hiperfect lipid transfection reagent  (Qiagen)

 
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    Name:
    HiPerFect Transfection Reagent
    Description:
    For transfection of eukaryotic cells with siRNA and miRNA Kit contents Qiagen HiPerFect Transfection Reagent 0 5mL Cationic and Neutral Lipids Technology Transient Transfection Eukaryotic Cells including Primary Cells Type Minimized Risk of Off target Effects Rapid Reverse Transfection For up to 166 Transfections in 24 well Plates or up to 666 Transfections in 96 well Plates Ideal for RNAi Studies Gene Expression Studies Benefits Efficient transfection using low siRNA concentrations Effective transfection of primary cells with high cell viability Effective transfection of suspension cells and macrophages Cell specific protocols at the TransFect Protocol Database Efficient transfection of miRNA mimics or inhib
    Catalog Number:
    301704
    Price:
    219
    Category:
    HiPerFect Transfection Reagent
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    Structured Review

    Qiagen hiperfect lipid transfection reagent
    HiPerFect Transfection Reagent
    For transfection of eukaryotic cells with siRNA and miRNA Kit contents Qiagen HiPerFect Transfection Reagent 0 5mL Cationic and Neutral Lipids Technology Transient Transfection Eukaryotic Cells including Primary Cells Type Minimized Risk of Off target Effects Rapid Reverse Transfection For up to 166 Transfections in 24 well Plates or up to 666 Transfections in 96 well Plates Ideal for RNAi Studies Gene Expression Studies Benefits Efficient transfection using low siRNA concentrations Effective transfection of primary cells with high cell viability Effective transfection of suspension cells and macrophages Cell specific protocols at the TransFect Protocol Database Efficient transfection of miRNA mimics or inhib
    https://www.bioz.com/result/hiperfect lipid transfection reagent/product/Qiagen
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    hiperfect lipid transfection reagent - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability
    Article Snippet: .. siRNA transfections H4 cells were transiently transfected using reverse transfection with HiPerfect Transfection Reagent (Qiagen, 301705) following manufacturer’s instructions, with siRNA at 40 nM final concentration and 1:1 HiPerfect:siRNA vol:vol ratio. .. K562 cells were double transfected using Lipofectamine 2000 (Invitrogen, 11668027) following the manufacturer’s instructions, with 1ug plasmid and 50 nM final concentration siRNA with a 2:1 Lipofectamine:siRNA vol:vol ratio.

    Article Title: Favorable prognostic role of tropomodulins in neuroblastoma
    Article Snippet: .. SH-SY5Y and SK-N-SH neuroblastoma cell lines were transfected with siRNA 5 at the concentration of 10 nM using Hi-perfect transfection reagent (Qiagen) in culture medium 1 day after seeding. .. Cells were cultured for 72 hours after transfection with siRNA 5 and then collected for the mRNA expression levels analysis.

    Small Interfering RNA:

    Article Title: Volatile anesthetics suppress glucose-stimulated insulin secretion in MIN6 cells by inhibiting glucose-induced activation of hypoxia-inducible factor 1
    Article Snippet: .. Gene silencing using short interfering RNA (siRNA) siRNAs corresponding to mouse HIF-1α were from Qiagen Inc. MIN6 cells were transfected by 100 nM siRNA using HiPer-Fect™ Transfection Reagent (Qiagen) following a protocol provided by the manufacturer ( ). ..

    Transfection:

    Article Title: Volatile anesthetics suppress glucose-stimulated insulin secretion in MIN6 cells by inhibiting glucose-induced activation of hypoxia-inducible factor 1
    Article Snippet: .. Gene silencing using short interfering RNA (siRNA) siRNAs corresponding to mouse HIF-1α were from Qiagen Inc. MIN6 cells were transfected by 100 nM siRNA using HiPer-Fect™ Transfection Reagent (Qiagen) following a protocol provided by the manufacturer ( ). ..

    Article Title: The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability
    Article Snippet: .. siRNA transfections H4 cells were transiently transfected using reverse transfection with HiPerfect Transfection Reagent (Qiagen, 301705) following manufacturer’s instructions, with siRNA at 40 nM final concentration and 1:1 HiPerfect:siRNA vol:vol ratio. .. K562 cells were double transfected using Lipofectamine 2000 (Invitrogen, 11668027) following the manufacturer’s instructions, with 1ug plasmid and 50 nM final concentration siRNA with a 2:1 Lipofectamine:siRNA vol:vol ratio.

    Article Title: TDP-43 regulates global translational yield by splicing of exon junction complex component SKAR
    Article Snippet: .. DNA transfections were performed with FuGene6 (Roche) or Lipofectamine 2000 (Invitrogen) according to manufacturer's instruction. siRNA transfections were performed using HiPerfect (Qiagen) with 5–20 nmol siRNA. .. Scrambled siRNA (All Stars Negative Control siRNA) and all other siRNAs were purchased from Qiagen.

    Article Title: Phosphorylated RPA recruits PALB2 to stalled DNA replication forks to facilitate fork recovery
    Article Snippet: .. Knockdown of endogenous RPA2 was accomplished by siRNA transfection using HiPerFect (QIAGEN), with cells tested 72 h after transfection. .. Custom-synthesized siRNAs (GE Healthcare) used were RPA2, sense 5′-AACCUAGUUUCACAAUCUGUU-3′ and antisense 5′-CAGAUUGUGAAACUAGGUUUU-3′ (targeting the 3′-untranslated region); and PP4R2, sense 5′-UAUACUGAGAGGUCUAAUAUU-3′ and antisense 5′-UAUUAGACCUCUCAGUAUAUU-3′.

    Article Title: Favorable prognostic role of tropomodulins in neuroblastoma
    Article Snippet: .. SH-SY5Y and SK-N-SH neuroblastoma cell lines were transfected with siRNA 5 at the concentration of 10 nM using Hi-perfect transfection reagent (Qiagen) in culture medium 1 day after seeding. .. Cells were cultured for 72 hours after transfection with siRNA 5 and then collected for the mRNA expression levels analysis.

    Article Title: Sestrin-2 regulates podocyte mitochondrial dysfunction and apoptosis under high-glucose conditions via AMPK
    Article Snippet: .. Transfection sesn2-siRNA was purchased from Shanghai GenePharma Co., Ltd., and transfection was performed using the HiPerFect Transfection Reagent according to the manufacturer’s protocol (Qiagen GmbH). .. A total of 2×105 cells were seeded into six6well plates and transfected with sesn2-siRNA or a negative control scramble siRNA (Shanghai GenePharma Co., Ltd.).

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  • 99
    Qiagen hiperfect lipid transfection reagent
    Hiperfect Lipid Transfection Reagent, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiperfect lipid transfection reagent/product/Qiagen
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    hiperfect lipid transfection reagent - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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