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Illumina Inc hiseq 2000 sequencer
Performance summary of mRRBS . Ninety-six samples were processed using mRRBS and sequenced with eight lanes of Illumina <t>HiSeq</t> 2000 using 12 barcoded adapters per lane. (a) The total number of reads for each sample is shown 84 samples with > 5 million total reads were included in the subsequent comparisons. (b) Quartile plots of summary coverage depth from these samples. The minimum and maximum values are bounded by the light blue area in (b-d), while the darker blue area represents the interquartile range. The dark blue line indicates the median. (c,d) MspI in silico digestion of the hg19 genome produced a total of 1,124,739 fragments. (c) The percentage of fragments of each fragment size that were covered by at least one read. (d) The average coverage depth for fragments of each length. Genomic MspI-digested fragments longer than 300 bp were not included in the sequence alignment target, which partly contributes to the sharp drop in coverage at 300 bp in (c,d).
Hiseq 2000 Sequencer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 1047 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hiseq 2000 sequencer/product/Illumina Inc
Average 92 stars, based on 1047 article reviews
Price from $9.99 to $1999.99
hiseq 2000 sequencer - by Bioz Stars, 2020-05
92/100 stars

Images

1) Product Images from "Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling"

Article Title: Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling

Journal: Genome Biology

doi: 10.1186/gb-2012-13-10-r92

Performance summary of mRRBS . Ninety-six samples were processed using mRRBS and sequenced with eight lanes of Illumina HiSeq 2000 using 12 barcoded adapters per lane. (a) The total number of reads for each sample is shown 84 samples with > 5 million total reads were included in the subsequent comparisons. (b) Quartile plots of summary coverage depth from these samples. The minimum and maximum values are bounded by the light blue area in (b-d), while the darker blue area represents the interquartile range. The dark blue line indicates the median. (c,d) MspI in silico digestion of the hg19 genome produced a total of 1,124,739 fragments. (c) The percentage of fragments of each fragment size that were covered by at least one read. (d) The average coverage depth for fragments of each length. Genomic MspI-digested fragments longer than 300 bp were not included in the sequence alignment target, which partly contributes to the sharp drop in coverage at 300 bp in (c,d).
Figure Legend Snippet: Performance summary of mRRBS . Ninety-six samples were processed using mRRBS and sequenced with eight lanes of Illumina HiSeq 2000 using 12 barcoded adapters per lane. (a) The total number of reads for each sample is shown 84 samples with > 5 million total reads were included in the subsequent comparisons. (b) Quartile plots of summary coverage depth from these samples. The minimum and maximum values are bounded by the light blue area in (b-d), while the darker blue area represents the interquartile range. The dark blue line indicates the median. (c,d) MspI in silico digestion of the hg19 genome produced a total of 1,124,739 fragments. (c) The percentage of fragments of each fragment size that were covered by at least one read. (d) The average coverage depth for fragments of each length. Genomic MspI-digested fragments longer than 300 bp were not included in the sequence alignment target, which partly contributes to the sharp drop in coverage at 300 bp in (c,d).

Techniques Used: In Silico, Produced, Sequencing

2) Product Images from "Combined Targeted DNA Sequencing in Non-Small Cell Lung Cancer (NSCLC) Using UNCseq and NGScopy, and RNA Sequencing Using UNCqeR for the Detection of Genetic Aberrations in NSCLC"

Article Title: Combined Targeted DNA Sequencing in Non-Small Cell Lung Cancer (NSCLC) Using UNCseq and NGScopy, and RNA Sequencing Using UNCqeR for the Detection of Genetic Aberrations in NSCLC

Journal: PLoS ONE

doi: 10.1371/journal.pone.0129280

Depth and Breadth of On-Target Coverage of the 100 Lung Cancer Samples. Shown for each tumor specimen is the percentage of targeted bases covered at given coverage depth (1x, 20x, 50x, 100x) and sequenced under different lane settings in the HiSeq 2000 instrument (2, 4, and 8 DNA libraries per lane, Lib/Ln).
Figure Legend Snippet: Depth and Breadth of On-Target Coverage of the 100 Lung Cancer Samples. Shown for each tumor specimen is the percentage of targeted bases covered at given coverage depth (1x, 20x, 50x, 100x) and sequenced under different lane settings in the HiSeq 2000 instrument (2, 4, and 8 DNA libraries per lane, Lib/Ln).

Techniques Used:

3) Product Images from "Technical Considerations for Reduced Representation Bisulfite Sequencing with Multiplexed Libraries"

Article Title: Technical Considerations for Reduced Representation Bisulfite Sequencing with Multiplexed Libraries

Journal: Journal of Biomedicine and Biotechnology

doi: 10.1155/2012/741542

Comparison of base-calling between regular genomic libraries and RRBS libraries. Relative intensities for each base channel are shown across the 200 cycles of a 100 bp paired-end HiSeq 2000 sequencing run. A. Regular multiplexed human genomic libraries, lane 4. B. Multiplexed RRBS libraries, lane 8.
Figure Legend Snippet: Comparison of base-calling between regular genomic libraries and RRBS libraries. Relative intensities for each base channel are shown across the 200 cycles of a 100 bp paired-end HiSeq 2000 sequencing run. A. Regular multiplexed human genomic libraries, lane 4. B. Multiplexed RRBS libraries, lane 8.

Techniques Used: Sequencing

4) Product Images from "Effective Optimization of Antibody Affinity by Phage Display Integrated with High-Throughput DNA Synthesis and Sequencing Technologies"

Article Title: Effective Optimization of Antibody Affinity by Phage Display Integrated with High-Throughput DNA Synthesis and Sequencing Technologies

Journal: PLoS ONE

doi: 10.1371/journal.pone.0129125

Overall scheme of the HuA21 affinity maturation process by phage display. (A) Candidate residues from the L1, L3, H1, H2 and H3 CDRs were targeted for diversification. (B) NWG, NWC and NSG degenerate codons were used for amino acid saturation mutagenesis. (C) Degenerate oligonucleotides containing CDR mutations were synthesized on microchips. (D) Mutant CDR libraries were amplified from the mixture of released oligonucleotides. (E) Mutant scFv gene libraries were assembled by OE-PCR and transformed into TG1. (F) Recombinant phages were amplified with helper phages, captured on antigen-coated magnetic beads, and recovered by re-infecting TG1. (G) Phage ELISA was used to isolate antigen-binding clones. (H) Positive clones were submitted for Sanger sequencing. (I) Pool of mutant CDR libraries was sequenced on the Illumina HiSeq 2000 platform. (J) NGS data was analyzed after quality filtration. (K) ScFv-Fc mutants were expressed in Expi293 cells to determine binding EC50s.
Figure Legend Snippet: Overall scheme of the HuA21 affinity maturation process by phage display. (A) Candidate residues from the L1, L3, H1, H2 and H3 CDRs were targeted for diversification. (B) NWG, NWC and NSG degenerate codons were used for amino acid saturation mutagenesis. (C) Degenerate oligonucleotides containing CDR mutations were synthesized on microchips. (D) Mutant CDR libraries were amplified from the mixture of released oligonucleotides. (E) Mutant scFv gene libraries were assembled by OE-PCR and transformed into TG1. (F) Recombinant phages were amplified with helper phages, captured on antigen-coated magnetic beads, and recovered by re-infecting TG1. (G) Phage ELISA was used to isolate antigen-binding clones. (H) Positive clones were submitted for Sanger sequencing. (I) Pool of mutant CDR libraries was sequenced on the Illumina HiSeq 2000 platform. (J) NGS data was analyzed after quality filtration. (K) ScFv-Fc mutants were expressed in Expi293 cells to determine binding EC50s.

Techniques Used: Mutagenesis, Synthesized, Amplification, Overlap Extension Polymerase Chain Reaction, Transformation Assay, Recombinant, Magnetic Beads, Enzyme-linked Immunosorbent Assay, Binding Assay, Clone Assay, Sequencing, Next-Generation Sequencing, Filtration

5) Product Images from "Assessment of microRNA differential expression and detection in multiplexed small RNA sequencing data"

Article Title: Assessment of microRNA differential expression and detection in multiplexed small RNA sequencing data

Journal: RNA

doi: 10.1261/rna.046060.114

Sequencing of multiplexed small RNA samples. ( A ) Using the Illumina TruSeq kit, lung tissue samples were given a unique index, placed in pools consisting of one, three, six, nine, or 12 samples, and sequenced using the Illumina HiSeq 2000. The same six
Figure Legend Snippet: Sequencing of multiplexed small RNA samples. ( A ) Using the Illumina TruSeq kit, lung tissue samples were given a unique index, placed in pools consisting of one, three, six, nine, or 12 samples, and sequenced using the Illumina HiSeq 2000. The same six

Techniques Used: Sequencing

Related Articles

Flow Cytometry:

Article Title: Technical Considerations for Reduced Representation Bisulfite Sequencing with Multiplexed Libraries
Article Snippet: .. Sequencing Libraries were pooled at equimolar concentrations based on Qubit measurements and sequenced on a single flow cell lane of an Illumina HiSeq 2000 sequencer with a paired end, 100 bp run. ..

Article Title: Integrated Omic Analyses Provide Evidence that a “Candidatus Accumulibacter phosphatis” Strain Performs Denitrification under Microaerobic Conditions
Article Snippet: .. Sequencing of the flow cell was performed on an Illumina HiSeq 2000 sequencer using a TruSeq SBS sequencing kit (v3; 200 cycles), following a 2 × 150 indexed run recipe. .. Sequence data were deposited at IMG/M under Taxon Object identifiers (IDs) 3300004259, 3300004260, and 3300004621 to 3300004624 ( https://genome.jgi.doe.gov/portal/ ).

Amplification:

Article Title: High-density SNP linkage map construction and QTL mapping for flavonoid-related traits in a tea plant (Camellia sinensis) using 2b-RAD sequencing
Article Snippet: .. In the second PCR amplification, a specific adapter barcode was incorporated into each library; then, all libraries were pooled for single-end sequencing (1 × 50 bp) using an Illumina HiSeq 2000 sequencer. ..

RNA Sequencing Assay:

Article Title: Assessment of microRNA differential expression and detection in multiplexed small RNA sequencing data
Article Snippet: .. Multiplexed small RNA sequencing was conducted on the Illumina HiSeq 2000 sequencer according to the manufacturer's protocol. .. Briefly, 1 µg of total RNA from each sample was used for library preparation with the TruSeq Small RNA Sample Prep Kit (Illumina).

Produced:

Article Title: Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling
Article Snippet: .. To evaluate the performance of the mRRBS protocol, we sequenced the 96 libraries using 8 lanes of an Illumina HiSeq 2000 sequencer with 12 libraries per lane, which produced a median of 11.3 million reads per library (Table and Figure ; Additional file ). ..

Sequencing:

Article Title: Technical Considerations for Reduced Representation Bisulfite Sequencing with Multiplexed Libraries
Article Snippet: .. Sequencing Libraries were pooled at equimolar concentrations based on Qubit measurements and sequenced on a single flow cell lane of an Illumina HiSeq 2000 sequencer with a paired end, 100 bp run. ..

Article Title: Integrated Omic Analyses Provide Evidence that a “Candidatus Accumulibacter phosphatis” Strain Performs Denitrification under Microaerobic Conditions
Article Snippet: .. Sequencing of the flow cell was performed on an Illumina HiSeq 2000 sequencer using a TruSeq SBS sequencing kit (v3; 200 cycles), following a 2 × 150 indexed run recipe. .. Sequence data were deposited at IMG/M under Taxon Object identifiers (IDs) 3300004259, 3300004260, and 3300004621 to 3300004624 ( https://genome.jgi.doe.gov/portal/ ).

Article Title: High-density SNP linkage map construction and QTL mapping for flavonoid-related traits in a tea plant (Camellia sinensis) using 2b-RAD sequencing
Article Snippet: .. In the second PCR amplification, a specific adapter barcode was incorporated into each library; then, all libraries were pooled for single-end sequencing (1 × 50 bp) using an Illumina HiSeq 2000 sequencer. ..

Generated:

Article Title: Combined Targeted DNA Sequencing in Non-Small Cell Lung Cancer (NSCLC) Using UNCseq and NGScopy, and RNA Sequencing Using UNCqeR for the Detection of Genetic Aberrations in NSCLC
Article Snippet: .. Clusters were then generated using TruSeq SR Cluster Kit v.2 and were loaded into the HiSeq 2000 sequencer (Illumina Inc., San Diego, CA). .. Sequencing by synthesis [ ] was performed using standard single-indexed libraries on either single-read (07–0120) or paired-end (11–1115) flow cells with 100 cycles (ClinSeq 1 x 100-bp or 2 x 100-bp, respectively) and an index read (‘barcode’) consisting of 7 cycles of sequencing using the Illumina TruSeq SBS v.3 chemistry. summarizes key differences in sample processing and sequencing between the 07–0120 and 11–1115 tumor tissue cohorts.

Polymerase Chain Reaction:

Article Title: High-density SNP linkage map construction and QTL mapping for flavonoid-related traits in a tea plant (Camellia sinensis) using 2b-RAD sequencing
Article Snippet: .. In the second PCR amplification, a specific adapter barcode was incorporated into each library; then, all libraries were pooled for single-end sequencing (1 × 50 bp) using an Illumina HiSeq 2000 sequencer. ..

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    Illumina Inc illumina hiseq 2000
    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using <t>Illumina</t> <t>Hiseq</t> 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.
    Illumina Hiseq 2000, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 5538 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina hiseq 2000/product/Illumina Inc
    Average 99 stars, based on 5538 article reviews
    Price from $9.99 to $1999.99
    illumina hiseq 2000 - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    86
    Illumina Inc hiseq 2000 sequencing runs
    Analysis of the relative abundance, GC content and likelihood of secondary structure formation for each of the 100 expected Illumina-compatible barcode sequences. ( A ) Relative abundance of the 100 expected barcode sequences, as detected during the first and second sequencing runs using the Illumina <t>HiSeq</t> 2000 (Pearson r (98) = 0.93, p
    Hiseq 2000 Sequencing Runs, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 sequencing runs/product/Illumina Inc
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 sequencing runs - by Bioz Stars, 2020-05
    86/100 stars
      Buy from Supplier

    92
    Illumina Inc hiseq 2000 sequencer
    Performance summary of mRRBS . Ninety-six samples were processed using mRRBS and sequenced with eight lanes of Illumina <t>HiSeq</t> 2000 using 12 barcoded adapters per lane. (a) The total number of reads for each sample is shown 84 samples with > 5 million total reads were included in the subsequent comparisons. (b) Quartile plots of summary coverage depth from these samples. The minimum and maximum values are bounded by the light blue area in (b-d), while the darker blue area represents the interquartile range. The dark blue line indicates the median. (c,d) MspI in silico digestion of the hg19 genome produced a total of 1,124,739 fragments. (c) The percentage of fragments of each fragment size that were covered by at least one read. (d) The average coverage depth for fragments of each length. Genomic MspI-digested fragments longer than 300 bp were not included in the sequence alignment target, which partly contributes to the sharp drop in coverage at 300 bp in (c,d).
    Hiseq 2000 Sequencer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 1047 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiseq 2000 sequencer/product/Illumina Inc
    Average 92 stars, based on 1047 article reviews
    Price from $9.99 to $1999.99
    hiseq 2000 sequencer - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using Illumina Hiseq 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.

    Journal: PLoS ONE

    Article Title: Systematic Analysis of Small RNAs Associated with Human Mitochondria by Deep Sequencing: Detailed Analysis of Mitochondrial Associated miRNA

    doi: 10.1371/journal.pone.0044873

    Figure Lengend Snippet: Generation and analysis of sRNA sequences from mitochondria. sRNA library generated from mitochondria from HEK293 and HeLa were sequenced using Illumina Hiseq 2000 platform that generated 19089819 and 17312962 clean sequence respectively. (A) Venn diagram showing distribution of common and specific sRNA total sequence reads amongst the two libraries. (B) Venn diagram showing distribution of common and specific sRNA unique sequence reads amongst the two libraries. (C) Length distribution and frequency percent of sequences in HEK293 and HeLa mitochondrial sRNA libraries.

    Article Snippet: Bioinformatics Analysis of sRNA Libraries from Mitochondria All the 50 nt sequence tags generated from Illumina HiSeq 2000 went through data cleaning process which included getting rid of low quality tags.

    Techniques: Generated, Sequencing

    The growth of SRA data categorized by project types, and sequencing platforms. (A) The growth of the number of SRA studies categorized by project types. The number of studies are double that of the previous year. (B) The growth of the number of SRA experiments categorized by sequencing platforms. Over 200,000 experiments are submitted under approximately 14,000 studies. The experiments using Illumina HiSeq 2000 are dramatically increasing.

    Journal: PLoS ONE

    Article Title: Experimental Design-Based Functional Mining and Characterization of High-Throughput Sequencing Data in the Sequence Read Archive

    doi: 10.1371/journal.pone.0077910

    Figure Lengend Snippet: The growth of SRA data categorized by project types, and sequencing platforms. (A) The growth of the number of SRA studies categorized by project types. The number of studies are double that of the previous year. (B) The growth of the number of SRA experiments categorized by sequencing platforms. Over 200,000 experiments are submitted under approximately 14,000 studies. The experiments using Illumina HiSeq 2000 are dramatically increasing.

    Article Snippet: The top 3 of most used sequencer are Illumina HiSeq 2000, Illumina Genome Analyzer II, and 454 GS FLX Titanium (92,888, 42,274, and 19,463 experiments, respectively), and experiments with new sequencing platforms such as Complete Genomics, Helicos HeliScope, PacBio RS, and Ion Torrent PGM are also archived (1133, 437, 431, and 304 experiments, respectively).

    Techniques: Sequencing

    Analysis of the relative abundance, GC content and likelihood of secondary structure formation for each of the 100 expected Illumina-compatible barcode sequences. ( A ) Relative abundance of the 100 expected barcode sequences, as detected during the first and second sequencing runs using the Illumina HiSeq 2000 (Pearson r (98) = 0.93, p

    Journal: Nucleic Acids Research

    Article Title: Impact of next-generation sequencing error on analysis of barcoded plasmid libraries of known complexity and sequence

    doi: 10.1093/nar/gku607

    Figure Lengend Snippet: Analysis of the relative abundance, GC content and likelihood of secondary structure formation for each of the 100 expected Illumina-compatible barcode sequences. ( A ) Relative abundance of the 100 expected barcode sequences, as detected during the first and second sequencing runs using the Illumina HiSeq 2000 (Pearson r (98) = 0.93, p

    Article Snippet: The samples analyzed included the two post-amplification samples that were used in the Illumina HiSeq 2000 sequencing runs, of which insufficient quantities remained for analysis using semi-quantitative PCR.

    Techniques: Sequencing

    Analysis of the position and substitution-like type of error for all one-mismatch sequence errors for both Illumina HiSeq 2000 sequencing runs. One-mismatch errors were compared to the known barcode sequences from which they were derived. Errors from the first sequencing run represent the sum of one-mismatch errors after Q30 quality filtering for the one-barcode sample and 10- and 100-barcode libraries, although one-mismatch errors from the 100-barcode library comprise 95.3% of all errors. Errors from the second sequencing run represent one-mismatch errors after Q30 quality filtering for the 100-barcode library. ( A ) Distribution of one-mismatch errors across each position of the barcode (positions 2–16 of the sequence reads). This distribution differed significantly from an expected even distribution (χ 2 = 30 064, df = 14, p

    Journal: Nucleic Acids Research

    Article Title: Impact of next-generation sequencing error on analysis of barcoded plasmid libraries of known complexity and sequence

    doi: 10.1093/nar/gku607

    Figure Lengend Snippet: Analysis of the position and substitution-like type of error for all one-mismatch sequence errors for both Illumina HiSeq 2000 sequencing runs. One-mismatch errors were compared to the known barcode sequences from which they were derived. Errors from the first sequencing run represent the sum of one-mismatch errors after Q30 quality filtering for the one-barcode sample and 10- and 100-barcode libraries, although one-mismatch errors from the 100-barcode library comprise 95.3% of all errors. Errors from the second sequencing run represent one-mismatch errors after Q30 quality filtering for the 100-barcode library. ( A ) Distribution of one-mismatch errors across each position of the barcode (positions 2–16 of the sequence reads). This distribution differed significantly from an expected even distribution (χ 2 = 30 064, df = 14, p

    Article Snippet: The samples analyzed included the two post-amplification samples that were used in the Illumina HiSeq 2000 sequencing runs, of which insufficient quantities remained for analysis using semi-quantitative PCR.

    Techniques: Sequencing, Derivative Assay

    Performance summary of mRRBS . Ninety-six samples were processed using mRRBS and sequenced with eight lanes of Illumina HiSeq 2000 using 12 barcoded adapters per lane. (a) The total number of reads for each sample is shown 84 samples with > 5 million total reads were included in the subsequent comparisons. (b) Quartile plots of summary coverage depth from these samples. The minimum and maximum values are bounded by the light blue area in (b-d), while the darker blue area represents the interquartile range. The dark blue line indicates the median. (c,d) MspI in silico digestion of the hg19 genome produced a total of 1,124,739 fragments. (c) The percentage of fragments of each fragment size that were covered by at least one read. (d) The average coverage depth for fragments of each length. Genomic MspI-digested fragments longer than 300 bp were not included in the sequence alignment target, which partly contributes to the sharp drop in coverage at 300 bp in (c,d).

    Journal: Genome Biology

    Article Title: Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling

    doi: 10.1186/gb-2012-13-10-r92

    Figure Lengend Snippet: Performance summary of mRRBS . Ninety-six samples were processed using mRRBS and sequenced with eight lanes of Illumina HiSeq 2000 using 12 barcoded adapters per lane. (a) The total number of reads for each sample is shown 84 samples with > 5 million total reads were included in the subsequent comparisons. (b) Quartile plots of summary coverage depth from these samples. The minimum and maximum values are bounded by the light blue area in (b-d), while the darker blue area represents the interquartile range. The dark blue line indicates the median. (c,d) MspI in silico digestion of the hg19 genome produced a total of 1,124,739 fragments. (c) The percentage of fragments of each fragment size that were covered by at least one read. (d) The average coverage depth for fragments of each length. Genomic MspI-digested fragments longer than 300 bp were not included in the sequence alignment target, which partly contributes to the sharp drop in coverage at 300 bp in (c,d).

    Article Snippet: To evaluate the performance of the mRRBS protocol, we sequenced the 96 libraries using 8 lanes of an Illumina HiSeq 2000 sequencer with 12 libraries per lane, which produced a median of 11.3 million reads per library (Table and Figure ; Additional file ).

    Techniques: In Silico, Produced, Sequencing