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Agilent technologies hilic
Hilic, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hilic/product/Agilent technologies
Average 91 stars, based on 16 article reviews
Price from $9.99 to $1999.99
hilic - by Bioz Stars, 2020-07
91/100 stars

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Hydrophilic Interaction Liquid Chromatography:

Article Title: Gadolinium Tagged Osteoprotegerin-Mimicking Peptide: A Novel Magnetic Resonance Imaging Biospecific Contrast Agent for the Inhibition of Osteoclastogenesis and Osteoclast Activity
Article Snippet: .. The peptide synthesis products were separated by HILIC on an HP 1200 HPLC (Agilent Technologies, Santa Clara, CA, USA) equipped with a TSK-Gel Amide 80 HILIC column (250 mm (length) × 4.6 mm internal diameter and 5 μm particle size) from TOSOH Bioscience (Tokyo, Japan). .. The system used a binary mobile phase of solvent A (acetonitrile, 0.1% ( v / v ) formic acid) and solvent B (water, 0.1% ( v / v ) formic acid) at a flow rate of 300 µL/min.

Article Title: The metabolome regulates the epigenetic landscape during naïve to primed human embryonic stem cell transition
Article Snippet: .. HILIC and Reversed Phase LC-QTOF analysis and data processing was performed as previously described using an Agilent 1200 series HPLC equipped with either Agilent Zorbax Eclipse Plus C18 2.1×150mm column for Reversed Phase or a Waters 1.7µm Acquity BEH HILIC 2.1×150mm column. .. LC eluents were analyzed with an Agilent 6530 accurate mass Q-TOF mass spectrometer.

High Performance Liquid Chromatography:

Article Title: Gadolinium Tagged Osteoprotegerin-Mimicking Peptide: A Novel Magnetic Resonance Imaging Biospecific Contrast Agent for the Inhibition of Osteoclastogenesis and Osteoclast Activity
Article Snippet: .. The peptide synthesis products were separated by HILIC on an HP 1200 HPLC (Agilent Technologies, Santa Clara, CA, USA) equipped with a TSK-Gel Amide 80 HILIC column (250 mm (length) × 4.6 mm internal diameter and 5 μm particle size) from TOSOH Bioscience (Tokyo, Japan). .. The system used a binary mobile phase of solvent A (acetonitrile, 0.1% ( v / v ) formic acid) and solvent B (water, 0.1% ( v / v ) formic acid) at a flow rate of 300 µL/min.

Article Title: The metabolome regulates the epigenetic landscape during naïve to primed human embryonic stem cell transition
Article Snippet: .. HILIC and Reversed Phase LC-QTOF analysis and data processing was performed as previously described using an Agilent 1200 series HPLC equipped with either Agilent Zorbax Eclipse Plus C18 2.1×150mm column for Reversed Phase or a Waters 1.7µm Acquity BEH HILIC 2.1×150mm column. .. LC eluents were analyzed with an Agilent 6530 accurate mass Q-TOF mass spectrometer.

other:

Article Title: Metabolomics and transcriptomics pathway approach reveals outcome-specific perturbations in COPD
Article Snippet: Tandem mass spectrometry (MSMS) The HILIC and C18 chromatographic methods discussed above were replicated for LC-MS/MS analysis using 10, 20, and 40 eV collision energies on a 6520 Q-TOF-MS (Agilent) with a 500 ms/spectra acquisition time, 4 m/z isolation width, and 1 minute delta retention time.

Article Title: Keratan sulfate glycosaminoglycan from chicken egg white
Article Snippet: LC relied on a Luna® 3 µm HILIC 200 Å column (150 × 2 mm) attached to an Agilent 1200 HPLC coupled with on-line detection using an LTQ-Orbitrap XL FT MS (Thermo Fisher Scientific) was equipped with ESI source with a spray voltage of 4.2 kV, a capillary voltage of −40 V, a tube lens voltage of −50 V, a capillary temperature of 275°C, a sheath flow rate of 30 and an auxiliary gas flow rate of 6.

Article Title: Metabolomic similarities between bronchoalveolar lavage fluid and plasma in humans and mice
Article Snippet: MS/MS analysis The HILIC, C18 and SB-AQ chromatographic methods were replicated for LC-MS/MS analysis using 10, 20, and 40 eV collision energies on a 6520 Q-TOF (Agilent) with a 500 ms/spectra acquisition time, 4 m/z isolation width, and 1 minute delta retention time.

Article Title: SUMOylation-regulated Protein Phosphorylation, Evidence from Quantitative Phosphoproteomics Analyses *
Article Snippet: Phosphopeptides fractionation by HILIC was performed on an Agilent 1200 HPLC system (Agilent Technologies) equipped with a TSKgel Amide-80 column (2.0 × 150 mm, 5-μm particle size, 200-Å pore size) (TOSOH Bioscience).

Article Title: In-Depth Quantitative Proteomic Analysis of de Novo Protein Synthesis Induced by Brain-Derived Neurotrophic Factor
Article Snippet: All HILIC experiments were performed on an Agilent 1100 HPLC system using a 1 × 250 mm TSKgel Amide-80 5 μm particle column (Tosoh Biosciences).

Article Title: Metabolomics and transcriptomics pathway approach reveals outcome-specific perturbations in COPD
Article Snippet: The HILIC and C18 chromatographic methods discussed above were replicated for LC-MS/MS analysis using 10, 20, and 40 eV collision energies on a 6520 Q-TOF-MS (Agilent) with a 500 ms/spectra acquisition time, 4 m/z isolation width, and 1 minute delta retention time.

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  • 91
    Agilent technologies hilic column
    Phosphoenolpyruvate Accumulates in Cells with Low PYK Activity and Inhibits Triosephosphate Isomerase (A) <t>HILIC-MRM</t> quantification of <t>PEP</t> (left panel) Standard addition of PEP to whole-cell methanol/water yeast extracts; and quantification of PEP separated by HILIC using multiple reaction monitoring (MRM). Quantification is demonstrated by linear regression, R 2 vales of > 0.97 were obtained. (Right panel) HILIC-MRM quantification of PEP in TEF pr -PYK1 , CYC pr -PYK1 , and CYC pr -PYK2 yeast; PEP is strongly accumulated in yeast with low PYK activity. Error bars, ±SD, n = 3. (B) PEP inactivates yeast and mammalian TPI. Michaelis-Menten kinetics were determined for yeast TPI (left panel), human TPI (middle panel), and rabbit muscle TPI (right panel). To determine v max and K m , the TPI substrate gly3p was added in incremental doses (left y and lower x axis, blue; y values are normalized to total cellular protein [left and middle panel] or to the purified protein [right panel]). For all TPI isozymes, v max is highlighted with a green dot in the saturation curve; K m values are given in μM (right y and upper x axis, black). Inhibition of TPI activity demonstrated by addition of PEP in incremental concentrations; IC 50 values and the inhibitory constant K i are given in μM, the Kcat in Mol ∗ s −1 . (C) Inactivation of pathogenic TPI alleles by PEP. TPI activity was assayed in transgenic yeast expressing human TPI (WT) or indicated pathogenic TPI alleles without or in the presence of 900 μmol PEP. Error bars, ±SD. Values within bars indicate the absolute enzyme activity in μmol/(min ∗ μg protein). (D) PYK activity does not change oxidant resistance in yeast expressing TPI Ile170Val . The wild-type control, transgenic yeast expressing either PYK1 or PYK2 , and either human TPI or human TPI Ile170Val were spotted as serial dilutions onto SC media without or with diamide (left panel). Spot growth on different concentrations was analyzed with CellProfiler and normalized to the strain with highest PYK activity (right panel). Low PYK activity increased diamide tolerance if expressed in combination with human TPI, but there were no differences in TPI Ile170Val -expressing yeast.
    Hilic Column, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic column/product/Agilent technologies
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    hilic column - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    85
    Agilent technologies hilic dad
    <t>HILIC-DAD</t> chromatograms of Cordyceps extract ( 1 ) Thymine; ( 2 ) Uracil; ( 3 )Thymidine; ( 4 ) 2'-Deoxyuridine; ( 5 ) Cordycepin; ( 6 ) Uridine; ( 7 ) Hypoxanthine; ( 8 ) Adenine; ( 9 ) Adenosine; ( 10 )Xanthine; ( 11 ) Inosine; ( 12 ) Cytosine; ( 13 ) Guanine; ( 14 ) Cytidine; ( 15 ) Guanosine;(N1-N8: non-target compounds).
    Hilic Dad, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic dad/product/Agilent technologies
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hilic dad - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    92
    Agilent technologies hilic ftms analysis
    TICs of depolymerized FCS by partial N -deacetylation–deaminative cleavage from four species of sea cucumbers profiled by <t>HILIC-FTMS.</t>
    Hilic Ftms Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic ftms analysis/product/Agilent technologies
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    hilic ftms analysis - by Bioz Stars, 2020-07
    92/100 stars
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    85
    Agilent technologies hilic esi ms ms analysis quantitative analyses
    <t>HILIC-ESI/TOF/MS</t> chromatograms for Cordyceps extract.
    Hilic Esi Ms Ms Analysis Quantitative Analyses, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hilic esi ms ms analysis quantitative analyses/product/Agilent technologies
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hilic esi ms ms analysis quantitative analyses - by Bioz Stars, 2020-07
    85/100 stars
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    Phosphoenolpyruvate Accumulates in Cells with Low PYK Activity and Inhibits Triosephosphate Isomerase (A) HILIC-MRM quantification of PEP (left panel) Standard addition of PEP to whole-cell methanol/water yeast extracts; and quantification of PEP separated by HILIC using multiple reaction monitoring (MRM). Quantification is demonstrated by linear regression, R 2 vales of > 0.97 were obtained. (Right panel) HILIC-MRM quantification of PEP in TEF pr -PYK1 , CYC pr -PYK1 , and CYC pr -PYK2 yeast; PEP is strongly accumulated in yeast with low PYK activity. Error bars, ±SD, n = 3. (B) PEP inactivates yeast and mammalian TPI. Michaelis-Menten kinetics were determined for yeast TPI (left panel), human TPI (middle panel), and rabbit muscle TPI (right panel). To determine v max and K m , the TPI substrate gly3p was added in incremental doses (left y and lower x axis, blue; y values are normalized to total cellular protein [left and middle panel] or to the purified protein [right panel]). For all TPI isozymes, v max is highlighted with a green dot in the saturation curve; K m values are given in μM (right y and upper x axis, black). Inhibition of TPI activity demonstrated by addition of PEP in incremental concentrations; IC 50 values and the inhibitory constant K i are given in μM, the Kcat in Mol ∗ s −1 . (C) Inactivation of pathogenic TPI alleles by PEP. TPI activity was assayed in transgenic yeast expressing human TPI (WT) or indicated pathogenic TPI alleles without or in the presence of 900 μmol PEP. Error bars, ±SD. Values within bars indicate the absolute enzyme activity in μmol/(min ∗ μg protein). (D) PYK activity does not change oxidant resistance in yeast expressing TPI Ile170Val . The wild-type control, transgenic yeast expressing either PYK1 or PYK2 , and either human TPI or human TPI Ile170Val were spotted as serial dilutions onto SC media without or with diamide (left panel). Spot growth on different concentrations was analyzed with CellProfiler and normalized to the strain with highest PYK activity (right panel). Low PYK activity increased diamide tolerance if expressed in combination with human TPI, but there were no differences in TPI Ile170Val -expressing yeast.

    Journal: Cell Metabolism

    Article Title: Pyruvate Kinase Triggers a Metabolic Feedback Loop that Controls Redox Metabolism in Respiring Cells

    doi: 10.1016/j.cmet.2011.06.017

    Figure Lengend Snippet: Phosphoenolpyruvate Accumulates in Cells with Low PYK Activity and Inhibits Triosephosphate Isomerase (A) HILIC-MRM quantification of PEP (left panel) Standard addition of PEP to whole-cell methanol/water yeast extracts; and quantification of PEP separated by HILIC using multiple reaction monitoring (MRM). Quantification is demonstrated by linear regression, R 2 vales of > 0.97 were obtained. (Right panel) HILIC-MRM quantification of PEP in TEF pr -PYK1 , CYC pr -PYK1 , and CYC pr -PYK2 yeast; PEP is strongly accumulated in yeast with low PYK activity. Error bars, ±SD, n = 3. (B) PEP inactivates yeast and mammalian TPI. Michaelis-Menten kinetics were determined for yeast TPI (left panel), human TPI (middle panel), and rabbit muscle TPI (right panel). To determine v max and K m , the TPI substrate gly3p was added in incremental doses (left y and lower x axis, blue; y values are normalized to total cellular protein [left and middle panel] or to the purified protein [right panel]). For all TPI isozymes, v max is highlighted with a green dot in the saturation curve; K m values are given in μM (right y and upper x axis, black). Inhibition of TPI activity demonstrated by addition of PEP in incremental concentrations; IC 50 values and the inhibitory constant K i are given in μM, the Kcat in Mol ∗ s −1 . (C) Inactivation of pathogenic TPI alleles by PEP. TPI activity was assayed in transgenic yeast expressing human TPI (WT) or indicated pathogenic TPI alleles without or in the presence of 900 μmol PEP. Error bars, ±SD. Values within bars indicate the absolute enzyme activity in μmol/(min ∗ μg protein). (D) PYK activity does not change oxidant resistance in yeast expressing TPI Ile170Val . The wild-type control, transgenic yeast expressing either PYK1 or PYK2 , and either human TPI or human TPI Ile170Val were spotted as serial dilutions onto SC media without or with diamide (left panel). Spot growth on different concentrations was analyzed with CellProfiler and normalized to the strain with highest PYK activity (right panel). Low PYK activity increased diamide tolerance if expressed in combination with human TPI, but there were no differences in TPI Ile170Val -expressing yeast.

    Article Snippet: PEP was extracted with methanol/water and separated on a HILIC column (1.7 μm particle size, 2.1 × 100 mm) using ultra-high-pressure binary pump (Agilent 1290) at 800–1000 bar and a flow rate of 1 ml/min.

    Techniques: Activity Assay, Hydrophilic Interaction Liquid Chromatography, Standard Addition, Purification, Inhibition, Transgenic Assay, Expressing

    HILIC-DAD chromatograms of Cordyceps extract ( 1 ) Thymine; ( 2 ) Uracil; ( 3 )Thymidine; ( 4 ) 2'-Deoxyuridine; ( 5 ) Cordycepin; ( 6 ) Uridine; ( 7 ) Hypoxanthine; ( 8 ) Adenine; ( 9 ) Adenosine; ( 10 )Xanthine; ( 11 ) Inosine; ( 12 ) Cytosine; ( 13 ) Guanine; ( 14 ) Cytidine; ( 15 ) Guanosine;(N1-N8: non-target compounds).

    Journal: Molecules

    Article Title: Characterization of Nucleosides and Nucleobases in Natural Cordyceps by HILIC–ESI/TOF/MS and HILIC–ESI/MS

    doi: 10.3390/molecules18089755

    Figure Lengend Snippet: HILIC-DAD chromatograms of Cordyceps extract ( 1 ) Thymine; ( 2 ) Uracil; ( 3 )Thymidine; ( 4 ) 2'-Deoxyuridine; ( 5 ) Cordycepin; ( 6 ) Uridine; ( 7 ) Hypoxanthine; ( 8 ) Adenine; ( 9 ) Adenosine; ( 10 )Xanthine; ( 11 ) Inosine; ( 12 ) Cytosine; ( 13 ) Guanine; ( 14 ) Cytidine; ( 15 ) Guanosine;(N1-N8: non-target compounds).

    Article Snippet: HILIC-DAD All separation processes were performed on an Agilent 1200 HPLC system (Agilent Technologies, Santa Clara, CA, USA) equipped with a quaternary solvent delivery system, a vacuum degasser, an autosampler, a column oven, and a diode-array detector connected to an Agilent ChemStation software program.

    Techniques: Hydrophilic Interaction Liquid Chromatography

    TICs of depolymerized FCS by partial N -deacetylation–deaminative cleavage from four species of sea cucumbers profiled by HILIC-FTMS.

    Journal: Glycobiology

    Article Title: Bottom-up analysis using liquid chromatography–Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers

    doi: 10.1093/glycob/cwz057

    Figure Lengend Snippet: TICs of depolymerized FCS by partial N -deacetylation–deaminative cleavage from four species of sea cucumbers profiled by HILIC-FTMS.

    Article Snippet: Depolymerized FCS samples were dissolved in 50% acetonitrile as 1 mg/mL for HILIC-FTMS analysis, which was performed on an Agilent 1290 LC UPLC system (Agilent Technologies, Wilmington, DE) equipped with a LTQ ORBITRAP XL mass spectrometer (Thermo, SCIENTIFIC).

    Techniques: Hydrophilic Interaction Liquid Chromatography

    HILIC-ESI/TOF/MS chromatograms for Cordyceps extract.

    Journal: Molecules

    Article Title: Characterization of Nucleosides and Nucleobases in Natural Cordyceps by HILIC–ESI/TOF/MS and HILIC–ESI/MS

    doi: 10.3390/molecules18089755

    Figure Lengend Snippet: HILIC-ESI/TOF/MS chromatograms for Cordyceps extract.

    Article Snippet: HILIC-ESI-MS/MS Analysis Quantitative analyses of nucleosides and nucleobases were performed on an Agilent 1200 HPLC system (Agilent Technologies) equipped with a Waters Xbridge Amide column (250 × 4.6 mm I.D., 3.5 μm) and coupled to an Agilent G6420A mass spectrometer (Agilent Technologies) triple quadrupole instrument.

    Techniques: Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry