histrap ff crude column (GE Healthcare)
Name:
HisTrap FF
Description:
Catalog Number:
17525501
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None
Category:
HisTrap FF
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https://www.bioz.com/result/histrap ff crude column/product/GE Healthcare
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1) Product Images from "Bacterial expression of human kynurenine 3-monooxygenase: Solubility, activity, purification"
Article Title: Bacterial expression of human kynurenine 3-monooxygenase: Solubility, activity, purification
Journal: Protein Expression and Purification
doi: 10.1016/j.pep.2013.11.015

Figure Legend Snippet: Purified trKMO. (A) Chromatogram produced during NiNTA affinity purification of truncate KMO. (B) SDS-PAGE protein gel stained with simplyblue showing HisTrap purification of truncated human KMO protein, (a) Cell-free extract prior to affinity chromatography, (b) flowthrough proteins which did not bind to the column, (c) first column wash, (d) second column wash, (e) third column wash, (f) pure truncated human KMO protein (44 kDa) from the fractions under the chromatogram. (C) Cartoon illustrating the trKMO construct. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Techniques Used: Purification, Produced, Affinity Purification, SDS Page, Staining, Affinity Chromatography, Construct
2) Product Images from "Protein expression, characterization, crystallization and preliminary X-ray crystallographic analysis of a Fic protein from Clostridium difficile"
Article Title: Protein expression, characterization, crystallization and preliminary X-ray crystallographic analysis of a Fic protein from Clostridium difficile
Journal: Acta Crystallographica. Section F, Structural Biology Communications
doi: 10.1107/S2053230X1400987X

Figure Legend Snippet: Purification and oligomeric state of CdFic SE/AA . ( a ) Coomassie-stained 4–15% gradient SDS–PAGE of purified CdFic SE/AA . Lane M , molecular-weight marker (labelled in kDa). Lane 1, elution from the HisTrap affinity column, 1.8 µg
Techniques Used: Purification, Staining, SDS Page, Molecular Weight, Marker, Affinity Column
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