histone h 4  (Millipore)


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    Name:
    Histone
    Description:
    Mixture of histones H1 H2A H2B H3 and H4 isolated from calf thymus Histones are a group of DNA binding proteins that are characterized by relatively high levels of lysine and arginine Five different fractions of histones have been isolated and characterized These are named as H1 H2A H2B H3 and H4 The H1 fraction is lysine rich while the H2A and H2B are slightly lysine rich The H3 and H4 fractions are arginine rich The molecular weights of histones are approximately 11 to 21 kDa depending on the fraction
    Catalog Number:
    10223565001
    Price:
    None
    Applications:
    Histone from calf thymus has been used:. in in vitro kinase assay to check the phosphorylation of histones by protein kinase A.. to check the in vitro methyltransferase activity of protein argininemethyltransferases from Oryza sativa (OsPRMTs).. in anti-histone ELISA.
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    Structured Review

    Millipore histone h 4
    Mixture of histones H1 H2A H2B H3 and H4 isolated from calf thymus Histones are a group of DNA binding proteins that are characterized by relatively high levels of lysine and arginine Five different fractions of histones have been isolated and characterized These are named as H1 H2A H2B H3 and H4 The H1 fraction is lysine rich while the H2A and H2B are slightly lysine rich The H3 and H4 fractions are arginine rich The molecular weights of histones are approximately 11 to 21 kDa depending on the fraction
    https://www.bioz.com/result/histone h 4/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    histone h 4 - by Bioz Stars, 2020-09
    99/100 stars

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    immunoprecipitation
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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: A time-series analysis of altered histone H3 acetylation and gene expression during the course of MMAIII-induced malignant transformation of urinary bladder cells
    Article Snippet: .. Here, we verified the result by ELISA assay and showed that the total acetylated histone H4 was decreased based on the acetylation levels on histone H4 lysine K5, K8, K12 and K16 at the N-terminal of tails over the time course of MMAIII treatment ( ). ..

    Ex Vivo:

    Article Title: MST1 Promotes Apoptosis through Phosphorylation of Histone H2AX
    Article Snippet: .. Taken together, these results suggested that both full-length MST1 and the MST1 kinase domain can interact with histone H2AX, and either the overexpressed MST1-FL or MST1-NT can induce H2AX phosphorylation ex vivo , which is depen-dent on MST1 kinase activity. .. To study the dependence of MST1 cleavage in the induction of H2AX phosphorylation, pCE-Myc-MST1-FL and pCE-Myc-MST1-NT plasmids were each transfected into HeLa cells, and cells were treated or not with the caspase-3 inhibitor, Z-DEVD-fmk.

    Incubation:

    Article Title: Cortical recruitment of nonmuscle myosin II in early syncytial Drosophila embryos
    Article Snippet: .. The embryos were incubated with antihistone used at 1:500 (Chemicon). .. Bound antibody were detected using a goat anti–rabbit IgG secondary antibody conjugated to AlexaTM 488 (Molecular Probes).

    Activity Assay:

    Article Title: MST1 Promotes Apoptosis through Phosphorylation of Histone H2AX
    Article Snippet: .. Taken together, these results suggested that both full-length MST1 and the MST1 kinase domain can interact with histone H2AX, and either the overexpressed MST1-FL or MST1-NT can induce H2AX phosphorylation ex vivo , which is depen-dent on MST1 kinase activity. .. To study the dependence of MST1 cleavage in the induction of H2AX phosphorylation, pCE-Myc-MST1-FL and pCE-Myc-MST1-NT plasmids were each transfected into HeLa cells, and cells were treated or not with the caspase-3 inhibitor, Z-DEVD-fmk.

    Expressing:

    Article Title: Does p49/STRAP, a SRF-binding protein (SRFBP1), Modulate Cardiac Mitochondrial Function in Aging?
    Article Snippet: .. Acetylation of histone H4 on lysine 16 (H4K16) is a prevalent and reversible epigenetic, posttranslational chromatin modification in eukaryotes, which affects various gene expression levels ( ). .. The deacetylation of H4K16 that was induced by p49/STRAP overexpression might have also repressed the PGC-1a gene expression, which is an upstream regulator of the mitofusin genes, and further inhibited the expression of mitofusin genes.

    Modification:

    Article Title: Does p49/STRAP, a SRF-binding protein (SRFBP1), Modulate Cardiac Mitochondrial Function in Aging?
    Article Snippet: .. Acetylation of histone H4 on lysine 16 (H4K16) is a prevalent and reversible epigenetic, posttranslational chromatin modification in eukaryotes, which affects various gene expression levels ( ). .. The deacetylation of H4K16 that was induced by p49/STRAP overexpression might have also repressed the PGC-1a gene expression, which is an upstream regulator of the mitofusin genes, and further inhibited the expression of mitofusin genes.

    Binding Assay:

    Article Title: Statins inhibit tumor progression via an enhancer of zeste homolog 2-mediated epigenetic alteration in colorectal cancer
    Article Snippet: .. The antibodies used were: HDAC3 (Cell Signaling, MA, USA), HDAC5 (Santa-Cruz, Texas, USA), HDAC7 (Santa Cruz), EZH2 (BD Biosciences, CA, USA), sterol regulatory element binding protein 2 (SREBP2; Cayman, Michigan, USA), p27 (BD Biosciences), β-actin (Cell Signaling), Histone1 (Sigma). .. EZH2 suppression by small interfering RNA (siRNA) duplex oligo ribonucleotide EZH2 suppression was performed using siRNA and Stealth RNAi siRNA duplex oligo nucleotides (Invitrogen).

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  • 99
    Millipore histone h3
    DNA methylation affects ARL4C expression A. DNMT1, DNMT3a, DNMT3b ,  TET1, TET2, TET3,  and  GAPDH  mRNA levels in HeLaS3, NCI-H520, and SAS cells were measured by digital PCR and then copy numbers of these genes are shown.  B.  TET family knockout NCI-H520 cells were generated. Analysis of 5hmC levels in genomic DNA isolated from TET family knockout NCI-H520 cell clones was performed by dot blot assay using an anti-5hmC antibody. Anti-double strand (ds) DNA antibody was probed as a control.  C.  DNA methylation status of the  ARL4C  3’-UTR in TET family knockout NCI-H520 cells was examined by bisulfite pyrosequencing.  D. ARL4C  mRNA levels in TET family knockout NCI-H520 cells were measured by quantitative RT-PCR and relative levels of  ARL4C  mRNA expression were normalized to  GAPDH  and expressed as fold-changes compared with control cells. Cell lysates were probed with anti-ARL4C and anti-HSP90 antibodies.  E.  Control or TET family knockout NCI-H520 cells were placed in Transwell chamber for the migration assay. Migration activities were expressed as the percentage of control cells.  F.  Cell lysates of control or TET family knockout NCI-H520 cells expressing mock or ARL4C were probed with anti-ARL4C and anti-β-tubulin antibodies.  G.  Control or TET family knockout NCI-H520 cells expressing mock or ARL4C were placed in Transwell chamber for the migration assays. Migration activities are expressed as the percentage of control cells.  H.  Chromatin from control or TET family knockout NCI-H520 cells was immunoprecipitated with anti-control IgG or anti-CREB or anti-histone H3 (tri methyl K4) antibodies and the precipitates were analyzed by PCR for  Arl4c  3′-UTR (CpG No. 6 to No. 8.). Results are shown as means ± s.d. of three independent experiments.  * ,  P
    Histone H3, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/histone h3/product/Millipore
    Average 99 stars, based on 233 article reviews
    Price from $9.99 to $1999.99
    histone h3 - by Bioz Stars, 2020-09
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    93
    Millipore hati
    HDAC inhibition diminishes the effects of <t>SUV4-20</t> inhibition in typically prone SUM159 cells, while <t>HATi</t> facilitates SUV4-20 inhibition in typically resistant MCF7 cells. a Discrete H4 acetylations are increased rapidly upon HDAC inhibition. b Relative abundance of H4{K20me2} is not decreased in HDACi pretreated SUM159 cells. c Volcano plot of changes in proteoform abundance in SUM159 cells upon HDAC inhibition. Data points in the gray dashed squares indicate infinity fold change. d HDACi diminishes the effects of SUV4-20 inhibition on discrete H4{K20me2}. e The effect of HDACi then SUV4-20 inhibition on the abundance of
    Hati, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hati/product/Millipore
    Average 93 stars, based on 4 article reviews
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    90
    Millipore phosphorylated histone protein h2a variant x
    HDAC inhibition diminishes the effects of <t>SUV4-20</t> inhibition in typically prone SUM159 cells, while <t>HATi</t> facilitates SUV4-20 inhibition in typically resistant MCF7 cells. a Discrete H4 acetylations are increased rapidly upon HDAC inhibition. b Relative abundance of H4{K20me2} is not decreased in HDACi pretreated SUM159 cells. c Volcano plot of changes in proteoform abundance in SUM159 cells upon HDAC inhibition. Data points in the gray dashed squares indicate infinity fold change. d HDACi diminishes the effects of SUV4-20 inhibition on discrete H4{K20me2}. e The effect of HDACi then SUV4-20 inhibition on the abundance of
    Phosphorylated Histone Protein H2a Variant X, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated histone protein h2a variant x/product/Millipore
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    DNA methylation affects ARL4C expression A. DNMT1, DNMT3a, DNMT3b ,  TET1, TET2, TET3,  and  GAPDH  mRNA levels in HeLaS3, NCI-H520, and SAS cells were measured by digital PCR and then copy numbers of these genes are shown.  B.  TET family knockout NCI-H520 cells were generated. Analysis of 5hmC levels in genomic DNA isolated from TET family knockout NCI-H520 cell clones was performed by dot blot assay using an anti-5hmC antibody. Anti-double strand (ds) DNA antibody was probed as a control.  C.  DNA methylation status of the  ARL4C  3’-UTR in TET family knockout NCI-H520 cells was examined by bisulfite pyrosequencing.  D. ARL4C  mRNA levels in TET family knockout NCI-H520 cells were measured by quantitative RT-PCR and relative levels of  ARL4C  mRNA expression were normalized to  GAPDH  and expressed as fold-changes compared with control cells. Cell lysates were probed with anti-ARL4C and anti-HSP90 antibodies.  E.  Control or TET family knockout NCI-H520 cells were placed in Transwell chamber for the migration assay. Migration activities were expressed as the percentage of control cells.  F.  Cell lysates of control or TET family knockout NCI-H520 cells expressing mock or ARL4C were probed with anti-ARL4C and anti-β-tubulin antibodies.  G.  Control or TET family knockout NCI-H520 cells expressing mock or ARL4C were placed in Transwell chamber for the migration assays. Migration activities are expressed as the percentage of control cells.  H.  Chromatin from control or TET family knockout NCI-H520 cells was immunoprecipitated with anti-control IgG or anti-CREB or anti-histone H3 (tri methyl K4) antibodies and the precipitates were analyzed by PCR for  Arl4c  3′-UTR (CpG No. 6 to No. 8.). Results are shown as means ± s.d. of three independent experiments.  * ,  P

    Journal: Oncotarget

    Article Title: Epigenetic upregulation of ARL4C, due to DNA hypomethylation in the 3'-untranslated region, promotes tumorigenesis of lung squamous cell carcinoma

    doi: 10.18632/oncotarget.13147

    Figure Lengend Snippet: DNA methylation affects ARL4C expression A. DNMT1, DNMT3a, DNMT3b , TET1, TET2, TET3, and GAPDH mRNA levels in HeLaS3, NCI-H520, and SAS cells were measured by digital PCR and then copy numbers of these genes are shown. B. TET family knockout NCI-H520 cells were generated. Analysis of 5hmC levels in genomic DNA isolated from TET family knockout NCI-H520 cell clones was performed by dot blot assay using an anti-5hmC antibody. Anti-double strand (ds) DNA antibody was probed as a control. C. DNA methylation status of the ARL4C 3’-UTR in TET family knockout NCI-H520 cells was examined by bisulfite pyrosequencing. D. ARL4C mRNA levels in TET family knockout NCI-H520 cells were measured by quantitative RT-PCR and relative levels of ARL4C mRNA expression were normalized to GAPDH and expressed as fold-changes compared with control cells. Cell lysates were probed with anti-ARL4C and anti-HSP90 antibodies. E. Control or TET family knockout NCI-H520 cells were placed in Transwell chamber for the migration assay. Migration activities were expressed as the percentage of control cells. F. Cell lysates of control or TET family knockout NCI-H520 cells expressing mock or ARL4C were probed with anti-ARL4C and anti-β-tubulin antibodies. G. Control or TET family knockout NCI-H520 cells expressing mock or ARL4C were placed in Transwell chamber for the migration assays. Migration activities are expressed as the percentage of control cells. H. Chromatin from control or TET family knockout NCI-H520 cells was immunoprecipitated with anti-control IgG or anti-CREB or anti-histone H3 (tri methyl K4) antibodies and the precipitates were analyzed by PCR for Arl4c 3′-UTR (CpG No. 6 to No. 8.). Results are shown as means ± s.d. of three independent experiments. * , P

    Article Snippet: Sheared chromatin samples were diluted in ChIP dilution buffer (16.7 mM Tris/HCl [pH 8.0], 167 mM NaCl, 1.2 mM EDTA, and 1.1% Triton X-100) supplemented with protease inhibitors, and precleared with salmon sperm DNA/protein A-agarose (Millipore, Billerica, MA, USA) and incubated for 12 h at 4°C with 5 mg of histone H3 (tri methyl K4) (Millipore) and anti-CREB (Abcam) antibodies, or negative control IgG (Diagenode, Liége, Belgium).

    Techniques: DNA Methylation Assay, Expressing, Digital PCR, Knock-Out, Generated, Isolation, Clone Assay, Dot Blot, Quantitative RT-PCR, Migration, Immunoprecipitation, Polymerase Chain Reaction

    HDAC inhibition diminishes the effects of SUV4-20 inhibition in typically prone SUM159 cells, while HATi facilitates SUV4-20 inhibition in typically resistant MCF7 cells. a Discrete H4 acetylations are increased rapidly upon HDAC inhibition. b Relative abundance of H4{K20me2} is not decreased in HDACi pretreated SUM159 cells. c Volcano plot of changes in proteoform abundance in SUM159 cells upon HDAC inhibition. Data points in the gray dashed squares indicate infinity fold change. d HDACi diminishes the effects of SUV4-20 inhibition on discrete H4{K20me2}. e The effect of HDACi then SUV4-20 inhibition on the abundance of

    Journal: Epigenetics & Chromatin

    Article Title: The histone H4 proteoform dynamics in response to SUV4-20 inhibition reveals single molecule mechanisms of inhibitor resistance

    doi: 10.1186/s13072-018-0198-9

    Figure Lengend Snippet: HDAC inhibition diminishes the effects of SUV4-20 inhibition in typically prone SUM159 cells, while HATi facilitates SUV4-20 inhibition in typically resistant MCF7 cells. a Discrete H4 acetylations are increased rapidly upon HDAC inhibition. b Relative abundance of H4{K20me2} is not decreased in HDACi pretreated SUM159 cells. c Volcano plot of changes in proteoform abundance in SUM159 cells upon HDAC inhibition. Data points in the gray dashed squares indicate infinity fold change. d HDACi diminishes the effects of SUV4-20 inhibition on discrete H4{K20me2}. e The effect of HDACi then SUV4-20 inhibition on the abundance of

    Article Snippet: Combination of HATi with SUV4-20 inhibition MCF7 cells were treated with 100 µM HATi (CTK7A, Calbiochem) for 4 h, followed by addition of sufficient stock to give 100 µM HATi + 1 µM A-196 for another 2 h.

    Techniques: Inhibition