hinfi  (New England Biolabs)


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    • 96
    Name:
    HinfI
    Description:

    Catalog Number:
    R0155
    Price:
    262
    Category:
    Restriction Enzymes
    Applications:
    DNA Manipulation
    Size:
    25000 units
    		Buy from Supplier

    Structured Review

    New England Biolabs hinfi
    HinfI

    https://www.bioz.com/result/hinfi/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hinfi - by Bioz Stars, 2021-07
    96/100 stars

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    Related Articles

    Methylation:

    Article Title: The Caulobacter crescentus DNA-(adenine-N6)-methyltransferase CcrM methylates DNA in a distributive manner
    Article Snippet: Methylation of pUC19 was carried out using 2 μM CcrM and 600 nM pUC19. .. To assess the methylation state of the pUC19 vector, a double digestion was carried out using HinfI and NdeI (New England Biolabs), the latter being used for linearization of the vector. ..

    Plasmid Preparation:

    Article Title: The Caulobacter crescentus DNA-(adenine-N6)-methyltransferase CcrM methylates DNA in a distributive manner
    Article Snippet: Methylation of pUC19 was carried out using 2 μM CcrM and 600 nM pUC19. .. To assess the methylation state of the pUC19 vector, a double digestion was carried out using HinfI and NdeI (New England Biolabs), the latter being used for linearization of the vector. ..

    Amplification:

    Article Title: Internal Transcribed Spacer rDNA and TEF-1α Gene Sequencing of Pathogenic Dermatophyte Species and Differentiation of Closely Related Species Using PCR-RFLP of The Topoisomerase II
    Article Snippet: Amplification profile of theproducts were also identified for all 99 strains. .. The sizeswere expected from the region amplified by dPsD2 andthe restriction enzyme digestion with Hinf I ( ) wasobtained from the website NEB cutter ( NEBcutter). .. The PCR products were digested with Hinf I. Thebanding patterns obtained by the PCR-RFLP are shown inFigure 1.

    BAC Assay:

    Article Title: The central exons of the human MUC2 and MUC6 mucins are highly repetitive and variable in sequence between individuals
    Article Snippet: Purification of Hinf I digested MUC2 PTS-TR2 fragment Hinf I is known to cut on either side of the MUC2 PTS-TR2 domain . .. Total BAC DNA was digested with Hinf I (New England Biolabs) overnight at 37 °C according to supplier instructions. .. The 7 kb band of digested BAC DNA was gel purified from a 0.7% TAE agarose gel, without UV exposure or DNA stain, using the Nucleo Spin Gel Purification kit (Macherey-Nagel) according to supplier recommendations for low concentration DNA.

    Agarose Gel Electrophoresis:

    Article Title: Trypanosoma brucei UMSBP2 is a single-stranded telomeric DNA binding protein essential for chromosome end protection
    Article Snippet: In-gel hybridization analysis of single-stranded telomeric DNA In-gel hybridization was performed following ( ). .. Genomic DNA samples (1–1.5 μg) were digested with restriction endonucleases HinfI or HinfI and AluI (NEB Inc.) and separated on a 0.7% agarose gel. .. The samples were run in duplicates for hybridization with C-rich and G-rich telomeric probes in parallel.

    Combined Bisulfite Restriction Analysis Assay:

    Article Title: Discovery of DNA methylation markers in cervical cancer using relaxation ranking
    Article Snippet: PCR primers for amplification of specific targets sequences are listed in Additional file . .. COBRA was performed directly on the BSP products as described by Xiong et al. [ ]. using digestions with BstUI , Taq1 and/or HinfI according the manufacture's protocol (New England Biolabs Inc., Beverly, MA). .. For sequence analysis, the BSP products were purified (Qiagen, Westburg, Leusden, the Netherlands) and subjected to direct sequencing (BaseClear, Leiden, the Netherlands).

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    • hinf i  (New England Biolabs)
    96
    New England Biolabs hinf i
    Schematic presentation of the assembled sequence for RP13-870H17 produced from PacBio SMRT and Sanger sequencing. ( a ) The RP13-870H17 assembly from SMRT sequencing results of whole <t>BAC</t> clone (NG-6867) and <t>Hinf</t> I MUC2 PTS-TR2 fragment sequence (NG-7351) assembled with Sanger sequencing. ( b ) Schematic picture of MUC6 and MUC2 gene organization showing the gene orientation and exon and intron distribution. ( c,d ) Resulting protein domain organization for MUC6 and MUC2. VWD = von Willebrand like domain type D, VWC = von Willebrand like domain type C, CK = C -terminal cystine knot.
    Hinf I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hinf i/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hinf i - by Bioz Stars, 2021-07
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Schematic presentation of the assembled sequence for RP13-870H17 produced from PacBio SMRT and Sanger sequencing. ( a ) The RP13-870H17 assembly from SMRT sequencing results of whole BAC clone (NG-6867) and Hinf I MUC2 PTS-TR2 fragment sequence (NG-7351) assembled with Sanger sequencing. ( b ) Schematic picture of MUC6 and MUC2 gene organization showing the gene orientation and exon and intron distribution. ( c,d ) Resulting protein domain organization for MUC6 and MUC2. VWD = von Willebrand like domain type D, VWC = von Willebrand like domain type C, CK = C -terminal cystine knot.

    Journal: Scientific Reports

    Article Title: The central exons of the human MUC2 and MUC6 mucins are highly repetitive and variable in sequence between individuals

    doi: 10.1038/s41598-018-35499-w

    Figure Lengend Snippet: Schematic presentation of the assembled sequence for RP13-870H17 produced from PacBio SMRT and Sanger sequencing. ( a ) The RP13-870H17 assembly from SMRT sequencing results of whole BAC clone (NG-6867) and Hinf I MUC2 PTS-TR2 fragment sequence (NG-7351) assembled with Sanger sequencing. ( b ) Schematic picture of MUC6 and MUC2 gene organization showing the gene orientation and exon and intron distribution. ( c,d ) Resulting protein domain organization for MUC6 and MUC2. VWD = von Willebrand like domain type D, VWC = von Willebrand like domain type C, CK = C -terminal cystine knot.

    Article Snippet: Total BAC DNA was digested with Hinf I (New England Biolabs) overnight at 37 °C according to supplier instructions.

    Techniques: Sequencing, Produced, BAC Assay

    Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) electrophoretic patterns of dermatophytes species by amplification of topoisomerase II gene and digestion of the Hinf I enzyme. Lane M; 100 bp DNA ladder, Lane 1; T. tonsurans, Lane 2; T. tonsurans (CBS 130924), Lane 3; T. interdigitale , Lane 4; T. mentagrophytes, Lane 5; T. rubrum, Lane 6; T. rubrum (PFCC 51431), Lane 7; E. floccosum , and Lane 8; E. floccosum (CBS 767.73).

    Journal: Cell Journal (Yakhteh)

    Article Title: Internal Transcribed Spacer rDNA and TEF-1α Gene Sequencing of Pathogenic Dermatophyte Species and Differentiation of Closely Related Species Using PCR-RFLP of The Topoisomerase II

    doi: 10.22074/cellj.2020.6372

    Figure Lengend Snippet: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) electrophoretic patterns of dermatophytes species by amplification of topoisomerase II gene and digestion of the Hinf I enzyme. Lane M; 100 bp DNA ladder, Lane 1; T. tonsurans, Lane 2; T. tonsurans (CBS 130924), Lane 3; T. interdigitale , Lane 4; T. mentagrophytes, Lane 5; T. rubrum, Lane 6; T. rubrum (PFCC 51431), Lane 7; E. floccosum , and Lane 8; E. floccosum (CBS 767.73).

    Article Snippet: The sizeswere expected from the region amplified by dPsD2 andthe restriction enzyme digestion with Hinf I ( ) wasobtained from the website NEB cutter ( NEBcutter).

    Techniques: Polymerase Chain Reaction, Amplification

    Restriction enzyme digestion of the positive LAMP products. ( A ) Schematic representation of the anticipated restricted DNA products. B+, B−, F+, F−,+ and-in the first row represent the same regions described by Notomi (Notomi et al . 4 ). ( B ) LAMP products were digested with Ase I and Hinf I, and the fragments were observed by 2.0% agarose gel electrophoresis.

    Journal: Scientific Reports

    Article Title: Development and application of loop-mediated isothermal amplification (LAMP) for detection of Plasmopara viticola

    doi: 10.1038/srep28935

    Figure Lengend Snippet: Restriction enzyme digestion of the positive LAMP products. ( A ) Schematic representation of the anticipated restricted DNA products. B+, B−, F+, F−,+ and-in the first row represent the same regions described by Notomi (Notomi et al . 4 ). ( B ) LAMP products were digested with Ase I and Hinf I, and the fragments were observed by 2.0% agarose gel electrophoresis.

    Article Snippet: The LAMP products were digested with the restriction enzymes Ase I and Hinf I (New England Biolabs) according to the operating instructions.

    Techniques: Agarose Gel Electrophoresis

    Three percent agarose gel, Hinf I restriction fragment length polymorphism analysis of methylenetetrahydrofolate reductase 677; Lane 1-50 bp ladder; Lanes 2, 3, 4, 6 and 8 - homozygous wild type; Lane 5 and 7 -heterozygous polymorphic

    Journal: Indian Journal of Human Genetics

    Article Title: Methylenetetrahydrofolate reductase C677T variant in Indian children with craniosynostosis: Its role in the pathogenesis, risk of craniosynostosis

    doi: 10.4103/0971-6866.142882

    Figure Lengend Snippet: Three percent agarose gel, Hinf I restriction fragment length polymorphism analysis of methylenetetrahydrofolate reductase 677; Lane 1-50 bp ladder; Lanes 2, 3, 4, 6 and 8 - homozygous wild type; Lane 5 and 7 -heterozygous polymorphic

    Article Snippet: After successful amplification, a small aliquot (5 μl) of the MTHFR reaction mixture was treated with 1 units of Hinf I restriction enzyme (NEB).

    Techniques: Agarose Gel Electrophoresis

    Two percent agarose gel, polymerase chain reaction (PCR) product before Hinf I restriction fragment length polymorphism; Lane 1 - 100 bp ladder; Lane 2 - Blank; Lanes 3, 4, 5, 6, 7 and 8 - PCR product of 315 bp

    Journal: Indian Journal of Human Genetics

    Article Title: Methylenetetrahydrofolate reductase C677T variant in Indian children with craniosynostosis: Its role in the pathogenesis, risk of craniosynostosis

    doi: 10.4103/0971-6866.142882

    Figure Lengend Snippet: Two percent agarose gel, polymerase chain reaction (PCR) product before Hinf I restriction fragment length polymorphism; Lane 1 - 100 bp ladder; Lane 2 - Blank; Lanes 3, 4, 5, 6, 7 and 8 - PCR product of 315 bp

    Article Snippet: After successful amplification, a small aliquot (5 μl) of the MTHFR reaction mixture was treated with 1 units of Hinf I restriction enzyme (NEB).

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction