transcription at 47°C) and mapped to the HXB2 reference genome. (A, B) The genome coverage (A) and percentage of HIV reads (B) were calculated for this alignment by the use of CLC Bio software. (C) The viral load for each sample tested is plotted on a log scale. (D) The genome coverage plots for each position of the genome are shown for isolate NGSID 4, which showed a trend representative of the trends seen for all other isolates tested. y axis, number of reads; x axis, nucleotide position in the genome sequence.

Techniques Used: Next-Generation Sequencing, Concentration Assay, Software, Sequencing

Related Articles

Next-Generation Sequencing:

Article Title: Sensitive Next-Generation Sequencing Method Reveals Deep Genetic Diversity of HIV-1 in the Democratic Republic of the Congo
Article Snippet: .. Recently, we developed a new HIV primer-specific NGS platform, called HIV-switching mechanism at the 5′ end of the RNA template (SMART; Clontech) ( ) to obtain complete genomes from HIV-1 groups M, N, O, and P as well as HIV-2 isolates. .. This method utilizes a set of 6 pan-HIV-specific primers fused to the SMART sequence to create libraries for NGS on the Illumina MiSeq instrument ( ).

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    TaKaRa hiv primer specific ngs platform
    Reverse transcription temperature optimization. The trimmed <t>NGS</t> reads from <t>HIV-SMART</t> libraries prepared at the indicated reverse transcription temperature (42°C, 47°C, or 50°C) were mapped to the HXB2 reference genome. (A, B) The genome coverage (A) and percentage of HIV reads (B) for this alignment were calculated by use of the CLC Bio software. (C) The viral load for each sample tested is plotted on a log scale. (D) The genome coverage plots for each position of the genome are shown for NGSID 12, which showed a trend representative of the trends seen in all other samples tested. y axis, number of reads; x axis, nucleotide position in the genome sequence.
    Hiv Primer Specific Ngs Platform, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv primer specific ngs platform/product/TaKaRa
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv primer specific ngs platform - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

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    Reverse transcription temperature optimization. The trimmed NGS reads from HIV-SMART libraries prepared at the indicated reverse transcription temperature (42°C, 47°C, or 50°C) were mapped to the HXB2 reference genome. (A, B) The genome coverage (A) and percentage of HIV reads (B) for this alignment were calculated by use of the CLC Bio software. (C) The viral load for each sample tested is plotted on a log scale. (D) The genome coverage plots for each position of the genome are shown for NGSID 12, which showed a trend representative of the trends seen in all other samples tested. y axis, number of reads; x axis, nucleotide position in the genome sequence.

    Journal: Journal of Virology

    Article Title: Sensitive Next-Generation Sequencing Method Reveals Deep Genetic Diversity of HIV-1 in the Democratic Republic of the Congo

    doi: 10.1128/JVI.01841-16

    Figure Lengend Snippet: Reverse transcription temperature optimization. The trimmed NGS reads from HIV-SMART libraries prepared at the indicated reverse transcription temperature (42°C, 47°C, or 50°C) were mapped to the HXB2 reference genome. (A, B) The genome coverage (A) and percentage of HIV reads (B) for this alignment were calculated by use of the CLC Bio software. (C) The viral load for each sample tested is plotted on a log scale. (D) The genome coverage plots for each position of the genome are shown for NGSID 12, which showed a trend representative of the trends seen in all other samples tested. y axis, number of reads; x axis, nucleotide position in the genome sequence.

    Article Snippet: Recently, we developed a new HIV primer-specific NGS platform, called HIV-switching mechanism at the 5′ end of the RNA template (SMART; Clontech) ( ) to obtain complete genomes from HIV-1 groups M, N, O, and P as well as HIV-2 isolates.

    Techniques: Next-Generation Sequencing, Software, Sequencing

    Library preparation optimization. The trimmed NGS reads from HIV-SMART libraries were prepared by protocols A to E (A, the standard protocol; B, a protocol with the Pico SMART cDNA kit; C, a protocol with a nucleic acid concentrator; D, a protocol with a sizing column; E, a protocol that followed the nucleic concentration protocol [Conc.] in protocol C with reverse transcription at 47°C) and mapped to the HXB2 reference genome. (A, B) The genome coverage (A) and percentage of HIV reads (B) were calculated for this alignment by the use of CLC Bio software. (C) The viral load for each sample tested is plotted on a log scale. (D) The genome coverage plots for each position of the genome are shown for isolate NGSID 4, which showed a trend representative of the trends seen for all other isolates tested. y axis, number of reads; x axis, nucleotide position in the genome sequence.

    Journal: Journal of Virology

    Article Title: Sensitive Next-Generation Sequencing Method Reveals Deep Genetic Diversity of HIV-1 in the Democratic Republic of the Congo

    doi: 10.1128/JVI.01841-16

    Figure Lengend Snippet: Library preparation optimization. The trimmed NGS reads from HIV-SMART libraries were prepared by protocols A to E (A, the standard protocol; B, a protocol with the Pico SMART cDNA kit; C, a protocol with a nucleic acid concentrator; D, a protocol with a sizing column; E, a protocol that followed the nucleic concentration protocol [Conc.] in protocol C with reverse transcription at 47°C) and mapped to the HXB2 reference genome. (A, B) The genome coverage (A) and percentage of HIV reads (B) were calculated for this alignment by the use of CLC Bio software. (C) The viral load for each sample tested is plotted on a log scale. (D) The genome coverage plots for each position of the genome are shown for isolate NGSID 4, which showed a trend representative of the trends seen for all other isolates tested. y axis, number of reads; x axis, nucleotide position in the genome sequence.

    Article Snippet: Recently, we developed a new HIV primer-specific NGS platform, called HIV-switching mechanism at the 5′ end of the RNA template (SMART; Clontech) ( ) to obtain complete genomes from HIV-1 groups M, N, O, and P as well as HIV-2 isolates.

    Techniques: Next-Generation Sequencing, Concentration Assay, Software, Sequencing