hiv type 1  (Sino Biological)


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    Name:
    Human Immunodeficiency Virus type 1 Gag p24 Antibody Mouse MAb
    Description:
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant HIV 1 Gag p24 HIV 1 Gag p24 Catalog 11695 V08E ACI05538 1 Pro 133 Leu 363 The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    Catalog Number:
    11695-MM13
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    HIV
    Applications:
    WB,ELISA
    Immunogen:
    Recombinant HIV-1 Gag-P24 protein (Catalog#11695-V08E)
    Product Aliases:
    Anti-Gag-p24 Antibody
    Antibody Type:
    MAb
    Host:
    Mouse
    Isotype:
    Mouse IgG1
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    Structured Review

    Sino Biological hiv type 1
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant HIV 1 Gag p24 HIV 1 Gag p24 Catalog 11695 V08E ACI05538 1 Pro 133 Leu 363 The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    https://www.bioz.com/result/hiv type 1/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv type 1 - by Bioz Stars, 2021-06
    93/100 stars

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    other:

    Article Title: The Rhinolophus affinis bat ACE2 and multiple animal orthologs are functional receptors for bat coronavirus RaTG13 and SARS-CoV-2
    Article Snippet: AntibodiesRabbit polyclonal against SARS S1 antibodies (#40150-T62), mouse monoclonal against SARS S1 antibody (#40150-MM02), rabbit polyclonal against SARS-CoV-2 RBD antibodies(#40592-T62), rabbit polyclonal against SARS-CoV-2 S2 antibodies(#40590-T62), rabbit polyclonal against HIV-1 Gag-p24 antibody (11695-RB01) were purchased from Sino Biological Inc. (Beijing, China).

    Isolation:

    Article Title: Deep Coverage Tissue and Cellular Proteomics Revealed IL-1β Can Independently Induce the Secretion of TNF-Associated Proteins from Human Synoviocytes.
    Article Snippet: Cell culture and treatmentPrimaryHSwere purchased from ScienCell (Carlsbad, CA; lot number 2070), and maintained in Synoviocyte Medium (ScienCell) consisting of 500 ml of basal medium, 10 ml of FBS, 5 ml of synoviocyte growth supplement, and 5 ml of penicillin/streptomycin solution (all from ScienCell), as we previously described (2). .. Per the manufacturer’s instructions, these primary HS were isolated from a healthy nonarthritic subject; they were CD90 and fibronectin positive, although negative for HIV type 1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. ..

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  • 96
    Sino Biological hiv p24
    Characterization of SARS-CoV-2 Spike pseudovirus. A. Incorporation of SARS-CoV-2 S protein into pseudoviruses. VSV-G and S pseudoviruses were pelleted as described in Materials and Methods and subjected to SDS-PAGE, immunoblotted with anti-Spike and <t>HIV</t> <t>p24</t> as control. B. S pseudovirus transduction of 293T, 293T-ACE2, Vero E6 and Huh 7 cells. 293T, 293T-ACE2, Vero E6 and Huh 7 cells were mock infected or infected with S pseudovirus, VSV-G pseudovirus expressing NanoLuc luciferase. At 48 h post transduction, luciferase activity was measured. Values are mean ± SD and are representative of three independent experiments. C. 293T, 293T-ACE2, Vero E6 and Huh 7 cells transduced with S pseudovirus expressing GFP. Bright field was included to show the presence of cells. Bar, 20μm.
    Hiv P24, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv p24/product/Sino Biological
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv p24 - by Bioz Stars, 2021-06
    96/100 stars
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    93
    Sino Biological hiv 1 gag p24
    STAT1 or STAT3 inhibitors suppress <t>HIV-1</t> infection of MDM. MDM were pretreated with 100 nM Fludarabine (STAT1 inhibitor) or 50 nM LLL12 (STAT3 inhibitor) 24 h before infection and on PID 1, 4, and 7. ELISA was used to measure supernatant <t>p24</t> on PID 4, 7, and 10 in cultures treated with (A) Fludarabine and (B) LLL12. Bars represent mean ± SEM; * p
    Hiv 1 Gag P24, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv 1 gag p24/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv 1 gag p24 - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    N/A
    Produced in rabbits immunized with purified recombinant HIV HIV 1 p24 Protein group M subtype B strain 92418 Catalog 11695 V08E ACI05538 1 Pro133 Leu363 and purified by antigen affinity
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    Characterization of SARS-CoV-2 Spike pseudovirus. A. Incorporation of SARS-CoV-2 S protein into pseudoviruses. VSV-G and S pseudoviruses were pelleted as described in Materials and Methods and subjected to SDS-PAGE, immunoblotted with anti-Spike and HIV p24 as control. B. S pseudovirus transduction of 293T, 293T-ACE2, Vero E6 and Huh 7 cells. 293T, 293T-ACE2, Vero E6 and Huh 7 cells were mock infected or infected with S pseudovirus, VSV-G pseudovirus expressing NanoLuc luciferase. At 48 h post transduction, luciferase activity was measured. Values are mean ± SD and are representative of three independent experiments. C. 293T, 293T-ACE2, Vero E6 and Huh 7 cells transduced with S pseudovirus expressing GFP. Bright field was included to show the presence of cells. Bar, 20μm.

    Journal: bioRxiv

    Article Title: Cytoplasmic domain and enzymatic activity of ACE2 is not required for PI4KB dependent endocytosis entry of SARS-CoV-2 into host cells

    doi: 10.1101/2021.03.01.433503

    Figure Lengend Snippet: Characterization of SARS-CoV-2 Spike pseudovirus. A. Incorporation of SARS-CoV-2 S protein into pseudoviruses. VSV-G and S pseudoviruses were pelleted as described in Materials and Methods and subjected to SDS-PAGE, immunoblotted with anti-Spike and HIV p24 as control. B. S pseudovirus transduction of 293T, 293T-ACE2, Vero E6 and Huh 7 cells. 293T, 293T-ACE2, Vero E6 and Huh 7 cells were mock infected or infected with S pseudovirus, VSV-G pseudovirus expressing NanoLuc luciferase. At 48 h post transduction, luciferase activity was measured. Values are mean ± SD and are representative of three independent experiments. C. 293T, 293T-ACE2, Vero E6 and Huh 7 cells transduced with S pseudovirus expressing GFP. Bright field was included to show the presence of cells. Bar, 20μm.

    Article Snippet: Antibodies and reagents Antibodies used in this study include: SARS-CoV-2 spike (GeneTex, Taiwan), HA tag, EEA1 (Cell Signaling Technology, Danvers, MA), HIV-p24 (SinoBiological, Beijing, China), β-actin (ABclonal, Woburn, MA), CHC (Santa Cruz Biotechnology, Dallas, TX), CAV1(Proteintech, Chicago, IL) and ACE2 (Abways Technology, Shanghai, China).

    Techniques: SDS Page, Transduction, Infection, Expressing, Luciferase, Activity Assay

    Spike incorporation into Spike-pseudotyped lentiviral particles. Spike D614 and Spike G614 protein incorporation rate into pseudotyped lentiviral particles was quantified by densitometry of total Spike expression (cleaved and uncleaved) normalized to expression of the lentiviral capsid protein p24. Significance testing was done with an unpaired two-tailed t- test.

    Journal: eLife

    Article Title: The Spike D614G mutation increases SARS-CoV-2 infection of multiple human cell types

    doi: 10.7554/eLife.65365

    Figure Lengend Snippet: Spike incorporation into Spike-pseudotyped lentiviral particles. Spike D614 and Spike G614 protein incorporation rate into pseudotyped lentiviral particles was quantified by densitometry of total Spike expression (cleaved and uncleaved) normalized to expression of the lentiviral capsid protein p24. Significance testing was done with an unpaired two-tailed t- test.

    Article Snippet: Primary antibody incubations were performed overnight at 4°C using the following antibodies: rabbit anti-GAPDH 14C10 (RRID: AB_561053 ) (0.1 μg/mL, Cell Signaling 2118S), rabbit p24 monoclonal antibody clone 002, which recognizes lentiviral capsid protein (1 μg/mL, Sino Biological 11695-V08E), and mouse anti-rhodopsin antibody clone 1D4 (RRID: AB_10010560) (1 μg/mL, Novus NBP1-47602), which recognizes the C9-tag added to the Spike proteins.

    Techniques: Expressing, Two Tailed Test

    STAT1 or STAT3 inhibitors suppress HIV-1 infection of MDM. MDM were pretreated with 100 nM Fludarabine (STAT1 inhibitor) or 50 nM LLL12 (STAT3 inhibitor) 24 h before infection and on PID 1, 4, and 7. ELISA was used to measure supernatant p24 on PID 4, 7, and 10 in cultures treated with (A) Fludarabine and (B) LLL12. Bars represent mean ± SEM; * p

    Journal: AIDS Research and Human Retroviruses

    Article Title: HIV-1 Infection Primes Macrophages Through STAT Signaling to Promote Enhanced Inflammation and Viral Replication

    doi: 10.1089/aid.2016.0273

    Figure Lengend Snippet: STAT1 or STAT3 inhibitors suppress HIV-1 infection of MDM. MDM were pretreated with 100 nM Fludarabine (STAT1 inhibitor) or 50 nM LLL12 (STAT3 inhibitor) 24 h before infection and on PID 1, 4, and 7. ELISA was used to measure supernatant p24 on PID 4, 7, and 10 in cultures treated with (A) Fludarabine and (B) LLL12. Bars represent mean ± SEM; * p

    Article Snippet: HIV-1 Gag p24 in culture supernatants was measured by ELISA (Sino Biological, Daxing, China).

    Techniques: Infection, Enzyme-linked Immunosorbent Assay

    STAT1 and STAT3 are involved in HIV-1-induced priming. MDM were treated with 100 nM Fludarabine (STAT1 inhibitor) or 50 nM LLL12 (STAT3 inhibitor) 24 h before infection and on PID 1, 4, and 7. Ten days postinfection, cells were activated with 1 μg/mL CL097 for 6 h. Intracellular p24 and TNF were measured by flow cytometry. Bars represent mean ± SEM MFI of TNF in uninfected cells and infected cells gated on either p24-negative or p24-positive populations; * p

    Journal: AIDS Research and Human Retroviruses

    Article Title: HIV-1 Infection Primes Macrophages Through STAT Signaling to Promote Enhanced Inflammation and Viral Replication

    doi: 10.1089/aid.2016.0273

    Figure Lengend Snippet: STAT1 and STAT3 are involved in HIV-1-induced priming. MDM were treated with 100 nM Fludarabine (STAT1 inhibitor) or 50 nM LLL12 (STAT3 inhibitor) 24 h before infection and on PID 1, 4, and 7. Ten days postinfection, cells were activated with 1 μg/mL CL097 for 6 h. Intracellular p24 and TNF were measured by flow cytometry. Bars represent mean ± SEM MFI of TNF in uninfected cells and infected cells gated on either p24-negative or p24-positive populations; * p

    Article Snippet: HIV-1 Gag p24 in culture supernatants was measured by ELISA (Sino Biological, Daxing, China).

    Techniques: Infection, Flow Cytometry, Cytometry

    Only productively infected (p24 + ) MDM are primed. (A) MDM were infected with 5000 TCID 50 HIV-1 NL4-3-BaL-HSA encoding the murine heat-stable antigen (HSA; CD24), which is expressed on the surface of productively infected cells. CD24 + and CD24 − cells were sorted using magnetic beads and expression of HIV-1 gag p24 was measured by western blot to confirm efficient sorting of infected cells. (B) Uninfected and infected (CD24 − and CD24 + ) MDM were replated and stimulated with 10 ng/mL LPS for 6 h. ELISA measured secreted TNF. The experiment was conducted in one donor. ND, not detectable.

    Journal: AIDS Research and Human Retroviruses

    Article Title: HIV-1 Infection Primes Macrophages Through STAT Signaling to Promote Enhanced Inflammation and Viral Replication

    doi: 10.1089/aid.2016.0273

    Figure Lengend Snippet: Only productively infected (p24 + ) MDM are primed. (A) MDM were infected with 5000 TCID 50 HIV-1 NL4-3-BaL-HSA encoding the murine heat-stable antigen (HSA; CD24), which is expressed on the surface of productively infected cells. CD24 + and CD24 − cells were sorted using magnetic beads and expression of HIV-1 gag p24 was measured by western blot to confirm efficient sorting of infected cells. (B) Uninfected and infected (CD24 − and CD24 + ) MDM were replated and stimulated with 10 ng/mL LPS for 6 h. ELISA measured secreted TNF. The experiment was conducted in one donor. ND, not detectable.

    Article Snippet: HIV-1 Gag p24 in culture supernatants was measured by ELISA (Sino Biological, Daxing, China).

    Techniques: Infection, Magnetic Beads, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay