his mtdella1  (New England Biolabs)


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    Structured Review

    New England Biolabs his mtdella1
    Interactions between MtDELLAs and MtCYCLOPS/IPD3. ( a ) Yeast two-hybrid assays between IPD3 and <t>MtDELLA1,</t> MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pGADT7 (AD) and pGBKT7 (BD) vectors were plated onto SD/-Leu-Trp (-LT) medium or <t>SD/-Leu-Trp-Ade-His</t> (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound with HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP (N-terminal half of YFP) and IPD3-cYFP (C-terminal half of YFP). No protein interactions were detected in the other three combinations: MtDELLAs-nYFP--cYFP, IPD3-cYFP--nYFP and cYFP--nYFP. Scale bars, 20 μm.
    His Mtdella1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways"

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways

    Journal: Nature Communications

    doi: 10.1038/ncomms12433

    Interactions between MtDELLAs and MtCYCLOPS/IPD3. ( a ) Yeast two-hybrid assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pGADT7 (AD) and pGBKT7 (BD) vectors were plated onto SD/-Leu-Trp (-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound with HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP (N-terminal half of YFP) and IPD3-cYFP (C-terminal half of YFP). No protein interactions were detected in the other three combinations: MtDELLAs-nYFP--cYFP, IPD3-cYFP--nYFP and cYFP--nYFP. Scale bars, 20 μm.
    Figure Legend Snippet: Interactions between MtDELLAs and MtCYCLOPS/IPD3. ( a ) Yeast two-hybrid assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pGADT7 (AD) and pGBKT7 (BD) vectors were plated onto SD/-Leu-Trp (-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound with HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP (N-terminal half of YFP) and IPD3-cYFP (C-terminal half of YFP). No protein interactions were detected in the other three combinations: MtDELLAs-nYFP--cYFP, IPD3-cYFP--nYFP and cYFP--nYFP. Scale bars, 20 μm.

    Techniques Used: Construct, Expressing, Bimolecular Fluorescence Complementation Assay, Fluorescence, Transformation Assay

    Interactions between MtDELLAs and NSP2. ( a ) Yeast two-hybrid assays between NSP2 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pDEST-GADT7(AD) and pDEST-GBKT7(BD) vectors were plated onto SD/-Leu-Trp(-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP and NSP2-cYFP. There is no protein interaction in the other three combinations: MtDELLAs-nYFP--cYFP, NSP2-cYFP--nYFP and cYFP--nYFP ( Fig. 5c ). Scale bars, 20 μm.
    Figure Legend Snippet: Interactions between MtDELLAs and NSP2. ( a ) Yeast two-hybrid assays between NSP2 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pDEST-GADT7(AD) and pDEST-GBKT7(BD) vectors were plated onto SD/-Leu-Trp(-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP and NSP2-cYFP. There is no protein interaction in the other three combinations: MtDELLAs-nYFP--cYFP, NSP2-cYFP--nYFP and cYFP--nYFP ( Fig. 5c ). Scale bars, 20 μm.

    Techniques Used: Construct, Expressing, Bimolecular Fluorescence Complementation Assay, Fluorescence, Transformation Assay

    Related Articles

    Affinity Purification:

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways
    Article Snippet: Expression products were affinity purified via nickel-agarose (Qiagen) under the denaturing conditions by using 8 M urea. .. Purification of MBP-CCaMK, HIS-IPD3, HIS-MtDELLA1, HIS-MtDELLA2 and HIS-MtDELLA3 was performed according to the protocol of E8200 (New England Biolabs) and of BioSprint96 (Qiagen), respectively.

    Purification:

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways
    Article Snippet: Denatured proteins were refolded by stepwise dialysis . .. Purification of MBP-CCaMK, HIS-IPD3, HIS-MtDELLA1, HIS-MtDELLA2 and HIS-MtDELLA3 was performed according to the protocol of E8200 (New England Biolabs) and of BioSprint96 (Qiagen), respectively. .. IPD3 was phosphorylated in vitro by CCaMK as described .

    Chick Chorioallantoic Membrane Assay:

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways
    Article Snippet: Purification of MBP-CCaMK, HIS-IPD3, HIS-MtDELLA1, HIS-MtDELLA2 and HIS-MtDELLA3 was performed according to the protocol of E8200 (New England Biolabs) and of BioSprint96 (Qiagen), respectively. .. IPD3 was phosphorylated in vitro by CCaMK as described .

    Expressing:

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways
    Article Snippet: Expression products were affinity purified via nickel-agarose (Qiagen) under the denaturing conditions by using 8 M urea. .. Purification of MBP-CCaMK, HIS-IPD3, HIS-MtDELLA1, HIS-MtDELLA2 and HIS-MtDELLA3 was performed according to the protocol of E8200 (New England Biolabs) and of BioSprint96 (Qiagen), respectively.

    In Vitro:

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways
    Article Snippet: Paragraph title: In vitro phosphorylation ... Purification of MBP-CCaMK, HIS-IPD3, HIS-MtDELLA1, HIS-MtDELLA2 and HIS-MtDELLA3 was performed according to the protocol of E8200 (New England Biolabs) and of BioSprint96 (Qiagen), respectively.

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    New England Biolabs his mtdella1
    Interactions between MtDELLAs and MtCYCLOPS/IPD3. ( a ) Yeast two-hybrid assays between IPD3 and <t>MtDELLA1,</t> MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pGADT7 (AD) and pGBKT7 (BD) vectors were plated onto SD/-Leu-Trp (-LT) medium or <t>SD/-Leu-Trp-Ade-His</t> (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound with HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP (N-terminal half of YFP) and IPD3-cYFP (C-terminal half of YFP). No protein interactions were detected in the other three combinations: MtDELLAs-nYFP--cYFP, IPD3-cYFP--nYFP and cYFP--nYFP. Scale bars, 20 μm.
    His Mtdella1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/his mtdella1/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    his mtdella1 - by Bioz Stars, 2019-12
    79/100 stars
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    Image Search Results


    Interactions between MtDELLAs and MtCYCLOPS/IPD3. ( a ) Yeast two-hybrid assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pGADT7 (AD) and pGBKT7 (BD) vectors were plated onto SD/-Leu-Trp (-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound with HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP (N-terminal half of YFP) and IPD3-cYFP (C-terminal half of YFP). No protein interactions were detected in the other three combinations: MtDELLAs-nYFP--cYFP, IPD3-cYFP--nYFP and cYFP--nYFP. Scale bars, 20 μm.

    Journal: Nature Communications

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways

    doi: 10.1038/ncomms12433

    Figure Lengend Snippet: Interactions between MtDELLAs and MtCYCLOPS/IPD3. ( a ) Yeast two-hybrid assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pGADT7 (AD) and pGBKT7 (BD) vectors were plated onto SD/-Leu-Trp (-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound with HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP (N-terminal half of YFP) and IPD3-cYFP (C-terminal half of YFP). No protein interactions were detected in the other three combinations: MtDELLAs-nYFP--cYFP, IPD3-cYFP--nYFP and cYFP--nYFP. Scale bars, 20 μm.

    Article Snippet: Purification of MBP-CCaMK, HIS-IPD3, HIS-MtDELLA1, HIS-MtDELLA2 and HIS-MtDELLA3 was performed according to the protocol of E8200 (New England Biolabs) and of BioSprint96 (Qiagen), respectively.

    Techniques: Construct, Expressing, Bimolecular Fluorescence Complementation Assay, Fluorescence, Transformation Assay

    Interactions between MtDELLAs and NSP2. ( a ) Yeast two-hybrid assays between NSP2 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pDEST-GADT7(AD) and pDEST-GBKT7(BD) vectors were plated onto SD/-Leu-Trp(-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP and NSP2-cYFP. There is no protein interaction in the other three combinations: MtDELLAs-nYFP--cYFP, NSP2-cYFP--nYFP and cYFP--nYFP ( Fig. 5c ). Scale bars, 20 μm.

    Journal: Nature Communications

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways

    doi: 10.1038/ncomms12433

    Figure Lengend Snippet: Interactions between MtDELLAs and NSP2. ( a ) Yeast two-hybrid assays between NSP2 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pDEST-GADT7(AD) and pDEST-GBKT7(BD) vectors were plated onto SD/-Leu-Trp(-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP and NSP2-cYFP. There is no protein interaction in the other three combinations: MtDELLAs-nYFP--cYFP, NSP2-cYFP--nYFP and cYFP--nYFP ( Fig. 5c ). Scale bars, 20 μm.

    Article Snippet: Purification of MBP-CCaMK, HIS-IPD3, HIS-MtDELLA1, HIS-MtDELLA2 and HIS-MtDELLA3 was performed according to the protocol of E8200 (New England Biolabs) and of BioSprint96 (Qiagen), respectively.

    Techniques: Construct, Expressing, Bimolecular Fluorescence Complementation Assay, Fluorescence, Transformation Assay