Journal: Scientific Reports
Article Title: The central exons of the human MUC2 and MUC6 mucins are highly repetitive and variable in sequence between individuals
Figure Lengend Snippet: Schematic presentation of the assembled sequence for RP13-870H17 produced from PacBio SMRT and Sanger sequencing. ( a ) The RP13-870H17 assembly from SMRT sequencing results of whole BAC clone (NG-6867) and Hinf I MUC2 PTS-TR2 fragment sequence (NG-7351) assembled with Sanger sequencing. ( b ) Schematic picture of MUC6 and MUC2 gene organization showing the gene orientation and exon and intron distribution. ( c,d ) Resulting protein domain organization for MUC6 and MUC2. VWD = von Willebrand like domain type D, VWC = von Willebrand like domain type C, CK = C -terminal cystine knot.
Article Snippet: Total BAC DNA was digested with Hinf I (New England Biolabs) overnight at 37 °C according to supplier instructions.
Techniques: Sequencing, Produced, BAC Assay