hiscribe t7 high yield rna synthesis kit  (New England Biolabs)


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    New England Biolabs hiscribe t7 high yield rna synthesis kit
    Hiscribe T7 High Yield Rna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiscribe t7 high yield rna synthesis kit/product/New England Biolabs
    Average 99 stars, based on 179 article reviews
    Price from $9.99 to $1999.99
    hiscribe t7 high yield rna synthesis kit - by Bioz Stars, 2020-05
    99/100 stars

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    Amplification:

    Article Title: G-quadruplex structures trigger RNA phase separation
    Article Snippet: .. Preparation of in vitro transcribed RNAs DNA Templates (see ) were amplified and purified, RNAs were in vitro transcribed using Hiscribe T7 High Yield RNA Synthesis Kit (New England Biolabs, NEB). .. Each RNA was purified using pre-casted 6% denaturing polyacrylamide gel (Thermo Fisher Scientific) and the gel band with desired RNA product was cut and soaked in 1× TEL800 buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA, 800 mM LiCl) overnight, RNA was then purified with ZYMO RNA purification kit (ZYMO S1016).

    Agarose Gel Electrophoresis:

    Article Title: Delivery of Cas9/sgRNA Ribonucleoprotein Complexes via Hydroxystearyl Oligoamino Amides.
    Article Snippet: .. The linear DNA fragments containing the T7 promoter followed by the sgRNA sequence were analyzed on an agarose gel and transcribed in vitro using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Germany) according to the manufacturer’s instructions. .. ATTO488-labeled sgRNA was synthesized by substitution of 7% of the UTPs with aminoallyl-UTPATTO488 (Jena Bioscience, Germany) during in vitro transcription.

    In Vitro:

    Article Title: Good guide, bad guide: spacer sequence-dependent cleavage efficiency of Cas12a
    Article Snippet: .. In vitro cleavage assay Pre-crRNA was made by in vitro transcription with the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). .. FnCas12a was diluted to 0.4 nM in 2× NEBuffer 4 with 2 mM Mg2+ , instead of 20 mM Mg2+ .

    Article Title: G-quadruplex structures trigger RNA phase separation
    Article Snippet: .. Preparation of in vitro transcribed RNAs DNA Templates (see ) were amplified and purified, RNAs were in vitro transcribed using Hiscribe T7 High Yield RNA Synthesis Kit (New England Biolabs, NEB). .. Each RNA was purified using pre-casted 6% denaturing polyacrylamide gel (Thermo Fisher Scientific) and the gel band with desired RNA product was cut and soaked in 1× TEL800 buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA, 800 mM LiCl) overnight, RNA was then purified with ZYMO RNA purification kit (ZYMO S1016).

    Article Title: Massively multiplexed nucleic acid detection using Cas13.
    Article Snippet: .. SNP detection crRNAs were transcribed from annealed DNA templates in vitro using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). ..

    Article Title: Profiling the transcriptome by RNA SPOTs
    Article Snippet: .. The PCR products were used as the template for in vitro transcription (E2040S; NEB) followed by reverse transcription (EP7051; Thermo Fischer) with the forward primer. .. After alkaline hydrolysis, the single stranded DNA (ssDNA) probes were purified by ethanol precipitation and resuspend in primary probe hybridization buffer comprising of 30% formamide (F9037; Sigma), 2× SSC (15557036; Thermo Fischer), and 10% (w/v) Dextran Sulfate (D8906; Sigma).

    Article Title: An RNA Hybridization Assay for Screening Influenza A Virus Polymerase Inhibitors Using the Entire Ribonucleoprotein Complex.
    Article Snippet: .. Novel antiviral drugs, which are less prone to resistance development, are desirable alternatives to the currently approved drugs for the treatment of potentially serious influenza virus infections. ..

    Article Title: Delivery of Cas9/sgRNA Ribonucleoprotein Complexes via Hydroxystearyl Oligoamino Amides.
    Article Snippet: .. The linear DNA fragments containing the T7 promoter followed by the sgRNA sequence were analyzed on an agarose gel and transcribed in vitro using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Germany) according to the manufacturer’s instructions. .. ATTO488-labeled sgRNA was synthesized by substitution of 7% of the UTPs with aminoallyl-UTPATTO488 (Jena Bioscience, Germany) during in vitro transcription.

    Synthesized:

    Article Title: Expression Profiling, Downstream Signaling, and Inter-subunit Interactions of GPA2/GPB5 in the Adult Mosquito Aedes aegypti
    Article Snippet: .. Digoxigenin (DIG)-labeled anti-sense and sense RNA probes corresponding to GPA2 and GPB5 subunits were synthesized using the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, Whitby, ON, Canada). .. Fluorescence in situ hybridization (FISH) was then used to detect GPA2 and/or GPB5 transcript in the mosquito central nervous system using 4 ng μl−1 (GPB5) and/or 6 ng μl−1 (GPA2) RNA sense/antisense probes, following a previously established protocol ( ).

    Article Title: An RNA Hybridization Assay for Screening Influenza A Virus Polymerase Inhibitors Using the Entire Ribonucleoprotein Complex.
    Article Snippet: .. Novel antiviral drugs, which are less prone to resistance development, are desirable alternatives to the currently approved drugs for the treatment of potentially serious influenza virus infections. ..

    Purification:

    Article Title: G-quadruplex structures trigger RNA phase separation
    Article Snippet: .. Preparation of in vitro transcribed RNAs DNA Templates (see ) were amplified and purified, RNAs were in vitro transcribed using Hiscribe T7 High Yield RNA Synthesis Kit (New England Biolabs, NEB). .. Each RNA was purified using pre-casted 6% denaturing polyacrylamide gel (Thermo Fisher Scientific) and the gel band with desired RNA product was cut and soaked in 1× TEL800 buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA, 800 mM LiCl) overnight, RNA was then purified with ZYMO RNA purification kit (ZYMO S1016).

    Article Title: An RNA Hybridization Assay for Screening Influenza A Virus Polymerase Inhibitors Using the Entire Ribonucleoprotein Complex.
    Article Snippet: .. Novel antiviral drugs, which are less prone to resistance development, are desirable alternatives to the currently approved drugs for the treatment of potentially serious influenza virus infections. ..

    Polymerase Chain Reaction:

    Article Title: Profiling the transcriptome by RNA SPOTs
    Article Snippet: .. The PCR products were used as the template for in vitro transcription (E2040S; NEB) followed by reverse transcription (EP7051; Thermo Fischer) with the forward primer. .. After alkaline hydrolysis, the single stranded DNA (ssDNA) probes were purified by ethanol precipitation and resuspend in primary probe hybridization buffer comprising of 30% formamide (F9037; Sigma), 2× SSC (15557036; Thermo Fischer), and 10% (w/v) Dextran Sulfate (D8906; Sigma).

    Incubation:

    Article Title: An RNA Hybridization Assay for Screening Influenza A Virus Polymerase Inhibitors Using the Entire Ribonucleoprotein Complex.
    Article Snippet: .. Novel antiviral drugs, which are less prone to resistance development, are desirable alternatives to the currently approved drugs for the treatment of potentially serious influenza virus infections. ..

    Sequencing:

    Article Title: Delivery of Cas9/sgRNA Ribonucleoprotein Complexes via Hydroxystearyl Oligoamino Amides.
    Article Snippet: .. The linear DNA fragments containing the T7 promoter followed by the sgRNA sequence were analyzed on an agarose gel and transcribed in vitro using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Germany) according to the manufacturer’s instructions. .. ATTO488-labeled sgRNA was synthesized by substitution of 7% of the UTPs with aminoallyl-UTPATTO488 (Jena Bioscience, Germany) during in vitro transcription.

    Derivative Assay:

    Article Title: An RNA Hybridization Assay for Screening Influenza A Virus Polymerase Inhibitors Using the Entire Ribonucleoprotein Complex.
    Article Snippet: .. Novel antiviral drugs, which are less prone to resistance development, are desirable alternatives to the currently approved drugs for the treatment of potentially serious influenza virus infections. ..

    Cleavage Assay:

    Article Title: Good guide, bad guide: spacer sequence-dependent cleavage efficiency of Cas12a
    Article Snippet: .. In vitro cleavage assay Pre-crRNA was made by in vitro transcription with the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). .. FnCas12a was diluted to 0.4 nM in 2× NEBuffer 4 with 2 mM Mg2+ , instead of 20 mM Mg2+ .

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    New England Biolabs t7 quick high yield rna synthesis kit
    The effect of long ssRNA on dsDNA extension at low force (e.g. Figure 2B ) is dependent on concentration, homology, and length. Box-and-whisker plots (see Materials and Methods) show the slopes of the low force–extension curves (Figure 2B ) of the indicated samples. ( A ) <t>DNA:RNA</t> molar ratio was varied from 1:1 to 1:10 5 . Statistical analysis (asterisks) compared 1:1 versus all other ratios. ( B ) The effects of non-homologous ssRNA controls. Fluc is a 1766-nt control transcript provided in the <t>T7</t> Quick High Yield RNA Synthesis Kit, showing 43% identity with the 37 441–39 524 region of the genome of λ dsDNA. Y-RNA is the total RNA of S. cerevisiae strain. For 6KM13, bacteriophage M13mp18 circular ssDNA ∼7200 nt was amplified as the template for in vitro transcription. The resulting ssRNA is 6001 nt and presents 44% identity with the genome of λ dsDNA (region 22 893–31 255). 6L and 6H are ∼6k nt species homologous to adjacent regions of λ dsDNA (Figure 1B ). DNA:RNA molar ratio = 1:1000 in all samples; statistical analysis was performed for the ratio of λ dsDNA alone versus all other ratios. ( C ) The effects of various lengths of ssRNA (described in Figure 1B ), DNA:RNA molar ratio = 1:1000. (a, b, c) n = 10, * P
    T7 Quick High Yield Rna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t7 quick high yield rna synthesis kit/product/New England Biolabs
    Average 99 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    t7 quick high yield rna synthesis kit - by Bioz Stars, 2020-05
    99/100 stars
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    The effect of long ssRNA on dsDNA extension at low force (e.g. Figure 2B ) is dependent on concentration, homology, and length. Box-and-whisker plots (see Materials and Methods) show the slopes of the low force–extension curves (Figure 2B ) of the indicated samples. ( A ) DNA:RNA molar ratio was varied from 1:1 to 1:10 5 . Statistical analysis (asterisks) compared 1:1 versus all other ratios. ( B ) The effects of non-homologous ssRNA controls. Fluc is a 1766-nt control transcript provided in the T7 Quick High Yield RNA Synthesis Kit, showing 43% identity with the 37 441–39 524 region of the genome of λ dsDNA. Y-RNA is the total RNA of S. cerevisiae strain. For 6KM13, bacteriophage M13mp18 circular ssDNA ∼7200 nt was amplified as the template for in vitro transcription. The resulting ssRNA is 6001 nt and presents 44% identity with the genome of λ dsDNA (region 22 893–31 255). 6L and 6H are ∼6k nt species homologous to adjacent regions of λ dsDNA (Figure 1B ). DNA:RNA molar ratio = 1:1000 in all samples; statistical analysis was performed for the ratio of λ dsDNA alone versus all other ratios. ( C ) The effects of various lengths of ssRNA (described in Figure 1B ), DNA:RNA molar ratio = 1:1000. (a, b, c) n = 10, * P

    Journal: Nucleic Acids Research

    Article Title: Single molecule identification of homology-dependent interactions between long ssRNA and dsDNA

    doi: 10.1093/nar/gkw758

    Figure Lengend Snippet: The effect of long ssRNA on dsDNA extension at low force (e.g. Figure 2B ) is dependent on concentration, homology, and length. Box-and-whisker plots (see Materials and Methods) show the slopes of the low force–extension curves (Figure 2B ) of the indicated samples. ( A ) DNA:RNA molar ratio was varied from 1:1 to 1:10 5 . Statistical analysis (asterisks) compared 1:1 versus all other ratios. ( B ) The effects of non-homologous ssRNA controls. Fluc is a 1766-nt control transcript provided in the T7 Quick High Yield RNA Synthesis Kit, showing 43% identity with the 37 441–39 524 region of the genome of λ dsDNA. Y-RNA is the total RNA of S. cerevisiae strain. For 6KM13, bacteriophage M13mp18 circular ssDNA ∼7200 nt was amplified as the template for in vitro transcription. The resulting ssRNA is 6001 nt and presents 44% identity with the genome of λ dsDNA (region 22 893–31 255). 6L and 6H are ∼6k nt species homologous to adjacent regions of λ dsDNA (Figure 1B ). DNA:RNA molar ratio = 1:1000 in all samples; statistical analysis was performed for the ratio of λ dsDNA alone versus all other ratios. ( C ) The effects of various lengths of ssRNA (described in Figure 1B ), DNA:RNA molar ratio = 1:1000. (a, b, c) n = 10, * P

    Article Snippet: Fluc is a 1766-nt control transcript provided in the T7 Quick High Yield RNA Synthesis Kit, showing 43% identity with the 37 441–39 524 region of the genome of λ dsDNA.

    Techniques: Concentration Assay, Whisker Assay, Amplification, In Vitro