hiperfect lipid transfection reagent  (Qiagen)

 
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    Name:
    HiPerFect Transfection Reagent
    Description:
    For transfection of eukaryotic cells with siRNA and miRNA Kit contents Qiagen HiPerFect Transfection Reagent 0 5mL Cationic and Neutral Lipids Technology Transient Transfection Eukaryotic Cells including Primary Cells Type Minimized Risk of Off target Effects Rapid Reverse Transfection For up to 166 Transfections in 24 well Plates or up to 666 Transfections in 96 well Plates Ideal for RNAi Studies Gene Expression Studies Benefits Efficient transfection using low siRNA concentrations Effective transfection of primary cells with high cell viability Effective transfection of suspension cells and macrophages Cell specific protocols at the TransFect Protocol Database Efficient transfection of miRNA mimics or inhib
    Catalog Number:
    301704
    Price:
    219
    Category:
    HiPerFect Transfection Reagent
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    Structured Review

    Qiagen hiperfect lipid transfection reagent
    HiPerFect Transfection Reagent
    For transfection of eukaryotic cells with siRNA and miRNA Kit contents Qiagen HiPerFect Transfection Reagent 0 5mL Cationic and Neutral Lipids Technology Transient Transfection Eukaryotic Cells including Primary Cells Type Minimized Risk of Off target Effects Rapid Reverse Transfection For up to 166 Transfections in 24 well Plates or up to 666 Transfections in 96 well Plates Ideal for RNAi Studies Gene Expression Studies Benefits Efficient transfection using low siRNA concentrations Effective transfection of primary cells with high cell viability Effective transfection of suspension cells and macrophages Cell specific protocols at the TransFect Protocol Database Efficient transfection of miRNA mimics or inhib
    https://www.bioz.com/result/hiperfect lipid transfection reagent/product/Qiagen
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    hiperfect lipid transfection reagent - by Bioz Stars, 2020-07
    99/100 stars

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    Transfection:

    Article Title: High throughput RNAi assay optimization using adherent cell cytometry
    Article Snippet: .. Significant differences were observed only when 50 nM siGLO Red and HiPerfect were used when compared to no transfection reagent. .. However, when AoSMCs were treated with RNAiMAX and siGLO Red, fluorescence pixel counts/cell were significantly higher compared to AoSMCs transfected with siGLO Red using either HiPerfect or no transfection reagent.

    Article Title: Comparison of small interfering RNA (siRNA) delivery into bovine monocyte-derived macrophages by transfection and electroporation
    Article Snippet: .. The transfection reagents; HiPerFect (Qiagen), INTERFERin (Polyplus), Lipofectamine RNAiMAX (Invitrogen), Lipofectamine 2000 (Invitrogen), DharmaFECT 1, DharmaFECT 2, DharmaFECT 3 and DharmaFECT 4 (Dharmacon), siPORT Amine (Ambion), X-tremeGENE (Roche) and N-TER (Sigma–Aldrich) were used according to the manufacturers’ instructions. .. After 16 h incubation at 37 °C the bMDM were harvested and analyzed by flow cytometry.

    Article Title: TDP-43 regulates global translational yield by splicing of exon junction complex component SKAR
    Article Snippet: .. DNA transfections were performed with FuGene6 (Roche) or Lipofectamine 2000 (Invitrogen) according to manufacturer's instruction. siRNA transfections were performed using HiPerfect (Qiagen) with 5–20 nmol siRNA. .. Scrambled siRNA (All Stars Negative Control siRNA) and all other siRNAs were purchased from Qiagen.

    Article Title: Calcium-sensing receptors signal constitutive macropinocytosis and facilitate the uptake of NOD2 ligands in macrophages
    Article Snippet: .. For siRNA transfections of hMDMs, the HiPerFect transfection reagent (Qiagen, Hilden, Germany) was used according to the manufacturer's protocol. .. For HEK cells, transfections were performed with the FuGENE 6 transfection reagent (Promega, Madison, WI, USA) according to the manufacturer's instructions.

    Article Title: High throughput RNAi assay optimization using adherent cell cytometry
    Article Snippet: .. MARCKS siRNA in the absence of a transfection reagent or complexed with HiPerfect did not lead to a significant MARCKS gene knockdown compared to control (Figure and ). ..

    Article Title: Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery
    Article Snippet: .. Knockdown of endogenous RPA2 was accomplished by siRNA transfection using HiPerFect (QIAGEN), with cells tested 72 h after transfection. .. Custom-synthesized siRNAs (GE Healthcare) used were RPA2, sense 5′-AACCUAGUUUCACAAUCUGUU-3′ and antisense 5′-CAGAUUGUGAAACUAGGUUUU-3′ (targeting the 3′-untranslated region); and PP4R2, sense 5′-UAUACUGAGAGGUCUAAUAUU-3′ and antisense 5′-UAUUAGACCUCUCAGUAUAUU-3′.

    Article Title: Sestrin-2 regulates podocyte mitochondrial dysfunction and apoptosis under high-glucose conditions via AMPK
    Article Snippet: .. Transfection sesn2-siRNA was purchased from Shanghai GenePharma Co., Ltd., and transfection was performed using the HiPerFect Transfection Reagent according to the manufacturer’s protocol (Qiagen GmbH). .. A total of 2×105 cells were seeded into six6well plates and transfected with sesn2-siRNA or a negative control scramble siRNA (Shanghai GenePharma Co., Ltd.).

    Article Title: CD36 Recruits ?5?1 Integrin to Promote Cytoadherence of P. falciparum-Infected Erythrocytes
    Article Snippet: .. The transfection mixture of 10 µl HiPerfect (Qiagen. .. GmBH, Hilden, Germany) and 20 nM siRNA for β1 integrin (Qiagen) or α5 integrin or 20 nM scrambled siRNA (All Stars Negative Control, Qiagen) was added in 100 µl of Optimem.

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    Qiagen hiperfect cationic lipid
    Hiperfect Cationic Lipid, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 451 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiperfect cationic lipid/product/Qiagen
    Average 99 stars, based on 451 article reviews
    Price from $9.99 to $1999.99
    hiperfect cationic lipid - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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