hispeed plasmid maxi prep kit  (Qiagen)


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    Name:
    HiSpeed Plasmid Maxi Kit
    Description:
    For ultrafast purification of up to 750 µg transfection grade plasmid or cosmid DNA Kit contents Qiagen HiSpeed Plasmid Maxi Kit 10 preps 150L to 250mL Culture Volume Transfection grade Plasmid or Cosmid DNA Purification Anion exchange Technology Manual Processing 60 min Time Run 750g Yield For Ultrafast Purification of up to 750g Transfection grade Plasmid or Cosmid DNA Includes 10 HiSpeed Maxi Tips 10 QIAfilter Maxi Cartridges 10 QIAprecipitator Maxi Modules plus Syringes Reagents Buffers Benefits Less than 60 minutes prep time Lysate clearing and isopropanol precipitation without centrifugation No risk of DNA pellet loss during precipitation Up to 750 µg yield of high copy plasmid DNA LyseBlue for optimum lysis and maximum DNA yield Large scale plasmid preparation without vacuum manifo
    Catalog Number:
    12662
    Price:
    300
    Category:
    HiSpeed Plasmid Kits
    Buy from Supplier


    Structured Review

    Qiagen hispeed plasmid maxi prep kit
    HiSpeed Plasmid Maxi Kit
    For ultrafast purification of up to 750 µg transfection grade plasmid or cosmid DNA Kit contents Qiagen HiSpeed Plasmid Maxi Kit 10 preps 150L to 250mL Culture Volume Transfection grade Plasmid or Cosmid DNA Purification Anion exchange Technology Manual Processing 60 min Time Run 750g Yield For Ultrafast Purification of up to 750g Transfection grade Plasmid or Cosmid DNA Includes 10 HiSpeed Maxi Tips 10 QIAfilter Maxi Cartridges 10 QIAprecipitator Maxi Modules plus Syringes Reagents Buffers Benefits Less than 60 minutes prep time Lysate clearing and isopropanol precipitation without centrifugation No risk of DNA pellet loss during precipitation Up to 750 µg yield of high copy plasmid DNA LyseBlue for optimum lysis and maximum DNA yield Large scale plasmid preparation without vacuum manifo
    https://www.bioz.com/result/hispeed plasmid maxi prep kit/product/Qiagen
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    hispeed plasmid maxi prep kit - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Article Snippet: This cloning system permits induction to 10 or more copies of these BACs per cell. .. An E. coli strain carrying each of these 25 pieces was inoculated into 150 ml of LB plus 12.5 μg/ml of chloramphenicol and 1X induction solution (Epicentre) and incubated at 37 °C for 16 h. The cultures were harvested, and the BACs were purified using a Qiagen HiSpeed Plasmid Maxi Kit.

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
    Article Snippet: The ABD of α-actinin-2 (CAA32078.1) was cloned into the DsRed2-c1 vector similarly, with the primers. .. Amplified cDNA was isolated and purified using a HiSpeed Plasmid Maxi Kit (Qiagen; Valencia, CA) following the manufacturer's instructions and prepared for the biolistic transfection of organotypic hippocampal slice cultures.

    Article Title: Characterisation of an engineered trastuzumab IgE antibody and effector cell mechanisms targeting HER2/neu-positive tumour cells
    Article Snippet: This cDNA was then cloned into two vectors based on a pTT vector backbone, one containing the epsilon heavy chain of IgE (humighae2, accession no: ; Kenten et al. 1982), the other containing the human kappa light chain constant region cDNA (IGKC, accession no: ) [ , ] ( ). .. For transfection into compatible HEK293 cells, vector DNA was produced using the HiSpeed Plasmid Maxi Kit® (Qiagen® ), according to the manufacturer's instructions.

    Article Title: Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
    Article Snippet: tRNA purification The gene encoding M. maripaludis tRNASec with the preceding sequence of the T7 promoter was cloned into the pUC18 plasmid, and the gene encoding M. maripaludis tRNASer UGA with the preceding sequence of the T7 promoter was cloned into the pUC19 plasmid. .. Plasmid DNA was purified using the HISpeed Plasmid Maxi kit (Qiagen).

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
    Article Snippet: Luciferase reporter constructs were generated by cloning LILRB1 promoter sequences into Nhe I and Xho I sites of pGL4.10 (Promega). .. All plasmids were confirmed by sequencing (Agencourt) and doubly purified from bacterial culture by HiSpeed Plasmid Maxi Kit (QIAGEN) followed by QIAquick PCR Purification Kit (QIAGEN) before transfection.

    Article Title: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB
    Article Snippet: Initial PCR products were cloned into pCR®-BluntII-TOPO® vectors using Zero Blunt TOPO PCR Cloning Kit (Invitrogen), sequenced and re-cloned into NanoBiT™ expression vectors with appropriate restriction enzyme sites, followed by ligation with T4 DNA ligase (Promega) overnight at 4°C. .. Plasmids were propagated using standard molecular biology techniques, and purified using QIAprep Spin Miniprep and HiSpeed Plasmid Maxi kits (Qiagen).

    Amplification:

    Article Title: Fisetin induces autophagic cell death through suppression of mTOR signaling pathway in prostate cancer cells
    Article Snippet: .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit (Qiagen, Valencia, CA). .. The non-targeting- and Beclin1-specific small interfering RNAs (siRNAs) were purchased from Dharmacon (Lafayette, CO) and cells were transfected using the nucleofection kit V specific for PC-3 transfection from Amaxa Biosystems (Gaithersburg, MD).

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
    Article Snippet: .. Amplified cDNA was isolated and purified using a HiSpeed Plasmid Maxi Kit (Qiagen; Valencia, CA) following the manufacturer's instructions and prepared for the biolistic transfection of organotypic hippocampal slice cultures. .. Biolistic transfection Cultures were transfected biolistically with a gene gun (Helios; Bio-Rad, Hercules, CA) as described .

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
    Article Snippet: Transfection studies were performed with plasmids containing the pcDNA3 vector backbone (Invitrogen) and a variety of cDNA sequences amplified from the LILRB1 cDNA clone with the use of platinumTaq polymerase (Invitrogen) and cloned with Kpn I and Not I sites included in the sense and antisense primers, respectively. .. All plasmids were confirmed by sequencing (Agencourt) and doubly purified from bacterial culture by HiSpeed Plasmid Maxi Kit (QIAGEN) followed by QIAquick PCR Purification Kit (QIAGEN) before transfection.

    Article Title: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB
    Article Snippet: Open reading frames of interest were amplified by PCR using Pfu DNA polymerase (Promega) prior to cloning into NanoBiT™ expression vectors with C- or N-terminal large or small BiT (Lg/SmBiT) fusions (pNB1k, pNB2k or pNB3k (Promega)). .. Plasmids were propagated using standard molecular biology techniques, and purified using QIAprep Spin Miniprep and HiSpeed Plasmid Maxi kits (Qiagen).

    Article Title: A plant flavonoid fisetin induces apoptosis in colon cancer cells by inhibition of COX2 and Wnt/EGFR/NF-?B-signaling pathways
    Article Snippet: .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit from Qiagen (Valencia, CA). .. Lipofectamine 2000 (Invitrogen) was used for transient transfection.

    Reporter Assay:

    Article Title: A plant flavonoid fisetin induces apoptosis in colon cancer cells by inhibition of COX2 and Wnt/EGFR/NF-?B-signaling pathways
    Article Snippet: Plasmids were amplified and purified using HiSpeed plasmid maxi kit from Qiagen (Valencia, CA). .. Vehicle or fisetin was added 1 day after the transfection for 24 h. Luciferase activities were determined using Dual Luciferase Reporter Assay System (Promega).

    Construct:

    Article Title: Bacteriophage C31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels
    Article Snippet: Permission to use HS4 insulator sequences and HS4 insulator constructs needs to be obtained from Dr. Gary Felsenfeld ( ) ( see ). .. Plasmid Purification Kits : Qiaprep Spin Miniprep Kit, HiSpeed Plasmid Maxi Kit (Qiagen, Valencia, CA).

    Article Title: Fisetin induces autophagic cell death through suppression of mTOR signaling pathway in prostate cancer cells
    Article Snippet: The bicistronic luciferase reporter construct was used to measure Cap-dependent translation. .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit (Qiagen, Valencia, CA).

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
    Article Snippet: Paragraph title: ABD and Rac constructs ... Amplified cDNA was isolated and purified using a HiSpeed Plasmid Maxi Kit (Qiagen; Valencia, CA) following the manufacturer's instructions and prepared for the biolistic transfection of organotypic hippocampal slice cultures.

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
    Article Snippet: Luciferase reporter constructs were generated by cloning LILRB1 promoter sequences into Nhe I and Xho I sites of pGL4.10 (Promega). .. All plasmids were confirmed by sequencing (Agencourt) and doubly purified from bacterial culture by HiSpeed Plasmid Maxi Kit (QIAGEN) followed by QIAquick PCR Purification Kit (QIAGEN) before transfection.

    Article Title: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB
    Article Snippet: With the exception of human SHIP-1, whereby human cell line (SUDHL4) cDNA was used as a template, in-house plasmid constructs were used as templates for PCR. .. Plasmids were propagated using standard molecular biology techniques, and purified using QIAprep Spin Miniprep and HiSpeed Plasmid Maxi kits (Qiagen).

    Article Title: A plant flavonoid fisetin induces apoptosis in colon cancer cells by inhibition of COX2 and Wnt/EGFR/NF-?B-signaling pathways
    Article Snippet: TOPflash plasmid construct was purchased from Millipore (Billerica, MA). .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit from Qiagen (Valencia, CA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: Cell death was also assessed by the internucleosomal degradation of genomic DNA using the Cell Death Detection ELISA Plus Kit (Roche Molecular Biochemicals). .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions.

    Incubation:

    Article Title: One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Article Snippet: .. An E. coli strain carrying each of these 25 pieces was inoculated into 150 ml of LB plus 12.5 μg/ml of chloramphenicol and 1X induction solution (Epicentre) and incubated at 37 °C for 16 h. The cultures were harvested, and the BACs were purified using a Qiagen HiSpeed Plasmid Maxi Kit. ..

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: Background values (incubation with buffer alone) were subtracted, and optical density values representing nucleosomal DNA fragments in treated samples were compared with those values obtained from the untreated control cells and expressed as the fold increase of oligonucleosomes. .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions.

    Luciferase:

    Article Title: Fisetin induces autophagic cell death through suppression of mTOR signaling pathway in prostate cancer cells
    Article Snippet: The bicistronic luciferase reporter construct was used to measure Cap-dependent translation. .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit (Qiagen, Valencia, CA).

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
    Article Snippet: Luciferase reporter constructs were generated by cloning LILRB1 promoter sequences into Nhe I and Xho I sites of pGL4.10 (Promega). .. All plasmids were confirmed by sequencing (Agencourt) and doubly purified from bacterial culture by HiSpeed Plasmid Maxi Kit (QIAGEN) followed by QIAquick PCR Purification Kit (QIAGEN) before transfection.

    Article Title: A plant flavonoid fisetin induces apoptosis in colon cancer cells by inhibition of COX2 and Wnt/EGFR/NF-?B-signaling pathways
    Article Snippet: Paragraph title: Plasmids, transfection and luciferase assay ... Plasmids were amplified and purified using HiSpeed plasmid maxi kit from Qiagen (Valencia, CA).

    Infection:

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions. .. 3T3-L1 preadipocytes (80% confluence) were lentivirus infected, selected with puromycin (2.5 μg/ml), and subjected to standard adipocyte differentiation.

    Expressing:

    Article Title: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB
    Article Snippet: Initial PCR products were cloned into pCR®-BluntII-TOPO® vectors using Zero Blunt TOPO PCR Cloning Kit (Invitrogen), sequenced and re-cloned into NanoBiT™ expression vectors with appropriate restriction enzyme sites, followed by ligation with T4 DNA ligase (Promega) overnight at 4°C. .. Plasmids were propagated using standard molecular biology techniques, and purified using QIAprep Spin Miniprep and HiSpeed Plasmid Maxi kits (Qiagen).

    Modification:

    Article Title: The Nucleoside Analog BMS-986001 Shows Greater In Vitro Activity against HIV-2 than against HIV-1
    Article Snippet: Transfection-grade (endonuclease-free) preparations of pROD9 and pNL4-3 were isolated using a HiSpeed plasmid maxikit with the buffers and reagents from an EndoFree plasmid maxikit (Qiagen, Valencia, CA). .. 293T/17 and MAGIC-5A cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Mediatech, Manassas, VA) supplemented with 4 mM l -glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco, Life Technologies Corp., Grand Island, NY) and with 10% fetal bovine serum (heat inactivated at 70°C for 30 min; HyClone, Logan, UT).

    Filtration:

    Article Title: Absolute cross section for low-energy-electron damage to condensed macromolecules: A case study of DNA
    Article Snippet: 1 968 966 amu per plasmid] was extracted from Esherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The tris(hydroxymethyl)aminomethane (Tris)—ethylenediaminetetraacetic acid (EDTA) (TE) buffer (10 mM/1 mM) was separated from DNA by gel filtration with a Sephadex G-50 medium.

    Article Title: Absolute cross-sections for DNA strand breaks and crosslinks induced by low energy electrons
    Article Snippet: 1 968 966 amu per plasmid] was extracted from Escherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The TE buffer (Tris–EDTA: 10 mM/1 mM) was separated from DNA by gel filtration with a Sephadex G-50 medium.

    Over Expression:

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: Lentiviral shRNA knockdown and cDNA overexpression MISSION lentiviral shRNA bacterial glycerol stocks for SNAP23, ATG9a, ATG7, ATG5, ATG16, and NM control plasmids were obtained from MilliporeSigma. .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions.

    Derivative Assay:

    Article Title: Characterisation of an engineered trastuzumab IgE antibody and effector cell mechanisms targeting HER2/neu-positive tumour cells
    Article Snippet: The cDNA derived from the protein sequences of the heavy and light chains of the trastuzumab variable regions was synthesised (Gene Art AG, Regensburg, Germany) based on the published protein sequence of trastuzumab (source: ; 1n8z) [ ]. .. For transfection into compatible HEK293 cells, vector DNA was produced using the HiSpeed Plasmid Maxi Kit® (Qiagen® ), according to the manufacturer's instructions.

    High Performance Liquid Chromatography:

    Article Title: Characterisation of an engineered trastuzumab IgE antibody and effector cell mechanisms targeting HER2/neu-positive tumour cells
    Article Snippet: For transfection into compatible HEK293 cells, vector DNA was produced using the HiSpeed Plasmid Maxi Kit® (Qiagen® ), according to the manufacturer's instructions. .. Antibody purity was confirmed by HPLC analysis.

    Transfection:

    Article Title: The Nucleoside Analog BMS-986001 Shows Greater In Vitro Activity against HIV-2 than against HIV-1
    Article Snippet: .. Transfection-grade (endonuclease-free) preparations of pROD9 and pNL4-3 were isolated using a HiSpeed plasmid maxikit with the buffers and reagents from an EndoFree plasmid maxikit (Qiagen, Valencia, CA). .. 293tsA1609neo clone 17 (293T/17) cells were purchased from the American Type Culture Collection (Manassas, VA).

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions. .. 3T3-L1 preadipocytes (80% confluence) were lentivirus infected, selected with puromycin (2.5 μg/ml), and subjected to standard adipocyte differentiation.

    Article Title: Caspases cleave and inhibit the microRNA processing protein DiGeorge Critical Region 8
    Article Snippet: Paragraph title: Cell cultures and transfection ... Plasmids were purified using the HiSpeed Maxi Plasmid Kit (Qiagen, Valencia, CA).

    Article Title: Fisetin induces autophagic cell death through suppression of mTOR signaling pathway in prostate cancer cells
    Article Snippet: Paragraph title: Plasmids, siRNA and transfection ... Plasmids were amplified and purified using HiSpeed plasmid maxi kit (Qiagen, Valencia, CA).

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
    Article Snippet: .. Amplified cDNA was isolated and purified using a HiSpeed Plasmid Maxi Kit (Qiagen; Valencia, CA) following the manufacturer's instructions and prepared for the biolistic transfection of organotypic hippocampal slice cultures. .. Biolistic transfection Cultures were transfected biolistically with a gene gun (Helios; Bio-Rad, Hercules, CA) as described .

    Article Title: Characterisation of an engineered trastuzumab IgE antibody and effector cell mechanisms targeting HER2/neu-positive tumour cells
    Article Snippet: .. For transfection into compatible HEK293 cells, vector DNA was produced using the HiSpeed Plasmid Maxi Kit® (Qiagen® ), according to the manufacturer's instructions. .. HEK293 cells were harvested and seeded at 4 × 105 cells/mL and allowed to adhere before being transfected with 1 μg of DNA with the aid of 2 μg of PEI (Polyethenylenimine, MW: 25 kDa; Polysciences Inc., Warrington, PA, USA) per 4 × 107 cells [ ].

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
    Article Snippet: .. All plasmids were confirmed by sequencing (Agencourt) and doubly purified from bacterial culture by HiSpeed Plasmid Maxi Kit (QIAGEN) followed by QIAquick PCR Purification Kit (QIAGEN) before transfection. .. Freshly isolated PBMCs or AutoMACS-purified monocytes were transfected with the use of the Nucleofector II (Lonza) as described by the manufacturer.

    Article Title: A plant flavonoid fisetin induces apoptosis in colon cancer cells by inhibition of COX2 and Wnt/EGFR/NF-?B-signaling pathways
    Article Snippet: Paragraph title: Plasmids, transfection and luciferase assay ... Plasmids were amplified and purified using HiSpeed plasmid maxi kit from Qiagen (Valencia, CA).

    Ligation:

    Article Title: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB
    Article Snippet: Initial PCR products were cloned into pCR®-BluntII-TOPO® vectors using Zero Blunt TOPO PCR Cloning Kit (Invitrogen), sequenced and re-cloned into NanoBiT™ expression vectors with appropriate restriction enzyme sites, followed by ligation with T4 DNA ligase (Promega) overnight at 4°C. .. Plasmids were propagated using standard molecular biology techniques, and purified using QIAprep Spin Miniprep and HiSpeed Plasmid Maxi kits (Qiagen).

    Cell Culture:

    Article Title: The Nucleoside Analog BMS-986001 Shows Greater In Vitro Activity against HIV-2 than against HIV-1
    Article Snippet: Transfection-grade (endonuclease-free) preparations of pROD9 and pNL4-3 were isolated using a HiSpeed plasmid maxikit with the buffers and reagents from an EndoFree plasmid maxikit (Qiagen, Valencia, CA). .. 293T/17 and MAGIC-5A cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Mediatech, Manassas, VA) supplemented with 4 mM l -glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin (Gibco, Life Technologies Corp., Grand Island, NY) and with 10% fetal bovine serum (heat inactivated at 70°C for 30 min; HyClone, Logan, UT).

    Generated:

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
    Article Snippet: Luciferase reporter constructs were generated by cloning LILRB1 promoter sequences into Nhe I and Xho I sites of pGL4.10 (Promega). .. All plasmids were confirmed by sequencing (Agencourt) and doubly purified from bacterial culture by HiSpeed Plasmid Maxi Kit (QIAGEN) followed by QIAquick PCR Purification Kit (QIAGEN) before transfection.

    Sequencing:

    Article Title: Characterisation of an engineered trastuzumab IgE antibody and effector cell mechanisms targeting HER2/neu-positive tumour cells
    Article Snippet: The cDNA derived from the protein sequences of the heavy and light chains of the trastuzumab variable regions was synthesised (Gene Art AG, Regensburg, Germany) based on the published protein sequence of trastuzumab (source: ; 1n8z) [ ]. .. For transfection into compatible HEK293 cells, vector DNA was produced using the HiSpeed Plasmid Maxi Kit® (Qiagen® ), according to the manufacturer's instructions.

    Article Title: Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
    Article Snippet: tRNA purification The gene encoding M. maripaludis tRNASec with the preceding sequence of the T7 promoter was cloned into the pUC18 plasmid, and the gene encoding M. maripaludis tRNASer UGA with the preceding sequence of the T7 promoter was cloned into the pUC19 plasmid. .. Plasmid DNA was purified using the HISpeed Plasmid Maxi kit (Qiagen).

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
    Article Snippet: .. All plasmids were confirmed by sequencing (Agencourt) and doubly purified from bacterial culture by HiSpeed Plasmid Maxi Kit (QIAGEN) followed by QIAquick PCR Purification Kit (QIAGEN) before transfection. .. Freshly isolated PBMCs or AutoMACS-purified monocytes were transfected with the use of the Nucleofector II (Lonza) as described by the manufacturer.

    Article Title: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB
    Article Snippet: Plasmids were propagated using standard molecular biology techniques, and purified using QIAprep Spin Miniprep and HiSpeed Plasmid Maxi kits (Qiagen). .. Mutation of the CD32B ITIM was achieved via site-directed mutagenesis using the Quikchange Lightning Multi Site-Directed Mutagenesis Kit (Agilent), according to the manufacturers’ instructions, and confirmed by sequencing.

    Nucleic Acid Electrophoresis:

    Article Title: One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Article Snippet: An E. coli strain carrying each of these 25 pieces was inoculated into 150 ml of LB plus 12.5 μg/ml of chloramphenicol and 1X induction solution (Epicentre) and incubated at 37 °C for 16 h. The cultures were harvested, and the BACs were purified using a Qiagen HiSpeed Plasmid Maxi Kit. .. The products were dissolved in TE buffer to a final concentration of 120 ng/μl, as determined by gel electrophoresis with standards.

    Mutagenesis:

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
    Article Snippet: Mutation of sequences within LILRB1 exon 1 was performed on pcDNA3- LILRB1 full-length plasmid with the use of the QuikChange II XL site-directed mutagenesis kit, as described by the manufacturer (Stratagene). .. All plasmids were confirmed by sequencing (Agencourt) and doubly purified from bacterial culture by HiSpeed Plasmid Maxi Kit (QIAGEN) followed by QIAquick PCR Purification Kit (QIAGEN) before transfection.

    Article Title: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB
    Article Snippet: Plasmids were propagated using standard molecular biology techniques, and purified using QIAprep Spin Miniprep and HiSpeed Plasmid Maxi kits (Qiagen). .. Mutation of the CD32B ITIM was achieved via site-directed mutagenesis using the Quikchange Lightning Multi Site-Directed Mutagenesis Kit (Agilent), according to the manufacturers’ instructions, and confirmed by sequencing.

    Article Title: A plant flavonoid fisetin induces apoptosis in colon cancer cells by inhibition of COX2 and Wnt/EGFR/NF-?B-signaling pathways
    Article Snippet: The pGL3 vector-based COX2 luciferase constructs wild-type (WT) and mutant (MT) that does not contain TCF-binding element were generous gifts from Dr S.Perwez Hussain, Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD. .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit from Qiagen (Valencia, CA).

    Isolation:

    Article Title: The Nucleoside Analog BMS-986001 Shows Greater In Vitro Activity against HIV-2 than against HIV-1
    Article Snippet: .. Transfection-grade (endonuclease-free) preparations of pROD9 and pNL4-3 were isolated using a HiSpeed plasmid maxikit with the buffers and reagents from an EndoFree plasmid maxikit (Qiagen, Valencia, CA). .. 293tsA1609neo clone 17 (293T/17) cells were purchased from the American Type Culture Collection (Manassas, VA).

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
    Article Snippet: .. Amplified cDNA was isolated and purified using a HiSpeed Plasmid Maxi Kit (Qiagen; Valencia, CA) following the manufacturer's instructions and prepared for the biolistic transfection of organotypic hippocampal slice cultures. .. Biolistic transfection Cultures were transfected biolistically with a gene gun (Helios; Bio-Rad, Hercules, CA) as described .

    Microscopy:

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: Plasma membrane permeability was determined by incubating cells with 250 ng/ml PI and 5 μg/ml Hoechst 33342 for 10 minutes and then analyzing them under a microscope. .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions.

    Purification:

    Article Title: Bacteriophage C31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels
    Article Snippet: .. Plasmid Purification Kits : Qiaprep Spin Miniprep Kit, HiSpeed Plasmid Maxi Kit (Qiagen, Valencia, CA). .. In vitro RNA transcription : T7 mMessage machine (Ambion, Austin, TX).

    Article Title: One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Article Snippet: .. An E. coli strain carrying each of these 25 pieces was inoculated into 150 ml of LB plus 12.5 μg/ml of chloramphenicol and 1X induction solution (Epicentre) and incubated at 37 °C for 16 h. The cultures were harvested, and the BACs were purified using a Qiagen HiSpeed Plasmid Maxi Kit. ..

    Article Title: The Nucleoside Analog BMS-986001 Shows Greater In Vitro Activity against HIV-2 than against HIV-1
    Article Snippet: Bacterial culture steps (i.e., single-colony isolation, starter cultures, and large-scale expansions for plasmid purification) were performed at 30°C to minimize the outgrowth of plasmids with deletions in the HIV insert ( ). .. Transfection-grade (endonuclease-free) preparations of pROD9 and pNL4-3 were isolated using a HiSpeed plasmid maxikit with the buffers and reagents from an EndoFree plasmid maxikit (Qiagen, Valencia, CA).

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions. .. 3T3-L1 preadipocytes (80% confluence) were lentivirus infected, selected with puromycin (2.5 μg/ml), and subjected to standard adipocyte differentiation.

    Article Title: Caspases cleave and inhibit the microRNA processing protein DiGeorge Critical Region 8
    Article Snippet: .. Plasmids were purified using the HiSpeed Maxi Plasmid Kit (Qiagen, Valencia, CA). .. HeLa cells were washed twice with PBS and collected via centrifugation at 4°C.

    Article Title: Fisetin induces autophagic cell death through suppression of mTOR signaling pathway in prostate cancer cells
    Article Snippet: .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit (Qiagen, Valencia, CA). .. The non-targeting- and Beclin1-specific small interfering RNAs (siRNAs) were purchased from Dharmacon (Lafayette, CO) and cells were transfected using the nucleofection kit V specific for PC-3 transfection from Amaxa Biosystems (Gaithersburg, MD).

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
    Article Snippet: .. Amplified cDNA was isolated and purified using a HiSpeed Plasmid Maxi Kit (Qiagen; Valencia, CA) following the manufacturer's instructions and prepared for the biolistic transfection of organotypic hippocampal slice cultures. .. Biolistic transfection Cultures were transfected biolistically with a gene gun (Helios; Bio-Rad, Hercules, CA) as described .

    Article Title: Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
    Article Snippet: .. Plasmid DNA was purified using the HISpeed Plasmid Maxi kit (Qiagen). .. The purified plasmid was digested with BstNI for run-off transcription as described previously ( ).

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
    Article Snippet: .. All plasmids were confirmed by sequencing (Agencourt) and doubly purified from bacterial culture by HiSpeed Plasmid Maxi Kit (QIAGEN) followed by QIAquick PCR Purification Kit (QIAGEN) before transfection. .. Freshly isolated PBMCs or AutoMACS-purified monocytes were transfected with the use of the Nucleofector II (Lonza) as described by the manufacturer.

    Article Title: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB
    Article Snippet: .. Plasmids were propagated using standard molecular biology techniques, and purified using QIAprep Spin Miniprep and HiSpeed Plasmid Maxi kits (Qiagen). .. Mutation of the CD32B ITIM was achieved via site-directed mutagenesis using the Quikchange Lightning Multi Site-Directed Mutagenesis Kit (Agilent), according to the manufacturers’ instructions, and confirmed by sequencing.

    Article Title: A plant flavonoid fisetin induces apoptosis in colon cancer cells by inhibition of COX2 and Wnt/EGFR/NF-?B-signaling pathways
    Article Snippet: .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit from Qiagen (Valencia, CA). .. Lipofectamine 2000 (Invitrogen) was used for transient transfection.

    Article Title: Absolute cross section for low-energy-electron damage to condensed macromolecules: A case study of DNA
    Article Snippet: .. 1 968 966 amu per plasmid] was extracted from Esherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The purified plasmid DNA consisted of 97% supercoiled, 2% concatemeric, and 1% nicked circular forms.

    Article Title: Absolute cross-sections for DNA strand breaks and crosslinks induced by low energy electrons
    Article Snippet: .. 1 968 966 amu per plasmid] was extracted from Escherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The purified plasmid consisted of 96% supercoiled, 1% concatemeric, 1% nicked circular and 2% crosslink forms.

    Polymerase Chain Reaction:

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
    Article Snippet: .. All plasmids were confirmed by sequencing (Agencourt) and doubly purified from bacterial culture by HiSpeed Plasmid Maxi Kit (QIAGEN) followed by QIAquick PCR Purification Kit (QIAGEN) before transfection. .. Freshly isolated PBMCs or AutoMACS-purified monocytes were transfected with the use of the Nucleofector II (Lonza) as described by the manufacturer.

    Article Title: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB
    Article Snippet: Initial PCR products were cloned into pCR®-BluntII-TOPO® vectors using Zero Blunt TOPO PCR Cloning Kit (Invitrogen), sequenced and re-cloned into NanoBiT™ expression vectors with appropriate restriction enzyme sites, followed by ligation with T4 DNA ligase (Promega) overnight at 4°C. .. Plasmids were propagated using standard molecular biology techniques, and purified using QIAprep Spin Miniprep and HiSpeed Plasmid Maxi kits (Qiagen).

    Plasmid Preparation:

    Article Title: Bacteriophage C31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels
    Article Snippet: .. Plasmid Purification Kits : Qiaprep Spin Miniprep Kit, HiSpeed Plasmid Maxi Kit (Qiagen, Valencia, CA). .. In vitro RNA transcription : T7 mMessage machine (Ambion, Austin, TX).

    Article Title: One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Article Snippet: .. An E. coli strain carrying each of these 25 pieces was inoculated into 150 ml of LB plus 12.5 μg/ml of chloramphenicol and 1X induction solution (Epicentre) and incubated at 37 °C for 16 h. The cultures were harvested, and the BACs were purified using a Qiagen HiSpeed Plasmid Maxi Kit. ..

    Article Title: The Nucleoside Analog BMS-986001 Shows Greater In Vitro Activity against HIV-2 than against HIV-1
    Article Snippet: .. Transfection-grade (endonuclease-free) preparations of pROD9 and pNL4-3 were isolated using a HiSpeed plasmid maxikit with the buffers and reagents from an EndoFree plasmid maxikit (Qiagen, Valencia, CA). .. 293tsA1609neo clone 17 (293T/17) cells were purchased from the American Type Culture Collection (Manassas, VA).

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions. .. 3T3-L1 preadipocytes (80% confluence) were lentivirus infected, selected with puromycin (2.5 μg/ml), and subjected to standard adipocyte differentiation.

    Article Title: Caspases cleave and inhibit the microRNA processing protein DiGeorge Critical Region 8
    Article Snippet: .. Plasmids were purified using the HiSpeed Maxi Plasmid Kit (Qiagen, Valencia, CA). .. HeLa cells were washed twice with PBS and collected via centrifugation at 4°C.

    Article Title: Fisetin induces autophagic cell death through suppression of mTOR signaling pathway in prostate cancer cells
    Article Snippet: .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit (Qiagen, Valencia, CA). .. The non-targeting- and Beclin1-specific small interfering RNAs (siRNAs) were purchased from Dharmacon (Lafayette, CO) and cells were transfected using the nucleofection kit V specific for PC-3 transfection from Amaxa Biosystems (Gaithersburg, MD).

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
    Article Snippet: .. Amplified cDNA was isolated and purified using a HiSpeed Plasmid Maxi Kit (Qiagen; Valencia, CA) following the manufacturer's instructions and prepared for the biolistic transfection of organotypic hippocampal slice cultures. .. Biolistic transfection Cultures were transfected biolistically with a gene gun (Helios; Bio-Rad, Hercules, CA) as described .

    Article Title: Characterisation of an engineered trastuzumab IgE antibody and effector cell mechanisms targeting HER2/neu-positive tumour cells
    Article Snippet: .. For transfection into compatible HEK293 cells, vector DNA was produced using the HiSpeed Plasmid Maxi Kit® (Qiagen® ), according to the manufacturer's instructions. .. HEK293 cells were harvested and seeded at 4 × 105 cells/mL and allowed to adhere before being transfected with 1 μg of DNA with the aid of 2 μg of PEI (Polyethenylenimine, MW: 25 kDa; Polysciences Inc., Warrington, PA, USA) per 4 × 107 cells [ ].

    Article Title: Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
    Article Snippet: .. Plasmid DNA was purified using the HISpeed Plasmid Maxi kit (Qiagen). .. The purified plasmid was digested with BstNI for run-off transcription as described previously ( ).

    Article Title: Promoter choice and translational repression determine cell type-specific cell surface density of the inhibitory receptor CD85j expressed on different hematopoietic lineages
    Article Snippet: .. All plasmids were confirmed by sequencing (Agencourt) and doubly purified from bacterial culture by HiSpeed Plasmid Maxi Kit (QIAGEN) followed by QIAquick PCR Purification Kit (QIAGEN) before transfection. .. Freshly isolated PBMCs or AutoMACS-purified monocytes were transfected with the use of the Nucleofector II (Lonza) as described by the manufacturer.

    Article Title: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB
    Article Snippet: .. Plasmids were propagated using standard molecular biology techniques, and purified using QIAprep Spin Miniprep and HiSpeed Plasmid Maxi kits (Qiagen). .. Mutation of the CD32B ITIM was achieved via site-directed mutagenesis using the Quikchange Lightning Multi Site-Directed Mutagenesis Kit (Agilent), according to the manufacturers’ instructions, and confirmed by sequencing.

    Article Title: A plant flavonoid fisetin induces apoptosis in colon cancer cells by inhibition of COX2 and Wnt/EGFR/NF-?B-signaling pathways
    Article Snippet: .. Plasmids were amplified and purified using HiSpeed plasmid maxi kit from Qiagen (Valencia, CA). .. Lipofectamine 2000 (Invitrogen) was used for transient transfection.

    Article Title: Absolute cross section for low-energy-electron damage to condensed macromolecules: A case study of DNA
    Article Snippet: .. 1 968 966 amu per plasmid] was extracted from Esherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The purified plasmid DNA consisted of 97% supercoiled, 2% concatemeric, and 1% nicked circular forms.

    Article Title: Absolute cross-sections for DNA strand breaks and crosslinks induced by low energy electrons
    Article Snippet: .. 1 968 966 amu per plasmid] was extracted from Escherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The purified plasmid consisted of 96% supercoiled, 1% concatemeric, 1% nicked circular and 2% crosslink forms.

    Dominant Negative Mutation:

    Article Title: The Actin Binding Domain of ?I-Spectrin Regulates the Morphological and Functional Dynamics of Dendritic Spines
    Article Snippet: GFP-actin was from Clontech (Mountain View, CA). cDNAs encoding wild type and dominant negative Rac 1 and 3 were myc- and HA-tagged constructs from the Guthrie cDNA Resource Center (Rollo, MO). .. Amplified cDNA was isolated and purified using a HiSpeed Plasmid Maxi Kit (Qiagen; Valencia, CA) following the manufacturer's instructions and prepared for the biolistic transfection of organotypic hippocampal slice cultures.

    Irradiation:

    Article Title: Absolute cross section for low-energy-electron damage to condensed macromolecules: A case study of DNA
    Article Snippet: The experimental details of sample preparation, irradiation, and postirradiation analysis techniques employed in the present studies have been reported in detail elsewhere [ , ]. .. 1 968 966 amu per plasmid] was extracted from Esherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN).

    Article Title: Absolute cross-sections for DNA strand breaks and crosslinks induced by low energy electrons
    Article Snippet: The experimental details of sample preparation, irradiation, and post-irradiation analysis techniques employed in the present studies have been reported in detail elsewhere., , Here, we provide a brief description of the most pertinent elements. .. 1 968 966 amu per plasmid] was extracted from Escherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN).

    shRNA:

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions. .. 3T3-L1 preadipocytes (80% confluence) were lentivirus infected, selected with puromycin (2.5 μg/ml), and subjected to standard adipocyte differentiation.

    Sample Prep:

    Article Title: Absolute cross section for low-energy-electron damage to condensed macromolecules: A case study of DNA
    Article Snippet: The experimental details of sample preparation, irradiation, and postirradiation analysis techniques employed in the present studies have been reported in detail elsewhere [ , ]. .. 1 968 966 amu per plasmid] was extracted from Esherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN).

    Article Title: Absolute cross-sections for DNA strand breaks and crosslinks induced by low energy electrons
    Article Snippet: The experimental details of sample preparation, irradiation, and post-irradiation analysis techniques employed in the present studies have been reported in detail elsewhere., , Here, we provide a brief description of the most pertinent elements. .. 1 968 966 amu per plasmid] was extracted from Escherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN).

    In Vitro:

    Article Title: Bacteriophage C31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels
    Article Snippet: Plasmid Purification Kits : Qiaprep Spin Miniprep Kit, HiSpeed Plasmid Maxi Kit (Qiagen, Valencia, CA). .. In vitro RNA transcription : T7 mMessage machine (Ambion, Austin, TX).

    Electrophoresis:

    Article Title: Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
    Article Snippet: Plasmid DNA was purified using the HISpeed Plasmid Maxi kit (Qiagen). .. The transcript was phenol–chloroform extracted, ethanol precipitated and purified by electrophoresis on a 12% denaturing polyacrylamide gel.

    Ethanol Precipitation:

    Article Title: One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Article Snippet: An E. coli strain carrying each of these 25 pieces was inoculated into 150 ml of LB plus 12.5 μg/ml of chloramphenicol and 1X induction solution (Epicentre) and incubated at 37 °C for 16 h. The cultures were harvested, and the BACs were purified using a Qiagen HiSpeed Plasmid Maxi Kit. .. Each insert was excised by restriction digestion at the recommended temperature for 16 h and then terminated by phenol-chloroform extraction and ethanol precipitation.

    Spectrophotometry:

    Article Title: Absolute cross section for low-energy-electron damage to condensed macromolecules: A case study of DNA
    Article Snippet: 1 968 966 amu per plasmid] was extracted from Esherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The concentration of DNA and the relative quantity of proteins in the plasmid DNA solution was then calculated by measuring the ratio of ultraviolet (UV) absorption of DNA and protein at 260 and 280 nm, respectively, with a Synergy HT-I spectrophotometer.

    Article Title: Absolute cross-sections for DNA strand breaks and crosslinks induced by low energy electrons
    Article Snippet: 1 968 966 amu per plasmid] was extracted from Escherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The concentration of DNA relative to the quantity of proteins in the plasmid solution was calculated by measuring the absorption ratio of 260 nm and 280 nm light with a spectrophotometer (BioTek, Epoch).

    Produced:

    Article Title: Characterisation of an engineered trastuzumab IgE antibody and effector cell mechanisms targeting HER2/neu-positive tumour cells
    Article Snippet: .. For transfection into compatible HEK293 cells, vector DNA was produced using the HiSpeed Plasmid Maxi Kit® (Qiagen® ), according to the manufacturer's instructions. .. HEK293 cells were harvested and seeded at 4 × 105 cells/mL and allowed to adhere before being transfected with 1 μg of DNA with the aid of 2 μg of PEI (Polyethenylenimine, MW: 25 kDa; Polysciences Inc., Warrington, PA, USA) per 4 × 107 cells [ ].

    Concentration Assay:

    Article Title: One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
    Article Snippet: An E. coli strain carrying each of these 25 pieces was inoculated into 150 ml of LB plus 12.5 μg/ml of chloramphenicol and 1X induction solution (Epicentre) and incubated at 37 °C for 16 h. The cultures were harvested, and the BACs were purified using a Qiagen HiSpeed Plasmid Maxi Kit. .. The products were dissolved in TE buffer to a final concentration of 120 ng/μl, as determined by gel electrophoresis with standards.

    Article Title: Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
    Article Snippet: Plasmid DNA was purified using the HISpeed Plasmid Maxi kit (Qiagen). .. The tRNA transcripts were refolded at a concentration of 10 µM by heating for 5 min at 70°C in buffer containing 10 mM Tris–HCl (pH 7.0), followed by addition of 5 mM MgCl2 and immediate cooling on ice ( ).

    Article Title: Absolute cross section for low-energy-electron damage to condensed macromolecules: A case study of DNA
    Article Snippet: 1 968 966 amu per plasmid] was extracted from Esherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The concentration of DNA and the relative quantity of proteins in the plasmid DNA solution was then calculated by measuring the ratio of ultraviolet (UV) absorption of DNA and protein at 260 and 280 nm, respectively, with a Synergy HT-I spectrophotometer.

    Article Title: Absolute cross-sections for DNA strand breaks and crosslinks induced by low energy electrons
    Article Snippet: 1 968 966 amu per plasmid] was extracted from Escherichia coli JM109 and purified with a HiSpeed plasmid Maxi kit (QIAGEN). .. The concentration of DNA relative to the quantity of proteins in the plasmid solution was calculated by measuring the absorption ratio of 260 nm and 280 nm light with a spectrophotometer (BioTek, Epoch).

    Permeability:

    Article Title: SNAP23 regulates BAX-dependent adipocyte programmed cell death independently of canonical macroautophagy
    Article Snippet: Plasma membrane permeability was determined by incubating cells with 250 ng/ml PI and 5 μg/ml Hoechst 33342 for 10 minutes and then analyzing them under a microscope. .. The plasmid DNAs were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN) and transfected into HEK293T cells along with Lentiviral Packaging Mix (MilliporeSigma) to produce lentivirus packed with shRNA and cDNA according to the manufacturer’s instructions.

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    Qiagen hispeed plasmid maxi prep kit
    Hispeed Plasmid Maxi Prep Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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