his tagged cour protease inhibitor cocktail  (Thermo Fisher)


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    Structured Review

    Thermo Fisher his tagged cour protease inhibitor cocktail
    Identification of the <t>CouR</t> binding site in the <t>couA</t> promoter region by DNase I footprinting. A 439-bp, 6-FAM-labeled DNA fragment (0.45 pmol) was incubated in the presence or absence of CouR (0, 3, and 6 pmol) and p -coumaroyl-CoA (pC-CoA) (2.2 nmol) and
    His Tagged Cour Protease Inhibitor Cocktail, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/his tagged cour protease inhibitor cocktail/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    his tagged cour protease inhibitor cocktail - by Bioz Stars, 2020-05
    85/100 stars

    Related Products / Commonly Used Together

    his-tagged coua
    his-tagged coub
    nacl
    tris

    Images

    1) Product Images from "Anaerobic p-Coumarate Degradation by Rhodopseudomonas palustris and Identification of CouR, a MarR Repressor Protein That Binds p-Coumaroyl Coenzyme A"

    Article Title: Anaerobic p-Coumarate Degradation by Rhodopseudomonas palustris and Identification of CouR, a MarR Repressor Protein That Binds p-Coumaroyl Coenzyme A

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.06817-11

    Identification of the CouR binding site in the couA promoter region by DNase I footprinting. A 439-bp, 6-FAM-labeled DNA fragment (0.45 pmol) was incubated in the presence or absence of CouR (0, 3, and 6 pmol) and p -coumaroyl-CoA (pC-CoA) (2.2 nmol) and
    Figure Legend Snippet: Identification of the CouR binding site in the couA promoter region by DNase I footprinting. A 439-bp, 6-FAM-labeled DNA fragment (0.45 pmol) was incubated in the presence or absence of CouR (0, 3, and 6 pmol) and p -coumaroyl-CoA (pC-CoA) (2.2 nmol) and

    Techniques Used: Binding Assay, Footprinting, Labeling, Incubation

    Gel shift assay showing binding of CouR to the couA promoter (A) and the effect of p -coumaroyl-CoA on CouR binding activity (B). (A) CouR protein (0, 2, 4, or 8 pmol) was added to reaction mixtures containing 0.3 pmol of DNA probe. DNA upstream of rpa3929
    Figure Legend Snippet: Gel shift assay showing binding of CouR to the couA promoter (A) and the effect of p -coumaroyl-CoA on CouR binding activity (B). (A) CouR protein (0, 2, 4, or 8 pmol) was added to reaction mixtures containing 0.3 pmol of DNA probe. DNA upstream of rpa3929

    Techniques Used: Electrophoretic Mobility Shift Assay, Binding Assay, Activity Assay

    The couA promoter sequence and transcriptional start site in the couA upstream region. (A) S1 nuclease analysis of the couA transcript from the wild type and couR mutant. R. palustris cells were grown in PM plus succinate. The couA transcript was detected
    Figure Legend Snippet: The couA promoter sequence and transcriptional start site in the couA upstream region. (A) S1 nuclease analysis of the couA transcript from the wild type and couR mutant. R. palustris cells were grown in PM plus succinate. The couA transcript was detected

    Techniques Used: Sequencing, Mutagenesis

    Related Articles

    Protease Inhibitor:

    Article Title: Anaerobic p-Coumarate Degradation by Rhodopseudomonas palustris and Identification of CouR, a MarR Repressor Protein That Binds p-Coumaroyl Coenzyme A
    Article Snippet: .. The cell pellets were suspended in 20 mM Tris (pH 7.9), 500 mM NaCl, 10% glycerol with (for His-tagged CouB) or without (for His-tagged CouA and His-tagged CouR) protease inhibitor cocktail (Thermo Scientific, Santa Clara, CA) and lysed by sonication. .. The lysate was centrifuged, and the resulting supernatant was mixed with nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, Valencia, CA) for 1 h. For His10 -CouB, the agarose was washed with 10 mM imidazole and then His10 -CouB was eluted with 50 to 200 mM imidazole.

    Sonication:

    Article Title: Anaerobic p-Coumarate Degradation by Rhodopseudomonas palustris and Identification of CouR, a MarR Repressor Protein That Binds p-Coumaroyl Coenzyme A
    Article Snippet: .. The cell pellets were suspended in 20 mM Tris (pH 7.9), 500 mM NaCl, 10% glycerol with (for His-tagged CouB) or without (for His-tagged CouA and His-tagged CouR) protease inhibitor cocktail (Thermo Scientific, Santa Clara, CA) and lysed by sonication. .. The lysate was centrifuged, and the resulting supernatant was mixed with nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen, Valencia, CA) for 1 h. For His10 -CouB, the agarose was washed with 10 mM imidazole and then His10 -CouB was eluted with 50 to 200 mM imidazole.

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    Thermo Fisher his tagged cour protease inhibitor cocktail
    Identification of the <t>CouR</t> binding site in the <t>couA</t> promoter region by DNase I footprinting. A 439-bp, 6-FAM-labeled DNA fragment (0.45 pmol) was incubated in the presence or absence of CouR (0, 3, and 6 pmol) and p -coumaroyl-CoA (pC-CoA) (2.2 nmol) and
    His Tagged Cour Protease Inhibitor Cocktail, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/his tagged cour protease inhibitor cocktail/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    his tagged cour protease inhibitor cocktail - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    Image Search Results


    Identification of the CouR binding site in the couA promoter region by DNase I footprinting. A 439-bp, 6-FAM-labeled DNA fragment (0.45 pmol) was incubated in the presence or absence of CouR (0, 3, and 6 pmol) and p -coumaroyl-CoA (pC-CoA) (2.2 nmol) and

    Journal: Journal of Bacteriology

    Article Title: Anaerobic p-Coumarate Degradation by Rhodopseudomonas palustris and Identification of CouR, a MarR Repressor Protein That Binds p-Coumaroyl Coenzyme A

    doi: 10.1128/JB.06817-11

    Figure Lengend Snippet: Identification of the CouR binding site in the couA promoter region by DNase I footprinting. A 439-bp, 6-FAM-labeled DNA fragment (0.45 pmol) was incubated in the presence or absence of CouR (0, 3, and 6 pmol) and p -coumaroyl-CoA (pC-CoA) (2.2 nmol) and

    Article Snippet: The cell pellets were suspended in 20 mM Tris (pH 7.9), 500 mM NaCl, 10% glycerol with (for His-tagged CouB) or without (for His-tagged CouA and His-tagged CouR) protease inhibitor cocktail (Thermo Scientific, Santa Clara, CA) and lysed by sonication.

    Techniques: Binding Assay, Footprinting, Labeling, Incubation

    Gel shift assay showing binding of CouR to the couA promoter (A) and the effect of p -coumaroyl-CoA on CouR binding activity (B). (A) CouR protein (0, 2, 4, or 8 pmol) was added to reaction mixtures containing 0.3 pmol of DNA probe. DNA upstream of rpa3929

    Journal: Journal of Bacteriology

    Article Title: Anaerobic p-Coumarate Degradation by Rhodopseudomonas palustris and Identification of CouR, a MarR Repressor Protein That Binds p-Coumaroyl Coenzyme A

    doi: 10.1128/JB.06817-11

    Figure Lengend Snippet: Gel shift assay showing binding of CouR to the couA promoter (A) and the effect of p -coumaroyl-CoA on CouR binding activity (B). (A) CouR protein (0, 2, 4, or 8 pmol) was added to reaction mixtures containing 0.3 pmol of DNA probe. DNA upstream of rpa3929

    Article Snippet: The cell pellets were suspended in 20 mM Tris (pH 7.9), 500 mM NaCl, 10% glycerol with (for His-tagged CouB) or without (for His-tagged CouA and His-tagged CouR) protease inhibitor cocktail (Thermo Scientific, Santa Clara, CA) and lysed by sonication.

    Techniques: Electrophoretic Mobility Shift Assay, Binding Assay, Activity Assay

    The couA promoter sequence and transcriptional start site in the couA upstream region. (A) S1 nuclease analysis of the couA transcript from the wild type and couR mutant. R. palustris cells were grown in PM plus succinate. The couA transcript was detected

    Journal: Journal of Bacteriology

    Article Title: Anaerobic p-Coumarate Degradation by Rhodopseudomonas palustris and Identification of CouR, a MarR Repressor Protein That Binds p-Coumaroyl Coenzyme A

    doi: 10.1128/JB.06817-11

    Figure Lengend Snippet: The couA promoter sequence and transcriptional start site in the couA upstream region. (A) S1 nuclease analysis of the couA transcript from the wild type and couR mutant. R. palustris cells were grown in PM plus succinate. The couA transcript was detected

    Article Snippet: The cell pellets were suspended in 20 mM Tris (pH 7.9), 500 mM NaCl, 10% glycerol with (for His-tagged CouB) or without (for His-tagged CouA and His-tagged CouR) protease inhibitor cocktail (Thermo Scientific, Santa Clara, CA) and lysed by sonication.

    Techniques: Sequencing, Mutagenesis