hiv 1 envelope  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hiv 1 envelope
    Effects of integration inhibitor L-731,988. (A) Complete inhibition of virus production by integrase inhibitor L-731,988. Activated CD4 + T cells were infected with the X4-Env-pseudotyped <t>HIV-1</t> reporter virus in the absence (open circles) or the
    Hiv 1 Envelope, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv 1 envelope/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv 1 envelope - by Bioz Stars, 2022-09
    93/100 stars

    Images

    1) Product Images from "Kinetics of Human Immunodeficiency Virus Type 1 Decay following Entry into Resting CD4+ T Cells"

    Article Title: Kinetics of Human Immunodeficiency Virus Type 1 Decay following Entry into Resting CD4+ T Cells

    Journal:

    doi: 10.1128/JVI.79.4.2199-2210.2005

    Effects of integration inhibitor L-731,988. (A) Complete inhibition of virus production by integrase inhibitor L-731,988. Activated CD4 + T cells were infected with the X4-Env-pseudotyped HIV-1 reporter virus in the absence (open circles) or the
    Figure Legend Snippet: Effects of integration inhibitor L-731,988. (A) Complete inhibition of virus production by integrase inhibitor L-731,988. Activated CD4 + T cells were infected with the X4-Env-pseudotyped HIV-1 reporter virus in the absence (open circles) or the

    Techniques Used: Inhibition, Infection

    Decay of HIV-1 in resting CD4 + T cells. (A) Schematic representation of experimental strategy for studying HIV-1 decay in resting CD4 + T cells. Resting CD4 + T cells were infected, and 4 h later the cells were treated with the fusion
    Figure Legend Snippet: Decay of HIV-1 in resting CD4 + T cells. (A) Schematic representation of experimental strategy for studying HIV-1 decay in resting CD4 + T cells. Resting CD4 + T cells were infected, and 4 h later the cells were treated with the fusion

    Techniques Used: Infection

    In vitro model of acutely infected resting CD4 + T cells. (A) Recombinant HIV-1 vector used for infection of resting CD4 + T cells. Enhanced GFP was inserted in frame into the HIV-1 env gene in place of nucleotides 6348 to 7251 to produce
    Figure Legend Snippet: In vitro model of acutely infected resting CD4 + T cells. (A) Recombinant HIV-1 vector used for infection of resting CD4 + T cells. Enhanced GFP was inserted in frame into the HIV-1 env gene in place of nucleotides 6348 to 7251 to produce

    Techniques Used: In Vitro, Infection, Recombinant, Plasmid Preparation

    Decay of HIV-1 before the completion of reverse transcription. (A) Experimental strategy for measuring the kinetics of HIV-1 decay in relation to the completion of reverse transcription. In these experiments, 3TC was added 4 h prior to activation and
    Figure Legend Snippet: Decay of HIV-1 before the completion of reverse transcription. (A) Experimental strategy for measuring the kinetics of HIV-1 decay in relation to the completion of reverse transcription. In these experiments, 3TC was added 4 h prior to activation and

    Techniques Used: Activation Assay

    Decay of integration-competent HIV-1 in resting CD4 + T cells. (A) Experimental strategy for monitoring the functional decay of full-length, integration-competent viral DNA by blocking integration with L-731,988 upon T-cell activation. 3TC was
    Figure Legend Snippet: Decay of integration-competent HIV-1 in resting CD4 + T cells. (A) Experimental strategy for monitoring the functional decay of full-length, integration-competent viral DNA by blocking integration with L-731,988 upon T-cell activation. 3TC was

    Techniques Used: Functional Assay, Blocking Assay, Activation Assay

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    Thermo Fisher cd16 functional grade monoclonal antibody
    HIV-specific responses and immune response capability of the case at 9.5 years of age. a Detection of HIV-specific antibodies at 9.5 years of age by western blot. The case antibody profile is compared with controls that are a high positive, low positive and HIV-negative. HIV proteins corresponding to bands in the blots are shown in the left grey-shaded block; the case profile was positive for the core proteins indicated in pink. b Quantitation of HIV-specific antibodies by multiplex bead array for all isotypes and subclasses (indicated on the left side—IgG1, IgG2, IgG3, IgG4, IgM, IgA1, IgA2) against gp41, Gag, RT, gp120, Nef, Tat, Vpu, peptide Vpu9 and V1V2 scaffold antigens (indicated at the top). Results are expressed as mean fluorescence intensities (MFI)), the colour key shows ranges of MFI according to colour intensity (the darker the more HIV-specific antibody detected). A result is considered positive if above the cut-off (mean ± 3 SD) determined from eight adult uninfected controls. Vpu9 amino acid sequence: STMVDMGHLRLLDVNDL. c Proportions of natural killer (NK) cells that respond to <t>anti-CD16</t> antibody, and CD4+ and CD8+ T cells that respond to staphylococcal enterotoxin B (SEB) in a whole blood intracellular cytokine (ICC) assay that measures induction of interferon-γ (IFN-γ) and interleukin-2 (IL-2). HIV-uninfected adult reference values for comparison ( n = 21; median % and range)—natural killer (NK) anti-CD16%: 37.92 (12–67.6), CD4 SEB%: 6.04 (0.25–11.91), CD8 SEB%: 5.82 (0.18–18.94). d A weak positive CD4+ T cell response to Gag (0.116%) in the absence of a detectable CD8+ T cell response to Gag (
    Cd16 Functional Grade Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd16 functional grade monoclonal antibody/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
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    93
    Thermo Fisher hiv 1 envelope
    Effects of integration inhibitor L-731,988. (A) Complete inhibition of virus production by integrase inhibitor L-731,988. Activated CD4 + T cells were infected with the X4-Env-pseudotyped <t>HIV-1</t> reporter virus in the absence (open circles) or the
    Hiv 1 Envelope, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv 1 envelope/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv 1 envelope - by Bioz Stars, 2022-09
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      Buy from Supplier

    96
    Thermo Fisher hiv 1
    Viability of IPCs cultured with <t>HIV-1</t> or HSV. Viable cells were counted by trypan blue staining. Percentages of viable IPCs cultured under different conditions were indicated. H.I., heat inactivated.
    Hiv 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv 1/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    94
    Thermo Fisher hiv 1 tat monoclonal antibody
    (A) Red represents MT2 cells, blue represents TZM-bl cells, black represents H9 cells, and green represents 293T cells. The X-axis is the time of infection. The Y-axis represents log 10 of the <t>HIV-1</t> pol load, and the units are indicated in the title of the Y-axis. Connecting curves are indicated. (B) Red represents MT2 cells, blue represents TZM-bl cells, black represents H9 cells, and green represents 293T cells. The X-axis is the time of infection. The Y-axis shows the fold increase in the transcription level in the HERV-K env region calculated by the 2 −ΔΔCT method. Standard errors for triplicate experiments are indicated. * P
    Hiv 1 Tat Monoclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv 1 tat monoclonal antibody/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv 1 tat monoclonal antibody - by Bioz Stars, 2022-09
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      Buy from Supplier

    Image Search Results


    HIV-specific responses and immune response capability of the case at 9.5 years of age. a Detection of HIV-specific antibodies at 9.5 years of age by western blot. The case antibody profile is compared with controls that are a high positive, low positive and HIV-negative. HIV proteins corresponding to bands in the blots are shown in the left grey-shaded block; the case profile was positive for the core proteins indicated in pink. b Quantitation of HIV-specific antibodies by multiplex bead array for all isotypes and subclasses (indicated on the left side—IgG1, IgG2, IgG3, IgG4, IgM, IgA1, IgA2) against gp41, Gag, RT, gp120, Nef, Tat, Vpu, peptide Vpu9 and V1V2 scaffold antigens (indicated at the top). Results are expressed as mean fluorescence intensities (MFI)), the colour key shows ranges of MFI according to colour intensity (the darker the more HIV-specific antibody detected). A result is considered positive if above the cut-off (mean ± 3 SD) determined from eight adult uninfected controls. Vpu9 amino acid sequence: STMVDMGHLRLLDVNDL. c Proportions of natural killer (NK) cells that respond to anti-CD16 antibody, and CD4+ and CD8+ T cells that respond to staphylococcal enterotoxin B (SEB) in a whole blood intracellular cytokine (ICC) assay that measures induction of interferon-γ (IFN-γ) and interleukin-2 (IL-2). HIV-uninfected adult reference values for comparison ( n = 21; median % and range)—natural killer (NK) anti-CD16%: 37.92 (12–67.6), CD4 SEB%: 6.04 (0.25–11.91), CD8 SEB%: 5.82 (0.18–18.94). d A weak positive CD4+ T cell response to Gag (0.116%) in the absence of a detectable CD8+ T cell response to Gag (

    Journal: Nature Communications

    Article Title: A child with perinatal HIV infection and long-term sustained virological control following antiretroviral treatment cessation

    doi: 10.1038/s41467-019-08311-0

    Figure Lengend Snippet: HIV-specific responses and immune response capability of the case at 9.5 years of age. a Detection of HIV-specific antibodies at 9.5 years of age by western blot. The case antibody profile is compared with controls that are a high positive, low positive and HIV-negative. HIV proteins corresponding to bands in the blots are shown in the left grey-shaded block; the case profile was positive for the core proteins indicated in pink. b Quantitation of HIV-specific antibodies by multiplex bead array for all isotypes and subclasses (indicated on the left side—IgG1, IgG2, IgG3, IgG4, IgM, IgA1, IgA2) against gp41, Gag, RT, gp120, Nef, Tat, Vpu, peptide Vpu9 and V1V2 scaffold antigens (indicated at the top). Results are expressed as mean fluorescence intensities (MFI)), the colour key shows ranges of MFI according to colour intensity (the darker the more HIV-specific antibody detected). A result is considered positive if above the cut-off (mean ± 3 SD) determined from eight adult uninfected controls. Vpu9 amino acid sequence: STMVDMGHLRLLDVNDL. c Proportions of natural killer (NK) cells that respond to anti-CD16 antibody, and CD4+ and CD8+ T cells that respond to staphylococcal enterotoxin B (SEB) in a whole blood intracellular cytokine (ICC) assay that measures induction of interferon-γ (IFN-γ) and interleukin-2 (IL-2). HIV-uninfected adult reference values for comparison ( n = 21; median % and range)—natural killer (NK) anti-CD16%: 37.92 (12–67.6), CD4 SEB%: 6.04 (0.25–11.91), CD8 SEB%: 5.82 (0.18–18.94). d A weak positive CD4+ T cell response to Gag (0.116%) in the absence of a detectable CD8+ T cell response to Gag (

    Article Snippet: Positive controls were stimulated with 1 µg/mL SEB (Cat. No. S4881, Sigma-Aldrich) for T cells or with 5 ng mL−1 anti-CD16 antibody (Clone eBioCB16, Cat. No. 16-0168-85, eBioscience, San Diego, CA, USA) for NK cells.

    Techniques: Western Blot, Blocking Assay, Quantitation Assay, Multiplex Assay, Fluorescence, Sequencing, Immunocytochemistry

    Effects of integration inhibitor L-731,988. (A) Complete inhibition of virus production by integrase inhibitor L-731,988. Activated CD4 + T cells were infected with the X4-Env-pseudotyped HIV-1 reporter virus in the absence (open circles) or the

    Journal:

    Article Title: Kinetics of Human Immunodeficiency Virus Type 1 Decay following Entry into Resting CD4+ T Cells

    doi: 10.1128/JVI.79.4.2199-2210.2005

    Figure Lengend Snippet: Effects of integration inhibitor L-731,988. (A) Complete inhibition of virus production by integrase inhibitor L-731,988. Activated CD4 + T cells were infected with the X4-Env-pseudotyped HIV-1 reporter virus in the absence (open circles) or the

    Article Snippet: Reporter virus particles coated with HIV-1 envelope were generated by transfecting 30 × 106 293 T cells in a T150 flask with 20 μg of viral vector and 10 μg of an X4 HIV-1 envelope expression vector by using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions.

    Techniques: Inhibition, Infection

    Decay of HIV-1 in resting CD4 + T cells. (A) Schematic representation of experimental strategy for studying HIV-1 decay in resting CD4 + T cells. Resting CD4 + T cells were infected, and 4 h later the cells were treated with the fusion

    Journal:

    Article Title: Kinetics of Human Immunodeficiency Virus Type 1 Decay following Entry into Resting CD4+ T Cells

    doi: 10.1128/JVI.79.4.2199-2210.2005

    Figure Lengend Snippet: Decay of HIV-1 in resting CD4 + T cells. (A) Schematic representation of experimental strategy for studying HIV-1 decay in resting CD4 + T cells. Resting CD4 + T cells were infected, and 4 h later the cells were treated with the fusion

    Article Snippet: Reporter virus particles coated with HIV-1 envelope were generated by transfecting 30 × 106 293 T cells in a T150 flask with 20 μg of viral vector and 10 μg of an X4 HIV-1 envelope expression vector by using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions.

    Techniques: Infection

    In vitro model of acutely infected resting CD4 + T cells. (A) Recombinant HIV-1 vector used for infection of resting CD4 + T cells. Enhanced GFP was inserted in frame into the HIV-1 env gene in place of nucleotides 6348 to 7251 to produce

    Journal:

    Article Title: Kinetics of Human Immunodeficiency Virus Type 1 Decay following Entry into Resting CD4+ T Cells

    doi: 10.1128/JVI.79.4.2199-2210.2005

    Figure Lengend Snippet: In vitro model of acutely infected resting CD4 + T cells. (A) Recombinant HIV-1 vector used for infection of resting CD4 + T cells. Enhanced GFP was inserted in frame into the HIV-1 env gene in place of nucleotides 6348 to 7251 to produce

    Article Snippet: Reporter virus particles coated with HIV-1 envelope were generated by transfecting 30 × 106 293 T cells in a T150 flask with 20 μg of viral vector and 10 μg of an X4 HIV-1 envelope expression vector by using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions.

    Techniques: In Vitro, Infection, Recombinant, Plasmid Preparation

    Decay of HIV-1 before the completion of reverse transcription. (A) Experimental strategy for measuring the kinetics of HIV-1 decay in relation to the completion of reverse transcription. In these experiments, 3TC was added 4 h prior to activation and

    Journal:

    Article Title: Kinetics of Human Immunodeficiency Virus Type 1 Decay following Entry into Resting CD4+ T Cells

    doi: 10.1128/JVI.79.4.2199-2210.2005

    Figure Lengend Snippet: Decay of HIV-1 before the completion of reverse transcription. (A) Experimental strategy for measuring the kinetics of HIV-1 decay in relation to the completion of reverse transcription. In these experiments, 3TC was added 4 h prior to activation and

    Article Snippet: Reporter virus particles coated with HIV-1 envelope were generated by transfecting 30 × 106 293 T cells in a T150 flask with 20 μg of viral vector and 10 μg of an X4 HIV-1 envelope expression vector by using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions.

    Techniques: Activation Assay

    Decay of integration-competent HIV-1 in resting CD4 + T cells. (A) Experimental strategy for monitoring the functional decay of full-length, integration-competent viral DNA by blocking integration with L-731,988 upon T-cell activation. 3TC was

    Journal:

    Article Title: Kinetics of Human Immunodeficiency Virus Type 1 Decay following Entry into Resting CD4+ T Cells

    doi: 10.1128/JVI.79.4.2199-2210.2005

    Figure Lengend Snippet: Decay of integration-competent HIV-1 in resting CD4 + T cells. (A) Experimental strategy for monitoring the functional decay of full-length, integration-competent viral DNA by blocking integration with L-731,988 upon T-cell activation. 3TC was

    Article Snippet: Reporter virus particles coated with HIV-1 envelope were generated by transfecting 30 × 106 293 T cells in a T150 flask with 20 μg of viral vector and 10 μg of an X4 HIV-1 envelope expression vector by using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions.

    Techniques: Functional Assay, Blocking Assay, Activation Assay

    Viability of IPCs cultured with HIV-1 or HSV. Viable cells were counted by trypan blue staining. Percentages of viable IPCs cultured under different conditions were indicated. H.I., heat inactivated.

    Journal: Journal of Virology

    Article Title: Natural Alpha Interferon-Producing Cells Respond to Human Immunodeficiency Virus Type 1 with Alpha Interferon Production and Maturation into Dendritic Cells

    doi: 10.1128/JVI.77.6.3777-3784.2003

    Figure Lengend Snippet: Viability of IPCs cultured with HIV-1 or HSV. Viable cells were counted by trypan blue staining. Percentages of viable IPCs cultured under different conditions were indicated. H.I., heat inactivated.

    Article Snippet: IPCs were cultured at 2 × 105 /200 μl with HIV-1 adjusted to an equivalent of 100 ng of HIV-1 p24 or HSV, and the culture supernatants were collected after 48 h. IFN-α in the supernatants was measured by a human IFN-α ELISA kit (BioSource International, Camarillo, Calif.).

    Techniques: Cell Culture, Staining

    (A) IFN-α production by IPCs cultured with HIV-1 or HSV. IPCs were stimulated with HIV-1 or HSV for 48 h, and the concentrations of IFN-α in the supernatants were measured by ELISA. (B) Effect of anti-CD4 MAbs on HIV-1-induced IFN-α production by IPCs. (C) Effect of anti-IFN-α MAb on HIV-1 production.

    Journal: Journal of Virology

    Article Title: Natural Alpha Interferon-Producing Cells Respond to Human Immunodeficiency Virus Type 1 with Alpha Interferon Production and Maturation into Dendritic Cells

    doi: 10.1128/JVI.77.6.3777-3784.2003

    Figure Lengend Snippet: (A) IFN-α production by IPCs cultured with HIV-1 or HSV. IPCs were stimulated with HIV-1 or HSV for 48 h, and the concentrations of IFN-α in the supernatants were measured by ELISA. (B) Effect of anti-CD4 MAbs on HIV-1-induced IFN-α production by IPCs. (C) Effect of anti-IFN-α MAb on HIV-1 production.

    Article Snippet: IPCs were cultured at 2 × 105 /200 μl with HIV-1 adjusted to an equivalent of 100 ng of HIV-1 p24 or HSV, and the culture supernatants were collected after 48 h. IFN-α in the supernatants was measured by a human IFN-α ELISA kit (BioSource International, Camarillo, Calif.).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

    Expression of HIV-1 coreceptors and DC-SIGN on IPCs. (A) Detection of mRNA for CXCR4, CCR5, and DC-SIGN by RT-PCR in blood DC subsets. Cells were isolated by cell sorting to the purity of > 99%. N.C., negative control. (B) Expression of CXCR4 and CCR5 on purified IPCs. IPCs were stained with FITC-conjugated anti-CXCR4 or anti-CCR5 MAb. The dotted lines represent staining with isotype-matched control MAbs.

    Journal: Journal of Virology

    Article Title: Natural Alpha Interferon-Producing Cells Respond to Human Immunodeficiency Virus Type 1 with Alpha Interferon Production and Maturation into Dendritic Cells

    doi: 10.1128/JVI.77.6.3777-3784.2003

    Figure Lengend Snippet: Expression of HIV-1 coreceptors and DC-SIGN on IPCs. (A) Detection of mRNA for CXCR4, CCR5, and DC-SIGN by RT-PCR in blood DC subsets. Cells were isolated by cell sorting to the purity of > 99%. N.C., negative control. (B) Expression of CXCR4 and CCR5 on purified IPCs. IPCs were stained with FITC-conjugated anti-CXCR4 or anti-CCR5 MAb. The dotted lines represent staining with isotype-matched control MAbs.

    Article Snippet: IPCs were cultured at 2 × 105 /200 μl with HIV-1 adjusted to an equivalent of 100 ng of HIV-1 p24 or HSV, and the culture supernatants were collected after 48 h. IFN-α in the supernatants was measured by a human IFN-α ELISA kit (BioSource International, Camarillo, Calif.).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Isolation, FACS, Negative Control, Purification, Staining

    Productive infection of NL4-3 or JR-CSF in IPCs or CD11c + DCs. The levels of HIV-1 p24 antigen in supernatants were measured by ELISA.

    Journal: Journal of Virology

    Article Title: Natural Alpha Interferon-Producing Cells Respond to Human Immunodeficiency Virus Type 1 with Alpha Interferon Production and Maturation into Dendritic Cells

    doi: 10.1128/JVI.77.6.3777-3784.2003

    Figure Lengend Snippet: Productive infection of NL4-3 or JR-CSF in IPCs or CD11c + DCs. The levels of HIV-1 p24 antigen in supernatants were measured by ELISA.

    Article Snippet: IPCs were cultured at 2 × 105 /200 μl with HIV-1 adjusted to an equivalent of 100 ng of HIV-1 p24 or HSV, and the culture supernatants were collected after 48 h. IFN-α in the supernatants was measured by a human IFN-α ELISA kit (BioSource International, Camarillo, Calif.).

    Techniques: Infection, Enzyme-linked Immunosorbent Assay

    HIV-1 provirus integration assay. (A) Detection of the integrated form of viral DNA by using Alu -LTR PCR. Genomic DNAs from IPCs or phytohemagglutinin-stimulated PBMCs were subjected to in duplicate to PCR with nested 5′ primers from conserved human Alu and 3′ primers from conserved HIV-1 LTR sequences. The diluted PCR product was further subjected to the second PCR by using nested HIV-1 LTR specific primers. (B) Results of Alu -LTR PCR of genomic DNAs from pre-DC2s and phytohemagglutinin (PHA)-stimulated PBMCs. Viruses heat inactivated at 56°C for 1 h were used as a negative control.

    Journal: Journal of Virology

    Article Title: Natural Alpha Interferon-Producing Cells Respond to Human Immunodeficiency Virus Type 1 with Alpha Interferon Production and Maturation into Dendritic Cells

    doi: 10.1128/JVI.77.6.3777-3784.2003

    Figure Lengend Snippet: HIV-1 provirus integration assay. (A) Detection of the integrated form of viral DNA by using Alu -LTR PCR. Genomic DNAs from IPCs or phytohemagglutinin-stimulated PBMCs were subjected to in duplicate to PCR with nested 5′ primers from conserved human Alu and 3′ primers from conserved HIV-1 LTR sequences. The diluted PCR product was further subjected to the second PCR by using nested HIV-1 LTR specific primers. (B) Results of Alu -LTR PCR of genomic DNAs from pre-DC2s and phytohemagglutinin (PHA)-stimulated PBMCs. Viruses heat inactivated at 56°C for 1 h were used as a negative control.

    Article Snippet: IPCs were cultured at 2 × 105 /200 μl with HIV-1 adjusted to an equivalent of 100 ng of HIV-1 p24 or HSV, and the culture supernatants were collected after 48 h. IFN-α in the supernatants was measured by a human IFN-α ELISA kit (BioSource International, Camarillo, Calif.).

    Techniques: Polymerase Chain Reaction, Negative Control

    (A) The expression of CD80 and CD86 on IPCs. IPCs cultured with HIV-1, HSV, or medium alone for 48 h were stained with FITC-conjugated anti-CD80 or anti-CD86 MAbs and subjected to flow cytometric analysis. (B) Morphological changes of IPCs cultured with HIV-1 or HSV by using Giemsa staining. Freshly isolated IPCs showed plasmacytoid morphology, whereas IPCs cultured with HIV-1 and HSV for 2 days acquired DC morphology with prominent dendrites. H.I., heat inactivated.

    Journal: Journal of Virology

    Article Title: Natural Alpha Interferon-Producing Cells Respond to Human Immunodeficiency Virus Type 1 with Alpha Interferon Production and Maturation into Dendritic Cells

    doi: 10.1128/JVI.77.6.3777-3784.2003

    Figure Lengend Snippet: (A) The expression of CD80 and CD86 on IPCs. IPCs cultured with HIV-1, HSV, or medium alone for 48 h were stained with FITC-conjugated anti-CD80 or anti-CD86 MAbs and subjected to flow cytometric analysis. (B) Morphological changes of IPCs cultured with HIV-1 or HSV by using Giemsa staining. Freshly isolated IPCs showed plasmacytoid morphology, whereas IPCs cultured with HIV-1 and HSV for 2 days acquired DC morphology with prominent dendrites. H.I., heat inactivated.

    Article Snippet: IPCs were cultured at 2 × 105 /200 μl with HIV-1 adjusted to an equivalent of 100 ng of HIV-1 p24 or HSV, and the culture supernatants were collected after 48 h. IFN-α in the supernatants was measured by a human IFN-α ELISA kit (BioSource International, Camarillo, Calif.).

    Techniques: Expressing, Cell Culture, Staining, Flow Cytometry, Isolation

    (A) Red represents MT2 cells, blue represents TZM-bl cells, black represents H9 cells, and green represents 293T cells. The X-axis is the time of infection. The Y-axis represents log 10 of the HIV-1 pol load, and the units are indicated in the title of the Y-axis. Connecting curves are indicated. (B) Red represents MT2 cells, blue represents TZM-bl cells, black represents H9 cells, and green represents 293T cells. The X-axis is the time of infection. The Y-axis shows the fold increase in the transcription level in the HERV-K env region calculated by the 2 −ΔΔCT method. Standard errors for triplicate experiments are indicated. * P

    Journal: Frontiers in Microbiology

    Article Title: Infection by Diverse HIV-1 Subtypes Leads to Different Elevations in HERV-K Transcriptional Levels in Human T Cell Lines

    doi: 10.3389/fmicb.2021.662573

    Figure Lengend Snippet: (A) Red represents MT2 cells, blue represents TZM-bl cells, black represents H9 cells, and green represents 293T cells. The X-axis is the time of infection. The Y-axis represents log 10 of the HIV-1 pol load, and the units are indicated in the title of the Y-axis. Connecting curves are indicated. (B) Red represents MT2 cells, blue represents TZM-bl cells, black represents H9 cells, and green represents 293T cells. The X-axis is the time of infection. The Y-axis shows the fold increase in the transcription level in the HERV-K env region calculated by the 2 −ΔΔCT method. Standard errors for triplicate experiments are indicated. * P

    Article Snippet: Tat was detected using HIV-1 Tat monoclonal antibody (Thermo Fisher.

    Techniques: Infection

    (A) Blue represents HIV-1B subtype-infected persons, red represents HIV-1 CRF01_AE subtype-infected persons, purple represents HIV-1 CRF07_BC-infected persons, yellow represents other CRFs and URFS-infected persons, and green represents the healthy population control group. The Y-axis is ΔCT = CT HERV−K −CT β− actin . * P

    Journal: Frontiers in Microbiology

    Article Title: Infection by Diverse HIV-1 Subtypes Leads to Different Elevations in HERV-K Transcriptional Levels in Human T Cell Lines

    doi: 10.3389/fmicb.2021.662573

    Figure Lengend Snippet: (A) Blue represents HIV-1B subtype-infected persons, red represents HIV-1 CRF01_AE subtype-infected persons, purple represents HIV-1 CRF07_BC-infected persons, yellow represents other CRFs and URFS-infected persons, and green represents the healthy population control group. The Y-axis is ΔCT = CT HERV−K −CT β− actin . * P

    Article Snippet: Tat was detected using HIV-1 Tat monoclonal antibody (Thermo Fisher.

    Techniques: Infection

    (A) DAPI was used to stain and label the nuclei, green fluorescent proteins (GFP) with a special probe were used to stain and label HEVR-K env region mRNA, and red fluorescent proteins (RFPs) with a special probe were used to stain and label HIV-1 gag-pol region mRNA. The positive and negative controls used standard control probes provided in the kit (ACD). (B) MT2 cells in both the IIIB group and GX002 group exhibited notable cytopathic effects (CPEs) after 48 h of infection. The microscopic view of uninfected MT2 cells is shown.

    Journal: Frontiers in Microbiology

    Article Title: Infection by Diverse HIV-1 Subtypes Leads to Different Elevations in HERV-K Transcriptional Levels in Human T Cell Lines

    doi: 10.3389/fmicb.2021.662573

    Figure Lengend Snippet: (A) DAPI was used to stain and label the nuclei, green fluorescent proteins (GFP) with a special probe were used to stain and label HEVR-K env region mRNA, and red fluorescent proteins (RFPs) with a special probe were used to stain and label HIV-1 gag-pol region mRNA. The positive and negative controls used standard control probes provided in the kit (ACD). (B) MT2 cells in both the IIIB group and GX002 group exhibited notable cytopathic effects (CPEs) after 48 h of infection. The microscopic view of uninfected MT2 cells is shown.

    Article Snippet: Tat was detected using HIV-1 Tat monoclonal antibody (Thermo Fisher.

    Techniques: Staining, Infection

    (A) Red represents the GX002 group, blue represents the IIB group, green represents the NL4-3 group. The X-axis is the time of infection. The Y-axis represents log 10 of the HIV-1 pol load, and the units are indicated in the title of the Y-axis. Standard errors for triplicate experiments are indicated. (B) Red represents the GX002 group, blue represents the IIB group, green represents the NL4-3 group. The X-axis is the time of infection. The Y-axis represents the value of p24 antigen, and the units are indicated in the title of the Y-axis. Standard errors for triplicate experiments are indicated. (C) The bars represent the groups indicated in the figure. The X-axis is the time of infection. The Y-axis shows the fold increase in transcription level in the HERV-K gag, pol, env region calculated by the 2 −ΔΔCT method. Standard errors for triplicate experiments are indicated. * P

    Journal: Frontiers in Microbiology

    Article Title: Infection by Diverse HIV-1 Subtypes Leads to Different Elevations in HERV-K Transcriptional Levels in Human T Cell Lines

    doi: 10.3389/fmicb.2021.662573

    Figure Lengend Snippet: (A) Red represents the GX002 group, blue represents the IIB group, green represents the NL4-3 group. The X-axis is the time of infection. The Y-axis represents log 10 of the HIV-1 pol load, and the units are indicated in the title of the Y-axis. Standard errors for triplicate experiments are indicated. (B) Red represents the GX002 group, blue represents the IIB group, green represents the NL4-3 group. The X-axis is the time of infection. The Y-axis represents the value of p24 antigen, and the units are indicated in the title of the Y-axis. Standard errors for triplicate experiments are indicated. (C) The bars represent the groups indicated in the figure. The X-axis is the time of infection. The Y-axis shows the fold increase in transcription level in the HERV-K gag, pol, env region calculated by the 2 −ΔΔCT method. Standard errors for triplicate experiments are indicated. * P

    Article Snippet: Tat was detected using HIV-1 Tat monoclonal antibody (Thermo Fisher.

    Techniques: Infection