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Illumina Inc hiseq single end 50 bp sequencing
Hiseq Single End 50 Bp Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hiseq single end 50 bp sequencing/product/Illumina Inc
Average 89 stars, based on 3 article reviews
Price from $9.99 to $1999.99
hiseq single end 50 bp sequencing - by Bioz Stars, 2020-02
89/100 stars

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Related Articles

DNA Extraction:

Article Title: CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo
Article Snippet: Genomic DNA extraction and sequencing library preparation was performed as described previously , with minor modifications - MyTaq (Bioline) was used for PCR amplification of adapter ligated DNA. .. Libraries were sequenced using Illumina HiSeq single-end 50 bp sequencing.

Amplification:

Article Title: CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo
Article Snippet: Genomic DNA extraction and sequencing library preparation was performed as described previously , with minor modifications - MyTaq (Bioline) was used for PCR amplification of adapter ligated DNA. .. Libraries were sequenced using Illumina HiSeq single-end 50 bp sequencing.

Modification:

Article Title: CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo
Article Snippet: Libraries were sequenced using Illumina HiSeq single-end 50 bp sequencing. .. Sequencing data were mapped back to release 6.03 of the Drosophila genome using a previously described pipeline, which was modified to output Dam-only datafiles ( ) (available at https://github.com/tonysouthall/damidseq_pipeline_output_Dam-only_data [ ; copy archived at https://github.com/elifesciences-publications/damidseq_pipeline_output_Dam-only_data ]).

Sequencing:

Article Title: CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo
Article Snippet: .. Libraries were sequenced using Illumina HiSeq single-end 50 bp sequencing. ..

Polymerase Chain Reaction:

Article Title: CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo
Article Snippet: Genomic DNA extraction and sequencing library preparation was performed as described previously , with minor modifications - MyTaq (Bioline) was used for PCR amplification of adapter ligated DNA. .. Libraries were sequenced using Illumina HiSeq single-end 50 bp sequencing.

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  • 99
    Illumina Inc hiseq 4000 system
    Sequencing data collection and analysis workflow. Two weeks prior to mate-pairing with A v y / a males, 8- to 10-wk-old wild-type a / a dams were placed on one of two experimental diet groups: (1) Control (modified, phytoestrogen-free 7% corn oil AIN-93G), or (2) Control + 50 μ g BPA / kg diet. Dietary exposure was continued through gestation and lactation until weaning at postnatal day 21. Genomic DNA was isolated from matched wild-type a / a offspring blood samples at 2, 4, and 10 months of age. DNA was isolated from a subset of Control ( n = 6 per age group) and BPA-exposed ( n = 6 per age group) mice, then processed in preparation for HsMeDIP-seq. All processed samples were amplified and sequenced on an Illumina <t>HiSeq</t> 4000 sequencer using single-end, 50 nt reads. BPA-related differentially hydroxymethylated regions (DHMRs) were identified and annotated using a bioinformatics pipeline. Annotated DHMRs were then visualized in the genome browser. The target gene region—Gnas—was then validated using RT-qPCR on available RNA from the blood samples.
    Hiseq 4000 System, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 4000 system/product/Illumina Inc
    Average 99 stars, based on 167 article reviews
    Price from $9.99 to $1999.99
    hiseq 4000 system - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    99
    Illumina Inc hiseq 2000 platform
    Sequencing data collection and analysis workflow. Two weeks prior to mate-pairing with A v y / a males, 8- to 10-wk-old wild-type a / a dams were placed on one of two experimental diet groups: (1) Control (modified, phytoestrogen-free 7% corn oil AIN-93G), or (2) Control + 50 μ g BPA / kg diet. Dietary exposure was continued through gestation and lactation until weaning at postnatal day 21. Genomic DNA was isolated from matched wild-type a / a offspring blood samples at 2, 4, and 10 months of age. DNA was isolated from a subset of Control ( n = 6 per age group) and BPA-exposed ( n = 6 per age group) mice, then processed in preparation for HsMeDIP-seq. All processed samples were amplified and sequenced on an Illumina <t>HiSeq</t> 4000 sequencer using single-end, 50 nt reads. BPA-related differentially hydroxymethylated regions (DHMRs) were identified and annotated using a bioinformatics pipeline. Annotated DHMRs were then visualized in the genome browser. The target gene region—Gnas—was then validated using RT-qPCR on available RNA from the blood samples.
    Hiseq 2000 Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3759 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 platform/product/Illumina Inc
    Average 99 stars, based on 3759 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 platform - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    Image Search Results


    Sequencing data collection and analysis workflow. Two weeks prior to mate-pairing with A v y / a males, 8- to 10-wk-old wild-type a / a dams were placed on one of two experimental diet groups: (1) Control (modified, phytoestrogen-free 7% corn oil AIN-93G), or (2) Control + 50 μ g BPA / kg diet. Dietary exposure was continued through gestation and lactation until weaning at postnatal day 21. Genomic DNA was isolated from matched wild-type a / a offspring blood samples at 2, 4, and 10 months of age. DNA was isolated from a subset of Control ( n = 6 per age group) and BPA-exposed ( n = 6 per age group) mice, then processed in preparation for HsMeDIP-seq. All processed samples were amplified and sequenced on an Illumina HiSeq 4000 sequencer using single-end, 50 nt reads. BPA-related differentially hydroxymethylated regions (DHMRs) were identified and annotated using a bioinformatics pipeline. Annotated DHMRs were then visualized in the genome browser. The target gene region—Gnas—was then validated using RT-qPCR on available RNA from the blood samples.

    Journal: Environmental Health Perspectives

    Article Title: Longitudinal Effects of Developmental Bisphenol A Exposure on Epigenome-Wide DNA Hydroxymethylation at Imprinted Loci in Mouse Blood

    doi: 10.1289/EHP3441

    Figure Lengend Snippet: Sequencing data collection and analysis workflow. Two weeks prior to mate-pairing with A v y / a males, 8- to 10-wk-old wild-type a / a dams were placed on one of two experimental diet groups: (1) Control (modified, phytoestrogen-free 7% corn oil AIN-93G), or (2) Control + 50 μ g BPA / kg diet. Dietary exposure was continued through gestation and lactation until weaning at postnatal day 21. Genomic DNA was isolated from matched wild-type a / a offspring blood samples at 2, 4, and 10 months of age. DNA was isolated from a subset of Control ( n = 6 per age group) and BPA-exposed ( n = 6 per age group) mice, then processed in preparation for HsMeDIP-seq. All processed samples were amplified and sequenced on an Illumina HiSeq 4000 sequencer using single-end, 50 nt reads. BPA-related differentially hydroxymethylated regions (DHMRs) were identified and annotated using a bioinformatics pipeline. Annotated DHMRs were then visualized in the genome browser. The target gene region—Gnas—was then validated using RT-qPCR on available RNA from the blood samples.

    Article Snippet: Single-end, 50 -bp reads were obtained for each library by sequencing on the HiSeq 4000 system (Illumina).

    Techniques: Sequencing, Modification, Isolation, Mouse Assay, Amplification, Quantitative RT-PCR